CN107980496A - A kind of implantation methods of dendrobium candidum - Google Patents

A kind of implantation methods of dendrobium candidum Download PDF

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Publication number
CN107980496A
CN107980496A CN201711257538.0A CN201711257538A CN107980496A CN 107980496 A CN107980496 A CN 107980496A CN 201711257538 A CN201711257538 A CN 201711257538A CN 107980496 A CN107980496 A CN 107980496A
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parts
extraction
seedbed
tissue
ultrasonic
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宋剑春
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Jiangxi Song Ling Hu Biotechnology Co Ltd
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Jiangxi Song Ling Hu Biotechnology Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity

Abstract

The invention discloses a kind of implantation methods of dendrobium candidum, step are as follows:Prepare cultivation matrix;In greenhouse, seedbed is built using hollow brick, by cultivation matrix spreading in seedbed;Tissue-cultured seedling is taken, the culture medium of tissue-cultured seedling root carrying is removed using clear water, after cleaning, the root of tissue-cultured seedling is put into pretreatment fluid, is soaked, is taken out, obtains pretreatment tissue-cultured seedling;It will pre-process on tissue culture transplantation of seedlings to seedbed, water into seedbed, and ensure the humidity of cultivation matrix in seedbed, and ensure the air humidity in greenhouse;After transplanting 20 days, an amino acid chelate fertilizer was sprayed every 7 days, and carried out disinfection once using carbendazim thimerosal every 15 days;After plantation 23 years, you can harvesting.The survival rate that the implantation methods of dendrobium candidum of the present invention can improve tissue-cultured seedling is high, makes dendrobium candidum growing way more preferable, improves the yield of dendrobium candidum, to meet ever-increasing market demand, has a vast market prospect.

Description

A kind of implantation methods of dendrobium candidum
Technical field
The present invention relates to dendrobium candidum Cultivating techniques field, is specifically a kind of implantation methods of dendrobium candidum.
Background technology
Iron sheet stone solution is orchid family stone solution platymiscium, is China's tradition rare traditional Chinese medicine, and the Yi Bei worlds are classified as a kind of protection and plant Thing.Because its yield is extremely rare, growing environment is special and nourishing effects are distinguished, it is known as " a thousand pieces of gold grass ", " soft gold " and " plant is yellow The great fame of gold ".Its nature and flavor is sweet, flat, returns stomach, lung, kidney channel, energy tonifying five zang organs blood, essence, Tianjin, flat stomach gas, long term usage thickness stomach, coordinate cloudy Sun.It is the good medicine for treating the disease such as the deficiency of Yin, mesh is dark, stomach is weak, hoarseness, the plantation of iron sheet stone solution is especially to improving human immunity Power, anti-aging, lower hyperlipidemia, hypertension, hyperglycemia (hyperlipidemia, hyperglycaemia, hypertension), suppression tumour etc. play the role of auxiliary, have immune Adjust, slow down aging and other effects.It is not only the required raw material of familiar " MAILUONING ZHUSHEYE ", or tens of kinds of Chinese patent drugs And the necessary raw material of health products.However, dendrobium candidum requires growing environment harsh, existing dendrobium candidum implantation methods yield It is relatively low, it is difficult to meet ever-increasing market demand.
The content of the invention
It is proposed in the above background technology to solve it is an object of the invention to provide a kind of implantation methods of dendrobium candidum Problem.
To achieve the above object, the present invention provides following technical solution:
A kind of implantation methods of dendrobium candidum, step are as follows:
1)Prepare cultivation matrix, the cultivation matrix is made of the raw material below according to parts by weight:75-80 parts of peanut shell, pear tree 200-210 parts of skin, 40-45 parts of maize cob meal, 52-58 parts of rice husk, 25-30 parts of cow dung, 8-12 parts of humic acid, 15-20 parts of shell, 6-10 parts of urea, 2-5 parts of ammonium phosphate, 1-3 parts of potassium sulfate, 1-2 parts of ammonium molybdate, play both sides of the street 5-9 parts;
2)In greenhouse, seedbed is built using hollow brick, seedbed is 50-60cm apart from ground level, and seedbed width is 1.8- 2.2m, by cultivation matrix spreading in seedbed, the spreading thickness of cultivation matrix is 15-20cm;
3)Tissue-cultured seedling is taken, the culture medium of tissue-cultured seedling root carrying is removed using clear water, after cleaning, the root of tissue-cultured seedling is put Enter in pretreatment fluid, soak 20-24h, take out, obtain pretreatment tissue-cultured seedling, the pretreatment fluid is few using thick wood-fern rhizome and shell Sugar is made;
4)It will pre-process on tissue culture transplantation of seedlings to seedbed, and be planted at 25-30 DEG C, watered into seedbed, ensure in seedbed The humidity of cultivation matrix is 35-40%, and humidifier is placed in greenhouse, ensures that the air humidity in greenhouse is 60-70%;
5)After transplanting 20 days, an amino acid chelate fertilizer was sprayed every 7 days, and carried out using carbendazim thimerosal every 15 days Disinfection is once;
6)After 2-3 is planted, you can harvesting.
