CN108014202A - A kind of traditional Chinese medicine extract composition with anti-skin irritatin effect and preparation method thereof - Google Patents

A kind of traditional Chinese medicine extract composition with anti-skin irritatin effect and preparation method thereof Download PDF

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CN108014202A
CN108014202A CN201610937313.9A CN201610937313A CN108014202A CN 108014202 A CN108014202 A CN 108014202A CN 201610937313 A CN201610937313 A CN 201610937313A CN 108014202 A CN108014202 A CN 108014202A
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chinese medicine
traditional chinese
medicine extract
extract composition
effect
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林梦感
周利丹
卢伊娜
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Shanghai Jia Kai Biological Technology Co Ltd
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Shanghai Jia Kai Biological Technology Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/76—Salicaceae (Willow family), e.g. poplar
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/232—Angelica
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/236—Ligusticum (licorice-root)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/238—Saposhnikovia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/484—Glycyrrhiza (licorice)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/489—Sophora, e.g. necklacepod or mamani
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A61K36/538—Schizonepeta
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A61K36/539—Scutellaria (skullcap)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/55—Liquid-liquid separation; Phase separation

Abstract

The invention discloses a kind of traditional Chinese medicine extract composition with anti-skin irritatin effect and preparation method thereof, wherein formula includes radix glycyrrhizae 1 20%, kuh-seng 1 20%, radix scutellariae 1 20%, Angelica sinensis 1 20%, Ligusticum wallichii 1 20%, schizonepeta 1 20%, windproof 1 20%, salicis,cortex 1 20%, preparation method includes, S10, prepares the ethanol extract of Chinese medicine；S20, concentrates after ethanol extract filtering, purifies, eluent is collected after elution；S30, concentrate eluant, adds 1,3 butanediols, collects supernatant；S40, obtains centrifuged supernatant.The composition of the present invention can releive sensitive skin to extraneous stimulation from multipath, and contained active principle is all the extract of pure natural plant, without steroids, antihistaminic etc., so as to avoid stimulation of the chemicals to skin and sensitization.

Description

A kind of traditional Chinese medicine extract composition with anti-skin irritatin effect and preparation method thereof
Technical field
The invention belongs to Skin Soothing Agent field, and in particular to a kind of Chinese medical extract group with anti-skin irritatin effect Compound and preparation method thereof.
Background technology
Sensitive is a kind of extremely sensitive skin condition reduced to extraneous stimulant, is vulnerable to various factors Stimulate and produce shouting pain, burn, is tight, the multiple-factor syndrome of the subjective symptom such as itch, not only may alternatively appear in some diseases Preclinical phase or clinical phase, it may also appear in appearance and complete normal skin.
In recent years, with the change of dietary structure, atmosphere pollution, the increase of live and work pressure, sensitive skin crowd Sharply increase.Especially in developed countries such as Japan, the U.S., skin sensitivity has become the social concern for influencing human health. According to incompletely statistics, the whole world feel sensitiveness skin crowd it is more and more, wherein male accounts for 38%. women and accounts for 61%.
Sensitive is releived and resisted to extraneous stimulation, is all very heavy from skin physiology and medicine and beauty angle The problem of wanting.At present, Skin Soothing Agent declares that skin barrier can be repaired mostly, also has some products for sensitive Abnormal response（Including redness, itch, tingling sensation, tight feeling etc.）, also declare with anti-inflammatory or anti-allergic effects.Meanwhile have There is the severely afflicated area that this kind of cosmetic product declared is also the illegal additive such as hormone.The drug therapies such as steroids, antihistaminic Although it can make to be eased and treat with symptom the problem of sensitive within the regular hour, if using not When, it is likely that the state of an illness of skin is aggravated, or even the irremediable lesion of skin can be caused.Therefore, for how safely and effectively It is very worth research, it is necessary to which the integrated use of multipath preferably to solve skin irritatin to ask to suppress skin allergy problem Topic.
Therefore for problems of the prior art, it is really necessary to be studied, there is anti-skin irritatin to provide one kind Traditional Chinese medicine extract composition of effect and preparation method thereof, so as to avoid stimulation and sensitization of the chemicals to skin.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of traditional Chinese medicine extract composition with anti-skin irritatin effect And preparation method thereof.
