CN108180950B - System and method for detecting quality of biochemical reaction container - Google Patents
System and method for detecting quality of biochemical reaction container Download PDFInfo
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- CN108180950B CN108180950B CN201810256321.6A CN201810256321A CN108180950B CN 108180950 B CN108180950 B CN 108180950B CN 201810256321 A CN201810256321 A CN 201810256321A CN 108180950 B CN108180950 B CN 108180950B
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- 238000005842 biochemical reaction Methods 0.000 title claims abstract description 51
- 238000000034 method Methods 0.000 title claims abstract description 11
- 238000001514 detection method Methods 0.000 claims abstract description 20
- 230000003287 optical effect Effects 0.000 claims abstract description 14
- 230000008878 coupling Effects 0.000 claims abstract description 3
- 238000010168 coupling process Methods 0.000 claims abstract description 3
- 238000005859 coupling reaction Methods 0.000 claims abstract description 3
- 230000005540 biological transmission Effects 0.000 claims description 19
- 238000005286 illumination Methods 0.000 claims description 10
- 238000002834 transmittance Methods 0.000 claims description 7
- 230000003321 amplification Effects 0.000 claims description 5
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 5
- 239000003344 environmental pollutant Substances 0.000 claims description 4
- 231100000719 pollutant Toxicity 0.000 claims description 4
- 230000000630 rising effect Effects 0.000 claims description 4
- 238000006243 chemical reaction Methods 0.000 abstract description 12
- 230000007547 defect Effects 0.000 abstract description 2
- 238000010586 diagram Methods 0.000 description 3
- 230000000737 periodic effect Effects 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01D—MEASURING NOT SPECIALLY ADAPTED FOR A SPECIFIC VARIABLE; ARRANGEMENTS FOR MEASURING TWO OR MORE VARIABLES NOT COVERED IN A SINGLE OTHER SUBCLASS; TARIFF METERING APPARATUS; MEASURING OR TESTING NOT OTHERWISE PROVIDED FOR
- G01D21/00—Measuring or testing not otherwise provided for
- G01D21/02—Measuring two or more variables by means not covered by a single other subclass
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- General Physics & Mathematics (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
A quality detection system and method for detecting biochemical reaction container, the system includes: a signal acquisition system that is used for giving square wave cycle rotation signal's when the code wheel rotates serial number opto-coupler, code wheel, with code wheel coaxial coupling's colorimetric disk, the biochemical reaction container that awaits measuring and the positive contrast color disk that sets up on the colorimetric disk, wherein: the biochemical reaction container is selectively arranged on the color palette, so that the signal acquisition system acquires signals from the serial number optocoupler, the signals are selectively shielded by the biochemical reaction container, and the quality detection result of the biochemical reaction container is obtained by calculating the corresponding detected optical signals. The invention can accurately detect the reaction container with quality defects.
Description
Technical Field
The invention relates to a technology in the field of medical equipment, in particular to a method for detecting the quality of a reaction container for a full-automatic biochemical analyzer.
Background
The reaction container for the full-automatic biochemical analyzer has the advantages that the light transmittance, the parallelism and the width of an effective light transmission area of the light transmission surface of the reaction container are the same, and the pollution or scratch degree of the effective light transmission area directly influences the clinical performance data of the reagent and sample reaction of the full-automatic biochemical analyzer. In the existing method for inspecting the reaction container, only the transmittance of the reaction container is inspected, and in the actual use process, the clinical performance test result of the instrument is abnormal possibly because the light-transmitting surface of the reaction container is polluted, scratched and other factors.
Disclosure of Invention
The invention provides a system and a method for detecting the quality of a biochemical reaction container, which can accurately detect the reaction container with quality defects.
The invention is realized by the following technical scheme:
the invention relates to a quality detection system for detecting biochemical reaction containers, which comprises: a signal acquisition system that is used for giving square wave cycle rotation signal's when the code wheel rotates serial number opto-coupler, code wheel, with code wheel coaxial coupling's colorimetric disk, the biochemical reaction container that awaits measuring and the positive contrast color disk that sets up on the colorimetric disk, wherein: the biochemical reaction container is selectively arranged on the color palette, so that the signal acquisition system acquires signals from the serial number optocoupler, the signals are selectively shielded by the biochemical reaction container, and the quality detection result of the biochemical reaction container is obtained by calculating the corresponding detected optical signals.
