CN107736381A - A kind of microorganism for being used to prevent and treat sucking insect and chemical insecticide compounded combination - Google Patents
A kind of microorganism for being used to prevent and treat sucking insect and chemical insecticide compounded combination Download PDFInfo
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- CN107736381A CN107736381A CN201711200746.7A CN201711200746A CN107736381A CN 107736381 A CN107736381 A CN 107736381A CN 201711200746 A CN201711200746 A CN 201711200746A CN 107736381 A CN107736381 A CN 107736381A
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- Prior art keywords
- ethyl ester
- spiral shell
- serratia marcescens
- shell worm
- worm ethyl
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having no bond to a nitrogen atom
- A01N47/06—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having no bond to a nitrogen atom containing —O—CO—O— groups; Thio analogues thereof
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of microorganism for being used to prevent and treat sucking insect and chemical insecticide compounded combination, its active component is serratia marcescens and spiral shell worm ethyl ester, and content of the serratia marcescens in the insecticides is 108~1011Cfu/ml, percentage by weight of the spiral shell worm ethyl ester in the insecticides are 10%~45%.There is obvious synergistic effect to sucking insects such as preventing and treating planthopper, aphids after two groups of active components compounding of the present invention, it can reduce chemical pesticide dosage, improve microorganism to insect lethal speed, extend the duration of desinsection, the advantages that delaying drug resistance, there is good application and popularization value.
Description
First, technical field
The invention belongs to field of pest control, and in particular to it is a kind of be used for prevent and treat sucking insect serratia marcescens and
Spiral shell worm ethyl ester compounded combination.
2nd, background technology
Spiral shell worm ethyl ester (spirotetramat) is tetronic acid class compound, is a brand-new insecticide, is passed with most of
Insecticide of uniting is different, the medicament can in whole plant two-way Uptake and translocation, it is long, right to the sucking pest prevention and control phase to have
Natural enemy is safer, with plant compatibility it is good the advantages that.In Field information, spiral shell worm ethyl ester shows good photostability
And residual activity, and cross resistance is not present in most chemical insecticides with applying at present.But long-term use also can not
What is avoided causes the generation of pest resistance to insecticide.
Serratia marcescens (Serratia marcescens) is that a kind of Insect Pathogenic being widely present in nature is thin
Bacterium, the various pests such as Diptera, Homoptera, Semiptera can be infected.Serratia marcescens S-JS1 bacterial strains are from susceptible brown paddy plant hopper worm
A kind of new pathogenic strain that separation obtains in vivo, the bacterial strain has preferable insecticidal activity to brown paddy plant hopper, to small brown rice planthopper, aphid
Also there is certain preventive effect Deng sucking insect.
Serratia marcescens is mixed with chemical agent progress science, the dosage of chemical pesticide can be not only reduced, is improved
The quick-acting of microbial insecticide, and with the generation for delaying pest resistance to insecticide, reduce chemical pesticide residual of agricultural product etc.
Advantage.Therefore, the safety of serratia marcescens of the present invention and spiral shell worm ethyl ester compounded combination for current sucking insect
Efficiently prevent and treat significant.
3rd, the content of the invention
It is an object of the invention to the deficiency for existing alone chemical insecticide or biological insecticides technology, there is provided a kind of
The biology of effectively control sucking insect combines with pest control with insecticide, after serratia marcescens and spiral shell worm ethyl ester compounding, to a variety of
Sucking insect has obvious synergistic function.
4th, technical scheme
To reach above-mentioned purpose, the present invention adopts the following technical scheme that：
A kind of Pesticidal combination, its active component include serratia marcescens and spiral shell worm ethyl ester, wherein, serratia marcescens
Content in Pesticidal combination is 108~1011Cfu/ml, percentage by weight of the spiral shell worm ethyl ester in the Pesticidal combination are
10%~45%.
