CN108348435A - Containing as active ingredient Cyanidin -3-O- glucosides and the anti-aging of natto gum use Dermatologic preparation composition - Google Patents

Containing as active ingredient Cyanidin -3-O- glucosides and the anti-aging of natto gum use Dermatologic preparation composition Download PDF

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Publication number
CN108348435A
CN108348435A CN201680062930.7A CN201680062930A CN108348435A CN 108348435 A CN108348435 A CN 108348435A CN 201680062930 A CN201680062930 A CN 201680062930A CN 108348435 A CN108348435 A CN 108348435A
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China
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cyanidin
glucosides
natto gum
preparation composition
dermatologic preparation
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CN201680062930.7A
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CN108348435B (en
Inventor
姜永圭
金妙姸
崔向兑
朴浚星
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Amorepacific Corp
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Amorepacific Corp
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/70Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7042Compounds having saccharide radicals and heterocyclic rings
    • A61K31/7048Compounds having saccharide radicals and heterocyclic rings having oxygen as a ring hetero atom, e.g. leucoglucosan, hesperidin, erythromycin, nystatin, digitoxin or digoxin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/48Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/02Cosmetics or similar toiletry preparations characterised by special physical form
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/60Sugars; Derivatives thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/08Anti-ageing preparations

Abstract

This is bright to be related to containing 3 O glucosides of Cyanidin and natto gum as the anti-aging with Dermatologic preparation composition of active ingredient, relates in particular to play the Dermatologic preparation composition of the inhibition to collagen catabolic enzyme by the mixture containing 3 O glucosides of Cyanidin and natto gum.When using Dermatologic preparation composition of the present invention, with effectively eliminating to excellent antioxidant effects such as the harmful free radicals of skin, expression and its activity for inhibiting collagen catabolic enzyme improve the excellent antiageing effects such as wrinkle to reach enhancing skin elasticity.

Description

Containing as active ingredient Cyanidin -3-O- glucosides and natto gum it is anti- Aging Dermatologic preparation composition
Technical field
The present invention relates to contain Cyanidin -3-O- glucosides and natto gum to use skin as the anti-aging of active ingredient Preparation composition for external use relates in particular to play by the mixture containing Cyanidin -3-O- glucosides and natto gum To the Dermatologic preparation composition of the inhibition of collagen catabolic enzyme.
Background technology
First protecting film of the skin as human body, variation and ultraviolet light, public hazards substance from temperature and humidity etc. are external All organs in the stimulation of environment in protective, important function is also functioned in the maintenance of the homeostasises such as body heat regulation.But The normal function that skin can be reduced by excessive physical, chemical irritation and pressure, malnutrition etc. from outside, promotes bullet Property loss, keratinization, wrinkle generate etc. skin ageing phenomenons generation.
These phenomenons and more healthy and flexible skin is kept in order to prevent, has been devoted to prepare containing from each The cosmetics of the bioactive substance obtained in kind of plant, microorganism etc., intrinsic function for keeping skin simultaneously keep skin thin Cytoactive, to effectively inhibit skin aging.But there is most of efficiency is insufficient or lure for previous cosmetic material Send out the various problems such as skin side-effects.
Cyanidin -3-O- glucosides (cyanidin-3-O-glucoside) are one kind of anthocyanidin, also referred to as chrysanthemum color Plain (chrysanthemin), is the knot formed with glucoside bond combination Cyanidin on the third carbon atom of glucose Structure.Pigment containing a large amount of display color in known berry fruits or cereal etc., this kind of pigment have antioxidant effect, Nearest research report points out, Cyanidin -3-O- glucosides can effectively pre- anti-cancer or heart disease (Cooke et al., International journal of cancer 119.9(2006):2213-2220;Serraino et al.,Life sciences 73.9(2003):1097-1114).But there are no reports to point out so far, Cyanidin -3-O- grapes Whether glucosides can inhibit collagen catabolic enzyme, to the antiageing effect for keeping skin elasticity and improvement wrinkle.
On the one hand, natto gum (natto gum) is in soybean (soybean), using being called hay bacillus (Bacillus Subtilis high viscosity substance obtained from gram-positive bacteria) is fermented.It is developed recently containing playing thickener And the cosmetics of the natto gum with strength moistening effect.But it is identical as Cyanidin -3-O- glucosides, so far There are no reports to point out, whether natto gum can inhibit collagen catabolic enzyme, to holding skin elasticity and improvement wrinkle The antiageing effect of line, it is not indicated that whether natto gum can improve the antioxidant effect of Cyanidin -3-O- glucosides Or antiageing effect.