In order to solve the above technical problems, the present invention adopts the following technical scheme that：
The present invention a kind of traditional Chinese medicine extract composition with anti-skin irritatin effect, comprising radix glycyrrhizae, kuh-seng, radix scutellariae, Angelica sinensis, Ligusticum wallichii, schizonepeta, windproof and salicis,cortex extract.
Preferably, based on the percentage by weight of medicinal material dry weight, comprising radix glycyrrhizae 1-20%, kuh-seng 1-20%, radix scutellariae 1-20%, when Return 1-20%, Ligusticum wallichii 1-20%, schizonepeta 1-20%, windproof 1-20%, salicis,cortex 1-20%.
A kind of preparation method of the traditional Chinese medicine extract composition with anti-skin irritatin effect, is used to prepare above-mentioned with anti- The traditional Chinese medicine extract composition of skin irritatin effect, specifically includes following steps：
S10, prepares radix glycyrrhizae, kuh-seng, radix scutellariae, Angelica sinensis, Ligusticum wallichii, schizonepeta, windproof and salicis,cortex ethanol extract；
S20, concentrates after ethanol extract filtering, is purified with macroporous absorbent resin, eluent is collected after elution；
S30, concentrate eluant, adds 1,3-BDO, stirs evenly, and when standing 24 is small, collects supernatant；
S40, is centrifuged supernatant, obtains centrifuged supernatant, being included in centrifuged supernatant has anti-skin irritatin work( The traditional Chinese medicine extract composition of effect.
Preferably, S10 specifically includes following steps：
S101, in grams, measures 1-20 parts of radix glycyrrhizae, 1-20 parts of kuh-seng, 1-20 parts of radix scutellariae, 1-20 parts of Angelica sinensis, Ligusticum wallichii 1-20 Part, 1-20 parts of schizonepeta, 1-20 parts windproof, 1-20 parts of salicis,cortex, note gross weight are numerical value A, and note gross weight is numerical value A；
S102, in units of milliliter, the volume for remembering ethanol water is numerical value B, and measuring the ethanol water of 20%-80% makes total Value B is 5-20 times of numerical value A；
S103, Chinese medicine soak extraction in the ethanol water of 20%-80%, extraction time are 1-3 times, merge ethanol extract.
Preferably, in S103, when the time of soak extraction is 2-48 small each time.
Preferably, in S20, ethanol extract concentrates 10-50 times.
Preferably, in S20, ethanol water that elution step is 10-95% with eluant, eluent.
Preferably, in S30, eluent concentrates 10-20 times.
Preferably, in S30, the additive amount of 1,3-BDO is 0.5-3 times of the volume of eluent.
There is following beneficial effect using the present invention：
（1）Licorice has anti-inflammatory effect, and licoflavone and glycyrrhizic acid therein can be respectively by improving anti-inflammatory factors The expression quantity of IL-10, reduce protective effect of the expression performance of inflammatory factor TNF-α to the UVB HaCaT cellular damages induced.
（2）Kuh-seng has effects that removing dampness to stop itchin, there is obvious curative effects, alkaloids therein to various cutaneous pruritus Compound, which can dramatically increase Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2, stimulates the secretory volume of HaCaT cell IFN-γ.
（3）Radix scutellariae has effects that heat-clearing and damp-drying drug, and flavonoids therein has stronger antioxidation activity, to various cells There is protective effect with tissue damage, can also block arachidonic acid path from different links, suppress secretion, the release of cell factor With the transcriptional activity of nuclear factor, suppress the synthesis of nitric oxide and some adhesion molecules.
（4）The chemical composition of Angelica sinensis mainly has phthalide-type and its dimer, phenolic acid class, polysaccharide, flavones etc..Angelica extract With anti-inflammatory and analgesic effect, and the mechanism of its anti-inflammatory, analgesic activity is related with suppressing Cox-2 mRNA and protein expression.
（5）Ligusticum wallichii can promoting blood circulation and removing blood stasis and promoting qi circulation and relieving pain, containing volatile oil, alkaloid, organic acid and polysaccharide etc. into Point.Ligustrazine has calcium antagonism, reduces capillary permeability, suppresses platelet aggregation, and promote prostacyclin I2 With the balance of thromboxane A2.