The culture of serratia marcescens：
Culture medium：LB (tryptone 10g, sodium chloride 10g, yeast extract 5g, agar 20g, deionized water 1L).
Condition of culture：At 28~37 DEG C, 2~5d is cultivated.
The Pesticidal combination of the present invention in addition to the active ingredient (s, can be aided with surface-active conventional in Pesticide formulation
Agent, auxiliary agent, filler and/or carrier are simultaneously prepared into any one formulation for being adapted to agriculturally use according to the actual requirements, including but
It is not limited to suspending agent, aqueous emulsion, microcapsule suspending agent, wettable powder, water dispersible granules, microemulsion etc..To be selected in specifically used
Selecting the type of dosage form of processing can actually determine to obtain according to using the different of object, use environment etc..
It is compared with the prior art, the advantages of the present invention are：1. the Pesticidal combination of the present invention and chemistry
Insecticide is alone to be compared, and is had obvious synergy and is extended lasting period effect；2. the usage amount of agricultural chemicals is reduced, environmental pollution subtracts
It is few；3. use range is wider, there is well simultaneous to control effect simultaneous various pests.
5th, embodiment
With reference to embodiment to further instruction of the present invention, the percentage in embodiment is weight percentage, but
The invention is not limited in this.
Embodiment 1：1011Insecticidal effect of the spiral shell worm ethyl ester suspending agent of cfu/ml serratia marcescens 24% to planthopper
Serratia marcescens 1011Cfu/ml, spiral shell worm ethyl ester 24%, aliphatic amine polyoxyethylene are phonetic 2.5%, sldium lauryl sulfate
4%th, silicone 0.2%, sodium alginate 0.1%, glycerine 0.05%, deionized water add to 100%, are made 1011Cfu/ml glues
The spiral shell worm ethyl ester suspending agent of matter Serratieae 24%.
For trying object：Planthopper
For examination microorganism and medicament：1011Cfu/ml serratia marcescens suspending agents, 24% spiral shell worm ethyl ester suspending agent, are pressed
Obtained according to the above method.
Insecticidal effect of the Pesticidal combination of table one to planthopper
Test method：Each reagent agent is accurately weighed respectively with balance, and related agents are prepared by every mu of 50L spray amount,
Even spraying, the planthopper on rice is prevented and treated, using clear water as blank control (CK), 3 repetitions are each handled, respectively at 1d, 3d
With dead borer population, borer population living are counted after 5d, calculate average decline rate and Rectifying efficacies.
Calculation formula is as follows：
As a result 1d, 3d and 5d prevention effect are respectively after showing serratia marcescens spiral shell worm ethyl ester suspending agent medicine of the present invention
58.62%th, 78.95% and 89.09%, and 1011Cfu/m serratia marcescens suspending agent and 24% spiral shell worm ethyl ester suspending agent according to
Same method uses, 1d, 3d and 5d prevention effect are respectively 9.70%, 21.43%, 30.46% and 27.59%, 47.37%,
61.82%, illustrate that the present invention is substantially better than single dose serratia marcescens suspending agent to the preventive effect of planthopper and spiral shell worm ethyl ester suspends
Agent, as a result as shown in Table 1.
Embodiment 2：1010Insecticidal effect of the spiral shell worm ethyl ester suspending agent of cfu/ml serratia marcescens 35% to planthopper
Serratia marcescens 1010Cfu/ml, spiral shell worm ethyl ester 35%, naphthalene sulfonic acid-formaldehyde condensation product 1.5%, detergent alkylate sulphur
Sour sodium 3.5%, C10-20 saturated fat acids 0.2%, gelatin 0.1%, ethylene glycol 1%, deionized water add to 100%,
1010The spiral shell worm ethyl ester suspending agent of cfu/ml serratia marcescens 35%.
