CN108348435A - Containing as active ingredient Cyanidin -3-O- glucosides and the anti-aging of natto gum use Dermatologic preparation composition - Google Patents
Containing as active ingredient Cyanidin -3-O- glucosides and the anti-aging of natto gum use Dermatologic preparation composition Download PDFInfo
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- CN108348435A CN108348435A CN201680062930.7A CN201680062930A CN108348435A CN 108348435 A CN108348435 A CN 108348435A CN 201680062930 A CN201680062930 A CN 201680062930A CN 108348435 A CN108348435 A CN 108348435A
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- cyanidin
- glucosides
- natto gum
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- dermatologic preparation
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
- A61K31/7048—Compounds having saccharide radicals and heterocyclic rings having oxygen as a ring hetero atom, e.g. leucoglucosan, hesperidin, erythromycin, nystatin, digitoxin or digoxin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
Abstract
This is bright to be related to containing 3 O glucosides of Cyanidin and natto gum as the anti-aging with Dermatologic preparation composition of active ingredient, relates in particular to play the Dermatologic preparation composition of the inhibition to collagen catabolic enzyme by the mixture containing 3 O glucosides of Cyanidin and natto gum.When using Dermatologic preparation composition of the present invention, with effectively eliminating to excellent antioxidant effects such as the harmful free radicals of skin, expression and its activity for inhibiting collagen catabolic enzyme improve the excellent antiageing effects such as wrinkle to reach enhancing skin elasticity.
Description
Technical field
The present invention relates to contain Cyanidin -3-O- glucosides and natto gum to use skin as the anti-aging of active ingredient
Preparation composition for external use relates in particular to play by the mixture containing Cyanidin -3-O- glucosides and natto gum
To the Dermatologic preparation composition of the inhibition of collagen catabolic enzyme.
Background technology
First protecting film of the skin as human body, variation and ultraviolet light, public hazards substance from temperature and humidity etc. are external
All organs in the stimulation of environment in protective, important function is also functioned in the maintenance of the homeostasises such as body heat regulation.But
The normal function that skin can be reduced by excessive physical, chemical irritation and pressure, malnutrition etc. from outside, promotes bullet
Property loss, keratinization, wrinkle generate etc. skin ageing phenomenons generation.
These phenomenons and more healthy and flexible skin is kept in order to prevent, has been devoted to prepare containing from each
The cosmetics of the bioactive substance obtained in kind of plant, microorganism etc., intrinsic function for keeping skin simultaneously keep skin thin
Cytoactive, to effectively inhibit skin aging.But there is most of efficiency is insufficient or lure for previous cosmetic material
Send out the various problems such as skin side-effects.
Cyanidin -3-O- glucosides (cyanidin-3-O-glucoside) are one kind of anthocyanidin, also referred to as chrysanthemum color
Plain (chrysanthemin), is the knot formed with glucoside bond combination Cyanidin on the third carbon atom of glucose
Structure.Pigment containing a large amount of display color in known berry fruits or cereal etc., this kind of pigment have antioxidant effect,
Nearest research report points out, Cyanidin -3-O- glucosides can effectively pre- anti-cancer or heart disease (Cooke et al.,
International journal of cancer 119.9(2006):2213-2220;Serraino et al.,Life
sciences 73.9(2003):1097-1114).But there are no reports to point out so far, Cyanidin -3-O- grapes
Whether glucosides can inhibit collagen catabolic enzyme, to the antiageing effect for keeping skin elasticity and improvement wrinkle.
On the one hand, natto gum (natto gum) is in soybean (soybean), using being called hay bacillus (Bacillus
Subtilis high viscosity substance obtained from gram-positive bacteria) is fermented.It is developed recently containing playing thickener
And the cosmetics of the natto gum with strength moistening effect.But it is identical as Cyanidin -3-O- glucosides, so far
There are no reports to point out, whether natto gum can inhibit collagen catabolic enzyme, to holding skin elasticity and improvement wrinkle
The antiageing effect of line, it is not indicated that whether natto gum can improve the antioxidant effect of Cyanidin -3-O- glucosides
Or antiageing effect.