As the further scheme of the present invention:The preparation method of the cultivation matrix is as follows:
a)Take and play both sides of the street, clean, drying, adds the ethanol water of 15-20 times of weight, soak 48-60h, then at room temperature Historrhexis's extraction is carried out, the time of historrhexis's extraction is 6-8min, filtering, obtains historrhexis's extraction filtrate and tissue is broken Historrhexis's extraction filter residue, is added the ethanol water of 8-10 times of weight, soaks 2-3h, be sent at ultrasonic wave by broken extraction filter residue Manage in device, ultrasonic extraction 50-60min, filtering, obtain ultrasonic extraction liquid and ultrasonic extraction residue, the ultrasonic extraction to play both sides of the street are residual Slag is spare, and historrhexis then is extracted filtrate and ultrasonic extraction liquid merges, negative pressure evaporation concentration is the 10% of cumulative volume, is stood 24h, filters out sediment, up to Fructus Lipuidambaris extract;
b)Peanut shell, pear tree skin, maize cob meal and rice husk are weighed, after merging, is put into mixer, adds gross weight 35-40% Water, after being uniformly mixed, obtain mixture A;
c)Cow dung and shell are weighed, cow dung is dried 3-5 days, after shell is crushed, is added in the cow dung to after drying, stirring is mixed After closing uniformly, mixture B is obtained;
d)Mixture A and mixture B is merged, after being uniformly mixed, piles height 1m, the material heap of width 2m, and expecting heap Mulch in upper cover, carries out spontaneous fermentation;
e)After expecting that heap temperature reaches 55 DEG C, mulch is raised, admixes humic acid and the ultrasonic extraction residue to play both sides of the street, after mixing thoroughly, Mulch is covered, after continuing fermentation 3-4 days, mulch is raised, obtains fermentation material;
f)Urea, ammonium phosphate, potassium sulfate and ammonium molybdate are weighed, by Fructus Lipuidambaris extract, urea, ammonium phosphate, potassium sulfate and molybdic acid Ammonium is added into fermentation material, after being uniformly mixed, is sent into high voltage sterilizing device, is gone out using vapor under 200 kPas Bacterium handles 2-3h, after natural cooling, up to cultivation matrix.
As further scheme of the invention:Step a)In, the bath temperature of the ultrasonic extraction is 50-55 DEG C, is surpassed The frequency of sound extraction is 30-40kHz.
As further scheme of the invention:Concentration of alcohol in the ethanol water is 50%.
As further scheme of the invention:The preparation method of the pretreatment fluid is as follows:Thick wood-fern rhizome is taken, is cleaned, Drying, adds the ethanol water of 8-10 times of weight, soaks 2-3h, heating and refluxing extraction 50-60min, obtains reflux extracting liquid With refluxing extraction residue, the ethanol water of 5-6 times of weight is added into refluxing extraction residue, soaks 1-2h, ultrasonic wave extraction 40-50min, obtains ultrasonic extract and ultrasonic wave extraction residue, and reflux extracting liquid and ultrasonic extract are merged, decompression It is concentrated by evaporation as the 10% of original volume, stands 24h, filter out sediment, then adds the chitosan oligosaccharide of 50g/L, be uniformly mixed Afterwards, you can obtain pretreatment fluid.
As further scheme of the invention:The bath temperature of the ultrasonic extraction is 45-50 DEG C, the frequency of ultrasonic extraction Rate is 50-60kHz.
As further scheme of the invention:Concentration of alcohol in the ethanol water is 60%.
Compared with prior art, the beneficial effects of the invention are as follows:
The implantation methods of dendrobium candidum of the present invention, it is possible to increase the survival rate of tissue-cultured seedling is high, makes dendrobium candidum growing way more preferable, improves The yield of dendrobium candidum, to meet ever-increasing market demand, has a vast market prospect.
Embodiment
Technical scheme is described in more detail with reference to embodiment.
Embodiment 1
A kind of implantation methods of dendrobium candidum, step are as follows:
1)Prepare cultivation matrix, the cultivation matrix is made of the raw material below according to parts by weight:75 parts of peanut shell, pear tree skin 200 parts, 40 parts of maize cob meal, 52 parts of rice husk, 25 parts of cow dung, 8 parts of humic acid, 15 parts of shell, 6 parts of urea, 2 parts of ammonium phosphate, sulphur Sour 1 part of potassium, 1 part of ammonium molybdate, play both sides of the street 5 parts;
The preparation method of the cultivation matrix is as follows:
a)Take and play both sides of the street, clean, drying, adds the ethanol water of 15 times of weight, soak 48h, then carry out group at room temperature Broken extraction is knitted, the time of historrhexis's extraction is 6min, filtering, obtains historrhexis's extraction filtrate and historrhexis's extraction filter Historrhexis's extraction filter residue, is added the ethanol water of 8 times of weight, soaks 2h, be sent into processor for ultrasonic wave, ultrasound is carried by slag 50min is taken, is filtered, obtains ultrasonic extraction liquid and ultrasonic extraction residue, the ultrasonic extraction residue to play both sides of the street is spare, then by tissue Broken extraction filtrate and ultrasonic extraction liquid merge, and negative pressure evaporation concentration is the 10% of cumulative volume, stands 24h, filters out sediment, i.e., Fructus Lipuidambaris extract, wherein, the bath temperature of the ultrasonic extraction is 50 DEG C, and the frequency of ultrasonic extraction is 30kHz, the second Concentration of alcohol in alcohol solution is 50%;
b)Peanut shell, pear tree skin, maize cob meal and rice husk are weighed, after merging, is put into mixer, adds gross weight 35% Water, after being uniformly mixed, obtains mixture A;
