CN108679448A - Microfluid flow on-line control device and detection method - Google Patents

Microfluid flow on-line control device and detection method Download PDF

Info

Publication number
CN108679448A
CN108679448A CN201810559797.7A CN201810559797A CN108679448A CN 108679448 A CN108679448 A CN 108679448A CN 201810559797 A CN201810559797 A CN 201810559797A CN 108679448 A CN108679448 A CN 108679448A
Authority
CN
China
Prior art keywords
pressure
valve
microfluid
flow
pressure sensor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201810559797.7A
Other languages
Chinese (zh)
Other versions
CN108679448B (en
Inventor
曾文
魏欣彤
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Harbin Institute of Technology
Original Assignee
Harbin Institute of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harbin Institute of Technology filed Critical Harbin Institute of Technology
Priority to CN201810559797.7A priority Critical patent/CN108679448B/en
Publication of CN108679448A publication Critical patent/CN108679448A/en
Application granted granted Critical
Publication of CN108679448B publication Critical patent/CN108679448B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • FMECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F17STORING OR DISTRIBUTING GASES OR LIQUIDS
    • F17DPIPE-LINE SYSTEMS; PIPE-LINES
    • F17D3/00Arrangements for supervising or controlling working operations
    • F17D3/01Arrangements for supervising or controlling working operations for controlling, signalling, or supervising the conveyance of a product

Abstract

The invention discloses a kind of microfluid flow on-line control device and detection method, described device includes gas supply air source, pressure reducing valve, proportional pressure valve, the first liquid container, valve, first pressure sensor, second pressure sensor, microfluidic channel, micro-fluidic chip, second liquid container, weighing instrument and microprocessor.Pressure reducing valve is connected to the gas access of gas supply air source, proportional pressure valve;Proportional pressure valve is connected to the first liquid container;First liquid container is connected to valve;Valve is connected to microfluidic channel;Microfluidic channel is connected to micro-fluidic chip;Micro-fluidic chip and second liquid reservoir;First pressure sensor is arranged in the entrance of microfluidic channel, and second pressure sensor arrangement is in the outlet of microfluidic channel;Second liquid container is positioned on weighing instrument;Pressure sensor is connected with microprocessor;Microprocessor is connected with proportional pressure valve.The present invention can be achieved the online stable regulation of microfluid flow and be measured with accurate, has many advantages, such as that microfluid flow-rate adjustment is stable, detection speed is fast, accuracy of detection is high, to promote microfluidic system in the application study of interdisciplinary field, provides technical support.