Test method is with dosage with embodiment 2, the results showed that the present embodiment serratia marcescens spiral shell worm ethyl ester suspending agent
1d, 3d and 5d prevention effect are respectively 60.83%, 81.04% and 92.81% after medicine, and 1010Cfu/ml serratia marcescens hangs
Floating agent and 35% spiral shell worm ethyl ester suspending agent use according to same method, after medicine 1d, 3d and 5d prevention effect be respectively 6.89%,
17.54%th, 27.27% and 36.21%, 52.63%, 67.27%, illustrate that the present invention is substantially better than single dose to the preventive effect of planthopper
Serratia marcescens suspending agent and spiral shell worm ethyl ester suspending agent.
Embodiment 3：108The spiral shell worm ethyl ester microcapsule suspending agent of cfu/ml serratia marcescens 45% is imitated to the desinsection of planthopper
Fruit
Serratia 108Cfu/ml, spiral shell worm ethyl ester 45%, naphthalene sulfonic acid-formaldehyde condensation product 5%, wetting and penetrating agent F 4%,
PIC 6%, sodium tripolyphosphate 0.05%, deionized water add to 100%, are made 108Cfu/ml Serratias
45% spiral shell worm ethyl ester microcapsule suspending agent.
Test method is with dosage with embodiment 2, the results showed that the present embodiment serratia marcescens spiral shell worm ethyl ester micro-capsule hangs
1d, 3d and 5d prevention effect are respectively 62.07%, 85.96% and 94.55% after floating agent medicine, and 108Cfu/ml Serratias
Bacterium suspending agent and 45% spiral shell worm ethyl ester microcapsule suspending agent use according to same method, and 1d, 3d and 5d prevention effect are respectively after medicine
3.45%th, 8.77%, 12.73% and 41.38%, 56.14%, 72.73%, illustrate that the present invention is substantially excellent to the preventive effect of planthopper
In single dose serratia marcescens microcapsule suspending agent and spiral shell worm ethyl ester microcapsule suspending agent.
Embodiment 4：109Insecticidal effect of the spiral shell worm ethyl ester aqueous emulsion of cfu/ml serratia marcescens 25% to melon aphid
Serratia 109Cfu/ml, spiral shell worm ethyl ester 25%, cyclohexanone 6%, phenyl phenol polyoxyethyl ether 2.5%, the third three
Alcohol 0.5%, xanthans 0.05%, C10-20 saturated fat acids 0.1%, deionized water add to 100%, are made 109cfu/ml
The spiral shell worm ethyl ester aqueous emulsion of serratia marcescens 25%.
For trying object：Aphid, melon aphid
For examination microorganism and medicament：Serratia marcescens 109Cfu/ml, 25% spiral shell worm ethyl ester aqueous emulsion, according to above-mentioned
Method obtains.
Test method：Spray-on process.Each reagent agent is accurately weighed respectively with balance, by every mu of 50L spray amount, uniformly spray
Mist, the aphid on cucumber is prevented and treated, using clear water as blank control (CK), each handle 3 repetitions, count dead after 1d and 2d
Borer population, borer population living are died, calculates average decline rate and Rectifying efficacies.
As a result 1d and 2d prevention effects are distinguished after showing the present embodiment serratia marcescens spiral shell worm ethyl ester aqueous emulsion agent medicine
For 90.91% and 98.11%, and serratia marcescens 109Cfu/ml and 25% spiral shell worm ethyl ester aqueous emulsion make according to same method
With 1d and 2d prevention effects are respectively 3.64%, 5.66% and 61.82%, 71.69% after medicine, illustrate the present invention to aphid
Preventive effect is substantially better than single dose serratia marcescens aqueous emulsion and spiral shell worm ethyl ester aqueous emulsion.As a result as shown in Table 2.
