CN108840902A - Canned sardines processes the recovery method of fish protein and its application in fish soup waste liquid - Google Patents

Canned sardines processes the recovery method of fish protein and its application in fish soup waste liquid Download PDF

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Publication number
CN108840902A
CN108840902A CN201810773307.3A CN201810773307A CN108840902A CN 108840902 A CN108840902 A CN 108840902A CN 201810773307 A CN201810773307 A CN 201810773307A CN 108840902 A CN108840902 A CN 108840902A
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China
Prior art keywords
fish
waste liquid
fish protein
protein
recovery method
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Pending
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CN201810773307.3A
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Chinese (zh)
Inventor
郑宗平
叶南团
郑瑞生
郭凤仙
林娈
陈文静
章超桦
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Quanzhou Normal University
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Quanzhou Normal University
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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/145Extraction; Separation; Purification by extraction or solubilisation
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17Amino acids, peptides or proteins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/30Extraction; Separation; Purification by precipitation
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Mycology (AREA)
  • Nutrition Science (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Meat, Egg Or Seafood Products (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention provides the recovery method of fish protein in a kind of canned sardines processing fish soup waste liquid and its applications, belong to food technology field.Canned sardines processes fish soup waste liquid and carries out degreasing with n-hexane, and hydrochloric acid tune pH value is added in the fish soup after degreasing makes it generate precipitating, then by precipitation and centrifugal separation, drying to get fish protein.The present invention obtains the fish protein in fish soup waste liquid using salting-out method, has technically simple, easy to industrialized production advantage.Gained fish protein can be individually used for being processed into fish protein powder, or make an addition in the food such as biscuit, bread, cake, flavouring as nutritional ingredient, enhance food nutrition and flavor.The present invention is greatly improved the utilization rate of resource, reduces environmental pollution, and improves the added value of fish soup waste material, increases the profit of enterprise.