Description

Microfluid flow on-line control device and detection method
Technical field
The present invention relates to a kind of microfluid flow on-line control device and detection methods, belong to microfluidic field.
Background technology
Syringe pump is the common flow-regulating components of microfluidic system.Syringe pump is driven using stepper motor, selectes injection The diameter of device and the rotating speed for setting stepper motor can calculate the ideal value of injection pump output flow.But using syringe pump tune There are shortcomings for the microfluid flow of section microfluidic system, such as:Reality output flow there are periodically pulsing, flows can not On-line checking, flow-rate adjustment speed is slow, precision is low etc..As microfluidic system is in the extensive of the fields such as chemistry, biology and medicine Using being based on pressure-actuated microfluid stream quantity regulating device, can replace syringe pump, realize the flow that microfluidic system is stablized It adjusts.Microfluid flow is adjusted using pressure actuated device, there is no periodical flow pulsation, flow-rate adjustment caused by pumping source are steady Calmly, speed is fast.However, as driving pressure increases, for microfluid flow there are nonlinear characteristic, the ideal of pressure actuated device is defeated Outflow is difficult to accurately calculate.Particularly, pressure actuated device and liquid drop microfluidic system are become one, due to drop body Product it is directly related with microfluid flow, during droplet formation, how on-line checking with adjust microfluid flow, for raising The stability of droplet formation and the control accuracy of droplet size promote liquid drop microfluidic system in the hair of interdisciplinary field Exhibition, it is very crucial.
Invention content
Adjust microfluidic system flow to solve current syringe pump, microfluid reality output flow there are periodically pulsing, And pressure actuated device adjusts microfluid flow, ideal output flow the problems such as there are nonlinear characteristics, the present invention provides A kind of microfluid flow on-line control device and detection method, the device and microfluidic system are become one, it can be achieved that The online stable regulation of microfluid flow is measured with accurate, to promote microfluidic system in the application study of interdisciplinary field, Technical support is provided.
The purpose of the present invention is what is be achieved through the following technical solutions:
Microfluid flow on-line control device of the present invention and detection method, it includes gas supply air source, pressure reducing valve, ratio pressure Valve, the first liquid container, valve, first pressure sensor, second pressure sensor, microfluidic channel, micro-fluidic chip, second Liquid container, weighing instrument and microprocessor.Wherein:
The gas access of the pressure reducing valve is connected to the compressed air outlet of gas supply air source, the gas vent and ratio pressure of pressure reducing valve The gas access of power valve is connected to;
The gas vent of the proportional pressure valve is connected to the gas access of the first liquid container, the liquid discharge of the first liquid container Mouth is connected to the entrance of valve;
The outlet of the valve is connected to the entrance of microfluidic channel, and the outlet of microfluidic channel and the entrance of micro-fluidic chip connect It is logical;
The outlet of the micro-fluidic chip is connected to the entrance of second liquid container;
The first pressure sensor is arranged in the entrance of microfluidic channel, and second pressure sensor arrangement is in microfluidic channel Outlet, second liquid container are positioned on weighing instrument;
The pressure signal of the signal output end and microprocessor of the first pressure sensor and second pressure sensor is defeated Enter end to be connected;
The control instruction signal output end of the microprocessor is connected with the control instruction signal input part of proportional pressure valve.
A kind of microfluid flow online test method using above-mentioned apparatus includes the following steps:
One, gas supply air source enters the first liquid container for filling liquid by pressure reducing valve and proportional pressure valve, holds in the first liquid Certain gas driving pressure is formed in device;
Two, as gas driving pressure increases, the liquid of the first liquid container flows into microfluid under air pressure driving by valve Channel is measured the entrance and exit pressure of microfluidic channel by first pressure sensor and second pressure sensor, obtained respectively The pressure difference at microfluidic channel both ends, and calculate the theoretical delivery of microfluid;
Three, microprocessor acquires the pressure signal of first pressure sensor and second pressure sensor in real time, while giving ratio pressure Power valve exports control instruction signal, changes the gas control pressure of proportional pressure valve, on-line control microfluid flow, and by weighing Instrument measures second liquid liquid in containers quality and changes with time rule, obtains the actual flow of microfluid, realizes microfluid The on-line checking of flow.
Compared with the prior art, the invention has the advantages that:
1)Microfluid flow-rate adjustment is stablized, and periodic flow pulsation is not present in reality output flow, using pressure actuated device Microfluid flow is adjusted, the dynamic regulation speed of flow can be improved, increase the adjustable range of flow.
2)Microfluid flow on-line checking precision is high, due to pressure sensor high certainty of measurement, and microfluid flow with it is micro- The pressure difference at fluid channel both ends has good linear characteristic, by measuring the pressure difference at microfluidic channel both ends, may be implemented micro- The online accurate detection of fluid flow.
3)Microfluid flow on-line checking speed is fast, due to pressure sensor fast response time, when microfluid flow changes When, by measuring the variation of microfluidic channel both ends pressure difference, the on-line quick detection of microfluid flow may be implemented.
4)Microfluid flow on-line control precision is high, and the pressure difference at microfluidic channel both ends is measured simultaneously by pressure sensor Microprocessor is fed back to, control signal is exported by microprocessor, changes the gas control pressure of proportional pressure valve, it can be achieved that miniflow The online accurate adjusting of body flow.
Description of the drawings
Fig. 1 is the fundamental diagram of microfluid flow on-line control device and detection method of the present invention;
Fig. 2 is the structure chart of two PTFE pipes of specific implementation mode;
Fig. 3 is the control block of three microfluid flow on-line control of specific implementation mode.
Specific implementation mode
Specific implementation mode one:Illustrate present embodiment with reference to Fig. 1 and Fig. 2, microfluid stream described in present embodiment On-line control device and detection method are measured, it is by gas supply air source 1, pressure reducing valve 2, proportional pressure valve 3, the first liquid container 4, valve 5, first pressure sensor 6, second pressure sensor 7, microfluidic channel 8, micro-fluidic chip 9, second liquid container 10, weigh Instrument 11 and microprocessor 12 are constituted.Wherein:
The gas access of the pressure reducing valve 2 with gas supply air source 1 compressed air outlet be connected to, the gas vent of pressure reducing valve 2 and than The gas access connection of example pressure valve 3;
The gas vent of the proportional pressure valve 3 is connected to the gas access of the first liquid container 4, the liquid of the first liquid container 4 Body outlet is connected to the entrance of valve 5;
The outlet of the valve 5 is connected to the entrance of microfluidic channel 8, the outlet of microfluidic channel 8 and entering for micro-fluidic chip 9 Mouth connection;
The outlet of the micro-fluidic chip 9 is connected to the entrance of second liquid container 10;
The first pressure sensor 6 is arranged in the entrance of microfluidic channel 8, and it is logical that second pressure sensor 7 is arranged in microfluid 8 outlets in road, second liquid container 10 are positioned on weighing instrument 12;
The first pressure sensor 6 and the signal output end of second pressure sensor 7 and the pressure of microprocessor 12 are believed Number input terminal is connected;
The control instruction signal output end of the microprocessor 12 is connected with the control instruction signal input part of proportional pressure valve 3.
The first pressure sensor 6 and second pressure sensor 7 measure the entrance and exit pressure of microfluidic channel 8 respectively Power obtains the pressure difference at 8 both ends of microfluidic channel and feeds back to microprocessor 12, exports control signal by microprocessor 12, changes The gas control pressure of proportional pressure valve 3 realizes the online accurate adjusting of microfluid flow.
Operation principle:
Present embodiment supplies compressed air by gas supply air source, and compressed air enters by pressure reducing valve and proportional pressure valve fills liquid First liquid container of body, forms certain gas driving pressure in the first liquid container, as gas driving pressure increases, The liquid of first liquid container flows into microfluidic channel under air pressure driving, by valve, and the first liquid is controlled by proportional pressure valve The gas driving pressure of body container adjusts the fluid flow for flowing into microfluidic channel by changing gas driving pressure.First pressure Force snesor, in the both ends of microfluidic channel, measures the entrance and exit of microfluidic channel respectively with second pressure sensor arrangement Pressure, while by the Pressure difference feedback at microfluidic channel both ends to microprocessor, based on microfluid flow and microfluidic channel both ends The linear relationship of pressure difference calculates the theoretical delivery of microfluid and compared with the setting flow of microfluid, is exported and controlled by microprocessor Signal processed changes the gas control pressure of proportional pressure valve, realizes the online accurate adjusting of microfluid flow.Meanwhile by weighing Instrument measures second liquid liquid in containers quality and changes with time rule, obtains the actual flow of microfluid, realizes microfluid The online accurate detection of flow.
Present embodiment can realize the online stable regulation of microfluid flow and accurate detection, to promote microfluidic system to exist The application study of interdisciplinary field, provides technical support.The supply gas pressure size for supplying air source 1 is 100~200 kPa, than The pressure regulation spectrum of example pressure valve 3 is 0.1~100 kPa, the maximum of first pressure sensor 6 and second pressure sensor 7 Range is 100 kPa, and measurement accuracy reaches 0.05 kPa, and the maximum range of weighing instrument is 500 g, minimum resolution 0.0001 g.Microfluid flowQAdjustable range be the mL/min of 0.002 mL/min~120.
Specific implementation mode two:Illustrate that present embodiment, present embodiment make specific implementation mode one with reference to Fig. 2 It further illustrates.To ensure that microfluid flow and the pressure difference at 8 both ends of microfluidic channel have linear relationship(Microfluid flowQ = Proportionality coefficientK× pressure differenceΔP), realize that the online accurate detection of microfluid flow, microfluidic channel 8 use PTFE circular tube structures, It is required that the length of pipeLWith internal diameterdThe ratio between be more than 20:1, and meet inside pipe the reynolds number Re of microfluidic flow< 1.0, microfluid is in laminar flow state.
In present embodiment, PTFE round tube inside diametersdVariation range be 0.1~1.0 mm, PTFE pipe lengthLVariation Ranging from 10.0~40.0 mm, according to microfluid flowQAdjustable range, obtain microfluidic channel both ends pressure differenceΔPVariation Ranging from 0.2~35.0 kPa.The on-line checking precision of microfluid flow reaches 0.1%.
Specific implementation mode three:Illustrate that present embodiment, present embodiment make specific implementation mode one with reference to Fig. 3 It further illustrates.To realize the online stable regulation of microfluid flow, first pressure sensor 6 and second pressure sensor 7 divide Not Ce Liang microfluidic channel 8 entrance and exit pressure, obtain the pressure difference at 8 both ends of microfluidic channel and feed back to microprocessor 12, control signal is exported by microprocessor 12, changes the gas control pressure of proportional pressure valve 3, on-line control microfluid flow.
In present embodiment, the on-line control time of microfluid flow is less than 0.5 s, the on-line control essence of microfluid flow Degree reaches 0.1%.

Claims (9)

1. a kind of microfluid flow on-line control device and detection method, it include gas supply air source, pressure reducing valve, proportional pressure valve, First liquid container, valve, first pressure sensor, second pressure sensor, microfluidic channel, micro-fluidic chip, the second liquid Body container, weighing instrument and microprocessor;
Wherein:
The gas access of the pressure reducing valve is connected to the compressed air outlet of gas supply air source, the gas vent and ratio pressure of pressure reducing valve The gas access of power valve is connected to;
The gas vent of the proportional pressure valve is connected to the gas access of the first liquid container, the liquid discharge of the first liquid container Mouth is connected to the entrance of valve;
The outlet of the valve is connected to the entrance of microfluidic channel, and the outlet of microfluidic channel and the entrance of micro-fluidic chip connect It is logical;
The outlet of the micro-fluidic chip is connected to the entrance of second liquid container;
The first pressure sensor is arranged in the entrance of microfluidic channel, and second pressure sensor arrangement is in microfluidic channel Outlet, second liquid container are positioned on weighing instrument;
The pressure signal of the signal output end and microprocessor of the first pressure sensor and second pressure sensor is defeated Enter end to be connected;
The control instruction signal output end of the microprocessor is connected with the control instruction signal input part of proportional pressure valve.
2. microfluid flow on-line control device according to claim 1, it is characterised in that the gas supply of the gas supply air source Pressure size is 100~200 kPa.
3. microfluid flow on-line control device according to claim 1, it is characterised in that the pressure tune of proportional pressure valve Adjusting range is 0.1~100 kPa.
4. microfluid flow on-line control device according to claim 1, it is characterised in that first pressure sensor and The maximum range of two pressure sensors is 100 kPa, and measurement accuracy reaches 0.05 kPa, and the maximum range of weighing instrument is 500 g, Minimum resolution is 0.0001 g.
5. microfluid flow on-line control device according to claim 1, it is characterised in that the adjusting model of microfluid flow It encloses for the mL/min of 0.002 mL/min~120.
6. a kind of utilizing microfluid flow online test method described in claim 1-5 any claims, it is characterised in that institute It is as follows to state method and step:
One, gas supply air source enters the first liquid container for filling liquid by pressure reducing valve and proportional pressure valve, holds in the first liquid Certain gas driving pressure is formed in device;
Two, as gas driving pressure increases, the liquid of the first liquid container flows into microfluid under air pressure driving by valve Channel is measured the entrance and exit pressure of microfluidic channel by first pressure sensor and second pressure sensor, obtained respectively The pressure difference at microfluidic channel both ends, and calculate the theoretical delivery of microfluid;
Three, microprocessor acquires the pressure signal of first pressure sensor and second pressure sensor in real time, while giving ratio pressure Power valve exports control instruction signal, changes the gas control pressure of proportional pressure valve, on-line control microfluid flow, and by weighing Instrument measures second liquid liquid in containers quality and changes with time rule, obtains the actual flow of microfluid, realizes microfluid The on-line checking of flow.
7. microfluid flow online test method according to claim 6, it is characterised in that the length of PTFE pipes with it is interior The ratio between diameter is more than 20:1, and meet inside pipe the reynolds number Re of microfluidic flow<1.0, microfluid is in Laminar Flow State.
8. microfluid flow online test method according to claim 6, it is characterised in that the variation of PTFE round tube inside diameters The variation range of ranging from 0.1~1.0 mm, PTFE pipe length is 10.0~40.0 mm, microfluidic channel both ends pressure differenceΔP Variation range be 0.2~35.0 kPa, the on-line checking precision of microfluid flow reaches 0.1%.
9. microfluid flow online test method according to claim 6, it is characterised in that the online tune of microfluid flow The time is saved less than 0.5 s, the on-line control precision of microfluid flow reaches 0.1%.
CN201810559797.7A 2018-06-02 2018-06-02 Microfluid flow online adjusting device and detection method Active CN108679448B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810559797.7A CN108679448B (en) 2018-06-02 2018-06-02 Microfluid flow online adjusting device and detection method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810559797.7A CN108679448B (en) 2018-06-02 2018-06-02 Microfluid flow online adjusting device and detection method

Publications (2)

Publication Number Publication Date
CN108679448A true CN108679448A (en) 2018-10-19
CN108679448B CN108679448B (en) 2020-05-05

Family

ID=63809648

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201810559797.7A Active CN108679448B (en) 2018-06-02 2018-06-02 Microfluid flow online adjusting device and detection method

Country Status (1)

Country Link
CN (1) CN108679448B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110508339A (en) * 2019-08-30 2019-11-29 上海理工大学 A kind of microfluid sampling device of air pressure driving
CN112934282A (en) * 2021-03-26 2021-06-11 西北大学 Self-feedback high-flux microfluidic system and method

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4188968A (en) * 1977-10-28 1980-02-19 Johnson Controls, Inc. Flow system with pressure level responsive air admission control
CN101750194A (en) * 2010-01-18 2010-06-23 北京艾迪西暖通科技有限公司 Test platform for comprehensive performance of pressure-reducing valve
CN102840995A (en) * 2012-09-10 2012-12-26 临海市伟达汽车部件有限公司 Performance test analysis system for EGR (Exhaust Gas Recirculation) cooler
CN103091095A (en) * 2013-01-11 2013-05-08 浙江大学台州研究院 Pneumatic proportion pressure valve testing device
CN103775700A (en) * 2014-03-03 2014-05-07 北京航空航天大学 Pressure regulating valve
CN104696706A (en) * 2015-01-20 2015-06-10 哈尔滨工业大学 Micro fluid flow adjusting device based on air pressure drive
CN105278587A (en) * 2015-10-26 2016-01-27 哈尔滨工业大学 Multi-channel microfluidic pressure source
CN207297311U (en) * 2017-09-07 2018-05-01 北京长城华冠汽车科技股份有限公司 Test device and testboard bay

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4188968A (en) * 1977-10-28 1980-02-19 Johnson Controls, Inc. Flow system with pressure level responsive air admission control
CN101750194A (en) * 2010-01-18 2010-06-23 北京艾迪西暖通科技有限公司 Test platform for comprehensive performance of pressure-reducing valve
CN102840995A (en) * 2012-09-10 2012-12-26 临海市伟达汽车部件有限公司 Performance test analysis system for EGR (Exhaust Gas Recirculation) cooler
CN103091095A (en) * 2013-01-11 2013-05-08 浙江大学台州研究院 Pneumatic proportion pressure valve testing device
CN103775700A (en) * 2014-03-03 2014-05-07 北京航空航天大学 Pressure regulating valve
CN104696706A (en) * 2015-01-20 2015-06-10 哈尔滨工业大学 Micro fluid flow adjusting device based on air pressure drive
CN105278587A (en) * 2015-10-26 2016-01-27 哈尔滨工业大学 Multi-channel microfluidic pressure source
CN207297311U (en) * 2017-09-07 2018-05-01 北京长城华冠汽车科技股份有限公司 Test device and testboard bay

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110508339A (en) * 2019-08-30 2019-11-29 上海理工大学 A kind of microfluid sampling device of air pressure driving
CN112934282A (en) * 2021-03-26 2021-06-11 西北大学 Self-feedback high-flux microfluidic system and method

Also Published As

Publication number Publication date
CN108679448B (en) 2020-05-05

Similar Documents

Publication Publication Date Title
CA2498034C (en) Flow control system
US9393504B2 (en) Closed loop flow control of a HPLC constant flow pump to enable low-flow operation
US7927477B2 (en) Precision flow control system
CN104696706B (en) The microfluid stream quantity regulating device driven based on air pressure
CN108679448A (en) Microfluid flow on-line control device and detection method
EP1777515B1 (en) Flow meter with a metering device and a control unit
US20050109698A1 (en) Flow sensing apparatus
JP2008089575A5 (en)
US10919003B2 (en) Method and apparatus for dynamic gas mixture production
Bissig et al. Micro-flow facility for traceability in steady and pulsating flow
Zeng et al. Characterization of syringe-pump-driven versus pressure-driven microfluidic flows
US10830681B2 (en) Method and measuring apparatus for determining compressibility of a flowing fluid
CN109932283A (en) Non-newtonian fluid apparent viscosity measuring device and measuring method under high-rate of shear
US5567885A (en) Measuring fluid flow rate
CN103699160A (en) High-precision gas quantitative and constant-pressure supply device
CN104457920A (en) Device and method for dynamically calibrating liquid flow
JP2004226077A (en) Gas dilution system
RU167027U1 (en) DEVICE FOR DIVIDING OIL WELL PRODUCTS PRODUCT FLOW
US20220025879A1 (en) Miniaturized multiple channel pressure control system
Zeng et al. Closed-loop pressure feedback control of a pressure-driven microdroplet generator
US8707780B2 (en) Fluid mixture metering device including an arcuate path on the periphery of the impeller wheel
KR101958289B1 (en) Valve assembled flowmeter
CN110339878A (en) The device and method of power-law fluid volume flow in a kind of control microchannel
JPH07128211A (en) Capillary viscometer, viscosity measuring method by means of capillary viscometer, and quality control method of fluid
HU9803013A2 (en) Equipment for providing constant volume flow of a liquid

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant