CN107988120A - Microbial bacterial agent and its preparation method and application - Google Patents
Microbial bacterial agent and its preparation method and application Download PDFInfo
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- CN107988120A CN107988120A CN201711482604.4A CN201711482604A CN107988120A CN 107988120 A CN107988120 A CN 107988120A CN 201711482604 A CN201711482604 A CN 201711482604A CN 107988120 A CN107988120 A CN 107988120A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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Abstract
The present invention relates to a kind of microbial bacterial agent and its preparation method and application, belong to the integrated control field of plant disease.Including functional microorganism, nutritional agents and protective agent；The microorganism includes Streptomyces jingyangensis, Strepiomyces lavendulae, streptomyces aureus and Trichoderma viride.The functional microorganism screening of microbial bacterial agent is used and played a role in a manner of bacterium is antibacterial, kill pathogenic bacteria, will not cause environmental pollution, reduce the use of medicine in nature；Microbial bacterial agent is safe and harmless, adverse effect will not be produced to crop and human body, it can be used for plant sprinkling, seed immersion, seedling spraying, irrigation water sterilization, farm implements disinfection and soil treatment etc., the route of transmission of pathogenic bacteria, the generation of controlling disease blocked from number of ways；Microbial bacterial agent application method is flexible, safe, simple.
Description
Technical field
The present invention relates to a kind of microbial bacterial agent and its preparation method and application, the integrated control for belonging to plant disease is led
Domain.
Background technology
Canker of tomato is one of most serious disease in tomato production, since the U.S. reports, has been spread rapidly from first
And the multiple tomato main producing regions in the world are traveled to, it is increasingly becoming a kind of worldwide disease.The disease is dispersed throughout North America, Europe at present
Continent, Asia, Africa, Oceania, cover more than 60 countries such as the U.S., Britain, Canada, Germany, Japan.China was in 1985
Canker of tomato is found in Pinggu County, beijing, expanded to multiple tomato producing regions first.China northeast is confirmed first within 1993
There is the generation of canker of tomato in area, and 2002 in Anhui Guo Ning cities Fang Tang townshiies tomato incidence more than 80%, 2007~2009
Year loses in Guizhou Province Xiuwen County as caused by canker of tomato reaches 40%~50%.Recently as the anomalous variation of weather
And the expansion of tomato planting scale, canker of tomato have the trend aggravated year by year, at present in China Heilungkiang, Jilin, Liaoning, interior
There is different degrees of generation on the ground such as Mongolia, Xinjiang, Hebei, Henan, Shandong, Shanghai, Hainan, Chongqing, Hubei, Guizhou, to work as
The tomato planting on ground exerts a certain influence, and is the only one disease that China quarantines tomato disease.
Bacterial canker of tomato also infects capsicum, black nightshade, three-Flowered nightshade and other tomato genus plants, also may be used in addition to tomato is infected
Colonize in Nicotiana glutinosa and other nightshades.Pathogenic bacteria can be overwintering on tomato plant, seed, invalid body, soil and weeds,
It can survive 20 years, can be propagated by number of ways such as seed, seedling, rainwater, insect, farming operations on dry seed, be endangered
It is heavier, involve that scope is wider, therefore the pests occurrence rule and Prevention Technique of Tomato Caused by Clavibacter michiganensis subsp. michiganensis are grasped, for controlling the disease
Generation it is significant.
Canker of tomato mainly aims at prevention at present, and carrying out control of the prevention work to disease at the initial stage of a disease can play
Preferable effect, the method for use mainly have reinforcement quarantine, seed treatment, soil treatment, shift of crops, chemical control etc. to arrange
Apply, but these methods are not thorough there are processing, pollute environment, influence many drawbacks such as plant growth, and also it is palliative,
Pathogenic bacteria are not killed from root, can only temporarily slow down the generation of disease, once prevention and control measure fails, disease can be sent out once again
It is raw.
The content of the invention
In view of the foregoing defects the prior art has, the present invention proposes a kind of microbial bacterial agent and preparation method thereof and should
With.
The present invention is realized using following technical solution：
A kind of microbial bacterial agent, including functional microorganism, nutritional agents and protective agent；The microorganism includes Streptomyces jingyangensis
Streptomyces chingyangensis, Strepiomyces lavendulae Streptomyces fradiae, streptomyces aureus
Streptomyces aureus and Trichoderma viride Trichoderma viride.