Description

Canned sardines processes the recovery method of fish protein and its application in fish soup waste liquid
Technical field
The invention belongs to food processing technology fields, and in particular to canned sardines processes fish protein in fish soup waste liquid Recovery method and its application.
Background technique
The development of China's fish processing industry is swift and violent year by year, the processing of the marine fishes such as hairtail, little yellow croaker, sardine Yield increasingly increases.Show China's total aquatic product production in 2014 up to 6.461x 10 according to correlative study7Ton, Mesichthyes total yield Amount has reached 2.711x107Ton, accounts for about the 33.33% of world's fish total output, and leaps within continuous 22 years first, the whole world, still Processing of aquatic products rate only 35.1%.In marine fishes processing waste material, protein, fish oil, microelement equal size are higher, and price is low It is honest and clean, it is a kind of good protein sources, nitrogen source.Currently, China to technologies such as the processing, recycling and storage of fish processing waste material also not Enough mature and correlative studys are relatively fewer.In the processing mode of dead meal, often by fish processing waste material be prepared into minced fillet, The products such as feeding fish meal directly abandon, and fish soup waste material is exhausted directly to sewer mostly, causes a large amount of fish processing wastes Utilization rate is lower, this not only causes the waste of resource, but also causes strong influence to ecological environment.Therefore, how to realize Fish processing waste material resource height becomes current letter problem to be solved using oneself.With the swift and violent hair of modern marine Fishing technology Exhibition, the development of marine fishes processing industry are already also come out top.The processing dead meal generated in marine fishes process also day Benefit increases, and the utilization and its exploitation of Related product of fish processing dead meal are gradually paid close attention to by people, fish by-product it is fine It processes to diversification, systematization and high-valued draws close.With being incremented by for population, the shortage of food rationally utilizes existing resource Seem increasingly important.The recycling of marine fishes processing waste be advantageously implemented ocean fishing class processing industry to high economic benefit, The target of low environment pollution.
Supplement protein can be reduced the destruction of musculature, promote the synthesis or even overrecovery of albumen, to make muscle Increased, strength is improved.But traditional method often takes in excess fat by eating meat supplement protein, causes body Rouge increases, or even influences cardiovascular health.And fish protein fat content is low, absorption easy to digest, is a kind of ideal battalion Form part.In production canned sardines, the fish soup of infusion sardine is given up usually as waste liquid, not only will increase the place of enterprise The economic cost for managing sewage, can also cause environment greatly to pollute.Fish soup waste material in canned sardines manufacturing process is returned It receives and utilizes, extract the fish protein with high economic value, the economic benefit of enterprise can not only be improved, also had to environmental protection Significance.
Summary of the invention
The purpose of the present invention is to provide a kind of canned sardines processing fish soup waste liquid in fish protein recovery method and It is applied
The purpose of the present invention is to recycle fish protein from canned sardines processing fish soup waste liquid first, and canned sardines adds Work fish soup waste liquid carries out degreasing with n-hexane, and hydrochloric acid tune pH value is added in the fish soup after degreasing, makes to generate sediment, sediment It is centrifuged, dries to get fish protein, purity is up to 40~50%.
The recovery method of above-mentioned recycling fish protein, specifically includes following steps:
(1) n-hexane extraction 2 times of canned sardines processing 2-2.5 times of quality of fish soup waste liquid carry out degreasing, to extraction Upper solution afterwards recycles n-hexane, and it is stand-by to collect lower aqueous solution extracted;
(2) lower aqueous solution is placed in test tube, the pH value that lower aqueous solution is adjusted with HCl solution is 3.5-4.5, quiet 20-30min is set, is then centrifuged for, upper solution is discarded, collects sediment;
(3) sediment taking-up is placed in culture dish, is placed in freeze drier and carries out low-temperature reduced-pressure drying, obtains Fish protein Matter.
The molar concentration of the HCl solution is 0.1-0.15mol/L.
Preferably, the pH for adjusting lower aqueous solution is 4.0, which is the optimal pH so that Fish protein precipitating.
The centrifugal condition is 4 DEG C, and 6500-7500r/min is centrifuged 20-25min.
The temperature of the vacuum freeze drying is -78 DEG C, vacuum degree<5Pa.
Another object of the present invention is applied to Fish protein as a kind of nutrition in food.Above-mentioned recycling side The fish protein that method obtains is powder or bulk, and fish protein powder is broken to appropriate mesh number further processing as needed and is utilized, one As be crushed to 100-200 mesh.
Fish protein of the invention can be used as nutritional ingredient, is not added or is added food ingredient, food additives auxiliary material, press Take a picture in requisition for preparation method product is made.The product include fish protein powder, biscuit, bread, cake, cookies, cake class, Steamed stuffed bun, steamed bun, noodles, bean vermicelli, egg roll, fish meal etc..
