CN107714733B - Preparation method of antibacterial peptide gynecological lotion - Google Patents

Preparation method of antibacterial peptide gynecological lotion Download PDF

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CN107714733B
CN107714733B CN201711212780.6A CN201711212780A CN107714733B CN 107714733 B CN107714733 B CN 107714733B CN 201711212780 A CN201711212780 A CN 201711212780A CN 107714733 B CN107714733 B CN 107714733B
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bee
antibacterial peptide
solution
glycerol
bees
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CN107714733A (en
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尹志明
刘动勤
彭松涛
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Jian Royal Beauty Health Co Ltd
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56Materials from animals other than mammals
    • A61K35/63Arthropods
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • A61K38/02Peptides of undefined number of amino acids; Derivatives thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/02Inorganic compounds
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/22Heterocyclic compounds, e.g. ascorbic acid, tocopherol or pyrrolidones
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/46Ingredients of undetermined constitution or reaction products thereof, e.g. skin, bone, milk, cotton fibre, eggshell, oxgall or plant extracts
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00Medicinal preparations characterised by special physical form
    • A61K9/0012Galenical forms characterised by the site of application
    • A61K9/0034Urogenital system, e.g. vagina, uterus, cervix, penis, scrotum, urethra, bladder; Personal lubricants

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
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  • Gynecology & Obstetrics (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
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Abstract

The invention discloses a preparation method of antibacterial peptide gynecological lotion, which comprises the following steps: 1) culturing bee in an environment containing Bacillus caldarius and Bacillus pseudolaricii, heating to dry, adding Glycyrrhrizae radix tablet, and pulverizing to obtain bee powder; 2) soaking bee powder in glycerol-acetic acid solution containing glucose, and ultrasonic vibrating to obtain mixed solution; 3) filtering the mixed solution to remove filter residue, and freeze-drying the filtrate to obtain bee extract; 4) adding bee extract and vitamin C into cucumber juice, stirring, adding MgSO4 solution, and stirring. The antibacterial peptide gynecological lotion prepared by the method can be absorbed by patients gently, and can create healthy and comfortable breeding and living environment for beneficial flora while killing pathogenic flora.

Description

Preparation method of antibacterial peptide gynecological lotion
Technical Field
The invention relates to the field of gynecology, in particular to a preparation method of antibacterial peptide gynecological lotion.
Background
Under the action of endocrine hormone in vagina of healthy women, epithelial cells proliferate, and surface cells contain abundant glycogen, which is beneficial to the growth of lactobacillus, and the bacterium accounts for more than 90% of the vagina. The lactobacillus existing in large quantity inhibits the growth of other pathogenic bacteria, and forms a normal ecological balance in the vagina.
When external pathogens invade a large amount of pathogens to destroy the defense function of the vagina, the microbial population normally parasitizing in the vagina is unbalanced in microecological balance, beneficial bacteria such as lactobacillus in the vagina are reduced, other bacteria are propagated in a large amount, the anaerobic bacteria are propagated to simultaneously generate amine substances, the vagina is alkalized, vaginal secretion is increased and odor is generated, and bacterial vaginitis is finally caused.
The traditional product for treating vaginitis mainly treats sterilization, antibiotics with an antibacterial effect or alkaline liquid is used for washing vagina, the antibiotics are used for killing pathogenic microorganisms and killing a leading flora (lactobacillus and bifidobacterium vaginalis), although symptoms are relieved during administration, other mixed bacteria are rapidly bred once the medicine is stopped, and inflammation is caused to occur repeatedly and alternately. The long-term use of antibiotics and antibacterial drugs can also cause drug resistance of various pathogens and aggravate the flora imbalance in the vagina. The composition of the used lotion is completely different from that of vaginal secretion, repeated vaginal douche can stimulate the vagina, destroy the vaginal secretion environment, accelerate the vaginal aging and easily cause the vagina to be dry and astringent.
CN101099815A discloses a fragrant grass vagina cleaning lotion and a preparation process thereof, and particularly discloses a vagina cleaning lotion formed by mixing a plurality of Chinese herbal medicines, royal jelly, vitamin C and other raw materials, wherein dozens of Chinese herbal medicines and royal jelly have high cost and long preparation time, only respectively play a role and lack of synergistic effect. CN105079405A discloses miconazole nitrate compound lotion and a preparation method thereof, and particularly discloses a vaginal lotion formed by mixing extracted and concentrated traditional Chinese medicine components with miconazole nitrate, wherein miconazole nitrate can inhibit the growth of fungi and lactobacillus, and the vaginal lotion is large in stimulation during use and easy to damage vaginal tissues.