The present inventor is deeply ground to find skin preventing aging effect from reaching the natural materials of maximization
Study carefully, as a result, it has been found that, the Dermatologic preparation composition of the mixture containing Cyanidin -3-O- glucosides and natto gum can press down
Free radical processed generates and the expression of effective inhibition collagen catabolic enzyme, to reach very excellent antioxidant effect and anti-ageing
Change effect, thus completes the present invention.
【Existing technical literature】
【Non-patent literature】
1、Cooke et al.,International journal of cancer 119.9(2006):2213-2220
2、Serraino et al.,Life sciences 73.9(2003):1097-1114
Invention content
The object of the present invention is to provide contain Cyanidin -3-O- glucosides and natto gum as the anti-ageing of active ingredient
Change Dermatologic preparation composition, the composition is by inhibiting the generation of free radical (free radical) in skin, inhibiting glue
The expression of former protease, to show antiageing effect.
To achieve the goals above, present invention offer contains Cyanidin -3-O- glucosides and natto gum as effective
The anti-aging of ingredient uses Dermatologic preparation composition.
In addition, the present invention provides containing Cyanidin -3-O- glucosides (cyanidin-3-O-glucoside) and receives
Skin elasticity improvement Dermatologic preparation composition of the bean gum as active ingredient.
In addition, the present invention provides containing Cyanidin -3-O- glucosides (cyanidin-3-O-glucoside) and receives
Wrinkle of skin improvement Dermatologic preparation composition of the bean gum as active ingredient.
In addition, the present invention provides Cyanidin -3-O- glucosides and natto gum as skin elasticity improver in skin
Purposes in the preparation of preparation composition for external use (specifically, cosmetic composition or pharmaceutical compositions).
In addition, the present invention provides Cyanidin -3-O- glucosides and natto gum as wrinkle of skin improver in skin
Purposes in the preparation of preparation composition for external use (specifically, cosmetic composition or pharmaceutical compositions).
When using Dermatologic preparation composition of the invention, effectively eliminate excellent to the harmful free radical of skin etc.
Antioxidant effect, by inhibiting expression and the activity of collagen catabolic enzyme (collagenase), to reach enhancing skin bullet
Property and improve the excellent antiageing effect such as wrinkle.
Specific implementation mode
Other than otherwise defining, all technologies and scientific words that are used in this specification refer to having and this
Skilled expert in technical field that the present invention belongs to it can be generally understood that the identical meaning of meaning.In general, in this specification
The nomenclature used is that the art is known and common nomenclature.
In an embodiment of the present invention, it is measured after Cyanidin -3-O- glucosides and natto gum being mixed with equal amount
To the inhibition of DPPH oxidations, as a result, it can be seen that, be used alone Cyanidin -3-O- glucosides or natto gum or
Person is by other pigments in anthocyanidin-delphinium anthocyanidin -3-O- glucosides (delphinidin-3-O-glucoside) and receives
It is compared when bean gum is used in mixed way, shows more excellent antioxidant effect.In addition, by Cyanidin -3-O- glucosides and
Natto gum with equal amount or close to equal amount mix after, measure its inhibition to collagen catabolic enzyme, by result it is found that
With Cyanidin -3-O- glucosides or natto gum is used alone or by delphinium anthocyanidin -3-O- glucosides and natto
It is compared when glue is used in mixed way, shows more excellent antiageing effect.
Therefore, one aspect of the present invention is related to containing Cyanidin -3-O- glucosides (cyanidin-3-O-
) and Dermatologic preparation composition of the natto gum as active ingredient glucoside.
In addition, another aspect of the present invention is related to containing Cyanidin -3-O- glucosides (cyanidin-3-O-
Glucoside) and natto gum as active ingredient skin elasticity improve with or wrinkle of skin improvement combined with skin preparations for extenal use
Object.
Cyanidin -3-O- the glucosides of the present invention have structure shown in following chemical formula 1, can utilize this technology
The conventional method in field, directly extraction uses from the plant containing a large amount of Cyanidin -3-O- glucosides, can also buy
Cyanidin -3-O- the glucosides refined after extraction are used.
【Chemical formula 1】
In the present invention, which is characterized in that above-mentioned natto gum be the hay bacillus (Bacillus subtilis) that will be activated or
Bacillus licheniformis (Bacillus licheniformis) is seeded in soybean and is fermented and generated.Natto gum is will be withered
Straw bacterium or bacillus licheniformis are inoculated in soybean, ferment under assigned temperature, the stickum of soybean Surface Creation, will
After its refined, crystallization, in the composition with powder morphology for the present invention.Type for the fermentation bean or pea for generating natto gum,
Most preferably soybean, but not limited to this.
