CN108067147A - A kind of liquid mixer and its application - Google Patents
A kind of liquid mixer and its application Download PDFInfo
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- CN108067147A CN108067147A CN201711489851.7A CN201711489851A CN108067147A CN 108067147 A CN108067147 A CN 108067147A CN 201711489851 A CN201711489851 A CN 201711489851A CN 108067147 A CN108067147 A CN 108067147A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/40—Mixers using gas or liquid agitation, e.g. with air supply tubes
- B01F33/406—Mixers using gas or liquid agitation, e.g. with air supply tubes in receptacles with gas supply only at the bottom
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/71—Feed mechanisms
- B01F35/717—Feed mechanisms characterised by the means for feeding the components to the mixer
- B01F35/7174—Feed mechanisms characterised by the means for feeding the components to the mixer using pistons, plungers or syringes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
- B01F2101/44—Mixing of ingredients for microbiology, enzymology, in vitro culture or genetic manipulation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
- C12N2740/15041—Use of virus, viral particle or viral elements as a vector
- C12N2740/15043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
- C12N2740/15051—Methods of production or purification of viral material
- C12N2740/15052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
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- Organic Chemistry (AREA)
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Abstract
The present invention provides a kind of liquid mixer and its application, the liquid mixer is including being sequentially connected such as lower component：Propulsion device, container for mixing liquid (5) and feeder；Wherein, it is connected in each component by pipeline, the feeder includes aseptic air filter (7), for being passed through filtrated air into container for mixing liquid (5).The liquid mixer of the present invention is provided with liquor charging access and supply access, be it is a kind of conveniently, safely, efficient automation equipment；Slow virus packaging is carried out using the liquid mixer, process automation compared with artificial slow virus packing technique, improves packaging speed, reduces error, avoids pollution problem, is packed available for extensive slow virus.
Description
Technical field
The invention belongs to machinery equipment field and Medical Biology technical field, are related to a kind of liquid mixer and its answer
With.
Background technology
Transgenic technology is the most frequently used in molecular biology and most important technological means, and the purpose is to utilize biology
Method by the genome of manually modified gene into cells, so as to obtain the cell with specific inhereditary feature.
In recent years, slow virus has become most important nucleic acid delivery instrument, in tissue cultures, structure animal model and clinic
It is had a wide range of applications in treatment.Slow virus system can make the genome of target cell obtain the nucleotide sequence for stablizing heredity,
So that gene construct (such as siRNA or albumen coded sequence) is permanently expressed in cell.Slow virus has infection nondividing
Phase cell accommodates large fragment allogenic gene, does not generate the remarkable advantages such as immune response, is a kind of powerful outer-gene transport
Instrument.
The packing method of slow virus mainly includes lipofection and calcium phosphate transfection method.Wherein, lipofection
Cost is higher, is not suitable for mass producing；Calcium phosphate transfection method cost is relatively low, but to the mixing side of plasmid and transfection reagent
Formula and operating technology have higher requirement, at this stage mostly manual operation, and not only error is larger, and it is existing to be easy to cause pollution
As.
Therefore it provides a kind of packing method of automation, is of great significance in slow virus field.
The content of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of body mixer and its application, to realize the automatic of slow virus
Change packaging.
For this purpose, the present invention uses following technical scheme：
In a first aspect, the present invention provides a kind of liquid mixer, including being sequentially connected such as lower component：Propulsion device,
Container for mixing liquid 5 and feeder；
Wherein, it is connected in each component by pipeline, the feeder includes aseptic air filter 7, for liquid
Filtrated air is passed through in body mixing vessel 5.
The liquid mixer of the present invention by setting propulsion device and feeder, formed one kind can constant speed quantitative give
Sample but can constant speed quantitative supplies into liquid automation equipment, be it is a kind of conveniently, safely, efficient automation equipment, bubble
The mixing velocity for improving liquid is burst in a liquid, solute particle is made to form the structure of particular size, and liquid evenly, operates
It is simpler.
Preferably, the propulsion device includes propeller 1, syringe 2, sample presentation pipeline 3 and dropping liquid bucket 4.
Preferably, the syringe 2 is loaded on propeller 1.
Preferably, the syringe 2 is connected by sample presentation pipeline 3 with dropping liquid bucket 4.
