CN108362869A - A kind of incubation box - Google Patents

A kind of incubation box Download PDF

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Publication number
CN108362869A
CN108362869A CN201810375295.9A CN201810375295A CN108362869A CN 108362869 A CN108362869 A CN 108362869A CN 201810375295 A CN201810375295 A CN 201810375295A CN 108362869 A CN108362869 A CN 108362869A
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CN
China
Prior art keywords
box body
support element
support
box
lever
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201810375295.9A
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Chinese (zh)
Inventor
王丽杰
周洪辉
胡滨阳
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FUZHOU MAIXIN BIOLOGICAL TECHNOLOGY DEVELOPMENT Co Ltd
Original Assignee
FUZHOU MAIXIN BIOLOGICAL TECHNOLOGY DEVELOPMENT Co Ltd
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Application filed by FUZHOU MAIXIN BIOLOGICAL TECHNOLOGY DEVELOPMENT Co Ltd filed Critical FUZHOU MAIXIN BIOLOGICAL TECHNOLOGY DEVELOPMENT Co Ltd
Priority to CN201810375295.9A priority Critical patent/CN108362869A/en
Publication of CN108362869A publication Critical patent/CN108362869A/en
Pending legal-status Critical Current

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The invention discloses a kind of incubation boxes, including box body and box cover;The support component of at least one set of support glass slide is provided in the box body, the support component includes adjacent and horizontally disposed first support element and the second support element;First support element is the supporting rod that section is cam shape, and the end face of the supporting rod is fixedly installed lever, and the lever is movably arranged in outside box body, and the other end of supporting rod is flexibly connected with the one side wall of box body;The supporting rod can be axially rotationally set in box body between two side;The both ends of the surface of second support element are fixedly connected with two madial walls of box body respectively, and the second support element includes the first supporting surface and the second supporting surface, and second supporting surface is vertically connect with the first supporting surface.For the present invention by the way that the supporting rod that section is cam shape is arranged, the multiple glass slides set are discharged together in realization to have the advantages that simple operation with synchronous developing.