c)Cow dung and shell are weighed, cow dung is dried 3 days, after shell is crushed, adds in the cow dung to after drying, is stirred After uniformly, mixture B is obtained;
d)Mixture A and mixture B is merged, after being uniformly mixed, piles height 1m, the material heap of width 2m, and expecting heap Mulch in upper cover, carries out spontaneous fermentation;
e)After expecting that heap temperature reaches 55 DEG C, mulch is raised, admixes humic acid and the ultrasonic extraction residue to play both sides of the street, after mixing thoroughly, Mulch is covered, after continuing fermentation 3 days, mulch is raised, obtains fermentation material;
f)Urea, ammonium phosphate, potassium sulfate and ammonium molybdate are weighed, by Fructus Lipuidambaris extract, urea, ammonium phosphate, potassium sulfate and molybdic acid Ammonium is added into fermentation material, after being uniformly mixed, is sent into high voltage sterilizing device, is gone out using vapor under 200 kPas Bacterium handles 2h, after natural cooling, up to cultivation matrix;
2)In greenhouse, seedbed is built using hollow brick, seedbed is 50cm apart from ground level, and seedbed width is 1.8m, will be planted Matrix spreading is trained in seedbed, the spreading thickness of cultivation matrix is 15cm;
3)Tissue-cultured seedling is taken, the culture medium of tissue-cultured seedling root carrying is removed using clear water, after cleaning, the root of tissue-cultured seedling is put Enter in pretreatment fluid, soak 20h, take out, obtain pretreatment tissue-cultured seedling, the pretreatment fluid uses thick wood-fern rhizome and chitosan oligosaccharide system Into;
The preparation method of the pretreatment fluid is as follows:Thick wood-fern rhizome is taken, is cleaned, drying, the ethanol water of 8 times of weight of addition, 2h, heating and refluxing extraction 50min are soaked, obtains reflux extracting liquid and refluxing extraction residue, 5 times are added into refluxing extraction residue The ethanol water of weight, soaks 1h, ultrasonic wave extraction 40min, obtains ultrasonic extract and ultrasonic wave extraction residue, will return Flow extracting solution and ultrasonic extract merges, reduction vaporization concentration is the 10% of original volume, stands 24h, filters out sediment, then The chitosan oligosaccharide of 50g/L is added, after being uniformly mixed, you can obtain pretreatment fluid, the bath temperature of the ultrasonic extraction is 45 DEG C, the frequency of ultrasonic extraction is 50kHz, and the concentration of alcohol in the ethanol water is 60%;
4)It will pre-process on tissue culture transplantation of seedlings to seedbed, and be planted at 25-30 DEG C, watered into seedbed, ensure in seedbed The humidity of cultivation matrix is 35-40%, and humidifier is placed in greenhouse, ensures that the air humidity in greenhouse is 60-70%;
5)After transplanting 20 days, an amino acid chelate fertilizer was sprayed every 7 days, and carried out using carbendazim thimerosal every 15 days Disinfection is once;
6)After 2-3 is planted, you can harvesting.
Embodiment 2
A kind of implantation methods of dendrobium candidum, step are as follows:
1)Prepare cultivation matrix, the cultivation matrix is made of the raw material below according to parts by weight:76 parts of peanut shell, pear tree skin 203 parts, 40 parts of maize cob meal, 55 parts of rice husk, 30 parts of cow dung, 10 parts of humic acid, 16 parts of shell, 6 parts of urea, 3 parts of ammonium phosphate, sulphur Sour 1.5 parts of potassium, 1.2 parts of ammonium molybdate, play both sides of the street 6 parts;
The preparation method of the cultivation matrix is as follows:
a)Take and play both sides of the street, clean, drying, adds the ethanol water of 16 times of weight, soak 50h, then carry out group at room temperature Broken extraction is knitted, the time of historrhexis's extraction is 7min, filtering, obtains historrhexis's extraction filtrate and historrhexis's extraction filter Historrhexis's extraction filter residue, is added the ethanol water of 8 times of weight, soaks 2.5h, be sent into processor for ultrasonic wave by slag, ultrasound 52min is extracted, filtering, obtains ultrasonic extraction liquid and ultrasonic extraction residue, the ultrasonic extraction residue to play both sides of the street is spare, then by group To knit broken extraction filtrate and ultrasonic extraction liquid merges, negative pressure evaporation concentration is the 10% of cumulative volume, stands 24h, filters out sediment, Up to Fructus Lipuidambaris extract, wherein, the bath temperature of the ultrasonic extraction is 50 DEG C, and the frequency of ultrasonic extraction is 30kHz, described Concentration of alcohol in ethanol water is 50%;
b)Peanut shell, pear tree skin, maize cob meal and rice husk are weighed, after merging, is put into mixer, adds gross weight 35% Water, after being uniformly mixed, obtains mixture A;
c)Cow dung and shell are weighed, cow dung is dried 3 days, after shell is crushed, adds in the cow dung to after drying, is stirred After uniformly, mixture B is obtained;
d)Mixture A and mixture B is merged, after being uniformly mixed, piles height 1m, the material heap of width 2m, and expecting heap Mulch in upper cover, carries out spontaneous fermentation;
e)After expecting that heap temperature reaches 55 DEG C, mulch is raised, admixes humic acid and the ultrasonic extraction residue to play both sides of the street, after mixing thoroughly, Mulch is covered, after continuing fermentation 3 days, mulch is raised, obtains fermentation material;
f)Urea, ammonium phosphate, potassium sulfate and ammonium molybdate are weighed, by Fructus Lipuidambaris extract, urea, ammonium phosphate, potassium sulfate and molybdic acid Ammonium is added into fermentation material, after being uniformly mixed, is sent into high voltage sterilizing device, is gone out using vapor under 200 kPas Bacterium handles 2.5h, after natural cooling, up to cultivation matrix;
