CN108587907A - It is a kind of that there is safeguard structure and the cell culture vessel based on micro-fluidic chip - Google Patents

Abstract

The invention discloses a cell culture vessel with a protective structure and based on a microfluidic chip, comprising a vessel cover, a protective belt, a sucker foot, an anti-fragmentation layer, a rubber anti-slip ring, a first soft pad, a microfluidic chip and a culture plate , the underside of the dish cover is equipped with a dish body, and the inside of the dish cover is respectively provided with a heat insulation layer and a thermal insulation layer from top to bottom, the lower end of the protective belt is connected with a protective pad, and the protective pad is embedded in the dish body The lower end of the left side, the notch is embedded in the back of the dish body, the right side of the microfluidic chip is movably installed with a turret, and the bottom end of the turret is provided with a first hinge shaft, the first hinge The shaft is embedded in the back of the dish body, and the cultivation plate is embedded in the inner bottom of the dish body. The invention realizes the protection structure of the cell culture vessel based on the microfluidic chip, and is designed with a non-slip and shatterproof structure, and also enables the microfluidic chip to be used in a sealed culture vessel, greatly improving the success rate of cell culture .

Description

A cell culture vessel with a protective structure and based on a microfluidic chip

technical field

The invention relates to the field of cell culture vessels, in particular to a cell culture vessel with a protective structure and based on a microfluidic chip.

Background technique

A petri dish is a laboratory vessel used for the cultivation of microorganisms or cells, consisting of a flat disc-shaped bottom and a cover, generally made of glass or plastic, it was originally developed under the German biologist Robert Koch Working bacteriologist Julius Richard Petri was designed in 1887, so it is also called "Petri dish";

Microfluidic chip technology integrates the basic operation units such as sample preparation, reaction, separation, and detection in the process of biological, chemical, and medical analysis into a micron-scale chip, and automatically completes the entire analysis process. It has developed into a new research field interdisciplinary in biology, chemistry, medicine, fluid, electronics, materials, machinery and other disciplines;

The current cell culture vessels based on microfluidic chips are not equipped with protective structures and are easily broken. At the same time, because there is no anti-skid structure, they are also easy to slip during cleaning. At the same time, the current cell culture vessels based on microfluidic chips are affected by the environment. Obviously, cell culture is often damaged. In addition, when using a microfluidic chip, the culture vessel needs to be opened, so that the inside of the culture vessel is easily polluted, resulting in the inability of the cells to be cultured.

Contents of the invention

The object of the present invention is to provide a cell culture vessel with a protective structure and based on a microfluidic chip, to solve the above-mentioned background technology. A structure that mitigates the influence of the external environment on the internal environment of the culture vessel. In addition, there is no problem in designing a microfluidic chip that can be used in a closed situation.

In order to achieve the above object, the present invention provides the following technical solutions: a cell culture vessel with a protective structure and based on a microfluidic chip, including a vessel cover, a protective belt, a suction cup foot, an anti-fragmentation layer, a rubber anti-slip ring, a first cushion , a microfluidic chip and a culture plate, a dish body is installed on the underside of the dish cover, and the inside of the dish cover is respectively provided with a heat insulation layer and a heat preservation layer from top to bottom, and a protective pad is connected to the lower end of the protective belt , and the protective pad is inlaid on the left lower end of the dish body, the upper end of the suction cup foot is fixedly connected to the dish body, the anti-shatter layer is embedded in the inside of the dish body, and the rubber anti-slip ring is wrapped around the lower end of the dish body, The bottom end of the first soft pad is installed on the back of the dish, and the left side of the first soft pad is provided with a notch, the notch is embedded in the back of the dish, and the right side of the microfluidic chip A turret is movably installed, and the bottom end of the turret is provided with a first hinged shaft, the first hinged shaft is embedded in the back of the dish body, and the cultivation tray is embedded in the inner bottom end of the dish body.

Preferably, the protective pads are elastic rubber pads, and there are eight sets of protective pads in total, and the protective pads are respectively embedded in the eight corners of the dish body.

Preferably, the outer surface of the rubber anti-slip ring is provided with concave-convex lines, and the rubber anti-slip ring is wrapped on the outer surface of the lower end of the dish.

Preferably, the first soft pad is provided with a raised soft block, and the outer size of the raised soft block is the same as the inner size of the notch, and the first soft pad and the notch have an embedded connection structure.

Preferably, the turret includes a telescopic bracket, a fixing bolt, a second hinge shaft, a connecting bracket, a second cushion and a chuck, the middle part of the telescopic bracket is provided with a fixing bolt, and the telescopic bracket is connected to the The brackets are connected, and the left side of the telescopic chuck is installed on the connecting bracket.

Preferably, the first hinged shaft is embedded in the back of the dish, and the upper end of the first hinged shaft is connected to a turret, and the dish is connected to the turret through the first hinged shaft in a rotatable connection structure.

Compared with the prior art, the beneficial effect of the present invention is: the cell culture vessel with a protective structure and based on the microfluidic chip is designed in combination with the structure of the traditional microfluidic chip-based cell culture vessel, considering its A non-slip and shatter-resistant structure is required, and a structure that can slow down the impact of the external environment on the internal environment of the culture vessel, and at the same time, the corresponding structural design can be carried out according to the requirements of the microfluidic chip in a sealed condition;

(1) There are heat insulation layer, heat preservation layer and shatterproof layer. The three-layer structure is attached and pressed together by thermoplastic lamination. The heat insulation layer is a glass fiber layer, and the heat preservation layer is a silicon aluminum fiber layer, which is shatterproof. The layer is a UV glue adhesive layer, and the heat insulation layer can effectively avoid the influence of the external environment on the internal environment of the culture vessel, and slow down the transfer of external temperature to the inside of the culture vessel. At the same time, the insulation layer can keep the temperature inside the culture vessel at a certain level. Within a certain range, the mutual cooperation of the insulation layer and the insulation layer makes the culture vessel have a good internal environment during the time period of culturing cells, and also avoids the influence of the external environment on the culture vessel, which greatly improves the success rate of culturing cells , In addition, the design of the anti-shatter layer makes the culture vessel not broken even if it is subjected to severe collision during use, which improves the safety of the culture vessel.

(2) Protective pads, protective belts and rubber anti-slip rings are provided. When the operator uses the culture vessel, due to the frequent use of culture fluid, liquid remains on the outside of the culture vessel or on the hands of the operator. When moving the culture vessel, It is easy to cause the culture vessel to slip, and the rubber anti-slip ring is wrapped around the lower end of the vessel body, and the rubber anti-slip ring is provided with concave and convex lines, which increases the friction between the operator's hand and the culture vessel, making it difficult to move the culture vessel. In addition, if the operator accidentally drops the culture vessel, the protective pads and protective belts embedded in the eight corners of the vessel can slow down the impact of the vessel when it falls, thereby preventing the vessel from being damaged;

(3) A turret is installed so that the microfluidic chip can be used while keeping the dish body sealed. The operator puts the culture solution into the culture plate in the dish body, then closes the dish cover, and puts the first plate on the back of the dish body The soft pad is pulled up from the notch, and then the microfluidic chip is fixed in the chuck on the turret, and the fixing bolt is rotated to stretch the telescopic bracket to a suitable position, and then pass through the first hinge axis and the second hinge axis Move the turret, push the microfluidic chip from the slot into the inside of the dish, and then seal the slot with the second cushion installed on the connecting bracket. This design can make the dish body sealed. The microfluidic chip prevents the inside of the dish from being polluted by external factors when culturing cells, and improves the success rate of cell culture.

Description of drawings

Fig. 1 is the front view sectional schematic diagram of structure of the present invention;

Fig. 2 is a schematic front view of the structure of the present invention;

Fig. 3 is a schematic top view of the structure of the present invention;

Fig. 4 is a schematic side view of the turret structure of the present invention.

In the figure: 1. Dish cover; 2. Dish body; 3. Heat insulation layer; 4. Insulation layer; 5. Protective belt; 6. Protective pad; 7. Suction cup feet; ; 10, the first cushion; 11, the notch; 12, the microfluidic chip; 13, the turret; 131, the telescopic bracket; 132, the fixing bolt; 133, the second hinge shaft; 134, the connecting bracket; 135, the first Two soft pads; 136, the chuck; 14, the first hinged shaft; 15, the cultivation tray.

Detailed ways

The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

Please refer to Fig. 1-4, a kind of embodiment provided by the present invention:

A cell culture vessel with a protective structure and based on a microfluidic chip, including a vessel cover 1, a protective belt 5, a suction cup foot 7, an anti-fragmentation layer 8, a rubber anti-slip ring 9, a first soft pad 10, and a microfluidic chip 12 And culture dish 15, dish body 2 is installed on the underside of dish cover 1, and the inside of dish cover 1 is respectively provided with insulation layer 3 and insulation layer 4 from top to bottom, heat insulation layer 3, insulation layer 4 and shatterproof Layer 8, the three-layer structure is attached and pressed together by thermoplastic lamination, the heat insulation layer 3 is a glass fiber layer, the heat insulation layer 4 is a silicon aluminum fiber layer, and the anti-shatter layer 8 is a UV glue adhesive layer, heat insulation Layer 3 can effectively avoid the influence of the external environment on the internal environment of the culture vessel, and slow down the transfer of external temperature to the inside of the culture vessel. At the same time, the insulation layer 4 can keep the temperature inside the culture vessel within a certain range. The heat insulation layer 3 and The mutual cooperation of the insulation layer 4 makes the culture vessel have a good internal environment during the time period of culturing cells, and also avoids the influence of the external environment on the culture vessel, so that the success rate of culturing cells is greatly improved. In addition, the shatterproof layer 8 design, so that the culture vessel will not be broken even if it is subjected to severe collision during use, which improves the safety of the culture vessel;

The lower end of the protective belt 5 is connected with a protective pad 6, and the protective pad 6 is inlaid on the left lower end of the dish body 2, the upper end of the suction cup foot 7 is fixedly connected with the dish body 2, and the anti-shatter layer 8 is embedded in the inside of the dish body 2, Rubber anti-skid ring 9 is wrapped in the lower end of dish body 2, and protective pad 6 is an elastic rubber pad, and protective pad 6 is provided with eight groups altogether, and protective pad 6 is embedded in eight corners of dish body 2 respectively, and rubber anti-skid ring 9 The outer surface of the outer surface is provided with concave and convex lines, and the rubber anti-slip ring 9 is wrapped on the outer surface of the lower end of the dish body 2. When the operator uses the culture vessel, due to the frequent use of culture solution, there will be residues on the outside of the culture vessel or on the hands of the operator. If there is liquid, when the culture vessel is moved, it is easy to cause the culture vessel to slip, and the rubber anti-slip ring 9 is wrapped around the lower end of the dish body 2, and the rubber anti-slip ring 9 is provided with concave-convex lines, which increases the operator's hands. The frictional force of the vessel makes it difficult to slip when moving the culture vessel. In addition, if the operator accidentally drops the culture vessel, the protective pads 6 and protective belts 5 embedded in the eight corners of the vessel body 2 can slow down the movement of the vessel body 2. The impact force received when falling, so as to avoid the dish body 2 from being damaged;

The bottom end of the first soft pad 10 is installed on the back side of the dish body 2, and the left side of the first soft pad 10 is provided with a notch 11, and the notch 11 is embedded in the back side of the dish body 2, and the first soft pad 10 is provided with There is a raised soft block, and the outer size of the raised soft block is the same as the inner size of the notch 11, the first soft pad 10 and the notch 11 are embedded connection structures, and the first soft pad 10 is embedded in the notch 11 , to ensure the tightness of the dish body 2, a turret 13 is movably installed on the right side of the microfluidic chip 12, and the bottom end of the turret 13 is provided with a first hinge shaft 14, and the first hinge shaft 14 is embedded in the dish body 2, the cultivation plate 15 is embedded in the inner bottom of the dish body 2, and the turret 13 includes a telescopic bracket 131, a fixing bolt 132, a second hinge shaft 133, a connecting bracket 134, a second cushion 135 and a chuck 136, The middle part of the telescopic bracket 131 is provided with a fixing bolt 132, and the telescopic bracket 131 is connected with the connecting bracket 134 through the second hinge shaft 133, and the left side of the telescopic chuck 136 is installed on the connecting bracket 134, and the operator puts the culture solution into the In the culture tray 15 in the dish body 2, close the dish cover 1 subsequently, pull up the first cushion 10 on the back side of the dish body 2 from the notch 11, and then fix the microfluidic chip 12 on the clip on the turret 13 In the head 136, the fixed bolt 132 is rotated to stretch the telescopic support 131 to a suitable position, and then the turret 13 is moved through the first hinge shaft 14 and the second hinge shaft 133, and the microfluidic chip 12 is lifted out of the slot 11. Push it into the inside of the dish body 2, and then, the second cushion 135 installed on the connecting bracket 134 seals the notch 11. This design can make the microfluidic chip 12 used when the dish body 2 is sealed, avoiding The inside of the dish body 2 is polluted by external factors when culturing cells, which improves the success rate of cell culturing.

It will be apparent to those skilled in the art that the invention is not limited to the details of the above-described exemplary embodiments, but that the invention can be embodied in other specific forms without departing from the spirit or essential characteristics of the invention. Accordingly, the embodiments should be regarded in all points of view as exemplary and not restrictive, the scope of the invention being defined by the appended claims rather than the foregoing description, and it is therefore intended that the scope of the invention be defined by the appended claims rather than by the foregoing description. All changes within the meaning and range of equivalents of the elements are embraced in the present invention. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims (6)

1. A cell culture vessel with a protective structure and based on a microfluidic chip, including a vessel cover (1), a protective belt (5), a suction cup foot (7), an anti-shatter layer (8), and a rubber anti-slip ring (9) , a first cushion (10), a microfluidic chip (12) and a culture plate (15), characterized in that: a dish body (2) is installed on the underside of the dish cover (1), and the dish cover (1 ) are respectively provided with a heat insulation layer (3) and a heat insulation layer (4) from top to bottom, and the lower end of the protective belt (5) is connected with a protective pad (6), and the protective pad (6) is embedded in the dish body (2), the upper end of the suction cup foot (7) is fixedly connected with the dish body (2), the anti-shatter layer (8) is embedded in the inside of the dish body (2), and the rubber anti-skid ring (9) wrapped in the lower end of the dish body (2), the bottom end of the first cushion (10) is installed on the back of the dish body (2), and the left side of the first soft cushion (10) is provided with a notch (11), the notch (11) is embedded in the back side of the dish body (2), and the right side of the microfluidic chip (12) is movably equipped with a turret (13), and the turret (13) The bottom end is provided with a first hinge shaft (14), the first hinge shaft (14) is embedded in the back of the dish body (2), and the cultivation plate (15) is embedded in the inner bottom of the dish body (2). end. 2. A kind of cell culture vessel with protective structure and based on microfluidic chip according to claim 1, characterized in that: the protective pad (6) is an elastic rubber pad, and the protective pad (6) is provided with Eight groups, protective pads (6) are inlaid at eight corners of the dish body (2) respectively. 3. A kind of cell culture vessel with protective structure and based on microfluidic chip according to claim 1, characterized in that: the outer surface of the rubber anti-slip ring (9) is provided with concave and convex lines, and the rubber anti-slip ring (9) 9) wrapped on the outer surface of the lower end of the dish body (2). 4. A cell culture vessel with a protective structure and based on a microfluidic chip according to claim 1, characterized in that: the first cushion (10) is provided with a raised soft block, and the raised The outer dimensions of the soft block are the same as the inner dimensions of the notch (11), and the first soft pad (10) and the notch (11) are embedded connection structures. 5. A cell culture vessel with a protective structure and based on a microfluidic chip according to claim 1, characterized in that: the turret (13) includes a telescopic bracket (131), a fixing bolt (132), a second Two articulated shafts (133), connecting bracket (134), second cushion (135) and chuck (136), the middle part of telescopic bracket (131) is provided with fixing bolt (132), and telescopic bracket (131) passes through The second hinge shaft (133) is connected with the connecting bracket (134), and the left side of the telescopic chuck (136) is installed on the connecting bracket (134). 6. A cell culture vessel with a protective structure and based on a microfluidic chip according to claim 1, characterized in that: the first hinge shaft (14) is embedded in the back of the vessel body (2), and The upper end of the first hinge shaft (14) is connected with a turret (13), and the dish body (2) is a rotary connection structure with the turret (13) through the first hinge shaft (14).