CN108300733B - Method for identifying signal peptide with secretion function based on pathogen-related molecular pattern protein - Google Patents
Method for identifying signal peptide with secretion function based on pathogen-related molecular pattern protein Download PDFInfo
- Publication number
- CN108300733B CN108300733B CN201810055204.3A CN201810055204A CN108300733B CN 108300733 B CN108300733 B CN 108300733B CN 201810055204 A CN201810055204 A CN 201810055204A CN 108300733 B CN108300733 B CN 108300733B
- Authority
- CN
- China
- Prior art keywords
- signal peptide
- pathogen
- protein
- xeg1
- expression vector
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8205—Agrobacterium mediated transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Nutrition Science (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention provides a method for identifying signal peptide with secretion function based on pathogen-related molecular pattern protein, which comprises the following steps: (1) fusing a signal peptide to be identified with the C-terminal of the phytophthora sojae XEG1 to construct an expression vector, wherein the amino acid sequence of the phytophthora sojae XEG1 is shown as SEQ ID No. 1; (2) transforming the expression vector constructed in the step (1) into competent cells; (3) injecting the competent cells obtained in the step (2) into plant cells, observing the necrosis condition of the plant cells at the injection site, wherein the signal peptide to be detected has a secretion function if the plant cells at the injection site are necrotic, and the signal peptide to be detected does not have the secretion function if the plant cells at the injection site are not necrotic.
Description
Technical Field
The invention belongs to the technical field of rapid detection of secretory protein signal peptide, and particularly relates to a method for rapidly verifying secretory protein signal peptide on plant cells by utilizing the characteristic that XEG1 causes necrosis outside the cells.
Background
Secreted proteins are proteins that are synthesized intracellularly and secreted to function extracellularly. The signal peptide is located at the N-terminal of the secreted protein and generally consists of 15-30 amino acids, and under the guidance of the signal peptide, the newly synthesized protein is secreted to the outside of the cell to play a role. The pathogen-associated molecular pattern protein XEG1 of phytophthora sojae, a plant pathogen, can cause cell necrosis on tobacco in the presence of signal peptide, and cannot cause necrosis when the signal peptide is removed. Therefore, the signal peptide part to be detected is fused with XEG 1C end without signal peptide, and is expressed in cells, if the signal peptide to be detected is functional, XEG1 can be guided to the outside of the cells, and cell necrosis is caused; if the signal peptide to be detected is not functional, it cannot be directed XEG1 outside the cell to cause necrosis.
Currently, there are methods for detecting the function of a secretion signal peptide, such as the pSUC2 system in yeast, or the overexpression and detection of a secretion protein fusion tag protein in pathogenic bacteria. The yeast system needs yeast transformation and is verified on various culture media, and the operation process is complicated and the cost is high. The secretory protein fused with the label protein is overexpressed in pathogenic bacteria, the transformation of the pathogenic bacteria is needed, the efficiency is low, the period is long, and the cost is high. The prior art needs a method for detecting a signal peptide with a secretion function, which has the advantages of simple operation, low cost, high accuracy, high efficiency and short time consumption.
Disclosure of Invention
The invention aims to provide a method for identifying whether a signal peptide has a secretion function or not, which has the advantages of low cost, high accuracy, low cost and short time consumption. The invention utilizes the characteristic that secretory protein XEG1 causes cell death outside plant cells to fuse the signal peptide to be detected and XEG1 de-signal peptide part, expresses in the plant cells and observes whether necrosis is caused, and can quickly and efficiently detect whether the signal peptide has a secretory function.
The invention provides a method for identifying signal peptide with secretion function based on pathogen-related molecular pattern protein, which comprises the following steps:
(1) fusing a signal peptide to be identified with the C end of the pathogen-associated molecular pattern protein signal-free peptide to construct an expression vector;
(2) transforming the expression vector constructed in the step (1) into competent cells;
(3) injecting the competent cells obtained in the step (2) into plant cells, observing the necrosis condition of the injection site, wherein the signal peptide to be detected has a secretion function if the cells at the injection site are necrotic, and the signal peptide to be detected does not have the secretion function if the cells at the injection site are not necrotic.