（6）Schizonepeta, it is windproof be in diaphoretic medicine common one group of medicine pair, schizonepeta and Radix Saposhnikoviae extract to acute inflammation, chronic Inflammation and allergic inflammation have significant inhibitory action, can reduce the PGE_2 contents of inflammation model, and can suppress scorching Property tissue in histamine and serotonin, suppress blood plasma in NO, total protein content and antalzyme activity.In windproof it is effective into Macrotin glycosides is divided can also effectively to suppress the synthesis of TNF-α, IL-1 β and IL-6 of the RAW264.7 cells of LPS inductions, rise The synthesis of IL-10, can suppress the ERK1/2 caused by LPS is mediated, the activation of JNK, p38 and the degraded of I κ B.
（7）Salix Babylonica L.P.E can be used for treating various pain and inflammation, its main active salicin can be shown The content for reducing proinflammatory factor (TNF-α, IL-1 β, IL-6) in RAW264.7 cells is write, increases anti-inflammatory factors (IL-10) Content.
（8）The traditional Chinese medicine extract composition with anti-skin irritatin effect mixed by above-mentioned Chinese medical extract, Energy multipath releives sensitive skin to extraneous stimulation, without steroids, antihistaminic etc., so as to avoid chemicals to skin The stimulation of skin and sensitization.
Brief description of the drawings
Fig. 1 is the stream of the preparation method of the traditional Chinese medicine extract composition with anti-skin irritatin effect of the embodiment of the present invention Cheng Tu.
Embodiment
Below in conjunction with the attached drawing in the embodiment of the present invention, the technical solution in the embodiment of the present invention is carried out clear, complete Site preparation describes, it is clear that described embodiment is part of the embodiment of the present invention, instead of all the embodiments.Based on this hair Embodiment in bright, the every other implementation that those of ordinary skill in the art are obtained without making creative work Example, belongs to the scope of protection of the invention.
Embodiment 1
A kind of traditional Chinese medicine extract composition with anti-skin irritatin effect of the present invention, weighs by medicinal material dry weight, includes radix glycyrrhizae 1 Part, 5 parts of kuh-seng, 10 parts of radix scutellariae, 15 parts of Angelica sinensis, 20 parts of Ligusticum wallichii, 20 parts of schizonepeta, 13 parts windproof, 16 parts of salicis,cortex.
As shown in Figure 1, a kind of preparation method of the traditional Chinese medicine extract composition with anti-skin irritatin effect, is used to prepare The above-mentioned traditional Chinese medicine extract composition with anti-skin irritatin effect, specifically includes following steps：
S10, prepare radix glycyrrhizae, kuh-seng, radix scutellariae, Angelica sinensis, Ligusticum wallichii, schizonepeta, windproof and salicis,cortex ethanol extract, specifically include with Lower step：
S101, measures 1 gram of radix glycyrrhizae, 5 grams of kuh-seng, 10 grams of radix scutellariae, 15 grams of Angelica sinensis, 20 grams of Ligusticum wallichii, 20 grams of schizonepeta, 13 grams windproof, white 16 grams of willow bark, note gross weight are numerical value A, in the present embodiment, A 100；
S102, in units of milliliter, the volume for remembering ethanol water is numerical value B, and measuring ethanol water makes value B be numerical value 5-20 times of A, in the present embodiment, it is 500 to take B；
S103, Chinese medicine soak extraction in 20% ethanol water, extraction time is 1 time, when immersion 2 is small every time, merges ethanol Extracting solution.
S20, concentrates 10 times after ethanol extract filtering, is purified with macroporous absorbent resin, water-soluble using 10% ethanol Liquid collects eluent as eluant, eluent after elution.
S30, after eluent concentrates 10 times, adds 1,3-BDO, the additive amount of 1,3-BDO is the volume of eluent 0.5 times, stir evenly, stand 24 it is small when, collect supernatant.
S40, is centrifuged supernatant, obtains centrifuged supernatant, being included in centrifuged supernatant has anti-skin thorn Swash the traditional Chinese medicine extract composition of effect.
