CN108450218A - A kind of seedling breeding method of oil tea - Google Patents
A kind of seedling breeding method of oil tea Download PDFInfo
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Abstract
Description
技术领域technical field
本发明涉及油茶的种苗繁育方法,属于农业种植技术领域。The invention relates to a seedling breeding method of camellia oleifera, belonging to the technical field of agricultural planting.
背景技术Background technique
油茶Camellia oleifera Abel,属山茶科油茶属小乔木树种。为我国特产的食用木本油料树种,茶油是国家颁布的《中国食物结构改革与发展规划纲要》要求大力发展的食用植物油。油茶还能通过油脂的深加工生产高级保健食用油和高级天然护肤化妆品等,茶油的副产品茶枯饼可提取茶皂素、制刨光粉和复合饲料,茶壳可提糠醛、鞣料和制活性炭等,通过综合利用还可以大大提高油茶经济效益。Camellia oleifera Abel is a small tree species of the genus Camellia in the family Theaceae. It is an edible woody oil tree species that is a specialty of our country, and camellia oil is an edible vegetable oil that is required to be vigorously developed in the "Outline of China's Food Structure Reform and Development Plan" promulgated by the state. Camellia oleifera can also produce high-grade health edible oil and high-grade natural skin care cosmetics through deep processing of oil. The by-product of camellia oil, tea dry cake, can extract tea saponin, make planed powder and compound feed, and tea shell can extract furfural, tanning materials and Activated carbon, etc., can also greatly improve the economic benefits of Camellia oleifera through comprehensive utilization.
目前,油茶种苗繁育常用芽苗砧嫁接技术,但传统油茶嫁接苗培育中存在着苗圃地严重的连作障碍和种苗成苗率低的问题,需要不断更换苗圃地,造成大量的土地闲置,浪费土地资源;且嫁接苗当年生长缓慢,当年生苗木生长质量差,出圃率低,育苗周期长,一般需两年时间以上。At present, bud seedling anvil grafting technology is commonly used in the breeding of camellia oleifera seedlings, but there are serious continuous cropping obstacles in the nursery and low seedling rate in the traditional cultivation of camellia oleifera seedlings, which requires constant replacement of the nursery, resulting in a large amount of idle land. Land resources are wasted; and the grafted seedlings grow slowly in the same year, the growth quality of the raw seedlings in the year is poor, the rate of emergence from the nursery is low, and the seedling cycle is long, generally needing more than two years.
发明内容Contents of the invention
本发明的目的在于解决现有技术中油茶种苗繁育技术的不足,提供一种油茶的种苗繁育方法,该方法不需要更换苗圃地,育苗成活率高,苗木生长质量好、出圃率高,并且缩短了育苗周期,降低了育苗成本。The purpose of the present invention is to solve the deficiency of Camellia oleifera seedling breeding technology in the prior art, provide a kind of seedling breeding method of Camellia oleifera, this method does not need to change the nursery field, the survival rate of seedling raising is high, the growth quality of seedlings is good, the nursery emergence rate is high, Moreover, the seedling raising period is shortened, and the seedling raising cost is reduced.
为了实现上述目标,本发明采用如下的技术方案:In order to achieve the above object, the present invention adopts the following technical solutions:
一种油茶的种苗繁育方法,包括以下步骤:A seedling propagation method of camellia oleifera, comprising the following steps:
(1)配制培养基质:按重量份称取如下组分:45-55份黄心土,30-40份泥炭土,3-9份珍珠岩,3-9份木屑,3-9份草木灰,经碾碎过筛,充分拌匀后,用多菌灵进行消毒处理,得到培养基质,备用;(1) Preparation of culture substrate: take the following components by weight: 45-55 parts of yellow heart soil, 30-40 parts of peat soil, 3-9 parts of perlite, 3-9 parts of sawdust, 3-9 parts of plant ash, after grinding Crushed and sieved, fully mixed, and sterilized with carbendazim to obtain a culture medium for later use;
(3)育苗种子处理:选用普通油茶的种子,在霜降节前后采摘,经晒后再阴干后脱出茶籽,挑选粒大饱满、无病虫害的种子做砧种,将种子用高锰酸钾溶液浸泡10~30min后进行贮藏,5月上旬,芽砧苗长好,即可进行嫁接;(3) Seed treatment for seedling cultivation: select the seeds of ordinary Camellia oleifera, pick them before and after the Frost's Descent Festival, and then dry them in the sun and then remove the tea seeds. Store after soaking in the solution for 10-30 minutes. In the first ten days of May, when the buds and anvils grow well, they can be grafted;
(3)苗圃地准备:选择交通便利、有水源,排水良好的地段做育苗圃地,开好边沟、中沟、步行道,作好苗床,苗床规格:床宽1~1.5m,长不超过16m,床高15~25cm,步道沟宽35~45cm,中沟、边沟深35~45cm;(3) Nursery site preparation: Choose an area with convenient transportation, water sources, and good drainage to make a nursery site, open side ditches, middle ditches, and walkways, and prepare seedbeds. Seedbed specifications: bed width 1-1.5m, length Over 16m, bed height 15-25cm, walkway ditch width 35-45cm, middle ditch and side ditch depth 35-45cm;
(4)接穗选择:选择母树树冠外围中、上部当年生的粗壮、腋芽饱满无病虫害的半木质化程度的新梢作为穗条,采后及时嫁接;(4) scion selection: select the young shoots of the semi-lignified degree that are thick, axillary buds full and free of disease and insect damage in the middle and upper part of the crown of the mother tree as the scion, and graft in time after harvesting;
(5)嫁接:5月上旬以后,采用芽苗砧嫁接技术,进行嫁接,嫁接完成后,将接好的油茶苗放在荫凉处以备栽植,并用湿布盖好,避免日光照射;(5) Grafting: After the first ten days of May, adopt the grafting technique of bud seedling anvil to carry out grafting. After the grafting is completed, put the camellia seedlings in the shade for planting, and cover them with a damp cloth to avoid sunlight;
(6)苗木栽植:在4月下旬至5月上旬,选用无纺布容器杯装填步骤(1)的培养基质,然后将填满培养基质的容器杯在苗圃地摆放整齐,容器杯间空隙用土填实,浇透水使容器杯中的培养基质沉实,在容器杯中用竹签插一个6~9cm深的小穴,再将嫁接好的油茶苗栽入杯中,向苗的方向回土,然后浇透水,小拱棚覆膜;(6) Seedling planting: From late April to early May, select the culture substrate of step (1) in the non-woven container cup filling step, and then arrange the container cups filled with the culture substrate neatly in the nursery. Fill it with soil, pour water thoroughly to make the culture medium in the container cup heavy, insert a small hole 6-9cm deep in the container cup with a bamboo stick, then plant the grafted Camellia oleifera seedlings into the cup, and return to the soil towards the seedlings , and then pour water thoroughly, and cover the small shed with film;
(7)架设荫棚:在栽植油茶嫁接苗的苗圃地架设荫棚,荫棚高:1.8~2.0m,棚株间距:3~5m,棚面用铁丝横拉作径,再覆盖遮阳网,并扎紧;(7) Set up shade sheds: set up shade sheds in the nursery where the grafted seedlings of camellia oleifera are planted, the height of the shade sheds: 1.8-2.0m, the distance between the sheds: 3-5m. and fasten;
(8)种植后管理:嫁接苗在膜内的时间为35~45d,以10%抽稍为准,揭膜后进行追肥,追肥时间为7~10月,每半月一次,以叶面喷洒方式实施,同时每月喷洒含有效成分2%多菌灵水溶液和0.05%的生长素水溶液;及时除草。(8) Post-planting management: the grafted seedlings stay in the film for 35 to 45 days, subject to 10% pumping, topdressing is carried out after the film is removed, and the topdressing time is from July to October, once every half a month, and implemented by spraying on the leaves , while spraying 2% carbendazim aqueous solution and 0.05% auxin aqueous solution containing active ingredients every month; weeding in time.
进一步,步骤(1)中,所述培养基质优选包括如下重量份的组分:50份黄心土,35份泥炭土,5份珍珠岩,5份木屑,5份草木灰。Further, in step (1), the culture substrate preferably includes the following components by weight: 50 parts of yellow core soil, 35 parts of peat soil, 5 parts of perlite, 5 parts of wood chips, and 5 parts of plant ash.
进一步,步骤(2)中,种子贮藏的方式是:选择地势较高、干净、平整、排水良好的场地,用砖砌宽0.9~1.2m,高30~40cm的催芽床,先在地面上洒少许水,再铺一层8~12cm的湿沙,然后铺一层种子,再盖上15~20cm厚的湿河沙,用清水喷透沙床,保持湿润。Further, in the step (2), the mode of seed storage is: select a higher terrain, clean, flat, well-drained site, use bricks to build a germination bed with a width of 0.9-1.2m and a height of 30-40cm, and sprinkle it on the ground first. A little water, then spread a layer of 8-12cm wet sand, then spread a layer of seeds, and then cover with 15-20cm thick wet river sand, spray the sand bed with clean water to keep it moist.
进一步,步骤(5)中,所述嫁接过程如下:Further, in step (5), the grafting process is as follows:
起砧:用手轻轻起芽砧苗,再用清水将芽砧苗上的沙子冲净,不能碰掉砧苗上的种子和碰断根部;Picking up the anvil: Gently lift up the anvil seedlings by hand, and then rinse the sand on the anvil seedlings with clean water, and do not touch off the seeds on the anvil seedlings or break the roots;
削穗:选用穗条上饱满的腋芽或顶芽,在腋芽两侧的下部0.5cm处下刀,削成两个斜面,削面长0.8~1.0cm,再在芽尖上部0.1~0.2cm处切断,便成一个接穗,接穗上的叶片削掉一半,将削好的接穗放在装有清水的盆内;Ear cutting: select the full axillary buds or terminal buds on the spikes, cut them into two slopes at the lower 0.5cm of both sides of the axillary buds, cut them into two slopes, the length of the cut surface is 0.8-1.0cm, and cut off at the upper part of the bud tip 0.1-0.2cm , it becomes a scion, half of the leaves on the scion are cut off, and the cut scion is placed in a basin filled with clear water;
削砧:在砧苗子叶柄上2~3cm处切断,对准中轴切下一刀,切口深1.0~1.2cm,砧苗根部保留5~6cm,将多余的部分切除;Cutting the anvil: Cut off at 2-3cm above the petiole of the anvil seedling, align the central axis and cut a knife, the depth of the incision is 1.0-1.2cm, keep the root of the anvil seedling 5-6cm, and cut off the excess part;
插穗和包扎:根据芽砧粗细,将接穗插入砧木的切口内,用铝片轻轻缠绕捏紧,即完成嫁接。Cuttings and wrapping: According to the thickness of the bud stock, insert the scion into the incision of the root stock, wrap it gently with an aluminum sheet and pinch it tightly, and the grafting is completed.
进一步,步骤(8)中,所述追肥所施的肥料配比为N2:K2O5:K2O=1:1:1,配成0.4~0.5%水溶液。Further, in the step (8), the ratio of the fertilizer applied for the topdressing is N 2 : K 2 O 5 : K 2 O = 1:1:1, and it is prepared as a 0.4-0.5% aqueous solution.
进一步,步骤(8)中,嫁接苗生长期间,环境温度控制在35℃以下,土壤始终保持湿润,空气湿度保持在80%,幼苗生长前期,严格控制光照条件,透光率在30~35%,随着幼苗生长和光照强度下降,到9月上旬以后,逐步增加透光量,10月中旬后,全光照。Further, in step (8), during the growth of grafted seedlings, the ambient temperature is controlled below 35°C, the soil is kept moist all the time, and the air humidity is kept at 80%. In the early stage of seedling growth, the light conditions are strictly controlled, and the light transmittance is 30-35%. , as the seedlings grow and the light intensity decreases, after the first ten days of September, gradually increase the amount of light transmission, and after mid-October, full light.
本发明的有益效果:提供了一种操作简单、方便易行的油茶种苗繁育方法,不需要更换苗圃地,并充分利用了当地资源来配制培养基质,大大降低了育苗成本;克服了育苗连作障碍,提高了苗圃利用率;短了育苗周期,传统油茶育苗一般需要2-3年,采用本技术,当年生种苗完全达到国家油茶苗造林用苗标准;提高了苗木质量和出圃率,嫁接成活率在95%以上,出圃率在90%以上,经济效益明显;管理方便,省工省力,本技术不需要建大棚等设施,管理简便,降低管理费用。Beneficial effects of the present invention: provide a simple, convenient and easy-to-operate Camellia oleifera seedling breeding method, do not need to replace the nursery field, and make full use of local resources to prepare the culture medium, greatly reducing the cost of seedling cultivation; overcome the continuous cropping of seedling cultivation Obstacles, improved nursery utilization rate; shortened seedling cultivation cycle, traditional Camellia oleifera seedlings generally need 2-3 years, using this technology, the current seedlings fully meet the national Camellia oleifera afforestation seedlings; improved seedling quality and nursery rate, grafting The survival rate is more than 95%, the rate of emergence is more than 90%, and the economic benefits are obvious; the management is convenient, labor and labor are saved, and the technology does not need to build greenhouses and other facilities, and the management is simple and the management cost is reduced.
具体实施方式Detailed ways
下面结合实施例对本发明作进一步详细的描述,但本发明的实施方式不限于此。The present invention will be further described in detail below in conjunction with examples, but the embodiments of the present invention are not limited thereto.
实施例1Example 1
2016年在河南省商城县长竹园乡育苗基地实施,嫁接苗品种为长林3号、4号、18号、40号和53号,按如下方法进行:In 2016, it was carried out in the seedling breeding base of Changzhuyuan Township, Shangcheng County, Henan Province. The varieties of grafted seedlings were Changlin No. 3, No. 4, No. 18, No. 40 and No. 53. The method was as follows:
一种油茶的种苗繁育方法,包括以下步骤:A seedling propagation method of camellia oleifera, comprising the following steps:
(1)配制培养基质:按重量份称取如下组分:50份黄心土,35份泥炭土,5份珍珠岩,5份木屑,5份草木灰,经碾碎过筛,充分拌匀后,用多菌灵进行消毒处理,得到培养基质,备用;(1) Preparation of culture substrate: take the following components by weight: 50 parts of yellow heart soil, 35 parts of peat soil, 5 parts of perlite, 5 parts of sawdust, 5 parts of plant ash, crushed and sieved, after fully mixing, use Carbendazim is sterilized to obtain the culture substrate for subsequent use;
(2育苗种子处理:选用普通油茶的种子,在霜降节前后采摘,经晒后再阴干后脱出茶籽,挑选粒大饱满、无病虫害的种子做砧种,以保证种子的发芽率和砧木的粗壮度;将种子用400mg/kg的高锰酸钾溶液浸泡20min,再进行贮藏,5月上旬,芽砧苗长好,即可进行嫁接;(2 Treatment of seedlings: select common Camellia oleifera seeds, pick them before and after the Frost's Descent Festival, and then dry them in the sun and then remove the tea seeds. Choose large and full seeds without pests and diseases as rootstocks to ensure the germination rate of the seeds and rootstocks. The robustness; soak the seeds in 400mg/kg potassium permanganate solution for 20 minutes, and then store them. In the first ten days of May, when the bud anvil seedlings grow well, they can be grafted;
种子贮藏的方式是:选择地势较高、干净、平整、排水良好的场地,用砖砌宽1m,高35cm的催芽床,先在地面上洒少许水,再铺一层10cm湿沙,然后放一层种子,再盖上15cm厚的湿河沙,以防种子干燥失水,3月上、中旬,用清水喷透沙床,保持湿润,湿度不够时及时喷水,到5月上旬即可进行嫁接。The method of seed storage is: choose a high-lying, clean, flat, and well-drained site, use bricks to build a germination bed with a width of 1m and a height of 35cm, first sprinkle a little water on the ground, then spread a layer of 10cm wet sand, and then put A layer of seeds, and then cover with 15cm thick wet river sand to prevent the seeds from drying out and losing water. In early and mid-March, spray the sand bed with clean water to keep it moist. When the humidity is not enough, spray water in time. It can be done in early May To graft.
(3)苗圃地准备:选择交通便利、有水源,排水良好的地段做育苗圃地,开好边沟、中沟、步行道,作好苗床。苗床规格:床宽1.2m,长不超过16m,床高20cm,步道沟宽40cm,中沟、边沟深40cm。(3) Nursery site preparation: Choose an area with convenient transportation, water sources, and good drainage to make a nursery site, open side ditches, middle ditches, and walkways, and prepare seedbeds. Seedbed specifications: bed width 1.2m, length no more than 16m, bed height 20cm, trail ditch width 40cm, middle ditch and side ditch depth 40cm.
(4)接穗选择:穗条应在采穗圃选择母树树冠外围中、上部当年生的粗壮、腋芽饱满无病虫害的半木质化程度的新梢,采后及时嫁接。(4) Scion selection: The scion should be selected in the scion picking nursery to select the new shoots of the middle and upper part of the crown of the mother tree that are thick, full of axillary buds, and semi-lignified without damage by diseases and insect pests, and grafted in time after harvest.
(5)嫁接:5月上旬以后,采用芽苗砧嫁接技术,进行嫁接,嫁接完成后,将接好的油茶苗放在荫凉处以备栽植,并用湿布盖好,避免日光照射;(5) Grafting: After the first ten days of May, adopt the grafting technique of bud seedling anvil to carry out grafting. After the grafting is completed, put the camellia seedlings in the shade for planting, and cover them with a damp cloth to avoid sunlight;
所述嫁接过程如下:The grafting process is as follows:
起砧:用手轻轻起芽砧苗,再用清水将芽砧苗上的沙子冲净,不能碰掉砧苗上的种子和碰断根部;Picking up the anvil: Gently lift up the anvil seedlings by hand, and then rinse the sand on the anvil seedlings with clean water, and do not touch off the seeds on the anvil seedlings or break the roots;
削穗:选用穗条上饱满的腋芽或顶芽,在腋芽两侧的下部0.5cm处下刀,削成两个斜面,削面长0.8~1.0cm,再在芽尖上部0.1~0.2cm处切断,便成一个接穗,接穗上的叶片削掉一半,将削好的接穗放在装有清水的盆内;Ear cutting: select the full axillary buds or terminal buds on the spikes, cut them into two slopes at the lower 0.5cm of both sides of the axillary buds, cut them into two slopes, the length of the cut surface is 0.8-1.0cm, and cut off at the upper part of the bud tip 0.1-0.2cm , it becomes a scion, half of the leaves on the scion are cut off, and the cut scion is placed in a basin filled with clear water;
削砧:在砧苗子叶柄上2~3cm处切断,对准中轴切下一刀,切口深1.0~1.2cm,砧苗根部保留5~6cm,将多余的部分切除;Cutting the anvil: Cut off at 2-3cm above the petiole of the anvil seedling, align the central axis and cut a knife, the depth of the incision is 1.0-1.2cm, keep the root of the anvil seedling 5-6cm, and cut off the excess part;
插穗和包扎:根据芽砧粗细,将接穗插入砧木的切口内,用铝片轻轻缠绕捏紧,即完成嫁接。Cuttings and wrapping: According to the thickness of the bud stock, insert the scion into the incision of the root stock, wrap it gently with an aluminum sheet and pinch it tightly, and the grafting is completed.
(6)苗木栽植:在4月下旬至5月上旬,选用规格为6cm×10cm的无纺布容器杯装填步骤(1)的培养基质,然后将填满培养基质的容器杯在苗圃地摆放整齐,容器杯间空隙用土填实,浇透水使容器杯中的培养基质沉实,在营养杯中用竹签插一个8cm深的小穴,再将嫁接好的油茶苗栽入杯中,向苗的方向回土,然后浇透水,小拱棚覆膜(覆膜方法:先每隔1m用竹片支起棚弓径,然后盖农用薄膜,再用土将四周压紧,防止空气对流);(6) Seedling planting: From late April to early May, select a non-woven container cup with a specification of 6cm×10cm to fill the culture medium of step (1), and then place the container cup filled with the culture medium in the nursery Neatly, fill the space between the container cups with soil, water thoroughly to make the culture medium in the container cup heavy, insert a small hole 8cm deep with a bamboo stick in the nutrition cup, and then plant the grafted Camellia oleifera seedlings into the cup, and place the seedlings in the cup. Return to the soil in the direction of the soil, then pour water thoroughly, and cover the small arch shed with film (the method of covering the film: first use bamboo pieces to support the bow diameter of the shed every 1m, then cover the agricultural film, and then press the surrounding area with soil to prevent air convection);
(7)架设荫棚:在栽植油茶嫁接苗的苗圃地架设荫棚,荫棚高:1.8m,棚株间距:4m,棚面用铁丝横拉作径,再覆盖遮阳网,并扎紧;(7) set up shade shed: set up shade shed at the nursery ground of planting camellia oleifera grafted seedlings, shade shed height: 1.8m, shed plant spacing: 4m, shed surface is drawn horizontally with iron wire as diameter, covers sunshade net again, and fastens;
(8)种植后管理:嫁接苗在膜内的时间为40d(以10%抽稍为准),揭膜前,遮荫严实,苗田不积水,揭膜后进行追肥,追肥时间为7~10月,每半月一次,以叶面喷洒方式实施(追肥所施的肥料配比为N2:K2O5:K2O=1:1:1,配成0.4~0.5%水溶液),同时每月喷洒含有效成分2%多菌灵水溶液和0.05%的生长素水溶液;及时除草,并进行修剪,剪除萌芽条,抹除花芽,留床苗,第二年在苗高30cm处摘顶,促进分枝;(8) Management after planting: the time for grafted seedlings to stay in the film is 40 days (subject to 10% pumping). Once every half month, it is implemented by spraying on the leaves (the fertilizer ratio of topdressing is N 2 : K 2 O 5 : K 2 O=1:1:1, made into 0.4-0.5% aqueous solution), and every Monthly spray 2% carbendazim aqueous solution and 0.05% auxin aqueous solution containing active ingredients; weeding in time, and pruning, cutting off the sprouting strips, erasing flower buds, leaving bed seedlings, and picking the top at a seedling height of 30cm in the second year to promote branch;
嫁接苗生长期间,环境温度控制在35℃以下,土壤始终保持湿润,空气湿度保持在80%,幼苗生长前期,严格控制光照条件,透光率在30%,随着幼苗生长和光照强度下降,到9月上旬以后,逐步增加透光量,10月中旬后,全光照,到了秋冬季,为了防止初霜影响,可以用遮阳网进行覆盖。During the growth of grafted seedlings, the ambient temperature is controlled below 35°C, the soil is always kept moist, and the air humidity is kept at 80%. In the early stage of seedling growth, light conditions are strictly controlled, and the light transmittance is 30%. As the seedlings grow and the light intensity decreases, After the first ten days of September, gradually increase the amount of light transmission. After mid-October, it will be fully illuminated. In autumn and winter, in order to prevent the impact of the first frost, it can be covered with sunshade nets.
对本实施例的育苗结果进行统计,结果见表1:The seedling raising result of present embodiment is counted, and the results are shown in Table 1:
表1Table 1
由表1可以看出,当年生油茶幼苗苗高在15cm以上,地径超过0.25cm,且差异不显著,嫁接成苗率在95以上,出圃率在90%以上,效果非常明显。It can be seen from Table 1 that the seedling height of raw camellia oleifera seedlings was more than 15cm, and the ground diameter was more than 0.25cm, and the difference was not significant.
实施例2Example 2
2017年在河南省光山县文殊乡育苗基地实施,嫁接苗品种为长林3号、4号、18号、40号和53号,方法与实施例1相同,5月上中旬开始实施嫁接,连续两年,于当年12月底进行测定,调查发现:当年生油茶幼苗苗高在18cm以上,地径超过2.5cm,且差异不显著,嫁接成苗率在95%以上,出圃率在90%以上,效果非常明显。In 2017, it was implemented in the seedling breeding base of Wenshu Township, Guangshan County, Henan Province. The grafted seedling varieties were Changlin No. 3, No. 4, No. 18, No. 40 and No. 53. Two years later, measurements were made at the end of December of that year, and the survey found that: the height of the raw Camellia oleifera seedlings was more than 18cm, the ground diameter was more than 2.5cm, and the difference was not significant, the grafting rate was more than 95%, and the emergence rate was more than 90%. The effect is very obvious.
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CN110583269A (en) * | 2019-10-22 | 2019-12-20 | 海南大学 | Production method of scion for grafting camellia oleifera in Hainan region |
CN111328567A (en) * | 2020-02-26 | 2020-06-26 | 株洲市地杰现代农业有限责任公司 | Camellia oleifera seedling breeding method |
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CN103283450A (en) * | 2013-05-24 | 2013-09-11 | 广西师范大学 | Golden camellia bud seedling stock grafting propagation method |
CN103460943A (en) * | 2013-09-18 | 2013-12-25 | 安顺市纳阳生态农业发展有限公司 | Elite breeding method for tea-oil trees |
CN104255309A (en) * | 2014-09-16 | 2015-01-07 | 锦屏县绿源种植有限公司 | Method for grafting oil tea seedling |
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