A kind of highly-safe L-ornidazole injection liquid and preparation method thereof
Technical field
The present invention relates to pharmaceutical formulations and preparation method thereof, and in particular to a kind of injection containing laevo-ornidazole
And preparation method thereof.
Background technique
Ornidazole (Ornidazole), entitled 1- (the chloro- 2- hydroxypropyl of the 3-) -2- 5-nitro imidazole of chemistry, molecular formula
For C7H10ClN3O3, indication be by bacteroides fragilis, clostruidium, Eubacterium, peptococcus and peptostreptococcus,
Multi-infection disease caused by the sensitivity anaerobic bacteria such as helicobacter pylori, bacaeroides melaninogenicus, Fusobacterium, gum bacteroid.
Ornidazole is succeeded in developing by U.S. Hoffer.M etc. earliest, then by Roche company in 1977-1983 successively Germany, method
The states such as state, Italy, Switzerland are with " Tiberal " listing.China had approved Ornidazole raw material and each since 2002 in succession
The production of kind preparation.The country has including dosage forms such as tablet, dispersible tablet, capsule, injection, sodium chloride injections at present, wherein
Injection is occupied an leading position.
CN1686116A discloses a kind of intravenous administration formulation of laevo-ornidazole, improves the dissolution of laevo-ornidazole
Degree.
CN1739505A discloses a kind of L-ornidazole injection preparation, has quality stable, controllable, safely and effectively waits spies
Point.
Laevo-ornidazole is the levo form compound of Ornidazole, and Central neurotoxicity is significantly lower than racemic Ornidazole,
It therefore is at present on the market mainly the preparation of laevo-ornidazole.Laevo-ornidazole preparation, especially L-ornidazole sodium chloride note
Liquid is penetrated in production and storage, a variety of related substances can be generated.Generation in relation to substance can not only reduce main content, also
Toxicity can be generated or aggravate adverse reaction.
Forefathers are the study found that the adverse reaction rate of L-ornidazole sodium chloride injection is about 1.5%, including is digested
System adverse reaction is such as had a stomach upset, nervous system adverse reaction is such as dizzy, immune system adverse reaction such as granulocyte is reduced
Symptom.Although the adverse reaction rate than ornidazole sodium chloride injection has been significantly reduced, a wide range of crowd
In in use, the adverse reaction rate of L-ornidazole sodium chloride injection 1.5% can still bring a large amount of adverse reaction patient.
Therefore, the related substance for controlling laevo-ornidazole, reduces its toxicity, improves its safety, preferably plays it and faces
Bed curative effect, becomes the research direction being of practical significance very much.
Summary of the invention
For this purpose, applicant has conducted extensive research laevo-ornidazole and racemic Ornidazole, and it is found surprisingly that, in life
Produce and storage process in, this forefathers of nitrate anion can be generated in a variety of Ornidazole preparations not it has been found that related substance, and it is this
Impurity can not only reduce the drug effect of Ornidazole preparation, can also improve the toxicity of Ornidazole preparation in vivo to a certain extent.Institute
The nitrate anion impurity stated includes the various molecules containing nitrate anion.
Therefore, it is necessary to control the amount in Ornidazole preparation in relation to substance to improve its safety in utilization.
Based on object above, the present invention provides a kind of Ornidazole preparation, often uses auxiliary material, nitrate anion containing Ornidazole, preparation,
Wherein, nitrate radical content is not higher than 30mg/l.Preferably, the ratio between Ornidazole content and nitrate radical content are not less than 150:1.
Further, the present invention provides a kind of highly-safe L-ornidazole injection liquid, including laevo-ornidazole, isotonic
Regulator component, which is characterized in that in the L-ornidazole injection liquid, contain nitrate anion, wherein nitrate radical content is not high
In 30mg/l, preferably no greater than 15mg/l, more preferably no higher than 6mg/l.The ratio between the content of laevo-ornidazole and nitrate anion is not low
In 150:1, the ratio between preferred content is not less than 500:1, and the ratio between preferred content is not less than 1000:1.
In the L-ornidazole injection liquid, laevo-ornidazole content (w/v) is not less than 0.3%, and preferred content is not less than
0.4%, more preferable content is 0.5%.
In the L-ornidazole injection liquid, isotonic regulator constituent content (w/v) is not less than 0.1%, and preferred content is not
Lower than 0.5%, preferred content is 0.83%.
The isotonic regulator be selected from sodium chloride, glucose, fructose, phosphate, citrate, mannitol, polyethylene glycol,
One or more of combinations of propylene glycol.