Description

A kind of incubation box
Technical field
The present invention relates to Pathological experiment instrument field more particularly to a kind of incubation boxes.
Background technology
Since cell or tissue living is mostly water white transparency, lack between intracellular various structures between various tissues Contrast is not easy clearly to distinguish under general light microscopic;In addition, tissue will soon dead and generation tissue corruption after leaving body It loses, loses original normal configuration, therefore, tissue will be through fixation, washing and dehydration, transparent, waxdip, embedding, slice and patch, de- Wax, dyeing, dehydration, transparent, mounting can also improve between various tissues and cell to avoid cell tissue death The identification of interior various structures, to recognize its morphosis.The method is paraffin method, is histology conven tional tabletting techniques In the subjects such as most widely used method and pathology and medical jurisprudence studying, observe and judge the shape of cell tissue The main method of state variation.
The dyeing needs of slice carry out in being incubated box, are required for being incubated some time to being sliced often to carry out once dyeing, It carries out needing to remove the preceding reagent once added before dyeing next time after the completion of being incubated clean, it is therefore desirable to will cut one by one The reagent that piece is once added out of incubation box before taking-up is rinsed with PBS buffers, then whipping glass slide is to remove excessive buffering Agent, then slice is put back to and is incubated in box to carry out the addition and incubation of reagent next time.Since dyeing needs to add a variety of differences Reagent, therefore above-mentioned step is also required to repeated multiple times, and such operating process is excessively cumbersome.
Invention content
For this reason, it may be necessary to a kind of incubation box be provided, to solve the operating process to the flushing of slice and rejection tablet in the prior art Excessively cumbersome problem.
To achieve the above object, a kind of incubation box, including box body and box cover are inventor provided;It is provided in the box body The support component of at least one set support glass slide, the support component are located at the top of box bottom, and the support component includes Adjacent and horizontally disposed first support element and the second support element;
First support element is the supporting rod that section is cam shape, and the end face of the supporting rod is fixedly installed operation Handle, the lever are movably arranged in outside box body, and the other end of supporting rod is flexibly connected with the one side wall of box body;It is described Supporting rod can be axially rotationally set in box body between two side;
The both ends of the surface of second support element are fixedly connected with two madial walls of box body respectively, and the second support element includes first Supporting surface and the second supporting surface, second supporting surface are vertically connect with the first supporting surface.
Further, the support component is provided at least two, passes through transmission belt between the lever of the first support element It is sequentially connected, a lever is connected with handle.
Further, further include bolt, the end face of the lever is provided with threaded rod, in the first support element both ends of the surface Portion offers bolt hole respectively, and the two side of the box body opens up hole respectively, and bolt and threaded rod are through at box body two respectively The hole of side wall, and it is threaded onto the threaded hole of the first support element both ends of the surface respectively.
Further, second support element offers liquid-leaking nozzle.
Further, it is provided with handle on the box cover.
Further, further include jet pipe, hose and rubber bottle, equipped with nozzle, the tube wall of pipe spray is opened for one end of the jet pipe Equipped with hole for water spraying;The jet pipe is set in box cover, and hole for water spraying is oppositely arranged with support component;The nozzle of jet pipe is pierced by box cover Outside, one end of hose is placed at nozzle, and the other end is placed on the bottle mouth position of rubber bottle.
Further, the material of the box body is transparent glass or plastics.
Further, the width of the box body is 5cm-30cm.
Further, the spacing of first support element and the second support element is 5cm-6cm.
It is different from the prior art, above-mentioned technical proposal can be used for placing and need to incubate by being provided with the box body of box cover The slice educated.The glass slide placed and be sliced can be supported by the way that support component is arranged in box body, and support component is located at box body The top of bottom surface so that glass slide is hanging, will not be submerged in for rinsing in the liquid of slice.By the way that support component is arranged to Adjacent and horizontally disposed first support element and the second support element, the both ends for supporting glass slide respectively.First support element It is arranged to the supporting rod that section is cam shape, and lever is arranged in the end face of supporting rod, lever is movably through at box again In vitro, the other end of support element is flexibly connected with the one side wall of box body again, and user can to support by controlling lever Bar axially moves, and the height of the one end of glass slide on supporting rod can change with the axial movement of supporting rod, therefore Glass slide can change from horizontality to heeling condition, then change from heeling condition under the continuous axial movement of supporting rod Horizontality is faded to, it, can be by controlling the operation being located at outside box body when needing to drain the excessive buffer on glass slide Handle makes glass slide change from horizontality to heeling condition, can also be located at box by control after draining excessive buffer External lever changes glass slide to horizontality from heeling condition, to carry out flushing next time.By by second The both ends of the surface of support member are fixedly connected with two madial walls of box body respectively so that the second support element can remain stationary as, and second Support member includes horizontally disposed first supporting surface and the second supporting surface perpendicular to the first supporting surface, and such setting can ensure When glass slide is in heeling condition, it will not be slid towards inclined direction.Above setting make user using this incubation box into When row is incubated and rinses, it may not need one by one to take out glass slide and put back to again, only need to control the lever being piercing in outside box body, The activity of multiple glass slides with row's setting can be controlled simultaneously, it is more convenient.