The percentage by weight of the microbial bacterial agent is functional microorganism 50%~80%, nutritional agents 10%~30%, protects
Protect agent 10%~30%.
The nutritional agents includes glucose, whey powder and magnesium sulfate, its mass ratio is 40~70:30~60:2~5.
The protective agent includes trehalose, ascorbic acid and copper sulphate, its mass ratio is 30~60:30~60:5~
10。
A kind of preparation method of microbial bacterial agent as described above, functional microorganism, nutritional agents and protective agent is mixed equal
It is even, vacuum packaging.
A kind of application of microbial bacterial agent as described above in canker of tomato is prevented.
During for plant sprinkling, seed or seedling dip-coating, by 1000~2000 times of uses of microbial bacteria dilution agent.
During for farm implements disinfection or soil treatment, by 500~1500 times of uses of microbial bacteria dilution agent.
When being applied for adding in irrigation water punching, microbial bacterial agents are added by 150~300g/ mu.
The beneficial effects of the invention are as follows：
The functional microorganism screening of microbial bacterial agent is used and played a role in a manner of bacterium is antibacterial, kill and cause in nature
Germ, will not cause environmental pollution, reduce the use of medicine；Microbial bacterial agent is safe and harmless, and crop and human body will not be produced
Adverse effect, can be used for plant sprinkling, seed immersion, seedling spraying, irrigation water sterilization, farm implements disinfection and soil treatment etc.,
The route of transmission of pathogenic bacteria, the generation of controlling disease are blocked from number of ways；Microbial bacterial agent application method flexibly, safety, letter
It is single.
Embodiment
In order to which the object of the invention, technical solution is more clearly understood, below by embodiment, the present invention is made further
Describe in detail.
Embodiment one：
The screening of functional microorganism
In canker of tomato Infected regions, the region collection pedotheque that degree of disease is lighter, and disease plant are selected
Do not occur the leaf sample of disease, carry out the separation, culture and preservation of microorganism, by preliminary screening, pedotheque separates
To 7 plants of strains, numbering A01-A07, the isolated 4 plants of strains of leaf sample, numbering A08-A11.At the same time from laboratory strains storehouse
In select the bacillus subtilis for control of plant disease, bacillus laterosporus, bacillusmusilaginosiengineering, Trichoderma harzianum, wheel
9 plants of branch bacterium, Streptomyces jingyangensis, Paecilomyces lilacinus, yellow trichoderma, Hyperparasite of Powdery Mildew strains.11 plants of primary dcreening operation strains and 9 plants are tested
Room strain carries out bacteriostatic test, observes inhibitory action of each strain to canker of tomato pathogenic bacteria.Concrete outcome is as follows：
Note：" +++ " represents significant effect, and " ++ " represents positive effect, and "+" represents that effect is general, and "-" represents not imitate
Fruit.
Can be seen that from antibacterial result in primary dcreening operation strain has 5 plants of bacterium to have obvious prevention effect, by colonial morphology point
Analysis and strain Molecular Identification, it is Strepiomyces lavendulae to determine A02 and A10, and A07 is streptomyces aureus, A04 and A09 wooden for green
It is mould, i.e., actually screen 3 plants of strains；It is bacillus laterosporus that laboratory strains, which have the strain of prevention effect, gel-shaped gemma bar
Bacterium, Streptomyces jingyangensis and Trichoderma viride.Found by application experiment, bacillus laterosporus and bacillusmusilaginosiengineering were using
Growth and breeding ability in journey is weaker, and the adaptability that different strain is shown in different environments is different, therefore chooses
4 plants of Strepiomyces lavendulae, streptomyces aureus, Trichoderma viride, Streptomyces jingyangensis strains, the system by the way of multiple-microorganism compounding
Standby microbial bacterial agent, according to the corresponding strain combination of different application environmental selection.
Embodiment two：
Prepare microbial bacterial agent
A:By Streptomyces jingyangensis, Strepiomyces lavendulae, streptomyces aureus, Trichoderma viride according to 1:1:1:1 ratio mixing
Microbial germ powder is made
B：By glucose, whey powder, magnesium sulfate according to 60:35:5 ratio mixing, is prepared into nutritional agents.
C：By trehalose, ascorbic acid, copper sulphate according to 50:40:10 ratio mixing, prepares protective agent.