2)In greenhouse, seedbed is built using hollow brick, seedbed is 53cm apart from ground level, and seedbed width is 1.9m, will be planted Matrix spreading is trained in seedbed, the spreading thickness of cultivation matrix is 16cm;
3)Tissue-cultured seedling is taken, the culture medium of tissue-cultured seedling root carrying is removed using clear water, after cleaning, the root of tissue-cultured seedling is put Enter in pretreatment fluid, soak 22h, take out, obtain pretreatment tissue-cultured seedling, the pretreatment fluid uses thick wood-fern rhizome and chitosan oligosaccharide system Into;
The preparation method of the pretreatment fluid is as follows:Thick wood-fern rhizome is taken, is cleaned, drying, the ethanol water of 8 times of weight of addition, 2h, heating and refluxing extraction 55min are soaked, obtains reflux extracting liquid and refluxing extraction residue, 5 times are added into refluxing extraction residue The ethanol water of weight, soaks 1.5h, ultrasonic wave extraction 43min, obtains ultrasonic extract and ultrasonic wave extraction residue, will Reflux extracting liquid and ultrasonic extract merge, and reduction vaporization concentration is the 10% of original volume, stands 24h, filters out sediment, so The chitosan oligosaccharide of 50g/L is added afterwards, after being uniformly mixed, you can obtain pretreatment fluid, the bath temperature of the ultrasonic extraction is 48 DEG C, the frequency of ultrasonic extraction is 50kHz, and the concentration of alcohol in the ethanol water is 60%;
4)It will pre-process on tissue culture transplantation of seedlings to seedbed, and be planted at 25-30 DEG C, watered into seedbed, ensure in seedbed The humidity of cultivation matrix is 35-40%, and humidifier is placed in greenhouse, ensures that the air humidity in greenhouse is 60-70%;
5)After transplanting 20 days, an amino acid chelate fertilizer was sprayed every 7 days, and carried out using carbendazim thimerosal every 15 days Disinfection is once;
6)After 2-3 is planted, you can harvesting.
Embodiment 3
A kind of implantation methods of dendrobium candidum, step are as follows:
1)Prepare cultivation matrix, the cultivation matrix is made of the raw material below according to parts by weight:78 parts of peanut shell, pear tree skin 205 parts, 43 parts of maize cob meal, 55 parts of rice husk, 27 parts of cow dung, 10 parts of humic acid, 18 parts of shell, 8 parts of urea, 3 parts of ammonium phosphate, sulphur Sour 2 parts of potassium, 1.5 parts of ammonium molybdate, play both sides of the street 7 parts;
The preparation method of the cultivation matrix is as follows:
a)Take and play both sides of the street, clean, drying, adds the ethanol water of 18 times of weight, soak 52h, then carry out group at room temperature Broken extraction is knitted, the time of historrhexis's extraction is 7min, filtering, obtains historrhexis's extraction filtrate and historrhexis's extraction filter Historrhexis's extraction filter residue, is added the ethanol water of 9 times of weight, soaks 2.5h, be sent into processor for ultrasonic wave by slag, ultrasound 55min is extracted, filtering, obtains ultrasonic extraction liquid and ultrasonic extraction residue, the ultrasonic extraction residue to play both sides of the street is spare, then by group To knit broken extraction filtrate and ultrasonic extraction liquid merges, negative pressure evaporation concentration is the 10% of cumulative volume, stands 24h, filters out sediment, Up to Fructus Lipuidambaris extract, wherein, the bath temperature of the ultrasonic extraction is 52 DEG C, and the frequency of ultrasonic extraction is 35kHz, described Concentration of alcohol in ethanol water is 50%;
b)Peanut shell, pear tree skin, maize cob meal and rice husk are weighed, after merging, is put into mixer, adds gross weight 38% Water, after being uniformly mixed, obtains mixture A;
c)Cow dung and shell are weighed, cow dung is dried 4 days, after shell is crushed, adds in the cow dung to after drying, is stirred After uniformly, mixture B is obtained;
d)Mixture A and mixture B is merged, after being uniformly mixed, piles height 1m, the material heap of width 2m, and expecting heap Mulch in upper cover, carries out spontaneous fermentation;
e)After expecting that heap temperature reaches 55 DEG C, mulch is raised, admixes humic acid and the ultrasonic extraction residue to play both sides of the street, after mixing thoroughly, Mulch is covered, after continuing fermentation 4 days, mulch is raised, obtains fermentation material;
f)Urea, ammonium phosphate, potassium sulfate and ammonium molybdate are weighed, by Fructus Lipuidambaris extract, urea, ammonium phosphate, potassium sulfate and molybdic acid Ammonium is added into fermentation material, after being uniformly mixed, is sent into high voltage sterilizing device, is gone out using vapor under 200 kPas Bacterium handles 2.5h, after natural cooling, up to cultivation matrix;
2)In greenhouse, seedbed is built using hollow brick, seedbed is 55cm apart from ground level, and seedbed width is 2m, will be cultivated For matrix spreading in seedbed, the spreading thickness of cultivation matrix is 17cm;
3)Tissue-cultured seedling is taken, the culture medium of tissue-cultured seedling root carrying is removed using clear water, after cleaning, the root of tissue-cultured seedling is put Enter in pretreatment fluid, soak 22h, take out, obtain pretreatment tissue-cultured seedling, the pretreatment fluid uses thick wood-fern rhizome and chitosan oligosaccharide system Into;