The present inventor is deeply ground to find skin preventing aging effect from reaching the natural materials of maximization Study carefully, as a result, it has been found that, the Dermatologic preparation composition of the mixture containing Cyanidin -3-O- glucosides and natto gum can press down Free radical processed generates and the expression of effective inhibition collagen catabolic enzyme, to reach very excellent antioxidant effect and anti-ageing Change effect, thus completes the present invention.
【Existing technical literature】
【Non-patent literature】
1、Cooke et al.,International journal of cancer 119.9(2006):2213-2220
2、Serraino et al.,Life sciences 73.9(2003):1097-1114
Invention content
The object of the present invention is to provide contain Cyanidin -3-O- glucosides and natto gum as the anti-ageing of active ingredient Change Dermatologic preparation composition, the composition is by inhibiting the generation of free radical (free radical) in skin, inhibiting glue The expression of former protease, to show antiageing effect.
To achieve the goals above, present invention offer contains Cyanidin -3-O- glucosides and natto gum as effective The anti-aging of ingredient uses Dermatologic preparation composition.
In addition, the present invention provides containing Cyanidin -3-O- glucosides (cyanidin-3-O-glucoside) and receives Skin elasticity improvement Dermatologic preparation composition of the bean gum as active ingredient.
In addition, the present invention provides containing Cyanidin -3-O- glucosides (cyanidin-3-O-glucoside) and receives Wrinkle of skin improvement Dermatologic preparation composition of the bean gum as active ingredient.
In addition, the present invention provides Cyanidin -3-O- glucosides and natto gum as skin elasticity improver in skin Purposes in the preparation of preparation composition for external use (specifically, cosmetic composition or pharmaceutical compositions).
In addition, the present invention provides Cyanidin -3-O- glucosides and natto gum as wrinkle of skin improver in skin Purposes in the preparation of preparation composition for external use (specifically, cosmetic composition or pharmaceutical compositions).
When using Dermatologic preparation composition of the invention, effectively eliminate excellent to the harmful free radical of skin etc. Antioxidant effect, by inhibiting expression and the activity of collagen catabolic enzyme (collagenase), to reach enhancing skin bullet Property and improve the excellent antiageing effect such as wrinkle.
Specific implementation mode
Other than otherwise defining, all technologies and scientific words that are used in this specification refer to having and this Skilled expert in technical field that the present invention belongs to it can be generally understood that the identical meaning of meaning.In general, in this specification The nomenclature used is that the art is known and common nomenclature.
In an embodiment of the present invention, it is measured after Cyanidin -3-O- glucosides and natto gum being mixed with equal amount To the inhibition of DPPH oxidations, as a result, it can be seen that, be used alone Cyanidin -3-O- glucosides or natto gum or Person is by other pigments in anthocyanidin-delphinium anthocyanidin -3-O- glucosides (delphinidin-3-O-glucoside) and receives It is compared when bean gum is used in mixed way, shows more excellent antioxidant effect.In addition, by Cyanidin -3-O- glucosides and Natto gum with equal amount or close to equal amount mix after, measure its inhibition to collagen catabolic enzyme, by result it is found that With Cyanidin -3-O- glucosides or natto gum is used alone or by delphinium anthocyanidin -3-O- glucosides and natto It is compared when glue is used in mixed way, shows more excellent antiageing effect.
Therefore, one aspect of the present invention is related to containing Cyanidin -3-O- glucosides (cyanidin-3-O- ) and Dermatologic preparation composition of the natto gum as active ingredient glucoside.
In addition, another aspect of the present invention is related to containing Cyanidin -3-O- glucosides (cyanidin-3-O- Glucoside) and natto gum as active ingredient skin elasticity improve with or wrinkle of skin improvement combined with skin preparations for extenal use Object.
Cyanidin -3-O- the glucosides of the present invention have structure shown in following chemical formula 1, can utilize this technology The conventional method in field, directly extraction uses from the plant containing a large amount of Cyanidin -3-O- glucosides, can also buy Cyanidin -3-O- the glucosides refined after extraction are used.