The pathogen-related molecular pattern protein is phytophthora sojae XEG1, the amino acid sequence is shown in SEQ ID NO.1, and the nucleotide sequence is shown in SEQ ID NO. 2.
The expression vector in the step (1) is a plant transient expression vector.
And (3) enabling the competent cells in the step (2) to be agrobacterium-infected competent cells.
The plants in the step (3) are tobacco, potato, tomato and pepper.
The method for identifying the signal peptide with the secretion function based on the pathogen-related molecular pattern protein further comprises the step of setting a positive control, wherein the positive control is obtained by connecting the signal peptide of the phytophthora sojae non-toxic effect protein Avr1b with the C end of the pathogen-related molecular pattern protein non-signal peptide to construct an expression vector in the step (1).
Preferably, the positive control is obtained by connecting the signal peptide of Avr1b with the amino acid sequence shown as SEQ ID NO.3 with the C-terminal of phytophthora sojae XEG1 without the signal peptide with the amino acid sequence shown as SEQ ID NO.1 to construct a plant transient expression vector.
The invention provides application of the method in screening signal peptides with secretion functions.
Further, signal peptides having a secretory function can be selected by the method of the present invention, and those skilled in the art can use these signal peptides for heterologous gene expression.
Based on this, the invention provides the application of the method in the preparation of transgenic plants.
The method has the advantages that the defects of large workload, high cost, long period and the like in the prior art for identifying the secretory protein signal peptide are overcome, based on the characteristic of XEG1 necrosis caused outside cells, the function of the signal peptide to be identified can be identified conveniently and quickly by a method for instantly expressing the signal peptide in plant cells, the operation identification is short in time consumption, low in cost and accurate and reliable in result, the requirement of the function identification of the signal peptide can be met well, and the method has wide application prospects in the technical field of plant genetic engineering.
Drawings
FIG. 1 is a diagram showing the secretion function of a signal peptide as verified by XEG 1. The signal peptide fusion XEG1 of Avr1b, SsSSVP1, GME5899, AvrPiz-t, the de-signal peptide portion was subjected to agrobacterium-mediated transient expression on tobacco, and whether the injection site was necrotic was observed after 4-6 days, with XEG1 full length as a control. Grey indicates the signal peptide for Avr1b, SssVP1, GME5899, AvrPiz-t, or XEG 1.
Detailed Description
The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
Example 1
(1) A signal peptide sequence (amino acid sequence is shown as SEQ ID NO.3) of phytophthora sojae non-toxic effector protein Avr1b and a signal peptide sequence (amino acid sequence is shown as SEQ ID NO.4) of Sclerotinia sclerotiorum secretion protein SSSVP1 are respectively connected with a C end of phytophthora sojae XEG1 without a signal peptide, which is shown as SEQ ID NO.1, to construct a plant transient expression vector, and a vector capable of expressing Avr1bSP-XEG1 and SSSVP1SP-XEG1 on a plant is obtained.
The specific operation is as follows: designing specific primers according to the sequence of the fragment, and amplifying a signal peptide region of the Avr1B by using SP1bF (SEQ ID NO.7) and LapSP1B-XEG1R (SEQ ID NO.8) to obtain an amplification product A, and amplifying a C-terminal region of XEG1 by using LapSP1B-XEG1F (SEQ ID NO.9) and XEG1R (SEQ ID NO.10) to obtain an amplification product B; SP-SssVP 1F (SEQ ID NO: 11) and LapSP-SssVP 1-XEG1R (SEQ ID NO: 12) amplify the signal peptide region of SssVP1 to obtain amplification product C, and LapSP-SssVP 1-XEG1F (SEQ ID NO: 13) and XEG1R amplify the C-terminal region of SssVP1 to obtain amplification product D. PCR amplification was performed as follows: pre-denaturation at 94 ℃ for 3 min; denaturation at 94 ℃ for 30s, annealing at 58 ℃ for 30s, and extension at 72 ℃ for 60s for 30 cycles; extension at 72 ℃ for 10 min. After the amplification is finished, carrying out electrophoresis on 1% agarose gel to obtain a DNA amplification band with the length of a target size; and (4) recovering and purifying by using a gel recovery kit. Since the signal peptide regions of Avr1B and SsSSVP1 were fused to the C-terminus of XEG1, respectively, PCR amplification was performed using SP1bF and XEG1R primers and a fragment mixture of amplification products a and B as templates to obtain Avr1B signal peptide and a C-terminal fused fragment Avr1bSP-XEG1 of XEG 1; PCR amplification was performed using SP-SssVP 1F and XEG1R primers and a fragment mixture of amplification products C and D as templates to obtain an SssVP1 signal peptide and a C-terminal fusion fragment of XEG1, SssVP1SP-XEG 1. And (4) recovering and purifying by using a gel recovery kit. The expression vector is subjected to double enzyme digestion by Cla I and SmaI enzymes, and the enzyme digestion product is recovered by a gel recovery kit. The gene fragment was ligated to the vector using a one-step ligation kit (cat # C112-01, Vazyme Biotech Co., Ltd.), transformed E.coli JM109, positive clones were selected, verified by PCR and by restriction enzyme digestion of the extracted plasmid, and submitted to the company for sequencing verification.
