CN107912526A - A kind of preparation method and application of nitric oxide donator type chitosan nano antistaling agent - Google Patents

A kind of preparation method and application of nitric oxide donator type chitosan nano antistaling agent Download PDF

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Publication number
CN107912526A
CN107912526A CN201711374010.1A CN201711374010A CN107912526A CN 107912526 A CN107912526 A CN 107912526A CN 201711374010 A CN201711374010 A CN 201711374010A CN 107912526 A CN107912526 A CN 107912526A
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CN
China
Prior art keywords
parts
solution
antistaling agent
nitric oxide
chitosan nano
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201711374010.1A
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Chinese (zh)
Inventor
朱树华
马媛媛
黄丹丹
周杰
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Shandong Agricultural University
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Shandong Agricultural University
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Publication date
Priority to CN201711188344X priority Critical
Priority to CN201711188344 priority
Application filed by Shandong Agricultural University filed Critical Shandong Agricultural University
Publication of CN107912526A publication Critical patent/CN107912526A/en
Pending legal-status Critical Current

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Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B7/00Preservation or chemical ripening of fruit or vegetables
    • A23B7/14Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
    • A23B7/153Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
    • A23B7/154Organic compounds; Microorganisms; Enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B7/00Preservation or chemical ripening of fruit or vegetables
    • A23B7/14Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
    • A23B7/153Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
    • A23B7/157Inorganic compounds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

The present invention relates to a kind of preparation method of nitric oxide donator type chitosan nano antistaling agent, its component and its mass fraction are:3 12 parts of chitosan, 10 15 parts of glutathione, 10 20 parts of organic acid, 0.5 1 parts of sodium tripolyphosphate, 1.5 3.5 parts of sodium nitrite, 4000 parts of water;Nitric oxide donator type chitosan nano antistaling agent prepared by the present invention contains chitosan and releasable nitric oxide production glutathione carrier components, chitosan is combined to act on nitric oxide production dual fresh-keeping, more preferable fresh-keeping effect can be reached, can significantly delay the dehydration process, softening process and aging course of fruits and vegetables;The green non-poisonous edible of this antistaling agent at the same time, and preparation process is simple, can be more convenient and widely applied, there is very big application potential in preserving fruit and vegetable utilizing field.

Description

A kind of preparation method and application of nitric oxide donator type chitosan nano antistaling agent
Technical field
The invention belongs to postharvest technology of fruits and vegetables field, is related to a kind of system of nitric oxide donator type chitosan nano antistaling agent Preparation Method and application.
Background technology
Fruits and vegetables are front and rear due to reasons such as physiological aging and mechanical damages in picking, easily decline storage quality, or even rot It is rotten, influence economic well-being of workers and staff.The common measure for slowing down fruit vegetables storing quality decline at present is cryopreservation and combines chemistry guarantor The regulatory factor of fresh processing, wherein nitric oxide as fruit maturation, can suppress fruit maturation and aging.Substantial amounts of research The verified nitric oxide with appropriate concentration handles fruit, can be generated with ethene suppressing, extend the storage time of post-harvest fruits. At present, the processing mode in terms of nitric oxide is applied to fresh preservation is more using gas fumigation or solution immersion etc., due to an oxygen It is gas to change nitrogen, its storage and transport and use condition are harsher, more inconvenient in the application of fresh-keeping industry.Therefore can Slowly the donator type antistaling agent of release nitric oxide gas is urgently studied.
The content of the invention
To solve the above-mentioned problems, the present invention provides a kind of nitric oxide donator type chitosan nano antistaling agent;Shell gathers Sugared (Chitosan (1-4) -2- amino-beta-D glucose) is a kind of Natural linear cation life of rich content in nature Thing polymer, containing the hydrophilic radical such as substantial amounts of hydroxyl and amino, due to it is of low cost, be readily biodegradable and good film-forming property, Respiratory intensity etc. can be suppressed growth of microorganism and suppress by being attached to fruit and vegetable surfaces;The present invention by nanometer technology by chitosan with Nitric oxide donors combine, and are acted on, can formed a film antibacterial using chitosan and nitric oxide production dual fresh-keeping It can suppress breathing and ethylene synthesis again, more effective fresh-keeping effect can be reached.
A kind of nitric oxide donator type chitosan nano antistaling agent, its component and its mass fraction are:3-12 parts of chitosan, 10-15 parts of glutathione, 10-20 parts of organic acid, 0.5-1 parts of sodium tripolyphosphate, 1.5-3.5 parts of sodium nitrite, 4000 parts of water.
The organic acid is citric acid, acetic acid, malic acid or lactic acid.
Present invention also offers a kind of preparation method of nitric oxide donator type chitosan nano antistaling agent, preparation process is such as Under:
(1) 10-20 parts of organic acid, 3-12 parts of chitosan are added sequentially in 1000 parts of water, are uniformly mixing to obtain solution 1。
(2) by glutathione 10-15 parts be added in 1000 parts of water, 0.5-1 parts of sodium tripolyphosphate is added to 1000 parts of water In, 1.5-3.5 parts of sodium nitrite is added in 1000 parts of water, is stirred evenly and is respectively obtained solution 2, solution 3, solution 4.
(3) solution 2 is added in solution 1, stirring obtains solution 5 in 90 minutes.
(4) solution 3 is added dropwise in solution 5, is uniformly mixing to obtain solution 6.
(5) solution 4 is added in solution 6, lucifuge stirs 45 minutes, that is, chitosan nano antistaling agent is made.
The immersion of nitric oxide donator type chitosan nano antistaling agent, smearing or sprinkling fruits and vegetables prepared by the present invention, dry Stored afterwards under room temperature or low temperature, can significantly delay the dehydration process, softening process and aging course of fruits and vegetables.
Compared with existing fruit and vegetable fresh-keeping agent, the nitric oxide donator type chitosan nano antistaling agent prepared using the present invention is contained There are chitosan and releasable nitric oxide production glutathione carrier components, combine chitosan and nitric oxide production dual fresh-keeping Effect, can reach more preferable fresh-keeping effect, can significantly delay the dehydration process, softening process and aging course of fruits and vegetables;At the same time The green non-poisonous edible of this antistaling agent, and preparation process is simple, can be more convenient and widely applied, protected in fruits and vegetables There is very big application potential in fresh field.
Brief description of the drawings
Fig. 1 is the influence that antistaling agent of the present invention adopts strawberry fruit rear firmness change at room temperature.
From Fig. 1 the change of hardness can be seen that postharvest handling storage time 0-60 it is small when interior, 1 product treatment of embodiment Strawberry fruit hardness decline substantially than unused antistaling agent handle fruit it is slow, illustrate that antistaling agent of the present invention can substantially prolong The softening process of slow strawberry fruit.
Fig. 2 is the influence that antistaling agent of the present invention adopts strawberry fruit rear chromatic aberration at room temperature.
Color scale is lower, and fruit colour is darker, and fruit gets over aging.The change of aberration can be seen that postharvest handling from Fig. 2 Storage time 0-60 is interior when small, and the color scale of strawberry fruit is all into downward trend, the color of the strawberry fruit of 2 product treatment of embodiment The fruit that antistaling agent processing is not used in margin suppression ratio is slow, illustrate antistaling agent of the present invention can obvious delayed fruit aging Journey.
Fig. 3 is the influence that antistaling agent of the present invention adopts cucumber fruits rear percentage of water loss change at room temperature.
The change of percentage of water loss can be seen that in postharvest handling storage time 0-12 days from Fig. 3,2 product treatment of embodiment Cucumber fruits percentage of water loss rise substantially than unused antistaling agent handle fruit it is slow, illustrate that antistaling agent of the present invention can be obvious Delay the dehydration process of cucumber fruits.
Embodiment
The present invention is described in further detail with reference to embodiment, the embodiment provided is only to illustrate the present invention, And do not limit the scope of the invention.All reagents used in embodiment are commercially available, its manufacturer and lot number are as follows:
Chitosan:Purchased from Shanghai Yuan Ye Bioisystech Co., Ltd, lot number:A24M8X36696;
Glutathione:Purchased from Shanghai Yuan Ye Bioisystech Co., Ltd, lot number:Q19M8N36347;
Sodium tripolyphosphate:Purchased from Tianjin Kai Tong chemical reagent Co., Ltd, lot number:20161020;
Acetic acid:Purchased from Tianjin Kai Tong chemical reagent Co., Ltd, lot number:20160616;
Citric acid:Purchased from Tianjin Kai Tong chemical reagent Co., Ltd, lot number:20160815;
Sodium nitrite:Purchased from Tianjin Kai Tong chemical reagent Co., Ltd, lot number:20160428.
Embodiment 1
A kind of nitric oxide donator type chitosan nano antistaling agent, includes following components:Chitosan, glutathione, acetic acid, Sodium tripolyphosphate, sodium nitrite and water.
The preparation method of chitosan nano antistaling agent, comprises the following steps:
(1) 15 parts of acetic acid, 4 parts of chitosan are added sequentially in 1000 parts of water, are uniformly mixing to obtain solution 1.
(2) by glutathione 12 parts be added in 1000 parts of water, 0.6 part of sodium tripolyphosphate is added in 1000 parts of water, sub- 2.8 parts of sodium nitrate is added in 1000 parts of water, is stirred evenly and is respectively obtained solution 2, solution 3, solution 4.
(3) solution 2 is added in solution 1, stirring obtains solution 5 in 90 minutes.
(4) solution 3 is added dropwise in solution 5, is uniformly mixing to obtain solution 6.
(5) solution 4 is added in solution 6, lucifuge stirs 45 minutes, that is, chitosan nano antistaling agent is made.
The chitosan nano antistaling agent of above-mentioned preparation is applied to the fresh-keeping of strawberry fruit, specific method is:Buying is newly adopted The fresh strawberry plucked, subtracts disease, part that is blue or green and having mechanical damage, ensures that fruit is fresh, uniform.By manufactured chitosan nano Rice antistaling agent is directly sprayed on strawberry fruit surface, dries rear room temperature storage.
From Fig. 1 the change of hardness can be seen that postharvest handling storage time 0-60 it is small when interior, shell manufactured in the present embodiment The fruit that the hardness of strawberry fruit after the processing of glycan nanometer fresh-keeping agent declines substantially than the processing of unused antistaling agent is slow, illustrates this Invention antistaling agent can substantially delay the softening process of strawberry fruit.
Meanwhile color scale is lower, fruit colour is darker, and fruit gets over aging.The change of aberration, which can be seen that, from Fig. 2 adopts Post processing storage time 0-60 is interior when small, and the color scale of strawberry fruit is all into downward trend, at chitosan nano antistaling agent The fruit that antistaling agent processing is not used in the color scale suppression ratio of strawberry fruit after reason is slow, illustrates that antistaling agent of the present invention can be obvious The aging course of delayed fruit.
Embodiment 2
A kind of nitric oxide donator type chitosan nano antistaling agent, includes following components:Chitosan, glutathione, lemon Acid, sodium tripolyphosphate, sodium nitrite and water.
The preparation method of chitosan nano antistaling agent, comprises the following steps
(1) 15 parts of citric acid, 4 parts of chitosan are added sequentially in 1000 parts of water, are uniformly mixing to obtain solution 1.
(2) by glutathione 12 parts be added in 1000 parts of water, 0.6 part of sodium tripolyphosphate is added in 1000 parts of water, sub- 2.8 parts of sodium nitrate is added in 1000 parts of water, is stirred evenly and is respectively obtained solution 2, solution 3, solution 4.
(3) solution 2 is added in solution 1, stirring obtains solution 5 in 90 minutes.
(4) solution 3 is added dropwise in solution 5, is uniformly mixing to obtain solution 6.
(5) solution 4 is added in solution 6, lucifuge stirs 45 minutes, that is, chitosan nano antistaling agent is made.
The chitosan nano antistaling agent of above-mentioned preparation is fresh-keeping applied to cucumber fruits, and specific method is:The new picking of buying Fresh cucumber, subtract disease, part that is small and having mechanical damage, ensure that fruit is fresh, uniform.By manufactured chitosan nano Antistaling agent is directly sprayed on cucumber fruits surface, dries rear room temperature storage.
The change of percentage of water loss can be seen that in postharvest handling storage time 0-12 days from Fig. 3, shell manufactured in the present embodiment The fruit that the percentage of water loss of cucumber fruits after the processing of glycan nanometer fresh-keeping agent rises substantially than the processing of unused antistaling agent is slow, explanation Antistaling agent of the present invention can substantially delay the dehydration process of cucumber fruits.

Claims (6)

1. a kind of nitric oxide donator type chitosan nano antistaling agent, it is characterised in that each component and its mass fraction are:Shell gathers It is 3-12 parts sugared, 10-15 parts of glutathione, 10-20 parts of organic acid, 0.5-1 parts of sodium tripolyphosphate, 1.5-3.5 parts of sodium nitrite, water 4000 parts.
2. a kind of nitric oxide donator type chitosan nano antistaling agent according to claim 1, it is characterised in that described Organic acid is citric acid, acetic acid, malic acid or lactic acid.
3. a kind of preparation method of nitric oxide donator type chitosan nano antistaling agent as claimed in claim 1, its feature exist In step is as follows:
1) 10-20 parts of organic acid, 3-12 parts of chitosan are added sequentially in 1000 parts of water, are uniformly mixing to obtain solution 1;
2) by glutathione 10-15 parts be added in 1000 parts of water, 0.5-1 parts of sodium tripolyphosphate is added in 1000 parts of water, sub- 1.5-3.5 parts of sodium nitrate is added in 1000 parts of water, is stirred evenly and is respectively obtained solution 2, solution 3, solution 4;
3) solution 2 is added in solution 1, stirring obtains solution 5 in 90 minutes;
4) solution 3 is added dropwise in solution 5, is uniformly mixing to obtain solution 6;
5) solution 4 is added in solution 6, lucifuge stirs 45 minutes, and chitosan nano antistaling agent is made.
4. a kind of nitric oxide donator type chitosan nano antistaling agent as claimed in claim 1 delay fruits and vegetables dehydration process, Application in softening process and aging course, it is characterised in that fruits and vegetables are soaked, smeared or sprayed with antistaling agent, dry rear room temperature Or stored under low temperature.
5. a kind of nitric oxide donator type chitosan nano antistaling agent as claimed in claim 1 is delaying cucumber fruits dehydration Application in journey.
6. a kind of nitric oxide donator type chitosan nano antistaling agent as claimed in claim 1 is delaying the softening of strawberry fruit Application in process and aging course.
CN201711374010.1A 2017-11-22 2017-12-19 A kind of preparation method and application of nitric oxide donator type chitosan nano antistaling agent Pending CN107912526A (en)

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CN201711188344X 2017-11-22
CN201711188344 2017-11-22

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110800802A (en) * 2019-10-22 2020-02-18 中国药科大学 Sodium alginate coating for optically controlling release of nitric oxide and preparation method and application thereof
WO2020183491A1 (en) * 2019-03-11 2020-09-17 National Institute Of Plant Genome Research Method for extending shelf-life of agricultural produce

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101485355A (en) * 2009-03-02 2009-07-22 邵毅 Antistaling agent for fingered citron fruit and method of use thereof
CN104082636A (en) * 2014-06-11 2014-10-08 南通昊友食品添加剂有限公司 Antioxidant composition
CN106456563A (en) * 2014-02-28 2017-02-22 釜山大学校产学协力团 Nitrogen oxide-releasing wound treatment film and preparation method therefor

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101485355A (en) * 2009-03-02 2009-07-22 邵毅 Antistaling agent for fingered citron fruit and method of use thereof
CN106456563A (en) * 2014-02-28 2017-02-22 釜山大学校产学协力团 Nitrogen oxide-releasing wound treatment film and preparation method therefor
CN104082636A (en) * 2014-06-11 2014-10-08 南通昊友食品添加剂有限公司 Antioxidant composition

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
M. T. PELEGRINO,ETL: ""Chitosan nanoparticles for nitric oxide delivery in human skin"", 《MEDCHEMCOMM》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020183491A1 (en) * 2019-03-11 2020-09-17 National Institute Of Plant Genome Research Method for extending shelf-life of agricultural produce
CN110800802A (en) * 2019-10-22 2020-02-18 中国药科大学 Sodium alginate coating for optically controlling release of nitric oxide and preparation method and application thereof

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