【Chemical formula 1】
In the present invention, which is characterized in that above-mentioned natto gum be the hay bacillus (Bacillus subtilis) that will be activated or Bacillus licheniformis (Bacillus licheniformis) is seeded in soybean and is fermented and generated.Natto gum is will be withered Straw bacterium or bacillus licheniformis are inoculated in soybean, ferment under assigned temperature, the stickum of soybean Surface Creation, will After its refined, crystallization, in the composition with powder morphology for the present invention.Type for the fermentation bean or pea for generating natto gum, Most preferably soybean, but not limited to this.
When using the composition for being mixed together natto gum and Cyanidin -3-O- glucosides, it may be speculated that natto The negatively charged polyglutamic acid contained in glue can be with the interaction of positively charged Cyanidin -3-O- glucosides so that arrow The stable structure of Che Jusu -3-O- glucosides, to further increase the anti-oxidant and anti-of Cyanidin -3-O- glucosides Aging effect.
In the present invention, which is characterized in that contain the skin of Cyanidin -3-O- glucosides and natto gum as active ingredient Collagen decomposition of the skin preparation composition for external use due to inhibiting collagen, reduction skin elasticity for decomposing skin histology The expression of enzyme, therefore show antiageing effect, especially wrinkle of skin improvement and skin elasticity improvement.In addition, In the present invention, " collagen catabolic enzyme " and " clostridiopetidase A (collagenase) " is used with identical meanings.
In addition, in the present invention, which is characterized in that the mixed weight of above-mentioned Cyanidin -3-O- glucosides and natto gum Than being about 1:1~1:10, preferably about 1:1~1:9, more preferably about 1:1~1:7, further preferably about 1:1~1:5, more Further preferably about 1:1~1:3, further preferably 3:2~2:3, further preferably 5:4~4:5, it is optimal It is selected as 1:1.When being mixed with the weight ratio in above range, can be conducive to Cyanidin -3-O- glucosides and The inhibition that expression of enzymes is decomposed to collagen that natto gum respectively has maximizes, and meets the stability of composition.
In addition, in the present invention, which is characterized in that relative to the total weight of composition, above-mentioned Cyanidin -3-O- glucose The content of glycosides is 0.01~5 weight %;Relative to the total weight of composition, the content of above-mentioned natto gum is 0.01~5 weight %. When the summation of above two active ingredient is less than 0.02 weight %, the inhibition that expression of enzymes is decomposed to collagen is micro- It is weak;When more than 10 weight %, composition can have that viscosity is excessively high again.
When Dermatologic preparation composition dosage form of the present invention is turned to cosmetics form, skin softening can be melted into dosage form Water, astringent lotion, nutrition toner, eye cream, nourishing cream, massage cream, cleansing cream, cleaning foam, clean water, loose powder, essence or The forms such as facial mask, but its dosage form is not particularly limited in this.In addition, the composition of the present invention, which can contain, is commonly used in cosmeceutical Or the auxiliary agent in Dermatology field, such as it is fatty material, organic solvent, solubilizer, thickener, gelling agent, softening agent, anti-oxidant Agent, suspending agent, stabilizer, foaming agent (foaming agent), aromatic, surfactant, water, ionic or nonionic Emulsifier, filler, metal ion block agent, chelating agent, preservative, vitamin, retarding agent, wetting agent, essential oil, dyestuff, face Material, hydrophily or lipophile activator, lipid vesicle or commonly used in any other ingredient in cosmetics.Above-mentioned auxiliary agent is to change Cosmetic or the usual dosage in Dermatology field are added.The composition of the present invention, which can also contain, promotes skin absorption Substance, to increase the effect for improving skin.
Dermatologic preparation composition of the present invention in addition to the foregoing, in the range for not influencing main effect It is interior, it is preferable that the other compositions that can promote main effect can also be contained.Moreover, it relates to skin preparations for extenal use group It closes object and further includes moisturizer, softening agent, ultra-violet absorber, preservative, fungicide, antioxidant, pH adjusting agent, organic With inorganic pigment, fragrance, cool agent or antiperspirant etc..The combined amount of mentioned component is not influencing the objects and effects of the present invention In range, those skilled in the art can be easy to carry out selection, relative to the total weight of composition, combined amount 0.01-5 Weight %, preferably 0.01-3 weight %.
The embodiment of invention
The specific part of the content of present invention is described in detail above, and those who have general knowledge should be understood that in this field These particular techniques only belong to preferred embodiment, and it's not limited to that for the scope of the present invention.Therefore, of the invention essence Range is defined according to appended appended claims and their equivalents.
