CN108207812A - A kind of preparation method of vermiculture base-material - Google Patents

A kind of preparation method of vermiculture base-material Download PDF

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Publication number
CN108207812A
CN108207812A CN201711370882.0A CN201711370882A CN108207812A CN 108207812 A CN108207812 A CN 108207812A CN 201711370882 A CN201711370882 A CN 201711370882A CN 108207812 A CN108207812 A CN 108207812A
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CN
China
Prior art keywords
base
bacillus
vegetables
temperature
stalk
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
CN201711370882.0A
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Chinese (zh)
Inventor
汤瑶
王红
黄磊
陈昱
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Austria (tianjin) Environmental Protection Technology Co Ltd
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Austria (tianjin) Environmental Protection Technology Co Ltd
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Priority to CN201711370882.0A priority Critical patent/CN108207812A/en
Publication of CN108207812A publication Critical patent/CN108207812A/en
Withdrawn legal-status Critical Current

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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • A23K10/26Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F3/00Fertilisers from human or animal excrements, e.g. manure
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

Abstract

The present invention relates to a kind of preparation methods of vermiculture base-material, belong to biomass circulating and utilize field.The vermiculture base-material of the present invention is made of cow dung 35%, agricultural crop straw 25%, vegetables 40%, the whole hot fermentation in 40 biochemical reactors of TBH CSCL, fermentation condition is 20 rpm of rotating speed of agitator, 55 DEG C of temperature, material water ratio is between 55%~60% in appropriate moisturizing maintenance reactor, it treats that temperature is stable at 55 DEG C, microbial inoculum A is added in after half an hour;Stop heating after 80h and be cooled to room temperature, air blow and agitation is always maintained in reactor, until material spontaneously dries.The material of earthworm base-material of the present invention derives from a wealth of sources, and is formulated degree of freedom higher, and nutrient is more balanced, and ferment whole high temperature, and base-material is more stable after fermentation.