D：Microbial germ powder, nutritional agents, protective agent are uniformly mixed, is vacuum-packed, microbial bacterial agent is prepared, measures
Viable bacteria amount is 2,400,000,000/g in microbial inoculum.
Embodiment three：
The fungistatic effect and dosage of microbial bacterial agent are investigated by testing
Tomato seedling is planted using potted plant experiment, the consistent seedling of growing way is picked out after 5 days, by the way of artificial infection
Pathogenic bacteria are inoculated with, it is spare to be put into the different zones kept apart after disease symptom to appear.Microbial bacteria prepared by embodiment one
Agent dilutes 500 times, 1000 times, 1500 times, 2000 times, 2500 times, 3000 times of bacterium solutions for being prepared into various concentrations respectively, according to just
Often spray speed sprays the seedling to have fallen ill.Then conventionally it is managed, sprinkling in every 7 days once, is observed
Plant incidence.
It is found through experiments that, dilute the group of 2500 times and 3000 times bacterium solutions, the sprawling of disease symptom substantially slows down, but still
Slowly diffusion, illustrates that dilution is excessive, reduces the preventive and therapeutic effect of microbial inoculum；500 times, 1000 times, 1500 times and 2000 times of dilution
Microbial inoculum can effectively play a role, and site of pathological change is not further continued for spreading, also occur without new site of pathological change, illustrate dilution
Bacterium solution within 2000 times can be very good to play a role, can be by the way of 1000 times and 1500 times of dilution to ensure effect
Use.
Example IV：
Verify that microbial bacterial agent prevents the actual effect of canker of tomato by application experiment
The recent region that canker of tomato occurred is chosen from tomato species growing area to be tested, experiment is divided into three groups, respectively
It is without any processing for control group, medicament group and microorganism group, control group；Medicament group uses 20% network ammonia copper water 500 times of liquid of agent,
Sprayed every 7 days 1 time；Microorganism group uses microbial bacterial agent prepared by embodiment one, dilutes 1000 times of uses, was sprayed every 7 days
Apply 1 time.Quarantine measures are taken between each test area, prevent from influencing each other.100 plants of tomato seedlings of each group plantation, experiment periods
For 30 days, routinely plantation management method carried out other conditions.Experimental result is as follows：
| Group | Control group | Medicament group | Microorganism group |
| Morbidity strain number | 32 | 6 | 5 |
| Incidence | 32% | 6% | 5% |
The result shows that medicament group and microorganism group are all obvious to the prevention effect of canker of tomato, can effectively reach
To preventive and therapeutic effect.But medicament can cause crop residual and soil pollution, therefore Comprehensive Correlation, microbial bacterial agent are better than chemical drugs
Agent.
Embodiment five：
The persistence of prevention effect is verified by application experiment
Experiment is divided into three groups, is respectively medicament group, microorganism and microorganism+soil group.Medicament group uses 20% network ammonia copper
500 times of liquid of aqua, sprayed 1 time every 7 days；Microorganism group uses microbial bacterial agent prepared by embodiment one, and 1000 times of dilution makes
With being sprayed every 7 days 1 time；Microorganism+soil group begins with microorganism in addition to normally sprinkling microbial bacterial agent in experiment
Microbial inoculum processing soil is once.Three groups respectively spray 4 times after stop spraying, the 30th day will morbidity tomato plants count after remove, continue
Observation 20 days, investigates plant incidence.Experimental result is as follows：
| Group | Medicament group | Microorganism group | Microorganism+soil group |
| 30th day plant sum | 92 | 91 | 97 |
| Morbidity strain number | 23 | 14 | 0 |
| Incidence | 25% | 15% | 0% |
From experimental result as can be seen that incidence is substantially increased after medicament group disables medicine, up to 25%, illustrate medicament energy
Enough contain pathogenic bacteria morbidity, but cannot fundamentally solve the problems, such as disease；Microorganism group incidence is less than medicament group, but is also up to
15%, aggravation trend is obvious.The incidence of medicament group and microorganism group is significantly raised, and reason is that both main functions are to kill
Go out the pathogenic bacteria of aerial part, and the effect to pathogenic bacteria in soil is extremely limited, therefore the 3rd group has specially been investigated microorganism
The influence of soil treatment.From data as can be seen that using microbial bacterial agent to planting area under ground portion and aerial part at the same time
Extremely obvious to the prevention effect of canker of tomato after processing, in addition to 3 plants of disease plants occurs in initial stage, the later stage does not occur again
Disease plant.