The preparation method of the pretreatment fluid is as follows:Thick wood-fern rhizome is taken, is cleaned, drying, the ethanol water of 9 times of weight of addition, 2.5h, heating and refluxing extraction 55min are soaked, reflux extracting liquid and refluxing extraction residue is obtained, 6 is added into refluxing extraction residue The ethanol water of times weight, soaks 1.5h, ultrasonic wave extraction 45min, obtains ultrasonic extract and ultrasonic wave extraction residue, Reflux extracting liquid and ultrasonic extract are merged, reduction vaporization concentration is the 10% of original volume, stands 24h, filters out sediment, Then the chitosan oligosaccharide of 50g/L is added, after being uniformly mixed, you can obtain pretreatment fluid, the bath temperature of the ultrasonic extraction For 48 DEG C, the frequency of ultrasonic extraction is 55kHz, and the concentration of alcohol in the ethanol water is 60%;
4)It will pre-process on tissue culture transplantation of seedlings to seedbed, and be planted at 25-30 DEG C, watered into seedbed, ensure in seedbed The humidity of cultivation matrix is 35-40%, and humidifier is placed in greenhouse, ensures that the air humidity in greenhouse is 60-70%;
5)After transplanting 20 days, an amino acid chelate fertilizer was sprayed every 7 days, and carried out using carbendazim thimerosal every 15 days Disinfection is once;
6)After 2-3 is planted, you can harvesting.
Embodiment 4
A kind of implantation methods of dendrobium candidum, step are as follows:
1)Prepare cultivation matrix, the cultivation matrix is made of the raw material below according to parts by weight:80 parts of peanut shell, pear tree skin 205 parts, 40 parts of maize cob meal, 58 parts of rice husk, 28 parts of cow dung, 12 parts of humic acid, 15 parts of shell, 9 parts of urea, 4 parts of ammonium phosphate, sulphur Sour 2 parts of potassium, 1.5 parts of ammonium molybdate, play both sides of the street 7 parts;
The preparation method of the cultivation matrix is as follows:
a)Take and play both sides of the street, clean, drying, adds the ethanol water of 18 times of weight, soak 55h, then carry out group at room temperature Broken extraction is knitted, the time of historrhexis's extraction is 7min, filtering, obtains historrhexis's extraction filtrate and historrhexis's extraction filter Historrhexis's extraction filter residue, is added the ethanol water of 10 times of weight, soaks 2.5h, be sent into processor for ultrasonic wave, surpass by slag Sound extracts 57min, and filtering, obtains ultrasonic extraction liquid and ultrasonic extraction residue, and the ultrasonic extraction residue to play both sides of the street is spare, then will Historrhexis extracts filtrate and ultrasonic extraction liquid merges, and negative pressure evaporation concentration is the 10% of cumulative volume, stands 24h, filters out precipitation Thing, up to Fructus Lipuidambaris extract, wherein, the bath temperature of the ultrasonic extraction is 55 DEG C, and the frequency of ultrasonic extraction is 40kHz, Concentration of alcohol in the ethanol water is 50%;
b)Peanut shell, pear tree skin, maize cob meal and rice husk are weighed, after merging, is put into mixer, adds gross weight 40% Water, after being uniformly mixed, obtains mixture A;
c)Cow dung and shell are weighed, cow dung is dried 4 days, after shell is crushed, adds in the cow dung to after drying, is stirred After uniformly, mixture B is obtained;
d)Mixture A and mixture B is merged, after being uniformly mixed, piles height 1m, the material heap of width 2m, and expecting heap Mulch in upper cover, carries out spontaneous fermentation;
e)After expecting that heap temperature reaches 55 DEG C, mulch is raised, admixes humic acid and the ultrasonic extraction residue to play both sides of the street, after mixing thoroughly, Mulch is covered, after continuing fermentation 3 days, mulch is raised, obtains fermentation material;
f)Urea, ammonium phosphate, potassium sulfate and ammonium molybdate are weighed, by Fructus Lipuidambaris extract, urea, ammonium phosphate, potassium sulfate and molybdic acid Ammonium is added into fermentation material, after being uniformly mixed, is sent into high voltage sterilizing device, is gone out using vapor under 200 kPas Bacterium handles 2.5h, after natural cooling, up to cultivation matrix;
2)In greenhouse, seedbed is built using hollow brick, seedbed is 60cm apart from ground level, and seedbed width is 2.1m, will be planted Matrix spreading is trained in seedbed, the spreading thickness of cultivation matrix is 18cm;
3)Tissue-cultured seedling is taken, the culture medium of tissue-cultured seedling root carrying is removed using clear water, after cleaning, the root of tissue-cultured seedling is put Enter in pretreatment fluid, soak 23h, take out, obtain pretreatment tissue-cultured seedling, the pretreatment fluid uses thick wood-fern rhizome and chitosan oligosaccharide system Into;
The preparation method of the pretreatment fluid is as follows:Thick wood-fern rhizome is taken, is cleaned, drying, the ethanol water of 9 times of weight of addition, 3h, heating and refluxing extraction 60min are soaked, obtains reflux extracting liquid and refluxing extraction residue, 6 times are added into refluxing extraction residue The ethanol water of weight, soaks 1.5h, ultrasonic wave extraction 50min, obtains ultrasonic extract and ultrasonic wave extraction residue, will Reflux extracting liquid and ultrasonic extract merge, and reduction vaporization concentration is the 10% of original volume, stands 24h, filters out sediment, so The chitosan oligosaccharide of 50g/L is added afterwards, after being uniformly mixed, you can obtain pretreatment fluid, the bath temperature of the ultrasonic extraction is 50 DEG C, the frequency of ultrasonic extraction is 60kHz, and the concentration of alcohol in the ethanol water is 60%;
4)It will pre-process on tissue culture transplantation of seedlings to seedbed, and be planted at 25-30 DEG C, watered into seedbed, ensure in seedbed The humidity of cultivation matrix is 35-40%, and humidifier is placed in greenhouse, ensures that the air humidity in greenhouse is 60-70%;
5)After transplanting 20 days, an amino acid chelate fertilizer was sprayed every 7 days, and carried out using carbendazim thimerosal every 15 days Disinfection is once;
6)After 2-3 is planted, you can harvesting.