Preferably, the dropping liquid bucket 4 is fixed on the mixing pedestal of container for mixing liquid 5.
Preferably, liquid to be mixed is housed in the dropping liquid bucket 4.
Preferably, the feeder includes air feed pump 8, aseptic air filter 7 and steam line 6.
Preferably, the air feed pump 8 is connected by steam line 6 with container for mixing liquid 5.
Preferably, aseptic air filter 7 is housed on the steam line 6.
Preferably, the air feed pump 8 is passed through aseptic gas by steam line 6 into container for mixing liquid 5.
Preferably, the volume of the dropping liquid bucket 4 be 10-50mL, such as can be 10mL, 20mL, 30mL, 40mL or
50mL。
Preferably, the volume of the container for mixing liquid 5 be 1-100mL, such as can be 1mL, 10mL, 20mL, 30mL,
40mL, 50mL, 60mL, 70mL, 80mL, 90mL or 100mL.
Preferably, the aperture of the aseptic air filter 7 is 5-20 μm, such as can be 5 μm, 6 μm, 7 μm, 8 μm, 9 μ
M, 10 μm, 11 μm, 12 μm, 13 μm, 14 μm, 15 μm, 16 μm, 17 μm, 18 μm, 19 μm or 20 μm.
In the present invention, the course of work of the liquid mixer is as follows：
After 1 loading injector 2 of propeller, it is connected to by sample presentation pipeline 3 on dropping liquid bucket 4, dropping liquid bucket 4 is threadably secured
On the mixing pedestal of container for mixing liquid 5, liquor charging access is formed；Air feed pump 8 is by being loaded with the confession of aseptic air filter 7
Feed channel 6 is connected to the blowhole of the mixing pedestal of container for mixing liquid 5, forms supply access；The specific work of liquid mixer
It is as process：Solution A is added in container for mixing liquid 5, solution B is added in dropping liquid bucket 4；Air feed pump 8 is opened, gas is through nothing
After bacterium air filter 7 filters, by steam line 6, the air intake that base bottom is mixed through container for mixing liquid 5 is delivered to liquid
In body mixing vessel 5；Propeller 1 is opened, the gas in syringe 2, into dropping liquid bucket 4, squeezes dropping liquid bucket 4 through sample presentation pipeline 3
In liquid B be added dropwise to lower section container for mixing liquid 5 in, be sufficiently mixed with solution A.
Second aspect, the present invention provides a kind of liquid mixer as described in relation to the first aspect answering in slow virus is packed
With.
The third aspect, the present invention provides a kind of methods for packing slow virus, comprise the following steps：
1) balanced salt solution is added in container for mixing liquid 5, by slow virus carrier and CaCl2Mixed solution add in drop
In liquid bucket 4；
2) air feed pump 8 is opened, gas, by steam line 6, is mixed through liquid and held after the filtering of aseptic air filter 7
The air intake that device 5 mixes base bottom is delivered in container for mixing liquid 5；
3) propeller 1 is opened, the gas in syringe 2, into dropping liquid bucket 4, is squeezed in dropping liquid bucket 4 through sample presentation pipeline 3
Mixed liquor is added dropwise in the container for mixing liquid 5 of lower section, is sufficiently mixed with balanced salt solution, is obtained slow-virus transfection reagent；
4) the slow-virus transfection reagent described in step 3) is dropped evenly in cell culture fluid, carries out slow-virus transfection.
In the present invention, the mixed of slow-virus transfection reagent is carried out using liquid mixer automation as described in relation to the first aspect
Close, for slow virus pack, improve efficiency, saved cost, reduce error, avoid pollution problem, be it is a kind of safety,
Convenient, efficient automatic mode, is packed available for extensive slow virus.
In the present invention, the balanced salt solution, mainly by inorganic salts (phosphate, sodium chloride, potassium chloride) and glucose group
Into for Premeabilisation of cells pressure to be maintained to balance, holding pH is stable and provides simple nutrition.
Preferably, the supply speed of the step 2) air feed pump 8 be 1-100mL/min, such as can be 1mL/min,
10mL/min、20mL/min、30mL/min、40mL/min、50mL/min、60mL/min、70mL/min、80mL/min、90mL/
Min or 100mL/min is preferably 10-50mL/min.
