CN108210542A - A kind of biological enzyme processing and corrosion-resistant Asian puccoon plant extraction liquid and preparation method thereof - Google Patents
A kind of biological enzyme processing and corrosion-resistant Asian puccoon plant extraction liquid and preparation method thereof Download PDFInfo
- Publication number
- CN108210542A CN108210542A CN201810296325.7A CN201810296325A CN108210542A CN 108210542 A CN108210542 A CN 108210542A CN 201810296325 A CN201810296325 A CN 201810296325A CN 108210542 A CN108210542 A CN 108210542A
- Authority
- CN
- China
- Prior art keywords
- extraction liquid
- asian puccoon
- plant extraction
- preparation
- parts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/30—Boraginaceae (Borage family), e.g. comfrey, lungwort or forget-me-not
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/02—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings containing insect repellants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/524—Preservatives
Abstract
The present invention provides a kind of biological enzyme processing and corrosion-resistant Asian puccoon plant extraction liquid and preparation method thereof.Described method includes following steps：(1) using alkanet as raw material, the alkanet block that length is less than preset value is obtained after pretreatment；(2) alkanet block made from step (1) is placed in 20~45 DEG C of soak and impregnated 15~30 minutes, soak includes 10~25 parts of complex enzyme formulation, 1~5 part of salts substances and 40~100 parts of water；(3) the alkanet block after immersion and soak are ultrasonically treated 1~2 hour, isolated supernatant after static layering；(4) using the chromatography column pure water elution for being filled with resin extender, impurity, eluent, that is, Asian puccoon plant extraction liquid of acquisition are removed.The preparation method recovery rate of the present invention is high, and the Asian puccoon plant extraction liquid active ingredient content of preparation is high, and mild non-stimulated, mosquito-proof sting, anti-inflammatory hemostasis can effectively go acne and desalination scar, and have natural anticorrosion performance.
Description
【Technical field】
The present invention relates to Asian puccoon active ingredient extractive technique field more particularly to a kind of biological enzyme processing and corrosion-resistant purple
Careless plant extraction liquid and preparation method thereof.
【Background technology】
Asian puccoon is clinical conventional Chinese medicine, from comfrey lithospermum euchromum Royle Arnebia euchroma (Royle)
Johnst, Asian puccoon Lithospermum erythrorhizon Sieb.et Zcc. or arnebia guttata Bunge Arnebia guttata
The dry root of Bunge is used with the effect of cool blood, promoting blood circulation, removing toxic substances promoting eruption.The active ingredient of Asian puccoon for the naphthoquinones class such as alkannin into
Point, pharmacological research shows that alkannin has the pharmacology promoting blood circulation such as antibacterial, anti-inflammatory, antiviral.
Except above-mentioned pharmacopeia collect Asian puccoon kind in addition to, also have at present as other substitutes use such as gromwell root
(Lithospermum erythrorhizon Sieb.et Zucc.), Onosma paniculatum (Onosma paniculatum Bur.et
Frach.), the kinds such as O.exsertumHemsl (O.exsertm Hemsl.), close colored Onosma paniculatum (O.confertum W.W.smith)
Root, in root skin or root cork, also containing alkannin and Gronwell naphthaquinone composition.
Report has the method for Asian puccoon raw material extraction alkannin at present, mainly there is supercritical CO2Extraction and organic solvent extraction
Deng.Such as publication number CN13314103A, CN1556087A Chinese patent literatures are reported with supercritical CO2The method of extraction,
The recovery rate of total extract is relatively low, and the utilization rate of raw material is low, in addition equipment limit, of high cost, it is more difficult to industrialized production.It is open
Number CN1607199A is described extracted using petroleum ether after, then successively hydrolyzed with 0.1~0.5M sodium hydroxide solutions, use
0.5~2M sulfuric acid is acidified to obtain Alkannin.Publication number CN1271745A is described using n-hexane, acetone, petroleum ether, second
After acetoacetic ester extraction Asian puccoon, it is concentrated under reduced pressure, is dried under reduced pressure the method for extracting natural red colouring matter.Publication number CN111750A is described
It is a kind of first to impregnate the alkanet crushed in 60~80 DEG C with 0.1~0.2% dilute hydrochloric acid, then with n-hexane in 20~35 DEG C of immersions
Extraction adds in dextrin after vacuum concentration to thick, and spray drying obtains.
Above-mentioned Asian puccoon extracting method is mainly single solvent extraction, then extract liquor concentration is alkalized, the production cycle
Long, alkannin content is low in extract, and product leads to Asian puccoon there are certain irritation, and since the technique of Hydrolysis kinetics is different
The content difference of Asian puccoon is larger in extraction, and the content of alkannin differs, can cause Radix Arnebiae extract pharmacological activity change compared with
Greatly.
Therefore, it is really necessary to provide a kind of new alkannin extracting method to solve above-mentioned technical problem.
【Invention content】
The purpose of the present invention is to provide it is a kind of with biological enzyme processing and corrosion-resistant Asian puccoon plant extraction liquid method, with gram
Take that alkannin content of the existing technology is low, the technological deficiency of production cycle length.
In order to achieve the above object, the present invention provides a kind of with biological enzyme processing and corrosion-resistant Asian puccoon plant extraction liquid
Preparation method, the preparation method include the following steps：
(1) using alkanet as raw material, the alkanet block that length is less than preset value is obtained after pretreatment；
(2) 20~50 parts of alkanet blocks made from step (1) are placed in 20~45 DEG C of soak and impregnate 15~30 points
Clock, the soak include 10~25 parts of complex enzyme formulation, 1~5 part of salts substances and 40~100 parts of water；
(3) the alkanet block by soak and after impregnating is transferred in processor for ultrasonic wave, under preset temperature at ultrasound
Reason 1~2 hour, isolated supernatant after static layering；
(4) supernatant removes impurity, the eluent of acquisition using the chromatography column pure water elution for being filled with resin extender
That is Asian puccoon plant extraction liquid.
Preferably, the complex enzyme formulation includes being 1 by weight：(0.5~2) the first enzyme preparation and second enzyme of mixing
Preparation, mixture of first enzyme preparation for one or more of cellulase, pectase and zytase are described
Second enzyme preparation is glucose oxidase and/or lysozyme.
Preferably, first enzyme preparation is the mixture of the cellulase, the pectase and the zytase,
And the weight ratio composition of the cellulase, the pectase and the zytase is 1：(0.5~2.5)：(0.25~1).
Preferably, first enzyme preparation and the weight ratio of the salts substances are：(3~5)：1.