【Reference example 1】
Cyanidin -3-O- glucosides (cyanidin-3-O- for the effect for testing the present composition Glucoside sigma companies) are purchased from.
【Reference example 2】
Delphinium anthocyanidin -3-O- glucosides (delphinidin-3- for the effect for testing the present composition O-glucoside sigma companies) are purchased from.
【Reference example 3】
The natto gum of effect for testing the present composition is prepared by following methods.
(1) heat sterilization process
By soybeans soaking 10~15 hours or so in water, after ionized water (first water) fully washing, in height It presses in sterilizer (autoclave), 100~121 DEG C of range, heat sterilization about 2 hours.
(2) culture processes
Sterilized soybean is cooled to 40 DEG C, by the hay bacillus inoculation liquid of activation with the 5 of opposite soybean total weight~ After 10% inoculation, cultivated at 35~40 DEG C about 36 hours.By hay bacillus with 107~8The concentration of Cell/ml is inoculated in 8g/L's In nutrient medium (Neutrient Brooth), inoculation liquid is formed.
(3) extraction and refining step
Ionized water is added in right amount in the soy broth for completing culture, is sufficiently stirred the sticky shape substance for making soybean surface After being dissolved in ionized water, filtering obtains transparent extracting solution.
(4) Crystallization Procedure
It adds methanol, ethyl alcohol, acetone equal solvent in right amount in transparent extracting solution, reduces its solubility in water, obtain To white depositions.Then, the solvent for including in obtained sediment is evaporated drying, obtains natto gum powder.
【The preparation of embodiment 1-3 and comparative example 1-3】
By the natto gum prepared in the Cyanidin -3-O- glucosides of above-mentioned reference example 1 and reference example 3 with following table 1 Shown in weight ratio mixed respectively, embodiment 1-3 is prepared with identical total weight.
In addition, the comparative example as above-described embodiment 1-3, by the delphinium anthocyanidin -3-O- glucose of above-mentioned reference example 2 The natto gum prepared in glycosides and reference example 3 is mixed respectively with weight ratio shown in following table 1, with identical as embodiment 1-3 Total weight prepare comparative example 1-3.
【Table 1】
(unit:Weight %)
【Test example 1】Antioxygenic property is tested
In order to observe Cyanidin -3-O- glucosides and natto gum mixture-embodiment 1-3 antioxidant effect, By being measured and being compared based on caused absorbance change is restored by organic free radical-DPPH (1,1- diphenyl -2- picryls hydrazine) Compared with exclusive use Cyanidin -3-O- glucosides, natto gum and delphinium anthocyanidin -3-O- glucosides is used alone With the effect for inhibiting DPPH oxidations of the comparative example 1-3 of natto gum mixing.
By by the DPPH solution of 100 μM (in ethanol) of 190 μ l and Cyanidin -3-O- glucoside (reference examples 1), natto gum (reference example 3), embodiment 1-3, comparative example 1-3 and synthetized oxidation preventive agent-watermiscible vitamin E (Trolox) After (positive controls) are prepared with identical 10000ppm concentration respectively, it is respectively 500ppm to be diluted to end reaction concentration, After 250ppm, 125ppm, 62.5ppm, 31.25ppm, 15.63ppm, 10 μ l are separately added into, reaction solution is obtained, it is anti-at 37 DEG C After answering 30 minutes, absorbance is measured at 540nm.Analysis result is shown in the following table 2, wherein IC50Indicate due to addition sample and Sample concentration when absorbance being made to reduce by 50%.
【Table 2】
Substances IC50(ppm)
Watermiscible vitamin E (Trolox) (positive controls) 43.3
Cyanidin -3-O- glucosides 231.5
Delphinium anthocyanidin -3-O- glucosides 287.5
Natto gum There is no effect
Embodiment 1 39.2
Embodiment 2 108.5
Embodiment 3 203.7
Comparative example 1 58.6
Comparative example 2 143.5
Comparative example 3 255.8
As shown in Table 2 above it is found that in the case where natto gum is used alone, almost without antioxidant effect.But By Cyanidin -3-O- glucosides and natto gum with 1:In the embodiment 1 of 1 weight ratio mixing, IC50Value is given birth to water soluble vitamin Plain E (Trolox) is similar, but compared with the case where Cyanidin -3-O- glucosides are used alone, but shows especially high Antioxidant effect.In addition, by Cyanidin -3-O- glucosides and natto gum with 1:2 He of embodiment of 3 weight ratios mixing With 1:In the embodiment 3 that 9 weight ratios are mixed, although not showing with 1:The such outstanding antioxygen of embodiment 1 of 1 mixing Change effect, but compared with the case where Cyanidin -3-O- glucosides are used alone, effect is fine.In addition, embodiment 1- 3 with respectively by same amount of delphinium anthocyanidin -3-O- glucosides instead of Cyanidin -3-O- glucosides come with natto gum Mixed comparative example 1-3 is compared, and antioxidant effect is more excellent.Hence, it can be determined that the composition of the present invention is with excellent Antioxidant effect.