Embodiment 5
A kind of implantation methods of dendrobium candidum, step are as follows:
1)Prepare cultivation matrix, the cultivation matrix is made of the raw material below according to parts by weight:80 parts of peanut shell, pear tree skin 210 parts, 45 parts of maize cob meal, 58 parts of rice husk, 30 parts of cow dung, 12 parts of humic acid, 20 parts of shell, 10 parts of urea, 5 parts of ammonium phosphate, 3 parts of potassium sulfate, 2 parts of ammonium molybdate, play both sides of the street 9 parts;
The preparation method of the cultivation matrix is as follows:
a)Take and play both sides of the street, clean, drying, adds the ethanol water of 20 times of weight, soak 60h, then carry out group at room temperature Broken extraction is knitted, the time of historrhexis's extraction is 8min, filtering, obtains historrhexis's extraction filtrate and historrhexis's extraction filter Historrhexis's extraction filter residue, is added the ethanol water of 10 times of weight, soaks 3h, be sent into processor for ultrasonic wave by slag, ultrasound 60min is extracted, filtering, obtains ultrasonic extraction liquid and ultrasonic extraction residue, the ultrasonic extraction residue to play both sides of the street is spare, then by group To knit broken extraction filtrate and ultrasonic extraction liquid merges, negative pressure evaporation concentration is the 10% of cumulative volume, stands 24h, filters out sediment, Up to Fructus Lipuidambaris extract, wherein, the bath temperature of the ultrasonic extraction is 55 DEG C, and the frequency of ultrasonic extraction is 40kHz, described Concentration of alcohol in ethanol water is 50%;
b)Peanut shell, pear tree skin, maize cob meal and rice husk are weighed, after merging, is put into mixer, adds gross weight 40% Water, after being uniformly mixed, obtains mixture A;
c)Cow dung and shell are weighed, cow dung is dried 5 days, after shell is crushed, adds in the cow dung to after drying, is stirred After uniformly, mixture B is obtained;
d)Mixture A and mixture B is merged, after being uniformly mixed, piles height 1m, the material heap of width 2m, and expecting heap Mulch in upper cover, carries out spontaneous fermentation;
e)After expecting that heap temperature reaches 55 DEG C, mulch is raised, admixes humic acid and the ultrasonic extraction residue to play both sides of the street, after mixing thoroughly, Mulch is covered, after continuing fermentation 4 days, mulch is raised, obtains fermentation material;
f)Urea, ammonium phosphate, potassium sulfate and ammonium molybdate are weighed, by Fructus Lipuidambaris extract, urea, ammonium phosphate, potassium sulfate and molybdic acid Ammonium is added into fermentation material, after being uniformly mixed, is sent into high voltage sterilizing device, is gone out using vapor under 200 kPas Bacterium handles 3h, after natural cooling, up to cultivation matrix;
2)In greenhouse, seedbed is built using hollow brick, seedbed is 60cm apart from ground level, and seedbed width is 2.2m, will be planted Matrix spreading is trained in seedbed, the spreading thickness of cultivation matrix is 20cm;
3)Tissue-cultured seedling is taken, the culture medium of tissue-cultured seedling root carrying is removed using clear water, after cleaning, the root of tissue-cultured seedling is put Enter in pretreatment fluid, soak 24h, take out, obtain pretreatment tissue-cultured seedling, the pretreatment fluid uses thick wood-fern rhizome and chitosan oligosaccharide system Into;
The preparation method of the pretreatment fluid is as follows:Thick wood-fern rhizome is taken, is cleaned, drying, the ethanol water of 10 times of weight of addition, 3h, heating and refluxing extraction 60min are soaked, obtains reflux extracting liquid and refluxing extraction residue, 6 times are added into refluxing extraction residue The ethanol water of weight, soaks 2h, ultrasonic wave extraction 50min, obtains ultrasonic extract and ultrasonic wave extraction residue, will return Flow extracting solution and ultrasonic extract merges, reduction vaporization concentration is the 10% of original volume, stands 24h, filters out sediment, then The chitosan oligosaccharide of 50g/L is added, after being uniformly mixed, you can obtain pretreatment fluid, the bath temperature of the ultrasonic extraction is 50 DEG C, the frequency of ultrasonic extraction is 60kHz, and the concentration of alcohol in the ethanol water is 60%;
4)It will pre-process on tissue culture transplantation of seedlings to seedbed, and be planted at 25-30 DEG C, watered into seedbed, ensure in seedbed The humidity of cultivation matrix is 35-40%, and humidifier is placed in greenhouse, ensures that the air humidity in greenhouse is 60-70%;
5)After transplanting 20 days, an amino acid chelate fertilizer was sprayed every 7 days, and carried out using carbendazim thimerosal every 15 days Disinfection is once;
6)After 2-3 is planted, you can harvesting.
