CN108165487A - A kind of bacterium high-efficiency fermenting tank - Google Patents

A kind of bacterium high-efficiency fermenting tank Download PDF

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Publication number
CN108165487A
CN108165487A CN201810230377.4A CN201810230377A CN108165487A CN 108165487 A CN108165487 A CN 108165487A CN 201810230377 A CN201810230377 A CN 201810230377A CN 108165487 A CN108165487 A CN 108165487A
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China
Prior art keywords
tank
cover
test
bacterium
paddle
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Granted
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CN201810230377.4A
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Chinese (zh)
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CN108165487B (en
Inventor
何晔
周伟
陈彦海
周广坤
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JINING SENLI BIOLOGICAL TECHNOLOGY Co Ltd
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JINING SENLI BIOLOGICAL TECHNOLOGY Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/18Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/38Caps; Covers; Plugs; Pouring means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
    • C12M27/08Stirrer or mobile mixing elements with different stirrer shapes in one shaft or axis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/32Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/38Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of metabolites or enzymes in the cells

Abstract

The invention discloses a kind of bacterium high-efficiency fermenting tanks,It is equipped between outer tank body and inner tank body and thermostatically controlled heat insulation module is carried out to the temperature in inner tank body,Inner tank body inner wall is equipped with heating component,Inner tank body one end is equipped with the mixing component and inflatable component stretched into inside inner tank body,Outer tank body is also fixed with operation cabinet outside one end,Operation cabinet is equipped with parameter set element,Test suite is additionally provided with outside the other end of outer tank body,Test suite is fixed on side cover,The fermentation tank of the present invention is easy to use,Antifoaming agent can very easily be investigated,Iron ion,Condensed water,Influence of the factors such as air mass flow to engineering bacteria cell enzyme activity,Concentration of substrate can also be investigated,Temperature,pH,Ionic strength,Metal ion,Influence of the factors such as production concentration to enzymatic reaction speed,It lays the first stone for large-scale production,Temperature precise control of the present invention,Monitoring is convenient,Side cover, which facilitates, to be dismounted,It can facilitate and its inside is cleaned,Prevent cross influence.