Further, pharmaceutically acceptable buffer salt can also be contained in the L-ornidazole injection liquid, it is described slow
Rush one or more of combinations that salt is selected from such as citrate, phosphate, carbonate.
Further, pharmaceutically acceptable antioxidant can also be contained in the L-ornidazole injection liquid, it is described
Antioxidant is selected from one or more of combinations of such as cysteine, sodium hydrogensulfite, glycine.
In addition, inventor is found surprisingly that, and when the content of nitrate anion is more than 30mg/l, L-ornidazole sodium chloride injection
The adverse reaction rate of liquid can be significantly increased, specifically, the adverse reaction rate of user's nervous system significantly improves,
The adverse reaction rate of digestive system significantly improves.
The present invention also provides a kind of preparation method of laevo-ornidazole, step includes: in the appearance being protected from light, anaerobic is protected
In device, organic solvent and 2- 5-nitro imidazole is added, catalyst (control temperature is no more than 10 DEG C) is slowly added under stirring,
After being added dropwise, then S- (+)-epoxychloropropane (control temperature is no more than 10 DEG C) is slowly added dropwise, 0-20 DEG C of reaction 2-10 is small
When, reaction solution is slowly added into mixture of ice and water, temperature is no more than 30 DEG C and is stirred to react -5 hours 10 minutes, adjusts pH with acid
Value is 1.0-2.0, divides and removes organic layer, and water layer is 6.0-8.0 with adjusting PH with base, and stirring and crystallizing -24 hours 1 hour, water was used in filtering
Washing, dries to obtain laevo-ornidazole.
In particular the organic solvent being added in above-mentioned steps container are as follows: ethyl acetate, toluene, methylene chloride, second
One or more of combinations of ether, ethyl acetate or methylene chloride.
Catalyst is boron trifluoride in above-mentioned steps, and boron trifluoride can be that gas can also be the organic molten of boron trifluoride
Liquid, preferably boron trifluoride ether solution, boron trifluoride ethyl acetate solution, boron trifluoride dichloromethane solution, boron trifluoride
The one or more of acetonitrile solution, boron trifluoride acetone soln.
It is sulfuric acid, hydrochloric acid or phosphoric acid, preferably hydrochloric acid that acid used in pH is adjusted in above-mentioned steps.
In above-mentioned steps adjust pH used in alkali be inorganic base or organic base, preferably sodium hydroxide, potassium hydroxide, sodium carbonate,
One or more of combinations of potassium carbonate, ammonium hydroxide, triethylamine, diethylamine.
The present invention also provides a kind of method for preparing L-ornidazole injection liquid, step include: (1) be protected from light, nothing
Under conditions of oxygen protection, sodium chloride is weighed, laevo-ornidazole adds water for injection and stirs to dissolve, and benefit injects water to
Close to full dose;(2) activated carbon adsorption 5-60min is added, adjusts pH to 3.0-4.0 with pH adjusting agent after filtering, adds injection
Water is to full dose;(3) filling, it seals after Zha Gai, sterilizes at high temperature.
Preferably, filling preceding 0.45 μm of filtering with microporous membrane.
Preferably, sodium citrate is weighed in step (1) and dissolved together with sodium chloride, laevo-ornidazole, it is furthermore preferred that claiming
Take final 0.003% sodium citrate of injection weight.
The temperature for dissolving sodium chloride and laevo-ornidazole is 30 DEG C -60 DEG C, preferably 45 DEG C -55 DEG C.
Activated carbon dosage is 0.005%-0.015%.
The pH adjusting agent is hydrochloric acid.
The condition of the high-temperature sterilization is the 10min-20min that sterilizes at 110 DEG C -130 DEG C.Preferably, the item of high-temperature sterilization
Part is at 115 DEG C, and sterilize 30min.
The present invention also provides a kind of detection methods of nitrate anion in L-ornidazole injection liquid.This method is suitable for left-handed
The detection of nitrate anion in ornidazole injection, and convenient for operation, separating degree is high, favorable reproducibility.
Nitrate anion in the L-ornidazole injection liquid is detected using liquid chromatography.