The signal acquisition system comprises: the device comprises an illumination module, a photocell module, a pre-amplifying module, an AD amplifying module and a calculating module, wherein: the illumination module is arranged in the color comparison disc and is opposite to the photocell module positioned outside the color comparison disc, and light emitted by the illumination module irradiates the photocell module through the biochemical reaction container to be detected and the grating; the photoelectric cell module converts the light signal into a current signal, the pre-amplifying module converts the current signal output by the photoelectric cell into a voltage signal, the AD amplifying module amplifies the voltage signal and converts the voltage signal into a digital signal, and the calculating module calculates and obtains a quality detection result according to the time width, the intensity and the code wheel rotating speed of the digital signal.
Preferably, a lens and a reflecting mirror are arranged between the illumination module and the photocell module, so that the length of the light path is adjusted.
The quality detection result comprises the light transmittance of the biochemical reaction container, the width of the effective light transmission surface, the wall thickness at two sides of the light transmission surface, the parallelism of the light transmission surface, scratches on the light transmission surface and the size of pollutants.
The signal acquisition system is further provided with a change curve display storage system for drawing, displaying and deriving the light signal, and the display storage system comprises: the display module is used for displaying the delay curve acquired by the information acquisition system and the storage module is used for storing the delay curve signal acquired by the signal acquisition system.
The storage module is preferably internally provided with a quality detection threshold, and the quality detection threshold is compared with the quality detection threshold and the comparison result is output to the display module after the quality detection result is calculated by the signal acquisition system.
The invention relates to a quality detection method for detecting biochemical reaction vessels of the system, which is characterized in that the energy of different optical signals before and after the biochemical reaction vessels are placed is collected, the ratio of the energy to the light transmittance is calculated, the width of an effective light transmission surface of the biochemical reaction vessels is calculated by detecting the width of a flat stage of an optical signal scanning curve, the wall thickness of two sides of the light transmission surface of the reaction vessels is calculated by detecting the width of the rising edge and the falling edge of the signal scanning curve, the parallelism of the light transmission surface of the reaction vessels is calculated by detecting the slope of the flat stage of the signal scanning curve, the scratch or the size of pollutants of the light transmission surface of the reaction vessels is calculated by detecting the width of a pit of the signal scanning curve, and finally the quality detection result is obtained after comparing the calculation result with a threshold value.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention; wherein: the device comprises a serial number optocoupler 1, a color palette 2, a signal acquisition system 3, a biochemical reaction container 4 and a code palette 5;
FIG. 2 is a partial schematic view of the major components of the structure of the present invention; wherein: the device comprises a pre-amplifying module 6, a grating 7, an illumination module 8, an AD amplifying module 9 and a photocell module 10;
FIG. 3 is a schematic diagram of a signal scanning curve according to an embodiment;
FIG. 4 is a schematic width of the plateau of the scan curve of FIG. 3;
FIG. 5 is a graph showing the slope of the plateau of the scan curve of FIG. 3;
fig. 6 is a schematic diagram of the width of the scanning curve pit of fig. 3.
Detailed Description
As shown in fig. 1, the apparatus for testing a biochemical reaction vessel according to the present embodiment comprises: the device comprises a serial number optocoupler 1 for giving square wave periodic rotation signals of a code disc 5 during rotation, the code disc 5, a color comparison disc 2 coaxially connected with the code disc 5, a biochemical reaction container 4 to be detected arranged on the color comparison disc 2 and a signal acquisition system 3 for the positive color comparison disc 2, wherein: the biochemical reaction container 4 is selectively arranged on the color palette 2, so that the signal acquisition system 3 acquires signals from the serial number optocoupler 1, the signals are selectively shielded by the biochemical reaction container 4, and the corresponding ordinate of the corresponding signal scanning curve platform area is different, so that the ratio of the two is calculated.
The square wave periodic rotation signal comprises the rotation speed v (mm/ms) and the rotation time t (ms) of the code wheel 5.
As shown in fig. 2, the light emitted by the illumination module 8 is focused to the biochemical reaction container 4 through a lens, the light emitted by the biochemical reaction container 4 is focused to the grating 7 in the signal acquisition system through the lens, the light split by the grating 7 irradiates the photocell module 10, the photocell module 10 converts an optical signal into a current signal, the pre-amplification module 6 amplifies and converts the current signal converted by the photocell module 10 into a voltage signal, and the AD amplification module 9 converts the voltage signal output by the pre-amplification module 6 into a digital signal.