The foregoing is merely presently preferred embodiments of the present invention, oneself, is not limitation with the present invention, all in essence of the invention
Impartial modifications, equivalent substitutions and improvements made within refreshing and principle etc., should be included in the patent covering scope of the present invention.
Claims (9)
1. a kind of microbial bacterial agent, it is characterised in that including functional microorganism, nutritional agents and protective agent；The microorganism includes
Streptomyces jingyangensis Streptomyces chingyangensis, Strepiomyces lavendulae Streptomyces fradiae, golden chain
Mould Streptomyces aureus and Trichoderma viride Trichoderma viride.
2. microbial bacterial agent according to claim 1, it is characterised in that the percentage by weight of the microbial bacterial agent is work(
Can microorganism 50%~80%, nutritional agents 10%~30%, protective agent 10%~30%.
3. microbial bacterial agent according to claim 1, it is characterised in that the nutritional agents include glucose, whey powder and
Magnesium sulfate, its mass ratio are 40~70:30~60:2~5.
4. microbial bacterial agent according to claim 1, it is characterised in that the protective agent includes trehalose, ascorbic acid
And copper sulphate, its mass ratio are 30~60:30~60:5~10.
5. a kind of preparation method of the microbial bacterial agent as described in claim 1-4, it is characterised in that by functional microorganism, nutrition
Agent and protective agent are uniformly mixed, vacuum packaging.
6. a kind of microbial bacterial agent as described in claim 1-4 is applied in canker of tomato is prevented.
7. application according to claim 6, it is characterised in that during for plant sprinkling, seed or seedling dip-coating, by micro- life
Thing microbial inoculum dilutes 1000~2000 times of uses.
8. application according to claim 6, it is characterised in that during for farm implements disinfection or soil treatment, by microbial bacteria
500~1500 times of uses of dilution agent.
9. application according to claim 6, it is characterised in that when being applied for adding in irrigation water punching, by 150~300g/
Mu addition microbial bacterial agent.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1490280A (en) * | 2003-09-08 | 2004-04-21 | 中国农业科学院土壤肥料研究所 | Microorganic fertilizer against various bacteria and its preparation and use |
| CN101481669A (en) * | 2009-02-10 | 2009-07-15 | 西北农林科技大学 | Preparation and use of lilac grey streptomycete and active product thereof |
| CN103602607A (en) * | 2013-07-31 | 2014-02-26 | 江西农业大学 | One strain of streptomyceslavendulae X33 and applications thereof |
| CN105732136A (en) * | 2016-02-22 | 2016-07-06 | 湖南泰谷生物科技股份有限公司 | Special bio-organic fertilizer for pepper and preparation method thereof |
-
2017
- 2017-12-29 CN CN201711482604.4A patent/CN107988120B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1490280A (en) * | 2003-09-08 | 2004-04-21 | 中国农业科学院土壤肥料研究所 | Microorganic fertilizer against various bacteria and its preparation and use |
| CN101481669A (en) * | 2009-02-10 | 2009-07-15 | 西北农林科技大学 | Preparation and use of lilac grey streptomycete and active product thereof |
| CN103602607A (en) * | 2013-07-31 | 2014-02-26 | 江西农业大学 | One strain of streptomyceslavendulae X33 and applications thereof |
| CN105732136A (en) * | 2016-02-22 | 2016-07-06 | 湖南泰谷生物科技股份有限公司 | Special bio-organic fertilizer for pepper and preparation method thereof |
Non-Patent Citations (4)
| Title |
|---|
| G. FIUME ET AL.: "BIOLOGICAL CONTROL OF CORKY ROOT IN TOMATO", 《COMM. APPL. BIOL. SCI》 * |
| 张维宏 等: "番茄溃疡病菌拮抗链霉菌菌株23的鉴定", 《植物病理学报》 * |
| 张维宏 等: "番茄溃疡病菌颉颃链霉菌的筛选", 《第三届全国绿色环保农药新技术、新产品交流会暨第二届全国生物农药研讨会》 * |
| 徐孝华: "《普通微生物学》", 31 May 1992 * |
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