Embodiment 2
A kind of traditional Chinese medicine extract composition with anti-skin irritatin effect of the present invention, weighs by medicinal material dry weight, includes radix glycyrrhizae 5 Part, 1 part of kuh-seng, 20 parts of radix scutellariae, 10 parts of Angelica sinensis, 15 parts of Ligusticum wallichii, 20 parts of schizonepeta, 16 parts windproof, 13 parts of salicis,cortex.
As shown in Figure 1, a kind of preparation method of the traditional Chinese medicine extract composition with anti-skin irritatin effect, is used to prepare The above-mentioned traditional Chinese medicine extract composition with anti-skin irritatin effect, specifically includes following steps：
S10, prepare radix glycyrrhizae, kuh-seng, radix scutellariae, Angelica sinensis, Ligusticum wallichii, schizonepeta, windproof and salicis,cortex ethanol extract, specifically include with Lower step：
S101, measures 5 grams of radix glycyrrhizae, 1 gram of kuh-seng, 20 grams of radix scutellariae, 10 grams of Angelica sinensis, 15 grams of Ligusticum wallichii, 20 grams of schizonepeta, 16 grams windproof, white 13 grams of willow bark, note gross weight are numerical value A, in the present embodiment, A 100；
S102, in units of milliliter, the volume for remembering ethanol water is numerical value B, and measuring 28% ethanol water makes value B For 5-20 times of numerical value A, in the present embodiment, it is 800 to take B；
S103, Chinese medicine soak extraction in 28% ethanol water, extraction time is 2 times, when immersion 6 is small every time, merges ethanol Extracting solution.
S20, concentrates 15 times after ethanol extract filtering, is purified with macroporous absorbent resin, using 25% ethanol water As eluant, eluent, eluent is collected after elution.
S30, after eluent concentrates 11.5 times, adds 1,3-BDO, the additive amount of 1,3-BDO is the body of eluent Long-pending 1.8 times, stir evenly, and when standing 24 is small, collect supernatant.
S40, is centrifuged supernatant, obtains centrifuged supernatant, being included in centrifuged supernatant has anti-skin thorn Swash the traditional Chinese medicine extract composition of effect.
Embodiment 3
A kind of traditional Chinese medicine extract composition with anti-skin irritatin effect of the present invention, weighs by medicinal material dry weight, includes radix glycyrrhizae 13 parts, 16 parts of kuh-seng, 1 part of radix scutellariae, 5 parts of Angelica sinensis, 10 parts of Ligusticum wallichii, 15 parts of schizonepeta, 20 parts windproof, 20 parts of salicis,cortex.
As shown in Figure 1, a kind of preparation method of the traditional Chinese medicine extract composition with anti-skin irritatin effect, is used to prepare The above-mentioned traditional Chinese medicine extract composition with anti-skin irritatin effect, specifically includes following steps：
S10, prepare radix glycyrrhizae, kuh-seng, radix scutellariae, Angelica sinensis, Ligusticum wallichii, schizonepeta, windproof and salicis,cortex ethanol extract, specifically include with Lower step：
S101, measures 13 grams of radix glycyrrhizae, 16 grams of kuh-seng, 1 gram of radix scutellariae, 5 grams of Angelica sinensis, 10 grams of Ligusticum wallichii, 15 grams of schizonepeta, 20 grams windproof, white 20 grams of willow bark, note gross weight are numerical value A, in the present embodiment, A 100；
S102, in units of milliliter, the volume for remembering ethanol water is numerical value B, and measuring ethanol water makes value B be numerical value 5-20 times of A, in the present embodiment, it is 1000 to take B；
S103, Chinese medicine soak extraction in 28% ethanol water, extraction time is 3 times, when immersion 12 is small every time, merges second Alcohol extract.
S20, concentrates 20 times after ethanol extract filtering, is purified with macroporous absorbent resin, using 40% ethanol water As eluant, eluent, eluent is collected after elution.
S30, after eluent concentrates 13 times, adds 1,3-BDO, the additive amount of 1,3-BDO is the volume of eluent 1.1 times, stir evenly, stand 24 it is small when, collect supernatant.