【Experimental example 2】Anti-aging property is tested
In order to observe Cyanidin -3-O- glucosides and natto gum mixture-embodiment 1-3 antiageing effect, By ability that respective inhibition clostridiopetidase A (collagenase) generates with Cyanidin -3-O- glucosides, individually are used alone The comparative example 1-3 and tocopherol or EGCG mixed using natto gum, delphinium anthocyanidin -3-O- glucosides and natto gum It is compared, measures.Known, tocopherol and EGCG are with the object for regenerating the epidermal cell of skin, the performance for preventing aging Matter.
Human fibroblasts (Cascade Biologics companies) (Portland, OR, U.S.A.) are added to be equipped with and are contained 96 holes of DMEM (Dulbecco's Modified Eagle's Media) culture medium of the fetal calf serum of 2.5 weight % are micro In titer plate (96-well microtiter plate), per 5000, hole cell (well), culture to coverage rate reaches about 90%.Then, after being cultivated 24 hours in plasma-free DMEM medium, being dissolved in identical 50ppm concentration for 10 μ g is taken respectively It is the Cyanidin -3-O- glucosides (reference example 1) of plasma-free DMEM medium, natto gum (reference example 3), embodiment 1-3, right Ratio 1-3 is handled 24 hours, uses tocopherol and EGCG (Sigma Aldriches) processing 24 hours of 50 μM of concentration respectively Afterwards, cell culture fluid is harvested.
Collagen in the cell culture fluid of harvest is measured using clostridiopetidase A measuring instrument (Amersham Pharmacia, USA) The generation degree of enzyme.First, the cell culture fluid of harvest is added to 96 orifice plates for being uniformly applied with clostridiopetidase A primary antibody In (96-well plate), and progress antigen-antibody reaction 3 hours in constant temperature bath (36 DEG C).
After 3 hours, to after addition is combined with the clostridiopetidase A secondary antibody of luminophore in 96 orifice plates (96-well plate), instead It answers 15 minutes.After 15 minutes, the substance of inducing color development is added, at room temperature inducing color development 15 minutes, is terminated when 1M sulfuric acid is added When reacting (colour developing), reaction solution is shown as yellow, and with the progress of reaction, the intensity of yellow changes.
The absorbance for 96 orifice plates (96-well plate) that yellow is presented is measured at 405nm with spectrophotometer, and is made The synthesis degree of clostridiopetidase A is calculated with following mathematical expressions 1.At this point, by the cell culture fluid obtained without the composition processing React absorbance as a control group.That is, the expression 100 of the clostridiopetidase A relative to untreated fish group, calculates with examination The expression of clostridiopetidase A in the group of substance processing is tested, the results are shown in following Table 3.
【Table 3】
Substances The expression (%) of clostridiopetidase A
Non-process group 100
Tocopherol 73
EGCG 59
Cyanidin -3-O- glucosides 78
Delphinium anthocyanidin -3-O- glucosides 84
Natto gum 94
Embodiment 1 51
Embodiment 2 67
Embodiment 3 75
Comparative example 1 61
Comparative example 2 73
Comparative example 3 80
As can be seen from Table 3, in the case where natto gum is used alone, it is virtually impossible to inhibit the expression of clostridiopetidase A.But It is, by Cyanidin -3-O- glucosides and natto gum with 1:The embodiment 1 of 1 weight ratio mixing and exclusive use Cyanidin- The case where 3-O- glucosides, is compared, and in vitro (in vitro) can more efficiently inhibit the expression of clostridiopetidase A, Inhibit the ability of collagenase expression even more preferable than known as the tocopherol of antioxidant and the ability of EGCG.In addition, By Cyanidin -3-O- glucosides and natto gum with 1:3 weight ratios mixing embodiment 2 and with 1:The reality of 9 weight ratios mixing It applies in example 3, although not showing with 1:1 mixing embodiment 1 in it is such inhibit collagenase expression effect, but with list It solely uses and is compared the case where Cyanidin -3-O- glucosides, effect is more excellent.Moreover, embodiment 1-3 with respectively by identical Delphinium anthocyanidin -3-O- the glucosides of amount carry out the comparative example mixed with natto gum instead of Cyanidin -3-O- glucosides 1-3 is compared, and inhibits the effect of collagenase expression more excellent.Hence, it can be determined that the composition of the present invention is by inhibiting collagen Expression of enzymes is to excellent antiageing effect, particularly skin elasticity improvement and wrinkle of skin improvement.