As shown in fig. 3, in order to detect the biochemical reaction container 4, after the colorimetric disc 2 and the code disc 5 coaxially rotate, the AD amplifying module 9 in the signal acquisition system 3 records a signal passing through the serial number optocoupler when the code disc 5 rotates, and meanwhile, the AD amplifying module 9 in the signal acquisition system 3 records a signal when the biochemical reaction container 4 passes through light. The tooth grooves of the code disc 5 are in one-to-one correspondence with the positions of the biochemical reaction containers 4 in the color comparison disc 2. As can be seen from fig. 3, the slope of the plateau of the signal scan curve is almost 0, which indicates that the parallelism of the light-transmitting surface of the biochemical reaction vessel is good. And the ordinate value corresponding to the signal scanning curve plateau represents the optical energy value after the biochemical reaction vessel is penetrated. When the biochemical reaction container is placed (shielded) or not placed (penetrated), the corresponding optical energy values are V1 and V2, and V1/V2 is the transmittance of the biochemical reaction container.
As shown in fig. 4, the display storage system detects the width t0 of the signal scanning curve in the plateau, the width t1 of the rising edge, and the width t2 of the falling edge, and when the rotation speed of the code wheel 5 is v, the width W0 (mm) =v·t0 of the effective light-transmitting surface of the biochemical reaction container 4, the wall thickness w1=v·t1 on one side of the light-transmitting surface, and the wall thickness w2=v·t2 on the other side.
As shown in fig. 5, when the signal scanning curve is detected as a slope, the absolute value of the slope is proportional to the parallelism of the light-transmitting surface when detecting the biochemical reaction container 4; when the slope is not equal to 0, indicating that the light-transmitting surface of the biochemical reaction container is not parallel or at least one surface is not perpendicular to the incident light of the optical signal provided by the signal acquisition system; when the slope is 0, it means that the two surfaces of the light-transmitting surface are parallel and perpendicular to the incident light.
As shown in fig. 6, when a pit is detected in the middle of the signal scanning curve for detecting the biochemical reaction container 4, it indicates that the light-transmitting surface of the biochemical reaction container is contaminated or scratched, and the width w3=v·t3, wherein: t3 is the width of the pit, which can indirectly reflect the size of the contaminant or scratch.
The foregoing embodiments may be partially modified in numerous ways by those skilled in the art without departing from the principles and spirit of the invention, the scope of which is defined in the claims and not by the foregoing embodiments, and all such implementations are within the scope of the invention.
Claims (5)
1. A detection method based on a detection system for detecting the quality of a biochemical reaction container, which is characterized in that the system comprises: a signal acquisition system that is used for giving square wave cycle rotation signal's when the code wheel rotates serial number opto-coupler, code wheel, with code wheel coaxial coupling's colorimetric disk, the biochemical reaction container that awaits measuring and the positive contrast color disk that sets up on the colorimetric disk, wherein: the biochemical reaction container is selectively arranged on the color palette, so that a signal acquisition system acquires signals from the serial number optocoupler, the signals are selectively shielded by the biochemical reaction container, and the quality detection result of the biochemical reaction container is obtained by calculating the corresponding detected optical signals;
the quality detection result comprises the light transmittance of the biochemical reaction container, the width of the effective light transmission surface, the wall thickness at two sides of the light transmission surface, the parallelism of the light transmission surface, scratches on the light transmission surface and the size of pollutants;
the detection method comprises the following steps: the method comprises the steps of acquiring energy of different optical signals before and after a biochemical reaction container is placed, calculating to obtain the ratio, namely the light transmittance, calculating to obtain the width of an effective light transmission surface of the biochemical reaction container by detecting the width of a flat period of an optical signal scanning curve, calculating to obtain the wall thicknesses of two sides of the light transmission surface of the biochemical reaction container by detecting the width of a rising edge and a falling edge of a signal scanning curve, calculating to obtain the parallelism of the light transmission surface of the biochemical reaction container by detecting the slope of the flat period of the signal scanning curve, calculating to obtain the scratch or pollutant size of the light transmission surface of the biochemical reaction container by detecting the width of a pit of the signal scanning curve, and finally comparing the calculated result with a threshold value to obtain a quality detection result;
the width w0=v·t0 of the effective light-transmitting surface, where: t0 is the width of the signal scanning curve in the stage of the platform, and v is the rotation speed of the code disc;
the wall thickness of the two sides of the light-transmitting surface is W1=v.t1, and W2=v.t2, wherein: t1 and t2 are widths t2 of rising edges and falling edges of a signal scanning curve platform period respectively, and upsilon is the rotation speed of the code disc;
when the detected signal scanning curve is an oblique line, the absolute value of the slope of the oblique line is in direct proportion to the parallelism of the light-transmitting surface; when the slope is not equal to 0, indicating that the light-transmitting surface of the biochemical reaction container is not parallel or at least one surface is not perpendicular to the incident light of the optical signal provided by the signal acquisition system; when the slope is 0, two surfaces of the transparent surface are parallel and perpendicular to the incident light;
when a section of pit is detected in the middle of the signal scanning curve, the transparent surface of the biochemical reaction container is polluted or scratched, and the width W3=v.t3, wherein: t3 is the width of the pit, and v is the rotation speed of the code disc.