If the gynecological lotion which is easy to absorb smoothly and is beneficial to the flora to create healthy and comfortable breeding and living environment while killing pathogenic flora can be developed, the gynecological lotion is a great gospel for the majority of female patients suffering from vaginitis.
Disclosure of Invention
The technical problem to be solved by the invention is to overcome the defects of the prior art and provide a preparation method of gynecological lotion, which can be absorbed by patients gently, and creates healthy and comfortable breeding and living environment for beneficial flora while killing pathogenic flora.
The technical solution of the invention is as follows: a preparation method of antibacterial peptide gynecological lotion comprises the following steps:
1) placing bees in an environment containing gardnerella and bacteroides for 1-2 h, heating at 50-70 deg.C for 1-2 h, oven drying the whole bees, adding sliced radix Glycyrrhizae, and pulverizing to obtain bee powder; the mass ratio of the liquorice tablets to the bees is 1: 8-1: 10;
2) putting the bee powder into a glycerol-acetic acid solution containing glucose, soaking for 30-40 h, and then ultrasonically vibrating for 10-20 min to obtain a mixed solution;
3) filtering the mixed solution to remove filter residue, and freeze-drying the filtrate to obtain bee extract;
4) adding the bee extract and vitamin C into cucumber juice, stirring at 30-50 ℃ for 20-40 min, and adding 0.6-1.2 mmol/L MgSO4Stirring the solution to obtain the product.
Preferably, the bee is any one of Apis mellifera, Apis anatoria, Apis gaboxensis, Apis caribbeana, and Apis karebauqian.
Preferably, the particle size of the bee powder is 1-5 mm.
Preferably, the volume fraction of glycerol in the glycerol-acetic acid solution in the step 2) is 15-30%.
Preferably, the concentration of the glucose in the glycerol-acetic acid solution in the step 2) is 0.5-1 mol/L
Preferably, the step 3) of freeze-drying refers to freezing at-10 to-30 ℃, and then dehydrating and drying under the vacuum condition of 2-10 Pa.
Preferably, the mass concentration of the cucumber juice in the step 4) is 5-10%.
Preferably, the components in the step 4) are prepared according to the following proportions in parts by mass: 4-8 parts of bee extract, 30-50 parts of cucumber juice, 1-3 parts of vitamin C and MgSO4And 3-5 parts of a solution.
Bee venom is secreted from the venom gland of bees and contains active enzymes, polypeptides, carbohydrates, lipids, amino acids, amines and other active compounds, so that the bee venom has various pharmacological properties and biological activities such as cancer resistance, radiation resistance, antibiosis, antiphlogosis, mutation resistance and the like, but the specific extraction method and the application of the bee venom are not researched in detail. Because the bees can spontaneously secrete corresponding antibacterial peptides under the condition of a large amount of viruses and bacteria, and the immunity of the bees is superior to that of the delicate vagina of a human body, more secreted antibacterial peptides and better antibacterial effect are achieved. Through the steps 1), 2) and 3), crushing, soaking, extracting and filtering, the obtained bee extract contains a large amount of antibacterial peptides with excellent antibacterial effect, can efficiently kill harmful bacteria and promote the growth of lactobacillus.
The invention has the beneficial effects that:
1) the gynecological lotion is prepared by extracting and processing bees, wherein the bees are cultured in an environment which is similar to vaginitis and contains gardnerella and bacteroides, and the bees are stimulated to secrete a large amount of antibacterial peptides for resisting the gardnerella and the bacteroides, so that the product is easy to be absorbed by the vagina gently, a healthy and mild secretion environment is created, and a healthy and comfortable breeding and living environment is created by beneficial floras while pathogenic floras are killed;
2) the liquorice tablets and the dried bees are crushed, so that antibacterial peptide ingredients in the bees are favorably emitted from hard shells such as wings, feet, breasts and the like, the effective ingredients and the yield of the bee extract are improved, and the sterilizing and inflammation diminishing effects are improved;
3) soaking bee powder in glycerol-acetic acid solution containing glucose to extract antibacterial peptide from bee powder to the maximum extent, and keeping the effect of antibacterial peptide, wherein the glycerol-acetic acid buffer solution is matched to increase solubility and absorption rate;
4) filtering to remove solid impurities, wherein the material composition is liquid phase, and freeze-drying to maintain the antibacterial quality of the antibacterial peptide in the bee body and further increase the solubility of the bee extract;
5) vitamin C, MgSO4The expression of the antibacterial peptide in the bee extract is promoted, the antibacterial peptide is dissolved in cucumber juice, so that the full absorption and maintenance of the antibacterial peptide in the vagina are facilitated, the wettability is increased, and excellent propagation conditions are provided for lactobacillus;
6) the antibacterial peptide gynecological lotion prepared by the components through synergistic action and multi-step processing can kill pathogenic flora and simultaneously create healthy and comfortable breeding and living environment for beneficial flora, thereby effectively relieving vaginitis.
Detailed Description
The present invention will be described in further detail with reference to the following examples, but the present invention is not limited to the following examples.
The following examples are not provided to limit the scope of the present invention, nor are the steps described to limit the order of execution. Modifications of the invention which are obvious to those skilled in the art in view of the prior art are also within the scope of the invention as claimed.
Example one
A preparation method of antibacterial peptide gynecological lotion comprises the following steps:
1) placing bees in an environment containing gardnerella and Bacteroides for 1-2 h, heating at 50 deg.C for 2h, oven drying the whole bees, adding Glycyrrhrizae radix tablet, and pulverizing to obtain bee powder; the mass ratio of the liquorice tablets to the bees is 1: 8; the bee is an Italian bee; the particle size of the bee powder is 1 mm;
2) soaking bee powder in glycerol-acetic acid solution containing glucose for 30 hr, and ultrasonically vibrating for 10min to obtain mixed solution; the volume fraction of the glycerol in the glycerol-acetic acid solution is 15 percent; the concentration of the glucose in the glycerol-acetic acid solution is 0.5 mol/L;
3) filtering the mixed solution to remove filter residue, freezing the filtrate at-10 deg.C, and dehydrating and drying under 2Pa vacuum condition to obtain bee extract;
4) the composition is prepared from the following components in parts by mass: 4 parts of bee extract, 30 parts of cucumber juice with the mass concentration of 5%, 1 part of vitamin C and MgSO43 parts of a solution; adding bee extract and vitamin C into cucumber juice, stirring at 30 deg.C for 40min, adding 0.6mmol/L MgSO4Stirring the solution to obtain the product.
Example two
A preparation method of antibacterial peptide gynecological lotion comprises the following steps:
1) placing bees in an environment containing gardnerella and Bacteroides for 1-2 h, heating at 70 deg.C for 1h, oven drying the whole bees, adding Glycyrrhrizae radix tablet, and pulverizing to obtain bee powder; the mass ratio of the liquorice tablets to the bees is 1: 10; the bee is a bee with royal jelly high yield; the particle size of the bee powder is 5 mm;
2) soaking bee powder in glycerol-acetic acid solution containing glucose for 40 hr, and ultrasonically vibrating for 20min to obtain mixed solution; the volume fraction of glycerol in the glycerol-acetic acid solution is 30 percent; the concentration of the glucose in the glycerol-acetic acid solution is 1 mol/L;
3) filtering the mixed solution to remove filter residue, freezing the filtrate at-30 deg.C, and dehydrating and drying under 10Pa vacuum condition to obtain bee extract;
4) the composition is prepared from the following components in parts by mass: 8 parts of bee extract, 50 parts of cucumber juice with the mass concentration of 10%, 3 parts of vitamin C and MgSO45 parts of a solution; adding bee extract and vitamin C into cucumber juice, stirring at 50 deg.C for 20min, adding 1.2mmol/L MgSO4Stirring the solution to obtain the product.
EXAMPLE III
A preparation method of antibacterial peptide gynecological lotion comprises the following steps:
1) placing bees in an environment containing gardnerella and Bacteroides for 1-2 h, heating at 60 deg.C for 1.5h, oven drying the whole bees, adding Glycyrrhrizae radix tablet, and pulverizing to obtain bee powder; the mass ratio of the liquorice tablets to the bees is 1: 9; the bee is an anatolya bee; the particle size of the bee powder is 1-5 mm;
2) soaking bee powder in glycerol-acetic acid solution containing glucose for 35 hr, and ultrasonically vibrating for 15min to obtain mixed solution; the volume fraction of the glycerol in the glycerol-acetic acid solution is 20 percent; the concentration of the glucose in the glycerol-acetic acid solution is 0.8 mol/L;
3) filtering the mixed solution to remove filter residue, freezing the filtrate at-20 deg.C, and dehydrating and drying under 5Pa vacuum condition to obtain bee extract;
4) the composition is prepared from the following components in parts by mass: 6 parts of bee extract, 40 parts of cucumber juice with the mass concentration of 8%, 2 parts of vitamin C and MgSO44 parts of a solution; adding bee extract and vitamin C into cucumber juice, stirring at 40 deg.C for 30min, adding 0.9mmol/L MgSO4Stirring the solution to obtain the product.
Product testing
Firstly, testing bacteriostatic effect
1. Culturing test bacteria: activating pathogenic flora (Bacillus caldarius and Bacteroides) and beneficial flora (Lactobacillus, Bifidobacterium vaginalis), selecting single colony to 5ml corresponding liquid culture medium, and culturing at 37 deg.C for 12 hr; and (3) diluting 100 mu L of the bacterial suspension by 10 times, putting 100 mu L of the bacterial suspension on a solid culture medium plate, and uniformly coating.
2. Preparing different gel samples A, B, C, wherein A is the antibacterial peptide gynecological lotion of the invention, and the preparation is carried out according to the instruction; b is the existing gynecological lotion (refer to CN105079405A), the lotion comprises miconazole nitrate, rhizoma corydalis, fructus Cucumidis Sativi, radix Sophorae Flavescentis, Catechu, herba Lobeliae chinensis, herba Euphorbiae Humifusae, cortex Dictamni Radicis, Polygoni Multiflori radix, and folium Artemisiae Argyi, and the preparation method comprises preparing materials, extracting effective components of Chinese medicinal materials, and preparing lotion; c is the existing bee antibacterial peptide (refer to CN106146608A), the extraction of the bee antibacterial peptide from the fermentation liquor mainly comprises the following steps: 1) sterilizing and filtering the fermentation liquor, filtering the fermentation filtrate by using an ultrafiltration membrane, and removing soluble impurities with large molecular weight in the filtrate; 2) concentrating the ultrafiltered filtrate by using a nanofiltration membrane to remove impurities such as water, inorganic salt and the like; 3) separating the filtered filtrate by using a molecular sieve column Sephacryl; 4) spray drying the concentrated solution to obtain the bee antibacterial peptide product.
3. And (3) beating the filter paper into a circular sheet with the radius of 3mm by using a perforating machine, sterilizing for 30min by using high-pressure steam at 120 ℃, and drying to obtain the sterile filter paper sheet.
4. Different gel samples A, B, C were applied to the surface of the aseptic filter paper sheet, the thickness of the applied gel samples was 10 μm, the applied surface was attached to a bacteria-containing plate, the plate was cultured at 37 ℃ for 12 hours, and the radius of the zone of inhibition of the filter paper sheet was measured, the results are shown in Table 1.
TABLE 1 radius of inhibition zone (mm) of different lotions for each bacterium
Gel sample Gardnerella sp Bacteroides Lactobacillus strain Vaginal bifidobacteria
A 8.3 7.2 2.9 2.6
B 5.1 4.9 5.1 4.7
C 3.4 3.5 3.1 3.4
The antibacterial effect is shown by the inhibition zone, namely the larger the radius of the inhibition zone is, the better the antibacterial effect is. The test uses different bacteriostatic substances, each bacteriostatic substance has the same dosage, and the bacteriostatic substances are smeared on the same sterile filter paper. As can be seen from Table 1, A has excellent antibacterial effect on pathogenic flora (Gardnerella, Bacteroides) and does not cause strong damage to beneficial flora (Lactobacillus, Bifidobacterium vaginalis); b has strong antibacterial effect on pathogenic flora (Bacillus clarkii and Bacteroides) and beneficial flora (Lactobacillus, Bifidobacterium vaginalis); c can kill pathogenic bacteria (Bacillus caldarius and Bacteroides) in small amount, and does not affect the growth of beneficial bacteria (Lactobacillus and Bifidobacterium vaginalis). In combination with the comparison, it is obvious that the antibacterial peptide gynecological lotion can efficiently kill pathogenic flora (gardnerella and anaerobes) and create healthy and mild growth and reproduction conditions for beneficial flora lactobacillus, so as to adjust the imbalance vaginal flora balance in the vaginitis environment.
Secondly, testing the synergistic effect of the components
1. Culturing test bacteria: activating pathogenic flora (Bacillus caldarius and Bacteroides) and beneficial flora (Lactobacillus, Bifidobacterium vaginalis), selecting single colony to 5ml corresponding liquid culture medium, and culturing at 37 deg.C for 12 hr; and (3) diluting 100 mu L of the bacterial suspension by 10 times, putting 100 mu L of the bacterial suspension on a solid culture medium plate, and uniformly coating.
2. Preparing different lotion samples A, A1, A2 and A3, wherein A is the antibacterial peptide gynecological lotion prepared according to the specification; a1 is gel prepared by removing cucumber juice in step 4) of the invention; a2 is the lotion prepared by eliminating vitamin C in the step 4) of the invention; a3 is MgSO eliminated in step 4) of the invention4The lotion is prepared from the solution.
3. And (3) beating the filter paper into a circular sheet with the radius of 3mm by using a perforating machine, sterilizing for 30min by using high-pressure steam at 120 ℃, and drying to obtain the sterile filter paper sheet.
4. Different gel samples A, A1, A2 and A3 are smeared on the surface of the sterile filter paper sheet, the smearing thickness is 10 mu m, the smearing surface is pasted on a bacterium-containing flat plate, the culture is carried out for 12h at 37 ℃, the radius of the inhibition zone of the filter paper sheet is measured, and the specific result is shown in Table 2.
TABLE 2 radius of zone of inhibition (mm) for different component gel samples for each bacterium
Gel sample Gardnerella sp Bacteroides Lactobacillus strain Vaginal bifidobacteria
A 8.3 7.2 2.9 2.6
A1 7.8 6.1 4.2 3.7
A2 7.4 6.5 3.7 3.5
A3 7.2 6.3 3.5 3.8
As can be seen from Table 2, A1, A2 and A3 are the test results after part of the components are removed, the radius of inhibition zones of Gardnerella and Bacteroides is smaller than that of A, which indicates that cucumber juice, vitamin C or MgSO (MgSO) are removed4The antibacterial effect of the solution on pathogenic flora is reduced; the radius of lactobacillus and vaginal bifidobacterium is larger than that of A, which indicates that cucumber juice, vitamin C or MgSO is not used4The solution is not favorable for the propagation of beneficial flora. The invention A is adopted to mix the bee extract with cucumber juice, vitamin C and MgSO4The solutions are mixed to prepare the gel with the best antibacterial effect.
Third, method unicity test
1. Culturing test bacteria: activating pathogenic flora (Bacillus caldarius and Bacteroides) and beneficial flora (Lactobacillus, Bifidobacterium vaginalis), selecting single colony to 5ml corresponding liquid culture medium, and culturing at 37 deg.C for 12 hr; and (3) diluting 100 mu L of the bacterial suspension by 10 times, putting 100 mu L of the bacterial suspension on a solid culture medium plate, and uniformly coating.
2. Preparing different gel samples A, A4, wherein A is the antibacterial peptide gynecological lotion of the invention, and the preparation is carried out according to the instruction; a4 the method of the invention eliminates the need of placing the bees in the environment of Gardnerella and Bacteroides for culturing to obtain the lotion in step 1).
3. And (3) beating the filter paper into a circular sheet with the radius of 3mm by using a perforating machine, sterilizing for 30min by using high-pressure steam at 120 ℃, and drying to obtain the sterile filter paper sheet.
4. Different gel samples A, B, C were applied to the surface of the aseptic filter paper sheet, the thickness of the applied gel samples was 10 μm, the applied surface was attached to a bacteria-containing plate, the plate was cultured at 37 ℃ for 12 hours, and the radius of the zone of inhibition of the filter paper sheet was measured, the results are shown in Table 3.
TABLE 3 radius of inhibition zone (mm) of each bacterium of lotion prepared by different methods
Gel sample Gardnerella sp Bacteroides Lactobacillus strain Vaginal bifidobacteria
A 8.3 7.2 2.9 2.6
A4 5.8 4.7 3.3 3.4
As can be seen from Table 3, the bees are cultured in an environment similar to vaginitis and containing gardnerella and bacteroides, and the bees are stimulated to secrete a large amount of antibacterial peptides resisting the gardnerella and the bacteroides, and the antibacterial peptide components can kill pathogenic flora (gardnerella and bacteroides), create healthy and mild growth and reproduction conditions for beneficial flora lactobacillus, and adjust the imbalance of vaginal flora balance in the environment of vaginitis.
Fourth, use test
The antibacterial peptide component of the product is beneficial to smooth and local absorption of vagina, has no irritation, is insisted on proper use for 2-4 cycles (1 week is a cycle, and the use is carried out for 3 times per cycle), obviously relieves vaginitis, reduces secretion, causes no pruritus and burning sensation at vulva, and eliminates leucorrhea peculiar smell.

Claims (6)

1. The preparation method of the antibacterial peptide gynecological lotion is characterized by comprising the following steps:
1) placing bees in an environment containing gardnerella and anaerobes for 1-2 h, heating at 50-70 deg.C for 1-2 h, oven drying the whole bees, adding radix Glycyrrhizae tablets, and pulverizing to obtain bee powder; the mass ratio of the liquorice tablets to the bees is 1: 8-1: 10;
2) putting the bee powder into a glycerol-acetic acid solution containing glucose, soaking for 30-40 h, and then ultrasonically vibrating for 10-20 min to obtain a mixed solution;
3) filtering the mixed solution to remove filter residue, and freeze-drying the filtrate to obtain bee extract;
4) adding the bee extract and vitamin C into cucumber juice, stirring at 30-50 ℃ for 20-40 min, and adding 0.6-1.2 mmol/L MgSO4Stirring the solution to obtain the solution; the mass concentration of the cucumber juice is 5-10%; the components in the step 4) are prepared according to the following proportions in parts by mass: 4-8 parts of bee extract,30-50 parts of cucumber juice, 1-3 parts of vitamin C and MgSO (MgSO)4And 3-5 parts of a solution.
2. The method for preparing gynecological lotion containing antibacterial peptides according to claim 1, wherein said bees are chosen from Apis mellifera, Apis cerana, Apis anatoria, Apis gacalifornica, Apis caribbeloensis, and Apis karebauwana.
3. The method for preparing gynecological lotion containing antibacterial peptides according to claim 1, wherein the bee powder has a particle size of 1-5 mm.
4. The method for preparing the antibacterial peptide gynecological lotion according to claim 1, wherein the volume fraction of glycerol in the glycerol-acetic acid solution is 15-30%.
5. The method for preparing the antibacterial peptide gynecological lotion according to claim 1, wherein the concentration of glucose in the glycerol-acetic acid solution is 0.5-1 mol/L.
6. The preparation method of the antibacterial peptide gynecological lotion according to claim 1, wherein the step 3) of freeze-drying is to freeze at-30 to-10 ℃ and then to dehydrate under 2 to 10Pa of vacuum.
CN201711212780.6A 2017-11-28 2017-11-28 Preparation method of antibacterial peptide gynecological lotion Expired - Fee Related CN107714733B (en)

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CN1213761C (en) * 2002-04-22 2005-08-10 武汉大学 Nano woman's cleaning and nursing aloe lotion and its preparing method
CN102048675A (en) * 2010-12-15 2011-05-11 郑高育 Fragrant grass vagina cleaning liquid and preparation process thereof
CN103083472A (en) * 2013-01-25 2013-05-08 北海光谷纳米科技研发中心 Nano silver anti-inflammatory gel for treating gynecologic inflammation and preparation method thereof
CN105267472A (en) * 2015-01-15 2016-01-27 高淑萍 Traditional Chinese medicine medicament for treating gynecological diseases and applications thereof
CN106146608A (en) * 2015-03-16 2016-11-23 江西科诺生物科技有限公司 A kind of extraction preparation method of apidaecin
CN107056913A (en) * 2017-03-21 2017-08-18 济南大学 A kind of method for preparing melittin
CN107149619A (en) * 2017-05-18 2017-09-12 四川悦艾健康科技有限公司 Harmful bacteria breeding can be suppressed and the compound preparation of growth of probiotics can be promoted

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