(2) Agrobacterium containing the target vector is obtained by a method of transforming agrobacterium by electric shock. The specific operation is as follows: agrobacterium transformation was performed on an electrical transformation apparatus, and the electric shock cup and cup holder were placed on ice. The parameters of the electric converter were set, the capacitance C was 25 μ F and the voltage V was 2.5kV (electric cup was 0.2 cm). After the electrotransfer was completed, 1ml of LB medium was added to the cuvette, the cells were resuspended and transferred to an EP tube and cultured with shaking at 30 ℃ for 1-2 h. Centrifuging to collect cells, discarding supernatant, reserving 50-100 μ l of liquid, lightly and uniformly mixing and coating on an LB plate containing kanamycin, culturing at 30 ℃, and after single colony grows out, picking single colony for positive colony verification.
(3) Agrobacterium containing the Avr1bSP-XEG1 and SssVP1SP-XEG1 expression vectors were co-injected in tobacco, respectively, and whether the injection site was necrotic was observed after 4-6 days. Selecting Agrobacterium containing the desired gene plasmid, culturing in LB liquid medium containing kanamycin (50. mu.g/ml) under shaking at 30 deg.C for 24 hr, centrifuging at 3000rpm for 3min, collecting thallus, and adding MgCl2(10m M) the solution was resuspended in water and 3 times repeated, and then resuspended. The OD 600 was fixed 0.3 for tobacco injection. Selecting the Nicotiana benthamiana grown in a greenhouse for 4-6 weeks, and selecting a proper tobacco leaf for injection inoculation of agrobacterium. The syringe needle caused minimal trauma to the tobacco lower epidermis and the agrobacterial suspension was infiltrated into the Nicotiana benthamiana leaves using a needleless syringe. Agrobacterium containing Avr1bSP-XEG1 and SssVP1SP-XEG1 were injected on tobacco, respectively, while Agrobacterium containing XEG1 was injected as a positive control, and whether the injection site was necrotic was observed after 4-6 days. The occurrence of necrosis indicates that the protein can be secreted outside the cell withoutNecrosis indicates that the protein cannot be secreted outside the cell and the signal peptide is not functional, as shown in FIG. 1. The results showed that both Avr1bSP-XEG1 and SsSSVP1SP-XEG1 were able to cause necrosis of plant cells, indicating that the signal peptides of Avr1b and ssvp1 have a secretory function.
Example 2
In order to verify the reliability of the method, the secretion function of a predicted secretory protein GME5899 in ustilaginoidea virens and a nontoxic effector protein AvrPiz-t signal peptide in pyricularia grisea are selected for detection. A plant transient expression vector is constructed by fusing the GME5899 ((amino acid sequence is shown as SEQ ID NO.5) and AvrPiz-t (amino acid sequence is shown as SEQ ID NO. 6)) signal peptide region and the de-signal peptide region of XGE1 by the method of (1) in example 1, agrobacterium of the gene expression vector of interest is co-injected in tobacco, and whether the injection site is necrotic is observed after 4-6 days.
Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Sequence listing
<110> university of agriculture in Huazhong
<120> method for identifying signal peptide with secretion function based on pathogen-associated molecular pattern protein
<130> KHP171118740.0
<160> 13
<170> SIPOSequenceListing 1.0
<210> 1
<211> 241
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 1
Met Lys Gly Phe Phe Ala Gly Val Val Ala Ala Ala Thr Leu Ala Val
1 5 10 15
Ala Ser Ala Gly Asp Tyr Cys Gly Gln Trp Asp Trp Ala Lys Ser Thr
20 25 30
Asn Tyr Ile Val Tyr Asn Asn Leu Trp Asn Lys Asn Ala Ala Ala Ser
35 40 45
Gly Ser Gln Cys Thr Gly Val Asp Lys Ile Ser Gly Ser Thr Ile Ala
50 55 60
Trp His Thr Ser Tyr Thr Trp Thr Gly Gly Ala Ala Thr Glu Val Lys
65 70 75 80
Ser Tyr Ser Asn Ala Ala Leu Val Phe Ser Lys Lys Gln Ile Lys Asn
85 90 95
Ile Lys Ser Ile Pro Thr Lys Met Lys Tyr Ser Tyr Ser His Ser Ser
100 105 110
Gly Thr Phe Val Ala Asp Val Ser Tyr Asp Leu Phe Thr Ser Ser Thr
115 120 125
Ala Ser Gly Ser Asn Glu Tyr Glu Ile Met Ile Trp Leu Ala Ala Tyr
130 135 140
Gly Gly Ala Gly Pro Ile Ser Ser Thr Gly Lys Ala Ile Ala Thr Val
145 150 155 160
Thr Ile Gly Ser Asn Ser Phe Lys Leu Tyr Lys Gly Pro Asn Gly Ser
165 170 175
Thr Thr Val Phe Ser Phe Val Ala Thr Lys Thr Ile Thr Asn Phe Ser
180 185 190
Ala Asp Leu Gln Lys Phe Leu Ser Tyr Leu Thr Lys Asn Gln Gly Leu
195 200 205
Pro Ser Ser Gln Tyr Leu Ile Thr Leu Glu Ala Gly Thr Glu Pro Phe
210 215 220
Val Gly Thr Asn Ala Lys Met Thr Val Ser Ser Phe Ser Ala Ala Val
225 230 235 240
Asn
<210> 2
<211> 726
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 2
atgaagggat tcttcgccgg tgtcgtcgct gctgccaccc tcgcggtcgc ctccgctgga 60
gactactgcg gccagtggga ctgggccaag agcactaact acatcgtgta caacaacctc 120
tggaacaaga acgccgccgc atcgggcagc caatgcacgg gcgtcgacaa gatcagcggc 180
tccaccatcg cctggcacac gtcgtacacc tggacgggag gcgcggccac ggaggtcaag 240
tcgtactcga acgccgcgct ggtcttctcc aagaagcaga tcaagaacat caagtcgatc 300
cccaccaaga tgaagtactc gtactcgcac tcctcgggca cgttcgtcgc tgacgtgtcg 360
tacgacctgt tcacgagctc caccgccagc ggcagcaacg agtacgagat catgatctgg 420
ctggccgcgt acggcggcgc cggcccgatc tccagcacgg gcaaggccat cgccaccgtc 480
accatcggca gcaacagctt caagctgtac aagggcccga acggcagcac caccgtcttc 540
tcgttcgtcg ccaccaagac catcaccaac ttctcggccg acctgcagaa gttcctgagc 600
tacctcacca agaaccaggg cctgccttcc agccagtacc tcatcacgct cgaggccggc 660
acggagccgt tcgtgggcac gaacgccaag atgaccgtat cctcgttctc ggctgcggtc 720
aactga 726
<210> 3
<211> 21
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 3
Met Arg Leu Ser Phe Val Leu Ser Leu Val Val Ala Ile Gly Tyr Val
1 5 10 15
Val Thr Cys Asn Ala
20
<210> 4
<211> 22
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 4
Met Lys Val Val Leu Phe Ile Ser Ala Leu Ala Ala Thr Leu Ser Ala
1 5 10 15
Ala Ala Pro Leu Glu Pro
20
<210> 5
<211> 25
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 5
Met Lys Thr Ser Val Val Ala Leu Ala Ala Leu Leu Gly Leu Ala Ser
1 5 10 15
Ala Gln Asn Ala Val Val Ile Asn Ser
20 25
<210> 6
<211> 25
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 6
Met Gln Phe Ser Thr Ile Ile Thr Val Cys Leu Phe Thr Gly Leu Ala
1 5 10 15
Ser Ala Ser Phe Val Gln Cys Asn His
20 25
<210> 7
<211> 38
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 7