Preferably, the fltting speed of the step 3) propeller 1 is 0.1-5000 μ L/min, such as can be 0.1 μ L/
Min, 1 μ L/min, 100 μ L/min, 500 μ L/min, 1000 μ L/min, 2000 μ L/min, 3000 μ L/min, 4000 μ L/min or
5000 μ L/min are preferably 10-1000 μ L/min.
As optimal technical scheme, the present invention provides a kind of methods for packing slow virus, comprise the following steps：
1) balanced salt solution is added in container for mixing liquid 5, by slow virus carrier and CaCl2Mixed solution add in drop
In liquid bucket 4；
2) air feed pump 8 is opened, supply speed is 1-100mL/min, and gas passes through after the filtering of aseptic air filter 7
Steam line 6, the air intake that base bottom is mixed through container for mixing liquid 5 are delivered in container for mixing liquid 5；
3) propeller 1 is opened, fltting speed is 0.1-5000 μ L/min, and the gas in syringe 2 enters through sample presentation pipeline 3
In dropping liquid bucket 4, squeeze the mixed liquor in dropping liquid bucket 4 and be added dropwise in the container for mixing liquid 5 of lower section, it is fully mixed with balanced salt solution
It closes, obtains slow-virus transfection reagent；
4) the slow-virus transfection reagent described in step 3) is dropped evenly in cell culture fluid, carries out slow-virus transfection.
Compared with prior art, the present invention has the advantages that：
(1) for liquid mixer of the invention by setting liquor charging access and supply access, forming one kind can constant speed quantitative
To sample but can constant speed quantitative supplies into liquid automation equipment, improve the mixing velocity of liquid, liquid evenly, behaviour
Make it is simpler, be it is a kind of conveniently, safely, efficient automation equipment；
(2) slow virus packaging, process automation, with artificial slow virus packing technique phase are carried out using the liquid mixer
Than improving packaging speed, reducing error, avoid pollution problem, packed available for extensive slow virus.
Description of the drawings
Fig. 1 is the structure diagram of liquid mixer provided by the invention, wherein, 1- propellers, 2- syringes, 3- sample presentations
Pipeline, 4- dropping liquid buckets, 5- container for mixing liquid, 6- steam lines, 7- aseptic air filters, 8- air feed pumps.
Specific embodiment
The technological means and its effect taken for the present invention is further explained, with reference to embodiments with attached drawing to this hair
It is bright to be further described.It is understood that the specific embodiments described herein be used only for explain the present invention rather than
Limitation of the invention.
In the examples where no specific technique or condition is specified, according to the described technology of document in the art or condition,
Or it is carried out according to product description.Reagents or instruments used without specified manufacturer, be can be by regular channel commercially available from
The conventional products of acquisition.
Embodiment 1
The structure diagram of the liquid mixer of the present embodiment is as shown in Figure 1.
The liquid mixer is including being sequentially connected such as lower unit：Propulsion device, container for mixing liquid 5 and supply dress
It puts；Wherein, it is connected in each component by pipeline, the feeder includes aseptic air filter 7, for being mixed to liquid
Filtrated air is passed through in container 5；
The propulsion device includes propeller 1, syringe 2, sample presentation pipeline 3 and dropping liquid bucket 4；
The syringe 2 is loaded on propeller 1；
The syringe 2 is connected by sample presentation pipeline 3 with dropping liquid bucket 4；
The dropping liquid bucket 4 is fixed on the mixing pedestal of container for mixing liquid 5；
Liquid to be mixed is housed in the dropping liquid bucket 4；
The feeder includes air feed pump 8, aseptic air filter 7 and steam line 6；
The air feed pump 8 is connected by steam line 6 with container for mixing liquid 5；
Aseptic air filter 7 is housed on the steam line 6；
The air feed pump 8 is passed through aseptic gas by steam line 6 into container for mixing liquid 5；
The volume of the dropping liquid bucket 4 is 10-50mL；
The volume of the container for mixing liquid 5 is 1-100mL；
The aperture of the aseptic air filter 7 is 5-20 μm.
The course of work of the liquid mixer is：
Solution A is added in container for mixing liquid 5, solution B is added in dropping liquid bucket 4；Air feed pump 8 is opened, gas is through nothing
After bacterium air filter 7 filters, by steam line 6, the air intake that base bottom is mixed through container for mixing liquid 5 is delivered to liquid
In body mixing vessel 5；Propeller 1 is opened, the gas in syringe 2, into dropping liquid bucket 4, squeezes dropping liquid bucket 4 through sample presentation pipeline 3
In solution B be added dropwise to lower section container for mixing liquid 5 in, be sufficiently mixed with solution A.
Embodiment 2
The present embodiment carries out slow virus packaging using liquid mixer as described in Example 1, concretely comprises the following steps：
1) balanced salt solution is added in container for mixing liquid 5, by slow virus carrier and CaCl2Mixed solution add in drop
In liquid bucket 4；
2) air feed pump 8 is opened, supply speed is 1mL/min, and gas passes through supply after the filtering of aseptic air filter 7
Pipeline 6, the air intake that base bottom is mixed through container for mixing liquid 5 are delivered in container for mixing liquid 5；
3) propeller 1 is opened, fltting speed is 0.1 μ L/min, and the gas in syringe 2 enters dropping liquid through sample presentation pipeline 3
In bucket 4, squeeze the mixed liquor in dropping liquid bucket 4 and be added dropwise in the container for mixing liquid 5 of lower section, be sufficiently mixed with balanced salt solution,
Obtain slow-virus transfection reagent；
4) the slow-virus transfection reagent described in step 3) is dropped evenly in cell culture fluid, carries out slow-virus transfection.
Embodiment 3
The present embodiment carries out slow virus packaging using liquid mixer as described in Example 1, concretely comprises the following steps：
1) balanced salt solution is added in container for mixing liquid 5, by slow virus carrier and CaCl2Mixed solution add in drop
In liquid bucket 4；
2) air feed pump 8 is opened, supply speed is 100mL/min, and gas passes through confession after the filtering of aseptic air filter 7
Feed channel 6, the air intake that base bottom is mixed through container for mixing liquid 5 are delivered in container for mixing liquid 5；
3) propeller 1 is opened, fltting speed is 5000 μ L/min, and the gas in syringe 2 enters dropping liquid through sample presentation pipeline 3
In bucket 4, squeeze the mixed liquor in dropping liquid bucket 4 and be added dropwise in the container for mixing liquid 5 of lower section, be sufficiently mixed with balanced salt solution,
Obtain slow-virus transfection reagent；
4) the slow-virus transfection reagent described in step 3) is dropped evenly in cell culture fluid, carries out slow-virus transfection.
The slow virus packaging process carries out the mixing of transfection reagent using automation equipment, for packing slow virus, with
Artificial technology compares, and the infect efficiency of two kinds of slow virus is basically identical, and automatic fluid mixer of the invention improves packaging
Speed reduces error, avoids pollution problem, is packed suitable for extensive slow virus.
In conclusion the liquid mixer of the present invention, by setting liquor charging access and supply access, forming one kind can determine
Speed it is quantitative to sample and can constant speed quantitative supplies into liquid automation equipment, improve the mixing velocity of liquid, liquid is more equal
Even, operation is simpler, be it is a kind of conveniently, safely, efficient automation equipment；Slow virus bag is carried out using the liquid mixer
Dress, process automation compared with artificial slow virus packing technique, improve packaging speed, reduce error, avoid pollution and ask
Topic is packed available for extensive slow virus.
Applicant states that the present invention illustrates the method detailed of the present invention, but not office of the invention by above-described embodiment
It is limited to above-mentioned method detailed, that is, does not mean that the present invention has to rely on above-mentioned method detailed and could implement.Technical field
Technical staff it will be clearly understood that any improvement in the present invention, equivalence replacement and auxiliary element to each raw material of product of the present invention
Addition, selection of concrete mode etc., all fall within protection scope of the present invention and the open scope.
Claims (10)
1. a kind of liquid mixer, which is characterized in that including being sequentially connected such as lower component：Propulsion device, container for mixing liquid
(5) and feeder；
Wherein, it is connected in each component by pipeline, the feeder includes aseptic air filter (7), for liquid
Mixing vessel is passed through filtrated air in (5).
2. liquid mixer according to claim 1, which is characterized in that the propulsion device includes propeller (1), injection
Device (2), sample presentation pipeline (3) and dropping liquid bucket (4)；
Preferably, the syringe (2) is loaded on propeller (1)；
Preferably, the syringe (2) is connected by sample presentation pipeline (3) with dropping liquid bucket (4)；
Preferably, the dropping liquid bucket (4) is fixed on the mixing pedestal of container for mixing liquid (5)；
Preferably, liquid to be mixed is housed in the dropping liquid bucket (4).
3. liquid mixer according to claim 1 or 2, which is characterized in that the feeder include air feed pump (8),
Aseptic air filter (7) and steam line (6)；
Preferably, the air feed pump (8) is connected by steam line (6) with container for mixing liquid (5)；
Preferably, aseptic air filter (7) is housed on the steam line (6)；
Preferably, the air feed pump (8) is passed through aseptic gas by steam line (6) into container for mixing liquid (5).
4. according to claim 1-3 any one of them liquid mixers, which is characterized in that the volume of the dropping liquid bucket (4) is
10-50mL；
Preferably, the volume of the container for mixing liquid (5) is 1-100mL.
5. according to claim 1-4 any one of them liquid mixers, which is characterized in that the aseptic air filter (7)
Aperture be 5-20 μm.
It is 6. a kind of such as application of the claim 1-5 any one of them liquid mixer in slow virus is packed.
A kind of 7. method for packing slow virus, which is characterized in that comprise the following steps：
1) balanced salt solution is added in container for mixing liquid (5), by slow virus carrier and CaCl2Mixed solution add in dropping liquid
In bucket (4)；
2) air feed pump (8) is opened, gas, by steam line (6), mixes after aseptic air filter (7) filtering through liquid
The air intake of container (5) mixing base bottom is delivered in container for mixing liquid (5)；
3) propeller (1) is opened, the gas in syringe (2) into dropping liquid bucket (4), squeezes dropping liquid bucket through sample presentation pipeline (3)
(4) mixed liquor in is added dropwise in the container for mixing liquid (5) of lower section, is sufficiently mixed with balanced salt solution, is obtained slow virus and is turned
Transfection reagent；
4) the slow-virus transfection reagent described in step 3) is dropped evenly in cell culture fluid, carries out slow-virus transfection.
8. the method according to the description of claim 7 is characterized in that the supply speed of the step 2) air feed pump (8) is 1-
100mL/min is preferably 10-50mL/min.
9. according to 7 or 8 any one of them method of claim, which is characterized in that the propulsion speed of the step 3) propeller (1)
It spends for 0.1-5000 μ L/min, preferably 10-1000 μ L/min.
10. according to claim 7-9 any one of them methods, which is characterized in that comprise the following steps：
1) balanced salt solution is added in container for mixing liquid (5), by slow virus carrier and CaCl2Mixed solution add in dropping liquid
In bucket (4)；
2) air feed pump (8) is opened, supply speed is 1-100mL/min, and gas passes through after aseptic air filter (7) filtering
Steam line (6), the air intake through container for mixing liquid (5) mixing base bottom are delivered in container for mixing liquid (5)；
3) open propeller (1), fltting speed is 0.1-5000 μ L/min, the gas in syringe (2) through sample presentation pipeline (3) into
Enter in dropping liquid bucket (4), squeeze the mixed liquor in dropping liquid bucket (4) and be added dropwise in the container for mixing liquid (5) of lower section, it is molten with balance salt
Liquid is sufficiently mixed, and obtains slow-virus transfection reagent；
4) the slow-virus transfection reagent described in step 3) is dropped evenly in cell culture fluid, carries out slow-virus transfection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711489851.7A CN108067147B (en) | 2017-12-29 | 2017-12-29 | Liquid mixer and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711489851.7A CN108067147B (en) | 2017-12-29 | 2017-12-29 | Liquid mixer and application thereof |
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| CN108067147A true CN108067147A (en) | 2018-05-25 |
| CN108067147B CN108067147B (en) | 2021-12-03 |
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2017
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| CN101172176A (en) * | 2007-11-06 | 2008-05-07 | 刘天山 | Safe transfusion device and use method |
| CN201250225Y (en) * | 2008-05-16 | 2009-06-03 | 江苏省农业科学院 | Bacteria-free device for preparing microencapsulated cells |
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| CN108067147B (en) | 2021-12-03 |
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