CN107624539B - Culture medium and culture method for planting bulrush by utilizing artificial floating island - Google Patents

Culture medium and culture method for planting bulrush by utilizing artificial floating island Download PDF

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CN107624539B
CN107624539B CN201711026168.XA CN201711026168A CN107624539B CN 107624539 B CN107624539 B CN 107624539B CN 201711026168 A CN201711026168 A CN 201711026168A CN 107624539 B CN107624539 B CN 107624539B
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floating island
artificial floating
polyethylene glycol
culture
culture medium
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CN107624539A (en
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周立彪
董艳兵
方苏
闫兴富
曹君迈
宋海丽
吴锋
杜梦
何磊
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North Minzu University
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Abstract

The invention discloses a culture medium for planting reeds by utilizing an artificial floating island, which comprises a polyethylene glycol mixed liquid matrix and a simulated soil solid matrix, wherein the simulated soil solid matrix consists of expanded vermiculite and sawdust; the polyethylene glycol mixed liquid matrix is prepared from polyethylene glycol, a NaCl aqueous solution and biological bacterial manure; provides a preparation method of the culture medium for planting the reeds by utilizing the artificial floating island. The invention also provides a culture method for planting reeds by utilizing the artificial floating island. The culture medium for planting the reeds improves the germination rate of the reed seeds by more than 10 percent, thereby more effectively improving the water body purification capacity, and leading the artificial floating island to have important significance in the aspect of river water eutrophication pollution control technology and certain popularization and application value.

Description

Culture medium and culture method for planting bulrush by utilizing artificial floating island
The technical field is as follows:
the invention relates to a method for planting bulrush, in particular to a culture medium and a culture method for planting bulrush by using an artificial floating island.
Background art:
the reed is a common aquatic plant, has a developed fibrous root system like other plants in the family Poaceae, and has two types of stems, namely an upright stem and a rhizome. The root-like stem has multiple nodes, can produce multiple tillering strips, and the upright stem obviously has two parts of nodes and internodes (the stem is often specially called as stalk in gramineae; called as pole in bamboo for distinguishing from gramineae); the internodes are hollow, usually cylindrical or slightly flat, and the medulla part is attached to the inner wall of the cavity, but the cavity is filled with the medulla part to make the internodes solid; the transverse partition plate is arranged in one section, so that the section is closed, the sheath ring and the stalk ring above the sheath can be seen from the outer surface, the vertical distance between the two rings of the same section can be called as the section, and the stalk bud is grown in the section. The reeds are grown on the banks of rivers, lakes, ponds and ditches and in low wetlands. Besides seed propagation, a large number of reeds are usually propagated in a rhizome tillering mode, dozens of plants of a reed cluster structure can be formed in one year, and rhizomes and fibrous roots grown from newly-grown tillering plants are interlaced and interwoven to form a complete root system structure which is very firm, so that an independent reed community can be formed in 1-3 years.
Under natural conditions, the germination rate of reed seeds is lower than 1 percent generally, but the quantity of the reed seeds is larger, so that the propagation of wild reeds is not influenced, and the germination rate of the reed seeds needs to be improved relatively if artificial propagation is carried out. The method is characterized in that buoys are constructed by using bulrush as a main plant, the buoys can float in water, and rhizomes and fibrous roots grow in a staggered mode to form a firm integral structure so that the buoys do not sink and fall. The reed root system provides a special aquatic habitat for inhabitation of various microorganisms, protozoa, phytoplankton and the like to form a small ecological system, has an efficient water quality purification function, can absorb heavy metals and other pollutants, thereby improving the purification rate of the water body and having certain economic value. However, the cutting of the living reed is generally suitable for the water depth below 0.5m because of the influence of the water depth, cannot achieve the expected target, and has high difficulty, labor and time consumption in transferring. Based on the above, the applicant proposes an artificial floating island constructed by using reed as a main plant in a seed propagation manner to solve the above problems. Therefore, in order to combine the constructed artificial floating island to plant the reed in a seed propagation manner, improve the germination rate and improve the purification rate of the water body, a culture medium and a culture method for planting the reed by using the artificial floating island are urgently needed.
The invention content is as follows:
the invention aims to provide a culture medium and a culture method for planting bulrush by utilizing an artificial floating island.
In order to achieve the purpose, the invention adopts the following technical scheme:
the culture medium for planting the reeds by utilizing the artificial floating island comprises a polyethylene glycol mixed liquid matrix and a simulated soil solid matrix, wherein: the simulated soil solid matrix consists of expanded vermiculite and sawdust; the polyethylene glycol mixed liquid matrix is prepared from polyethylene glycol, NaCl aqueous solution and biological bacterial manure. Wherein:
selecting the material with the grain diameter of 120 meshes and the specific gravity of 0.18g/cm3Expanded vermiculite and specific gravity of 0.2g/cm3The sawdust of (1);
the polyethylene glycol adopts 10% polyethylene glycol water solution (PEG6000) as water potential control agent;
the NaCl aqueous solution is 1mol NaCl aqueous solution for controlling salt content.
The preparation method of the culture medium for planting the reeds by utilizing the artificial floating island comprises the following steps:
1) proportioning: weighing the raw materials according to a certain ratio, wherein the raw materials comprise the following components in percentage: 50% of expanded vermiculite, 50% of sawdust, 10% of polyethylene glycol aqueous solution and the solid matrix in the solid matrix are in a ratio of 1:0.9, and 10% of biological bacterial fertilizer and the solid matrix are in a ratio of 1: 0.1;
2) and (3) sterilization: mixing the weighed expanded vermiculite with sawdust, sterilizing at high temperature for 50min, and cooling to a thickness for later use; adjusting salinity to 0.25% with 10% polyethylene glycol aqueous solution and 1mol NaCl aqueous solution, and then adding NaH2PO4-Na2HPO4Adjusting pH value to 7.5 with buffer solution, sterilizing at high temperature for 20min, cooling, and adding 10% biological bacterial fertilizer. And mixing the solid phase and the liquid phase after sterilization and cooling to obtain the complete culture medium.
Further, in order to improve the germination rate of the seeds, a soil matrix with the pH value of 7.5 is selected, and the germination rate is optimal under the conditions that the salt content is 0.25 percent and the soil penetration type is about-0.3 Mpa.
The culture method for planting bulrush by using the artificial floating island comprises the following steps:
1) mixing the prepared culture medium with the treated reed seeds, adding 10% polyethylene glycol aqueous solution to stably mix the solid phase and the liquid phase to obtain a seed-mixed culture material; wherein: mixing 10 reed seeds into each cubic centimeter of culture medium based on the volume of the solid matrix;
2) injecting the mixed culture material into a hollow floating pipe of the artificial floating island, and sealing openings at two ends by using wood plugs;
3) germinating the seeds at 25 deg.C under semi-shading condition (seedling can be grown in greenhouse or indoor in early spring), and transferring to outdoor after the seeds start germinating for 7-10 days and the seedlings grow to about 10 cm high;
4) installing a hollow floating pipe moved outdoors on a central pillar of an artificial floating island, fixing the artificial floating island in shoreside water to a proper depth, hardening seedlings for 45 days under the conditions of semi-shallow water and semi-shading, and discontinuously removing weak seedlings and dry seedlings in the hardening process;
5) after the reed seedlings grow densely, the artificial floating island is separated from the fixing piles and is arbitrarily dragged in water, and the whole culture process is finished.
The above reed seeds are treated by inducing germination at-4 deg.C in dark for 2 weeks, and storing in shade at normal temperature for 1 month.
Further, the salt concentration of the seed dressing mixed culture material in the seed germination process is optimally controlled within the range of 0.1-0.25%.