Stationary phase is using octadecylsilane chemically bonded silica as filler;Mobile phase is mixed by mobile phase A liquid with acetonitrile,
The two ratio is 70:30-90:10, mobile phase A preparation method are as follows: weigh the potassium dihydrogen phosphate of parts by weight 1-1.5 in parts by weight
It in 1000 ultrapure water, sufficiently dissolves, adds the tetrabutylammonium hydroxide solution of the 10% of parts by weight 20-30, mix, use
20% phosphoric acid tune pH value is filtered to 6.0-8.0 to obtain the final product;Flow velocity is 0.6-1.0ml/min;Column temperature is 20 DEG C -40 DEG C;Detection
Wavelength is 210-230nm;Sample volume is 5-20 μ l;Runing time is 20-40min;It is pair with benchmark grade sodium nitrate when detection
According to product, the content of nitrate anion in ornidazole sodium chloride injection is calculated with external standard method.
The present invention also provides a kind of quality evaluating method of L-ornidazole injection liquid, the method is to detect injection
The content of middle nitrate anion determines that it is rejected product when nitrate radical content is more than 30mg/l in the injection.
The present invention also provides a kind of method for improving L-ornidazole injection liquid safety in utilization, the method is to detect
The content of nitrate anion in injection determines that it is rejected product when nitrate anion amount is more than 30mg/l in the injection, and
It abandons;When nitrate radical content is no more than 30mg/l in the injection, if nitrate radical content is xmg/l, then the left side
Rotation ornidazole injection (30-x) should use in * 1.1 months after sensing.
Compared with prior art, the present invention has the advantage that
1. present invention finds related substances new in a kind of Ornidazole preparation, and adequately grind to its toxicity
Study carefully, while having speculated the relationship between this impurity and Ornidazole adverse reaction.
2. highly-safe, toxicity is low the present invention provides a kind of L-ornidazole injection liquid, a left side can be preferably played
Revolve the clinical efficacy of Ornidazole.
3., to the detection method of nitrate anion, separating degree is high, specificity the present invention provides in L-ornidazole injection liquid
It is good, accurate detection can be carried out to the nitrate anion in laevo-ornidazole.
4. the laevo-ornidazole of this method preparation, purity is more the present invention provides a kind of preparation method of laevo-ornidazole
Height, the related content of material of the preparation of preparation is small, improves the safety of preparation.
5. the present invention also provides a kind of preparation method of L-ornidazole injection liquid, the injection safety of this method preparation
Property it is good, stability is high, and the related content of material of L-ornidazole injection liquid can be effectively reduced.
6. the present invention also provides a kind of methods of L-ornidazole injection liquid quality evaluation, by nitrate radical content
The product that related content of material is higher than standard is determined as rejected product, made to avoid the higher product of toxicity by patient by detection
With to improve the safety in utilization of L-ornidazole injection liquid.
7. the present invention also provides a kind of methods for improving L-ornidazole injection liquid safety in utilization, by nitrate anion
The detection of content, to judge that this batch of product can continue the time saved, to reduce risk when patient medication.
Specific embodiment
Below by embodiment, the present invention is further illustrated.It should be understood that the embodiment of the present invention is only to use
It is rather than limiting the invention, equal to simple modifications of the invention under concept thereof of the invention in illustrating the present invention
Belong to the scope of protection of present invention.
Comparative example 1
L-ornidazole injection liquid is prepared according to the method for CN1686116A, and is repeated 5 times.
Comparative example 2
L-ornidazole injection liquid is prepared according to the method for CN1739505A, and is repeated 5 times.
Comparative example 3
Precision weighs sodium chloride 83g, and laevo-ornidazole 50g adds water for injection 9000ml to stir to dissolve, adds injection
Water is to 10000ml.Add active carbon 10g to adsorb 15min, adjusts pH to 3.60 with dilute hydrochloric acid after filtering.It is filtered with 0.45 μm of micropore
Film filtering, it is filling, it is sealed after Zha Gai, then through 115 DEG C of steam sterilization 30min, obtain finished product, and be repeated 5 times.
Comparative example 4
Precision weighs sodium chloride 83g, laevo-ornidazole 50g, sodium nitrate 0.2g, and water for injection 9000ml stirring is added to keep its molten
Solution, injects water to 10000ml.Add active carbon 10g to adsorb 15min, adjusts pH to 3.60 with dilute hydrochloric acid after filtering.With
0.45 μm of filtering with microporous membrane, it is filling, it is sealed after Zha Gai, then through 115 DEG C of steam sterilization 30min, obtain finished product, and repeat 5
It is secondary.