S40, is centrifuged supernatant, obtains centrifuged supernatant, being included in centrifuged supernatant has anti-skin thorn Swash the traditional Chinese medicine extract composition of effect.
Embodiment 4
A kind of traditional Chinese medicine extract composition with anti-skin irritatin effect of the present invention, weighs by medicinal material dry weight, includes radix glycyrrhizae 16 parts, 20 parts of kuh-seng, 5 parts of radix scutellariae, 1 part of Angelica sinensis, 13 parts of Ligusticum wallichii, 10 parts of schizonepeta, 15 parts windproof, 20 parts of salicis,cortex.
As shown in Figure 1, a kind of preparation method of the traditional Chinese medicine extract composition with anti-skin irritatin effect, is used to prepare The above-mentioned traditional Chinese medicine extract composition with anti-skin irritatin effect, specifically includes following steps：
S10, prepare radix glycyrrhizae, kuh-seng, radix scutellariae, Angelica sinensis, Ligusticum wallichii, schizonepeta, windproof and salicis,cortex ethanol extract, specifically include with Lower step：
S101, measures 16 grams of radix glycyrrhizae, 20 grams of kuh-seng, 5 grams of radix scutellariae, 1 gram of Angelica sinensis, 13 grams of Ligusticum wallichii, 10 grams of schizonepeta, 15 grams windproof, white 20 grams of willow bark, note gross weight are numerical value A, in the present embodiment, A 100；
S102, in units of milliliter, the volume for remembering ethanol water is numerical value B, and measuring ethanol water makes value B be numerical value 5-20 times of A, in the present embodiment, it is 1200 to take B；
S103, Chinese medicine soak extraction in 44% ethanol water, extraction time are 1 time, merge ethanol extract.
S20, concentrates 25 times after ethanol extract filtering, is purified with macroporous absorbent resin, using 55% ethanol water As eluant, eluent, eluent is collected after elution.
S30, after eluent concentrates 14.5 times, adds 1,3-BDO, the additive amount of 1,3-BDO is the body of eluent Long-pending 1.4 times, stir evenly, and when standing 24 is small, collect supernatant.
S40, is centrifuged supernatant, obtains centrifuged supernatant, being included in centrifuged supernatant has anti-skin thorn Swash the traditional Chinese medicine extract composition of effect.
Embodiment 5
A kind of traditional Chinese medicine extract composition with anti-skin irritatin effect of the present invention, weighs by medicinal material dry weight, includes radix glycyrrhizae 20 parts, 13 parts of kuh-seng, 16 parts of radix scutellariae, 20 parts of Angelica sinensis, 1 part of Ligusticum wallichii, 5 parts of schizonepeta, 10 parts windproof, 15 parts of salicis,cortex.
As shown in Figure 1, a kind of preparation method of the traditional Chinese medicine extract composition with anti-skin irritatin effect, is used to prepare The above-mentioned traditional Chinese medicine extract composition with anti-skin irritatin effect, specifically includes following steps：
S10, prepare radix glycyrrhizae, kuh-seng, radix scutellariae, Angelica sinensis, Ligusticum wallichii, schizonepeta, windproof and salicis,cortex ethanol extract, specifically include with Lower step：
S101, measures 20 grams of radix glycyrrhizae, 13 grams of kuh-seng, 16 grams of radix scutellariae, 20 grams of Angelica sinensis, 1 gram of Ligusticum wallichii, 5 grams of schizonepeta, 10 grams windproof, white 15 grams of willow bark, note gross weight are numerical value A, in the present embodiment, A 100；
S102, in units of milliliter, the volume for remembering ethanol water is numerical value B, and measuring ethanol water makes value B be numerical value 5-20 times of A, in the present embodiment, it is 1500 to take B；
S103, Chinese medicine soak extraction in 52% ethanol water, extraction time is 2 times, when immersion 20 is small every time, merges second Alcohol extract.
S20, concentrates 30 times after ethanol extract filtering, is purified with macroporous absorbent resin, using 70% ethanol water As eluant, eluent, eluent is collected after elution.
S30, after eluent concentrates 16 times, adds 1,3-BDO, the additive amount of 1,3-BDO is the volume of eluent 1.7 times, stir evenly, stand 24 it is small when, collect supernatant.
S40, is centrifuged supernatant, obtains centrifuged supernatant, being included in centrifuged supernatant has anti-skin thorn Swash the traditional Chinese medicine extract composition of effect.
Embodiment 6
A kind of traditional Chinese medicine extract composition with anti-skin irritatin effect of the present invention, weighs by medicinal material dry weight, includes radix glycyrrhizae 16 parts, 20 parts of kuh-seng, 13 parts of radix scutellariae, 15 parts of Angelica sinensis, 5 parts of Ligusticum wallichii, 1 part of schizonepeta, 20 parts windproof, 10 parts of salicis,cortex.
As shown in Figure 1, a kind of preparation method of the traditional Chinese medicine extract composition with anti-skin irritatin effect, is used to prepare The above-mentioned traditional Chinese medicine extract composition with anti-skin irritatin effect, specifically includes following steps：
S10, prepare radix glycyrrhizae, kuh-seng, radix scutellariae, Angelica sinensis, Ligusticum wallichii, schizonepeta, windproof and salicis,cortex ethanol extract, specifically include with Lower step：
S101, measures 16 grams of radix glycyrrhizae, 20 grams of kuh-seng, 13 grams of radix scutellariae, 15 grams of Angelica sinensis, 5 grams of Ligusticum wallichii, 1 gram of schizonepeta, 20 grams windproof, white 10 grams of willow bark, note gross weight are numerical value A, in the present embodiment, A 100；
S102, in units of milliliter, the volume for remembering ethanol water is numerical value B, and measuring ethanol water makes value B be numerical value 5-20 times of A, in the present embodiment, it is 1600 to take B；
S103, Chinese medicine soak extraction in 60% ethanol water, extraction time is 3 times, when immersion 30 is small every time, merges second Alcohol extract.
S20, concentrates 35 times after ethanol extract filtering, is purified with macroporous absorbent resin, using 85% ethanol water As eluant, eluent, eluent is collected after elution.
S30, after eluent concentrates 17.5 times, adds 1,3-BDO, the additive amount of 1,3-BDO is the body of eluent Long-pending 2 times, stir evenly, and when standing 24 is small, collect supernatant.
S40, is centrifuged supernatant, obtains centrifuged supernatant, being included in centrifuged supernatant has anti-skin thorn Swash the traditional Chinese medicine extract composition of effect.
Embodiment 7
A kind of traditional Chinese medicine extract composition with anti-skin irritatin effect of the present invention, weighs by medicinal material dry weight, includes radix glycyrrhizae 20 grams, 10 grams of kuh-seng, 15 grams of radix scutellariae, 16 grams of Angelica sinensis, 20 grams of Ligusticum wallichii, 13 grams of schizonepeta, 1 gram windproof, 5 grams of salicis,cortex.
As shown in Figure 1, a kind of preparation method of the traditional Chinese medicine extract composition with anti-skin irritatin effect, is used to prepare The above-mentioned traditional Chinese medicine extract composition with anti-skin irritatin effect, specifically includes following steps：
S10, prepare radix glycyrrhizae, kuh-seng, radix scutellariae, Angelica sinensis, Ligusticum wallichii, schizonepeta, windproof and salicis,cortex ethanol extract, specifically include with Lower step：
S101, measures 20 grams of radix glycyrrhizae, 10 grams of kuh-seng, 15 grams of radix scutellariae, 16 grams of Angelica sinensis, 20 grams of Ligusticum wallichii, 13 grams of schizonepeta, 1 gram windproof, white 5 grams of willow bark, note gross weight are numerical value A, in the present embodiment, A 100；
S102, in units of milliliter, the volume for remembering ethanol water is numerical value B, and measuring ethanol water makes value B be numerical value 5-20 times of A, in the present embodiment, it is 1800 to take B；
S103, Chinese medicine soak extraction in 70% ethanol water, extraction time is 2 times, when immersion 40 is small every time, merges second Alcohol extract.
S20, concentrates 40 times after ethanol extract filtering, is purified with macroporous absorbent resin, using 90% ethanol water As eluant, eluent, eluent is collected after elution.
S30, after eluent concentrates 19 times, adds 1,3-BDO, the additive amount of 1,3-BDO is the volume of eluent 2.5 times, stir evenly, stand 24 it is small when, collect supernatant.
S40, is centrifuged supernatant, obtains centrifuged supernatant, being included in centrifuged supernatant has anti-skin thorn Swash the traditional Chinese medicine extract composition of effect.
Embodiment 8
A kind of traditional Chinese medicine extract composition with anti-skin irritatin effect of the present invention, weighs by medicinal material dry weight, includes radix glycyrrhizae 10 grams, 15 grams of kuh-seng, 20 grams of radix scutellariae, 13 grams of Angelica sinensis, 16 grams of Ligusticum wallichii, 20 grams of schizonepeta, 5 grams windproof, 1 gram of salicis,cortex.
As shown in Figure 1, a kind of preparation method of the traditional Chinese medicine extract composition with anti-skin irritatin effect, is used to prepare The above-mentioned traditional Chinese medicine extract composition with anti-skin irritatin effect, specifically includes following steps：
S10, prepare radix glycyrrhizae, kuh-seng, radix scutellariae, Angelica sinensis, Ligusticum wallichii, schizonepeta, windproof and salicis,cortex ethanol extract, specifically include with Lower step：
S101, measures 10 grams of radix glycyrrhizae, 15 grams of kuh-seng, 20 grams of radix scutellariae, 13 grams of Angelica sinensis, 16 grams of Ligusticum wallichii, 20 grams of schizonepeta, 5 grams windproof, white 1 gram of willow bark, note gross weight are numerical value A, in the present embodiment, A 100；
S102, in units of milliliter, the volume for remembering ethanol water is numerical value B, and measuring ethanol water makes value B be numerical value 5-20 times of A, in the present embodiment, it is 2000 to take B；
S103, Chinese medicine soak extraction in 80% ethanol water, extraction time is 3 times, when immersion 48 is small every time, merges second Alcohol extract.
S20, concentrates 50 times after ethanol extract filtering, is purified with macroporous absorbent resin, using 95% ethanol water As eluant, eluent, eluent is collected after elution.
S30, after eluent concentrates 20 times, adds 1,3-BDO, the additive amount of 1,3-BDO is the volume of eluent 3 times, stir evenly, stand 24 it is small when, collect supernatant.
S40, is centrifuged supernatant, obtains centrifuged supernatant, being included in centrifuged supernatant has anti-skin thorn Swash the traditional Chinese medicine extract composition of effect.
It should be appreciated that exemplary embodiment as described herein is illustrative and be not restrictive.Although retouched with reference to attached drawing One or more embodiments of the invention is stated, it should be understood by one skilled in the art that without departing from appended right It is required that in the case of the spirit and scope of the present invention limited, the change of various forms and details can be made.

Claims (9)

  1. A kind of 1. traditional Chinese medicine extract composition with anti-skin irritatin effect, it is characterised in that comprising radix glycyrrhizae, kuh-seng, radix scutellariae, Angelica sinensis, Ligusticum wallichii, schizonepeta, windproof and salicis,cortex extract.
  2. 2. the traditional Chinese medicine extract composition according to claim 1 with anti-skin irritatin effect, it is characterised in that by medicine The percentage by weight meter of material dry weight, includes radix glycyrrhizae 1-20%, kuh-seng 1-20%, radix scutellariae 1-20%, Angelica sinensis 1-20%, Ligusticum wallichii 1-20%, chaste tree Mustard 1-20%, windproof 1-20%, salicis,cortex 1-20%.
  3. 3. a kind of preparation method of the traditional Chinese medicine extract composition with anti-skin irritatin effect, it is characterised in that be used to prepare The traditional Chinese medicine extract composition with anti-skin irritatin effect described in claim 1 or 2, specifically includes following steps：
    S10, prepares radix glycyrrhizae, kuh-seng, radix scutellariae, Angelica sinensis, Ligusticum wallichii, schizonepeta, windproof and salicis,cortex ethanol extract；
    S20, concentrates after ethanol extract filtering, is purified with macroporous absorbent resin, eluent is collected after elution；
    S30, concentrates the eluent, adds 1,3-BDO, stirs evenly, and when standing 24 is small, collects supernatant；
    S40, is centrifuged the supernatant, obtains centrifuged supernatant, has in the centrifuged supernatant comprising described The traditional Chinese medicine extract composition of anti-skin irritatin effect.
  4. 4. the preparation method of the traditional Chinese medicine extract composition of anti-skin irritatin effect according to claim 3, its feature exist In S10 specifically includes following steps：
    S101, in grams, measures 1-20 parts of radix glycyrrhizae, 1-20 parts of kuh-seng, 1-20 parts of radix scutellariae, 1-20 parts of Angelica sinensis, Ligusticum wallichii 1-20 Part, 1-20 parts of schizonepeta, 1-20 parts windproof, 1-20 parts of salicis,cortex, note gross weight are numerical value A；
    S102, in units of milliliter, the volume for remembering ethanol water is numerical value B, and measuring the ethanol water of 20%-80% makes total Value B is 5-20 times of numerical value A；
    S103, Chinese medicine soak extraction in the ethanol water of the 20%-80%, extraction time are 1-3 times, merge ethanol extraction Liquid.
  5. 5. the preparation method of the traditional Chinese medicine extract composition of anti-skin irritatin effect according to claim 4, its feature exist In in S103, when the time of soak extraction is 2-48 small each time.
  6. 6. the preparation method of the traditional Chinese medicine extract composition of anti-skin irritatin effect according to claim 3, its feature exist In in S20, the ethanol extract concentrates 10-50 times.
  7. 7. the preparation method of the traditional Chinese medicine extract composition of anti-skin irritatin effect according to claim 3, its feature exist In, in S20, ethanol water that elution step is 10-95% with eluant, eluent.
  8. 8. the preparation method of the traditional Chinese medicine extract composition of anti-skin irritatin effect according to claim 3, its feature exist In in S30, the eluent concentrates 10-20 times.
  9. 9. the preparation method of the traditional Chinese medicine extract composition of anti-skin irritatin effect according to claim 8, its feature exist In in S30, the additive amount of 1,3-BDO is 0.5-3 times of the volume of eluent.
CN201610937313.9A 2016-11-01 2016-11-01 A kind of traditional Chinese medicine extract composition with anti-skin irritatin effect and preparation method thereof Pending CN108014202A (en)

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CN109568220A (en) * 2019-02-14 2019-04-05 重庆市洲仨科技发展有限公司 A kind of compound polyphenol antiallergic essence and antiallergic essence lotion
CN109846777A (en) * 2019-02-01 2019-06-07 上海珈凯生物科技有限公司 It is a kind of to have effects that alleviate composition of plant extracts of baby child's eczema and preparation method thereof, application
CN109864930A (en) * 2018-12-29 2019-06-11 上海珈凯生物科技有限公司 A kind of composition of plant extracts with inflammation-resisting itch-stopping and preparation method thereof, application
CN110638861A (en) * 2019-10-30 2020-01-03 上海珈凯生物科技有限公司 Application of composition in skin barrier gene repair
CN112870131A (en) * 2021-03-31 2021-06-01 深圳市萱嘉生物科技有限公司 Traditional Chinese medicine extract composition with acne removing effect and preparation method thereof

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109864930A (en) * 2018-12-29 2019-06-11 上海珈凯生物科技有限公司 A kind of composition of plant extracts with inflammation-resisting itch-stopping and preparation method thereof, application
CN109846777A (en) * 2019-02-01 2019-06-07 上海珈凯生物科技有限公司 It is a kind of to have effects that alleviate composition of plant extracts of baby child's eczema and preparation method thereof, application
CN109568220A (en) * 2019-02-14 2019-04-05 重庆市洲仨科技发展有限公司 A kind of compound polyphenol antiallergic essence and antiallergic essence lotion
CN110638861A (en) * 2019-10-30 2020-01-03 上海珈凯生物科技有限公司 Application of composition in skin barrier gene repair
CN112870131A (en) * 2021-03-31 2021-06-01 深圳市萱嘉生物科技有限公司 Traditional Chinese medicine extract composition with acne removing effect and preparation method thereof

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Application publication date: 20180511