Preferably, mixture of second enzyme preparation for the glucose oxidase and the lysozyme, and the glucose
The weight ratio of oxidizing ferment and the lysozyme is 1：(0.5~2).
Preferably, the complex enzyme formulation further includes Co-Q10.
Preferably, the salts substances are one or more of sodium chloride, potassium chloride, magnesium chloride and sodium carbonate
Mixture.
Preferably, the preset temperature in the step (3) is 20~45 DEG C.
Present invention simultaneously provides a kind of with biological enzyme processing and corrosion-resistant Asian puccoon plant extraction liquid, the Asian puccoon plant extract
Liquid is prepared using the preparation method of Asian puccoon plant extraction liquid described above.
Compared with the relevant technologies, biological enzyme processing and corrosion-resistant Asian puccoon plant extraction liquid and its preparation provided by the invention
Method has the advantages that：
First, Asian puccoon plant extraction liquid provided by the invention utilizes the peculiar technology of compound biological enzyme, on the one hand avoids use
Organic solvent improves the safety of Asian puccoon plant extraction liquid, on the other hand, Asian puccoon plant extraction liquid is prepared using enzyme process, purple
Careless recovery rate is up to 90%~98%, the content of naphthoquinones class total pigment between 22~24.5mg, concentration between 0.45~
0.49mg/mL, effective component content is high, and after tested, the Asian puccoon plant extraction liquid is mildly non-stimulated, has mosquito-proof sting, resists
Inflammation stops blooding and effectively goes acne and desalination scar the effect of property, can be widely applied to medical industry and cosmetics industry.
2nd, under the first enzyme preparation and the collective effect of salts substances, plant cell wall and cytoplasm are cracked comprehensively
Closed structure, Asian puccoon recovery rate height (90%~98%)；Second enzyme preparation can inhibit bacterial plaque to be formed, and can effectively remove bacterium colony kind
Group makes the Asian puccoon plant extraction liquid itself of extraction have antiseptic property, extends the service life of product.
3rd, Asian puccoon plant extraction liquid of the invention is soluble liquid, overcomes powdered Radix Arnebiae extract in all kinds of productions
The limitation of compliant applications in product system.
【Specific embodiment】
The technical solution in the embodiment of the present invention is clearly and completely described below, it is clear that described embodiment
Only it is the part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
All other embodiment that art personnel are obtained without making creative work belongs to the model that the present invention protects
It encloses.
The present invention provides a kind of biological enzyme processing and the preparation method of corrosion-resistant Asian puccoon plant extraction liquid, the method packet
Include following steps：
Step 1: using alkanet as raw material, the alkanet block that length is less than preset value is obtained after pretreatment；
Specifically, alkanet can be root, the Asian puccoon of lithospermum euchromum Royle Arnebia euchroma (Royle) Johnst
The root of Lithospermum erythrorhizon Sieb.et Zcc. or arnebia guttata Bunge Arnebia guttata Bunge's
The mixture of one or more of root, it is preferred to use lithospermum euchromum Royle root.
After alkanet is cleaned up, it is cut into small pieces, then reuses crusher machine of squeezing the juice, the length of broken alkanet block
Degree is less than 10mm.
15~30 are impregnated Step 2: 20~50 parts of alkanet blocks made from step 1 are placed in 20~45 DEG C of soak
Minute, the soak includes 10~25 parts of complex enzyme formulation, 1~5 part of salts substances and 40~100 parts of water；
The complex enzyme formulation includes being 1 by weight：(0.5~2) the first enzyme preparation and the second enzyme preparation of mixing,
In, mixture of first enzyme preparation for one or more of cellulase, pectase and zytase, described the
Two enzyme preparations are glucose oxidase and/or lysozyme.First enzyme preparation is used to crack plant cell wall and iuntercellular comprehensively
The closed structure of matter, improves Asian puccoon recovery rate, and the second enzyme preparation can inhibit bacterial plaque to be formed, can effectively remove bacterium colony population, make
The Asian puccoon plant extraction liquid of extraction itself has antiseptic property, extends the service life of product, and avoid adding in other preservatives
Make generation allergic phenomena.By the proportioning for limiting the first enzyme preparation and the second enzyme preparation so that using preparation method extraction
Not only active ingredient content is high for Asian puccoon plant extraction liquid, both effectiveness good, and will not generate anaphylaxis.The salts substances are chlorination
The mixture of one or more of sodium, potassium chloride, magnesium chloride and sodium carbonate.
Preferably, first enzyme preparation is the mixture of the cellulase, the pectase and the zytase,
And the weight ratio composition of the cellulase, the pectase and the zytase is 1：(0.5~2.5)：(0.25~1)；
Mixture of second enzyme preparation for the glucose oxidase and the lysozyme, and the glucose oxidase and the bacteriolyze
The weight ratio of enzyme is 1：(0.5~2).When the first enzyme preparation uses the cellulase, the pectase and the zytase
Mixture, the recovery rate of alkanet can further be improved by the compounding of three, when the second enzyme preparation use the glucose
The mixture of oxidizing ferment and the lysozyme also may be such that Asian puccoon plant extraction liquid has better anti-corrosion by the compounding of the two
Performance extends the service life of product.
Preferably, first enzyme preparation and the weight ratio of the salts substances are：(3~5)：1.By further limiting
The proportioning of first enzyme preparation and salts substances avoids salts substances from also can more fully destroying plant while influencing enzymatic activity thin
The closed structure of cell wall and cytoplasm obtains higher extracted rate.
Preferably, the complex enzyme formulation further includes Co-Q10.By adding in Co-Q10 in complex enzyme formulation, can carry
It is high its when shield is helped and is applied in product the effect of.
Preferably, the salts substances are sodium chloride.
Wherein, cellulase can make the mass degradations such as cellulose, pectin in cell wall or cytoplasm with pectase,
The compact texture of cell wall is destroyed, cell wall and cytoplasm structure is caused to generate the variations such as loose part, expansion, collapse, is subtracted
The resistance to mass tranfer that the mass transfers such as cellule wall, cytoplasm barrier spreads active ingredient from intracellular to Extraction medium, from mass transfer angle
Degree promotes effective component extraction rate to improve.
Xylan is the important component of plant hemicellulose, and xylan is complicated, the degradable ginseng for needing a variety of enzymes
With, and zytase is to participate in most important one kind in degradation enzyme system.Xylan refer to can by xylan degrading into oligosaccharide and
The general name of one group of enzyme of xylose, zytase can crush plant cell wall, improve the xylan degrading of nutrient for the degree of polymerization compared with
Low small molecule segment destroys the structure of cell wall, and the nutriment that cell wall fetters is released.