Embodiment 1
It is being protected from light, in the 50L reaction kettle under oxygen free condition, the 2- methyl-5-nitro miaow of 20L ethyl acetate and 2kg is added
Azoles is cooled to -10 DEG C, and the ethyl acetate solution (the about 1.1kg containing boron trifluoride) that boron trifluoride is slowly added dropwise (controls temperature not
More than 15 DEG C), it is added dropwise and finishes, then 2.2LS- (+)-epoxychloropropane (control temperature is no more than 15 DEG C) is slowly added dropwise, drop finishes 10 DEG C
Reaction 5 hours, reaction solution is slowly added into 18kg mixture of ice and water, and temperature is no more than 30 DEG C and is stirred to react 5 hours, and use is dense
Hydrochloric acid tune pH value is 1.0 or so, divides and removes organic layer, and water layer concentrated ammonia liquor tune pH is 7.0, stirring and crystallizing 24 hours, filters, uses
Water washing dries to obtain laevo-ornidazole 3.21kg.
Precision weighs sodium chloride 83g, and the laevo-ornidazole 50g of above method preparation adds water for injection 9000ml stirring to make
It is dissolved, and injects water to 10000ml.Add active carbon 10g to adsorb 15min, adjusts pH to 3.60 with dilute hydrochloric acid after filtering.
It is filling with 0.45 μm of filtering with microporous membrane, it is sealed after Zha Gai, then through 115 DEG C of steam sterilization 30min, obtain finished product, and repeat 5
It is secondary.
Embodiment 2
It is being protected from light, in the 50L reaction kettle under oxygen free condition, the 2- methyl-5-nitro miaow of 20L ethyl acetate and 2kg is added
Azoles is cooled to -10 DEG C, and the ethyl acetate solution (the about 1.1kg containing boron trifluoride) that boron trifluoride is slowly added dropwise (controls temperature not
More than 15 DEG C), it is added dropwise and finishes, then 2.2LS- (+)-epoxychloropropane (control temperature is no more than 15 DEG C) is slowly added dropwise, drop finishes 10 DEG C
Reaction 5 hours, reaction solution is slowly added into 18kg mixture of ice and water, and temperature is no more than 30 DEG C and is stirred to react 5 hours, and use is dense
Hydrochloric acid tune pH value is 1.0 or so, divides and removes organic layer, and water layer concentrated ammonia liquor tune pH is 7.0, stirring and crystallizing 24 hours, filters, uses
Water washing dries to obtain laevo-ornidazole 3.21kg.
Precision weighs sodium chloride 83g, and the laevo-ornidazole 50g of above method preparation, sodium nitrate 0.05g add water for injection
9000ml is stirred to dissolve, and injects water to 10000ml.Active carbon 10g is added to adsorb 15min, with dilute hydrochloric acid tune after filtering
Save pH to 3.60.It is filling with 0.45 μm of filtering with microporous membrane, it is sealed after Zha Gai, then through 115 DEG C of steam sterilization 30min, obtain
Finished product, and be repeated 5 times.
Embodiment 3
It is being protected from light, in the 50L reaction kettle under oxygen free condition, the 2- methyl-5-nitro miaow of 20L ethyl acetate and 2kg is added
Azoles is cooled to -10 DEG C, and the ethyl acetate solution (the about 1.1kg containing boron trifluoride) that boron trifluoride is slowly added dropwise (controls temperature not
More than 15 DEG C), it is added dropwise and finishes, then 2.2LS- (+)-epoxychloropropane (control temperature is no more than 15 DEG C) is slowly added dropwise, drop finishes 10 DEG C
Reaction 5 hours, reaction solution is slowly added into 18kg mixture of ice and water, and temperature is no more than 30 DEG C and is stirred to react 5 hours, and use is dense
Hydrochloric acid tune pH value is 1.0 or so, divides and removes organic layer, and water layer concentrated ammonia liquor tune pH is 7.0, stirring and crystallizing 24 hours, filters, uses
Water washing dries to obtain laevo-ornidazole 3.21kg.
Precision weighs sodium chloride 83g, and the laevo-ornidazole 50g of above method preparation, sodium nitrate 0.1g add water for injection
9000ml is stirred to dissolve, and injects water to 10000ml.Active carbon 10g is added to adsorb 15min, with dilute hydrochloric acid tune after filtering
Save pH to 3.60.It is filling with 0.45 μm of filtering with microporous membrane, it is sealed after Zha Gai, then through 115 DEG C of steam sterilization 30min, obtain
Finished product, and be repeated 5 times.
