CN108814764A - A kind of device and its application method of experimental animal retention enema - Google Patents
A kind of device and its application method of experimental animal retention enema Download PDFInfo
- Publication number
- CN108814764A CN108814764A CN201810524035.3A CN201810524035A CN108814764A CN 108814764 A CN108814764 A CN 108814764A CN 201810524035 A CN201810524035 A CN 201810524035A CN 108814764 A CN108814764 A CN 108814764A
- Authority
- CN
- China
- Prior art keywords
- fixing pipe
- experimental animal
- fixed
- syringe
- beaker
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000010171 animal model Methods 0.000 title claims abstract description 52
- 241000792859 Enema Species 0.000 title claims abstract description 36
- 239000007920 enema Substances 0.000 title claims abstract description 36
- 229940095399 enema Drugs 0.000 title claims abstract description 36
- 230000014759 maintenance of location Effects 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 17
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims abstract description 80
- 238000012360 testing method Methods 0.000 claims abstract description 48
- 229910052742 iron Inorganic materials 0.000 claims abstract description 40
- 210000000436 anus Anatomy 0.000 claims abstract description 24
- 230000010412 perfusion Effects 0.000 claims abstract description 18
- 241001465754 Metazoa Species 0.000 claims abstract description 13
- 230000000694 effects Effects 0.000 claims abstract description 8
- 239000003814 drug Substances 0.000 claims description 32
- 229940079593 drug Drugs 0.000 claims description 31
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 239000010425 asbestos Substances 0.000 claims description 13
- 229910052895 riebeckite Inorganic materials 0.000 claims description 13
- 239000008236 heating water Substances 0.000 claims description 12
- 238000002474 experimental method Methods 0.000 claims description 11
- 238000002347 injection Methods 0.000 claims description 8
- 239000007924 injection Substances 0.000 claims description 8
- 238000001802 infusion Methods 0.000 claims description 7
- 239000000843 powder Substances 0.000 claims description 6
- 239000005662 Paraffin oil Substances 0.000 claims description 5
- 210000002429 large intestine Anatomy 0.000 claims description 4
- 238000003780 insertion Methods 0.000 claims description 3
- 230000037431 insertion Effects 0.000 claims description 3
- 238000002627 tracheal intubation Methods 0.000 claims description 3
- 239000004575 stone Substances 0.000 claims description 2
- 229920000742 Cotton Polymers 0.000 claims 1
- 238000002560 therapeutic procedure Methods 0.000 abstract description 6
- 230000006641 stabilisation Effects 0.000 abstract description 3
- 238000011105 stabilization Methods 0.000 abstract description 3
- 230000007547 defect Effects 0.000 abstract description 2
- 241000699666 Mus <mouse, genus> Species 0.000 description 33
- 241000283973 Oryctolagus cuniculus Species 0.000 description 9
- 239000000243 solution Substances 0.000 description 4
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 210000000936 intestine Anatomy 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241001093951 Ailanthus altissima Species 0.000 description 1
- 244000144730 Amygdalus persica Species 0.000 description 1
- 235000010894 Artemisia argyi Nutrition 0.000 description 1
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241000581650 Ivesia Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 244000082204 Phyllostachys viridis Species 0.000 description 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- 240000003186 Stachytarpheta cayennensis Species 0.000 description 1
- 235000009233 Stachytarpheta cayennensis Nutrition 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 244000089698 Zanthoxylum simulans Species 0.000 description 1
- 244000126002 Ziziphus vulgaris Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 244000030166 artemisia Species 0.000 description 1
- 239000011425 bamboo Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 239000008159 sesame oil Substances 0.000 description 1
- 235000011803 sesame oil Nutrition 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 235000019640 taste Nutrition 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 235000021419 vinegar Nutrition 0.000 description 1
- 239000000052 vinegar Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 239000001993 wax Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M31/00—Devices for introducing or retaining media, e.g. remedies, in cavities of the body
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M2250/00—Specially adapted for animals
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Heart & Thoracic Surgery (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Anesthesiology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Infusion, Injection, And Reservoir Apparatuses (AREA)
Abstract
A kind of device and its application method of experimental animal retention enema, belong to the device and its application method of a kind of enema therapy.For the defect of existing enema therapy, provide a kind of result stabilization, proper temperature, time-saving and efficiency, economical and practical animal retention enema method.In the present invention, No. two iron stands and No. three iron stand collective effects successively clamp No.1 fixed plate, No. two fixed plates and No. three fixed plates from bottom to top, fixing pipe is fixed in the circular socket holes of No.1 fixed plate, fixing pipe pipe lid covers fixed bottom of the tube, experimental animal is placed in fixing pipe, in the anus of one end loading test animal of the blindenea, the other end and syringe connect, the syringe is fixed in the circular socket holes in No. three fixed plates, one end of the perfusion tube is connect with syringe, and the other end is inserted into test tube.The present invention is mainly suitable for the administrations of retention enema simultaneously of more experimental animals.