Glucose oxidase effectively fast decoupled glucan, dissolving bacterial plaque can inhibit bacterial plaque to be formed, can effectively remove bacterium colony
Population.
Lysozyme is a kind of alkaline enzyme that can be hydrolyzed and polysaccharide is sticked in pathogenic bacteria.Mainly by destroying the N- acetyl in cell wall
β-Isosorbide-5-Nitrae glycosidic bond between muramic acid and n-acetylglucosamine makes the insoluble glutinous polysaccharide of cell wall resolve into soluble glycopeptide,
Cell wall rupture content is caused to escape and make bacterolysis.Lysozyme can also with negatively charged virus protein directly in conjunction with,
Double salt is formed with DNA, RNA, apoprotein, makes virally inactivated, therefore, which has the effects that antibacterial, anti-inflammatory, antiviral.
Lysozyme is safe as preservative.Lysozyme is a kind of native protein, and property is very stable.
Co-Q10 is a kind of fat-soluble antioxidant, and the nutrition of energy human activin cell and cellular energy has and improves people
Body immunity enhances the functions such as anti-oxidant, anti-aging and enhancing human activity.Can go deep into cell simultaneously, strengthen cell newly into
On the other hand metabolic function, consolidation binding ability between Polygonum Multiflorum Thunb Extract form the reticular structure of elasticity in epidermis, repair really because
Wrinkle caused by fluid loss characteristics, and and then reach real moisture-keeping efficacy.
Sodium chloride can increase the viscosity of system；Osmolyte regulator, salting-out agents can be done, there is certain shadow to the activity of enzyme
It rings, influences the dissolution that enzyme cracks substance in the speed and plant cell of cell wall.Meanwhile sodium chloride also has the work of bactericidal antiphlogistic
With.
Potassium chloride is one kind of salts substances, white crystals or crystalline powder, and soluble easily in water and glycerine is insoluble in alcohol,
Insoluble in ether and acetone.In food service industry, it can be used as salt substitute applied to agricultural production, aquatic products, livestock products, fermentation, seasoning, tank
Head, instant food etc. make low Na prod, to reduce the excessively high harmful effect to body of sodium content；It is also used for strengthening potassium (for people
Body electrolyte use), prepare sports drink etc..
Magnesium chloride is important inorganic raw material in chemical industry, for producing the magnesium such as magnesium carbonate, magnesium hydroxide, magnesia
Product also serves as raw material of antifreezing agent etc..Food additives, protein coagulation agent, deicing salt, freezing can be done in other fields
Agent, dust-proofing agent, refractory material etc..
Sodium carbonate is a kind of white powder soluble easily in water, solution alkaline.It is widely used in medicine, papermaking, metallurgy, glass
The industry such as glass, weaving, dyestuff.
Step 3: the alkanet block by soak and after impregnating is transferred in processor for ultrasonic wave, surpass under preset temperature
Sonication 1~2 hour, isolated supernatant after static layering；Wherein, separation method can be centrifugation or filter, described pre-
If temperature is 20~45 DEG C.
Step 4: supernatant removes impurity, acquisition is washed using the chromatography column pure water elution for being filled with resin extender
De- liquid, that is, Asian puccoon plant extraction liquid.
The present invention also provides a kind of with biological enzyme processing and corrosion-resistant Asian puccoon plant extraction liquid, the Asian puccoon plant extraction liquid
It is prepared using the preparation method of Asian puccoon plant extraction liquid described above.
Asian puccoon plant extraction liquid described above can be applied in skin care item, specifically with mosquito-proof sting, anti-inflammatory hemostasis,
Effectively go acne and desalination scar and other effects.Certainly, the Asian puccoon plant extraction liquid can also other be industrial applied to medical industry etc.,
It does not limit herein.
First, using the method preparation of embodiment 1 to embodiment 10 with biological enzyme process and corrosion-resistant Asian puccoon plant extraction liquid,
It is specific as follows：
Embodiment 1
20 parts of alkanets are removed into impurity and stalk, cleans up, is cut into small pieces, use crusher machine of squeezing the juice；Add 40 parts
Water, 10 parts of complex enzyme formulations (2 parts of cellulase, 2 parts of pectase, 1 part of zytase, 2 parts of glucose oxidase, 3 parts of lysozyme)
It is placed in 20 DEG C of thermostat water baths with 1 part of sodium chloride and impregnates 15min；It is transferred in processor for ultrasonic wave after the completion of impregnating,
Ultrasound 1h at 20 DEG C；Stratification centrifuges or is obtained by filtration supernatant；Supernatant is made to pass through the chromatography layer for being filled with resin extender
Column is analysed, is washed with water to remove impurity, eluent, that is, Asian puccoon plant extraction liquid of collection.
Embodiment 2
25 parts of alkanets are removed into impurity and stalk, cleans up, is cut into small pieces, use crusher machine of squeezing the juice；Add 50 parts
Water, 14 parts of complex enzyme formulations (4 parts of cellulase, 3 parts of pectase, 1.5 parts of zytase, 3 parts of glucose oxidase, lysozymes 2.5
Part) and 2.5 parts of sodium chloride be placed in immersion 15min in 20 DEG C of thermostat water baths；Processor for ultrasonic wave is transferred to after the completion of impregnating
In, the ultrasound 1h at 20 DEG C；Stratification centrifuges or is obtained by filtration supernatant；Pass through supernatant and be filled with resin extender
Chromatography column is washed with water to remove impurity, eluent, that is, Asian puccoon plant extraction liquid of collection.
Embodiment 3
25 parts of alkanets are removed into impurity and stalk, cleans up, is cut into small pieces, use crusher machine of squeezing the juice；Add 50 parts
Water, 14 parts of complex enzyme formulations (2 parts of cellulase, 4.5 parts of pectase, 1.5 parts of zytase, 3.5 parts of glucose oxidase, bacteriolyzes
2.5 parts of enzyme) and 2.5 parts of salts substances (1 part of sodium chloride, 1.5 parts of magnesium chloride) be placed in 25 DEG C of thermostat water baths and impregnate 15min；
It is transferred in processor for ultrasonic wave after the completion of impregnating, the ultrasound 1h at 25 DEG C；Stratification centrifuges or is obtained by filtration supernatant
Liquid；Supernatant is made to pass through the chromatography column for being filled with resin extender, is washed with water to remove impurity, the eluent of collection is purple
Careless plant extraction liquid.
