CN108522543A - A kind of tuniclike psammosilene root root rot biological control method - Google Patents
A kind of tuniclike psammosilene root root rot biological control method Download PDFInfo
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- CN108522543A CN108522543A CN201810581224.4A CN201810581224A CN108522543A CN 108522543 A CN108522543 A CN 108522543A CN 201810581224 A CN201810581224 A CN 201810581224A CN 108522543 A CN108522543 A CN 108522543A
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- root
- tuniclike psammosilene
- control method
- irrigation
- biological control
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
Abstract
The present invention provides a kind of tuniclike psammosilene root root rot biological control method, control method includes the following steps, (1) preparation of reagents：It is mother liquor to take the aqua of 100 liters of lactic acid bacterias, adds 1000g bacillus pulvis and 1000 grams of common sucroses successively into mother liquor；It is slowly stirred uniformly, then adds 1000ml acetic acid aquas, adjust solution ph, stir evenly spare；(2) irrigation liquid is prepared：The reagent that step (1) makes is taken according to the irrigation norm of 30L/hm2, is then diluted with water, obtains irrigation liquid, it is spare；(3) it irrigates for the first time：Tuniclike psammosilene root root is irrigated at initial stage in rainy season, is irrigated in tuniclike psammosilene root root laying plastic；(4) it irrigates for second：Second tuniclike psammosilene root root is irrigated at September end, mulch is reverted into origin-location after irrigation；Biological control method provided by the invention can effectively prevent tuniclike psammosilene root root rot, and lasting effect is good, at low cost compared to chemical method.
Description
Technical field
The present invention relates to Chinese herbal medicine cultivation technology fields, and in particular to a kind of tuniclike psammosilene root root rot biological control method.
Background technology
The technology of the present invention discloses a kind of tuniclike psammosilene root (Classification system：Psammosilene tunicoides) root-rot diease occurrence
Object control method.Medicinal plant tuniclike psammosilene root ((Classification system of the present invention：Psammosilene tunicoides), it is stone
A kind of herbaceos perennial that bamboo section tuniclike psammosilene root belongs to, subterraneous root shape, rich in amino acid, organic acid, saponin, triterpene, toxic, custom
Claim " malicious stator " root hyoscine, there is the effect of expelling wind and activating blood flow, removing blood stasis and analgesics.For traumatic injury, rheumatalgia pain, stomachache；External application
Control traumatic bleeding etc..As the market demand increases, wild resource is excessively excavated, wild resource is exhausted, switchs to artificial growth, collects
In intensive plantation, tuniclike psammosilene root declines Resistance To Root Rot Disease, the sheet of morbidity death in local plot occurs, causes seriously to damage
It loses, it is average also 20.4% generally in 10%-38%, it is one of an important factor for causing tuniclike psammosilene root heavy losses.It was preventing
Cheng Zhong has largely got preferable effect using Agro-chemicals control.But with administration time, increase to number, controls bacterium effect
It continuously decreases, mainly since the drug resistance of germ is stepped up, disease is made to be on the rise.There is an urgent need for research and develop a kind of tuniclike psammosilene root root-rot
Sick biological prevention establishes stable beneficial microbe dominant microflora in the soil using bio-active bacteria (fungi), generates resistance object
Matter is fought with germ, reaches antibacterial harmful levels of pathogens pythium (Pythium) and Fusarium (Fusarium Link ex
Fr.), Syst.Mycol, 1, XLI (introduct.), etc. to which purpose is applied to reaching lasting prevention tuniclike psammosilene root root rot
It produces, not only sustainable raising control effect and reduction cost accounting, but also efficiency can be improved.
Invention content
In view of the deficiencies of the prior art, the present invention provides a kind of tuniclike psammosilene root root rot biological control method, the preventions
Method includes the following steps,
(1) preparation of reagents：It is mother liquor to take the aqua of 100 liters of lactic acid bacterias, adds 1000g bacillus successively into mother liquor
Pulvis and 1000 grams of common sucroses；It is slowly stirred uniformly, then adds 1000ml acetic acid aquas, adjust solution ph, stir evenly
It is spare；
(2) irrigation liquid is prepared：The reagent that step (1) makes is taken according to the irrigation norm of 30L/hm2, then uses water dilute
It releases, obtains irrigation liquid, it is spare；
(3) it irrigates for the first time：Tuniclike psammosilene root root is irrigated at initial stage in rainy season, irrigates and is laid with ground in tuniclike psammosilene root root
Film；
(4) it irrigates for second：Second tuniclike psammosilene root root is irrigated at September end, mulch is reverted into original after irrigation
Come position.
Preferably, solution ph is 6.0-6.5 in above-mentioned steps (1).
Preferably, above-mentioned steps (2) reagent is 1 according to volume ratio with water:150 dilutions.
Preferably, bacillus pulvis bacillus quantity-quality density is 1,000,000,000/g's in above-mentioned steps (1).
Preferably, Bacillus acidi lactici quantity bulk density is 200,000,000/ml in Bacillus acidi lactici aqua in above-mentioned steps (1).
Preferably, first time irrigation time is June in above-mentioned steps (3).
Beneficial effects of the present invention are：
(1) present invention establishes dominant microflora using bacteria agent self-reproduction；In June and September respectively to tuniclike psammosilene root
Pouring root is carried out, allows lactic acid bacteria and bacillus using glucose as energy substance mass propagation, generates lactic acid bacteria and bacillus
Dominant microflora largely generates resisting substance lipopeptid and lactic acid to inhibit the numerous of pathogenic bacteria using lactic acid bacteria and bacillus flora
It grows, to reach with controlling pathogenic germ purpose.The composite bacteria agent that the compounding of bacillus and lactic acid bacteria generates, expands
Inhibit bacterium spectrum, expands disease-control face；The biological method pollution reduced to environment more significant than chemical prevention and control method is used simultaneously；
(2) the microbial inoculum preparation method is simple, easy to produce.City may be used in bacillus, lactic acid bacteria aqua and glucose
Product is sold, it is glucose sugar that lactobacter growth, which expands numerous required energy, activates the bacillus of suspend mode, is made into active bio microbial inoculum,
Suitable acidity is adjusted with acetic acid, keeps activity at normal temperatures, raw material sources are extensive, of low cost.
(3) a large amount of organic matters are rich in the soil of tuniclike psammosilene root growth, which can be used as bacillus and lactic acid bacteria
Energy substance, so tuniclike psammosilene root planting soil can promote lactic acid bacteria and bacillus to breed and establish flora.；Lactic acid simultaneously
The macromolecular substances for being difficult to be washed absorption by tuniclike psammosilene root root can also be resolved into the decomposition of organic matter by bacterium and bacillus to be easy to
Absorbed small molecule promotes the growth of tuniclike psammosilene root plant, so tuniclike psammosilene root plant and flora can mutually promote.
(4) this method is at low cost, easily promotes, has a good application prospect.
Specific implementation mode
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the implementation of the present invention
Example, technical scheme in the embodiment of the invention is clearly and completely described.Based on the embodiments of the present invention, this field
The every other embodiment that those of ordinary skill is obtained without creative efforts, belongs to protection of the present invention
Range.
Embodiment 1：
The present invention provides a kind of tuniclike psammosilene root root rot biological control method, the control method includes the following steps,
(1) preparation of reagents：It is female to take the aqua for the lactic acid bacteria that 100 liters of Bacillus acidi lactici quantity bulk densities are 200,000,000/ml
Liquid adds pulvis and 1000 grams of common sucroses that 1000g bacillus quantity-quality density is 1,000,000,000/g into mother liquor successively；It is slow
Slowly it stirs evenly, then adds 1000ml acetic acid aquas, it is 6.0 to adjust solution ph, is stirred evenly spare；
(2) irrigation liquid is prepared：According to 30L/hm2Irrigation norm take the reagent that step (1) makes, then pressed with water
It is 1 according to volume ratio:150 dilutions, obtain irrigation liquid, spare；
(3) it irrigates for the first time：Tuniclike psammosilene root root is irrigated at initial stage in rainy season, irrigates and is laid with ground in tuniclike psammosilene root root
Film；
(4) it irrigates for second：Second tuniclike psammosilene root root is irrigated at September end, mulch is reverted into original after irrigation
Come position.
Embodiment 2：
The present invention provides a kind of tuniclike psammosilene root root rot biological control method, the control method includes the following steps,
(1) preparation of reagents：It is female to take the aqua for the lactic acid bacteria that 100 liters of Bacillus acidi lactici quantity bulk densities are 200,000,000/ml
Liquid adds pulvis and 1000 grams of common sucroses that 1000g bacillus quantity-quality density is 1,000,000,000/g into mother liquor successively；It is slow
Slowly it stirs evenly, then adds 1000ml acetic acid aquas, it is 6.1 to adjust solution ph, is stirred evenly spare；
(2) irrigation liquid is prepared：According to 30L/hm2Irrigation norm take the reagent that step (1) makes, then pressed with water
It is 1 according to volume ratio:150 dilutions, obtain irrigation liquid, spare；
(3) it irrigates for the first time：Tuniclike psammosilene root root is irrigated at initial stage in rainy season, irrigates and is laid with ground in tuniclike psammosilene root root
Film；
(4) it irrigates for second：Second tuniclike psammosilene root root is irrigated at September end, mulch is reverted into original after irrigation
Come position.
Embodiment 3：
The present invention provides a kind of tuniclike psammosilene root root rot biological control method, the control method includes the following steps,
(1) preparation of reagents：It is female to take the aqua for the lactic acid bacteria that 100 liters of Bacillus acidi lactici quantity bulk densities are 200,000,000/ml
Liquid adds pulvis and 1000 grams of common sucroses that 1000g bacillus quantity-quality density is 1,000,000,000/g into mother liquor successively；It is slow
Slowly it stirs evenly, then adds 1000ml acetic acid aquas, it is 6.2 to adjust solution ph, is stirred evenly spare；
(2) irrigation liquid is prepared：According to 30L/hm2Irrigation norm take the reagent that step (1) makes, then pressed with water
It is 1 according to volume ratio:150 dilutions, obtain irrigation liquid, spare；
(3) it irrigates for the first time：Tuniclike psammosilene root root is irrigated at initial stage in rainy season, irrigates and is laid with ground in tuniclike psammosilene root root
Film；
(4) it irrigates for second：Second tuniclike psammosilene root root is irrigated at September end, mulch is reverted into original after irrigation
Come position.
Embodiment 4：
The present invention provides a kind of tuniclike psammosilene root root rot biological control method, the control method includes the following steps,
(1) preparation of reagents：It is female to take the aqua for the lactic acid bacteria that 100 liters of Bacillus acidi lactici quantity bulk densities are 200,000,000/ml
Liquid adds pulvis and 1000 grams of common sucroses that 1000g bacillus quantity-quality density is 1,000,000,000/g into mother liquor successively；It is slow
Slowly it stirs evenly, then adds 1000ml acetic acid aquas, it is 6.3 to adjust solution ph, is stirred evenly spare；
(2) irrigation liquid is prepared：According to 30L/hm2Irrigation norm take the reagent that step (1) makes, then pressed with water
It is 1 according to volume ratio:150 dilutions, obtain irrigation liquid, spare；
(3) it irrigates for the first time：Tuniclike psammosilene root root is irrigated at initial stage in rainy season, irrigates and is laid with ground in tuniclike psammosilene root root
Film；
(4) it irrigates for second：Second tuniclike psammosilene root root is irrigated at September end, mulch is reverted into original after irrigation
Come position.
Embodiment 5：
The present invention provides a kind of tuniclike psammosilene root root rot biological control method, the control method includes the following steps,
(1) preparation of reagents：It is female to take the aqua for the lactic acid bacteria that 100 liters of Bacillus acidi lactici quantity bulk densities are 200,000,000/ml
Liquid adds pulvis and 1000 grams of common sucroses that 1000g bacillus quantity-quality density is 1,000,000,000/g into mother liquor successively；It is slow
Slowly it stirs evenly, then adds 1000ml acetic acid aquas, it is 6.5 to adjust solution ph, is stirred evenly spare；
(2) irrigation liquid is prepared：According to 30L/hm2Irrigation norm take the reagent that step (1) makes, then pressed with water
It is 1 according to volume ratio:150 dilutions, obtain irrigation liquid, spare；
(3) it irrigates for the first time：Tuniclike psammosilene root root is irrigated at initial stage in rainy season, irrigates and is laid with ground in tuniclike psammosilene root root
Film；
(4) it irrigates for second：Second tuniclike psammosilene root root is irrigated at September end, mulch is reverted into original after irrigation
Come position.
1 bacteria agent field trial demonstration result of table
Field information experiment and demonstration is carried out in Kunming and Lijiang Prefecture using embodiment 1-5 provided by the invention, by a definite date two
Year, the results are shown in Table 1.As shown in Table 1, tuniclike psammosilene root root rot effect is reached using bacteria agent provided by the invention
87.6%, compare chemical prevention and control method, and bacteria agent continuous stability improves 11.5%, and cost reduction 65%.So
Biological antibiotic method provided by the invention is worthy to be popularized.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although with reference to the foregoing embodiments
Invention is explained in detail, it will be understood by those of ordinary skill in the art that：It still can be to aforementioned each implementation
Technical solution recorded in example is modified or equivalent replacement of some of the technical features；And these modification or
It replaces, the spirit and scope for various embodiments of the present invention technical solution that it does not separate the essence of the corresponding technical solution.
Claims (6)
1. a kind of tuniclike psammosilene root root rot biological control method, which is characterized in that the control method includes the following steps,
(1) preparation of reagents：It is mother liquor to take the aqua of 100 liters of lactic acid bacterias, adds 1000g bacillus pulvis successively into mother liquor
With 1000 grams of common sucroses；It is slowly stirred uniformly, then adds 1000ml acetic acid aquas, adjust solution ph, stir evenly spare；
(2) irrigation liquid is prepared：According to 30L/hm2Irrigation norm take the reagent that step (1) makes, be then diluted with water, obtain
It is spare to irrigation liquid；
(3) it irrigates for the first time：Tuniclike psammosilene root root is irrigated at initial stage in rainy season, is irrigated in tuniclike psammosilene root root laying plastic；
(4) it irrigates for second：Second tuniclike psammosilene root root is irrigated at September end, mulch is reverted into original position after irrigation
It sets.
2. tuniclike psammosilene root root rot biological control method according to claim 1, which is characterized in that molten in the step (1)
Liquid pH value is 6.0-6.5.
3. tuniclike psammosilene root root rot biological control method according to claim 1, which is characterized in that step (2) reagent
With water according to volume ratio be 1:150 dilutions.
4. tuniclike psammosilene root root rot biological control method according to claim 1, which is characterized in that bud in the step (1)
Spore bacillus pulvis bacillus quantity-quality density is 1,000,000,000/g's.
5. tuniclike psammosilene root root rot biological control method according to claim 1, which is characterized in that newborn in the step (1)
Bacillus acidi lactici quantity bulk density is 200,000,000/ml in acidfast bacilli aqua.
6. tuniclike psammosilene root root rot biological control method according to claim 1, which is characterized in that in the step (3)
Irrigation time is June.
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| CN201810581224.4A CN108522543A (en) | 2018-06-07 | 2018-06-07 | A kind of tuniclike psammosilene root root rot biological control method |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1766091A (en) * | 2005-09-23 | 2006-05-03 | 中国农业大学 | Bacillus subtilis and its uses |
| CN1993052A (en) * | 2004-07-29 | 2007-07-04 | 京都府 | Plant disease controlling agent and controlling method |
| CN101243801A (en) * | 2007-03-30 | 2008-08-20 | 上海泽元海洋生物技术有限公司 | Water dispersible powder of Paemobacillus polymyxa, preparation and application thereof |
| CN105475051A (en) * | 2015-12-23 | 2016-04-13 | 宁蒗县鑫玥农牧科技有限公司 | Method for preventing radix psammosilenes root rot disease |
| CN106386864A (en) * | 2016-08-30 | 2017-02-15 | 宁夏沃福百瑞枸杞产业股份有限公司 | Preparation for controlling root rot of lycium chinense and preparation and using method thereof |
-
2018
- 2018-06-07 CN CN201810581224.4A patent/CN108522543A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1993052A (en) * | 2004-07-29 | 2007-07-04 | 京都府 | Plant disease controlling agent and controlling method |
| CN1766091A (en) * | 2005-09-23 | 2006-05-03 | 中国农业大学 | Bacillus subtilis and its uses |
| CN101243801A (en) * | 2007-03-30 | 2008-08-20 | 上海泽元海洋生物技术有限公司 | Water dispersible powder of Paemobacillus polymyxa, preparation and application thereof |
| CN105475051A (en) * | 2015-12-23 | 2016-04-13 | 宁蒗县鑫玥农牧科技有限公司 | Method for preventing radix psammosilenes root rot disease |
| CN106386864A (en) * | 2016-08-30 | 2017-02-15 | 宁夏沃福百瑞枸杞产业股份有限公司 | Preparation for controlling root rot of lycium chinense and preparation and using method thereof |
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Application publication date: 20180914 |