Description

A kind of bacterium high-efficiency fermenting tank
Technical field
The present invention relates to a kind of bacterium high-efficiency fermenting tanks, belong to Zymolysis Equipment technical field.
Background technology
At present, with round pcr from the gene that pichia methanolica GS115 genomic DNAs are template amplification hydrogenlyase, Using fusion expression type carrier T technology, formate dehydrogenase gene is reconstituted in the disulfide bond formation protein D sbA of erasure signal peptide Downstream, make hydrogenlyase amalgamation and expression in the carboxyl terminal of DsbA.It is acted on by means of the molten rush folding of DsbA, makes zymoprotein High-activity expression, the formate dehydrogenase enzyme engineering bacteria that can be obtained has generates NADH and carbon dioxide by substrate of formic acid NAD+ Ability.
When studying formate dehydrogenase enzyme engineering bacteria, need collect with highly-solid selectively carbonyl reductase or The amino acid sequence of alcohol dehydrogenase designs encoding gene with the preference codons of Escherichia coli, after PCR amplification, uses amalgamation and expression Type carrier T constructing technology obtains the genetic engineering bacterium of reductase, and quantity is at 15 or more.Activity expression respectively investigates engineering bacteria The light of 3 hydroxyl ethyl acetate of product 4- chlorine is prepared using model substrates 4- chloroacetyl acetacetic esters and NADH as substrate reduction Learn purity.Hydrogenlyase and carbonyl reductase are reconstituted on same plasmid vector, investigate engineering bacteria with formic acid and 4- chloroethenes Ethyl acetoacetic acid ethyl ester prepares the stereoselectivity of 3 hydroxyl ethyl acetate of 4- chlorine for substrate.
When studying Optimal Expression condition, need to investigate the factors such as different carbon source, nitrogen source, additive to engineering bacteria cellular enzymes Influence living makes zymoprotein that high biological activity be presented under the expression condition of optimization, and target is prepared to improve engineering bacteria cell The ability of product.In pilot scale research is carried out, need the culturing engineering bacterium cell on 300L fermentation tanks, so as to investigate antifoaming agent, Influence of the factors such as iron ion, condensed water, air mass flow to engineering bacteria cell enzyme activity lays the first stone for large-scale production.
But current ferment tank is less efficient, when fermentation, is difficult to investigating antifoaming agent, iron ion, condensed water, sky The factors such as throughput carry out Validity Test and control, and therefore, it is necessary to design a kind of efficient bacterium high-efficiency fermenting tank.
The present invention in view of the above problems, provide a kind of bacterium high-efficiency fermenting tank, be difficult to when improving fermentation to investigate antifoaming agent, The factors such as iron ion, condensed water, air mass flow carry out Validity Test and the convenience of control.
Invention content
To achieve the above object, the present invention provides following technical solution:A kind of bacterium high-efficiency fermenting tank, which is characterized in that It includes outer tank body, inner tank body, heating component, heat insulation module, mixing component, inflatable component, parameter set element and test group Part, wherein, the inner tank body is arranged on inside the outer tank body, and is provided between the outer tank body and the inner tank body internally Temperature in tank body carries out thermostatically controlled heat insulation module, and the heating component is provided on the inner wall of the inner tank body, described One end of inner tank body is provided with the mixing component and the inflatable component stretched into inside the inner tank body, one end of the outer tank body Outside is further fixedly arranged on operation cabinet, and the parameter set element, the other end of the outer tank body are provided in the operation cabinet Outside is additionally provided with the test suite, and the test suite is fixed on side cover, and the side cover is removably arranged on described The other end of outer tank body.
Further, preferably, the heating component use electric heating wire, the heat insulation module include Heat preservation pipe and Thermal insulation medium, the thermal insulation medium are filled between the outer tank body and inner tank body, and disk is equipped with institute on the inner wall of the outer tank body Heat preservation pipe is stated, the Heat preservation pipe is heated and kept the temperature to the thermal insulation medium.
Further, preferably, the inflatable component includes inflator pump, disc for air inflation and air inflation nozzle, the inflator pump is fixed In the operation cabinet, the disc for air inflation is fixedly installed on one end inner wall of the inner tank body, is provided on the disc for air inflation Multiple air inflation nozzles, the disc for air inflation are connected with the inflator pump.
Further, it is described preferably, the parameter set element includes parameter setting display screen and parameter set button Parameter set button includes at least aeration quantity, inflation rate, temperature, mixing speed, mixing time.
Further, preferably, the mixing component includes stirring motor, shaft coupling, paddle one, paddle two and stirs Leaf three is mixed, wherein, the stirring motor is fixed in the operation cabinet, and the stirring motor is connected by the shaft coupling Be provided with agitating shaft, the agitating shaft is stretched into inside the inner tank body, be provided on the agitating shaft from different mixing directions into Paddle one, paddle two and the paddle three of row stirring.
Further, preferably, the paddle one is be arranged on agitating shaft two groups, and one structure of the paddle is set as Substance in inner tank body is stirred into the radial outside to inner tank body, two structure of paddle is set as stirring the substance in inner tank body inwardly The radially inner side of tank body, three structure of paddle are set as stirring the substance in inner tank body into the both ends to inner tank body.
Further, preferably, the test suite includes testing tube, test sensor group, testing tube fixed seat, control Line, test parameter display screen and test controller, wherein, the testing tube is fixed on the side using the testing tube fixed seat It covers, the outer tank body is provided with connecting flange close to one end of the test suite, and the side cover is removably arranged on On the connecting flange, it is sticked on the testing tube and the test that the required parameter of the state of fermented material is monitored is sensed Device group, for the test sensor group by the control line connecting test controller, the test controller is arranged on the side It covers, the test parameter display screen being connect with the controller is additionally provided on the side cover.
Further, preferably, being additionally provided with clump weight on the side cover.
Further, preferably, the bottom of the side cover is provided with idler wheel, the bottom sides of the side cover, which are additionally provided with, determines Position shoulder, the bottom of the outer tank body position the corresponding positioning seat in shoulder position with described, are additionally provided with engaging lug on the side cover, institute It states engaging lug and sealed set is connect with the connecting flange in the outer tank body, the bottom of the outer tank body is provided with multiple supports Seat.
Compared with prior art, the beneficial effects of the invention are as follows:
The fermentation tank of the present invention is easy to use, can very easily investigate antifoaming agent, iron ion, condensed water, air stream Amount etc. influence of the factors to engineering bacteria cell enzyme activity, can also investigate concentration of substrate, temperature, pH, ionic strength, metal ion, Influence of the factors such as production concentration to enzymatic reaction speed lays the first stone for large-scale production, the temperature of fermentation tank of the invention Precise control, monitoring is convenient, meanwhile, side cover, which facilitates, to be dismounted, and can facilitate and its inside is cleaned, and prevents from intersecting shadow It rings.
Description of the drawings
Fig. 1 is a kind of structure diagram of bacterium high-efficiency fermenting tank of the present invention;
Fig. 2 is a kind of structure diagram of the bacterium of the present invention with high-efficiency fermenting tank when dismantling;
Wherein, 1, outer tank body, 2, support base, 3, Heat preservation pipe, 4, thermal insulation medium, 5, inflator pump, 6, operation cabinet, 7, ginseng Number setting display screens, 8, parameter set button, 9, stirring motor, 10, disc for air inflation, 11, air inflation nozzle, 12, shaft coupling, 13, stirring Ye Yi, 14, paddle two, 15, paddle three, 16, testing tube, 17, test sensor, 18, testing tube fixed seat, 19, control Line, 20, test parameter display screen, 21, test controller, 22, clump weight, 23, idler wheel, 24, positioning shoulder, 25, positioning seat, 26, Heating component
Specific embodiment
Below in conjunction with the attached drawing in the embodiment of the present invention, the technical solution in the embodiment of the present invention is carried out clear, complete Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, instead of all the embodiments.It is based on Embodiment in the present invention, those of ordinary skill in the art are obtained every other without making creative work Embodiment shall fall within the protection scope of the present invention.
- 2 are please referred to Fig.1, the present invention provides a kind of technical solution:A kind of bacterium high-efficiency fermenting tank, which is characterized in that it is wrapped Include outer tank body 1, inner tank body, heating component 26, heat insulation module, mixing component, inflatable component, parameter set element and test group Part, wherein, the inner tank body is arranged on inside the outer tank body 1, and is provided between the outer tank body and the inner tank body pair Temperature in inner tank body carries out thermostatically controlled heat insulation module, and the heating component, institute are provided on the inner wall of the inner tank body The one end for stating inner tank body is provided with the mixing component and the inflatable component stretched into inside the inner tank body, and the one of the outer tank body End is external to be further fixedly arranged on operation cabinet 6, is provided with the parameter set element in the operation cabinet 6, the outer tank body it is another The test suite is additionally provided with outside one end, the test suite is fixed on side cover, and the side cover is removably arranged on The other end of the outer tank body.
Wherein, the heating component uses electric heating wire, and the heat insulation module includes Heat preservation pipe 3 and thermal insulation medium 4, The thermal insulation medium 4 is filled between the outer tank body 1 and inner tank body, and disk is equipped with the heat preservation on the inner wall of the outer tank body 1 Heating tube 3, the Heat preservation pipe 3 are heated and are kept the temperature to the thermal insulation medium 4.
As preferred embodiment, the inflatable component includes inflator pump 5, disc for air inflation 10 and air inflation nozzle 11, the inflation Pump 5 is fixed in the operation cabinet 6, and the disc for air inflation 10, the inflation are fixedly installed on one end inner wall of the inner tank body Multiple air inflation nozzles 11 are provided on disk 10, the disc for air inflation 10 is connected with the inflator pump 5.The parameter set element includes ginseng Number setting display screen 7 and parameter set button 8, the parameter set button include at least aeration quantity, inflation rate, temperature, stir Mix speed, mixing time.
The mixing component includes stirring motor 9, shaft coupling 12, paddle 1, paddle 2 14 and paddle 3 15, Wherein, the stirring motor 9 is fixed in the operation cabinet, and the stirring motor 9 is set by the shaft coupling 12 connection Agitating shaft is equipped with, the agitating shaft is stretched into inside the inner tank body, is provided on the agitating shaft from different mixing directions and carried out Paddle 1, paddle 2 14 and the paddle 3 15 of stirring.
As more preferably embodiment, the paddle 1 is be arranged on agitating shaft two groups, and one structure of the paddle is set For the substance in inner tank body to be stirred to the radial outside to inner tank body, 2 14 structure of paddle is set as stirring the substance in inner tank body To the radially inner side of inner tank body, 3 15 structure of paddle is set as stirring the substance in inner tank body into the both ends to inner tank body.
In addition, the test suite include testing tube 16, test sensor group 17, testing tube fixed seat 18, control line 19, Test parameter display screen 20 and test controller 21, wherein, the testing tube 16 is fixed on institute using the testing tube fixed seat It states on side cover, the outer tank body is provided with connecting flange close to one end of the test suite, and the side cover is removably set It puts on the connecting flange, be sticked the test being monitored to the required parameter of the state of fermented material on the testing tube Sensor group 17, by the 19 connecting test controller of control line, the test controller 21 is set the test sensor group It puts on the side cover, the test parameter display screen 20 being connect with the controller, test sensing is additionally provided on the side cover Device group 17 includes at least temperature sensor, PH sensor, the substrate concentration sensor of required measurement, ionic strength sensor, gold Belong to ion transducer and the production concentration sensor tested.
In order to ensure the balance of side cover movement, clump weight 22 is additionally provided on the side cover.The bottom of the side cover is set Idler wheel 23 is equipped with, the bottom sides of the side cover are additionally provided with positioning shoulder 24, the bottom of the outer tank body and the positioning shoulder position Corresponding positioning seat 25 is put, engaging lug, the engaging lug and the connecting flange in the outer tank body are additionally provided on the side cover Sealed set is connected, the bottom of the outer tank body is provided with multiple support bases 2.
The fermentation tank of the present invention is easy to use, can very easily investigate antifoaming agent, iron ion, condensed water, air stream Amount etc. influence of the factors to engineering bacteria cell enzyme activity, can also investigate concentration of substrate, temperature, pH, ionic strength, metal ion, Influence of the factors such as production concentration to enzymatic reaction speed lays the first stone for large-scale production, the temperature of fermentation tank of the invention Precise control, monitoring is convenient, meanwhile, side cover, which facilitates, to be dismounted, and can facilitate and its inside is cleaned, and prevents from intersecting shadow It rings.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of variations, modification, replace And modification, the scope of the present invention is defined by the appended.

Claims (9)

1. a kind of bacterium high-efficiency fermenting tank, which is characterized in that it includes outer tank body, inner tank body, heating component, heat insulation module, stirs Component, inflatable component, parameter set element and test suite are mixed, wherein, the inner tank body is arranged on inside the outer tank body, and It is provided between the outer tank body and the inner tank body and thermostatically controlled heat insulation module is carried out to the temperature in inner tank body, it is described interior Be provided with the heating component on the inner wall of tank body, one end of the inner tank body be provided with stretch into it is described inside the inner tank body Mixing component and inflatable component are further fixedly arranged on operation cabinet outside one end of the outer tank body, are provided in the operation cabinet The parameter set element is additionally provided with the test suite outside the other end of the outer tank body, and the test suite is fixed On side cover, the side cover is removably arranged on the other end of the outer tank body.
2. a kind of bacterium high-efficiency fermenting tank according to claim 1, it is characterised in that:The heating component uses electrical heating Silk, the heat insulation module include Heat preservation pipe and thermal insulation medium, and the thermal insulation medium is filled in the outer tank body and inner tank body Between, disk is equipped with the Heat preservation pipe on the inner wall of the outer tank body, and the Heat preservation pipe carries out the thermal insulation medium Heating and heat preservation.
3. a kind of bacterium high-efficiency fermenting tank according to claim 1, it is characterised in that:The inflatable component includes inflation Pump, disc for air inflation and air inflation nozzle, the inflator pump are fixed in the operation cabinet, are fixedly installed on one end inner wall of the inner tank body There is the disc for air inflation, multiple air inflation nozzles are provided on the disc for air inflation, the disc for air inflation is connected with the inflator pump.
4. a kind of bacterium high-efficiency fermenting tank according to claim 1, it is characterised in that:The parameter set element includes ginseng Number setting display screen and parameter set button, the parameter set button include at least aeration quantity, inflation rate, temperature, stirring Speed, mixing time.
5. a kind of bacterium high-efficiency fermenting tank according to claim 1, it is characterised in that:The mixing component includes stirring electricity Machine, shaft coupling, paddle one, paddle two and paddle three, wherein, the stirring motor is fixed at the operation cabinet On, the stirring motor is connected with agitating shaft by the shaft coupling, and the agitating shaft is stretched into inside the inner tank body, institute State paddle one, paddle two and the paddle three for being provided on agitating shaft and being stirred from different mixing directions.
6. a kind of bacterium high-efficiency fermenting tank according to claim 5, it is characterised in that:The paddle one is stirred to be arranged on Mix axis two groups, and one structure of the paddle is set as the substance in inner tank body stirring radial outside to inner tank body, the stirring Two structure of leaf is set as the substance in inner tank body stirring radially inner side to inner tank body, and three structure of paddle is set as will be in inner tank body Substance stirs the both ends to inner tank body.
7. a kind of bacterium high-efficiency fermenting tank according to claim 1, it is characterised in that:The test suite includes test Pipe, test sensor group, testing tube fixed seat, control line, test parameter display screen and test controller, wherein, the test Pipe is fixed on using the testing tube fixed seat on the side cover, and the outer tank body is set close to one end of the test suite There is connecting flange, the side cover is removably arranged on the connecting flange, and being sticked on the testing tube has to fermented material State the test sensor group that is monitored of required parameter, the test sensor group passes through the control line connecting test Controller, the test controller are arranged on the side cover, and the survey being connect with the controller is additionally provided on the side cover Try parameter display screen.
8. a kind of bacterium high-efficiency fermenting tank according to claim 7, it is characterised in that:Counterweight is additionally provided on the side cover Block.
9. a kind of bacterium high-efficiency fermenting tank according to any one of claims 1 to 8, it is characterised in that:The bottom of the side cover Portion is provided with idler wheel, and the bottom sides of the side cover are additionally provided with positioning shoulder, the bottom of the outer tank body and the positioning shoulder position Corresponding positioning seat is put, engaging lug is additionally provided on the side cover, the engaging lug connects with the connecting flange in the outer tank body Sealed set is connect, the bottom of the outer tank body is provided with multiple support bases.
CN201810230377.4A 2018-03-20 2018-03-20 High-efficient fermentation cylinder is used to fungus Active CN108165487B (en)

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CN108165487B CN108165487B (en) 2022-02-01

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