Comparative example 1
Compared with Example 3, without playing both sides of the street in its cultivation matrix, other are same as Example 3.
Comparative example 2
Compared with Example 3, tissue-cultured seedling is not placed into pretreatment fluid after cleaning and handled, but is directly transplanted extremely On seedbed, other are same as Example 3.
Planting experiment is carried out using embodiment 1-5 and comparative example 1-2, test method is as follows:
70 cells are taken, each plot area is 3 square metres, is randomly divided into 7 groups, every group of 10 cells, this 7 groups of cells are adopted respectively Dendrobium candidum to be planted with embodiment 1-5 and comparative example 1-2, repeats to test three times, other management measures are all identical, Survival rate, growing way situation and the yield of candidum tissue culturing seedling are observed, and calculates average value.
Result of the test is as follows:
Group Tissue culture shoot survival percent(%) Yield(kg) Plant height(cm)
Embodiment 1 98.6 1.45 35.1
Embodiment 2 98.9 1.50 35.5
Embodiment 3 99.3 1.53 35.9
Embodiment 4 99.1 1.51 35.6
Embodiment 5 98.5 1.45 35.6
Comparative example 1 97.6 1.23 29.3
Comparative example 2 89.3 1.37 33.8
As can be seen from the above results, the implantation methods of dendrobium candidum of the present invention, it is possible to increase the survival rate of tissue-cultured seedling is high, makes iron Skin stem of noble dendrobium growing way is more preferable, improves the yield of dendrobium candidum.
In addition, from embodiment 3 is compared with comparative example 1, the data of comparative example 2 as can be seen that the present invention by using containing The cultivation matrix to play both sides of the street, is conducive to improve the plant height and yield of dendrobium candidum, the present invention in tissue-cultured seedling in cleaning by finishing After be put into pretreatment fluid and handled, be conducive to improve the survival rate of tissue-cultured seedling.
The better embodiment of the present invention is explained in detail above, but the present invention is not limited to above-mentioned embodiment party Formula, can also be on the premise of present inventive concept not be departed from the knowledge that one skilled in the relevant art possesses Various changes can be made.

Claims (7)

1. a kind of implantation methods of dendrobium candidum, it is characterised in that step is as follows:
1)Prepare cultivation matrix, the cultivation matrix is made of the raw material below according to parts by weight:75-80 parts of peanut shell, pear tree 200-210 parts of skin, 40-45 parts of maize cob meal, 52-58 parts of rice husk, 25-30 parts of cow dung, 8-12 parts of humic acid, 15-20 parts of shell, 6-10 parts of urea, 2-5 parts of ammonium phosphate, 1-3 parts of potassium sulfate, 1-2 parts of ammonium molybdate, play both sides of the street 5-9 parts;
2)In greenhouse, seedbed is built using hollow brick, seedbed is 50-60cm apart from ground level, and seedbed width is 1.8- 2.2m, by cultivation matrix spreading in seedbed, the spreading thickness of cultivation matrix is 15-20cm;
3)Tissue-cultured seedling is taken, the culture medium of tissue-cultured seedling root carrying is removed using clear water, after cleaning, the root of tissue-cultured seedling is put Enter in pretreatment fluid, soak 20-24h, take out, obtain pretreatment tissue-cultured seedling, the pretreatment fluid is few using thick wood-fern rhizome and shell Sugar is made;
4)It will pre-process on tissue culture transplantation of seedlings to seedbed, and be planted at 25-30 DEG C, watered into seedbed, ensure in seedbed The humidity of cultivation matrix is 35-40%, and humidifier is placed in greenhouse, ensures that the air humidity in greenhouse is 60-70%;
5)After transplanting 20 days, an amino acid chelate fertilizer was sprayed every 7 days, and carried out using carbendazim thimerosal every 15 days Disinfection is once;
6)After 2-3 is planted, you can harvesting.