cagcaccagc tagcatcgat atgcgtctat cttttgtg 38
<210> 8
<211> 38
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 8
cactggccgc agtagtctcc tgcgttgcag gtcacgac 38
<210> 9
<211> 38
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 9
gtcgtgacct gcaacgcagg agactactgc ggccagtg 38
<210> 10
<211> 38
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 10
agtccatgga tcccccgggg ttgaccgcag ccgagaac 38
<210> 11
<211> 41
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 11
cagcaccagc tagcatcgat atgaaggtcg ttctgtttat c 41
<210> 12
<211> 41
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 12
cactggccgc agtagtctcc tggctctagt ggggcagctg c 41
<210> 13
<211> 41
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 13
gcagctgccc cactagagcc aggagactac tgcggccagt g 41
Claims (6)
1. A method for identifying a signal peptide with a secretion function based on a pathogen-associated molecular pattern protein is characterized in that,
by utilizing the characteristic that secretory protein XEG1 causes cell death outside plant cells, the signal peptide to be detected and XEG1 de-signal peptide are partially fused, expressed in the plant cells and observed whether necrosis is caused, so that whether the signal peptide has a secretory function is quickly and efficiently detected;
the method comprises the following steps:
(1) fusing a signal peptide to be identified with the C end of the pathogen-associated molecular pattern protein signal-free peptide to construct an expression vector;
the pathogen-related molecular pattern protein is phytophthora sojae XEG1, the amino acid sequence of the pathogen-related molecular pattern protein is shown as SEQ ID No.1, and the nucleotide sequence of the pathogen-related molecular pattern protein is shown as SEQ ID No. 2; the expression vector is a plant transient expression vector;
(2) transforming the expression vector constructed in the step (1) into competent cells, wherein the competent cells are agrobacterium-infected competent cells;
(3) injecting the competent cells obtained in the step (2) into plant cells, observing the necrotic condition of the injection site, wherein the signal peptide to be detected has a secretion function if the plant cells at the injection site are necrotic, and the signal peptide to be detected does not have the secretion function if the plant cells at the injection site are not necrotic.
2. The method of claim 1, wherein the plant of step (3) is tobacco, potato, tomato, pepper.
3. The method as claimed in claim 1, further comprising setting up a positive control, wherein the positive control is obtained by connecting the signal peptide of the avirulent effector protein Avr1b of Phytophthora sojae to the C-terminal of the pathogen-associated molecular pattern protein non-signal peptide to construct an expression vector at the stage of step (1).
4. The method of claim 3, wherein the positive control is obtained by constructing a plant transient expression vector by linking a signal peptide of Avr1b having an amino acid sequence as set forth in SEQ ID No.3 to a C-terminus of Phytophthora sojae XEG1 having an amino acid sequence as set forth in SEQ ID No.1, which does not have a signal peptide.