The invention has the beneficial effects that:The fish protein powder for being rich in protein is provided, body long-term lacking albumen can be corrected Matter and caused by malnutrition, and gastrointestinal disorders can be corrected, improve digestive function, while can sufficiently provide skin again Nutrient needed for raw, promotes the maintenance ability of skin, delay skin aging increases the elasticity and thickness of skin, improves the guarantor of skin Temperature function reduces face wrinkles, removes sunburn, skin is made to become soft and moist smooth.Fish protein can have powdery, liquid and paste The variforms such as shape, convenient for according to the need of crowd's nutritional character and market under the conditions of the crowd of different physiological status, different work It pleads condition, is thoroughly mixed together with other food materials, is reprocessed into colourful marine food, this meets current work The trend of journey food.The exploitation of fish protein powder can reduce the wasting of resources, improve its economic value and utility value.
Specific embodiment
Below in conjunction with specific embodiment the substantive content and beneficial effect that present invention be described in more detail, these implementations Example is merely to illustrate the present invention rather than limitation of the present invention.In addition, after reading the content taught by the present invention, this field Technical staff can make various changes or modifications the present invention, and such equivalent forms equally fall within the application the appended claims Limited range.
Embodiment 1
A kind of canned sardines processes the recovery method of fish protein in fish soup waste liquid, includes the following steps:
(1) n-hexane extraction 2 times of canned sardines processing 2 times of quality of fish soup waste liquid carry out degreasing, to extracted Upper solution recycles n-hexane, and it is stand-by to collect lower aqueous solution extracted;
(2) lower aqueous solution is placed in test tube, is with the pH value that 0.1mol/L HCl solution adjusts lower aqueous solution 4.0,25min is stood, then 4 DEG C, 7000r/min is centrifuged 20min, discards upper solution, collects sediment;
(3) sediment taking-up is placed in culture dish, is placed in freeze drier, at -78 DEG C of temperature, vacuum degree<5Pa's Under the conditions of to carry out low-temperature reduced-pressure dry, obtain fish protein.
Embodiment 2
A kind of canned sardines processes the recovery method of fish protein in fish soup waste liquid, includes the following steps:
(1) n-hexane extraction 2 times of canned sardines processing 2.5 times of quality of fish soup waste liquid carry out degreasing, after extraction Upper solution recycle n-hexane, it is stand-by to collect lower aqueous solution extracted;
(2) lower aqueous solution is placed in test tube, is with the pH value that 0.15mol/L HCl solution adjusts lower aqueous solution 4.5,20min is stood, then 4 DEG C, 6500r/min is centrifuged 25min, discards upper solution, collects sediment;
(3) sediment taking-up is placed in culture dish, is placed in freeze drier, at -78 DEG C of temperature, vacuum degree<5Pa's Under the conditions of to carry out low-temperature reduced-pressure dry, obtain fish protein.
Embodiment 3
A kind of canned sardines processes the recovery method of fish protein in fish soup waste liquid, includes the following steps:
(1) n-hexane extraction 2 times of canned sardines processing 2.2 times of quality of fish soup waste liquid carry out degreasing, after extraction Upper solution recycle n-hexane, it is stand-by to collect lower aqueous solution extracted;
(2) lower aqueous solution is placed in test tube, is with the pH value that 0.12mol/L HCl solution adjusts lower aqueous solution 3.5,30min is stood, then 4 DEG C, 7500r/min is centrifuged 20min, discards upper solution, collects sediment;
(3) sediment taking-up is placed in culture dish, is placed in freeze drier, at -78 DEG C of temperature, vacuum degree<5Pa's Under the conditions of to carry out low-temperature reduced-pressure dry, obtain fish protein.
Application examples 1
Application of the fish protein in biscuit
1 fish protein powder cookie formulation of table
Formula is shown in Table 1.Key points for operation:(1) biscuit paste is beaten:Egg cleans decladding, egg liquid, vanilla powder and 250g white granulated sugar Be placed in stainless steel circular bottom pot, get angry heating, break into bulk body with laing eggs, be warmed to 50 DEG C or so remove fire continue beat to it is cold when, Beat it is thick, 500g flour and 100g fish protein powder sieving be poured in egg pot, stir, stirred evenly i.e. in stopping surface powder, fish protein powder At biscuit paste.(2) it forms, toast:Iron baking tray is wiped clean, one layer of vegetable oil is spread, then spreads a level powder (i.e. with 50g flour It is sprinkling upon on baking tray), biscuit paste is packed into the cloth bag with circle mouth, the right hand grabs the suitable for reading of cloth pocket, and left hand pinches cloth pocket Lower mouth circle mouthpiece arranges distance by ranks sequence, squeezes on the baking tray for having spread flour, after having squeezed, remaining 50g white sand Sugar is sprinkling upon on the biscuit squeezed one layer, and baking tray is placed in dry place, biscuit is made to dry skin.It is sent into 180 DEG C of ovens and bakes 10min, cake It is dry be presented above it is light yellow, i.e., it is ripe, come out of the stove.
Application examples 2
Application of the fish protein in pocket bread
2 fish protein powder pocket bread formula of table
Formula is shown in Table 2.Make main points:After the above formula mixing, stirring to gluten extension phase, dough temperature keeps 25 DEG C, fermentation 150min substantially, when being sent to 105min, turn-over is primary, intermediate provocation 25min after question handler segmentation is round as a ball.Shaping dress It is put into last fermenting cellar after disk, is then toasted, 200 DEG C of oven temperatures, bakes 30min.It is i.e. ripe, it comes out of the stove.Application examples 3
Fish protein powder
3 fish protein powder formula of table
Formula is shown in Table 3.Key points for operation:To be formulated above after mixing, sterilizing be packaged to get.

Claims (10)

1. the recovery method that canned sardines processes fish protein in fish soup waste liquid, it is characterised in that:Canned sardines processes fish Soup waste liquid carries out degreasing with n-hexane, and hydrochloric acid tune pH value is added in the fish soup after degreasing makes it generate precipitating, then will precipitating Centrifuge separation, drying are to get fish protein.
2. the recovery method of fish protein, feature exist in canned sardines processing fish soup waste liquid according to claim 1 In:Specific step is as follows:
(1)Canned sardines processes the n-hexane extraction degreasing of 2-2.5 times of quality of fish soup waste liquid, to upper solution extracted N-hexane is recycled, it is stand-by to collect lower aqueous solution extracted;
(2)The pH value that lower aqueous solution is adjusted with HCl solution is 3.5-4.5, stands 20-30 min, is then centrifuged for, discards upper layer Solution collects sediment;
(3)Sediment is freeze-dried to get fish protein.
3. the recovery method of fish protein, feature exist in canned sardines processing fish soup waste liquid according to claim 2 In:The pH value for adjusting lower aqueous solution is 4.0.
4. the recovery method of fish protein, feature exist in canned sardines processing fish soup waste liquid according to claim 2 In:The molar concentration of the HCl solution is 0.1-0.15 mol/L.
5. the recovery method of fish protein, feature exist in canned sardines processing fish soup waste liquid according to claim 2 In:The centrifugal condition is 4°C, 6500-7500 r/min are centrifuged 20-25 min.
6. the recovery method of fish protein, feature exist in canned sardines processing fish soup waste liquid according to claim 2 In:The temperature of the vacuum freeze drying is -78°C, vacuum degree<5Pa.
7. the recovery method of fish protein, feature exist in canned sardines processing fish soup waste liquid according to claim 2 In:The purity of gained protein is 40 ~ 50%.
8. the fish protein that -7 any recovery methods obtain according to claim 1.
9. application of the fish protein as claimed in claim 8 in food.
10. fish protein according to claim 9 is applied in food processing, it is characterised in that:Fish protein is processed At fish protein powder;Or it is made an addition to after crushing fish protein in biscuit, bread, cake, noodles or flavouring.
CN201810773307.3A 2018-07-14 2018-07-14 Canned sardines processes the recovery method of fish protein and its application in fish soup waste liquid Pending CN108840902A (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101948533A (en) * 2010-09-21 2011-01-19 苏州大学 Preparation method of fish skin collagen
CN102657338A (en) * 2012-04-19 2012-09-12 天津科技大学 Method for processing red sea bream collagen polypeptide-chromium (III) chelate
CN102757491B (en) * 2012-07-11 2013-11-20 集美大学 Method for recycling water-soluble proteins in minced fish rinsing water
CN103571902A (en) * 2013-10-15 2014-02-12 江苏科技大学 Leiocassis longirostris skin collagen antioxidant peptide and preparation method and application thereof
CN103849671A (en) * 2014-03-25 2014-06-11 福州大学 Method of preparing antioxidative peptide by enzymolysis of byproducts in mackerel can processing
CN104489602A (en) * 2014-11-24 2015-04-08 威海市桢昊生物技术有限公司 A method for preparing seafood seasonings using fishmeal processing wastewater

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101948533A (en) * 2010-09-21 2011-01-19 苏州大学 Preparation method of fish skin collagen
CN102657338A (en) * 2012-04-19 2012-09-12 天津科技大学 Method for processing red sea bream collagen polypeptide-chromium (III) chelate
CN102757491B (en) * 2012-07-11 2013-11-20 集美大学 Method for recycling water-soluble proteins in minced fish rinsing water
CN103571902A (en) * 2013-10-15 2014-02-12 江苏科技大学 Leiocassis longirostris skin collagen antioxidant peptide and preparation method and application thereof
CN103849671A (en) * 2014-03-25 2014-06-11 福州大学 Method of preparing antioxidative peptide by enzymolysis of byproducts in mackerel can processing
CN104489602A (en) * 2014-11-24 2015-04-08 威海市桢昊生物技术有限公司 A method for preparing seafood seasonings using fishmeal processing wastewater

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Application publication date: 20181120