2. the implantation methods of dendrobium candidum according to claim 1, it is characterised in that the preparation method of the cultivation matrix It is as follows:
a)Take and play both sides of the street, clean, drying, adds the ethanol water of 15-20 times of weight, soak 48-60h, then at room temperature Historrhexis's extraction is carried out, the time of historrhexis's extraction is 6-8min, filtering, obtains historrhexis's extraction filtrate and tissue is broken Historrhexis's extraction filter residue, is added the ethanol water of 8-10 times of weight, soaks 2-3h, be sent at ultrasonic wave by broken extraction filter residue Manage in device, ultrasonic extraction 50-60min, filtering, obtain ultrasonic extraction liquid and ultrasonic extraction residue, the ultrasonic extraction to play both sides of the street are residual Slag is spare, and historrhexis then is extracted filtrate and ultrasonic extraction liquid merges, negative pressure evaporation concentration is the 10% of cumulative volume, is stood 24h, filters out sediment, up to Fructus Lipuidambaris extract;
b)Peanut shell, pear tree skin, maize cob meal and rice husk are weighed, after merging, is put into mixer, adds gross weight 35-40% Water, after being uniformly mixed, obtain mixture A;
c)Cow dung and shell are weighed, cow dung is dried 3-5 days, after shell is crushed, is added in the cow dung to after drying, stirring is mixed After closing uniformly, mixture B is obtained;
d)Mixture A and mixture B is merged, after being uniformly mixed, piles height 1m, the material heap of width 2m, and expecting heap Mulch in upper cover, carries out spontaneous fermentation;
e)After expecting that heap temperature reaches 55 DEG C, mulch is raised, admixes humic acid and the ultrasonic extraction residue to play both sides of the street, after mixing thoroughly, Mulch is covered, after continuing fermentation 3-4 days, mulch is raised, obtains fermentation material;
f)Urea, ammonium phosphate, potassium sulfate and ammonium molybdate are weighed, by Fructus Lipuidambaris extract, urea, ammonium phosphate, potassium sulfate and molybdic acid Ammonium is added into fermentation material, after being uniformly mixed, is sent into high voltage sterilizing device, is gone out using vapor under 200 kPas Bacterium handles 2-3h, after natural cooling, up to cultivation matrix.
3. the implantation methods of dendrobium candidum according to claim 2, it is characterised in that step a)In, the ultrasonic extraction Bath temperature be 50-55 DEG C, the frequency of ultrasonic extraction is 30-40kHz.
4. the implantation methods of dendrobium candidum according to claim 2, it is characterised in that the ethanol in the ethanol water Concentration is 50%.
5. according to the implantation methods of any dendrobium candidums of claim 1-4, it is characterised in that the system of the pretreatment fluid Preparation Method is as follows:Thick wood-fern rhizome is taken, is cleaned, drying, adds the ethanol water of 8-10 times of weight, soak 2-3h, be heated to reflux 50-60min is extracted, obtains reflux extracting liquid and refluxing extraction residue, the ethanol of 5-6 times of weight is added into refluxing extraction residue Aqueous solution, soaks 1-2h, ultrasonic wave extraction 40-50min, obtains ultrasonic extract and ultrasonic wave extraction residue, reflux is carried Liquid and ultrasonic extract is taken to merge, reduction vaporization concentration is the 10% of original volume, stands 24h, filters out sediment, then add The chitosan oligosaccharide of 50g/L, after being uniformly mixed, you can obtain pretreatment fluid.
6. the implantation methods of dendrobium candidum according to claim 5, it is characterised in that the bath temperature of the ultrasonic extraction For 45-50 DEG C, the frequency of ultrasonic extraction is 50-60kHz.
7. the implantation methods of dendrobium candidum according to claim 5, it is characterised in that the ethanol in the ethanol water Concentration is 60%.
CN201711257538.0A 2017-12-04 2017-12-04 A kind of implantation methods of dendrobium candidum Pending CN107980496A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110710438A (en) * 2018-07-13 2020-01-21 贵州元亨利农业科技开发有限公司 Sandy loam planted by traditional Chinese medicinal materials and preparation method thereof

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103918536A (en) * 2014-04-30 2014-07-16 成都润惠农业开发有限公司 Planting method for Dendrobium officinale
CN104322257A (en) * 2014-10-29 2015-02-04 安徽牧龙山生态旅游开发股份有限公司 Method for normatively planting dendrobium officinale in simple greenhouse
CN104522089A (en) * 2015-01-13 2015-04-22 姬俊杰 Green pollution-free plant compound preparation for preventing and treating virus, fungal and bacterial diseases of crops and preparation method and application thereof
CN104813908A (en) * 2015-04-24 2015-08-05 广西经正科技开发有限责任公司 Dendrobium officinale cultivation grain substrate and preparation method thereof
CN106613832A (en) * 2016-11-17 2017-05-10 贵州绿健神农有机农业股份有限公司 Culture method of dendrobium officinale
CN107027579A (en) * 2017-05-10 2017-08-11 麦丽 A kind of method of peach nursery cutting propagation

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103918536A (en) * 2014-04-30 2014-07-16 成都润惠农业开发有限公司 Planting method for Dendrobium officinale
CN104322257A (en) * 2014-10-29 2015-02-04 安徽牧龙山生态旅游开发股份有限公司 Method for normatively planting dendrobium officinale in simple greenhouse
CN104522089A (en) * 2015-01-13 2015-04-22 姬俊杰 Green pollution-free plant compound preparation for preventing and treating virus, fungal and bacterial diseases of crops and preparation method and application thereof
CN104813908A (en) * 2015-04-24 2015-08-05 广西经正科技开发有限责任公司 Dendrobium officinale cultivation grain substrate and preparation method thereof
CN106613832A (en) * 2016-11-17 2017-05-10 贵州绿健神农有机农业股份有限公司 Culture method of dendrobium officinale
CN107027579A (en) * 2017-05-10 2017-08-11 麦丽 A kind of method of peach nursery cutting propagation

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110710438A (en) * 2018-07-13 2020-01-21 贵州元亨利农业科技开发有限公司 Sandy loam planted by traditional Chinese medicinal materials and preparation method thereof

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