CN108387428A - A kind of colouring method of pathological section - Google Patents
A kind of colouring method of pathological section Download PDFInfo
- Publication number
- CN108387428A CN108387428A CN201810159656.6A CN201810159656A CN108387428A CN 108387428 A CN108387428 A CN 108387428A CN 201810159656 A CN201810159656 A CN 201810159656A CN 108387428 A CN108387428 A CN 108387428A
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- Prior art keywords
- pathological section
- reagent
- colouring method
- reaction reagent
- oil film
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- 238000004040 coloring Methods 0.000 title claims abstract description 30
- 230000001575 pathological effect Effects 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 29
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 70
- 238000006243 chemical reaction Methods 0.000 claims abstract description 45
- 238000004043 dyeing Methods 0.000 claims abstract description 21
- 238000011534 incubation Methods 0.000 claims abstract description 7
- 239000011521 glass Substances 0.000 claims description 23
- 239000007788 liquid Substances 0.000 claims description 6
- 239000004615 ingredient Substances 0.000 claims description 5
- 230000003252 repetitive effect Effects 0.000 claims description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 3
- 239000004215 Carbon black (E152) Substances 0.000 claims description 3
- 229910052799 carbon Inorganic materials 0.000 claims description 3
- 229930195733 hydrocarbon Natural products 0.000 claims description 3
- 150000002430 hydrocarbons Chemical class 0.000 claims description 3
- 201000010099 disease Diseases 0.000 claims 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims 1
- 238000010186 staining Methods 0.000 abstract description 5
- 230000000694 effects Effects 0.000 description 5
- 230000003118 histopathologic effect Effects 0.000 description 4
- 238000011532 immunohistochemical staining Methods 0.000 description 2
- 238000010339 medical test Methods 0.000 description 2
- WZUVPPKBWHMQCE-XJKSGUPXSA-N (+)-haematoxylin Chemical compound C12=CC(O)=C(O)C=C2C[C@]2(O)[C@H]1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-XJKSGUPXSA-N 0.000 description 1
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Natural products C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 1
- 238000007259 addition reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 description 1
- 230000009969 flowable effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a kind of colouring methods of pathological section, are applied to automation coloring system, are included in dyeing course, the step of reaction reagent stationary incubation 1 carries out mixing to reaction reagent after ten minutes is added.Technical scheme of the present invention improves staining pathologic section method, and mixing reaction reagent during incubation improves the service efficiency of reagent, saves the dosage of reagent.
Description
Technical field
The present invention relates to the technical field of medical test more particularly to a kind of colouring methods of histopathologic slide.
Background technology
Staining pathologic section is the important means in medical test, and current colouring method includes normal dyeing, IHC dyes
Color, specific stain etc. need to use various reagents.In existing colouring method, during being incubated after addition reaction reagent, one
As can keep standing glass slide, cause fully participate in reacting from tissue reaction reagent farther out in this way, cause the wave of reagent
Take.If can thus make to also assist in immune response from the reaction reagent of tissue farther out, the service efficiency of reagent will be improved.It is existing
Staining Protocol is automated, reagent volatilizees in order to prevent when dyeing, increases lid or addition fluid oil on glass slide sometimes
Film.Load fragment and lid are placed on the specific pallet of dyeing instrument, and lid is in addition to that can prevent volatilization from can also keep tissue
The temperature of reagent is stablized, and coloring is enhanced.Similarly, liquid oil film also plays the effect for preventing volatilization.But such as preceding institute
It states, in order to make tissue fully be covered by reagent, when dyeing usually requires all to cover entire glass slide into reagent, a large amount of to try
Agent cannot participate in reaction, cause unnecessary waste.If all reagents can be made to be involved in reaction, reagent will be greatlyd save
Dosage.
Invention content
The purpose of the present invention is to provide a kind of colouring methods of histopathologic slide, and reagent makes when reducing dyeing
Dosage improves the service efficiency of reagent.To achieve the goals above, the present invention provides the following technical solutions：A kind of pathological section
Colouring method, be applied to automation coloring system, including step：
B, reaction reagent is added to pathological section；
C, stationary incubation 1-10 minutes；
D, after completing step c, mixing is carried out to reaction reagent；
Preferably, further include step after step d：E, after completing step d, step c, step d are at least repeated once
Operation.
Preferably, glass slide lid is used in dyeing course；The glass slide lid includes reaction chamber, loading slot and dashes forward
The portion of rising；Glass slide lid is positioned in automation dyeing instrument pallet, and the reaction chamber position of lid corresponds to the tissue on glass slide and cuts
Piece position.
Preferably, the step b is specifically included：Reaction reagent is added in lid loading slot.
Preferably, the step d is specifically included：Cover panel is lifted and puts back to origin-location again, stands glass slide
Afterwards, repetitive operation so that reaction reagent mixes well.
Preferably, the step d is specifically included：Cover panel is lifted and will be put again after being dragged to protruding portion direction
Origin-location is put back into, after standing glass slide, repetitive operation so that reaction reagent mixes well.
Preferably, liquid oil film is used in dyeing course；The oil film is wrapped in outside pathological section；Oil film ingredient be containing
Carbon number is the hydrocarbon of C10-C16.
Preferably, the step b is specifically included：Reagent is added to pathological section, reagent connects across oil film with pathological section
It touches, oil film is wrapped in outside reaction reagent.
Preferably, the step d is specifically included：Air-flow is provided to the reaction reagent that is wrapped up by oil film, under the action of air-flow
Reaction reagent flows within the scope of oil film；After standing, repetitive operation so that reaction reagent mixes well.
Preferably, the oil film ingredient is C15H32。
Technical scheme of the present invention improves staining pathologic section method, mixing reaction reagent during incubation, improves
The service efficiency of reagent saves total dosage of reagent.
Specific implementation mode
To make technical staff more fully understand the present invention, the embodiment of the present invention is clearly and detailedly said below
It is bright but not as a limitation of the invention.Obviously, described embodiment is only a part of the embodiment of the present invention, without
It is whole embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are not before making creative work
The every other embodiment obtained is put, shall fall within the protection scope of the present invention.
In one embodiment, histopathologic slide's colouring method disclosed by the invention, includes the following steps：
(1) glass slide for posting pathological section is placed in automation dyeing instrument pallet；
(2) glass slide lid is positioned on glass slide, the reaction chamber position of lid corresponds to the histotomy on glass slide
Position；
(3) reaction reagent is added in lid loading slot；
(4) mixing reaction reagent：After reagent is incubated 1-10 minutes, cover panel is lifted and puts back to origin-location again, or
Person lifts cover panel will be placed back into origin-location again after the dragging of protruding portion direction；
(5) it after standing glass slide, repeats step (4) and operates so that reaction reagent mixes well.
Above-mentioned the step of repeating mixing so that it is originally separate to organize, have neither part nor lot in the reagent of reaction to be moved to above tissue,
It is reacted with tissue, improves the service efficiency of reagent, under the premise of reaching effect same, save the dosage of reagent.With
For Ki67 dyeing, the antibody reagent dosage about 100ul of existing colouring method needs；Using the glass slide lid of the present invention
When carrying out Ki67 dyeing with above-mentioned colouring method, it is only 70ul to reach antibody reagent dosage needed for same coloring.Similarly
, in other a variety of dyeing, 20% or more can be reduced using amount of reagent after the above method, is greatly saved and is coloured to
This.
In one embodiment, staining pathologic section method disclosed by the invention, includes the following steps：
(a) glass slide for posting pathological section is placed in automation dyeing instrument pallet；
(b) it is C ingredient to be added dropwise to histopathologic slide position15H32Liquid oil film；
(c) reagent is added to pathological section, reagent is contacted across oil film with pathological section, and oil film is wrapped in outside reagent；
(d) mixing reaction reagent：After reagent is incubated 1-10 minutes, air-flow, gas are provided to the reaction reagent wrapped up by oil film
The effect of stream makes reaction reagent be flowed within the scope of oil film；Or by rocking glass slide so that reaction reagent is flowable,
It has reached unreacted reagent and flow to above pathological section the effect for participating in reaction.
(e) after standing, repeat the above steps (d) operation 1-3 times so that reaction reagent mixes well.
The above-mentioned colouring method using liquid oil film carries out mixing during incubation to reaction reagent so that original reaction
Reagent must participate in greatest extent reaction, improve the service efficiency of reagent, under the premise of reaching effect same, save reagent
Dosage.The liquid oil film that above-mentioned colouring method is applied can also be other containing the hydrocarbon that carbon number is C10-C16.
In the colouring method of the present invention, specific incubation time is depending on specific reaction reagent.The colouring method of the present invention
Applied to immunohistochemical staining, normal dyeing and specific stain.In immunohistochemical staining, primary antibody is incubated, secondary antibody is incubated, DAB
Dyeing etc. can apply the operating method of the mixing reaction reagent disclosed in the technical program；Similarly, technical side of the invention
Case can be applied in the normal dyeings such as eosin stains, haematoxylin dyeing.
The foregoing is merely the preferred embodiment of the present invention, are not intended to limit the scope of the invention, every at this
Under the inventive concept of invention, is extended using technology made by description of the present invention or recreation is included in the special of the present invention
In sharp protection domain.
Claims (10)
1. a kind of colouring method of pathological section is applied to automation coloring system, which is characterized in that including step：
B, reaction reagent is added to pathological section；
C, stationary incubation 1-10 minutes；
D, after completing step c, mixing is carried out to reaction reagent.
2. the colouring method of pathological section according to claim 1, which is characterized in that after step d further include step：
E, after completing step d, step c, step d operations are at least repeated once.
3. the colouring method of pathological section according to claim 2, which is characterized in that use glass slide lid in dyeing course
Body；The glass slide lid includes reaction chamber, loading slot and protrusion；Glass slide lid is positioned over automation dyeing instrument pallet
Interior, the reaction chamber position of lid corresponds to the histotomy position on glass slide.
4. the colouring method of pathological section according to claim 3, which is characterized in that the step b is specifically included：In lid
Reaction reagent is added in loading slot.
5. the colouring method of pathological section according to claim 4, which is characterized in that the step d is specifically included：By lid
Protruding portion lifts and puts back to origin-location again, after standing glass slide, repetitive operation so that reaction reagent mixes well.
6. the colouring method of pathological section according to claim 4, which is characterized in that the step d is specifically included：By lid
Protruding portion lifts and will be placed back into origin-location again after being dragged to protruding portion direction, after standing glass slide, repetitive operation so that anti-
Reagent is answered to mix well.
7. the colouring method of pathological section according to claim 2, which is characterized in that use liquid oil film in dyeing course；
The oil film is wrapped in outside pathological section；Oil film ingredient be C10-C16 containing carbon number hydrocarbon.
8. the colouring method of pathological section according to claim 7, which is characterized in that the step b is specifically included：To disease
Reagent is added in reason slice, and reagent is contacted across oil film with pathological section, and oil film is wrapped in outside reaction reagent.
9. the colouring method of pathological section according to claim 7, which is characterized in that the step d is specifically included：To by oil
The reaction reagent of film package provides air-flow, and reaction reagent flows within the scope of oil film under the action of air-flow；After standing, repeat to grasp
Make so that reaction reagent mixes well.
10. the colouring method of pathological section according to claim 7, which is characterized in that the oil film ingredient is C15H32。
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810159656.6A CN108387428A (en) | 2018-02-26 | 2018-02-26 | A kind of colouring method of pathological section |
| PCT/CN2018/103399 WO2019161653A1 (en) | 2018-02-26 | 2018-08-31 | Method for staining pathological sections |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810159656.6A CN108387428A (en) | 2018-02-26 | 2018-02-26 | A kind of colouring method of pathological section |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108387428A true CN108387428A (en) | 2018-08-10 |
Family
ID=63069279
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810159656.6A Pending CN108387428A (en) | 2018-02-26 | 2018-02-26 | A kind of colouring method of pathological section |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN108387428A (en) |
| WO (1) | WO2019161653A1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109238812A (en) * | 2018-10-26 | 2019-01-18 | 天津源天晟科技发展有限公司 | A kind of automatic staining device and its control method |
| WO2019161653A1 (en) * | 2018-02-26 | 2019-08-29 | 深圳达盟生物科技有限公司 | Method for staining pathological sections |
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| US20020001849A1 (en) * | 1990-03-02 | 2002-01-03 | Copeland Keith G. | Automated biological reaction apparatus |
| CN1672030A (en) * | 2002-06-20 | 2005-09-21 | 视觉生物体系有限公司 | Microscope slide cover with integrated reservoir |
| US20060019302A1 (en) * | 2004-07-23 | 2006-01-26 | Charles Lemme | Method and apparatus for applying fluids to a biological sample |
| CN101203598A (en) * | 2005-04-21 | 2008-06-18 | 塞莱勒斯诊断公司 | Enhanced fluidic method and apparatus for automated rapid immunohistochemistry |
| CN104094122A (en) * | 2011-11-16 | 2014-10-08 | 莱卡生物系统墨尔本私人有限公司 | Cover member, method and treatment module for treating biological sample on substrate |
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| CN102266386B (en) * | 2010-06-01 | 2013-05-01 | 四川大千药业有限公司 | Scutellaria baicalensis extract and preparation method and uses thereof |
| CN202204717U (en) * | 2011-08-18 | 2012-04-25 | 浙江世纪康大医疗科技有限公司 | Pathological section covering plate of staining instrument |
| JP6237194B2 (en) * | 2013-12-18 | 2017-11-29 | コニカミノルタ株式会社 | Dyeing method |
| CN103698507B (en) * | 2014-01-09 | 2015-05-20 | 南京医科大学第一附属医院 | Detachable and sealed space glass slide incubator |
| CN108387428A (en) * | 2018-02-26 | 2018-08-10 | 深圳安诺康生物医疗有限公司 | A kind of colouring method of pathological section |
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2018
- 2018-02-26 CN CN201810159656.6A patent/CN108387428A/en active Pending
- 2018-08-31 WO PCT/CN2018/103399 patent/WO2019161653A1/en active Application Filing
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020001849A1 (en) * | 1990-03-02 | 2002-01-03 | Copeland Keith G. | Automated biological reaction apparatus |
| CN1672030A (en) * | 2002-06-20 | 2005-09-21 | 视觉生物体系有限公司 | Microscope slide cover with integrated reservoir |
| US20060019302A1 (en) * | 2004-07-23 | 2006-01-26 | Charles Lemme | Method and apparatus for applying fluids to a biological sample |
| CN101203598A (en) * | 2005-04-21 | 2008-06-18 | 塞莱勒斯诊断公司 | Enhanced fluidic method and apparatus for automated rapid immunohistochemistry |
| CN104094122A (en) * | 2011-11-16 | 2014-10-08 | 莱卡生物系统墨尔本私人有限公司 | Cover member, method and treatment module for treating biological sample on substrate |
| CN206799660U (en) * | 2017-04-12 | 2017-12-26 | 河南赛诺特生物技术有限公司 | Nucleic acid hybridization slide |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019161653A1 (en) * | 2018-02-26 | 2019-08-29 | 深圳达盟生物科技有限公司 | Method for staining pathological sections |
| CN109238812A (en) * | 2018-10-26 | 2019-01-18 | 天津源天晟科技发展有限公司 | A kind of automatic staining device and its control method |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2019161653A1 (en) | 2019-08-29 |
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| TA01 | Transfer of patent application right |
Effective date of registration: 20180809 Address after: 518000 nine, 901 C, Jinwei Yuan Industrial Park, poly dragon hill area, Pingshan District, Shenzhen, Guangdong. Applicant after: Shenzhen Da Meng Biotechnology Co., Ltd. Applicant after: HENAN CELLNOVO BIOTECHNOLOGY CO., LTD. Address before: 518000 Guangdong Shenzhen Baoan District Fuyong Street Peace community Junfeng industrial complex building B5-1 block A block 201C Applicant before: Shenzhen Annuo Kang biological medical Co Ltd |
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Application publication date: 20180810 |
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