Description of the drawings
Fig. 1 is the structure chart for the incubation box that one embodiment of the invention is related to;
Fig. 2 is the structure chart for the incubation box that another embodiment of the present invention is related to.
Reference sign:
1, box body;
2, the first support element;
20, lever;
21, handle;
3, the second support element;
30, liquid-leaking nozzle;
5, transmission belt;
6, box cover;
60, handle;
7, jet pipe;
70, hole for water spraying;
8, hose;
9, rubber bottle.
Specific implementation mode
For the technology contents of technical solution, construction feature, the objects and the effects are described in detail, below in conjunction with specific reality It applies example and attached drawing is coordinated to be explained in detail.
It is the experiment equipment that slice is incubated in Pathological experiment to be incubated box, is provided in existing incubation box and is used to support load glass Multiple rows of support column perpendicular to incubation cassette bottom face of piece, experimenter need to be positioned over the glass slide after dyeing to be incubated in box to cover It is incubated a period of time after box cover, needs to be dyed using a variety of different reagents and is incubated again, under use before a kind of reagent It needs to rinse well former reagent, therefore, whole process is to dye, be incubated, rinsing, draining excessive buffer, then contaminating Color is incubated, rinses again, draining excessive buffer again again, and draining the process of excessive buffer, need will glass slide one by one Drying is taken out from being incubated in box, places into and is incubated in box after drying, such operation is excessively cumbersome.
It please refers to Fig.1 to Fig. 2, for this purpose, the present invention provides a kind of incubation box, including box body 1 and box cover 6, the box body 1 Vessel is provided to be incubated, the box cover 6 can prevent dust from entering in box body, ensure that the space being incubated is relatively independent.Institute Square, cuboid, cylinder or other polyhedrons can be arranged to by stating box body, and box body is preferably arranged to cuboid, this The setting of sample is not only convenient for producing, and since glass slide is rectangle, such setting can also save more material to place The space in box body is farthest utilized in more glass slides.Similarly, the box cover can be arranged to and box body phase Square, cuboid, cylinder or other polyhedrons matched, since box body is preferably disposed to cuboid, the box cover It is preferably set to the cuboid to match with box body.
In a further embodiment, for the ease of lifting down box cover from box body, handle is provided on the box cover 60, the handle can be Pear-Shaped pillar, or the handle of arc.Handle can be fixed by screws on box cover, It can also be fixed by glass cement.
In a further embodiment, it is incubated slice in box since during the experiment, experimenter needs momentarily to observe Dyeing progress, in order not to interfere the incubation of slice, the box body to need the Transparent color for being arranged for ease of observation, therefore the box The material of body can be transparent glass or plastics, in order to cost-effective and take light and handy, it is preferred to use transparent modeling This box body is made in material, in order to which beautiful and adaptation, the box cover can also be made with box body using transparent plastics.Due to After repeatedly dyeing and rinse, the bottom surface of box body fills a large amount of liquid, and entire box body is with regard to pen tip weight when taking, if using Heavier glass is made, and use is more inconvenient, but not easy to wear.
Support component due to being used to support glass slide is horizontally set in box body, i.e., is arranged in parallel with the width of box body, is Multiple glass slides can be placed simultaneously with row, in a further embodiment, the width of the box body is 5cm-30cm, in this way Setting will not make excessive very much not easy to remove of box body, while can set glass slide as much as possible in same discharge again.
In the particular embodiment, the support component of at least one set of support glass slide, a branch are provided in the box body Support component is used to support the glass slide of a row.The support component is located at the top of box bottom, and such setting can prevent Slice in support component will not be flooded by the PBS buffers positioned at box body bottom, can ensure to drain excessive buffering Slice after agent it is clean, preferably the height of support component is set at 2/3rds height of box body, it is such to set Setting can be as far away from the PBS buffer positioned at box body bottom, and can prevent slice from being pushed down by box cover.
In the particular embodiment, the support component includes adjacent and horizontally disposed first support element 2 and second Support member 3, relatively independent between the first support element 2 and the second support element 3, the both ends of the glass slide are set up in first respectively In support member and the second support element.Wherein, first support element is the supporting rod that section is cam shape, second support element Height residing for first supporting surface, with the convex surface of supporting rod not upward when supporting surface face upward residing height one It causes, when such setting is so that the convex surface of supporting surface is not in direction upward, when glass slide is positioned in support component, carries glass Piece is in horizontal state.
Excessive buffer is drained after being rinsed for the ease of glass slide, in the particular embodiment, the one of the supporting rod End face is fixedly installed lever 20, and the lever 20 is movably arranged in outside box body, the other end of supporting rod and box The one side wall of body is flexibly connected;The supporting rod can be axially rotationally set in box body between two side.Such setting makes It obtains when needing to drain the glass slide after rinsing, user need to only control lever, control supporting rod axial-rotation so that supporting rod Convex surface be in state upward, glass slide at this time is in heeling condition, and the upper buffer of glass slide can be with inclination Direction flow to the bottom of box body, after carrying and draining excessive buffer, then controls lever rotation, supporting rod is driven to do axial fortune Dynamic, until the convex surface of supporting rod is not upward, glass slide returns to horizontality, and user can carry out slice dyeing next time. Such setting so that user to the dyeing of glass slide, rinse and drain excessive buffer and become convenient and efficient.