Table 2 109Insecticidal effect of the spiral shell worm ethyl ester aqueous emulsion of cfu/ml serratia marcescens 25% to aphid
Embodiment 5：1010Insecticidal effect of the spiral shell worm ethyl ester aqueous emulsion of cfu/ml serratia marcescens 15% to melon aphid
Serratia 1010Cfu/ml, spiral shell worm ethyl ester 15%, 1-METHYLPYRROLIDONE 18%, NPE
Phosphate 8%, glycerine fatty acid APEO 8%, Arabic gum 5%, phosphoric acid isobutyl ester 1%, ethylene glycol 4%, water is supplied
To 100%, 10 are made10The spiral shell worm ethyl ester aqueous emulsion of cfu/ml serratia marcescens 15%.
Test method is the same as embodiment 4, the results showed that 1d after the present embodiment serratia marcescens spiral shell worm ethyl ester aqueous emulsion medicine
It is respectively 87.27% and 92.45% with 2d prevention effects, and serratia marcescens 1010Cfu/ml and 15% spiral shell worm ethyl ester water and milk
Agent uses according to same method, 1d and 2d prevention effects are respectively 10.91%, 18.87% and 43.64%, 60.38% after medicine,
Illustrate that the present invention is substantially better than single dose serratia marcescens aqueous emulsion and spiral shell worm ethyl ester aqueous emulsion to the preventive effect of aphid.As a result such as table
Shown in three.
Table 3 1010Insecticidal effect of the spiral shell worm ethyl ester aqueous emulsion of cfu/ml serratia marcescens 15% to aphid
Claims (2)
1. a kind of microorganism and chemical insecticide compounded combination, it is characterised in that compounded combination is serratia marcescens and spiral shell worm
The content of ethyl ester, wherein serratia marcescens in compounded combination is 108~1011Cfu/ml, spiral shell worm ethyl ester is in compounded combination
Percentage by weight be 10%~45%.
2. compounded combination according to claim 1, it is characterised in that compounded combination is used to prevent and treat the thorn such as planthopper, aphid
Absorption insect.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711200746.7A CN107736381A (en) | 2017-11-24 | 2017-11-24 | A kind of microorganism for being used to prevent and treat sucking insect and chemical insecticide compounded combination |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711200746.7A CN107736381A (en) | 2017-11-24 | 2017-11-24 | A kind of microorganism for being used to prevent and treat sucking insect and chemical insecticide compounded combination |
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| Publication Number | Publication Date |
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| CN107736381A true CN107736381A (en) | 2018-02-27 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711200746.7A Pending CN107736381A (en) | 2017-11-24 | 2017-11-24 | A kind of microorganism for being used to prevent and treat sucking insect and chemical insecticide compounded combination |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111357770A (en) * | 2020-05-12 | 2020-07-03 | 广东省生物资源应用研究所 | Application of serratia marcescens in preparation of termite antifeedant |
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| CN106818798A (en) * | 2016-12-15 | 2017-06-13 | 天津联合益农科技有限公司 | A kind of compounded pesticides containing anabasine compound |
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Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103875700A (en) * | 2012-12-21 | 2014-06-25 | 陕西标正作物科学有限公司 | Insecticidal composition and applications thereof in controlling insects with piercing-sucking mouthpart |
| CN103478151A (en) * | 2013-09-17 | 2014-01-01 | 江苏省绿盾植保农药实验有限公司 | Pesticide composition containing spirotetramat |
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| CN105794827A (en) * | 2016-05-26 | 2016-07-27 | 深圳诺普信农化股份有限公司 | Insecticidal composition containing spirotetramat |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111357770A (en) * | 2020-05-12 | 2020-07-03 | 广东省生物资源应用研究所 | Application of serratia marcescens in preparation of termite antifeedant |
| CN111357770B (en) * | 2020-05-12 | 2020-08-28 | 广东省生物资源应用研究所 | Application of serratia marcescens in preparation of termite antifeedant |
| WO2021057308A1 (en) * | 2020-05-12 | 2021-04-01 | 广东省科学院动物研究所 | Use of serratia marcescens in preparing termite antifeedant |
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