Embodiment 4
It is being protected from light, in the 50L reaction kettle under oxygen free condition, the 2- methyl-5-nitro miaow of 20L ethyl acetate and 2kg is added
Azoles is cooled to -10 DEG C, and the ethyl acetate solution (the about 1.1kg containing boron trifluoride) that boron trifluoride is slowly added dropwise (controls temperature not
More than 15 DEG C), it is added dropwise and finishes, then 2.2LS- (+)-epoxychloropropane (control temperature is no more than 15 DEG C) is slowly added dropwise, drop finishes 10 DEG C
Reaction 5 hours, reaction solution is slowly added into 18kg mixture of ice and water, and temperature is no more than 30 DEG C and is stirred to react 5 hours, and use is dense
Hydrochloric acid tune pH value is 1.0 or so, divides and removes organic layer, and water layer concentrated ammonia liquor tune pH is 7.0, stirring and crystallizing 24 hours, filters, uses
Water washing dries to obtain laevo-ornidazole 3.21kg.
45 DEG C, be protected from light, anaerobic protection under conditions of, precision weighs sodium chloride 83g, left-handed made from above-mentioned preparation method
Ornidazole 50g, sodium citrate 0.3g add water for injection 9000ml to stir to dissolve, inject water to 10000ml.Add
Active carbon 10g adsorbs 15min, adjusts pH to 3.60 with dilute hydrochloric acid after filtering.With 0.45 μm of filtering with microporous membrane, filling, Zha Gai
After seal, then through 115 DEG C of steam sterilization 30min, obtain finished product, and be repeated 5 times.
Embodiment 5
The content of nitrate anion in the L-ornidazole sodium chloride solution that comparative example 1-4 and embodiment 1-4 is obtained is detected respectively.
Nitrate anion in the L-ornidazole injection liquid is detected using liquid chromatography.Select Welch
Utimate XB-C18 (4.6mm × 250mm, 5 μm) chromatographic column.
Mobile phase: mobile phase is mixed by mobile phase A liquid with acetonitrile, and the two ratio is 80:20.Mobile phase A preparation side
Method are as follows: weigh the potassium dihydrogen phosphate of 1.36g in 1000ml ultrapure water, sufficiently dissolve, add four fourths of the 10% of 25ml
Base Ammonia is mixed, with 20% phosphoric acid tune pH value to 7.0, is filtered to obtain the final product.
Flow velocity: 0.8ml/min.
Column temperature: 30 DEG C.
Detection wavelength: 220nm.
Sample volume: 10 μ l.
Runing time: 30min.
When detection, using benchmark grade sodium nitrate as reference substance, nitrate anion in ornidazole sodium chloride injection is calculated with external standard method
Content.
Testing result is as shown in table 1.
The content of nitrate anion in table 1 comparative example 1-4 and embodiment 1-4
Group |
Nitrate anion (mg/l) |
Comparative example 1 |
32.1 |
Comparative example 2 |
31.3 |
Comparative example 3 |
35.8 |
Comparative example 4 |
56.2 |
Embodiment 1 |
5.0 |
Embodiment 2 |
10.2 |
Embodiment 3 |
14.9 |
Embodiment 4 |
1.4 |
As shown in Table 1, left-handed Austria prepared by the preparation method using heretofore described laevo-ornidazole
Nitre azoles, the L-ornidazole sodium chloride injection finished product (embodiment 1) of preparation, nitrate radical content is lower compared with the prior art, and
With statistical significance (p < 0.01).And the L-ornidazole sodium chloride injection of 4 preparation method of embodiment preparation is used,
Nitrate radical content is lower, also has statistical significance (p < 0.01) compared to the data of embodiment 1.
Embodiment 6
Long term toxicity test is carried out to L-ornidazole sodium chloride solution prepared by comparative example 1-4 and embodiment 1-4.
Healthy SD rat is taken, weight (135 ± 10) g, every group 30, half male and half female, experimental group presses the agent of daily 20g/kg
Amount administration, blank control group press same amount of normal saline stomach-filling.Observation one week, groups of animals activity, feed, excrement etc. before dispensing
Situation is without exception, then starts to be administered.
It is administered once, continuous 60 days, weighs weekly primary on time daily, adjust dosage according to changes of weight.Carry out appearance
The overviews such as sign, behavioral activity, fecal character, appetite, changes of weight.
After to medication, plucks eyeball and blood is taken to carry out blood picture, blood bio-chemistry checking, five coring, liver, spleen, lung, kidney internal organs claim
Weight, calculates the organ weight/power ratio of every 100g weight.