2. The method of claim 1, wherein the signal acquisition system comprises: illumination module, pre-amplification module, AD amplification module and calculation module, wherein: the illumination module is arranged in the color comparison disc and is opposite to the photocell module positioned outside the color comparison disc, and light emitted by the illumination module irradiates the photocell module through the biochemical reaction container to be detected and the grating; the photoelectric cell module converts the light signal into a current signal, the pre-amplifying module converts the current signal output by the photoelectric cell into a voltage signal, the AD amplifying module amplifies the voltage signal and converts the voltage signal into a digital signal, and the calculating module calculates and obtains a quality detection result according to the time width, the intensity and the code wheel rotating speed of the digital signal.
3. The method of claim 2, wherein a lens and a mirror are disposed between the illumination module and the photocell module to adjust the length of the optical path.
4. The detection method according to claim 1, wherein the signal acquisition system is further provided with a change curve display storage system for drawing, displaying and deriving the optical signal, the display storage system comprising: the display module is used for displaying the delay curve acquired by the information acquisition system and the storage module is used for storing the delay curve signal acquired by the signal acquisition system.
5. The detecting method according to claim 4, wherein the quality detecting threshold is built in the memory module, and the quality detecting result is compared with the quality detecting threshold and the comparison result is outputted to the display module after the signal collecting system calculates the quality detecting result.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810256321.6A CN108180950B (en) | 2018-03-27 | 2018-03-27 | System and method for detecting quality of biochemical reaction container |
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| CN201810256321.6A CN108180950B (en) | 2018-03-27 | 2018-03-27 | System and method for detecting quality of biochemical reaction container |
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| CN108180950A CN108180950A (en) | 2018-06-19 |
| CN108180950B true CN108180950B (en) | 2024-03-19 |
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| CN110174067B (en) * | 2019-07-01 | 2024-03-12 | 上海科华实验系统有限公司 | Method and system for checking center offset of code disc of biochemical analyzer |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07140089A (en) * | 1993-11-16 | 1995-06-02 | Hitachi Electron Eng Co Ltd | Device for detecting scratch flaw on magnetic disc |
| CN102539346A (en) * | 2011-12-14 | 2012-07-04 | 深圳市尚荣医疗股份有限公司 | Optical system of full-automatic biochemical analyzer |
| CN102713637A (en) * | 2010-01-25 | 2012-10-03 | 株式会社日立高新技术 | Automatic analyzer |
| CN205484075U (en) * | 2016-03-30 | 2016-08-17 | 长春赛诺迈德医学技术有限责任公司 | Cell signal pickup assembly and biochemical analysis appearance |
| CN106872414A (en) * | 2017-01-22 | 2017-06-20 | 江西特康科技有限公司 | The cuvette method of inspection and device |
-
2018
- 2018-03-27 CN CN201810256321.6A patent/CN108180950B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07140089A (en) * | 1993-11-16 | 1995-06-02 | Hitachi Electron Eng Co Ltd | Device for detecting scratch flaw on magnetic disc |
| CN102713637A (en) * | 2010-01-25 | 2012-10-03 | 株式会社日立高新技术 | Automatic analyzer |
| CN102539346A (en) * | 2011-12-14 | 2012-07-04 | 深圳市尚荣医疗股份有限公司 | Optical system of full-automatic biochemical analyzer |
| CN205484075U (en) * | 2016-03-30 | 2016-08-17 | 长春赛诺迈德医学技术有限责任公司 | Cell signal pickup assembly and biochemical analysis appearance |
| CN106872414A (en) * | 2017-01-22 | 2017-06-20 | 江西特康科技有限公司 | The cuvette method of inspection and device |
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| CN108180950A (en) | 2018-06-19 |
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