The artificial floating island is used for planting the reeds, the hollow floating pipe device fixed on the central pillar of the artificial floating island is used as a carrier for propagation and growth of reed seeds, the seed germination process is not influenced by the depth of a water body on water, and different requirements can be met for fixing plants.
The specific implementation mode is as follows:
the technical solution of the present invention will be described in detail with reference to specific examples.
Example 1: the culture method for planting the reeds by utilizing the artificial floating island specifically comprises the following steps:
1. the preparation of the culture medium for planting the reed comprises the following steps:
1) proportioning: weighing the raw materials according to a certain ratio, wherein the raw materials comprise the following components in percentage: 50% of expanded vermiculite, 50% of sawdust, 10% of polyethylene glycol aqueous solution and the solid matrix in the solid matrix are in a ratio of 1:0.9, and 10% of biological bacterial fertilizer and the solid matrix are in a ratio of 1: 0.1;
2) and (3) sterilization: mixing the weighed expanded vermiculite with sawdust, sterilizing at high temperature for 50min, and cooling to a thickness for later use; adjusting salinity to 0.25% with 10% polyethylene glycol aqueous solution and 1mol NaCl aqueous solution, and then adding NaH2PO4-Na2HPO4Adjusting pH value to 7.5 with buffer solution, sterilizing at high temperature for 20min, cooling, and adding 10% biological bacterial fertilizer. And mixing the solid phase and the liquid phase after sterilization and cooling to obtain the complete culture medium.
2. Seed treatment
After inducing germination at-4 deg.C for 2 weeks in the dark, keeping in shade at normal temperature, and storing for 1 month. The germination experiment of the treated reed seeds is carried out by the following specific method:
1) polyethylene glycol as culture medium: set up 2 treatment levels of water content and salinity, set up 3 parallels under every level:
a. water content: the water content was set at 30%, 40%, 50%, 60%, 70%. The preparation method of the substrate taking the water content of 30% as an example is as follows: 1000ml of saline solution with the salinity of 0.2% is prepared and is filled in a triangular flask for preparing a substrate and supplementing water at the later stage to ensure the soil humidity, and the saline solution is not enough to be prepared. 7g of polyethylene glycol and 3ml of 0.2% saline solution are weighed and mixed uniformly in a culture dish, 100 seeds are uniformly scattered in the dish, and a dish cover is covered. As for the incubation in an incubator at 10 ℃ the seeds were supplied with the appropriate growth temperature for the experiments carried out in winter at that time.
b. Salt content: the salt content is set to be 0.05%, 0.15%, 0.2%, 0.25%, 0.3%. Wherein the water content of each salt concentration setting is 30%, 5 parts of salt solution with the salinity of 0.05%, 0.15%, 0.2%, 0.25% and 0.3% are respectively prepared, and the salt solution is respectively filled in a triangular flask for preparing a matrix and supplementing water at the later stage to ensure the soil humidity and is not enough to be prepared. The preparation method of the substrate taking the salt concentration of 0.05% as an example is as follows: weighing 7g of polyethylene glycol and 3ml of 0.05% saline solution, uniformly mixing the two solutions in a culture dish, uniformly scattering 100 seeds in the dish, covering the dish cover, and placing the dish cover in a constant-temperature incubator at 20 ℃ for constant-temperature culture.
2) Taking soil as a culture medium: set up 2 treatment levels of water content and salinity, set up 3 parallels under every level:
a. water content: soil moisture was controlled by controlling the time interval between two watering. 3 watering time intervals were set: 1 day/time, 2 days/time, 3 days/time, and ensuring soil moistening during each watering. And (3) uniformly scattering 100 seeds in the half-dish treated moist soil, covering a little soil on the seeds, and covering the dish cover. The culture can be carried out in the sunny place at the bed (since the culture starts to be prepared in winter and spring, the temperature at the window after treatment is the proper growth temperature).
b. Salt content: the salt content is set to be 0.05%, 0.15%, 0.2%, 0.25%, 0.3%. The water content of each salinity setting is only the soil moistening, 5 parts of salt solution with the salinity of 0.05 percent, 0.15 percent, 0.2 percent, 0.25 percent and 0.3 percent are respectively prepared, the salt solution with the salinity of 1000ml is sequentially filled in a triangular flask for preparing a substrate and supplementing water in the later period to ensure the soil humidity, and the salt solution is not enough to be prepared. The preparation method of the substrate taking the salt concentration of 0.05% as an example is as follows: uniformly spreading 100 seeds in a dish from half dish of treated dry soil, covering a little soil on the seeds, sucking the solution from the solution with corresponding salinity concentration by using a rubber head dropper, watering the soil until the soil is wet, covering a dish cover, and placing the dish cover at the edge of a bed to be exposed to the sun for cultivation.
3) The experimental results are as follows: the salt and water control of the reed seeds under different storage conditions on two substrates respectively is carried out for germination experiments, and the results are obtained:
a. normal room temperature preservation of seeds: the germination rate is lower than 1%.
b. Low-temperature induction treatment of seeds: the water control has no obvious difference, and the germination rate is lower than 1 percent; under the condition of salt control, the germination rate is improved to about 10 percent when the salt content is between 0.1 and 0.25 percent.
c. And (3) storing the seeds in the dark at normal temperature: the germination rate is lower than 1%.
3. The culture method for planting bulrush by using the artificial floating island comprises the following steps:
1) mixing the prepared culture medium with the treated reed seeds, adding 10% polyethylene glycol aqueous solution to stably mix the solid phase and the liquid phase to obtain a seed-mixed culture material; wherein: mixing 10 reed seeds into each cubic centimeter of culture medium based on the volume of the solid matrix;
2) injecting the mixed culture material into a hollow floating pipe of the artificial floating island, and sealing openings at two ends by using wood plugs;
3) germinating the seeds at 25 deg.C under semi-shading condition (seedling can be grown in greenhouse or indoor in early spring), and transferring to outdoor after the seeds start germinating for 7-10 days and the seedlings grow to about 10 cm high;
4) installing a hollow floating pipe moved outdoors on a central pillar of an artificial floating island, fixing the artificial floating island in shoreside water to a proper depth, hardening seedlings for 45 days under the conditions of semi-shallow water and semi-shading, and discontinuously removing weak seedlings and dry seedlings in the hardening process;
5) after the reed seedlings grow densely, the artificial floating island is separated from the fixing piles and is arbitrarily dragged in water, and the whole culture process is finished.

