CN108368523A - 在代谢工程化的乳酸菌中高水平生产双乙酰 - Google Patents

在代谢工程化的乳酸菌中高水平生产双乙酰 Download PDF

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CN108368523A
CN108368523A CN201680071433.3A CN201680071433A CN108368523A CN 108368523 A CN108368523 A CN 108368523A CN 201680071433 A CN201680071433 A CN 201680071433A CN 108368523 A CN108368523 A CN 108368523A
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Chinese (zh)
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克里斯汀·索利姆
彼得·鲁达尔·詹森
刘建民
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Danmarks Tekniskie Universitet
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Danmarks Tekniskie Universitet
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/20—Synthetic spices, flavouring agents or condiments
    • A23L27/24—Synthetic spices, flavouring agents or condiments prepared by fermentation
    • A23L27/25—Dairy flavours
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
    • C12P7/26—Ketones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/225—Lactobacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01004—R,R-butanediol dehydrogenase (1.1.1.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01027—L-Lactate dehydrogenase (1.1.1.27)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01028—D-Lactate dehydrogenase (1.1.1.28)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01303—Diacetyl reductase, [(R)-acetoin forming] (1.1.1.303)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
    • C12Y106/03—Oxidoreductases acting on NADH or NADPH (1.6) with oxygen as acceptor (1.6.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01069—Protein-Npi-phosphohistidine-sugar phosphotransferase (2.7.1.69), i.e. sucrose phosphotransferase system II
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01144—Tagatose-6-phosphate kinase (2.7.1.144)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01085—6-Phospho-beta-galactosidase (3.2.1.85)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/02—Aldehyde-lyases (4.1.2)
    • C12Y401/0204—Tagatose-bisphosphate aldolase (4.1.2.40)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01026—Galactose-6-phosphate isomerase (5.3.1.26)

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nutrition Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201680071433.3A 2015-10-09 2016-10-07 在代谢工程化的乳酸菌中高水平生产双乙酰 Pending CN108368523A (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
EP15189200.7 2015-10-09
EP15189200 2015-10-09
EP15194710 2015-11-16
EP15194710.8 2015-11-16
EP16157443.9 2016-02-25
EP16157443 2016-02-25
PCT/EP2016/074043 WO2017060455A1 (fr) 2015-10-09 2016-10-07 Production de haut niveau de diacétyle dans une bactérie lactique obtenue par génie métabolique

Publications (1)

Publication Number Publication Date
CN108368523A true CN108368523A (zh) 2018-08-03

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201680071433.3A Pending CN108368523A (zh) 2015-10-09 2016-10-07 在代谢工程化的乳酸菌中高水平生产双乙酰

Country Status (5)

Country Link
US (1) US10563271B2 (fr)
EP (1) EP3359678A1 (fr)
CN (1) CN108368523A (fr)
AU (1) AU2016335266A1 (fr)
WO (1) WO2017060455A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110713990A (zh) * 2019-10-21 2020-01-21 北京化工大学 一种烯酸还原酶的突变体蛋白及其应用
CN110897092A (zh) * 2019-11-29 2020-03-24 西华大学 一种阻断发酵食品中抗生素抗性基因水平转移的方法及其应用
CN111500614A (zh) * 2020-05-19 2020-08-07 江南大学 高效催化l-苏氨酸合成2,5-dmp的质粒及其构建与应用
WO2023155474A1 (fr) * 2022-02-15 2023-08-24 浙江大学杭州国际科创中心 Méso -2,3-butanediol déshydrogénase, et mutant et son utilisation

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11130973B1 (en) * 2020-05-25 2021-09-28 Cofco (Jilin) Bio-Chemical Technology Co., Ltd Recombinant strain for producing L-lactic acid

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102712912A (zh) * 2009-09-29 2012-10-03 布特马斯先进生物燃料有限责任公司 在乳酸菌中改善流向乙酰乳酸来源产物的流量
US20140113376A1 (en) * 2011-06-01 2014-04-24 Rotem Sorek Compositions and methods for downregulating prokaryotic genes

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2991458B2 (ja) 1990-05-31 1999-12-20 明治乳業株式会社 乳酸菌の増殖性及び抗菌性物質生産性の増大法
US6413765B1 (en) 1998-04-21 2002-07-02 Chr. Hanson A/S Genetically modified lactic acid bacteria having modified diacetyl reductase activities

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102712912A (zh) * 2009-09-29 2012-10-03 布特马斯先进生物燃料有限责任公司 在乳酸菌中改善流向乙酰乳酸来源产物的流量
US20140113376A1 (en) * 2011-06-01 2014-04-24 Rotem Sorek Compositions and methods for downregulating prokaryotic genes

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ANA PAULA OLIVEIRA等: ""Modeling Lactococcus lactis using a genome-scale flux model"", 《BMC MICROBIOLOGY》 *
MURIEL COCAIGN-BOUSQUET等: ""Anaerobic sugar catabolism in Lactococcus lactis: genetic regulation and enzyme control over pathway flux"", 《APPL MICROBIOL BIOTECHNOL》 *
付良: ""乳酸乳球菌的有氧呼吸研究"", 《中国优秀硕士学位论文全文数据库基础科学辑》 *
韩希妍等: ""乳酸风味代谢物质的基因调控"", 《微生物学报》 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110713990A (zh) * 2019-10-21 2020-01-21 北京化工大学 一种烯酸还原酶的突变体蛋白及其应用
CN110713990B (zh) * 2019-10-21 2021-08-10 北京化工大学 一种烯酸还原酶的突变体蛋白及其应用
CN110897092A (zh) * 2019-11-29 2020-03-24 西华大学 一种阻断发酵食品中抗生素抗性基因水平转移的方法及其应用
CN111500614A (zh) * 2020-05-19 2020-08-07 江南大学 高效催化l-苏氨酸合成2,5-dmp的质粒及其构建与应用
WO2023155474A1 (fr) * 2022-02-15 2023-08-24 浙江大学杭州国际科创中心 Méso -2,3-butanediol déshydrogénase, et mutant et son utilisation

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EP3359678A1 (fr) 2018-08-15
WO2017060455A1 (fr) 2017-04-13
US20180282827A1 (en) 2018-10-04
AU2016335266A1 (en) 2018-05-31
US10563271B2 (en) 2020-02-18

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Application publication date: 20180803