Description
Technical field
The invention belongs to a kind of device of enema therapy and its application methods.
Background technique
Device at present dedicated for the administration of experimental animal bowel lavage is seldom, can not achieve more experimental animals while retaining filling
Enteral administration.Enema therapy is that pharmaceutical preparation is poured into big enteral from anus, to treat a kind of method of disease.It is filled using drug
Intestines treat disease, and China has long history, can trace back to the Eastern Han Dynasty earliest.?《Huatuo highly skilled doctor secretly hands down》In one book,
Also it describes name doctor Huatuo and treats all sides of constipation with enema therapy.As " pig gall one has, and takes juice people's vinegar a little, takes three or four cun of persons of bamboo,
Partly to receive in anus, juice is filled into people, a food hectare, when just.Again with Chinese prickly ash, fermented soya bean decocting bowel lavage.Again with tree of heaven root juice, sesame oil, Gan Dian
Three tastes close filling, also under.Again with the white skin of peach, kuh-seng, Chinese mugwort, jujube decoct fill, also under." simultaneously, it should be infused when also emphasizing application bowel lavage art
Meaning fluid temperature, it is indicated that：" Shi Shushi, medicine palpus tepor, does not overheat, is not subcooled." illustrate enema fluid proper temperature, just it is beneficial to increase
Strong curative effect.Drug temperature is changed, to reach suitable bowel lavage temperature, conventional efficient by water-bath temperature adjustment with reference to Chinese medical book
Height, Protection animal rationally utilize resource, avoid waste caused by many experiments.
It is therefore desirable to a kind of result stabilization, proper temperature, time-saving and efficiency, economical and practical enteroclysm device and its use
Method.
Summary of the invention
The present invention for existing enema therapy experimental result is unstable, temperature is not easy to control, time-consuming and laborious, low efficiency,
Economical and practical defect provides a kind of result stabilization, proper temperature, time-saving and efficiency, economical and practical experimental animal retain and fill
The method of intestines.
A kind of technical solution of the device of experimental animal retention enema according to the present invention is as follows：
A kind of device of experimental animal retention enema according to the present invention, it includes No.1 iron stand, beaker, asbestos
Net, beaker bracket, test tube and alcolhol burner, the No.1 iron stand are placed on the side of beaker bracket, and the asbestos gauge is put
It sets on beaker bracket, the alcolhol burner is placed in beaker bracket lower part, and the beaker is placed on asbestos gauge, and in beaker
It is filled with water, the No.1 iron stand grips test tube, is placed in the lower part of test tube in beaker below the water surface；
It further includes No. two iron stands, No. three iron stands, No.1 fixed plate, No. two fixed plates, No. three fixed plates, infusion
Pipe, syringe, blindenea, fixing pipe and fixing pipe pipe lid, No. two iron stands and No. three iron stand collective effects by
Under to upper successively clamp No.1 fixed plate, No. two fixed plates and No. three fixed plates, and No.1 fixed plate, No. two fixed plates and No. three
Fixed plate offers the corresponding circular socket holes in upper and lower position, and the fixing pipe is fixed on the circular socket holes of No.1 fixed plate
In, the fixing pipe pipe lid covers fixed bottom of the tube, and experimental animal is placed in fixing pipe, one end of the blindenea
In the anus of loading test animal, the other end and syringe connect, the syringe is fixed on No. two fixed plates and No. three are solid
In circular socket holes on fixed board, one end of the perfusion tube is connect with syringe, and the other end is inserted into test tube.
Further：Place thermometer in the test tube, the size of the fixing pipe is according to the size of experimental animal
Setting.
Further：No.1 flow regulator, No. two flow regulators and drop pot are additionally provided in the middle part of the perfusion tube,
The drop pot is located between No.1 flow regulator and No. two flow regulators.
Further：The pipe shaft of the blindenea and the tail of experimental animal are fixed.
Further：The fixing pipe two sides offer multiple bar shaped jacks up and down, and fixing pipe inserted sheet is dynamic according to experiment
The height of object, which is adjusted, is inserted into corresponding bar shaped jack；The periphery of the fixing pipe pipe lid is equipped with round vent, and described consolidates
Determine to offer No.1 hole and No. two holes on pipe inserted sheet.