Embodiment 4
25 parts of alkanets are removed into impurity and stalk, cleans up, is cut into small pieces, use crusher machine of squeezing the juice；Add 50 parts
Water, 12.5 parts of complex enzyme formulations (4 parts of cellulase, 3 parts of pectase, 1 part of zytase, 3 parts of glucose oxidase, lysozymes 1.5
Part) and 2.5 parts of salts substances (1 part of sodium chloride, 1.5 parts of sodium carbonate) be placed in immersion 15min in 25 DEG C of thermostat water baths；It has impregnated
It is transferred in processor for ultrasonic wave after, the ultrasound 1h at 25 DEG C；Stratification centrifuges or is obtained by filtration supernatant；Make
Clear liquid is washed with water to remove impurity, eluent, that is, Asian puccoon plant of collection by being filled with the chromatography column of resin extender
Extracting solution.
Embodiment 5
25 parts of alkanets are removed into impurity and stalk, cleans up, is cut into small pieces, use crusher machine of squeezing the juice；Add 50 parts
Water, 14.5 parts of complex enzyme formulation are (3 parts of cellulase, 2.5 parts of pectase, 2 parts of zytase, 3.5 parts of glucose oxidase, molten
3.5 parts of bacterium enzyme) and 2.5 parts of sodium chloride be placed in 25 DEG C of thermostat water baths and impregnate 15min；Ultrasonic wave is transferred to after the completion of impregnating
In processor, ultrasonic 1.5 stratification, centrifuge or are obtained by filtration supernatant at 25 DEG C；Pass through supernatant and be filled with resin
The chromatography column of filler is washed with water to remove impurity, eluent, that is, Asian puccoon plant extraction liquid of collection.
Embodiment 6
25 parts of alkanets are removed into impurity and stalk, cleans up, is cut into small pieces, use crusher machine of squeezing the juice；Add 100 parts
Water, 12.5 parts of complex enzyme formulation are (2.5 parts of cellulase, 2 parts of pectase, 2.5 parts of zytase, 2 parts of glucose oxidase, molten
3.5 parts of bacterium enzyme) and 5 parts of sodium chloride be placed in 25 DEG C of thermostat water baths and impregnate 30min；It is transferred at ultrasonic wave after the completion of impregnating
It manages in device, ultrasound 1.5h, stratification centrifuge or are obtained by filtration supernatant at 25 DEG C；Pass through supernatant and be filled with resin
The chromatography column of filler is washed with water to remove impurity, eluent, that is, Asian puccoon plant extraction liquid of collection.
Embodiment 7
50 parts of alkanets are removed into impurity and stalk, cleans up, is cut into small pieces, use crusher machine of squeezing the juice；Add 100 parts
Water, 25 parts of complex enzyme formulation (6 parts of cellulase, 7 parts of pectase, 6.5 parts of lysozyme, 5.5 parts of Co-Q10) and 5 parts of salts
Substance (2.5 parts of sodium chloride, 2.5 parts of potassium chloride), which is placed in 45 DEG C of thermostat water baths, impregnates 30min；It is transferred to after the completion of impregnating
In processor for ultrasonic wave, ultrasound 2h, stratification centrifuge or are obtained by filtration supernatant at 45 DEG C；Supernatant is made to pass through filling
There is the chromatography column of resin extender, be washed with water to remove impurity, eluent, that is, Asian puccoon plant extraction liquid of collection.
Embodiment 8
50 parts of alkanets are removed into impurity and stalk, cleans up, is cut into small pieces, use crusher machine of squeezing the juice；Add 100 parts
Water, 25 parts of complex enzyme formulation (6.5 parts of cellulase, 6.5 parts of glucose oxidase, 6 parts of lysozyme, 6 parts of Co-Q10) and 5 parts
Sodium chloride, which is placed in 40 DEG C of thermostat water baths, impregnates 30min；It is transferred in processor for ultrasonic wave after the completion of impregnating, at 40 DEG C
Ultrasonic 2h, stratification centrifuge or are obtained by filtration supernatant；Supernatant is made to pass through the chromatography column for being filled with resin extender,
It is washed with water to remove impurity, eluent, that is, Asian puccoon plant extraction liquid of collection.
Embodiment 9
30 parts of alkanets are removed into impurity and stalk, cleans up, is cut into small pieces, use crusher machine of squeezing the juice；Add 60 parts
Water, 15 parts of complex enzyme formulation (2 parts of pectase, 4 parts of zytase, 5 parts of glucose oxidase, 4 parts of Co-Q10) and 3 parts of chlorinations
Sodium, which is placed in 40 DEG C of thermostat water baths, impregnates 25min；It is transferred in processor for ultrasonic wave after the completion of impregnating, it is ultrasonic at 40 DEG C
1h, stratification centrifuge or are obtained by filtration supernatant；Supernatant is made to pass through the chromatography column for being filled with resin extender, uses water
It washs to remove impurity, eluent, that is, Asian puccoon plant extraction liquid of collection.
Embodiment 10
30 parts of alkanets are removed into impurity and stalk, cleans up, is cut into small pieces, use crusher machine of squeezing the juice；Add 60 parts
Water, 15 parts of complex enzyme formulation (3.5 parts of cellulase, 2.5 parts of pectase, 4.5 parts of lysozyme, 4.5 parts of Co-Q10) and 3 parts
Salts substances (1.5 parts of sodium chloride, 1.5 parts of potassium chloride), which are placed in 25 DEG C of thermostat water baths, impregnates 25min；Turn again after the completion of impregnating
It moves in processor for ultrasonic wave, ultrasound 1h, stratification centrifuge or are obtained by filtration supernatant at 25 DEG C；Pass through supernatant
The chromatography column of resin extender is filled with, is washed with water to remove impurity, eluent, that is, Asian puccoon plant extraction liquid of collection.
2nd, embodiment provides in order to further illustrate the present invention the safety of Asian puccoon plant extraction liquid and both effectiveness, with
The advantageous effect for the Asian puccoon plant extraction liquid that embodiment provides is further illustrated the present invention by test example down：
The reference substance that experiment five is provided to experiment nine is that commercially available shikonin extract is specific as follows：
1 corresponding shikonin extract of reference substance, supplier are：Guangzhou Mei Yi bio tech ltd；
2 corresponding shikonin extract of reference substance, supplier are：Xi'an Rui Di bio tech ltd；
3 corresponding shikonin extract of reference substance, supplier are：One hundred Zhen Tang bio tech ltd of Ganzhou；
4 corresponding shikonin extract of reference substance, supplier are：Guangzhou Yu Xuan bio tech ltd.