When using the composition for being mixed together natto gum and Cyanidin -3-O- glucosides, it may be speculated that natto
The negatively charged polyglutamic acid contained in glue can be with the interaction of positively charged Cyanidin -3-O- glucosides so that arrow
The stable structure of Che Jusu -3-O- glucosides, to further increase the anti-oxidant and anti-of Cyanidin -3-O- glucosides
Aging effect.
In the present invention, which is characterized in that contain the skin of Cyanidin -3-O- glucosides and natto gum as active ingredient
Collagen decomposition of the skin preparation composition for external use due to inhibiting collagen, reduction skin elasticity for decomposing skin histology
The expression of enzyme, therefore show antiageing effect, especially wrinkle of skin improvement and skin elasticity improvement.In addition,
In the present invention, " collagen catabolic enzyme " and " clostridiopetidase A (collagenase) " is used with identical meanings.
In addition, in the present invention, which is characterized in that the mixed weight of above-mentioned Cyanidin -3-O- glucosides and natto gum
Than being about 1:1~1:10, preferably about 1:1~1:9, more preferably about 1:1~1:7, further preferably about 1:1~1:5, more
Further preferably about 1:1~1:3, further preferably 3:2~2:3, further preferably 5:4~4:5, it is optimal
It is selected as 1:1.When being mixed with the weight ratio in above range, can be conducive to Cyanidin -3-O- glucosides and
The inhibition that expression of enzymes is decomposed to collagen that natto gum respectively has maximizes, and meets the stability of composition.
In addition, in the present invention, which is characterized in that relative to the total weight of composition, above-mentioned Cyanidin -3-O- glucose
The content of glycosides is 0.01~5 weight %;Relative to the total weight of composition, the content of above-mentioned natto gum is 0.01~5 weight %.
When the summation of above two active ingredient is less than 0.02 weight %, the inhibition that expression of enzymes is decomposed to collagen is micro-
It is weak;When more than 10 weight %, composition can have that viscosity is excessively high again.
When Dermatologic preparation composition dosage form of the present invention is turned to cosmetics form, skin softening can be melted into dosage form
Water, astringent lotion, nutrition toner, eye cream, nourishing cream, massage cream, cleansing cream, cleaning foam, clean water, loose powder, essence or
The forms such as facial mask, but its dosage form is not particularly limited in this.In addition, the composition of the present invention, which can contain, is commonly used in cosmeceutical
Or the auxiliary agent in Dermatology field, such as it is fatty material, organic solvent, solubilizer, thickener, gelling agent, softening agent, anti-oxidant
Agent, suspending agent, stabilizer, foaming agent (foaming agent), aromatic, surfactant, water, ionic or nonionic
Emulsifier, filler, metal ion block agent, chelating agent, preservative, vitamin, retarding agent, wetting agent, essential oil, dyestuff, face
Material, hydrophily or lipophile activator, lipid vesicle or commonly used in any other ingredient in cosmetics.Above-mentioned auxiliary agent is to change
Cosmetic or the usual dosage in Dermatology field are added.The composition of the present invention, which can also contain, promotes skin absorption
Substance, to increase the effect for improving skin.
Dermatologic preparation composition of the present invention in addition to the foregoing, in the range for not influencing main effect
It is interior, it is preferable that the other compositions that can promote main effect can also be contained.Moreover, it relates to skin preparations for extenal use group
It closes object and further includes moisturizer, softening agent, ultra-violet absorber, preservative, fungicide, antioxidant, pH adjusting agent, organic
With inorganic pigment, fragrance, cool agent or antiperspirant etc..The combined amount of mentioned component is not influencing the objects and effects of the present invention
In range, those skilled in the art can be easy to carry out selection, relative to the total weight of composition, combined amount 0.01-5
Weight %, preferably 0.01-3 weight %.
The embodiment of invention
The specific part of the content of present invention is described in detail above, and those who have general knowledge should be understood that in this field
These particular techniques only belong to preferred embodiment, and it's not limited to that for the scope of the present invention.Therefore, of the invention essence
Range is defined according to appended appended claims and their equivalents.