A kind of application method based on the experimental animal retention enema device, it includes the following steps：
Step 1: experimental animal is fitted into fixing pipe, fixing pipe inserted sheet is inserted by bar shaped according to the height of experimental animal
Jack, for fixing experimental animal；The tail of experimental animal is passed through No. two holes on fixing pipe inserted sheet；By the one of blindenea
End picks paraffin oil, lubricates one end of blindenea, and blindenea is passed through the No.1 hole on fixing pipe inserted sheet and is slowly inserted into reality
Test the anus of animal；The tail of experimental animal is fixed on the pipe shaft of blindenea with buckle；
Step 2: being inverted fixing pipe, fixing pipe is fixed in the circular socket holes of No.1 fixed plate；
Step 3: syringe is inserted into the circular socket holes of No. two fixed plates and No. three fixed plates, by the another of blindenea
One end is connect with syringe；
Step 4: the built-in water of beaker, is used for heating water bath drug, the test tube of powder charge is fixed with No.1 iron stand and is placed in burning
In cup, and thermometer is placed in test tube；
Step 5: one end of the perfusion tube with drop pot is inserted into test tube, the other end connects syringe, closing No.1 stream
Fast adjuster and No. two flow regulators；
Step 6: extinguishing alcolhol burner to heating water bath to predetermined temperature, No.1 flow regulator is opened, max-flow is adjusted to
Speed, hand pinch drop pot, make drug infusion into drop pot, close No.1 flow regulator；
Step 7: opening No. two flow regulators, it is adjusted to maximum flow rate, hand pinches drop pot, flows into drug in syringe, closes
Close No. two flow regulators；
Step 8: being slowly advanced the piston of syringe, drug is poured into the large intestine of experimental animal, root by experimental animal anus
According to the dose for needing to adjust injection, fixing pipe stands half an hour, and retention enema terminates.
Further：In step 6, the predetermined temperature is set according to specific drug required temperature.
Further：In step 8, animal sets the dose of injection according to specific experiments.
A kind of device of experimental animal retention enema according to the present invention and its beneficial effect of application method are：
The device and its application method of a kind of experimental animal retention enema according to the present invention are easy to operate, formula is clever
Work, idicatio is wide, drug absorption is fast, effective concentration is high, therapeutic effect is good, experimental animal no pain, and the present invention also has following
Advantage：
1, result is stablized：Experimental method is mature, has been applied in scientific experiment, and success rate is high, avoids repeating to test, to reality
It is small to test animal injury, Protection animal, and can be used for that experimental period is long, need to carry out experimental animal the experiment of retention enema
In the middle；
2, proper temperature：Experiment heats close to human body drug with water bath heating to preferably simulate clinic
, can be as needed according to medication difference for 37 degrees Celsius of temperature, different temperature is adjusted by immersion method, is carried out different
Scientific research；
3, time-saving and efficiency：In animal experiments, the every group of suggestion of inbred strais experimental animal is 6 using number of elements, present invention behaviour
Make simple, it can be achieved that more simultaneously experimental animals carry out bowel lavage, saving experimental period simultaneously；
4, economical and practical：Experiment equipment is at low cost, saves research funding, to clinic common equipment disposable transfusion device, one
Secondary property syringe, disposable blindenea are transformed, and materials are easy, and concentrate disinfection, and Protection animal is less prone to sense
Dye.
Detailed description of the invention
Fig. 1 is the schematic device of experimental animal retention enema；
Fig. 2 is the partial enlarged view of Fig. 1.
Specific embodiment
Below with reference to embodiment, the following further describes the technical solution of the present invention, and however, it is not limited to this, all right
Technical solution of the present invention is modified or replaced equivalently, and without departing from the spirit and scope of the technical solution of the present invention, should all be contained
Lid is within the protection scope of the present invention.
Embodiment 1
Illustrate the present embodiment in conjunction with Fig. 1 and Fig. 2, in the present embodiment, sets experimental animal as mouse, it is involved in the present invention
A kind of experimental animal retention enema device, it includes No.1 iron stand 1, beaker 2, asbestos gauge 3, beaker bracket 4, test tube 5
And alcolhol burner, the No.1 iron stand 1 are placed on the side of beaker bracket 4, the asbestos gauge 3 is placed on beaker bracket 4
On, the alcolhol burner is placed in 4 lower part of beaker bracket, and the beaker 2 is placed on asbestos gauge 3, and is filled with water in beaker 2, institute
The No.1 iron stand 1 stated grips test tube 5, is placed in the lower part of test tube 5 in beaker 2 below the water surface；
It further includes 8, No. three iron stands 9 of No. two iron stands, the fixed plate of fixed plate 11, three of No.1 fixed plate 10, two
12, perfusion tube 13, syringe 14, blindenea 15, fixing pipe 16 and fixing pipe pipe lid 17, No. two iron stands 8 and three
Number 9 collective effect of iron stand successively clamps 10, No. two fixed plates 11 of No.1 fixed plate and No. three fixed plates 12, and one from bottom to top
Number 10, No. two fixed plates 11 of fixed plate and No. three fixed plates 12 offer the corresponding circular socket holes in upper and lower position, and described consolidates
Determine pipe 16 to be fixed in the circular socket holes of No.1 fixed plate 10, the fixing pipe pipe lid 17 covers 16 bottom of fixing pipe, mouse
It being placed in fixing pipe 16, one end of the blindenea 15 is inserted into the anus of mouse, the other end and syringe 14 connect,
The syringe 14 is fixed in the circular socket holes in No. two fixed plates 11 and No. three fixed plates 12, the perfusion tube 13
One end is connect with syringe 14, and the other end is inserted into test tube 5.