5. Use of the method of any one of claims 1 to 4 for screening for signal peptides with secretory function.
6. Use of the method of any one of claims 1-4 for the preparation of transgenic plants.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810055204.3A CN108300733B (en) | 2018-01-19 | 2018-01-19 | Method for identifying signal peptide with secretion function based on pathogen-related molecular pattern protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810055204.3A CN108300733B (en) | 2018-01-19 | 2018-01-19 | Method for identifying signal peptide with secretion function based on pathogen-related molecular pattern protein |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108300733A CN108300733A (en) | 2018-07-20 |
| CN108300733B true CN108300733B (en) | 2021-09-24 |
Family
ID=62865986
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810055204.3A Active CN108300733B (en) | 2018-01-19 | 2018-01-19 | Method for identifying signal peptide with secretion function based on pathogen-related molecular pattern protein |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108300733B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110156885B (en) * | 2019-05-23 | 2020-11-24 | 南京林业大学 | Pathogenic-related mode molecular protein BxCDP1 of pine wood nematode and application thereof |
| CN112940135A (en) * | 2019-12-11 | 2021-06-11 | 四川农业大学 | Fusion protein, amino acid sequence, coding nucleotide sequence, preparation method and application thereof |
-
2018
- 2018-01-19 CN CN201810055204.3A patent/CN108300733B/en active Active
Non-Patent Citations (5)
| Title |
|---|
| A genetic selection for isolating cDNAs encoding secreted proteins;K A Jacobs et al;《Gene》;19971001;第198卷(第1-2期);289-296 * |
| A phytophthora sojae glycoside hydrolase 12 protein is a major virulence factor during soybean infection and is recognized as a PAMP;Zhenchuan Ma et al;《The Plant Cell》;20150710;第27卷(第7期);2057-2072 * |
| Identification of eukaryotic secreted and cell surface proteins using the yeast secreting trap screen;Sang-Jik Lee et al;《Nature Protocols》;20061229;第1卷(第5期);2439-2447 * |
| 大豆疫霉病原相关模式分子PsXEG1的免疫活性和毒性功能分析;朱林;《万方数据知识服务平台》;20171219;全文 * |
| 疫霉属一个新病原相关模式分子PsXEG1的鉴定和功能分析;马振川;《中国优秀硕士学位论文全文数据库 农业科技辑 D046-14》;20170615(第6期);D046-14 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108300733A (en) | 2018-07-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107722125B (en) | Artificial transcription activator dCas9-TV and coding gene and application thereof | |
| CN104946665B (en) | GmMYB62Application in cultivating transgenic plant with improved stress resistance | |
| CN110713526B (en) | Wheat stress-resistant protein TaBZR2D and coding gene and application thereof | |
| CN108300733B (en) | Method for identifying signal peptide with secretion function based on pathogen-related molecular pattern protein | |
| CN102140443B (en) | Plant stress-resistant associated protein, and encoding gene and application thereof | |
| CN112424366A (en) | Recombinant vector for producing antigen for diagnosing African swine fever and use thereof | |
| CN108359689B (en) | Method for identifying signal peptide with secretion function by using disease-resistant gene to identify nontoxic effector protein | |
| CN101585871A (en) | Protein related to heat resistance property and coding gene and application thereof | |
| CN106978438B (en) | Method for improving homologous recombination efficiency | |
| CN101565461A (en) | Zinc finger protein related to plant type and spike grain number of rice, encoding gene and application thereof | |
| KR100803095B1 (en) | Gene coding chitosanase from bacillus subtilis ch2 expression vector comprising the gene transformant transfected with the expression vector and method for purification of protein produced by thereof | |
| CN103261419A (en) | Construct for regulating fertility of plant pollens and usage thereof | |
| US5362864A (en) | Trans-activating factor-1 | |
| CN113564200A (en) | Application of tea tree CsCIPK20 gene in improving cold resistance of plants | |
| CN110616230B (en) | Method for promoting secretory expression of zearalenone degrading enzyme ZHD protein and application | |
| CN111556898B (en) | Recombinant vector comprising porcine FC fragment and method for preparing recombinant protein by using same | |
| CN108727480B (en) | Transcription inhibition structure domain, coding gene and application thereof | |
| CN112538490A (en) | NLP gene for inducing necrosis and active oxygen accumulation in biocontrol pythium and expression vector and application thereof | |
| CN113717256A (en) | Fusion protein and application thereof | |
| CN112391367A (en) | Preparation method of Cas9 protein for gene editing of human primary cells | |
| US20060121564A1 (en) | Novel expression vector suitable for expression of recombinant protein at low temperature | |
| CN109354609A (en) | A kind of Salt Tolerance Gene in Alfalfa Drought-tolerant gene and its application | |
| CN116003563B (en) | Application of calmodulin binding protein CaMBP in regulating cold tolerance of plant | |
| CN107653253A (en) | One kind regulation and control Tobacco Flowering period NtMADS2 genes and its cloning process and application | |
| CN110484548B (en) | Method for constructing expression vector by one-step Gateway reaction and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |