CN108114086A - A kind of throat lozenge and preparation method thereof - Google Patents
A kind of throat lozenge and preparation method thereof Download PDFInfo
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- CN108114086A CN108114086A CN201711256464.9A CN201711256464A CN108114086A CN 108114086 A CN108114086 A CN 108114086A CN 201711256464 A CN201711256464 A CN 201711256464A CN 108114086 A CN108114086 A CN 108114086A
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- 239000007937 lozenge Substances 0.000 title claims abstract description 50
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 230000000694 effects Effects 0.000 claims abstract description 83
- 244000029687 Wikstroemia indica Species 0.000 claims abstract description 18
- 241000143476 Bidens Species 0.000 claims abstract description 16
- 235000010662 Bidens pilosa Nutrition 0.000 claims abstract description 16
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 124
- 239000003795 chemical substances by application Substances 0.000 claims description 112
- 239000000284 extract Substances 0.000 claims description 96
- 235000019441 ethanol Nutrition 0.000 claims description 50
- 239000000706 filtrate Substances 0.000 claims description 48
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 48
- 239000012141 concentrate Substances 0.000 claims description 47
- 238000002156 mixing Methods 0.000 claims description 42
- 239000000243 solution Substances 0.000 claims description 38
- 230000008338 local blood flow Effects 0.000 claims description 37
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 36
- 239000012153 distilled water Substances 0.000 claims description 36
- 230000000149 penetrating effect Effects 0.000 claims description 36
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 27
- 238000001914 filtration Methods 0.000 claims description 26
- 239000000047 product Substances 0.000 claims description 25
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 24
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 24
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 24
- 238000002390 rotary evaporation Methods 0.000 claims description 24
- 238000007873 sieving Methods 0.000 claims description 24
- 238000000605 extraction Methods 0.000 claims description 22
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical class [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 claims description 22
- 239000002245 particle Substances 0.000 claims description 22
- 229920001661 Chitosan Polymers 0.000 claims description 13
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 13
- 239000008101 lactose Substances 0.000 claims description 13
- ODKSFYDXXFIFQN-BYPYZUCNSA-N L-arginine Chemical compound OC(=O)[C@@H](N)CCCN=C(N)N ODKSFYDXXFIFQN-BYPYZUCNSA-N 0.000 claims description 12
- 229930064664 L-arginine Natural products 0.000 claims description 12
- 235000014852 L-arginine Nutrition 0.000 claims description 12
- NQTADLQHYWFPDB-UHFFFAOYSA-N N-Hydroxysuccinimide Chemical compound ON1C(=O)CCC1=O NQTADLQHYWFPDB-UHFFFAOYSA-N 0.000 claims description 12
- 244000288157 Passiflora edulis Species 0.000 claims description 12
- 235000000370 Passiflora edulis Nutrition 0.000 claims description 12
- 238000005119 centrifugation Methods 0.000 claims description 12
- 230000006837 decompression Effects 0.000 claims description 12
- 238000007599 discharging Methods 0.000 claims description 12
- 239000006166 lysate Substances 0.000 claims description 12
- 235000019359 magnesium stearate Nutrition 0.000 claims description 12
- 238000003825 pressing Methods 0.000 claims description 12
- 238000005507 spraying Methods 0.000 claims description 12
- 239000006228 supernatant Substances 0.000 claims description 12
- 239000007864 aqueous solution Substances 0.000 claims description 11
- 238000005469 granulation Methods 0.000 claims description 11
- 230000003179 granulation Effects 0.000 claims description 11
- 239000008188 pellet Substances 0.000 claims description 11
- 239000003208 petroleum Substances 0.000 claims description 11
- 239000001866 hydroxypropyl methyl cellulose Substances 0.000 claims description 7
- 229920003088 hydroxypropyl methyl cellulose Polymers 0.000 claims description 7
- 235000010979 hydroxypropyl methyl cellulose Nutrition 0.000 claims description 7
- UFVKGYZPFZQRLF-UHFFFAOYSA-N hydroxypropyl methyl cellulose Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(O)C(CO)O3)O)C(CO)O2)O)C(CO)O1 UFVKGYZPFZQRLF-UHFFFAOYSA-N 0.000 claims description 7
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 claims description 2
- LKDRXBCSQODPBY-AMVSKUEXSA-N L-(-)-Sorbose Chemical compound OCC1(O)OC[C@H](O)[C@@H](O)[C@@H]1O LKDRXBCSQODPBY-AMVSKUEXSA-N 0.000 claims 1
- 239000000843 powder Substances 0.000 claims 1
- 238000002604 ultrasonography Methods 0.000 claims 1
- 201000007100 Pharyngitis Diseases 0.000 abstract description 18
- 206010011224 Cough Diseases 0.000 abstract description 10
- 206010062717 Increased upper airway secretion Diseases 0.000 abstract description 7
- 208000026435 phlegm Diseases 0.000 abstract description 7
- 208000024891 symptom Diseases 0.000 abstract description 6
- 239000004480 active ingredient Substances 0.000 abstract description 5
- 206010068319 Oropharyngeal pain Diseases 0.000 abstract description 4
- ZYGHJZDHTFUPRJ-UHFFFAOYSA-N benzo-alpha-pyrone Natural products C1=CC=C2OC(=O)C=CC2=C1 ZYGHJZDHTFUPRJ-UHFFFAOYSA-N 0.000 abstract description 4
- 235000001671 coumarin Nutrition 0.000 abstract description 4
- 150000004775 coumarins Chemical class 0.000 abstract description 4
- 150000004676 glycans Chemical class 0.000 abstract description 4
- 239000004615 ingredient Substances 0.000 abstract description 4
- 229920001282 polysaccharide Polymers 0.000 abstract description 4
- 239000005017 polysaccharide Substances 0.000 abstract description 4
- 230000008961 swelling Effects 0.000 abstract description 4
- 229930003935 flavonoid Natural products 0.000 abstract description 3
- 150000002215 flavonoids Chemical class 0.000 abstract description 3
- 235000017173 flavonoids Nutrition 0.000 abstract description 3
- 230000007774 longterm Effects 0.000 abstract description 3
- 241000196324 Embryophyta Species 0.000 abstract description 2
- 208000008454 Hyperhidrosis Diseases 0.000 abstract description 2
- 230000003110 anti-inflammatory effect Effects 0.000 abstract description 2
- 208000037976 chronic inflammation Diseases 0.000 abstract description 2
- 230000006020 chronic inflammation Effects 0.000 abstract description 2
- JRHMMVBOTXEHGJ-UHFFFAOYSA-N daphnoretin Chemical compound C1=CC(=O)OC2=CC(OC3=CC=4C=C(C(=CC=4OC3=O)O)OC)=CC=C21 JRHMMVBOTXEHGJ-UHFFFAOYSA-N 0.000 abstract description 2
- UJRSXAFBORHKBS-UHFFFAOYSA-N daphnoretin Natural products COC1=CC2C=C(Oc3ccc4C=CC(=O)Oc4c3)C(=O)OC2C=C1O UJRSXAFBORHKBS-UHFFFAOYSA-N 0.000 abstract description 2
- 208000013219 diaphoresis Diseases 0.000 abstract description 2
- 230000001939 inductive effect Effects 0.000 abstract description 2
- 230000001151 other effect Effects 0.000 abstract description 2
- LFBIHCZSRPAPHS-UHFFFAOYSA-N rutamontine Natural products COc1cc2OC(=O)C(=Cc2cc1O)Oc3ccc4C=CC(=O)Oc4c3 LFBIHCZSRPAPHS-UHFFFAOYSA-N 0.000 abstract description 2
- 229940126680 traditional chinese medicines Drugs 0.000 abstract description 2
- 235000008504 concentrate Nutrition 0.000 description 33
- 230000000052 comparative effect Effects 0.000 description 14
- 239000007788 liquid Substances 0.000 description 12
- 239000003814 drug Substances 0.000 description 11
- 238000004043 dyeing Methods 0.000 description 11
- 238000002137 ultrasound extraction Methods 0.000 description 11
- 235000020985 whole grains Nutrition 0.000 description 10
- 238000000502 dialysis Methods 0.000 description 9
- 238000003756 stirring Methods 0.000 description 9
- -1 stirring 18h Substances 0.000 description 5
- 235000013339 cereals Nutrition 0.000 description 4
- 210000000214 mouth Anatomy 0.000 description 4
- 229920003091 Methocel™ Polymers 0.000 description 3
- 210000000170 cell membrane Anatomy 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 3
- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 3
- 238000009736 wetting Methods 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 206010000269 abscess Diseases 0.000 description 2
- 239000011149 active material Substances 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
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- 235000009508 confectionery Nutrition 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 230000001965 increasing effect Effects 0.000 description 2
- 230000004054 inflammatory process Effects 0.000 description 2
- 210000000867 larynx Anatomy 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 210000004379 membrane Anatomy 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 210000004400 mucous membrane Anatomy 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 230000004936 stimulating effect Effects 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- 206010067484 Adverse reaction Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 244000132059 Carica parviflora Species 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- CHJJGSNFBQVOTG-UHFFFAOYSA-N N-methyl-guanidine Natural products CNC(N)=N CHJJGSNFBQVOTG-UHFFFAOYSA-N 0.000 description 1
- 208000028571 Occupational disease Diseases 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000006838 adverse reaction Effects 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- ZRALSGWEFCBTJO-UHFFFAOYSA-N anhydrous guanidine Natural products NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 208000020670 canker sore Diseases 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- SWSQBOPZIKWTGO-UHFFFAOYSA-N dimethylaminoamidine Natural products CN(C)C(N)=N SWSQBOPZIKWTGO-UHFFFAOYSA-N 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 201000001421 hyperglycemia Diseases 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- DKXULEFCEORBJK-UHFFFAOYSA-N magnesium;octadecanoic acid Chemical compound [Mg].CCCCCCCCCCCCCCCCCC(O)=O DKXULEFCEORBJK-UHFFFAOYSA-N 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 150000003242 quaternary ammonium salts Chemical class 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 230000035922 thirst Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/36—Caryophyllaceae (Pink family), e.g. babysbreath or soapwort
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/83—Thymelaeaceae (Mezereum family), e.g. leatherwood or false ohelo
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/20—Pills, tablets, discs, rods
- A61K9/2004—Excipients; Inactive ingredients
- A61K9/2022—Organic macromolecular compounds
- A61K9/205—Polysaccharides, e.g. alginate, gums; Cyclodextrin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/20—Pills, tablets, discs, rods
- A61K9/2004—Excipients; Inactive ingredients
- A61K9/2022—Organic macromolecular compounds
- A61K9/205—Polysaccharides, e.g. alginate, gums; Cyclodextrin
- A61K9/2054—Cellulose; Cellulose derivatives, e.g. hydroxypropyl methylcellulose
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Epidemiology (AREA)
- Alternative & Traditional Medicine (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
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- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicinal Preparation (AREA)
Abstract
The present invention relates to technical field of traditional Chinese medicines, and in particular to a kind of throat lozenge and preparation method thereof.The present invention has the effect of dispelling wind, heat-clearing, inducing diaphoresis by extracting the active ingredient in beggar-ticks and Wikstroemia indica stem skin, beggar-ticks, can solve the symptoms such as sore-throat, cough, more phlegm, clearing heat, relieving sore-throat；Wikstroemia indica active ingredient is mainly containing flavonoids, Coumarins, polysaccharide etc., Coumarins ingredient daphnoretin is the plant principle active component, Wikstroemia indica stem skin is primarily present in, there is anti-inflammatory, reducing phlegm and resolving masses, swelling and pain relieving and other effects, be conducive to the alleviation of pharyngitis symptom；The present invention constitutes the stressor of throat for throat inflammation, cough, body long-term stress state, bottleneck throat meeting swelling, tear is damaged, the polysaccharide component of radix pseudostellariae has the effects that enhance human body, resisting stress, Immune enhancement, on the one hand throat can be reduced because damage caused by Chronic inflammation is stimulated and coughed, on the other hand enhances the raising of bottleneck throat immune resistance.
Description
Technical field
The present invention relates to technical field of traditional Chinese medicines, and in particular to a kind of throat lozenge and preparation method thereof.
Background technology
Showing that the patient of acute, chronic pharyngitis is increasing through investigation, disease has seriously affected their work and life,
Particularly this industry of teacher, due to the particularity of work, chronic pharyngitis has become their exclusive " occupational disease ", for this purpose, city
Also occurring some on field again and again for treating the drug and health products of pharyngitis, throat lozenge is one kind of wherein usage amount maximum, but
Either Western medicine or Chinese medicine, are not taken by symptom or excessive use, and certain damage can be all caused to body, is especially contained
The throat lozenge of Chinese medicine constituents, since consumer is unfamiliar with traditional Chinese medicine ingredients therein, usually because taking excessive or related taboo
Factor and cause diarrhea, the adverse reactions such as canker sore, not only without alleviate pharyngitis symptom, but aggravated the state of an illness in addition generate
A series of complication.
The throat lozenge of western medicine composition, main component are iodine or quaternary ammonium salt surface active agent, such as Dobell etc., this kind of
Drug contains iodine molecule, and also very big to the irritation of mucous membrane of mouth although activity is big, sterilizing power is strong, long-time service can press down
Normal flora growth inside donsole chamber and throat, upsets internal environment of oral cavity, local flora is caused to lack of proper care, is burst so as to induce oral cavity
The diseases such as ulcer occur.Chinese medicine class throat lozenge is based on traditional Chinese medicine ingredients, such as Waterelon Frost Lozenges, sweets golden throat, compound grass coral
Coral lozenge, Liushen Pills etc. mainly reach " promoting the production of body fluid to quench thirst " effect by stimulating mucous membrane, however, present thoat-soothing drops on the market
The high raw material of the sugar contents such as sucrose, syrup is with the addition of mostly, hyperglycemia population is not appropriate for and eats, and in fact, those mouths
Feel thoat-soothing drops that are sweet, stimulating and be highly detrimental to aditus laryngis health, because sugar part therein is often the training of " bacterium, virus "
Base is supported, substantial amounts of sugar can undoubtedly give the chance that the pathogenic bacteria inside aditus laryngis provide growth and breeding, not simply fail to treatment disease, instead
And diseased possibility can be increased.In addition, some thoat-soothing drops brands for being known as no added sucrose are but with the addition of substantial amounts of synthesis sweet taste
Agent, long-term use can influence people's health, it is impossible to meet modern people to food have it is green and healthy it is no added,
The high requirement of healthy nutritive value.In addition, present pharyngitis patient carries the problem of slight cough and abscess of throat mostly,
But the throat lozenge nowadays produced in the market, for majority to wet one's whistle, based on cough-relieving, eliminating inflammation and expelling toxin function is not strong enough.
Therefore, we more it is necessary to develop a kind of new throat lozenge again, solved nowadays in the market with this
The problems of throat lozenge, the demand with society and relevant department is preferably adapted to this.
The content of the invention
The technical problems to be solved by the invention：For existing market throat lozenge, majority is to wet one's whistle, based on cough-relieving, anti-inflammatory
The problem of function of detoxification is not strong enough, and effect is poor provides a kind of throat lozenge and preparation method thereof.
In order to solve the above technical problems, the present invention is using technical solution as described below：
A kind of throat lozenge, according to the mass fraction, including 7 ~ 8 parts of lactose, 4 ~ 7 parts of D-sorbites, the hydroxyl of 15 ~ 20 parts of mass fractions 6%
Propyl methocel ethanol solution, 2 ~ 4 parts of magnesium stearates, which is characterized in that 20 ~ 30 parts of activity extract A that wet one's whistle are further included,
25 ~ 35 parts of activity extract B that wet one's whistle, 15 ~ 20 parts of shield throat agent, 6 ~ 8 parts of penetrating agents, 3 ~ 5 parts of rush local blood circulation agent.
The activity extract A that wets one's whistle takes beggar-ticks in mass ratio 1：8 add in mass fraction as 70% methanol solution, 25 ~
32 DEG C of 5 ~ 6d of extraction, filter, filtrate decompression are taken to concentrate, obtains concentrate, takes paste in mass ratio 1：10 add in water mixing, successively
It is respectively extracted 3 times with petroleum ether, ethyl acetate, n-butanol, combining extraction liquid, rotary evaporation, must wet one's whistle activity extract A.
The activity extract B that wets one's whistle takes Wikstroemia indica stem skin, crushed 80 mesh sieves, collects sieving particle, and in mass ratio 1：
10 add in the ethyl alcohol that mass fraction is 95%, and in 140 ~ 160W, 1 ~ 1.5h of ultrasonic extraction, filtering takes filtrate, rotary evaporation must moisten
Larynx activity extract B.
The shield throat agent is to take Heterophylly Falsesatarwort Root in mass ratio 1：9 add in distilled water mixing, in 90 ~ 95 DEG C extract 110 ~
120 min, filtering, take filtrate, concentrate, obtain concentrate, in mass ratio 1：8 add in the ethanol solution that mass fraction is 80%, stand
2 ~ 3h takes pellet frozen to dry, and must protect throat agent.
The penetrating agent is to take L-arginine in mass ratio 1：9 are dissolved in distilled water, take lysate in mass ratio 5：1：1
Add in n-hydroxysuccinimide and 1-（3- dimethylamino-propyls）- 3- ethyl-carbodiimide hydrochlorides adjust pH to 6, in 25 ~
28 DEG C of 2 ~ 3h of reaction, obtain product, take chitosan in mass ratio 2：3：10 addition products and mass fraction are water-soluble for 1% AcOH
Liquid, is stirred 18 ~ for 24 hours, and distilled water is dialysed 1.5 ~ 2d, and filtering takes filtrate, is freeze-dried, obtains penetrating agent.
The rush local blood circulation agent is to take passion fruit in mass ratio 1：8 add in the ethyl alcohol that mass fraction is 95%, in
25 ~ 30 DEG C, 30W microwaves dip dyeing 1 ~ 1.5h, 8000r/min centrifugation 10min, take supernatant, are evaporated under reduced pressure, concentration must promote local
Blood circulation agent.
The preparation method includes the following steps：The activity extract A that wets one's whistle is taken, wet one's whistle activity extract B, protects throat agent, promotees to ooze
Agent promotees local blood circulation agent, lactose, D-sorbite, the hydroxypropyl methyl cellulose ethanol solution of mass fraction 6%, stearic acid
Magnesium mixes, spraying granulation, and 16 mesh sieve whole grains are crossed in discharging, collects sieving particle, tabletting, and piece weighs 0.5 ~ 0.55g, and pressing plate is to get profit
Lozenge.
Compared with other methods, advantageous effects are the present invention：
（1）The present invention by extracting the active ingredient in beggar-ticks and Wikstroemia indica stem skin, beggar-ticks have dispelling wind, heat-clearing, inducing diaphoresis it
Effect can solve the symptoms such as sore-throat, cough, more phlegm, clearing heat, relieving sore-throat；Wikstroemia indica active ingredient mainly containing flavonoids, Coumarins,
Polysaccharide etc., Coumarins ingredient daphnoretin are the plant principle active components, are primarily present in Wikstroemia indica stem skin, are had anti-
Inflammation, reducing phlegm and resolving masses, swelling and pain relieving and other effects are conducive to the alleviation of pharyngitis symptom；
（2）The present invention constitutes the stressor of throat, body long-term stress state for throat inflammation, cough, and bottleneck throat can swell
Swollen, tear is damaged, and the polysaccharide component of radix pseudostellariae has the effects that enhance human body, resisting stress, Immune enhancement, on the one hand can be with
Throat is reduced because damage caused by Chronic inflammation is stimulated and coughed, on the other hand enhances the raising of bottleneck throat immune resistance；
（3）The present invention is by preparing a kind of polypeptide with stronger cell membrane penetration capacity, smart ammonia in the active peptides of positively charged
Remaining amino is combined with electronegative cell membrane on sour guanidine radicals and chitosan parent, can be opened close membrane structure, be passed through
It is intracellular and keep its bioactivity that the active material of the alleviation pharyngitis of extraction is imported into throat, and not damaging cells membrane structure and
Function, transfer efficiency is high, without consuming cellular energy；
（4）The present invention promotees the active material of bottleneck throat local blood circulation, predominantly Flavonoid substances by extracting, by wetting one's whistle
The active constituents of medicine that piece mainly wets one's whistle imports cell membrane, accelerates to cycle by local blood, active ingredient is enable to reach rapidly
Laryngopharyngitis position accelerates the volatilization and operating of drug, promotes the performance of drug effect.
Specific embodiment
The activity extract A that wets one's whistle takes beggar-ticks in mass ratio 1：8 add in mass fraction as 70% methanol solution, 25 ~ 32 DEG C
5 ~ 6d is extracted, filters, filtrate decompression is taken to concentrate, obtains concentrate, takes paste in mass ratio 1：10 add in water mixing, use stone successively
Oily ether, ethyl acetate, n-butanol respectively extract 3 times, combining extraction liquid, rotary evaporation, and must wet one's whistle activity extract A.
The activity extract B that wets one's whistle takes Wikstroemia indica stem skin, crushed 80 mesh sieves, collects sieving particle, and in mass ratio 1：10 add
Enter the ethyl alcohol that mass fraction is 95%, in 140 ~ 160W, 1 ~ 1.5h of ultrasonic extraction, filtering takes filtrate, rotary evaporation, must wet one's whistle work
Property extract B.
It is to take Heterophylly Falsesatarwort Root in mass ratio 1 to protect throat agent：9 add in distilled water mixing, and 110 ~ 120 are extracted in 90 ~ 95 DEG C
Min, filtering, takes filtrate, concentrates, obtains concentrate, in mass ratio 1：8 add in mass fraction as 80% ethanol solution, and standing 2 ~
3h takes pellet frozen to dry, and must protect throat agent.
Penetrating agent is to take L-arginine in mass ratio 1：9 are dissolved in distilled water, take lysate in mass ratio 5：1：1 adds in
N-hydroxysuccinimide and 1-（3- dimethylamino-propyls）- 3- ethyl-carbodiimide hydrochlorides adjust pH to 6, in 25 ~ 28 DEG C
2 ~ 3h is reacted, product is obtained, takes chitosan in mass ratio 2：3：10 add in product and mass fraction as 1% AcOH aqueous solutions,
It is stirred 18 ~ for 24 hours, distilled water is dialysed 1.5 ~ 2d, and filtering takes filtrate, is freeze-dried, obtains penetrating agent.
It is to take passion fruit in mass ratio 1 to promote local blood circulation agent：8 add in the ethyl alcohol that mass fractions are 95%, in 25 ~
30 DEG C, 30W microwaves dip dyeing 1 ~ 1.5h, 8000r/min centrifugation 10min, take supernatant, are evaporated under reduced pressure, concentration obtains rush local blood
Cycle agent.
A kind of preparation method of throat lozenge according to the mass fraction, takes 20 ~ 30 parts of activity extract A that wet one's whistle, 25 ~ 35 parts of profits
Larynx activity extract B, 15 ~ 20 parts of shield throat agent, 6 ~ 8 parts of penetrating agents, 3 ~ 5 parts of rush local blood circulation agent, 7 ~ 8 parts of lactose, 4 ~ 7 parts
D-sorbite, the hydroxypropyl methyl cellulose ethanol solution of 15 ~ 20 parts of mass fractions 6%, 2 ~ 4 parts of magnesium stearate mixing, spraying system
16 mesh sieve whole grains are crossed in grain, discharging, collect sieving particle, tabletting, and piece weighs 0.5 ~ 0.55g, and pressing plate is up to throat lozenge.
Embodiment 1
The activity extract A that wets one's whistle takes beggar-ticks in mass ratio 1：8 add in the methanol solution that mass fraction is 70%, and 25 DEG C extract 5d,
It filters, filtrate decompression is taken to concentrate, obtains concentrate, takes paste in mass ratio 1：10 add in water mixing, successively with petroleum ether, acetic acid
Ethyl ester, n-butanol respectively extract 3 times, combining extraction liquid, rotary evaporation, and must wet one's whistle activity extract A.
The activity extract B that wets one's whistle takes Wikstroemia indica stem skin, crushed 80 mesh sieves, collects sieving particle, and in mass ratio 1：10 add
Enter the ethyl alcohol that mass fraction is 95%, in 140W ultrasonic extraction 1h, filtering takes filtrate, rotary evaporation, must wet one's whistle activity extract
B。
It is to take Heterophylly Falsesatarwort Root in mass ratio 1 to protect throat agent：9 add in distilled water mixing, extract 110 min in 90 DEG C, filter,
Filtrate is taken, concentrates, obtains concentrate, in mass ratio 1：8 add in the ethanol solution that mass fraction is 80%, stand 2 ~ 3h, take precipitation cold
It is lyophilized dry, throat agent must be protected.
Penetrating agent is to take L-arginine in mass ratio 1：9 are dissolved in distilled water, take lysate in mass ratio 5：1：1 adds in
N-hydroxysuccinimide and 1-（3- dimethylamino-propyls）- 3- ethyl-carbodiimide hydrochlorides adjust pH to 6, anti-in 25 DEG C
2h is answered, obtains product, takes chitosan in mass ratio 2：3：10 add in product and mass fraction as 1% AcOH aqueous solutions, stirring
18h, distilled water dialysis 1.5d are mixed, filtering takes filtrate, is freeze-dried, obtains penetrating agent.
It is to take passion fruit in mass ratio 1 to promote local blood circulation agent：8 add in the ethyl alcohol that mass fraction is 95%, in 25
DEG C, 30W microwaves dip dyeing 15h, 8000r/min centrifugation 10min, take supernatant, be evaporated under reduced pressure, concentration, obtain rush local blood circulation
Agent.
A kind of preparation method of throat lozenge according to the mass fraction, takes 20 parts of activity extract A that wet one's whistle, 25 parts of activity that wet one's whistle
Extract B, 15 parts of shield throat agent, 6 parts of penetrating agents, 3 parts of rush local blood circulation agent, 7 parts of lactose, 4 parts of D-sorbites, 15 parts of quality
16 mesh sieve whole grains are crossed in the hydroxypropyl methyl cellulose ethanol solution of fraction 6%, 2 parts of magnesium stearate mixing, spraying granulation, discharging,
Sieving particle is collected, tabletting, piece weight 0.5g, pressing plate is up to throat lozenge.
Embodiment 2
The activity extract A that wets one's whistle takes beggar-ticks in mass ratio 1：8 add in the methanol solution that mass fraction is 70%, and 30 DEG C extract 5d,
It filters, filtrate decompression is taken to concentrate, obtains concentrate, takes paste in mass ratio 1：10 add in water mixing, successively with petroleum ether, acetic acid
Ethyl ester, n-butanol respectively extract 3 times, combining extraction liquid, rotary evaporation, and must wet one's whistle activity extract A.
The activity extract B that wets one's whistle takes Wikstroemia indica stem skin, crushed 80 mesh sieves, collects sieving particle, and in mass ratio 1：10 add
Enter the ethyl alcohol that mass fraction is 95%, in 150W ultrasonic extraction 1.2h, filtering takes filtrate, rotary evaporation, the activity that must wet one's whistle extraction
Object B.
It is to take Heterophylly Falsesatarwort Root in mass ratio 1 to protect throat agent：9 add in distilled water mixing, extract 115 min in 93 DEG C, filter,
Filtrate is taken, concentrates, obtains concentrate, in mass ratio 1：8 add in the ethanol solution that mass fraction is 80%, stand 2h, take pellet frozen
It is dry, throat agent must be protected.
Penetrating agent is to take L-arginine in mass ratio 1：9 are dissolved in distilled water, take lysate in mass ratio 5：1：1 adds in
N-hydroxysuccinimide and 1-（3- dimethylamino-propyls）- 3- ethyl-carbodiimide hydrochlorides adjust pH to 6, anti-in 27 DEG C
2h is answered, obtains product, takes chitosan in mass ratio 2：3：10 add in product and mass fraction as 1% AcOH aqueous solutions, stirring
20h, distilled water dialysis 1.7d are mixed, filtering takes filtrate, is freeze-dried, obtains penetrating agent.
It is to take passion fruit in mass ratio 1 to promote local blood circulation agent：8 add in the ethyl alcohol that mass fraction is 95%, in 27
DEG C, 30W microwaves dip dyeing 1.2h, 8000r/min centrifugation 10min, take supernatant, be evaporated under reduced pressure, concentration, obtain rush local blood circulation
Agent.
A kind of preparation method of throat lozenge according to the mass fraction, takes 25 parts of activity extract A that wet one's whistle, 30 parts of activity that wet one's whistle
Extract B, 17 parts of shield throat agent, 7 parts of penetrating agents, 4 parts of rush local blood circulation agent, 7 parts of lactose, 5 parts of D-sorbites, 17 parts of quality
16 mesh sieve whole grains are crossed in the hydroxypropyl methyl cellulose ethanol solution of fraction 6%, 3 parts of magnesium stearate mixing, spraying granulation, discharging,
Sieving particle is collected, tabletting, piece weight 0.52g, pressing plate is up to throat lozenge.
Embodiment 3
The activity extract A that wets one's whistle takes beggar-ticks in mass ratio 1：8 add in the methanol solution that mass fraction is 70%, and 32 DEG C extract 6d,
It filters, filtrate decompression is taken to concentrate, obtains concentrate, takes paste in mass ratio 1：10 add in water mixing, successively with petroleum ether, acetic acid
Ethyl ester, n-butanol respectively extract 3 times, combining extraction liquid, rotary evaporation, and must wet one's whistle activity extract A.
The activity extract B that wets one's whistle takes Wikstroemia indica stem skin, crushed 80 mesh sieves, collects sieving particle, and in mass ratio 1：10 add
Enter the ethyl alcohol that mass fraction is 95%, in 160W ultrasonic extraction 1.5h, filtering takes filtrate, rotary evaporation, the activity that must wet one's whistle extraction
Object B.
It is to take Heterophylly Falsesatarwort Root in mass ratio 1 to protect throat agent：9 add in distilled water mixing, extract 120 min in 95 DEG C, filter,
Filtrate is taken, concentrates, obtains concentrate, in mass ratio 1：8 add in the ethanol solution that mass fraction is 80%, stand 3h, take pellet frozen
It is dry, throat agent must be protected.
Penetrating agent is to take L-arginine in mass ratio 1：9 are dissolved in distilled water, take lysate in mass ratio 5：1：1 adds in
N-hydroxysuccinimide and 1-（3- dimethylamino-propyls）- 3- ethyl-carbodiimide hydrochlorides adjust pH to 6, anti-in 28 DEG C
3h is answered, obtains product, takes chitosan in mass ratio 2：3：10 add in product and mass fraction as 1% AcOH aqueous solutions, stirring
For 24 hours, distilled water dialysis 2d, filtering takes filtrate, is freeze-dried, obtains penetrating agent for mixing.
It is to take passion fruit in mass ratio 1 to promote local blood circulation agent：8 add in the ethyl alcohol that mass fraction is 95%, in 30
DEG C, 30W microwaves dip dyeing 1.5h, 8000r/min centrifugation 10min, take supernatant, be evaporated under reduced pressure, concentration, obtain rush local blood circulation
Agent.
A kind of preparation method of throat lozenge according to the mass fraction, takes 30 parts of activity extract A that wet one's whistle, 35 parts of activity that wet one's whistle
Extract B, 20 parts of shield throat agent, 8 parts of penetrating agents, 5 parts of rush local blood circulation agent, 8 parts of lactose, 7 parts of D-sorbites, 20 parts of quality
16 mesh sieve whole grains are crossed in the hydroxypropyl methyl cellulose ethanol solution of fraction 6%, 4 parts of magnesium stearate mixing, spraying granulation, discharging,
Sieving particle is collected, tabletting, piece weight 0.55g, pressing plate is up to throat lozenge.
Comparative example 1
The activity extract A that wets one's whistle takes beggar-ticks in mass ratio 1：8 add in the methanol solution that mass fraction is 70%, and 32 DEG C extract 6d,
It filters, filtrate decompression is taken to concentrate, obtains concentrate, takes paste in mass ratio 1：10 add in water mixing, successively with petroleum ether, acetic acid
Ethyl ester, n-butanol respectively extract 3 times, combining extraction liquid, rotary evaporation, and must wet one's whistle activity extract A.
The activity extract B that wets one's whistle takes Wikstroemia indica stem skin, crushed 80 mesh sieves, collects sieving particle, and in mass ratio 1：10 add
Enter the ethyl alcohol that mass fraction is 95%, in 160W ultrasonic extraction 1.5h, filtering takes filtrate, rotary evaporation, the activity that must wet one's whistle extraction
Object B.
It is to take Heterophylly Falsesatarwort Root in mass ratio 1 to protect throat agent：9 add in distilled water mixing, extract 120 min in 95 DEG C, filter,
Filtrate is taken, concentrates, obtains concentrate, in mass ratio 1：8 add in the ethanol solution that mass fraction is 80%, stand 3h, take pellet frozen
It is dry, throat agent must be protected.
Penetrating agent is to take L-arginine in mass ratio 1：9 are dissolved in distilled water, take lysate in mass ratio 5：1：1 adds in
N-hydroxysuccinimide and 1-（3- dimethylamino-propyls）- 3- ethyl-carbodiimide hydrochlorides adjust pH to 6, anti-in 28 DEG C
3h is answered, obtains product, takes chitosan in mass ratio 2：3：10 add in product and mass fraction as 1% AcOH aqueous solutions, stirring
For 24 hours, distilled water dialysis 2d, filtering takes filtrate, is freeze-dried, obtains penetrating agent for mixing.
It is to take passion fruit in mass ratio 1 to promote local blood circulation agent：8 add in the ethyl alcohol that mass fraction is 95%, in 30
DEG C, 30W microwaves dip dyeing 1.5h, 8000r/min centrifugation 10min, take supernatant, be evaporated under reduced pressure, concentration, obtain rush local blood circulation
Agent.
A kind of preparation method of throat lozenge according to the mass fraction, takes 30 parts of activity extract A that wet one's whistle, 35 parts of activity that wet one's whistle
Extract B, 20 parts of shield throat agent, 8 parts of penetrating agents, 5 parts promote local blood circulation agent mixing, spraying granulation, and it is whole to cross 16 mesh sieves for discharging
Grain collects sieving particle, and tabletting, piece weight 0.55g, pressing plate is up to throat lozenge.
Comparative example 2
The activity extract A that wets one's whistle takes beggar-ticks in mass ratio 1：8 add in the methanol solution that mass fraction is 70%, and 32 DEG C extract 6d,
It filters, filtrate decompression is taken to concentrate, obtains concentrate, takes paste in mass ratio 1：10 add in water mixing, successively with petroleum ether, acetic acid
Ethyl ester, n-butanol respectively extract 3 times, combining extraction liquid, rotary evaporation, and must wet one's whistle activity extract A.
The activity extract B that wets one's whistle takes Wikstroemia indica stem skin, crushed 80 mesh sieves, collects sieving particle, and in mass ratio 1：10 add
Enter the ethyl alcohol that mass fraction is 95%, in 160W ultrasonic extraction 1.5h, filtering takes filtrate, rotary evaporation, the activity that must wet one's whistle extraction
Object B.
It is to take Heterophylly Falsesatarwort Root in mass ratio 1 to protect throat agent：9 add in distilled water mixing, extract 120 min in 95 DEG C, filter,
Filtrate is taken, concentrates, obtains concentrate, in mass ratio 1：8 add in the ethanol solution that mass fraction is 80%, stand 3h, take pellet frozen
It is dry, throat agent must be protected.
Penetrating agent is to take L-arginine in mass ratio 1：9 are dissolved in distilled water, take lysate in mass ratio 5：1：1 adds in
N-hydroxysuccinimide and 1-（3- dimethylamino-propyls）- 3- ethyl-carbodiimide hydrochlorides adjust pH to 6, anti-in 28 DEG C
3h is answered, obtains product, takes chitosan in mass ratio 2：3：10 add in product and mass fraction as 1% AcOH aqueous solutions, stirring
For 24 hours, distilled water dialysis 2d, filtering takes filtrate, is freeze-dried, obtains penetrating agent for mixing.
It is to take passion fruit in mass ratio 1 to promote local blood circulation agent：8 add in the ethyl alcohol that mass fraction is 95%, in 30
DEG C, 30W microwaves dip dyeing 1.5h, 8000r/min centrifugation 10min, take supernatant, be evaporated under reduced pressure, concentration, obtain rush local blood circulation
Agent.
A kind of preparation method of throat lozenge according to the mass fraction, takes 35 parts of activity extract B of wetting one's whistle, 20 parts of shield throat agent, and 8
Part penetrating agent, 5 parts of rush local blood circulation agent, 8 parts of lactose, 7 parts of D-sorbites, the hydroxypropyl methyl of 20 parts of mass fractions 6% are fine
The plain ethanol solution of dimension, 4 parts of magnesium stearate mixing, spraying granulation, discharging cross 16 mesh sieve whole grains, collect sieving particle, tabletting, piece
Weight 0.55g, pressing plate is up to throat lozenge.
Comparative example 3
The activity extract A that wets one's whistle takes beggar-ticks in mass ratio 1：8 add in the methanol solution that mass fraction is 70%, and 32 DEG C extract 6d,
It filters, filtrate decompression is taken to concentrate, obtains concentrate, takes paste in mass ratio 1：10 add in water mixing, successively with petroleum ether, acetic acid
Ethyl ester, n-butanol respectively extract 3 times, combining extraction liquid, rotary evaporation, and must wet one's whistle activity extract A.
The activity extract B that wets one's whistle takes Wikstroemia indica stem skin, crushed 80 mesh sieves, collects sieving particle, and in mass ratio 1：10 add
Enter the ethyl alcohol that mass fraction is 95%, in 160W ultrasonic extraction 1.5h, filtering takes filtrate, rotary evaporation, the activity that must wet one's whistle extraction
Object B.
It is to take Heterophylly Falsesatarwort Root in mass ratio 1 to protect throat agent：9 add in distilled water mixing, extract 120 min in 95 DEG C, filter,
Filtrate is taken, concentrates, obtains concentrate, in mass ratio 1：8 add in the ethanol solution that mass fraction is 80%, stand 3h, take pellet frozen
It is dry, throat agent must be protected.
Penetrating agent is to take L-arginine in mass ratio 1：9 are dissolved in distilled water, take lysate in mass ratio 5：1：1 adds in
N-hydroxysuccinimide and 1-（3- dimethylamino-propyls）- 3- ethyl-carbodiimide hydrochlorides adjust pH to 6, anti-in 28 DEG C
3h is answered, obtains product, takes chitosan in mass ratio 2：3：10 add in product and mass fraction as 1% AcOH aqueous solutions, stirring
For 24 hours, distilled water dialysis 2d, filtering takes filtrate, is freeze-dried, obtains penetrating agent for mixing.
It is to take passion fruit in mass ratio 1 to promote local blood circulation agent：8 add in the ethyl alcohol that mass fraction is 95%, in 30
DEG C, 30W microwaves dip dyeing 1.5h, 8000r/min centrifugation 10min, take supernatant, be evaporated under reduced pressure, concentration, obtain rush local blood circulation
Agent.
A kind of preparation method of throat lozenge according to the mass fraction, takes 30 parts of activity extract A of wetting one's whistle, 20 parts of shield throat agent, and 8
Part penetrating agent, 5 parts of rush local blood circulation agent, 8 parts of lactose, 7 parts of D-sorbites, the hydroxypropyl methyl of 20 parts of mass fractions 6% are fine
The plain ethanol solution of dimension, 4 parts of magnesium stearate mixing, spraying granulation, discharging cross 16 mesh sieve whole grains, collect sieving particle, tabletting, piece
Weight 0.55g, pressing plate is up to throat lozenge.
Comparative example 4
The activity extract A that wets one's whistle takes beggar-ticks in mass ratio 1：8 add in the methanol solution that mass fraction is 70%, and 32 DEG C extract 6d,
It filters, filtrate decompression is taken to concentrate, obtains concentrate, takes paste in mass ratio 1：10 add in water mixing, successively with petroleum ether, acetic acid
Ethyl ester, n-butanol respectively extract 3 times, combining extraction liquid, rotary evaporation, and must wet one's whistle activity extract A.
The activity extract B that wets one's whistle takes Wikstroemia indica stem skin, crushed 80 mesh sieves, collects sieving particle, and in mass ratio 1：10 add
Enter the ethyl alcohol that mass fraction is 95%, in 160W ultrasonic extraction 1.5h, filtering takes filtrate, rotary evaporation, the activity that must wet one's whistle extraction
Object B.
It is to take Heterophylly Falsesatarwort Root in mass ratio 1 to protect throat agent：9 add in distilled water mixing, extract 120 min in 95 DEG C, filter,
Filtrate is taken, concentrates, obtains concentrate, in mass ratio 1：8 add in the ethanol solution that mass fraction is 80%, stand 3h, take pellet frozen
It is dry, throat agent must be protected.
Penetrating agent is to take L-arginine in mass ratio 1：9 are dissolved in distilled water, take lysate in mass ratio 5：1：1 adds in
N-hydroxysuccinimide and 1-（3- dimethylamino-propyls）- 3- ethyl-carbodiimide hydrochlorides adjust pH to 6, anti-in 28 DEG C
3h is answered, obtains product, takes chitosan in mass ratio 2：3：10 add in product and mass fraction as 1% AcOH aqueous solutions, stirring
For 24 hours, distilled water dialysis 2d, filtering takes filtrate, is freeze-dried, obtains penetrating agent for mixing.
It is to take passion fruit in mass ratio 1 to promote local blood circulation agent：8 add in the ethyl alcohol that mass fraction is 95%, in 30
DEG C, 30W microwaves dip dyeing 1.5h, 8000r/min centrifugation 10min, take supernatant, be evaporated under reduced pressure, concentration, obtain rush local blood circulation
Agent.
A kind of preparation method of throat lozenge according to the mass fraction, takes 30 parts of activity extract A that wet one's whistle, 35 parts of activity that wet one's whistle
Extract B, 8 parts of penetrating agents, 5 parts of rush local blood circulation agent, 8 parts of lactose, 7 parts of D-sorbites, the hydroxyl of 20 parts of mass fractions 6%
Propyl methocel ethanol solution, 4 parts of magnesium stearate mixing, spraying granulation, discharging cross 16 mesh sieve whole grains, collect sieving
Grain, tabletting, piece weight 0.55g, pressing plate is up to throat lozenge.
Comparative example 5
The activity extract A that wets one's whistle takes beggar-ticks in mass ratio 1：8 add in the methanol solution that mass fraction is 70%, and 32 DEG C extract 6d,
It filters, filtrate decompression is taken to concentrate, obtains concentrate, takes paste in mass ratio 1：10 add in water mixing, successively with petroleum ether, acetic acid
Ethyl ester, n-butanol respectively extract 3 times, combining extraction liquid, rotary evaporation, and must wet one's whistle activity extract A.
The activity extract B that wets one's whistle takes Wikstroemia indica stem skin, crushed 80 mesh sieves, collects sieving particle, and in mass ratio 1：10 add
Enter the ethyl alcohol that mass fraction is 95%, in 160W ultrasonic extraction 1.5h, filtering takes filtrate, rotary evaporation, the activity that must wet one's whistle extraction
Object B.
It is to take Heterophylly Falsesatarwort Root in mass ratio 1 to protect throat agent：9 add in distilled water mixing, extract 120 min in 95 DEG C, filter,
Filtrate is taken, concentrates, obtains concentrate, in mass ratio 1：8 add in the ethanol solution that mass fraction is 80%, stand 3h, take pellet frozen
It is dry, throat agent must be protected.
Penetrating agent is to take L-arginine in mass ratio 1：9 are dissolved in distilled water, take lysate in mass ratio 5：1：1 adds in
N-hydroxysuccinimide and 1-（3- dimethylamino-propyls）- 3- ethyl-carbodiimide hydrochlorides adjust pH to 6, anti-in 28 DEG C
3h is answered, obtains product, takes chitosan in mass ratio 2：3：10 add in product and mass fraction as 1% AcOH aqueous solutions, stirring
For 24 hours, distilled water dialysis 2d, filtering takes filtrate, is freeze-dried, obtains penetrating agent for mixing.
It is to take passion fruit in mass ratio 1 to promote local blood circulation agent：8 add in the ethyl alcohol that mass fraction is 95%, in 30
DEG C, 30W microwaves dip dyeing 1.5h, 8000r/min centrifugation 10min, take supernatant, be evaporated under reduced pressure, concentration, obtain rush local blood circulation
Agent.
A kind of preparation method of throat lozenge according to the mass fraction, takes 30 parts of activity extract A that wet one's whistle, 35 parts of activity that wet one's whistle
Extract B, 20 parts of shield throat agent, 5 parts of rush local blood circulation agent, 8 parts of lactose, 7 parts of D-sorbites, the hydroxyl of 20 parts of mass fractions 6%
Propyl methocel ethanol solution, 4 parts of magnesium stearate mixing, spraying granulation, discharging cross 16 mesh sieve whole grains, collect sieving
Grain, tabletting, piece weight 0.55g, pressing plate is up to throat lozenge.
Comparative example 6
The activity extract A that wets one's whistle takes beggar-ticks in mass ratio 1：8 add in the methanol solution that mass fraction is 70%, and 32 DEG C extract 6d,
It filters, filtrate decompression is taken to concentrate, obtains concentrate, takes paste in mass ratio 1：10 add in water mixing, successively with petroleum ether, acetic acid
Ethyl ester, n-butanol respectively extract 3 times, combining extraction liquid, rotary evaporation, and must wet one's whistle activity extract A.
The activity extract B that wets one's whistle takes Wikstroemia indica stem skin, crushed 80 mesh sieves, collects sieving particle, and in mass ratio 1：10 add
Enter the ethyl alcohol that mass fraction is 95%, in 160W ultrasonic extraction 1.5h, filtering takes filtrate, rotary evaporation, the activity that must wet one's whistle extraction
Object B.
It is to take Heterophylly Falsesatarwort Root in mass ratio 1 to protect throat agent：9 add in distilled water mixing, extract 120 min in 95 DEG C, filter,
Filtrate is taken, concentrates, obtains concentrate, in mass ratio 1：8 add in the ethanol solution that mass fraction is 80%, stand 3h, take pellet frozen
It is dry, throat agent must be protected.
Penetrating agent is to take L-arginine in mass ratio 1：9 are dissolved in distilled water, take lysate in mass ratio 5：1：1 adds in
N-hydroxysuccinimide and 1-（3- dimethylamino-propyls）- 3- ethyl-carbodiimide hydrochlorides adjust pH to 6, anti-in 28 DEG C
3h is answered, obtains product, takes chitosan in mass ratio 2：3：10 add in product and mass fraction as 1% AcOH aqueous solutions, stirring
For 24 hours, distilled water dialysis 2d, filtering takes filtrate, is freeze-dried, obtains penetrating agent for mixing.
It is to take passion fruit in mass ratio 1 to promote local blood circulation agent：8 add in the ethyl alcohol that mass fraction is 95%, in 30
DEG C, 30W microwaves dip dyeing 1.5h, 8000r/min centrifugation 10min, take supernatant, be evaporated under reduced pressure, concentration, obtain rush local blood circulation
Agent.
A kind of preparation method of throat lozenge according to the mass fraction, takes 30 parts of activity extract A that wet one's whistle, 35 parts of activity that wet one's whistle
Extract B, 20 parts of shield throat agent, 8 parts of penetrating agents, 8 parts of lactose, 7 parts of D-sorbites, the hydroxypropyl methyl of 20 parts of mass fractions 6% are fine
The plain ethanol solution of dimension, 4 parts of magnesium stearate mixing, spraying granulation, discharging cross 16 mesh sieve whole grains, collect sieving particle, tabletting, piece
Weight 0.55g, pressing plate is up to throat lozenge.
Comparative example 7：The throat lozenge of Bozhou pharmaceutcal corporation, Ltd production
Method：Selection 120 has the volunteer that pharyngitis perplexs, and is divided into 10 groups, and 3 times a day, 1 tablet once, takes lasting 1 month,
Detect the effect of its throat lozenge treatment pharyngitis.
The specific detection case of throat lozenge such as table 1
Table 1
| Detection project | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Comparative example 2 | Comparative example 3 | Comparative example 4 | Comparative example 5 | Comparative example 6 | Comparative example 7 |
| Recovery from illness | 5 | 6 | 7 | 4 | 5 | 4 | 3 | 4 | 4 | 2 |
| It improves | 4 | 4 | 3 | 4 | 3 | 3 | 3 | 3 | 2 | 2 |
| It is invalid | 1 | 0 | 0 | 2 | 1 | 3 | 4 | 3 | 4 | 6 |
Recovery from illness：It without sense of discomfort, does not swell and ache, no phlegm, the illness of cough occur.
It improves：Relatively comfortable, few phlegm, slight swelling and pain, few status of cough occur.
It is invalid：Abscess of throat, cough, more phlegm.
From the foregoing, it will be observed that each component contained by throat lozenge prepared by the present invention has synergistic effect, it can be effective
The problem of alleviating pharyngitis, and possesses the effect of good clearing heat and detoxicating, is a kind of safe and efficient throat lozenge, be worthy to be popularized and
It uses.
Claims (7)
1. a kind of throat lozenge, according to the mass fraction, including 7 ~ 8 parts of lactose, 4 ~ 7 parts of D-sorbites, 15 ~ 20 parts of mass fractions 6%
Hydroxypropyl methyl cellulose ethanol solution, 2 ~ 4 parts of magnesium stearates, which is characterized in that further include 20 ~ 30 parts of activity extracts that wet one's whistle
A, 25 ~ 35 parts of activity extract B that wet one's whistle, 15 ~ 20 parts of shield throat agent, 6 ~ 8 parts of penetrating agents, 3 ~ 5 parts of rush local blood circulation agent.
2. throat lozenge according to claim 1, which is characterized in that the activity extract A that wets one's whistle takes beggar-ticks by quality
Than 1：8 add in the methanol solution that mass fraction is 70%, and 25 ~ 32 DEG C of 5 ~ 6d of extraction are filtered, filtrate decompression is taken to concentrate, must be concentrated
Object takes paste in mass ratio 1：10 add in water mixing, are respectively extracted 3 times, merged with petroleum ether, ethyl acetate, n-butanol successively
Extract liquor, rotary evaporation, must wet one's whistle activity extract A.
3. throat lozenge according to claim 1, which is characterized in that the activity extract B that wets one's whistle takes Wikstroemia indica stem skin, powder
Broken 80 mesh sieve of mistake, collection sieving particle, in mass ratio 1：10 add in the ethyl alcohol that mass fraction is 95%, are carried in 140 ~ 160W ultrasounds
1 ~ 1.5h is taken, filters, takes filtrate, rotary evaporation, must wet one's whistle activity extract B.
4. throat lozenge according to claim 1, which is characterized in that the shield throat agent is to take Heterophylly Falsesatarwort Root in mass ratio 1：
9 add in distilled water mixing, and 110 ~ 120 min are extracted in 90 ~ 95 DEG C, and filtering takes filtrate, concentrates, obtains concentrate, in mass ratio 1：
8 add in the ethanol solution that mass fraction is 80%, stand 2 ~ 3h, pellet frozen is taken to dry, must protect throat agent.
5. throat lozenge according to claim 1, which is characterized in that the penetrating agent is to take L-arginine in mass ratio 1：9
It is dissolved in distilled water, takes lysate in mass ratio 5：1：1 add in n-hydroxysuccinimide and 1- (3- dimethylamino-propyls)-
3- ethyl-carbodiimide hydrochlorides adjust pH to 6, react 2 ~ 3h in 25 ~ 28 DEG C, obtain product, take chitosan in mass ratio 2：
3：10 to add in products and mass fraction be 1% AcOH aqueous solutions, is stirred 18 ~ for 24 hours, and distilled water is dialysed 1.5 ~ 2d, filtering,
Filtrate is taken, is freeze-dried, obtains penetrating agent.
6. throat lozenge according to claim 1, which is characterized in that the rush local blood circulation agent is to take passion fruit by matter
Measure ratio 1：8 add in the ethyl alcohol that mass fraction is 95%, disseminate 1 ~ 1.5h, 8000r/min centrifugations in 25 ~ 30 DEG C, 30W microwaves
10min takes supernatant, is evaporated under reduced pressure, and concentration must promote local blood circulation agent.
7. a kind of preparation method of throat lozenge as described in claim 1, which is characterized in that the preparation method includes the following steps：
The activity extract A that wets one's whistle is taken, wet one's whistle activity extract B, protects throat agent, and penetrating agent promotees local blood circulation agent, lactose, sorbose
It is whole to cross 16 mesh sieves for alcohol, the hydroxypropyl methyl cellulose ethanol solution of mass fraction 6%, magnesium stearate mixing, spraying granulation, discharging
Grain collects sieving particle, tabletting, and piece weighs 0.5 ~ 0.55g, and pressing plate is to get throat lozenge.
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Application publication date: 20180605 |