CN108338981A - Application of the Actein in preparing anti-Cryptosporidium baileyi drug - Google Patents
Application of the Actein in preparing anti-Cryptosporidium baileyi drug Download PDFInfo
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- CN108338981A CN108338981A CN201810082303.0A CN201810082303A CN108338981A CN 108338981 A CN108338981 A CN 108338981A CN 201810082303 A CN201810082303 A CN 201810082303A CN 108338981 A CN108338981 A CN 108338981A
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- Prior art keywords
- actein
- drug
- cryptosporidium
- chick
- cryptosporidium baileyi
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
- A61K31/47—Quinolines; Isoquinolines
- A61K31/4738—Quinolines; Isoquinolines ortho- or peri-condensed with heterocyclic ring systems
- A61K31/4741—Quinolines; Isoquinolines ortho- or peri-condensed with heterocyclic ring systems condensed with ring systems having oxygen as a ring hetero atom, e.g. tubocuraran derivatives, noscapine, bicuculline
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/116—Heterocyclic compounds
- A23K20/132—Heterocyclic compounds containing only one nitrogen as hetero atom
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
- A61P33/02—Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Polymers & Plastics (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Animal Husbandry (AREA)
- Epidemiology (AREA)
- Food Science & Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention discloses the application of Actein and its pharmaceutically acceptable derivates in preparing anti-Cryptosporidium baileyi drug.By Actein and acceptable auxiliary material, powder is made, is added in feed, mixing, by daily forage volume feeding chick；Or Actein water solube powder is made in Actein, and it is soluble in water, by everyday water-drinking amount feeding chick so that the metainfective egg sac number of Cryptosporidium baileyi reduces 71% or more in chick.The experiment confirms that Actein has the function of preferable anti-Cryptosporidium baileyi.Meanwhile Actein is easily prepared, extraction cost is relatively low, is easy to exploitation as the anti-Cryptosporidium baileyi drug or feed addictive of low cost.
Description
Technical field
The present invention relates to the new opplication of a kind of compound, more particularly to Actein is preparing anti-Cryptosporidium baileyi drug
In application.
Background technology
Fowl Cryptosporidiosis is parasitized by Cryptosporidium baileyi, the Cryptosporidium meleagridis of Cryptosporidium section Cryptosporidium
Caused a kind of protozoosis in the respiratory system of poultry, alimentary canal, the bursa of farbricius and cloaca, Cryptosporidiosis is the second of fowl
The most common protozoosis in position.The Cryptosporidium for parasitizing birds at present has 3 to generally acknowledge effective kind：Cryptosporidium baileyi, turkey are hidden
Sporozoite and chicken Cryptosporidium.Wherein Cryptosporidium baileyi is the sociales for infecting poultry, larynx, gas of the main parasitic in poultry
The positions such as pipe, the bursa of farbricius and cloaca, cause respiratory tract, Alimentary tract disease, cause production performance to decline even dead.When adjoint
Inhibitive ability of immunity disease or when with other respiratory tracts, alimentary canal cause of disease coinfection, it will cause even more serious economic loss.It is hidden
Sporidiosis is in worldwide distribution, and Cryptosporidium is a kind of more host's parasitic protozoas.China be found in chicken, duck, goose, turkey,
In the bird class body such as quail, peacock, dove, sparrow, parrot, canary.Research report Guangdong duck infection rate be 85.67%~
100%；Henan duck infection rate is 9.8%, and total infection rate of the hidden spore of chicken is 18.8%, and the chicken infection rates of 2 week old is up to 42.5%；
Xi'an region Chickens Infected rate is 21.48%；The infection rate of Anhui chicken is 33.6%.Cryptosporidium baileyi mainly encroaches on 50 ages in days
Chick neurological susceptibility highest within chick below, especially 10 ages in days, morbidity and death, which are mainly seen within 10 ages in days, to be infected
Chick.In addition to slim egg capsule causes autoinfection in host, main infection mode is the bird class of morbidity and recessive band
Egg capsule in worm person's excrement polluted the feed of fowl, drinking-water etc. through infection of digestive canal, in addition also can be through respiratory tract infection.Morbidity nothing
It is apparent seasonal but multiple with warm rainy 8~September part, it is easy prevalence in the poor area of sanitary condition.Collect with livestock and poultry
The propulsion of reduction cultivation, cultivation density constantly increase, and the processing shortage of sewage, excrement caused by livestock and poultry cultivation is cost-effective
Purification techniques easily causes seriously to pollute to external environment.Cryptosporidium parvum oocysts suspended has stronger resistance, and traditional disinfection technique is very
Difficulty is killed, and cross transmission even breaks out risk between polluted source easily causes poultry-farm.However currently, controlling for this disease
Treatment there is no ideal active drug.Document report allicin, paromomycin treatment, there is certain curative effect.