Experiment one：Recovery rate measures (assay of naphthoquinones class total pigment)
Containing abundant naphthalene quinones substance in alkanet, by measuring Radix Arnebiae extract obtained in Examples 1 to 10
Naphthoquinones class content of material, so as to find out recovery rate.
Object on the basis of selection Acetylshikonin, a length of 530nm of maximum absorption wave of chloroformic solution.
Standard curve：Essence claims Acetylshikonin 2.5mg, is placed in 25mL colorimetric cylinders, and chlorination, which is imitated, dissolves and be diluted to scale,
Respectively draw 1.0,2.0,3.0,4.0,5.0mL, be diluted to 25mL with chloroform, using chloroform as blank, on spectrophotometer in
Absorption value is measured at 530nm, with a concentration of abscissa of Acetylshikonin, absorption value A is ordinate, prepares standard curve, to inhale
Shading value maps to standard solution (mg/mL).
The measure of naphthoquinones class total pigment in extracting solution：3 25mL colorimetric cylinders are taken, respectively as blank, sample and sample school
Just.Accurate 0,2.0mL, 2.0mL sample liquid of drawing is in three test tubes successively, and respectively plus chloroformic solution is settled to graduation mark, with light splitting
Photometer measures absorbance value A under 530nm wavelength, and the content of naphthoquinones class total pigment in extracting solution is calculated from working curve.
Test standard curve regression equation is：Y=9.668X+0.0023, related coefficient R=0.9991；Wherein X is
Rutin standard concentration (mg/mL), Y are absorbance value.
Naphthoquinones class total pigment/Asian puccoon used weight in Asian puccoon recovery rate=100% × extracting solution
Table 1
The active ingredient of Chinese medicine generally requires to abolish the obstruction of cell wall during by separation, but cell wall with
And hemicellulose, pectic substance, cellulose in cytoplasm etc. can be formed it is comparatively dense as a result, under regular situation, effectively into
It is difficult quickly dissolution to divide, and leads to extraction difficulty, using enzyme process, can increase the speed of biological respinse, plant compact tissue is led to
It crosses fast reaction and loses efficiency, active ingredient is dissolved out.By enzyme process can be applied to Asian puccoon extraction, using cellulase,
The catalytic action of zytase and pectase cracks substance in the speed and plant cell of cell wall along with salt ion influences enzyme
Dissolution, dissolve out active ingredient, recovery rate be greatly improved.The Asian puccoon recovery rate of the embodiment of the present invention 1~10 is 90%
More than.
Experiment two：Preservative efficacy testing
1st, test specimen：The Asian puccoon plant extraction liquid sample that the embodiment of the present invention 1~10 provides；Experimental bacteria is Escherichia coli
ATCC 25922, staphylococcus aureus ATCC 6538, pseudomonas aeruginosa ATCC 9027, Candida albicans ATCC
10231。
2nd, test method：Microbiology challenge test method.
3rd, result evaluation：Using《European Pharmacopoeia》Result evaluation standard this result of the test is evaluated, have
Body refers to the following table 2.
Table 2
From the test result of table 2 it is found that 4 kinds of experimental bacterias (1 week) total plate count when the 7th day declines 100%, until the 28th
It when without growth, judgement is by challenge experiment, and inhibitor effectiveness is good, i.e., Asian puccoon plant extraction liquid provided in an embodiment of the present invention
The anti-corrosion effect of protective system built using lysozyme and/or glucose oxidase is good.
Experiment three：Ocular irritation/erosion test
1st, test specimen：The Asian puccoon plant extraction liquid that the embodiment of the present invention 1~10 provides.
2nd, test method：SN/T 2329-2009 cosmetics eye irritation/corrosive chick chorioallantoic membrane experiment.
3rd, evaluation of test result standard：Stimulation standards of grading are divided into level below：IS ＜ 1 are nonirritant；1≤IS ＜ 5
For subexcite；5≤IS ＜ 9 are moderate irritation；IS >=10 are strong and stimulating/corrosivity.
4th, result of the test：The stimulation scoring of the Asian puccoon plant extraction liquid provided through overtesting, 1-10 of the embodiment of the present invention is equal
For IS ＜ 1, i.e., Asian puccoon plant extraction liquid provided in an embodiment of the present invention is mildly nonirritant to eye.
Experiment four：Skin irritation test
1st, skin closure type patch test
1) test specimen：The Asian puccoon plant extraction liquid that the embodiment of the present invention 1~10 provides.
2) test method：From a certain 22 volunteers of regional choice, the age of volunteer, body was good between 20~45 years old
Health, in the recent period non-drug administration, nonbreast-fed women or gravid woman, non-constitution sensitive subjects.Volunteer is divided into 11 groups at random,
Every group of two people.Wherein, group 1 is blank control group, and the small interior of spot examination device does not place any substance, spot is tried the low sensitization glue of device
Band is pasted on the back of volunteer or forearm bends side, is gently pressed with palm and is allowed to equably stick on the skin；Group 2~groups 11 with
The processing method of group 1 is identical, unlike：The Asian puccoon plant provided added with the 0.025mL embodiment of the present invention 1~10 is carried respectively
Liquid is taken to be put into the small interior of spot examination device；It is Asian puccoon plant extraction liquid that the embodiment of the present invention 1 provides that i.e. group 2 is corresponding, and group is 3 corresponding
It is the Asian puccoon plant extraction liquid that the embodiment of the present invention 2 provides, corresponding group 4 is that the Asian puccoon plant that the embodiment of the present invention 3 provides carries
Take liquid ..., it is Asian puccoon plant extraction liquid that the embodiment of the present invention 10 provides that group 11 is corresponding.Each group test duration
After 24 hours, 48 hours, 30min after removal spot examination device, after impression disappearance, observation sticks the response situation and note of place's skin
Record.
Wherein, skin closure type patch test dermoreaction grade scale see the table below 3.
Table 3
During entire experiment, the subject's of group 1- groups 11 is reacted negative by gesture area skin.Thus
As can be seen that Asian puccoon plant extraction liquid provided in an embodiment of the present invention will not cause the allergy and stimulate the reaction of skin, safety
It is good.
2nd, repeatability is opening smears experiment
1) test specimen：The Asian puccoon plant extraction liquid that the embodiment of the present invention 1~10 provides.