Further：Place thermometer 6 in the test tube 5, the size of the fixing pipe 16 is according to the size of mouse
Setting.
Further：The middle part of the perfusion tube 13 is additionally provided with 19, No. two flow regulators 20 of No.1 flow regulator
With drop pot 21, the drop pot 21 is located between No.1 flow regulator 19 and No. two flow regulators 20.
Further：The pipe shaft of blindenea 15 and the tail of mouse is fixed.
Further：16 two sides of fixing pipe offer multiple bar shaped jacks up and down, and fixing pipe inserted sheet 18 is according to small
The height of mouse, which is adjusted, is inserted into corresponding bar shaped jack；The periphery of the fixing pipe pipe lid 17 is equipped with round vent, described
No.1 hole and No. two holes are offered on fixing pipe inserted sheet 18.
A kind of application method based on the experimental animal retention enema device, it includes the following steps：
Step 1: mouse is fitted into fixing pipe 16, fixing pipe inserted sheet 18 is inserted by bar shaped jack according to the height of mouse,
For fixing mouse；The tail of mouse is passed through No. two holes on fixing pipe inserted sheet 18；One end of blindenea 15 is picked into stone
Wax oil lubricates one end of blindenea 15, and blindenea 15 is passed through the No.1 hole on fixing pipe inserted sheet 18 and is slowly inserted into mouse
Anus；The tail of mouse is fixed on the pipe shaft of blindenea 15 with buckle；
Step 2: being inverted fixing pipe 16, fixing pipe is fixed in the circular socket holes of No.1 fixed plate 10；
Step 3: syringe 14 is inserted into the circular socket holes of No. two fixed plates 11 and No. three fixed plates 12, anus is inserted
The other end of pipe 15 is connect with syringe 14；
Step 4: the built-in water of beaker 2, is used for heating water bath drug, the fixation of No.1 iron stand 1 of test tube 5 of powder charge is set
It is placed in test tube 5 in beaker 2, and by thermometer 6；
Step 5: one end of the perfusion tube 13 with drop pot 21 is inserted into test tube 5, the other end connects syringe 14, pass
Close No.1 flow regulator 19 and No. two flow regulators 20；
Step 6: extinguishing alcolhol burner to heating water bath to predetermined temperature, No.1 flow regulator 19 is opened, maximum is adjusted to
Flow velocity, hand pinch drop pot 21, make drug infusion into drop pot, close No.1 flow regulator 19；
Step 7: opening No. two flow regulators 20, it is adjusted to maximum flow rate, hand pinches drop pot 21, drug is made to flow into syringe
In 14, No. two flow regulators 20 are closed；
Step 8: being slowly advanced the piston of syringe 14, drug is poured into the large intestine of mouse by mouse anus, as needed
The dose of injection is adjusted, fixing pipe stands half an hour, and retention enema terminates.
It includes No.1 iron stand 1, beaker 2, asbestos gauge 3, beaker bracket 4, test tube 5 and alcolhol burner, the No.1 iron
Pallet 1 is placed on the side of beaker bracket 4, and the asbestos gauge 3 is placed on beaker bracket 4, and the alcolhol burner is placed in burning
4 lower part of cup bracket, the beaker 2 is placed on asbestos gauge 3, and is filled with water in beaker 2, and the clamping of No.1 iron stand 1 is solid
Determine test tube 5, is placed in the lower part of test tube 5 in beaker 2 below the water surface；
It further includes 8, No. three iron stands 9 of No. two iron stands, the fixed plate of fixed plate 11, three of No.1 fixed plate 10, two
12, perfusion tube 13, syringe 14, blindenea 15, fixing pipe 16 and fixing pipe pipe lid 17, No. two iron stands 8 and three
Number 9 collective effect of iron stand successively clamps 10, No. two fixed plates 11 of No.1 fixed plate and No. three fixed plates 12, and one from bottom to top
Number 10, No. two fixed plates 11 of fixed plate and No. three fixed plates 12 offer the corresponding circular socket holes in upper and lower position, and described consolidates
Determine pipe 16 to be fixed in the circular socket holes of No.1 fixed plate 10, the fixing pipe pipe lid 17 covers 16 bottom of fixing pipe, mouse
It being placed in fixing pipe 16, one end of the blindenea 15 is inserted into the anus of mouse, the other end and syringe 14 connect,
The syringe 14 is fixed in the circular socket holes in No. two fixed plates 11 and No. three fixed plates 12, the perfusion tube 13
One end is connect with syringe 14, and the other end is inserted into test tube 5.
