CN108377842A - A kind of implantation methods of black fungus - Google Patents

A kind of implantation methods of black fungus Download PDF

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Publication number
CN108377842A
CN108377842A CN201810461498.XA CN201810461498A CN108377842A CN 108377842 A CN108377842 A CN 108377842A CN 201810461498 A CN201810461498 A CN 201810461498A CN 108377842 A CN108377842 A CN 108377842A
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China
Prior art keywords
parts
bacteria stick
black fungus
culture substrate
culture
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CN201810461498.XA
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Chinese (zh)
Inventor
曾平
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Wuchuan County Hui Farmers Modern Agricultural Development Co Ltd
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Wuchuan County Hui Farmers Modern Agricultural Development Co Ltd
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Priority to CN201810461498.XA priority Critical patent/CN108377842A/en
Publication of CN108377842A publication Critical patent/CN108377842A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/20Culture media, e.g. compost
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D1/00Fertilisers containing potassium
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Inorganic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Mushroom Cultivation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

This application discloses a kind of implantation methods of black fungus of agricultural plantation technology field, include the following steps:Step 1: making culture substrate:Prepare in parts by weight:3~5 parts of 50~60 parts of sawdust, 35~40 parts of bagasse, 12~17 parts of food leaf grass, 5~8 parts of Compositions of Bamboo Shoot Shell, 4~6 parts of cotton seed hulls, 2.5~3 parts of lime, 10~13 parts of plant ash and water, obtain culture substrate after mixing;Step 2: pack:It is packed into 1000~1200g of culture substrate in parts by weight after culture bag sterilizing, in each culture bag and bacteria stick is made;Step 3: implantation strain:In bacteria stick acanthopore, and by black fungus bacterial kind implant holes, then bacteria stick is put into the culturing room that temperature is 23~27 DEG C again and is cultivated;Step 4: management:Moisture supplement was carried out to the bacteria stick in culturing room in every 3~5 days, the water content of bacteria stick is 62%~64%, after mycelia grows, carries out moisture supplement to bacteria stick within every 4~6 days, the water content of bacteria stick is 73%~76%.It is good using the black fungus yield height of the application plantation, quality.