Description

A kind of preparation method of vermiculture base-material
Technical field
The present invention relates to a kind of preparation methods of vermiculture base-material, belong to biomass circulating and utilize field.
Background technology
The artificial earliest country of vermiculture is the U.S. and New Zealand, and cultivating larger country at present has the U.S., Australia Big Leah, Canada, Japan, New Zealand etc., especially the U.S. has evolved to industrialized aquaculture and merchandized handling stage.Manually Vermiculture raw material be mainly garden waste, house refuse, manure of livestock and poultry, sludge, food factory or fiber process factory or paper mill Deng production waste;Vermiculture product is mainly wormcast and vermis, and wormcast and its deep processed product can be used as organic fertilizer Material is sold, vermis and its and its deep processed product can be used for the fields such as drug extraction, animal protein feed, environmental protection.Earthworm Excrement and vermis have been demonstrated have considerable economic value, but since the artificial vermiculture in China is started late, technology, Industry etc. has the potentiality of very big room for promotion, and vermiculture is entirely possible to be developed to as an emerging environmental protection industry Chain.
China's vermiculture industry develops by decades, and cultural technique also reaches its maturity and becomes more meticulous.Earthworm base-material is Vermiculture important foundation, different vermiculture products in order to obtain, material composition is also what is changed therewith in base-material, is especially made For medicine and edible earthworm, it is necessary to the source of material in stringent control base-material.At present wormcast organic fertilizer market relative maturity and Demand is larger, and base material formulation ingredient mainly includes manure of livestock and poultry(Pigs and cattle sheep manure, chicken and duck goose excrement), agricultural crop straw or gardens discard Object, gourd, fruit and vegetable.Wormcast organic fertilizer base-material manufacturing process there are the problem of be:1)Each component ratio is unreasonable in formula, matches It is unfavorable for vermiculture than base-material out;2)Base-material fermentation is generally using Traditional compost method, and time-consuming too long, fermentation is not thorough enough Bottom;3)Base-material fabrication cycle is longer, can not steady and continuous supply, constrain further industrialized development.
To solve the above problems, the present invention has done material ratio in wormcast organic fertilizer base-material intense adjustment, object is controlled Expect source, ensure the quality of organic fertilizer;Complete to ferment by TBH-CSCL-40 biochemical reactors simultaneously, make fermentation process it is stable, Efficiently.
Invention content
To overcome the shortcomings of wormcast organic fertilizer base-material manufacturing process in background technology, the present invention relates to a kind of vermicultures The preparation method of base-material, in the method material because ground source of drawing material it is extensive, formula rate is reasonable, and nutrient is more balanced, fermentation Whole high temperature, the base-material after fermentation are more stable.
Technical scheme of the present invention includes:(1)By seven plants of bacterium Bacillus sp. MN-07, Bacillus Coagulans, Bacillus coagulans strain KM-1, Bacillus coagulans 36D1, Bacillus Licheniformis strain Pb-WC09009, Bacillus tequilensis strain CGX5-1, Bacillus Licheniformis strain TS-11 are inoculated in 500 mL LB culture mediums and are cultivated 24 hours under the conditions of 55 DEG C respectively. Single bacterium liquid is mixed into microbial inoculum A according to equal proportion.Wherein LB culture mediums:10 g of peptone, 5 g of dusty yeast, 5 g of sodium chloride, PH value 7.4,1 L of distilled water;(2)By agricultural crop straw, grates vegetables to 1~10 mm, earthworm base-material is according to cow dung 35%, farming The mass ratio mixing of object stalk 25%, vegetables 40% is placed in model TBH-CSCL-40 biochemical reactors, sets rotating speed of agitator 20 rpm, 55 DEG C of temperature, appropriate moisturizing maintain in reactor material water ratio to treat that temperature is stable at 55 DEG C between 55%~60%, Microbial inoculum A is added in after half an hour;Stop heating after 80h and be cooled to room temperature, air blow and agitation is always maintained in reaction, until material is certainly It is so dry;
Beneficial effects of the present invention:1)In earthworm base ingredients agricultural crop straw and vegetables because ground source of drawing material it is extensive, it is at low cost It is honest and clean, 2)Fermentation process whole process high temperature, fermenting speed are exceedingly fast, and material fermentation is abundant, and the earthworm base-material after fermentation is stablized;3)Earthworm base Material formula ratio is reasonable, and nutrient is more balanced;4)This production method is conducive to preparation earthworm base-material industrialization conversion.
Specific embodiment
Embodiment 1
Prepare LB culture mediums:10 g of peptone, 5 g of dusty yeast, 5 g of sodium chloride, pH value 7.4,1 L of distilled water;By seven plants of bacterium Bacillus sp. MN-07, Bacillus coagulans, Bacillus coagulans strain KM-1, Bacillus Coagulans 36D1, Bacillus licheniformis strain Pb-WC09009, Bacillus tequilensis Strain CGX5-1, Bacillus licheniformis strain TS-11 are inoculated in respectively in 500 mL LB culture mediums It is cultivated 24 hours under the conditions of 55 DEG C, single bacterium liquid is then mixed into microbial inoculum A according to equal proportion.
By maize straw, vegetables(Chinese cabbage, tomato, spinach, rape etc.)Be crushed to 1~10 mm, earthworm base-material according to The mass ratio mixing of cow dung 35%, agricultural crop straw 25%, vegetables 40% is placed in model TBH-CSCL-40 biochemical reactors, if 20 rpm of rotating speed of agitator, 55 DEG C of temperature are put, appropriate moisturizing maintains material water ratio in reactor between 55%~60%, to treat temperature 55 DEG C are stable at, microbial inoculum A is added in after half an hour;Stop heating after 80h and be cooled to room temperature, it is logical that reaction process is always maintained at stirring Wind, until material spontaneously dries.Earthworm base-material after fermentation is used for breeding earthworm and collects wormcast organic fertilizer.
Embodiment 2
Prepare LB culture mediums:10 g of peptone, 5 g of dusty yeast, 5 g of sodium chloride, pH value 7.4,1 L of distilled water;By seven plants of bacterium Bacillus sp. MN-07, Bacillus coagulans, Bacillus coagulans strain KM-1, Bacillus Coagulans 36D1, Bacillus licheniformis strain Pb-WC09009, Bacillus tequilensis Strain CGX5-1, Bacillus licheniformis strain TS-11 are inoculated in respectively in 500 mL LB culture mediums It is cultivated 24 hours under the conditions of 55 DEG C, single bacterium liquid is then mixed into microbial inoculum A according to equal proportion.
By rice straw, vegetables(Chinese cabbage, tomato, spinach, rape etc.)Be crushed to 1~10 mm, earthworm base-material according to The mass ratio mixing of cow dung 35%, agricultural crop straw 25%, vegetables 40% is placed in model TBH-CSCL-40 biochemical reactors, if 20 rpm of rotating speed of agitator, 55 DEG C of temperature are put, appropriate moisturizing maintains material water ratio in reactor between 55%~60%, to treat temperature 55 DEG C are stable at, microbial inoculum A is added in after half an hour;Stop heating after 80h and be cooled to room temperature, it is logical that reaction process is always maintained at stirring Wind, until material spontaneously dries.Earthworm base-material after fermentation is used for breeding earthworm and collects wormcast organic fertilizer.