Claims (1)

1. A culture method for planting bulrush by using an artificial floating island is characterized by comprising the following steps: the method comprises the following steps:
1) mixing the prepared culture medium for planting reeds by utilizing the artificial floating island with the treated reed seeds, and adding 10% of polyethylene glycol aqueous solution to stably mix the solid phase and the liquid phase to obtain a seed-mixed culture material; mixing the prepared culture medium with the treated reed seeds, wherein the volume of the solid matrix is taken as a standard, and 10 reed seeds are mixed in each cubic centimeter of the culture medium; the treatment of the reed seeds comprises inducing germination for 2 weeks at-4 ℃ under dark condition, and keeping for 1 month at normal temperature in a shady and dry way;
the culture medium for planting the reeds by utilizing the artificial floating island comprises a polyethylene glycol mixed liquid matrix and a simulated soil solid matrix, wherein the simulated soil solid matrix consists of expanded vermiculite and sawdust; the polyethylene glycol mixed liquid matrix is prepared from polyethylene glycol, a NaCl aqueous solution and biological bacterial manure; the solid matrix of the simulated soil is prepared from expanded vermiculite with the particle size of 120 meshes and the specific gravity of 0.18g/cm3 and expanded vermiculite with the specific gravity of 0.2g/cm3The sawdust of (1); polyethylene glycol adopts 10% polyethylene glycol aqueous solution as water potential control agent; the NaCl aqueous solution adopts 1mol of NaCl aqueous solution for controlling salinity; the soil matrix is pH 7.5, has salt content of 0.25%, and has a soil penetration of-0.3 Mpa;
the preparation method of the culture medium for planting the reeds by utilizing the artificial floating island comprises the following steps:
a. proportioning: weighing the raw materials according to a certain ratio, wherein the raw materials comprise the following components in percentage: 50% of expanded vermiculite, 50% of sawdust, 10% of polyethylene glycol aqueous solution and the solid matrix in the solid matrix are in a ratio of 1:0.9, and 10% of biological bacterial fertilizer and the solid matrix are in a ratio of 1: 0.1;
b. and (3) sterilization: mixing the weighed expanded vermiculite with sawdust, sterilizing at high temperature for 50min, and cooling for later use; adjusting salinity to 0.25% with 10% polyethylene glycol aqueous solution and 1mol NaCl aqueous solution, and then adding NaH2PO4-Na2HPO4Adjusting the pH value of the buffer solution to 7.5, sterilizing at high temperature for 20min, and adding 10% biological bacterial fertilizer; mixing the solid phase and the liquid phase after sterilization and cooling to obtain a complete culture medium;
2) injecting the mixed culture material into a hollow floating pipe of the artificial floating island, and sealing openings at two ends by using wood plugs;
3) germinating the seeds at 25 ℃ under the condition of semi-shading, and moving the seeds to the outdoor after the seeds begin to germinate and seedlings grow to be about 10 cm high after 7-10 days; the salt concentration of the seed-dressing mixed culture material is controlled within the range of 0.1-0.25% in the seed germination process;
4) installing a hollow floating pipe moved outdoors on a central pillar of an artificial floating island, fixing the artificial floating island in shoreside water to a proper depth, hardening seedlings for 45 days under the conditions of semi-shallow water and semi-shading, and discontinuously removing weak seedlings and dry seedlings in the hardening process;
5) after the reed seedlings grow densely, the artificial floating island is separated from the fixing piles and is arbitrarily dragged in water, and the whole culture process is finished.
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