2) from a certain region select 22 volunteers, the age of volunteer between 20-45 Sui, health, in the recent period
Non- drug administration, nonbreast-fed women or gravid woman, non-constitution sensitive subjects.Volunteer is divided into 11 groups, every group two at random
It is personal.Wherein, group 1 is blank control group, is bent using forearm as recipient site, 3 × 3cm2 of area, and recipient site keeps drying,
It avoids contact with before tested and during by gesture other external preparations, with ultra-pure water 0.05 ± 0.005g/ times, equably smears tested portion
Position；Group 2~groups 11 are respectively test group, unlike group 1：The Asian puccoon plant provided using the embodiment of the present invention 1~10 is carried
Liquid is taken equably to smear recipient site 0.05 ± 0.005g/ times；Wherein, group 2 corresponds to the Asian puccoon plant that the embodiment of the present invention 1 provides
Extracting solution, group 3 correspond to the Asian puccoon plant extraction liquid that the embodiment of the present invention 2 provides, and group 4 corresponds to the purple that the embodiment of the present invention 3 provides
Careless plant extraction liquid ... and so on, group 11 corresponds to the Asian puccoon plant extraction liquid that the embodiment of the present invention 10 provides.Each group is daily
It carries out 2 times and equably smears recipient site, long run test completes experiment, observes the skin of the recipient site of subject daily after 7 days
Skin response situation simultaneously records.It, can during if there is 3 points or more in such as the following table 8 of dermoreaction situation during experiment
Stopping continues to test and record result.
Wherein, table 4 tests dermoreaction judgment criteria table for opening smear of skin repeatability.
Table 4
During entire experiment, the dermoreaction by gesture position of the subject of group 1~groups 11 is negative.By
This, which can be seen that Asian puccoon plant extraction liquid provided in an embodiment of the present invention, will not cause the allergy and stimulate the reaction of skin, safety
Property is good.
Experiment five：Biocidal property is tested
1st, test specimen：Choose Radix Arnebiae extract that commercially available reference substance 1 to 4 and 1-10 of the embodiment of the present invention provide into
Row test.
2nd, test method：With reference to the Ministry of Public Health《Disinfection technology standard》2008 editions.Test strain：It is staphylococcus aureus, big
Enterobacteria, Pseudomonas aeruginosa, Candida albicans.
During experiment, the Radix Arnebiae extract sample that the embodiment of the present invention 1~10 provides is configured to respectively a concentration of
1% sample, and be positioned in the volumetric flask or conical flask of number 1~10, it is spare.And preparation experiment bacteria suspension, average mark
It is attached in the conical flask of number 1a~10a, then, each test liquid of equivalent is taken to be added in the conical flask of 1a~10a respectively, it will
It is sufficiently mixed uniformly and after the 20min that interacts with the test strain in bacteria suspension after test liquid and experiment, starts to test and remember
Record the killing rate of the test strain in each conical flask.
3rd, result of the test：Result of the test refers to the following table 5, wherein, killing rate=(the strain quantity survived after experiment/initial
The quantity of strain) × 100%.
Table 5
From the result of the test of table 5 as can be seen that the present invention in Examples 1 to 10 provide for Staphylococcus aureus
Bacterium, Escherichia coli, Pseudomonas aeruginosa, Candida albicans killing rate more than 90%, hence it is evident that better than the sterilization of reference substance 1~4
Rate.Under the compound action of lysozyme and oenoxydase, bacterial plaque can be inhibited to be formed, bacterium colony population can be effectively removed.It is compound
Under enzyme preparation (lysozyme and oenoxydase) and the collective effect of alkannin so that the Asian puccoon plant extraction liquid of invention is effectively strong
Change bacteria resistance function.
Experiment six：Inoxidizability is tested
1st, test specimen：Commercially available reference substance 1 to 4 and the Radix Arnebiae extract of the offer of the embodiment of the present invention 1~10 are provided
It is tested.
2nd, test method：Liquor potassic permanganate titration：Prepare the liquor potassic permanganate of 5mmol/L.
The Asian puccoon plant extraction liquid of 2mL Examples 1 to 10 is taken to add in the distilled water of 50mL, so in clean conical flask
It is demarcated afterwards with the liquor potassic permanganate of 5mmol/L, when the solution in conical flask dark brown becomes lavender, and in 30s from shallow
Colour-fast, as titration end-point, record consume the volume V of potassium permanganate.
3rd, experimental result：It is as shown in Table 6 below
Table 6
| Embodiment | Consume the volume V/mL of potassium permanganate | Embodiment | Consume the volume V/mL of potassium permanganate |
| Embodiment 1 | 21.88 | Embodiment 8 | 23.48 |
| Embodiment 2 | 21.10 | Embodiment 9 | 21.13 |
| Embodiment 3 | 22.98 | Embodiment 10 | 20.03 |
| Embodiment 4 | 23.08 | Reference substance 1 | 12.35 |
| Embodiment 5 | 21.95 | Reference substance 2 | 10.30 |
| Embodiment 6 | 23.25 | Reference substance 3 | 9.85 |
| Embodiment 7 | 22.88 | Reference substance 4 | 8.66 |
With liquor potassic permanganate redox reaction, the volume of the potassium permanganate of consumption can occur for Asian puccoon plant extraction liquid
It is more, illustrate that, containing more reducing substances in extracting solution, the inoxidizability that represent the extracting solution is stronger.As known from Table 6,
The inoxidizability ability of Asian puccoon plant extraction liquid is better than commercially available reference substance 1~4, anti-oxidant energy in the embodiment of the present invention 1~10
Power is strong.
Experiment seven：Mosquito-proof sting experiment
The fresh pig blood of 50mL will be packed into clean beaker, then adds in the distilled water of 50mL as blank control, compile
Number 1；The fresh pig blood of 50mL will be packed into clean beaker, then adds in the Asian puccoon plant extraction liquid in the embodiment 1 of 50mL,
Number 2；The fresh pig blood of 50mL will be packed into clean beaker, then adds in the Asian puccoon plant extract in the embodiment 2 of 50mL
Liquid, number 3 ... and so on, number 11 corresponds to the Asian puccoon plant extraction liquid that the embodiment of the present invention 10 provides；Number 12 corresponds to
The Asian puccoon plant extraction liquid of reference substance 1；Number 13 corresponds to the Asian puccoon plant extraction liquid of reference substance 2；Number 14 corresponds to reference substance 3
Asian puccoon plant extraction liquid；Number 15 corresponds to the Asian puccoon plant extraction liquid of reference substance 4.
The number beaker of 1~No. 15 is covered into upper big cloche with holes successively, pours into 10 mosquitoes successively from glass orifice,
Glass orifice is sealed, observes the active state of mosquito in beaker in 0~5min.