More specifically：Thermometer 6 is placed in the test tube 5.
More specifically：The middle part of the perfusion tube 13 is additionally provided with 19, No. two flow regulators of No.1 flow regulator
20 are located between No.1 flow regulator 19 and No. two flow regulators 20 with drop pot 21, the drop pot 21.
More specifically：The pipe shaft of blindenea 15 and the tail of mouse is fixed.
More specifically：16 two sides of fixing pipe offer multiple bar shaped jacks, 18 basis of fixing pipe inserted sheet up and down
The height of mouse, which is adjusted, is inserted into corresponding bar shaped jack；The periphery of the fixing pipe pipe lid 17 is equipped with round vent.
A kind of application method of mouse retention enema device, it includes the following steps：
Step 1: mouse is fitted into fixing pipe 16, the tail of mouse is passed through the bar shaped jack on 16 pipe shaft of fixing pipe；
One end of blindenea 15 is picked into paraffin oil, lubricates one end of blindenea 15, blindenea 15 is passed through into bar shaped jack and is delayed
It is slowly inserted 3-4 centimetres of anus of mouse；The tail of mouse is fixed on the pipe shaft of blindenea 15 with buckle；
Step 2: fixing pipe inserted sheet 18 is inserted into bar shaped jack according to the height of mouse, for fixing mouse；It is inverted and fixes
Fixing pipe is fixed in the circular socket holes of No.1 fixed plate 10 by pipe 16；
Step 3: syringe 14 is inserted into the circular socket holes of No. two fixed plates 11 and No. three fixed plates 12, anus is inserted
The other end of pipe 15 is connect with syringe 14；
Step 4: the built-in water of beaker 2, is used for heating water bath drug, the fixation of No.1 iron stand 1 of test tube 5 of powder charge is set
It is placed in test tube 5 in beaker 2, and by thermometer 6；
Step 5: one end of the perfusion tube 13 with drop pot 21 is inserted into test tube 5, the other end connects syringe 14, pass
Close No.1 flow regulator 19 and No. two flow regulators 20；
Step 6: extinguishing alcolhol burner to heating water bath to predetermined temperature, No.1 flow regulator 19 is opened, maximum is adjusted to
Flow velocity, hand pinch drop pot 21, make drug infusion into drop pot, close No.1 flow regulator 19；
Step 7: opening No. two flow regulators 20, it is adjusted to maximum flow rate, hand pinches drop pot 21, drug is made to flow into syringe
In 14, No. two flow regulators 20 are closed；
Step 8: being slowly advanced the piston of syringe 14, drug is poured into the large intestine of mouse by mouse anus, as needed
The dose of injection is adjusted, fixing pipe stands half an hour, and retention enema terminates.
More specifically：In step 6, the predetermined temperature is 37 DEG C.
More specifically：In step 8, the dose of injection is 0.2-0.3ml.
Mouse is fitted into fixing pipe 16, mousetail is passed through the fixation bar shaped jack of fixing pipe inserted sheet 18, after by anus
Plug one end of door intubation 15 picks paraffin oil, and lubricated surface is suitable blindenea 15 with blindenea 15 by anus jack
Mouse anus be slowly inserted into 3-4cm, with fixed rat-tail and mouse blindenea 15 is buckled, according to the fixed mouse of mouse difference height
Fixing pipe inserted sheet 18 is inserted into fixing pipe corresponding position by body, and it is fixed with No.1 fixed plate 10 to be inverted fixing pipe 16 for fixed mouse；
No. two iron stands 8 and No. three fixed 10, No. two fixed plates 11 of No.1 fixed plate of 9 collective effect of iron stand and No. three fixed plates 12,
1ml syringe is inserted into the circular socket holes of No. two fixed plates and No. three fixed plates in order, and by blindenea 15 and syringe
14 connections；Beaker 2 is placed on the beaker bracket 4 for being lined with asbestos gauge 3, one alcolhol burner of lower placement of beaker bracket 4, in beaker 4
Pure water is filled for the drug in heating water bath test tube 5, the fixation of No.1 iron stand 1 of test tube 5 of powder charge is placed in beaker 2,
And one piece of thermometer 6 is placed in test tube 5, one end insertion test tube 5, other end connection injection the perfusion tube 13 with drop pot 21
Device 14 closes No.1 flow regulator 19 and No. two flow regulators 20, and it is Celsius that heating water bath drug to thermometer 6 is shown as 37
Degree extinguishes alcolhol burner, opens No.1 flow regulator, is transferred to maximum flow rate, and hand pinches drop pot 21, makes in drug infusion drop pot 21,
No.1 flow regulator 19 is closed, No. two flow regulators 20 are opened, is transferred to maximum flow rate, hand pinches drop pot 21, and drug flows into note
In emitter 14, No. two flow regulators 20 are closed, 14 piston of syringe is slowly advanced and carries out bowel lavage, inject 0.2ml- as needed
0.3ml, fixing pipe stand 30min and complete retention enema.
Embodiment 2
Illustrate the present embodiment in conjunction with Fig. 1 and Fig. 2, in the present embodiment, sets experimental animal as rabbit, rabbit is packed into solid
Determine in pipe 16, rabbit tail pass through fixing pipe inserted sheet 18 fixation bar shaped jack, after plug one end of blindenea 15 is stained with
Paraffin oil is taken, blindenea 15 is slowly inserted into 7- along rabbit anus with blindenea 15 by anus jack by lubricated surface
9cm, according to the fixed rabbit body of rabbit difference height, inserts fixing pipe inserted sheet 18 with fixed tail and rabbit blindenea 15 is buckled
Enter fixing pipe corresponding position, fixed rabbit, the height 20-40cm of average adult rabbit, bust 10-20cm, the left side weight 2-3kg
The right side, the height 10-15cm of experiment mice, bust 5-7cm, weight 20-30g or so, according to the different height bust of experimental animal
Weight designs the fixing pipe of different size and fixes inserted sheet；It is fixed with No.1 fixed plate 10 to be inverted fixing pipe 16；No. two iron stands
8 and fixed 10, No. two fixed plates 11 of No.1 fixed plate of No. three 9 collective effects of iron stand and No. three fixed plates 12,10ml is injected
Device is inserted into the circular socket holes of No. two fixed plates and No. three fixed plates in order, and blindenea 15 is connect with syringe 14；It will
Beaker 2 is placed on the beaker bracket 4 for being lined with asbestos gauge 3, and beaker bracket 4 is lower to place an alcolhol burner, and the built-in pure water of beaker 4 is used
Drug in heating water bath test tube 5 is placed in the fixation of No.1 iron stand 1 of test tube 5 of powder charge in beaker 2, and in test tube 5
One piece of thermometer 6 is placed, one end insertion test tube 5, the other end of the perfusion tube 13 with drop pot 21 are connected syringe 14, close one
Number flow regulator 19 and No. two flow regulators 20, heating water bath drug to thermometer 6 are shown as 38.5 degrees Celsius, extinguish wine
Smart lamp opens No.1 flow regulator, is transferred to maximum flow rate, and hand pinches drop pot 21, makes to close No.1 stream in drug infusion drop pot 21
Fast adjuster 19 opens No. two flow regulators 20, is transferred to maximum flow rate, and hand pinches drop pot 21, and drug flows into syringe 14, closes
No. two flow regulators 20 are closed, 14 piston of syringe is slowly advanced and carries out bowel lavage, inject 10ml-20ml, fixing pipe as needed
It stands 30min and completes retention enema.
Claims (8)
1. a kind of device of experimental animal retention enema, it includes No.1 iron stand (1), beaker (2), asbestos gauge (3), beaker branch
Frame (4), test tube (5) and alcolhol burner, the No.1 iron stand (1) are placed on the side of beaker bracket (4), the asbestos gauge
(3) it is placed on beaker bracket (4), the alcolhol burner is placed in beaker bracket (4) lower part, and the beaker (2) is placed on stone
It on cotton net (3), and is filled with water in beaker (2), the No.1 iron stand (1) grips test tube (5), makes the lower part of test tube (5)
It is placed in beaker (2) interior water surface or less；
It is characterized in that, it further includes No. two iron stands (8), No. three iron stands (9), No.1 fixed plate (10), No. two fixed plates
(11), No. three fixed plates (12), perfusion tube (13), syringe (14), blindenea (15), fixing pipe (16) and fixing pipe pipe lid
(17), No. two iron stands (8) and No. three iron stand (9) collective effects successively clamp No.1 fixed plate from bottom to top
(10), No. two fixed plates (11) and No. three fixed plates (12), and No.1 fixed plate (10), No. two fixed plates (11) and No. three fixations
Plate (12) offers the corresponding circular socket holes in upper and lower position, and the fixing pipe (16) is fixed on the circle of No.1 fixed plate (10)
In shape jack, the fixing pipe pipe lid (17) covers fixing pipe (16) bottom, and experimental animal is placed in fixing pipe (16), institute
In the anus of one end loading test animal for the blindenea (15) stated, the other end and syringe (14) connect, the injection
Device (14) is fixed in the circular socket holes on No. two fixed plates (11) and No. three fixed plates (12), and the one of the perfusion tube (13)
End is connect with syringe (14), and the other end is inserted into test tube (5).
2. a kind of device of experimental animal retention enema according to claim 1, which is characterized in that the test tube (5)
The size of interior placement thermometer (6), the fixing pipe (16) is set according to the size of experimental animal.
3. a kind of device of experimental animal retention enema according to claim 2, which is characterized in that the perfusion tube
(13) No.1 flow regulator (19), No. two flow regulators (20) and drop pot (21), the drop pot are additionally provided in the middle part of
(21) between No.1 flow regulator (19) and No. two flow regulators (20).
4. a kind of device of experimental animal retention enema according to claim 3, which is characterized in that the blindenea
(15) tail of pipe shaft and experimental animal is fixed.
5. a kind of device of experimental animal retention enema according to claim 4, which is characterized in that the fixing pipe
(16) two sides offer multiple bar shaped jacks up and down, and fixing pipe inserted sheet (18) adjusts insertion accordingly according to the height of experimental animal
Bar shaped jack；The periphery of the fixing pipe pipe lid (17) is equipped with round vent, opens up on the fixing pipe inserted sheet (18)
There are No.1 hole and No. two holes.
6. a kind of application method based on a kind of experimental animal retention enema device described in claim 5, which is characterized in that it
Include the following steps：
Step 1: experimental animal is fitted into fixing pipe (16), fixing pipe inserted sheet (18) are inserted into according to the height of experimental animal
Bar shaped jack, for fixing experimental animal；The tail of experimental animal is passed through No. two holes on fixing pipe inserted sheet (18)；By anus
One end of intubation (15) picks paraffin oil, lubricates the one end of blindenea (15), and blindenea (15) are passed through fixing pipe inserted sheet
(18) anus of the slow loading test animal in No.1 hole on；The tail of experimental animal is fixed on blindenea (15) with buckle
Pipe shaft on；
Step 2: being inverted fixing pipe (16), fixing pipe is fixed in the circular socket holes of No.1 fixed plate (10)；
Step 3: syringe (14) are inserted into the circular socket holes of No. two fixed plates (11) and No. three fixed plates (12), by anus
The other end of intubation (15) is connect with syringe (14)；
Step 4: beaker (2) built-in water, is used for heating water bath drug, the test tube (5) powder charge is fixed with No.1 iron stand (1)
It is placed in beaker (2), and thermometer (6) is placed in test tube (5)；
Step 5: one end of the perfusion tube (13) with drop pot (21) is inserted into test tube (5), the other end connects syringe
(14), No.1 flow regulator (19) and No. two flow regulators (20) are closed；
Step 6: extinguishing alcolhol burner to heating water bath to predetermined temperature, opens No.1 flow regulator (19), be adjusted to max-flow
Speed, hand pinch drop pot (21), make drug infusion into drop pot, close No.1 flow regulator (19)；
Step 7: opening No. two flow regulators (20), it is adjusted to maximum flow rate, hand pinches drop pot (21), and drug is made to flow into syringe
(14) in, No. two flow regulators (20) are closed；
Step 8: being slowly advanced the piston of syringe (14), drug is poured into the large intestine of experimental animal, root by experimental animal anus
According to the dose for needing to adjust injection, fixing pipe stands half an hour, and retention enema terminates.
7. a kind of device of experimental animal retention enema according to claim 6, which is characterized in that in step 6, institute
The predetermined temperature stated is set according to specific drug required temperature.