Description

A kind of implantation methods of black fungus
Technical field
The invention belongs to agricultural plantation technology fields, and in particular to a kind of implantation methods of black fungus.
Background technology
Black fungus is a kind of edible mushroom full of nutrition, and is the traditional health food in China and exporting.It other There are many name, because being grown on rotten wood, are similar to the ear of people, therefore named agaric;It is again slim and graceful like moth butterfly, also known as the wooden moth;Because it Taste is delicious just like chicken, therefore also name tree chicken, the wooden machine (it is machine that Gu Nanchu people, which calls chicken), and the agaric of polyphyll is inlayed mutually in the tree, Just like piece piece floating clouds, and have the title of cloud ear.
Traditional fungus cultivation i.e. use is segment wood cultivated, and cultivation flow includes mainly:Place election, strain preparation, Prepare Duan Mu, inoculation and field planting management step.But the agaric low output of this segment wood cultivated method, cannot be satisfied the market demand. With the popularization and development of artificial cultivation technique, the experimental study of bag cultivating method agaric new technology has further been pushed.Plastic bag cultivation is black Agaric is will to be packed into special high-temperature resistance plastice bag by the culture material of the material compositions such as cotton seed hulls, sawdust, corncob, straw, wheat straw In, by high temperature sterilization, inoculation, culture, scarfing produce agaric and etc. cultivate black fungus.Currently, in most black fungus plantation, The most of weed tree sawdust for being all use of its culture substrate is as main matrix, but weed tree sawdust nutritional ingredient is relatively low, and plantation comes out Black fungus, not only poor quality, and the problems such as there are low output and unstable yield.
Invention content
The invention is intended to provide a kind of implantation methods of black fungus, to solve prior art plantation black fungus poor quality and production Measure low problem.
In order to solve the above technical problems, the present invention provides the following technical solutions:A kind of implantation methods of black fungus, including with Lower step:
Step 1: making culture substrate:Prepare in parts by weight:50~60 parts of sawdust, 35~40 parts of bagasse, food leaf grass 3~5 parts of 12~17 parts, 5~8 parts of Compositions of Bamboo Shoot Shell, 4~6 parts of cotton seed hulls, 2.5~3 parts of lime, 10~13 parts of plant ash and water, mixing Culture substrate is obtained after uniformly;
Step 2: pack:After culture bag sterilizing, culture substrate 1000 is packed into each culture bag in parts by weight Simultaneously bacteria stick is made in~1200g;
Step 3: implantation strain:In bacteria stick acanthopore, and by black fungus bacterial kind implant holes, bacteria stick is then put into temperature again It is cultivated in the culturing room that degree is 23~27 DEG C;
Step 4: management:Moisture supplement being carried out to the bacteria stick in culturing room in every 3~5 days, the water content of bacteria stick is 62%~ 64%, after mycelia grows, moisture supplement was carried out to bacteria stick in every 4~6 days, the water content of bacteria stick is 73%~76%.
Beneficial effects of the present invention:
The implantation methods of black fungus in this programme, on the basis of existing culture substrate, by the way that bagasse, food leaf is added Grass, Compositions of Bamboo Shoot Shell, cotton seed hulls, lime and plant ash, because the nutrient of black fungus growth is mainly with carbohydrate and nitrogen substance Based on and a small amount of inorganic salts, and it is a kind of natural plant protein resource to eat leaf grass, is rich in protein, can thus give Black fungus provides abundant nitrogen source, contains sucrose in bagasse, after being put into water mixing, can obtain the carbon hydrate of solvable state Object after the mycelia of black fungus absorbs, secretes a large amount of enzyme and then cellulose in sawdust, Compositions of Bamboo Shoot Shell and cotton seed hulls and wooden The carbohydrate breakdowns such as element give full play to the raw material in culture substrate at soluble object sugar, needed for mycelia supplement Nutrition;It is the weight of protein and enzyme in black fungus again to contain mineral, these minerals such as potassium, calcium and iron in lime and plant ash Enzyme and the decomposition for cellulose and lignin, original each in this way are converted it into after wanting constituent, black fungus mycelia to absorb again Mutually using being complementary to one another between material, and then the growth and development of black fungus mycelia are promoted, and because black fungus belongs to medium temperature Type mushroom promotes the growth of mycelia, then again to bacteria stick again in this way so being cultivated at a temperature of mycelia is placed on 23~27 DEG C On mycelia be managed because implantation strain after, the growth of black fungus includes mainly agaric mycelium and fructification, and The auricle of black fungus be colloid absorption moisture after expand and could develop, therefore, after mycelia grows, every 4~6 days bacterium bacteria sticks carry out Moisture supplements, and the water content of bacteria stick is 73%~76%, and thereby promoting the growths of black fungus.Compared with prior art and It says, the raw material in this programme is easy to get, and mutually promotes, and then the nutrition for giving black fungus supplement sufficient, in combination with rational Management, thus substantially increases the quality and yield of black fungus.
Further, 15~20h is fumigated using potassium permanganate when culture bag sterilizing in the step 2.By with height The stifling mode of potassium manganate can effectively kill the bacterium in packaging bag, and fumigation time is 15~20h, thus can be complete The bacterium in packaging bag is killed entirely, safe growing environment is provided to the growth of strain.
Further, culturing room is aerated in the step 4, divulge information 1~2h daily.Because black fungus is a kind of good Gas fungi needs sufficient oxygen, therefore the 1~2h that divulges information daily, avoids the too high levels of culture indoor carbon dioxide, Ensure that mycelium in bacteria stick can normal growth, improve the yield of black fungus.
Specific implementation mode
It is further described below by specific implementation mode:
Embodiment 1:
A kind of implantation methods of black fungus, include the following steps:
Step 1: making culture substrate:Prepare in parts by weight:50 parts of sawdust, 35 parts of bagasse, food leaf careless 12 parts, bamboo 3 parts of 5 parts of bamboo shoot shell, 4 parts of cotton seed hulls, 2.5 parts of lime, 10 parts of plant ash and water, obtain culture substrate after mixing;
Step 2: pack:After culture bag sterilizing, it is packed into culture substrate in parts by weight in each culture bag Simultaneously bacteria stick is made in 1000g;
Step 3: implantation strain:In bacteria stick acanthopore, and by black fungus bacterial kind implant holes, bacteria stick is then put into temperature again It is cultivated in the culturing room that degree is 23 DEG C;
Step 4: management:Moisture supplement was carried out to the bacteria stick in culturing room in every 3 days, the water content of bacteria stick is 62%, mycelia After growing, moisture supplement was carried out to bacteria stick in every 4 days, the water content of bacteria stick is 73%.
Embodiment 2:
A kind of implantation methods of black fungus, include the following steps:
Step 1: making culture substrate:Prepare in parts by weight:55 parts of sawdust, 37 parts of bagasse, food leaf careless 14 parts, bamboo 4 parts of 6 parts of bamboo shoot shell, 5 parts of cotton seed hulls, 2.7 parts of lime, 12 parts of plant ash and water, obtain culture substrate after mixing;
Step 2: pack:After culture bag sterilizing, it is packed into culture substrate in parts by weight in each culture bag Simultaneously bacteria stick is made in 1100g;
Step 3: implantation strain:In bacteria stick acanthopore, and by black fungus bacterial kind implant holes, bacteria stick is then put into temperature again It is cultivated in the culturing room that degree is 24 DEG C;
Step 4: management:Moisture supplement was carried out to the bacteria stick in culturing room in every 4 days, the water content of bacteria stick is 63%, mycelia After growing, moisture supplement was carried out to bacteria stick in every 5 days, the water content of bacteria stick is 74%.
Embodiment 3:
A kind of implantation methods of black fungus, include the following steps:
Step 1: making culture substrate:Prepare in parts by weight:60 parts of sawdust, 40 parts of bagasse, food leaf careless 17 parts, bamboo 5 parts of 8 parts of bamboo shoot shell, 6 parts of cotton seed hulls, 3 parts of lime, 13 parts of plant ash and water, obtain culture substrate after mixing;
Step 2: pack:After culture bag sterilizing, it is packed into culture substrate in parts by weight in each culture bag Simultaneously bacteria stick is made in 1200g;
Step 3: implantation strain:In bacteria stick acanthopore, and by black fungus bacterial kind implant holes, bacteria stick is then put into temperature again It is cultivated in the culturing room that degree is 27 DEG C;
Step 4: management:Moisture supplement was carried out to the bacteria stick in culturing room in every 5 days, the water content of bacteria stick is 64%, mycelia After growing, moisture supplement was carried out to bacteria stick in every 6 days, the water content of bacteria stick is 76%.
Method and the prior art shown in Examples 1 to 3 are utilized respectively using weed tree sawdust as main matrix (comparative example) Black fungus is planted, black fungus is harvested after 1 year respectively and growth performance and product form is detected, as a result such as following table institute Show:
As can be known from the above table, the black fungus planted using the present invention not only increases yield simultaneously compared to existing technologies Also improve the speed of growth of black fungus.
Black fungus after obtained black fungus and comparative example are planted 1 year after being planted 1 year to Examples 1 to 3 method respectively It carries out endoplasm content to be detected, the material result contained in every 200 grams of black fungus is as shown in the table:
As can be known from the above table, the content for black fungus its nutriment planted using method is apparently higher than prior art kind Obtained black fungus is planted, the quality of black fungus is effectively improved.

Claims (3)

1. a kind of implantation methods of black fungus, it is characterised in that:Include the following steps:
Step 1: making culture substrate:Prepare in parts by weight:50~60 parts of sawdust, 35~40 parts of bagasse, food leaf grass 12~ 3~5 parts of 17 parts, 5~8 parts of Compositions of Bamboo Shoot Shell, 4~6 parts of cotton seed hulls, 2.5~3 parts of lime, 10~13 parts of plant ash and water are uniformly mixed After obtain culture substrate;
Step 2: pack:Be packed into parts by weight after culture bag sterilizing, in each culture bag culture substrate 1000~ Simultaneously bacteria stick is made in 1200g;
Step 3: implantation strain:It is in bacteria stick acanthopore, and by black fungus bacterial kind implant holes, bacteria stick is then put into temperature again It is cultivated in 23~27 DEG C of culturing room;
Step 4: management:Moisture supplement being carried out to the bacteria stick in culturing room in every 3~5 days, the water content of bacteria stick is 62%~ 64%, after mycelia grows, moisture supplement was carried out to bacteria stick in every 4~6 days, the water content of bacteria stick is 73%~76%.
2. the implantation methods of black fungus according to claim 1, it is characterised in that:Culture bag sterilization in the step 2 15~20h is fumigated using potassium permanganate when disinfection.
3. the implantation methods of black fungus according to claim 2, it is characterised in that:Culturing room is carried out in the step 4 Ventilation, divulge information 1~2h daily.
CN201810461498.XA 2018-05-15 2018-05-15 A kind of implantation methods of black fungus Pending CN108377842A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109937803A (en) * 2019-04-28 2019-06-28 蔡世荣 A method of hamlessizing technology black fungus is carried out using bacteria stick stem
CN117502107A (en) * 2023-11-28 2024-02-06 喀什鲁疆情农业科技发展有限公司 A kind of culture material for planting black fungus using poplar sawdust and its preparation method and application
CN119032794A (en) * 2024-09-18 2024-11-29 山东航空学院 Edible fungus culture medium containing phyllanthus pratensis and cultivation method of high-quality edible fungus

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105961030A (en) * 2016-06-22 2016-09-28 潘雪玉 Black fungus cultivation method
CN106258469A (en) * 2015-05-18 2017-01-04 唐秀克 A kind of cultivation of auricularia auricula method
CN106747794A (en) * 2016-12-08 2017-05-31 河池市农业科学研究所 A kind of black fungus selenium-enriching cultivation method
CN106804284A (en) * 2015-11-30 2017-06-09 姜蕾 Black fungus breeding method

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106258469A (en) * 2015-05-18 2017-01-04 唐秀克 A kind of cultivation of auricularia auricula method
CN106804284A (en) * 2015-11-30 2017-06-09 姜蕾 Black fungus breeding method
CN105961030A (en) * 2016-06-22 2016-09-28 潘雪玉 Black fungus cultivation method
CN106747794A (en) * 2016-12-08 2017-05-31 河池市农业科学研究所 A kind of black fungus selenium-enriching cultivation method

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
燕红等: "黑木耳栽培技术", 《微生物资源及利用》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109937803A (en) * 2019-04-28 2019-06-28 蔡世荣 A method of hamlessizing technology black fungus is carried out using bacteria stick stem
CN109937803B (en) * 2019-04-28 2023-11-24 蔡世荣 Method for harmless production of black fungus by using fungus stick core column
CN117502107A (en) * 2023-11-28 2024-02-06 喀什鲁疆情农业科技发展有限公司 A kind of culture material for planting black fungus using poplar sawdust and its preparation method and application
CN119032794A (en) * 2024-09-18 2024-11-29 山东航空学院 Edible fungus culture medium containing phyllanthus pratensis and cultivation method of high-quality edible fungus

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