Claims (2)

1. a kind of preparation method of vermiculture base-material, it is characterized in that northern earthworm base material formulation is cow dung 35%, agricultural crop straw 25%th, vegetables 40%, wherein agricultural crop straw are maize straw or wheat stalk or cotton stalk or three's any mixture;South Earthworm base material formulation is cow dung 35%, agricultural crop straw 25%, vegetables 40%, and wherein crops are rice straw, rape stalk, Huang Beanstalk stalk or three's any mixture.
2. a kind of preparation method of vermiculture base-material, it is characterized in that base-material fermentation process:1. by crops stalk crushing to 1~ 10 mm;2. by seven plants of bacterium Bacillus sp. MN-07, Bacillus coagulans, Bacillus coagulans Strain KM-1, Bacillus coagulans 36D1, Bacillus licheniformis strain Pb-WC09009, Bacillus tequilensis strain CGX5-1, Bacillus licheniformis strain TS-11 connect respectively Kind is cultivated 24 hours in 500 mL LB culture mediums under the conditions of 55 DEG C.Single bacterium liquid is mixed into microbial inoculum A according to equal proportion. Wherein LB culture medium prescriptions:10 g of peptone, 5 g of dusty yeast, 5 g of sodium chloride, pH value 7.4,1 L of distilled water;3. by crops Stalk, grates vegetables to 1~10 mm, earthworm base-material are mixed according to the mass ratio of cow dung 35%, agricultural crop straw 25%, vegetables 40% It is placed in model TBH-CSCL-40 biochemical reactors, 20 rpm of rotating speed of agitator, 55 DEG C of temperature is set, appropriate moisturizing maintains Material water ratio treats that temperature is stable at 55 DEG C, microbial inoculum A is added in after half an hour between 55%~60% in reactor;Stop adding after 80h Heat is cooled to room temperature, and air blow and agitation is always maintained in reaction, until material spontaneously dries.
CN201711370882.0A 2017-12-19 2017-12-19 A kind of preparation method of vermiculture base-material Withdrawn CN108207812A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111470890A (en) * 2020-04-07 2020-07-31 仲恺农业工程学院 Vegetable tail vegetable processing method

Citations (5)

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Publication number Priority date Publication date Assignee Title
CN101069497A (en) * 2007-06-21 2007-11-14 李金穗 Fly maggot, earthworm ecological efficient circulation culturing method and culturing apparatus therefor
CN101233835A (en) * 2007-01-30 2008-08-06 贵州黔龙生态有机农业发展有限责任公司 Method for cultivating earthworm by cattle manure and corps stalks
CN105145498A (en) * 2015-09-23 2015-12-16 界首市地龙养殖专业合作社 High-yield earthworm breeding method
CN106797918A (en) * 2017-01-18 2017-06-06 广东省农业科学院茶叶研究所 A kind of earthworm cultivation substratum and preparation method thereof
CN106938949A (en) * 2017-04-10 2017-07-11 山东米高生物科技股份有限公司 A kind of preparation method for loading high concentration bacillus subtilis kitchen garbage organic fertilizer

Patent Citations (5)

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Publication number Priority date Publication date Assignee Title
CN101233835A (en) * 2007-01-30 2008-08-06 贵州黔龙生态有机农业发展有限责任公司 Method for cultivating earthworm by cattle manure and corps stalks
CN101069497A (en) * 2007-06-21 2007-11-14 李金穗 Fly maggot, earthworm ecological efficient circulation culturing method and culturing apparatus therefor
CN105145498A (en) * 2015-09-23 2015-12-16 界首市地龙养殖专业合作社 High-yield earthworm breeding method
CN106797918A (en) * 2017-01-18 2017-06-06 广东省农业科学院茶叶研究所 A kind of earthworm cultivation substratum and preparation method thereof
CN106938949A (en) * 2017-04-10 2017-07-11 山东米高生物科技股份有限公司 A kind of preparation method for loading high concentration bacillus subtilis kitchen garbage organic fertilizer

Non-Patent Citations (1)

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尹作乾等: "牛粪、玉米秸秆青贮废弃物和蔬菜废叶不同比例养殖蚯蚓的试验探究", 《甘肃畜牧兽医》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111470890A (en) * 2020-04-07 2020-07-31 仲恺农业工程学院 Vegetable tail vegetable processing method

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Application publication date: 20180629