In a further embodiment, the lever can be integrally formed with supporting rod, can also be lever and This supporting rod is respectively independent, and by being composed, if lever is bolt or threaded rod, the end face of supporting rod offers Threaded hole compatible with the bolt or threaded rod, bolt or threaded rod are threaded onto the lever that supporting rod is formed in threaded hole.For Easy to produce and assembling be the mode assembled preferably by lever and supporting rod.
The mode of the movable connection of first support element and box body can there are many, it is described in a certain embodiment Lever is threaded rod, and the both ends of the surface of the supporting rod are respectively arranged with threaded hole, and the two sides of the box body just open up over the ground Two circular cavities are rotary with nut on threaded rod, and the threaded rod for being rotary with nut passes through a hole on box body, then screws in the one of supporting rod In the threaded hole of end face, such setting allow lever to drive supporting rod relative to box body rotation, and lever again will not be from Hole is deviate from.One bolt passes through another hole on box body, then screws in the threaded hole of other end of supporting rod, but is not necessarily to It screws, ensures that supporting rod will not fall down, and can be axially moved.
In the particular embodiment, the both ends of the surface of second support element 3 are fixedly connected with two madial walls of box body respectively, Second support element includes the first supporting surface and the second supporting surface, and the first supporting surface is in level, and second supporting surface is vertically It is connect with the first supporting surface, such setting carries when can prevent user from changing the angle of inclination of glass slide by controlling lever Slide slides.In order to prevent glass slide slide, the second supporting surface and the first supporting surface simply by the presence of angle, it is preferred that Second supporting surface is arranged perpendicular to the first supporting surface, i.e. the section of the second support element is L-shaped, can prevent glass slide from existing in this way When being back to horizontality from heeling condition by the second supporting surface clamp and can not return, can make glass slide successfully from inclination State is back to horizontality.
Since control lever drives supporting rod rotation, after driving glass slide to change angle of inclination, the liquid on glass slide Body is to flow to the second support element, then the bottom of box body is flowed upwardly to from the second support element, and excessive buffer is trapped in order to prevent On second support element, in a further embodiment, second support element offers liquid-leaking nozzle 30, i.e. the first supporting surface and Two supporting surfaces are wire side, and such setting is so that buffer either flows to the second supporting surface, or supports surface current from second To the first supporting surface, the bottom of box body can be flowed to by liquid-leaking nozzle, accelerates the speed for draining excessive buffer, dropped The low retention degree of excessive buffer.
The length of universal glass slide is 7.5cm, in order to support glass slide, and prevents glass slide from falling off, the first support The spacing of part and the second support element cannot be wide, can not be narrow, therefore, in a further embodiment, first support The spacing of part and the second support element is 5cm-6cm, since the first support element and the second support element itself also have certain width, When the spacing of the first support element and the second support element is 5cm, glass slide will not be fallen because of unbalance, when the first support element and When the spacing of second support element is 6cm, glass slide is also at not exclusively hanging above the first support element and the second support element And it falls.
In the above-described embodiment, between each support component independently of each other, the load glass in each support component is controlled The angle of inclination of piece needs the lever for controlling support component respectively, movable while in order to realize multiple support components, into In the embodiment of one step, the support component is provided at least two, the lever of two and more than two first support elements Between be sequentially connected by transmission belt 5, one of lever 20 is connected with handle 21.Specifically, conveyer belt 5 is to be end-to-end Belt, the lever be cylindrical bar.When only there are two when support component, belt is placed on outside two levers, and by two Lever support is straight, when a lever activity, belt activity, movable belt is driven to pass through the friction between belt and lever Power drives another lever to move synchronously, and realizes that two support components move synchronously, and realizes the synchronous fortune of two rows of glass slides It is dynamic.When support component is more than two, belt is placed on outside levers, and straight by two levers supports of head and the tail, positioned at from beginning to end two Lever between a lever is close to belt, can rubbing by belt and lever when one of lever activity Wiping power drives other levers to move synchronously, and to realize moving synchronously for multiple support components, finally realizes plurality of rows of glass slide It moves synchronously, it is more convenient in this way, reduce more operating procedures.
When rinsing slice with PBS, previous mode is in turn toward the sprinkling another whipping of PBS buffer solution on glass slide Slice removal excess PBS, such mode is very complicated, and therefore, in a further embodiment, this incubation box further includes jet pipe 7, one end of hose 8 and rubber bottle 9, the jet pipe is equipped with nozzle, and the tube wall of pipe spray offers hole for water spraying;The jet pipe is set to In box cover and nozzle is piercing in the cover rim of box cover, and hole for water spraying is oppositely arranged with support component;One end of hose is placed at nozzle, separately One end is placed on the bottle mouth position of rubber bottle.When needing to rinse slice, it is only necessary to squeeze rubber bottle, the buffer in rubber bottle It to be extruded into hose, then passes in jet pipe, is sprayed on slice finally by spray orifice.Such setting is when rinsing slice It is more convenient, it is not necessarily to multi-pass operation.
It should be noted that although the various embodiments described above have been described herein, it is not intended to limit The scope of patent protection of the present invention.Therefore, based on the present invention innovative idea, to embodiment described herein carry out change and repair Change, or using equivalent structure or equivalent flow shift made by description of the invention and accompanying drawing content, it directly or indirectly will be with Upper technical solution is used in other related technical areas, is included within the scope of patent protection of the present invention.

Claims (9)

1. a kind of incubation box, which is characterized in that including box body and box cover;At least one set of support glass slide is provided in the box body Support component, the support component is located at the top of box bottom, and the support component includes adjacent and horizontally disposed One support element and the second support element;
First support element is the supporting rod that section is cam shape, and the end face of the supporting rod is fixedly installed lever, The lever is movably arranged in outside box body, and the other end of supporting rod is flexibly connected with the one side wall of box body;The branch Strut can be axially rotationally set in box body between two side;
The both ends of the surface of second support element are fixedly connected with two madial walls of box body respectively, and the second support element includes the first support Face and the second supporting surface, second supporting surface are vertically connect with the first supporting surface.
2. incubation box according to claim 1, which is characterized in that the support component is provided at least two, first It is sequentially connected by transmission belt between the lever of support member, a lever is connected with handle.
3. incubation box according to claim 1, which is characterized in that further include bolt, the end face of the lever is provided with The middle part of threaded rod, the first support element both ends of the surface offers bolt hole respectively, and the two side of the box body opens up hole respectively, Bolt and threaded rod are through at the hole of box body two side respectively, and are threaded onto the threaded hole of the first support element both ends of the surface respectively.
4. incubation box according to claim 1, which is characterized in that second support element offers liquid-leaking nozzle.
5. incubation box according to claim 1, which is characterized in that be provided with handle on the box cover.
6. incubation box according to claim 1, which is characterized in that further include jet pipe, hose and rubber bottle, the jet pipe One end is equipped with nozzle, and the tube wall of pipe spray offers hole for water spraying;The jet pipe is set in box cover, and hole for water spraying and support component phase To setting;The nozzle of jet pipe is pierced by outside box cover, and one end of hose is placed at nozzle, and the other end is placed on the bottle mouth position of rubber bottle.
7. incubation box according to claim 1, which is characterized in that the material of the box body is transparent glass or plastics.
8. incubation box according to claim 1, which is characterized in that the width of the box body is 5cm-30cm.
9. incubation box according to claim 1, which is characterized in that the spacing of first support element and the second support element is 5cm-6cm。
CN201810375295.9A 2018-04-24 2018-04-24 A kind of incubation box Pending CN108362869A (en)

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Application Number Priority Date Filing Date Title
CN201810375295.9A CN108362869A (en) 2018-04-24 2018-04-24 A kind of incubation box

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Application Number Priority Date Filing Date Title
CN201810375295.9A CN108362869A (en) 2018-04-24 2018-04-24 A kind of incubation box

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CN108362869A true CN108362869A (en) 2018-08-03

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Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2189743Y (en) * 1994-02-07 1995-02-15 刘东风 Multifunctional immunohistochemical incubation dyeing device
CN1574142A (en) * 2003-05-30 2005-02-02 三美电机株式会社 Key switching device
CN202066732U (en) * 2011-06-03 2011-12-07 天津市宝坻区人民医院 Constant-temperature incubation device for immunohistochemistry
CN204185583U (en) * 2014-09-15 2015-03-04 湖北华昌达智能装备股份有限公司 A kind of draining roller bed and Workpiece coating system
CN204804711U (en) * 2015-06-24 2015-11-25 中国石油天然气股份有限公司 Drilling tool drag reducer
CN105571925A (en) * 2016-03-09 2016-05-11 福州迈新生物技术开发有限公司 Dyeing module applied to biological samples on glass slides and dyeing method thereof
CN206102516U (en) * 2016-06-02 2017-04-19 张今 A drain device for family
CN106769351A (en) * 2015-11-23 2017-05-31 内蒙古蒙牛乳业(集团)股份有限公司 Microbial staining frame
CN206799586U (en) * 2017-05-27 2017-12-26 盐城卫生职业技术学院 A kind of culture dish draining rack
CN208076531U (en) * 2018-04-24 2018-11-09 福州迈新生物技术开发有限公司 A kind of incubation box

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2189743Y (en) * 1994-02-07 1995-02-15 刘东风 Multifunctional immunohistochemical incubation dyeing device
CN1574142A (en) * 2003-05-30 2005-02-02 三美电机株式会社 Key switching device
CN202066732U (en) * 2011-06-03 2011-12-07 天津市宝坻区人民医院 Constant-temperature incubation device for immunohistochemistry
CN204185583U (en) * 2014-09-15 2015-03-04 湖北华昌达智能装备股份有限公司 A kind of draining roller bed and Workpiece coating system
CN204804711U (en) * 2015-06-24 2015-11-25 中国石油天然气股份有限公司 Drilling tool drag reducer
CN106769351A (en) * 2015-11-23 2017-05-31 内蒙古蒙牛乳业(集团)股份有限公司 Microbial staining frame
CN105571925A (en) * 2016-03-09 2016-05-11 福州迈新生物技术开发有限公司 Dyeing module applied to biological samples on glass slides and dyeing method thereof
CN206102516U (en) * 2016-06-02 2017-04-19 张今 A drain device for family
CN206799586U (en) * 2017-05-27 2017-12-26 盐城卫生职业技术学院 A kind of culture dish draining rack
CN208076531U (en) * 2018-04-24 2018-11-09 福州迈新生物技术开发有限公司 A kind of incubation box

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