The characteristic of the content of present invention as detailed above, those who have general knowledge should be understood that these tools in this field Body technique is only preferred embodiment, and it's not limited to that for the scope of the present invention.Therefore, the range of essence of the invention is basis Appended appended claims and their equivalents define.
In addition, the following detailed description of the dosage form example of the Dermatologic preparation composition comprising the present composition, but should It is realized that it's not limited to that for the scope of the present invention.
【Dosage form example 1】Nutrition toner
According to the composition that following table 4 is recorded, by conventional method, nutrition toner (unit is prepared:Weight %).
【Table 4】
Ingredient Content
Purified Water Surplus
Glycerine 8.0
Butanediol 4.0
Hyaluronan extraction object 5.0
Beta glucan 7.0
Carbomer 0.1
Cyanidin -3-O- glucosides and natto gum (embodiment 1) 0.05
Caprylic/capric triglyceride 8.0
Saualane 5.0
Cetearyl glucoside 1.5
Stearic acid Isosorbide Dinitrate 0.4
Cetostearyl alcohol 1.0
Triethanolamine 0.1
【Dosage form example 2】Nourishing cream
The composition recorded according to following table 5 prepares nourishing cream (unit by conventional method:Weight %).
【Table 5】
Ingredient Content
Purified Water Surplus
Glycerine 3.0
Butanediol 3.0
Liquid Paraffin 7.0
Beta glucan 7.0
Carbomer 0.1
Cyanidin -3-O- glucosides and natto gum (embodiment 1) 3.0
Caprylic/capric triglyceride 3.0
Saualane 5.0
Cetearyl glucoside 1.5
Stearic acid Isosorbide Dinitrate 0.4
Polysorbate60 1.2
Triethanolamine 0.1
【Dosage form example 3】Massage cream
The composition recorded according to following table 6 prepares massage cream (unit by conventional method:Weight %).
【Table 6】
Ingredient Content
Purified Water Surplus
Glycerine 8.0
Butanediol 4.0
Liquid Paraffin 45.0
Beta glucan 7.0
Carbomer 0.1
Cyanidin -3-O- glucosides and natto gum (embodiment 1) 1.0
Caprylic/capric triglyceride 3.0
Beeswax 4.0
Cetearyl glucoside 1.5
Sorbitan sesquioleate 0.9
Vaseline 3.0
Paraffin 1.5
【Dosage form example 4】Facial mask
The composition recorded according to following table 7 prepares facial mask (unit by conventional method:Weight %).
【Table 7】
Ingredient Content
Purified Water Surplus
Glycerine 4.0
Polyvinyl alcohol 15.0
Hyaluronan extraction object 5.0
Beta glucan 7.0
Allantoin 0.1
Cyanidin -3-O- glucosides and natto gum (embodiment 1) 0.5
Nonylbenzene ether 0.4
Polysorbate60 1.2
Ethyl alcohol 6.0
【Dosage form example 5】Ointment in skin preparations for extenal use
The composition recorded according to following table 8 prepares ointment (unit by conventional method:Weight %).
【Table 8】
Ingredient Content
Purified Water Surplus
Glycerine 8.0
Butanediol 4.0
Liquid Paraffin 15.0
Beta glucan 7.0
Carbomer 0.1
Cyanidin -3-O- glucosides and natto gum (embodiment 1) 1.0
Caprylic/capric triglyceride 3.0
Saualane 1.0
Cetearyl glucoside 1.5
Stearic acid Isosorbide Dinitrate 0.4
Cetostearyl alcohol 1.0
Beeswax 4.0

Claims (10)

1. a kind of anti-aging Dermatologic preparation composition, the anti-aging Dermatologic preparation composition contain Cyanidin -3- O- glucosides (cyanidin-3-O-glucoside) and natto gum are as active ingredient.
2. a kind of skin elasticity improvement Dermatologic preparation composition, which contains Cyanidin -3-O- glucosides (cyanidin-3-O-glucoside) and natto gum are as active ingredient.
3. a kind of wrinkle of skin improvement Dermatologic preparation composition, which contains Cyanidin -3-O- glucosides (cyanidin-3-O-glucoside) and natto gum are as active ingredient.
4. according to the Dermatologic preparation composition described in any one of claim 1-3, which is characterized in that the natto gum is The hay bacillus (Bacillus subtilis) of activation or bacillus licheniformis (Bacillus licheniformis) are inoculated with It is fermented and is generated in soybean.
5. according to the Dermatologic preparation composition described in any one of claim 1-3, which is characterized in that the composition is logical It crosses and the expression of collagen catabolic enzyme is inhibited to play anti-aging effect.
6. according to the Dermatologic preparation composition described in any one of claim 1-3, which is characterized in that the corn flower The mixed weight of element -3-O- glucosides and natto gum ratio is 1:1~1:10.
7. according to the Dermatologic preparation composition described in any one of claim 1-3, which is characterized in that relative to composition Total weight, the contents of the Cyanidin -3-O- glucosides is 0.01~5 weight %;Relative to the total weight of composition, The content of the natto gum is 0.01~5 weight %.
8. according to the Dermatologic preparation composition described in any one of claim 1-3, which is characterized in that the external preparation for skin The chemical conversion of agent combination dosage form is selected from by smoothing toner, astringent lotion, nutrition toner, eye cream, nourishing cream, massage cream, cleansing cream, cleaning More than one form in the group that foam, clean water, loose powder, essence or facial mask form.
9. a kind of Cyanidin -3-O- glucosides and natto gum are as skin elasticity improver in Dermatologic preparation composition Purposes in preparation.
10. a kind of Cyanidin -3-O- glucosides and natto gum are as wrinkle of skin improver in Dermatologic preparation composition Preparation in purposes.
CN201680062930.7A 2016-09-28 2016-09-28 Anti-aging skin external composition containing cyanidin-3-O-glucoside and natto gum as effective components Active CN108348435B (en)

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Citations (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1803124A (en) * 2005-01-12 2006-07-19 东海生物科技股份有限公司 Gama-poly glutamic acid, gama-poly glutamic acid salt and use of hydrogel thereof
CN101370508A (en) * 2006-01-19 2009-02-18 玫琳凯有限公司 Compositions comprising kakadu plum extract or acai berry extract
KR20090056301A (en) * 2007-11-30 2009-06-03 (주)아모레퍼시픽 Cosmetic compositions for high moisturization
KR20110074189A (en) * 2009-12-24 2011-06-30 강원대학교산학협력단 Anthocyan derivative with improved structural stability and synthesis method for the same
KR20120126587A (en) * 2011-05-12 2012-11-21 가톨릭대학교 산학협력단 Anthocyanin via Charge Complex with Anionic Polysaccharide Having Improved Stability, Composition Containing the Same, and Method for Preparing the Same
CN103458872A (en) * 2010-12-30 2013-12-18 玫琳凯有限公司 Multi-purpose cosmetic compositions
KR20150019569A (en) * 2013-08-14 2015-02-25 주식회사 엘지생활건강 Composition for skin antioxidant, anti-imflamation, and skin whitening
CN104367539A (en) * 2014-11-25 2015-02-25 沈阳源康医疗器械有限公司 Conditioning lotion containing cordyceps militaris ingredients and preparation method thereof
CN105030560A (en) * 2015-07-13 2015-11-11 暨南大学 Protection effect of cyanidin-3-glucoside for UVB (ultraviolet B)-induced HaCaT cell injury
CN105125476A (en) * 2015-10-19 2015-12-09 广州科玛生物科技有限公司 Anti-wrinkle essence and preparing method thereof
JP2016049105A (en) * 2014-08-29 2016-04-11 花王株式会社 Method for producing solid dispersion containing difficult-to-dissolve polyphenol
CN105603012A (en) * 2015-12-11 2016-05-25 天津北洋百川生物技术有限公司 Preparation method for gamma-polyglutamic acid, and moisturizing face cream and preparation method thereof
KR20170025712A (en) * 2015-08-31 2017-03-08 (주)아모레퍼시픽 Skin external composition for anti-aging comprising cyanidin-3-o-glucoside and natto gum
CN108947955A (en) * 2018-08-08 2018-12-07 安徽省美极调味食品有限公司 The extraction process of functional food cyanidin extract

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI317291B (en) * 2005-01-12 2009-11-21 Tung Hai Biotechnology Corp Polyglutamic acid (gamma;-pga, h form), y-polyglutamates and gamma-polyglutamate hydrogels for use as super moisturizers in cosmetic and personal care products
CA2936822C (en) * 2006-01-19 2021-09-14 Mary Kay, Inc. Skin care compositions comprising kakadu plum extract

Patent Citations (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1803124A (en) * 2005-01-12 2006-07-19 东海生物科技股份有限公司 Gama-poly glutamic acid, gama-poly glutamic acid salt and use of hydrogel thereof
CN101370508A (en) * 2006-01-19 2009-02-18 玫琳凯有限公司 Compositions comprising kakadu plum extract or acai berry extract
KR20090056301A (en) * 2007-11-30 2009-06-03 (주)아모레퍼시픽 Cosmetic compositions for high moisturization
KR20110074189A (en) * 2009-12-24 2011-06-30 강원대학교산학협력단 Anthocyan derivative with improved structural stability and synthesis method for the same
CN103458872A (en) * 2010-12-30 2013-12-18 玫琳凯有限公司 Multi-purpose cosmetic compositions
KR20120126587A (en) * 2011-05-12 2012-11-21 가톨릭대학교 산학협력단 Anthocyanin via Charge Complex with Anionic Polysaccharide Having Improved Stability, Composition Containing the Same, and Method for Preparing the Same
KR20150019569A (en) * 2013-08-14 2015-02-25 주식회사 엘지생활건강 Composition for skin antioxidant, anti-imflamation, and skin whitening
JP2016049105A (en) * 2014-08-29 2016-04-11 花王株式会社 Method for producing solid dispersion containing difficult-to-dissolve polyphenol
CN104367539A (en) * 2014-11-25 2015-02-25 沈阳源康医疗器械有限公司 Conditioning lotion containing cordyceps militaris ingredients and preparation method thereof
CN105030560A (en) * 2015-07-13 2015-11-11 暨南大学 Protection effect of cyanidin-3-glucoside for UVB (ultraviolet B)-induced HaCaT cell injury
KR20170025712A (en) * 2015-08-31 2017-03-08 (주)아모레퍼시픽 Skin external composition for anti-aging comprising cyanidin-3-o-glucoside and natto gum
CN105125476A (en) * 2015-10-19 2015-12-09 广州科玛生物科技有限公司 Anti-wrinkle essence and preparing method thereof
CN105603012A (en) * 2015-12-11 2016-05-25 天津北洋百川生物技术有限公司 Preparation method for gamma-polyglutamic acid, and moisturizing face cream and preparation method thereof
CN108947955A (en) * 2018-08-08 2018-12-07 安徽省美极调味食品有限公司 The extraction process of functional food cyanidin extract

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
DARREN COOKE: "Effect of cyanidin-3-glucoside and an anthocyanin mixture from bilberry on adenoma development in the ApcMin mouse model of intestinal carcinogenesis—Relationship with tissue anthocyanin levels", 《INTERNATIONAL JOURNAL OF CANCER》 *
IVANA SERRAINO等: "Protective effects of cyanidin-3-O-glucoside from blackberry extract against peroxynitrite-induced endothelial dysfunction and vascular failure", 《LIFE SCIENCES》 *
MASSIMILIANO GASPARRINI等: "A Pilot Study of the Photoprotective Effects of Strawberry-Based Cosmetic Formulations on Human Dermal Fibroblasts", 《INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES》 *
佚名: "产品名称:丝滑纳豆胶", 《HTTPS://ONLYSTAR.COM/SHOW_1.PHP?CID=54&ID=92》 *
刘占云,等: "HPLC 法测定不同产地黑豆皮中矢车菊素-3-O-葡萄糖苷的含量", 《食品科学》 *
姚日生: "《药用高分子材料》", 30 April 2008, 化学工业出版社 *
裘炳毅: "《现代化妆品科学与技术》", 31 March 2016, 中国轻工业出版社 *

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MY196008A (en) 2023-03-06

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