Experimental result is as shown in table 2.
The L-ornidazole injection liquid long term toxicity test result of table 2 comparative example 1-4 and embodiment 1-4 preparation
According to the experimental result of table 2 it is found that in L-ornidazole injection liquid, adverse reaction of the nitrate radical content to mouse
Incidence has significant impact, and the nitrate anion of high-content leads to the rising of adverse reaction rate.
Comparative example 1-4 group, the adverse reaction rate of rat are apparently higher than experimental example 1-4 group.Specifically, comparative example 1-4
In group, gastrointestinal system reaction such as diarrhea or constipation reaction are obviously higher than embodiment 1-4 group, and its data has statistics
Meaning (p < 0.01);Also have an impact in addition, high nitrate radical content reacts mood, the nervous system of mouse, such as 4 groups of comparative example
Data.Therefore the content for controlling nitrate anion becomes the one kind for reducing L-ornidazole sodium chloride injection adverse reaction rate
Feasible measure.
Embodiment 7
The finished product that comparative example 1-4 and embodiment 1-4 is obtained, is placed in 20 DEG C and is kept in dark place 18 months.
The content of nitrate anion in above-mentioned L-ornidazole sodium chloride solution is detected respectively.
Nitrate anion in the L-ornidazole injection liquid is detected using liquid chromatography.Select Welch
Utimate XB-C18 (4.6mm × 250mm, 5 μm) chromatographic column.
Mobile phase: mobile phase is mixed by mobile phase A liquid with acetonitrile, and the two ratio is 80:20.Mobile phase A preparation side
Method are as follows: weigh the potassium dihydrogen phosphate of 1.36g in 1000ml ultrapure water, sufficiently dissolve, add four fourths of the 10% of 25ml
Base Ammonia is mixed, with 20% phosphoric acid tune pH value to 7.0, is filtered to obtain the final product.
Flow velocity: 0.8ml/min.
Column temperature: 30 DEG C.
Detection wavelength: 220nm.
Sample volume: 10 μ l.
Runing time: 30min.
When detection, using benchmark grade sodium nitrate as reference substance, nitrate anion in ornidazole sodium chloride injection is calculated with external standard method
Content.
Testing result is as shown in table 3.
320 DEG C of table be kept in dark place 18 months after in comparative example 1-4 and embodiment 1-4 nitrate anion content
As shown in Table 3, left-handed Austria prepared by the preparation method using heretofore described laevo-ornidazole
Nitre azoles, the L-ornidazole sodium chloride injection finished product of preparation, nitrate radical content is lower after being kept in dark place at 20 DEG C 18 months;And
During preservation, the nitrate anion of generation is less than the product that the prior art produces for it, and data have statistical significance (p <
0.01)。
Embodiment 8
The finished product that comparative example 1-4 and embodiment 1-4 is obtained, is placed in 20 DEG C and is kept in dark place 18 months.To above-mentioned finished product by real
The method for applying example 6 carries out long term toxicity test.
Experimental result is as shown in table 4.
The L-ornidazole injection liquid long term toxicity test result of table 4 comparative example 1-4 and embodiment 1-4 preparation
According to the experimental result of table 4 it is found that in L-ornidazole injection liquid, adverse reaction of the nitrate radical content to mouse
Incidence has significant impact, and the nitrate anion of high-content leads to the rising of adverse reaction rate.
Comparative example 1-4 group, the adverse reaction rate of rat are apparently higher than experimental example 1-4 group.Specifically, comparative example 1-4
In group, nervous system reaction such as spasm or reaction of trembling are obviously higher than embodiment 1-4 group, and its data is anticipated with statistics
Adopted (p < 0.01);In comparative example 1-4 group, gastrointestinal system reaction such as diarrhea or constipation reaction are obviously higher than embodiment 1-4
Group, and its data has statistical significance (p < 0.01);In comparative example 1-4 group, the abnormal feeling of mouse, such as abnormal sounding
Or abnormal aggression implementations, also it is higher than embodiment 1-4 group, and its data has statistical significance (p < 0.01).Further
The importance of nitrate radical content in control L-ornidazole injection liquid is demonstrated, and demonstrates left-handed Austria prepared by the present invention
The stability and safety of nitre azoles injection.
It should be pointed out that for those of ordinary skill in the art, without departing from the principle of the present invention, may be used also
With several improvements and modifications are made to the present invention, these improvements and modifications also fall within the scope of protection of the claims of the present invention.