Mosquito repellent rate=(mosquito populations/mosquito total quantity in mosquito total quantity-blood solution) × 100%.
Table 7
By the experimental result of table 7 it is found that Asian puccoon plant extraction liquid repelling effect in the embodiment of the present invention 1~10
Good, mosquito repellent rate is up to 100%, can effectively mosquito-proof sting；And the Asian puccoon plant extraction liquid of commercially available competing product 1~4 is for mosquito
Highest mosquito repellent rate is only 50%.
Experiment eight：Haemostatic effect is tested
Healthy adult hero rabbit 15 is taken, and size is identical, the male rabbit blood of equivalent is extracted with syringe, take out syringe,
Blood outlet is pressed with Asian puccoon plant extraction liquid, the cotton of reference substance 1~4 in the clear water containing 2mL, Examples 1 to 10 successively,
The time required to record hemostasis completely.
Table 8
By the experimental result of table 8 it is found that Asian puccoon plant extraction liquid in the embodiment of the present invention 1~10 energy in 30s
Hemostasis, bleeding stopping period are substantially better than reference substance 1~4 completely, and embodying the Asian puccoon plant extraction liquid of the present invention has preferable hemostasis
Effect.
Experiment nine：Go acne and desalination scar effect
1st, test specimen：Asian puccoon plant in Asian puccoon plant extraction liquid and reference substance 1~4 that the embodiment of the present invention 6 provides
Extracting solution.
2nd, acne effect is gone：From a certain region select 50 volunteers, the age of volunteer between 20-45 Sui, body
Health, in the recent period non-drug administration, nonbreast-fed women or gravid woman, non-constitution sensitive subjects, face is with small pox.It at random will aspiration
Person is divided into 5 groups, every group of 10 people.Asian puccoon plant extraction liquid is applied at scar, once in the morning and once at night, twice a day, three
The Disappearance Scenarios of facial small pox are observed after month.
3rd, desalinate scar effect：From a certain region select 50 volunteers, the age of volunteer between 20-45 Sui,
Health, there are apparent scar in non-drug administration, nonbreast-fed women or gravid woman, non-constitution sensitive subjects, face in the recent period.At random
Volunteer is divided into 5 groups, every group of 10 people.Asian puccoon plant extraction liquid is applied at scar, once in the morning and once at night, one day two
It is secondary, the desalination situation at its scar is observed after three months.
By volunteer using Contrast on effect facial before and after Asian puccoon plant extraction liquid, number is collected using questionnaire form
According to acne effect and desalination scar effect is gone to be subject to volunteer's feedback effects data：
Wherein remove acne rate=100% × number (go acne effect assessment good)/total number of persons
Desalinate scar rate=100% × number (desalination scar effect assessment is good)/total number of persons
Table 9
| Embodiment 6 | Reference substance 1 | Reference substance 2 | Reference substance 3 | Reference substance 4 | |
| Number (goes acne effect to comment) | 23 | 8 | 6 | 7 | 5 |
| Go acne rate | 92% | 32% | 24% | 28% | 20% |
| Desalinate scar rate | 21 | 10 | 9 | 8 | 9 |
| Number (desalination scar effect is commented) | 84% | 40% | 36% | 32% | 36% |
Volunteer does not occur adverse reaction (such as facial redness, allergy etc.), the present invention during three months experience
The Asian puccoon plant extraction liquid of middle embodiment 6 is to be substantially better than reference substance 1~4 for going acne effect and desalination scar effect, goes acne
Rate and desalination scar rate be respectively 92%, 84%, and reference substance 1~4 go acne rate and desalination scar be much smaller than 50%.
Biological enzyme processing and corrosion-resistant Asian puccoon plant extraction liquid and preparation method thereof provided by the invention have with following
Beneficial effect：
First, Asian puccoon plant extraction liquid provided by the invention utilizes the peculiar technology of compound biological enzyme, on the one hand avoids use
Organic solvent improves the safety of Asian puccoon plant extraction liquid, on the other hand, Asian puccoon plant extraction liquid is prepared using enzyme process, purple
Careless recovery rate is up to 90%~98%, the content of naphthoquinones class total pigment between 22~24.5mg, concentration between 0.45~
0.49mg/mL, effective component content is high, and after tested, the Asian puccoon plant extraction liquid is mildly non-stimulated, has mosquito-proof sting, resists
Inflammation stops blooding and effectively goes acne and desalination scar the effect of property, can be widely applied to medical industry and cosmetics industry.
2nd, under the first enzyme preparation and the collective effect of salts substances, plant cell wall and cytoplasm are cracked comprehensively
Closed structure, Asian puccoon recovery rate height (90%~98%)；Second enzyme preparation can inhibit bacterial plaque to be formed, and can effectively remove bacterium colony kind
Group makes the Asian puccoon plant extraction liquid itself of extraction have antiseptic property, extends the service life of product.
3rd, Asian puccoon plant extraction liquid of the invention is soluble liquid, overcomes powdered Radix Arnebiae extract in all kinds of productions
The limitation of compliant applications in product system.
Above-described is only embodiments of the present invention, it should be noted here that for those of ordinary skill in the art
For, without departing from the concept of the premise of the invention, improvement can also be made, but these belong to the protection model of the present invention
It encloses.
Claims (9)
1. a kind of biological enzyme processing and the preparation method of corrosion-resistant Asian puccoon plant extraction liquid, which is characterized in that including walking as follows
Suddenly：
(1) using alkanet as raw material, the alkanet block that length is less than preset value is obtained after pretreatment；
(2) 20~50 parts of alkanet blocks made from step (1) are placed in 20~45 DEG C of soak and impregnated 15~30 minutes, institute
State complex enzyme formulation, 1~5 part of salts substances and 40~100 parts of water that soak includes 10~25 parts；
(3) by soak and impregnate after alkanet block be transferred in processor for ultrasonic wave, under preset temperature be ultrasonically treated 1~
2 hours, isolated supernatant after static layering；
(4) supernatant removes impurity, the eluent of acquisition is purple using the chromatography column pure water elution for being filled with resin extender
Careless plant extraction liquid.
2. biological enzyme processing and the preparation method of corrosion-resistant Asian puccoon plant extraction liquid, feature according to claim 1
It is, the complex enzyme formulation includes being 1 by weight：(0.5~2) the first enzyme preparation and the second enzyme preparation of mixing, it is described
Mixture of first enzyme preparation for one or more of cellulase, pectase and zytase, the second enzyme system
Agent is glucose oxidase and/or lysozyme.