8. a kind of device of experimental animal retention enema according to claim 6, which is characterized in that in step 8, note
Animal sets the dose entered according to specific experiments.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810524035.3A CN108814764B (en) | 2018-05-28 | 2018-05-28 | Device for retention enema of experimental animal and using method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810524035.3A CN108814764B (en) | 2018-05-28 | 2018-05-28 | Device for retention enema of experimental animal and using method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108814764A true CN108814764A (en) | 2018-11-16 |
| CN108814764B CN108814764B (en) | 2021-05-18 |
Family
ID=64146304
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810524035.3A Active CN108814764B (en) | 2018-05-28 | 2018-05-28 | Device for retention enema of experimental animal and using method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108814764B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112891013A (en) * | 2021-01-27 | 2021-06-04 | 中国人民解放军空军军医大学 | Device for injecting medicine into colorectal cavity of small rodent experimental animal |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101474441A (en) * | 2009-01-22 | 2009-07-08 | 湖州市农业科学研究院 | Combined injection machine for insect chrysalis |
| CN101637411A (en) * | 2009-08-12 | 2010-02-03 | 大连大学 | Tiny vein injection drug delivery system on body surface of experimental animal |
| CN201977963U (en) * | 2011-03-04 | 2011-09-21 | 施瑞客（天津）生物技术有限公司 | Continuously adjustable veterinary medicine administering apparatus |
| CN204016549U (en) * | 2014-08-25 | 2014-12-17 | 黑龙江农业经济职业学院 | A kind of domestic animal continuous medicine-filling device |
| CN206198091U (en) * | 2016-07-07 | 2017-05-31 | 黑龙江中医药大学 | Experiment mice sacculus irrigation stomach device |
| WO2018026937A1 (en) * | 2016-08-04 | 2018-02-08 | Charles R. Drew University Of Medicine And Science | Aerosol generation and exposure system |
| CN207085016U (en) * | 2017-02-28 | 2018-03-13 | 柳州市妇幼保健院 | A kind of bowel lavage injection device |
-
2018
- 2018-05-28 CN CN201810524035.3A patent/CN108814764B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101474441A (en) * | 2009-01-22 | 2009-07-08 | 湖州市农业科学研究院 | Combined injection machine for insect chrysalis |
| CN101637411A (en) * | 2009-08-12 | 2010-02-03 | 大连大学 | Tiny vein injection drug delivery system on body surface of experimental animal |
| CN201977963U (en) * | 2011-03-04 | 2011-09-21 | 施瑞客（天津）生物技术有限公司 | Continuously adjustable veterinary medicine administering apparatus |
| CN204016549U (en) * | 2014-08-25 | 2014-12-17 | 黑龙江农业经济职业学院 | A kind of domestic animal continuous medicine-filling device |
| CN206198091U (en) * | 2016-07-07 | 2017-05-31 | 黑龙江中医药大学 | Experiment mice sacculus irrigation stomach device |
| WO2018026937A1 (en) * | 2016-08-04 | 2018-02-08 | Charles R. Drew University Of Medicine And Science | Aerosol generation and exposure system |
| CN207085016U (en) * | 2017-02-28 | 2018-03-13 | 柳州市妇幼保健院 | A kind of bowel lavage injection device |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112891013A (en) * | 2021-01-27 | 2021-06-04 | 中国人民解放军空军军医大学 | Device for injecting medicine into colorectal cavity of small rodent experimental animal |
| CN112891013B (en) * | 2021-01-27 | 2023-08-25 | 中国人民解放军空军军医大学 | A device for drug perfusion in the colorectum of small rodent experimental animals |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108814764B (en) | 2021-05-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN208525537U (en) | A kind of automatic enteroclysm device | |
| CN209519187U (en) | A kind of Internal Medicine-Oncology drug delivery device | |
| CN108814764A (en) | A kind of device and its application method of experimental animal retention enema | |
| CN104027442B (en) | A kind of Chinese medicine preparation, preparation method and the method for use Chinese medicine preparation cultivation broiler chicken used in green no drug residue broiler breeding | |
| CN205850010U (en) | A kind of reclining posture three-hypers physical therapy cabin | |
| CN209220920U (en) | A kind of fumigator for Chinese medicine | |
| CN102697842A (en) | Lactation promotion preparation and preparation process | |
| CN2920232Y (en) | Chinese herbal medicines fumigating physical therapeutical health care device | |
| CN201603222U (en) | Oxygenating and atomizing inhalator for treating pneumoconiosis | |
| CN204766781U (en) | Constipation therapeutic instrument | |
| CN204147336U (en) | The disposable colon clysis drug-supplying system improved | |
| CN201328924Y (en) | Chinese medicine fumigation treatment device for eye disease | |
| CN208770525U (en) | A gastroenterer for turtles | |
| CN205885791U (en) | Traditional chinese medical science steam detoxifies and rehabilitation appearance | |
| CN109248346A (en) | A kind of Cardiological cardiovascular disease adjuvant therapy device | |
| CN210228873U (en) | Novel gastroenterology catharsis ware | |
| CN204581938U (en) | Umbilicus fire therapeutic instrument | |
| CN202366135U (en) | Traditional Chinese medicine fumigation device | |
| CN201469831U (en) | Heating belt used for infusion tube | |
| CN103845208A (en) | Moxa-moxibustion box | |
| CN203227199U (en) | Cervical vertebra fumigation-moxibustion therapeutic instrument | |
| CN105147415B (en) | Milk cow vein Special remaining needle | |
| CN217960858U (en) | Medicine infusion device for chemotherapy of tumor patient | |
| CN214415242U (en) | Ironing moxibustion pot | |
| CN2780186Y (en) | Fumigation device for breast |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |