CN108464392A - A kind of application of Dendrobium officinale polysaccharide in preparing probiotics - Google Patents
A kind of application of Dendrobium officinale polysaccharide in preparing probiotics Download PDFInfo
- Publication number
- CN108464392A CN108464392A CN201810213584.9A CN201810213584A CN108464392A CN 108464392 A CN108464392 A CN 108464392A CN 201810213584 A CN201810213584 A CN 201810213584A CN 108464392 A CN108464392 A CN 108464392A
- Authority
- CN
- China
- Prior art keywords
- application
- probiotics
- officinale polysaccharide
- dendrobium officinale
- time
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Polymers & Plastics (AREA)
- Biochemistry (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Animal Husbandry (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Medicinal Preparation (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a kind of application of Dendrobium officinale polysaccharide in preparing probiotics.Present invention demonstrates that Dendrobium officinale polysaccharide has intestinal flora important influence, beneficial bacterium can be promoted to grow, inhibit harmful bacteria, show that dendrobium candidum has the application value of medication in probiotics.
Description
Technical field
The invention belongs to probiotics preparing technical fields, and in particular to a kind of Dendrobium officinale polysaccharide is preparing Tiny ecosystem
Application in preparation.
Background technology
Current China has probiotics correlative study, predominantly the Tiny ecosystem system in farm animals edible additive
The probiotics correlative study of agent, people is less.Probiotics are individually composed by flora, or by flora and prebiotics
(such as oligosaccharide etc.) according to micro ecological active substance made of certain Process configuration, the main advantage of effect is to promote
It beneficial microbe colonizing in enteron aisle and is proliferated, reduces the quantity of sex pheromone in vivo, improve immunity of organisms, promote
Nutriment is digested and assimilated.Probiotics can play the high-quality effect and the two of flora and prebiotics itself inherently simultaneously
Between synergistic effect, to enhance digestion and the antibacterial ability of enteron aisle, and then enhance health.
Dendrobium candidum is orchid Dendrobium dendrobium candidum (Dendrobium officinaieKimuraetMigo.)
Drying stem.The effect of with " nourishing the stomach of promoting the production of body fluid, nourishing Yin and clearing heat, moistening lung kidney-nourishing, the strong waist of improving eyesight ".Dendrobium candidum it is main effectively at
It is divided into dendrobium polysaccharide and alkaloid.Dendrobium officinale polysaccharide extracts the method for mostly using water extract-alcohol precipitation, and stone is obtained by chemical analysis
Dry measure used in former times polysaccharide is mainly made of mannose, glucose, galactolipin, rhamnose and arabinose.In recent years, Dendrobium officinale polysaccharide is studied
It focuses mostly in antitumor and immunological regulation, but not yet influence research of the dendrobium polysaccharide to intestinal flora.
Currently, probiotics product preparation method is all to use conventional method, that is, probiotics is used only, or prebiotic
Prebiotics are added in bacterium, but are limited by the factors such as probiotics screening and probiotic active effect, constrain the hair of probiotics
Exhibition and application, and intestinal flora and the probiotics of Dendrobium officinale polysaccharide In Vitro Fermentation culture is used to grind accordingly not yet
Hair.
Invention content
It is an object of the invention to overcome prior art defect, a kind of Dendrobium officinale polysaccharide is provided and is preparing probiotics
In application.
Technical scheme is as follows：
A kind of application of Dendrobium officinale polysaccharide in preparing probiotics.
In a preferred embodiment of the invention, mannose, glucose, gala are contained in the Dendrobium officinale polysaccharide
Sugar, rhamnose and arabinose, preparation method are water extract-alcohol precipitation method.
It is further preferred that the water extraction and alcohol precipitation method includes：
(1) dendrobium candidum is cleaned, is dried, crushed；
(2) material obtained by step (1) is flowed back degreasing 1~2 time with 30%-60% petroleum ethers, every time 1~2h, every time
Petroleum ether dosage is 5~10 times of the weight of the dendrobium candidum；
(3) material obtained by step (2) is taken off into monosaccharide 1~2 time with 60~80% alcohol reflux, every time 2~3h, every time
Petroleum ether dosage is 5~10 times of the weight of the dendrobium candidum；
(4) water that 15~20 times of volumes are added into the material obtained by step (3) is decocted, and decocts 2~3 times, and every time 2
~3h will decoct obtained liquid after filtering every time, merge；
(5) 75~80% ethyl alcohol overnight precipitations are added into the material obtained by step (4) plus after the de- albumen of Sevage reagents,
Vacuum freeze drying is up to the Dendrobium officinale polysaccharide.
Still more preferably, which includes the following steps：
(1) culture medium is prepared, the formula of the culture medium is as follows：Contain the 3~6g of Dendrobium officinale polysaccharide in per 1000mL,
2~3g of peptone, yeast powder 2~3,2~3g of peptone, 0.2~0.3g of sodium chloride, phosphoric acid 0.02~0.04g of hydrogen dimethylamino, phosphoric acid
0.02~0.03g of potassium dihydrogen, 2~3g of sodium bicarbonate, 0.01~0.02g of magnesium sulfate, 0.02~0.03g of calcium chloride, ferroheme
0.002~0.003g, 0.002~0.003g of vitamin K, 0.5~0.6g of cholate, remaining is distilled water, pH 7.0；
(2) with above-mentioned culture medium to extracting from the intestinal flora in healthy fecal specimens anaerobic fermentation culture is carried out in 37 DEG C
24~48h obtains zymotic fluid；
(3) probiotics are made after above-mentioned zymotic fluid being lyophilized.
In a preferred embodiment of the invention, the dosage form of the probiotics be conventional tablet, enteric coated tablet,
Conventional capsule agent, enteric capsule, plain particles agent or enteric coated granule.
The beneficial effects of the invention are as follows：Present invention demonstrates that Dendrobium officinale polysaccharide has intestinal flora important influence, energy
Enough promote beneficial bacterium growth, inhibits harmful bacteria, show that dendrobium candidum has the application value of medication in probiotics.
Description of the drawings
Fig. 1 is the 16S rDNA sequencing result figures of the probiotics in zymotic fluid made from the embodiment of the present invention 1.
Specific implementation mode
Technical scheme of the present invention is further detailed and is described below by way of specific implementation mode combination attached drawing.
Embodiment 1
1, the preparation of Dendrobium officinale polysaccharide：
(1) dendrobium candidum is cleaned, is dried, crushed；
(2) by the 30%-60% petroleum ethers reflux degreasing 2 times, each 2h of the material obtained by step (1), each petroleum ether
Dosage is 5 times of the weight of the dendrobium candidum；
(3) material obtained by step (2) is taken off into monosaccharide 2 times, each 2h, each petroleum ether with 60~80% alcohol reflux
Dosage is 10 times of the weight of the dendrobium candidum；
(4) water that 20 times of volumes are added into the material obtained by step (3) is decocted, and is decocted 3 times, each 3h, will be every
It is secondary to decoct obtained liquid after filtering, merge；
(5) 80% ethyl alcohol overnight precipitation, vacuum is added into the material obtained by step (4) plus after the de- albumen of Sevage reagents
Be freeze-dried up to the Dendrobium officinale polysaccharide, the Dendrobium officinale polysaccharide contain mannose, glucose, galactolipin, rhamnose and
Arabinose.
2, probiotics are prepared
(1) culture medium is prepared, the formula of the culture medium is as follows：Contain the Dendrobium officinale polysaccharide 2g, egg in per 1000mL
White peptone 3g, sodium chloride 0.3g, phosphoric acid hydrogen dimethylamino 0.04g, potassium dihydrogen phosphate 0.03g, sodium bicarbonate 3g, magnesium sulfate 0.02g, chlorination
Calcium 0.03g, ferroheme 0.003g, vitamin K 0.003g, cholate 0.6g, remaining is distilled water, pH 7.0；
(2) with above-mentioned culture medium to extracting from (the attached middle mountain of source Xiamen University of the intestinal flora in healthy fecal specimens
The intestinal flora of hospital's GI Medicine separation) anaerobic fermentation culture 48h, acquisition zymotic fluid are carried out in 37 DEG C；Fermentation to gained
Liquid carries out 16S rDNA sequencings, and the results are shown in Figure 1, shows to cultivate beneficial bacterium in obtained flora and increases, and harmful bacteria is reduced,
And it is stable, reproducible using the probiotics flora ratio that this method makes；Gained zymotic fluid organic acid is examined
It surveys, as a result as shown in table 1 below, shows there is acetic acid, propionic acid, butyric acid and total organic acids content to have increase：
The organic acid testing result of zymotic fluid made from 1 embodiment of the present invention 1 of table
(3) will above-mentioned zymotic fluid freeze-drying after the probiotics are made, can be added protective agent, sustained release agent, corrigent and
Conventional tablet, enteric coated tablet, conventional capsule agent, enteric capsule, plain particles agent or enteric coated granule is made in excipient.
The zymotic fluid of gained in 2 above-described embodiment of embodiment is to intestinal bacilli illness Research of Animal Model for Study
1, intestinal bacilli illness Establishment of mouse model.
Select 60 6-8 week old C57BL/6 mouse, half male and half female, weight 18-22g, one group of daily gavage 2 times
0.25ml20mg/ml vancomycin normal saline solutions, for three days on end；Take stool in mice.
2, the recovery Regulating study of intestinal bacilli illness mouse model.
30 mouse are randomly selected in intestinal bacilli illness group as treatment group, for three days on end fermentation in gavage embodiment 1
Liquid, each 0.2ml, another 30 are control group, for three days on end gavage physiological saline, each 0.2ml；Take stool in mice.
By control experiment group and blank group, the mouse of the zymotic fluid gavage treatment is given, intestinal bacilli illness obtains
Restore, weight is increased, and shows that above-mentioned zymotic fluid has effects that adjust well and rebuilds intestinal flora, so as to effective
Treatment intestinal bacilli illness.
The foregoing is only a preferred embodiment of the present invention, therefore cannot limit the scope of implementation of the present invention according to this, i.e.,
According to equivalent changes and modifications made by the scope of the claims of the present invention and description, all should still belong in the range of the present invention covers.
Claims (5)
1. a kind of application of Dendrobium officinale polysaccharide in preparing probiotics.
2. application as described in claim 1, it is characterised in that：Contain mannose, glucose, half in the Dendrobium officinale polysaccharide
Lactose, rhamnose and arabinose, preparation method are water extract-alcohol precipitation method.
3. application as claimed in claim 2, it is characterised in that：The water extraction and alcohol precipitation method includes：
(1) dendrobium candidum is cleaned, is dried, crushed；
(2) material obtained by step (1) is flowed back degreasing 1~2 time with 30%-60% petroleum ethers, every time 1~2h, each oil
Ether dosage is 5~10 times of the weight of the dendrobium candidum；
(3) material obtained by step (2) is taken off into monosaccharide 1~2 time with 60~80% alcohol reflux, every time 2~3h, each oil
Ether dosage is 5~10 times of the weight of the dendrobium candidum；
(4) water that 15~20 times of volumes are added into the material obtained by step (3) is decocted, and decocts 2~3 times, every time 2~
3h will decoct obtained liquid after filtering every time, merge；
(5) 75~80% ethyl alcohol overnight precipitations, vacuum is added into the material obtained by step (4) plus after the de- albumen of Sevage reagents
It is freeze-dried up to the Dendrobium officinale polysaccharide.
4. application as claimed in claim 3, it is characterised in that：Include the following steps：
(1) culture medium is prepared, the formula of the culture medium is as follows：Contain the 3~6g of Dendrobium officinale polysaccharide, albumen in per 1000mL
2~3g of peptone, yeast powder 2~3,2~3g of peptone, 0.2~0.3g of sodium chloride, phosphoric acid 0.02~0.04g of hydrogen dimethylamino, biphosphate
0.02~0.03g of potassium, 2~3g of sodium bicarbonate, 0.01~0.02g of magnesium sulfate, 0.02~0.03g of calcium chloride, ferroheme 0.002~
0.003g, 0.002~0.003g of vitamin K, 0.5~0.6g of cholate, remaining is distilled water, pH 7.0；
(2) carried out with above-mentioned culture medium to extracting from the intestinal flora in healthy fecal specimens in 37 DEG C anaerobic fermentation culture 24~
48h obtains zymotic fluid；
(3) probiotics are made after above-mentioned zymotic fluid being lyophilized.
5. the application as described in any claim in Claims 1-4, it is characterised in that：The dosage form of the probiotics
For conventional tablet, enteric coated tablet, conventional capsule agent, enteric capsule, plain particles agent or enteric coated granule.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810213584.9A CN108464392A (en) | 2018-03-15 | 2018-03-15 | A kind of application of Dendrobium officinale polysaccharide in preparing probiotics |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810213584.9A CN108464392A (en) | 2018-03-15 | 2018-03-15 | A kind of application of Dendrobium officinale polysaccharide in preparing probiotics |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108464392A true CN108464392A (en) | 2018-08-31 |
Family
ID=63265387
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810213584.9A Pending CN108464392A (en) | 2018-03-15 | 2018-03-15 | A kind of application of Dendrobium officinale polysaccharide in preparing probiotics |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108464392A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109234332A (en) * | 2018-10-12 | 2019-01-18 | 华南理工大学 | A kind of method that solid state fermentation prepares high immunological activity dendrobium candidum endogenetic fungus polysaccharide |
| CN109432304A (en) * | 2018-12-03 | 2019-03-08 | 杭州胡庆余堂药业有限公司 | A kind of application of composition containing dendrobium candidum in medicine preparation |
| CN109527318A (en) * | 2018-11-29 | 2019-03-29 | 温岭市锦华铁皮石斛有限公司 | A kind of fresh breath solid beverage containing dendrobium candidum prebiotics and probiotics |
| CN116076726A (en) * | 2022-12-31 | 2023-05-09 | 浙江天方科技股份有限公司 | Dendrobium candidum polysaccharide solid instant direct-drinking powder and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104740337A (en) * | 2014-12-31 | 2015-07-01 | 浙江济公缘药业有限公司 | Dendrobium officinale extract as well as preparation method and application thereof |
| CN105622772A (en) * | 2016-01-12 | 2016-06-01 | 丽水学院 | Method for extracting dendrobium officinale polysaccharide based on solvent recycling |
| CN107286269A (en) * | 2017-08-11 | 2017-10-24 | 上海海洋大学 | Two kinds of preparation method and applications with immunoregulatory dendrobium candidum leaf polyose |
-
2018
- 2018-03-15 CN CN201810213584.9A patent/CN108464392A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104740337A (en) * | 2014-12-31 | 2015-07-01 | 浙江济公缘药业有限公司 | Dendrobium officinale extract as well as preparation method and application thereof |
| CN105622772A (en) * | 2016-01-12 | 2016-06-01 | 丽水学院 | Method for extracting dendrobium officinale polysaccharide based on solvent recycling |
| CN107286269A (en) * | 2017-08-11 | 2017-10-24 | 上海海洋大学 | Two kinds of preparation method and applications with immunoregulatory dendrobium candidum leaf polyose |
Non-Patent Citations (3)
| Title |
|---|
| 张冠亚: "铁皮石斛多糖在模拟消化、酵解体系中的代谢特点及其改善肠道功能的研究", 《中国优秀硕士学位论文全文数据库 工程科技I辑》 * |
| 梁建梅: "《医学检验基本操作技术》", 31 October 2013, 第二军医大学出版社 * |
| 谢寅峰: "稀土等对霍山石斛组培苗石斛多糖含量的影响", 《南京林业大学学报》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109234332A (en) * | 2018-10-12 | 2019-01-18 | 华南理工大学 | A kind of method that solid state fermentation prepares high immunological activity dendrobium candidum endogenetic fungus polysaccharide |
| CN109234332B (en) * | 2018-10-12 | 2022-04-22 | 华南理工大学 | Method for preparing high-immunocompetence dendrobium officinale endophytic fungi polysaccharide through solid state fermentation |
| CN109527318A (en) * | 2018-11-29 | 2019-03-29 | 温岭市锦华铁皮石斛有限公司 | A kind of fresh breath solid beverage containing dendrobium candidum prebiotics and probiotics |
| CN109432304A (en) * | 2018-12-03 | 2019-03-08 | 杭州胡庆余堂药业有限公司 | A kind of application of composition containing dendrobium candidum in medicine preparation |
| CN116076726A (en) * | 2022-12-31 | 2023-05-09 | 浙江天方科技股份有限公司 | Dendrobium candidum polysaccharide solid instant direct-drinking powder and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100489086C (en) | Lactic acid bacteria capable of stimulating mucosal immunity | |
| CN106794202B (en) | Improve the composition of human skin health and its prepares the purposes in product | |
| CN105707628B (en) | A kind of composition and preparation method thereof of probiotics fermention dendrobium candidum protocorm | |
| CN101575582B (en) | Lactobacillus separation strains with anti-inflammatory activity and application thereof | |
| CN108464392A (en) | A kind of application of Dendrobium officinale polysaccharide in preparing probiotics | |
| Sharma et al. | Efficacy and potential of lactic acid bacteria modulating human health | |
| KR20180103772A (en) | Composition for preventing or treating immune diseases comprising mixture of lactic acid bacteria | |
| CN109123295A (en) | A kind of probiotics solid beverage and preparation method thereof | |
| CN103908585B (en) | For the probiotics fermention compositions of prevention and therapy constipation | |
| CN102318794A (en) | Health-care food having calcium supplementing function | |
| CN105770066A (en) | Probiotic fermented traditional Chinese medicine compound preparation and preparation method and application thereof | |
| CN104870002B (en) | For preventing and/or the chitin for treating parasitic disease or derivatives thereof | |
| CN109628359A (en) | One plant of lactobacillus reuteri that can be relieved allergic asthma and its application | |
| CN1273838A (en) | Nutritive composition containing symbiotic fermentation culture medium of dead probiotics | |
| CN116814464B (en) | Fermented lactobacillus mucilaginosus JF5 and application thereof in preparation of lipid-reducing and digestion-aiding foods and medicines | |
| CN106420847A (en) | Composition for auxiliary protection of gastric mucosa and/or remission of gastrectasia and microecological preparation thereof | |
| CN103446552B (en) | For the fermentation composition of prevention and therapy digestive system disease | |
| CN104277999A (en) | Novel multiple-effect Clostridium butyricum and application thereof in aspects of enhancing oxidation resistance of animals and improving meat quality | |
| CN110521785A (en) | Probiotics fermention functional food and its preparation | |
| Yamin et al. | Effect of Ganoderma lucidum polysaccharides on the growth of Bifidobacterium spp. as assessed using real-time PCR. | |
| CN104432001A (en) | Edible composition and use thereof | |
| CN104739919A (en) | Fermented astragalus composite preparation as well as preparation method and application thereof | |
| CN109251877A (en) | It is a kind of with the bafillus natto Bn-SJLH001 prebiotic effect of the functions such as fat reducing and its application | |
| CN101301316B (en) | Compound formulation containing lactobacillus leavening and preparation and use thereof | |
| WO2020057451A1 (en) | Method for preparing compositions for treating hair loss |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180831 |