3. biological enzyme processing and the preparation method of corrosion-resistant Asian puccoon plant extraction liquid, feature according to claim 2
It is, mixture of first enzyme preparation for the cellulase, the pectase and the zytase, and the fiber
The weight ratio composition of plain enzyme, the pectase and the zytase is 1：(0.5~2.5)：(0.25~1).
4. biological enzyme processing and the preparation method of corrosion-resistant Asian puccoon plant extraction liquid, feature according to claim 2
It is, the weight ratio of first enzyme preparation and the salts substances is：(3~5)：1.
5. biological enzyme processing and the preparation method of corrosion-resistant Asian puccoon plant extraction liquid, feature according to claim 2
It is, mixture of second enzyme preparation for the glucose oxidase and the lysozyme, and the glucose oxidase and institute
The weight ratio for stating lysozyme is 1：(0.5~2).
6. according to claim 2 to 5 any one of them biological enzyme processing and the preparation side of corrosion-resistant Asian puccoon plant extraction liquid
Method, which is characterized in that the complex enzyme formulation further includes Co-Q10.
7. biological enzyme processing and the preparation method of corrosion-resistant Asian puccoon plant extraction liquid, feature according to claim 1
It is, mixture of the salts substances for one or more of sodium chloride, potassium chloride, magnesium chloride and sodium carbonate.
8. biological enzyme processing and the preparation method of corrosion-resistant Asian puccoon plant extraction liquid, feature according to claim 1
It is, the preset temperature in the step (3) is 20~45 DEG C.
9. a kind of biological enzyme processing and corrosion-resistant Asian puccoon plant extraction liquid, which is characterized in that the Asian puccoon plant extraction liquid is adopted
It is prepared with the preparation method of claim 1 to 8 any one of them Asian puccoon plant extraction liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810296325.7A CN108210542A (en) | 2018-03-30 | 2018-03-30 | A kind of biological enzyme processing and corrosion-resistant Asian puccoon plant extraction liquid and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810296325.7A CN108210542A (en) | 2018-03-30 | 2018-03-30 | A kind of biological enzyme processing and corrosion-resistant Asian puccoon plant extraction liquid and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108210542A true CN108210542A (en) | 2018-06-29 |
Family
ID=62657408
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810296325.7A Pending CN108210542A (en) | 2018-03-30 | 2018-03-30 | A kind of biological enzyme processing and corrosion-resistant Asian puccoon plant extraction liquid and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108210542A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114540225A (en) * | 2022-02-18 | 2022-05-27 | 南京合谷生命生物科技有限公司 | Alkannin root endophyte and screening method, culture method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105949045A (en) * | 2016-06-28 | 2016-09-21 | 郭迎庆 | Method for extracting alkanin from arnebia roots |
| CN106106991A (en) * | 2016-07-02 | 2016-11-16 | 汪凌峰 | A kind of Radix Arnebiae (Radix Lithospermi) local flavor eggfruit hard sugar and production method |
-
2018
- 2018-03-30 CN CN201810296325.7A patent/CN108210542A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105949045A (en) * | 2016-06-28 | 2016-09-21 | 郭迎庆 | Method for extracting alkanin from arnebia roots |
| CN106106991A (en) * | 2016-07-02 | 2016-11-16 | 汪凌峰 | A kind of Radix Arnebiae (Radix Lithospermi) local flavor eggfruit hard sugar and production method |
Non-Patent Citations (1)
| Title |
|---|
| 王鑫国 主编: "《中药药理学实验教程》", 30 November 2010, 中国中医药出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114540225A (en) * | 2022-02-18 | 2022-05-27 | 南京合谷生命生物科技有限公司 | Alkannin root endophyte and screening method, culture method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103976907B (en) | A kind of cosmetics comprising the compositionss with antiallergic effect of releiving, its preparation method and application | |
| CN110464691B (en) | Peach gum composition and application thereof | |
| CN107616956A (en) | A kind of aloe skin care compositions and preparation method thereof | |
| CN103749571B (en) | A kind of attapulgite sterilization mite suppressor and preparation method thereof | |
| CN103505490B (en) | A kind of cleaning wound liquid | |
| CN101647768B (en) | Process for preparing Chinese herbal medicine toothpaste containing dragon's blood extract | |
| CN110464682A (en) | A kind of peach gum composition, preparation method and application | |
| CN107519081A (en) | A kind of composition and preparation method that effect is repaired with anti-acne | |
| CN106177339A (en) | A kind of method preparing treatment gout herbal formulation | |
| CN107184427A (en) | A kind of cooperative compositions and application containing polyglutamic acid and hyaluronic acid | |
| CN110029026A (en) | A kind of sterilizing and itch-relieving medicated soap and preparation method thereof | |
| CN110327246A (en) | A kind of mouthwash containing marine oligosaccharide | |
| CN109316419B (en) | Plant composition fermentation raw stock for balancing skin micro-ecology and preparation and application thereof | |
| CN109568217A (en) | A kind of preparation method of Salvia root P.E and its application in anti-acne | |
| CN108210542A (en) | A kind of biological enzyme processing and corrosion-resistant Asian puccoon plant extraction liquid and preparation method thereof | |
| CN108635300A (en) | A kind of cosmetics containing small molecule dendrobium polysaccharide and preparation method and application | |
| CN108420758A (en) | A kind of biological enzyme processing and corrosion-resistant honeysuckle flowers plant extraction liquid and preparation method thereof | |
| CN105193686B (en) | A kind of Feminine care solution and preparation method thereof | |
| CN106668331A (en) | Composition having functions of diminishing inflammation, inhibiting bacteria and promoting wound healing and preparation method thereof | |
| CN108272683A (en) | A kind of biological enzyme processing and corrosion-resistant moisturizing plant extraction liquid and the preparation method and application thereof | |
| Shams et al. | In vitro antidiabetic, anti-inflammatory and antioxidant potential of the ethanol extract of Uromastyx hardwickii skin | |
| CN103536622A (en) | Application of sargassum fusiforme sulphated polysaccharide in preparation of anti-aging drugs and healthcare products | |
| CN103372076A (en) | Medicine for treating diseases caused by propionibacterium acnes and application of sanguisorba officinalis and/or sanguisorba officinalis extract | |
| CN109288884A (en) | A kind of preparation method and application of the Helianthi extract with repair of releiving | |
| CN108524349A (en) | A kind of biological enzyme processing and corrosion-resistant chamomile plant extraction liquid and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180629 |
|
| RJ01 | Rejection of invention patent application after publication |