【Reference example 1】
Cyanidin -3-O- glucosides (cyanidin-3-O- for the effect for testing the present composition
Glucoside sigma companies) are purchased from.
【Reference example 2】
Delphinium anthocyanidin -3-O- glucosides (delphinidin-3- for the effect for testing the present composition
O-glucoside sigma companies) are purchased from.
【Reference example 3】
The natto gum of effect for testing the present composition is prepared by following methods.
(1) heat sterilization process
By soybeans soaking 10~15 hours or so in water, after ionized water (first water) fully washing, in height
It presses in sterilizer (autoclave), 100~121 DEG C of range, heat sterilization about 2 hours.
(2) culture processes
Sterilized soybean is cooled to 40 DEG C, by the hay bacillus inoculation liquid of activation with the 5 of opposite soybean total weight~
After 10% inoculation, cultivated at 35~40 DEG C about 36 hours.By hay bacillus with 107~8The concentration of Cell/ml is inoculated in 8g/L's
In nutrient medium (Neutrient Brooth), inoculation liquid is formed.
(3) extraction and refining step
Ionized water is added in right amount in the soy broth for completing culture, is sufficiently stirred the sticky shape substance for making soybean surface
After being dissolved in ionized water, filtering obtains transparent extracting solution.
(4) Crystallization Procedure
It adds methanol, ethyl alcohol, acetone equal solvent in right amount in transparent extracting solution, reduces its solubility in water, obtain
To white depositions.Then, the solvent for including in obtained sediment is evaporated drying, obtains natto gum powder.
【The preparation of embodiment 1-3 and comparative example 1-3】
By the natto gum prepared in the Cyanidin -3-O- glucosides of above-mentioned reference example 1 and reference example 3 with following table 1
Shown in weight ratio mixed respectively, embodiment 1-3 is prepared with identical total weight.
In addition, the comparative example as above-described embodiment 1-3, by the delphinium anthocyanidin -3-O- glucose of above-mentioned reference example 2
The natto gum prepared in glycosides and reference example 3 is mixed respectively with weight ratio shown in following table 1, with identical as embodiment 1-3
Total weight prepare comparative example 1-3.
【Table 1】
(unit:Weight %)
【Test example 1】Antioxygenic property is tested
In order to observe Cyanidin -3-O- glucosides and natto gum mixture-embodiment 1-3 antioxidant effect,
By being measured and being compared based on caused absorbance change is restored by organic free radical-DPPH (1,1- diphenyl -2- picryls hydrazine)
Compared with exclusive use Cyanidin -3-O- glucosides, natto gum and delphinium anthocyanidin -3-O- glucosides is used alone
With the effect for inhibiting DPPH oxidations of the comparative example 1-3 of natto gum mixing.
By by the DPPH solution of 100 μM (in ethanol) of 190 μ l and Cyanidin -3-O- glucoside (reference examples
1), natto gum (reference example 3), embodiment 1-3, comparative example 1-3 and synthetized oxidation preventive agent-watermiscible vitamin E (Trolox)
After (positive controls) are prepared with identical 10000ppm concentration respectively, it is respectively 500ppm to be diluted to end reaction concentration,
After 250ppm, 125ppm, 62.5ppm, 31.25ppm, 15.63ppm, 10 μ l are separately added into, reaction solution is obtained, it is anti-at 37 DEG C
After answering 30 minutes, absorbance is measured at 540nm.Analysis result is shown in the following table 2, wherein IC50Indicate due to addition sample and
Sample concentration when absorbance being made to reduce by 50%.
【Table 2】
Substances | IC50(ppm) |
Watermiscible vitamin E (Trolox) (positive controls) | 43.3 |
Cyanidin -3-O- glucosides | 231.5 |
Delphinium anthocyanidin -3-O- glucosides | 287.5 |
Natto gum | There is no effect |
Embodiment 1 | 39.2 |
Embodiment 2 | 108.5 |
Embodiment 3 | 203.7 |
Comparative example 1 | 58.6 |
Comparative example 2 | 143.5 |
Comparative example 3 | 255.8 |
As shown in Table 2 above it is found that in the case where natto gum is used alone, almost without antioxidant effect.But
By Cyanidin -3-O- glucosides and natto gum with 1:In the embodiment 1 of 1 weight ratio mixing, IC50Value is given birth to water soluble vitamin
Plain E (Trolox) is similar, but compared with the case where Cyanidin -3-O- glucosides are used alone, but shows especially high
Antioxidant effect.In addition, by Cyanidin -3-O- glucosides and natto gum with 1:2 He of embodiment of 3 weight ratios mixing
With 1:In the embodiment 3 that 9 weight ratios are mixed, although not showing with 1:The such outstanding antioxygen of embodiment 1 of 1 mixing
Change effect, but compared with the case where Cyanidin -3-O- glucosides are used alone, effect is fine.In addition, embodiment 1-
3 with respectively by same amount of delphinium anthocyanidin -3-O- glucosides instead of Cyanidin -3-O- glucosides come with natto gum
Mixed comparative example 1-3 is compared, and antioxidant effect is more excellent.Hence, it can be determined that the composition of the present invention is with excellent
Antioxidant effect.
【Experimental example 2】Anti-aging property is tested
In order to observe Cyanidin -3-O- glucosides and natto gum mixture-embodiment 1-3 antiageing effect,
By ability that respective inhibition clostridiopetidase A (collagenase) generates with Cyanidin -3-O- glucosides, individually are used alone
The comparative example 1-3 and tocopherol or EGCG mixed using natto gum, delphinium anthocyanidin -3-O- glucosides and natto gum
It is compared, measures.Known, tocopherol and EGCG are with the object for regenerating the epidermal cell of skin, the performance for preventing aging
Matter.
Human fibroblasts (Cascade Biologics companies) (Portland, OR, U.S.A.) are added to be equipped with and are contained
96 holes of DMEM (Dulbecco's Modified Eagle's Media) culture medium of the fetal calf serum of 2.5 weight % are micro
In titer plate (96-well microtiter plate), per 5000, hole cell (well), culture to coverage rate reaches about
90%.Then, after being cultivated 24 hours in plasma-free DMEM medium, being dissolved in identical 50ppm concentration for 10 μ g is taken respectively
It is the Cyanidin -3-O- glucosides (reference example 1) of plasma-free DMEM medium, natto gum (reference example 3), embodiment 1-3, right
Ratio 1-3 is handled 24 hours, uses tocopherol and EGCG (Sigma Aldriches) processing 24 hours of 50 μM of concentration respectively
Afterwards, cell culture fluid is harvested.
Collagen in the cell culture fluid of harvest is measured using clostridiopetidase A measuring instrument (Amersham Pharmacia, USA)
The generation degree of enzyme.First, the cell culture fluid of harvest is added to 96 orifice plates for being uniformly applied with clostridiopetidase A primary antibody
In (96-well plate), and progress antigen-antibody reaction 3 hours in constant temperature bath (36 DEG C).
After 3 hours, to after addition is combined with the clostridiopetidase A secondary antibody of luminophore in 96 orifice plates (96-well plate), instead
It answers 15 minutes.After 15 minutes, the substance of inducing color development is added, at room temperature inducing color development 15 minutes, is terminated when 1M sulfuric acid is added
When reacting (colour developing), reaction solution is shown as yellow, and with the progress of reaction, the intensity of yellow changes.
The absorbance for 96 orifice plates (96-well plate) that yellow is presented is measured at 405nm with spectrophotometer, and is made
The synthesis degree of clostridiopetidase A is calculated with following mathematical expressions 1.At this point, by the cell culture fluid obtained without the composition processing
React absorbance as a control group.That is, the expression 100 of the clostridiopetidase A relative to untreated fish group, calculates with examination
The expression of clostridiopetidase A in the group of substance processing is tested, the results are shown in following Table 3.
【Table 3】
Substances | The expression (%) of clostridiopetidase A |
Non-process group | 100 |
Tocopherol | 73 |
EGCG | 59 |
Cyanidin -3-O- glucosides | 78 |
Delphinium anthocyanidin -3-O- glucosides | 84 |
Natto gum | 94 |
Embodiment 1 | 51 |
Embodiment 2 | 67 |
Embodiment 3 | 75 |
Comparative example 1 | 61 |
Comparative example 2 | 73 |
Comparative example 3 | 80 |
As can be seen from Table 3, in the case where natto gum is used alone, it is virtually impossible to inhibit the expression of clostridiopetidase A.But
It is, by Cyanidin -3-O- glucosides and natto gum with 1:The embodiment 1 of 1 weight ratio mixing and exclusive use Cyanidin-
The case where 3-O- glucosides, is compared, and in vitro (in vitro) can more efficiently inhibit the expression of clostridiopetidase A,
Inhibit the ability of collagenase expression even more preferable than known as the tocopherol of antioxidant and the ability of EGCG.In addition,
By Cyanidin -3-O- glucosides and natto gum with 1:3 weight ratios mixing embodiment 2 and with 1:The reality of 9 weight ratios mixing
It applies in example 3, although not showing with 1:1 mixing embodiment 1 in it is such inhibit collagenase expression effect, but with list
It solely uses and is compared the case where Cyanidin -3-O- glucosides, effect is more excellent.Moreover, embodiment 1-3 with respectively by identical
Delphinium anthocyanidin -3-O- the glucosides of amount carry out the comparative example mixed with natto gum instead of Cyanidin -3-O- glucosides
1-3 is compared, and inhibits the effect of collagenase expression more excellent.Hence, it can be determined that the composition of the present invention is by inhibiting collagen
Expression of enzymes is to excellent antiageing effect, particularly skin elasticity improvement and wrinkle of skin improvement.
The characteristic of the content of present invention as detailed above, those who have general knowledge should be understood that these tools in this field
Body technique is only preferred embodiment, and it's not limited to that for the scope of the present invention.Therefore, the range of essence of the invention is basis
Appended appended claims and their equivalents define.
In addition, the following detailed description of the dosage form example of the Dermatologic preparation composition comprising the present composition, but should
It is realized that it's not limited to that for the scope of the present invention.
【Dosage form example 1】Nutrition toner
According to the composition that following table 4 is recorded, by conventional method, nutrition toner (unit is prepared:Weight %).
【Table 4】
Ingredient | Content |
Purified Water | Surplus |
Glycerine | 8.0 |
Butanediol | 4.0 |
Hyaluronan extraction object | 5.0 |
Beta glucan | 7.0 |
Carbomer | 0.1 |
Cyanidin -3-O- glucosides and natto gum (embodiment 1) | 0.05 |
Caprylic/capric triglyceride | 8.0 |
Saualane | 5.0 |
Cetearyl glucoside | 1.5 |
Stearic acid Isosorbide Dinitrate | 0.4 |
Cetostearyl alcohol | 1.0 |
Triethanolamine | 0.1 |
【Dosage form example 2】Nourishing cream
The composition recorded according to following table 5 prepares nourishing cream (unit by conventional method:Weight %).
【Table 5】
Ingredient | Content |
Purified Water | Surplus |
Glycerine | 3.0 |
Butanediol | 3.0 |
Liquid Paraffin | 7.0 |
Beta glucan | 7.0 |
Carbomer | 0.1 |
Cyanidin -3-O- glucosides and natto gum (embodiment 1) | 3.0 |
Caprylic/capric triglyceride | 3.0 |
Saualane | 5.0 |
Cetearyl glucoside | 1.5 |
Stearic acid Isosorbide Dinitrate | 0.4 |
Polysorbate60 | 1.2 |
Triethanolamine | 0.1 |
【Dosage form example 3】Massage cream
The composition recorded according to following table 6 prepares massage cream (unit by conventional method:Weight %).
【Table 6】
Ingredient | Content |
Purified Water | Surplus |
Glycerine | 8.0 |
Butanediol | 4.0 |
Liquid Paraffin | 45.0 |
Beta glucan | 7.0 |
Carbomer | 0.1 |
Cyanidin -3-O- glucosides and natto gum (embodiment 1) | 1.0 |
Caprylic/capric triglyceride | 3.0 |
Beeswax | 4.0 |
Cetearyl glucoside | 1.5 |
Sorbitan sesquioleate | 0.9 |
Vaseline | 3.0 |
Paraffin | 1.5 |
【Dosage form example 4】Facial mask
The composition recorded according to following table 7 prepares facial mask (unit by conventional method:Weight %).
【Table 7】
Ingredient | Content |
Purified Water | Surplus |
Glycerine | 4.0 |
Polyvinyl alcohol | 15.0 |
Hyaluronan extraction object | 5.0 |
Beta glucan | 7.0 |
Allantoin | 0.1 |
Cyanidin -3-O- glucosides and natto gum (embodiment 1) | 0.5 |
Nonylbenzene ether | 0.4 |
Polysorbate60 | 1.2 |
Ethyl alcohol | 6.0 |
【Dosage form example 5】Ointment in skin preparations for extenal use
The composition recorded according to following table 8 prepares ointment (unit by conventional method:Weight %).
【Table 8】
Ingredient | Content |
Purified Water | Surplus |
Glycerine | 8.0 |
Butanediol | 4.0 |
Liquid Paraffin | 15.0 |
Beta glucan | 7.0 |
Carbomer | 0.1 |
Cyanidin -3-O- glucosides and natto gum (embodiment 1) | 1.0 |
Caprylic/capric triglyceride | 3.0 |
Saualane | 1.0 |
Cetearyl glucoside | 1.5 |
Stearic acid Isosorbide Dinitrate | 0.4 |
Cetostearyl alcohol | 1.0 |
Beeswax | 4.0 |
Claims (10)
1. a kind of anti-aging Dermatologic preparation composition, the anti-aging Dermatologic preparation composition contain Cyanidin -3-
O- glucosides (cyanidin-3-O-glucoside) and natto gum are as active ingredient.
2. a kind of skin elasticity improvement Dermatologic preparation composition, which contains
Cyanidin -3-O- glucosides (cyanidin-3-O-glucoside) and natto gum are as active ingredient.
3. a kind of wrinkle of skin improvement Dermatologic preparation composition, which contains
Cyanidin -3-O- glucosides (cyanidin-3-O-glucoside) and natto gum are as active ingredient.
4. according to the Dermatologic preparation composition described in any one of claim 1-3, which is characterized in that the natto gum is
The hay bacillus (Bacillus subtilis) of activation or bacillus licheniformis (Bacillus licheniformis) are inoculated with
It is fermented and is generated in soybean.
5. according to the Dermatologic preparation composition described in any one of claim 1-3, which is characterized in that the composition is logical
It crosses and the expression of collagen catabolic enzyme is inhibited to play anti-aging effect.
6. according to the Dermatologic preparation composition described in any one of claim 1-3, which is characterized in that the corn flower
The mixed weight of element -3-O- glucosides and natto gum ratio is 1:1~1:10.
7. according to the Dermatologic preparation composition described in any one of claim 1-3, which is characterized in that relative to composition
Total weight, the contents of the Cyanidin -3-O- glucosides is 0.01~5 weight %;Relative to the total weight of composition,
The content of the natto gum is 0.01~5 weight %.
8. according to the Dermatologic preparation composition described in any one of claim 1-3, which is characterized in that the external preparation for skin
The chemical conversion of agent combination dosage form is selected from by smoothing toner, astringent lotion, nutrition toner, eye cream, nourishing cream, massage cream, cleansing cream, cleaning
More than one form in the group that foam, clean water, loose powder, essence or facial mask form.
9. a kind of Cyanidin -3-O- glucosides and natto gum are as skin elasticity improver in Dermatologic preparation composition
Purposes in preparation.
10. a kind of Cyanidin -3-O- glucosides and natto gum are as wrinkle of skin improver in Dermatologic preparation composition
Preparation in purposes.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/KR2016/010848 WO2018062581A1 (en) | 2016-09-28 | 2016-09-28 | Composition of anti-aging external application skin preparation, containing cyanidin-3-o-glucoside and natto gum as active ingredients |
Publications (2)
Publication Number | Publication Date |
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CN108348435A true CN108348435A (en) | 2018-07-31 |
CN108348435B CN108348435B (en) | 2022-02-25 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN201680062930.7A Active CN108348435B (en) | 2016-09-28 | 2016-09-28 | Anti-aging skin external composition containing cyanidin-3-O-glucoside and natto gum as effective components |
Country Status (5)
Country | Link |
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CN (1) | CN108348435B (en) |
HK (1) | HK1254521A1 (en) |
MY (1) | MY196008A (en) |
SG (1) | SG11201805443QA (en) |
WO (1) | WO2018062581A1 (en) |
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Also Published As
Publication number | Publication date |
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CN108348435B (en) | 2022-02-25 |
WO2018062581A1 (en) | 2018-04-05 |
HK1254521A1 (en) | 2019-07-19 |
SG11201805443QA (en) | 2018-07-30 |
MY196008A (en) | 2023-03-06 |
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