Actein is a kind of tetracyclic triterpenoids compound, is naturally occurring in Lauraceae or Rattleroot plant roots and stems.It is civil normal
It is used as medicine with its rhizome, there is expelling wind and activating blood flow, bring down a fever and analgesic activities effect.Also studies have reported that Actein can be by preventing line
Plastochondria electronics transfer shows anti-vertebra worm activity, and has preferable correlation with antioxidant activity.In recent years also researches show that
It is increasingly valued by people with the bioactivity such as anti-inflammatory, antitumor.There has been no Acteins with anti-so far
The report of Cryptosporidium baileyi effect.
Invention content
It is an object of the invention to application of the Actein in preparing anti-Cryptosporidium baileyi drug.
The technical solution used in the present invention is：
The application of Actein and its pharmaceutically acceptable derivates in preparing anti-Cryptosporidium baileyi drug.
The application of Actein and its pharmaceutically acceptable derivates in preparing anti-Cryptosporidium baileyi egg capsule drug.
The application of Actein and its pharmaceutically acceptable derivates in preparing feed addictive.
A kind of drug of anti-Cryptosporidium baileyi contains Actein or its pharmaceutically acceptable derivative in the drug
Object.
The beneficial effects of the invention are as follows：By the way that by Actein and acceptable auxiliary material, powder is made, it is added to feed
In, mixing, by daily forage volume feeding chick；Or Actein water solube powder is made in Actein, it is soluble in water, per diem
Normal amount of drinking water feeding chick.Zoogenetic infection experiment confirms that addition Actein has the function of preferable anti-Cryptosporidium.Meanwhile
Actein is easily prepared, and extraction cost is relatively low, is easy to exploitation as the anti-Cryptosporidium baileyi drug or feed of low cost
Additive.
Specific implementation mode
Embodiment 1
The preparation of Actein powder：2.6g Acteins (content is not less than 98%) and 97.4g starch mixings are taken, i.e.,
It can.Actein is purchased from Shanghai Yuan Ye companies, article No.：B30166.
The usage and dosage of powder：Above-mentioned 100g powders are admixed in 100kg feeds, per diem often feeding amount feeding.
Embodiment 2
The preparation of Actein aqueous solvent：1.7g Acteins (content is not less than 98%) and 98.3g glucose mixings are taken,
.
The usage and dosage of water solube powder：Above-mentioned 100g pulvis is dissolved in 200 liters of water, is cut off the water supply to test chicken before medication
2 hours, then press normal water amount feeding.
It is tested with reference to the anti-Cryptosporidium baileyi of specific description of test Actein.
Experiment material：
Control drug：Document report with anti-Cryptosporidium effect paromomycin (98%, Shanghai source leaf biotechnology
Co., Ltd, article No.：B21484), usage amount is 5mg/kg weight.
Actein：The Actein powder of embodiment 1, the Actein water solube powder of embodiment 2.
Cryptosporidium baileyi egg capsule：Cryptosporidium baileyi (C.baileyi) Guangdong Strain egg capsule, by Guangdong Academy of Agricultural Sciences
Animal health research institute preserves, and using the preceding rejuvenation in SPF chicken bodies, (SPF chickens are purchased from Guangdong great Hua Nong inventories Co., Ltd, product
Kind：BWEL-SPF chickens).
Chick：Lingnan Yellow chick, 1 age in days, male and female dual-purpose are provided by animal science research institute of Guangdong Academy of Agricultural Sciences,
It raises in the special animal house sterilized；Chicken coop and the equal strict sterilization of vessel used, free choice feeding and drinking pure water.Experiment
Preceding observation chick whether there is or not clinical symptoms and continuous 3d checks excrement, and whether there is or not Cryptosporidium parvum oocysts suspendeds, spare.
Feed：Material of brooding is customized by Guangdong Province Xin Nan all feed corporation,Ltds, is free of any anti-Cryptosporidium drug.
Experimental method：
Grouping：By experiment grouping and handle 150 Lingnan Yellow breeding chicklings to 3 ages in days, by only weighing, eliminate it is weak young and
Remaining chick is randomly divided into 5 groups by the excessive person of weight, every group 30, and suitably adjustment keeps every group of chick total weight roughly equal.
Processing：In addition to I (blank control) is organized, equal administered by oral gavage Cryptosporidium baileyi egg capsule l × 10 of remaining each group chick6
A/plumage；In addition to Group I and the IIth (positive control) are organized, remaining each group chick is fed or is drunk immediately after infection containing anti-hidden
The Actein of the feed or water of sporozoite drug, the IIIth group of feed of the feeding containing paromomycin, the IVth group of feeding embodiment 1 dissipates
Made of Actein spice made of agent and feed, the Vth group of Actein water solube powder for drinking embodiment 2 and drinking-water
Actein is drunk water, the dosage all same of each group drinking-water and feed.The grouping situation of experiment chick is shown in Table 1.
Table 1 tests chick grouping
Mental status, feed intake, the excrement situation etc. of chicken group are observed and recorded daily；It weighs to dead chick, dissect；Most
Weightening and the price of deed of each group chick are calculated afterwards.
Egg capsule slip after the infection of drug effect determination method Main Basiss；It is following to carry out：Start daily collect within the 3rd day after infection
Excrement, continuous monitoring 15 days.Take excrement to eluriate daily, respectively with 40,80,100 mesh screen filtrations, filtrate through 3000r/min from
Heart 10min is abandoned and is reset and added 15mL saturation sucrose solutions, shakes up, 3000/min centrifuges 10min；It is careful immediately after centrifugation to draw
In on blood cell counting plate, (10 × 40 times) observation egg capsules of microscope simultaneously count clear liquid 10uL.
Experimental result：
Clinicing symptom observation：
It is inoculated with the control group of Cryptosporidium baileyi egg capsule the 3rd day, chick appearance spirit is depressed, and hair is slightly random, abdomen from the 5th day
It is apparent to rush down symptom, during which has no chick death.
Average every chick row egg sac number, egg capsule slip and the average weight for calculating separately each group, the results are shown in Table 2 institutes
Show.As shown in Table 2, the III, the IV, V group of average every chick row egg sac number is below the IIth group, and average weight is above the
II groups；IV, the V group of average every chick row egg sac number is significantly lower than the IIIth group, and average weight is higher than Group III.Therefore it tests
Confirm that Actein can make the metainfective egg sac number of Cryptosporidium baileyi reduce 71% or more, the price of deed increases, drinking-water
Group effect is slightly above feed addition group, has certain anti-Cryptosporidium baileyi effect, and effect is better than paromomycin.
The anti-Cryptosporidium effect of 2 each group of table
Claims (4)
1. the application of Actein and its pharmaceutically acceptable derivates in preparing anti-Cryptosporidium baileyi drug.
2. the application of Actein and its pharmaceutically acceptable derivates in preparing anti-Cryptosporidium baileyi egg capsule drug.
3. the application of Actein and its pharmaceutically acceptable derivates in preparing feed addictive.
4. a kind of drug of anti-Cryptosporidium baileyi, which is characterized in that contain Actein in the drug or it pharmaceutically may be used
The derivative of receiving.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810082303.0A CN108338981B (en) | 2018-01-29 | 2018-01-29 | Actein is preparing the application in anti-Cryptosporidium baileyi drug |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810082303.0A CN108338981B (en) | 2018-01-29 | 2018-01-29 | Actein is preparing the application in anti-Cryptosporidium baileyi drug |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108338981A true CN108338981A (en) | 2018-07-31 |
| CN108338981B CN108338981B (en) | 2019-04-16 |
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|---|---|---|---|
| CN201810082303.0A Active CN108338981B (en) | 2018-01-29 | 2018-01-29 | Actein is preparing the application in anti-Cryptosporidium baileyi drug |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050008717A1 (en) * | 2002-12-27 | 2005-01-13 | Einbond Linda Saxe | Anti-neoplastic compositions comprising extracts of black cohosh |
| CN102432617A (en) * | 2011-12-04 | 2012-05-02 | 苏州派腾生物医药科技有限公司 | Method for extracting actinodaphnine from actinodaphne obovata |
| CN108186656A (en) * | 2017-12-26 | 2018-06-22 | 合肥工业大学 | A kind of new application of cimicifugae foetidae and its extract |
-
2018
- 2018-01-29 CN CN201810082303.0A patent/CN108338981B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050008717A1 (en) * | 2002-12-27 | 2005-01-13 | Einbond Linda Saxe | Anti-neoplastic compositions comprising extracts of black cohosh |
| CN102432617A (en) * | 2011-12-04 | 2012-05-02 | 苏州派腾生物医药科技有限公司 | Method for extracting actinodaphnine from actinodaphne obovata |
| CN108186656A (en) * | 2017-12-26 | 2018-06-22 | 合肥工业大学 | A kind of new application of cimicifugae foetidae and its extract |
Non-Patent Citations (1)
| Title |
|---|
| 赵奇志等: "阿朴菲类生物碱生物活性研究进展", 《天然产物研究与开发》 * |
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| Publication number | Publication date |
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| CN108338981B (en) | 2019-04-16 |
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Effective date of registration: 20210317 Address after: 526300 liujingzui village, hongtaiyang village committee, Nanjie Town, Guangning County, Zhaoqing City, Guangdong Province (500m opposite the village committee) Patentee after: Guangdong xinlinghang Ecological Agriculture Development Co.,Ltd. Address before: 510640 No.21 baishigang street, Wushan, Tianhe District, Guangzhou City, Guangdong Province Patentee before: INSTITUTE OF ANIMAL HEALTH, GUANGDONG ACADEMY OF AGRICULTURAL SCIENCES |