CN107541371A

CN107541371A - A kind of preparation method of Fructus Akebiae compound fruit wine

Info

Publication number: CN107541371A
Application number: CN201710978418.3A
Authority: CN
Inventors: 王伟; 刘春生; 蒋宣斌; 吴晓龙; 张正文; 林梅; 史秀玲; 田松; 张玉娟; 王晓君
Original assignee: CHONGQING ACADEMY OF FORESTRY
Current assignee: CHONGQING ACADEMY OF FORESTRY
Priority date: 2017-10-18
Filing date: 2017-10-18
Publication date: 2018-01-05

Abstract

The invention discloses a kind of preparation method of Fructus Akebiae compound fruit wine, including step is brewageed in the pre-treatment of raw material, the adjustment of composition, the activation of yeast and compound fruit wine, in preparation process by Fructus Akebiae and grape according to 1:1~1:5 mass ratio mixing is broken into mixing pulp, mixing pulp is being sterilized into 30min at 68 DEG C, sodium hydrogensulfite is added after cooling, it is inoculated with activated yeast, fermented at 18~25 DEG C, the residual sugar amount in a wine liquid is determined every 12h, when residual sugar amount, which continues 2d, keeps constant, with micro porous filtration membrane filtration, that is, the compound fruit wine clarified.Fructus Akebiae compound fruit wine prepared by the present invention has the aroma of strong Fructus Akebiae and grape, the pleasing coordination of outward appearance, clear, full color, mouthfeel is soft full, sour-sweet appropriate, and wine volume property is stable, phenomena such as storage cycle length is not in muddy, sedimentation and brown stain.

Description

A kind of preparation method of Fructus Akebiae compound fruit wine

Technical field

The present invention relates to fruit wine processing technique field, more particularly to a kind of preparation method of Fructus Akebiae compound fruit wine.

Background technology

Fructus Akebiae (Akebia trffoIiate) is Lardizabalaceae, threeleaf akebia category, is perennial half evergreen fallen leaves winding rattan This plant, fruit is from the appearance oval or kidney type, because it is in lunar calendar August or so maturation, it is ripe after fruit it is overall Prolong belly to split, also known as August among the people fries, YANGKAIKOU, HUOXUETENG etc..Fructus Akebiae is mainly grown on China and Japan, country's plantation Distribution is especially extensive, and Fructus Akebiae is more based on wild, and main growth distribution is in the Yangtze river basin, Southern Shaanxi, the Gansu southeast The areas such as portion, Southern Henan and Shanxi south.It is suitable warm compared with shade tolerant, slightly chilly as a kind of temperate zone and subtropical plant And the environmental condition moistened, it is desirable to which draining is good, and humus content is than more rich yellow earth.

Fructus Akebiae pulp is creamy white, Fresh ＆ Tender in Texture, and special taste, the smell of fruits is very sweet, there is the laudatory title of " wild banana ".As one Kind ecological ideas health fruit, nutritive value is extremely abundant, and research is found, soluble sugar content in every 100g Fructus Akebiae fresh fruits 2.2g, Vitamin C content 0.91mg, fat content 45mg, protein content 1.342g, contained protein amino acid content is compared with apple Amino acid in fruit, pears, Kiwi berry is complete, and 8 kinds of amino acid contents are 567mg/100g wherein necessary to growth in humans.Fruit The micro elements needed by human such as middle Fe, Zn, Ca, Mg, Mn content higher than apple, pear and other fruits, be a kind of content of mineral substances extremely Abundant fruit resource.Fructus Akebiae integrates nutrition and health care and is referred to as " health-care fruit king ", according to《Chinese herbal medicine dictionary》Note Carry, fruit can be eaten raw and make wine, and having improves body digestive system, remove unnecessary rubbish in vivo, promote human normal blood to follow The magical effect such as ring, long-term consumption can not only reduce wrinkle of skin and precipitation facial colour spot pigment, reach the effect of beautifying face and moistering lotion Fruit.And also anti-inflammatory analgetic, dehumidifying diuresis, tonifying kidney and nourishing liver, treatment impotence, the effect of relaxing bowel etc., have huge medicinal And economic prospect.

However, existing Fructus Akebiae compound fruit wine is to carry out the purification of fruit wine and clarification using fining agent, first, technique Complexity, efficiency is low, second, and the pH value of the proportioning and compound fruit wine of the dosage of fining agent and raw material is closely related, it is therefore, clear The dosage of clear agent is difficult control, and when the dosage of fining agent is excessive, excessive fining agent is dissolved in compound fruit wine, causes composite fruit The turbid phenomenon of wine, and influence wine and women-sensual pursuits, aroma etc., when the dosage of fining agent is very few, it is impossible to by the protein in compound fruit wine and The abundant flocculation sediment of tannin comes out, and equally causes the turbid phenomenon of compound fruit wine.

The content of the invention

In view of this, it is an object of the invention to provide a kind of preparation method of Fructus Akebiae compound fruit wine, make Fructus Akebiae compound The clarifying treatment technique of fruit wine is simple, efficiency high, and clarifying effect is good, and the compound fruit wine property after processing is stable.

The present invention solves above-mentioned technical problem by following technological means：

A kind of preparation method of Fructus Akebiae compound fruit wine, comprises the following steps:

Step 1, raw material pre-treatment：Selection maturity is good, have no mechanical damage, non-rot grape and quality are soft Soft, belly prolongs that linea ventralis splits, pulp is creamy white the Fructus Akebiae of transparence, according to 1:1~1:5 mass ratio mixing crushes To mixing pulp；

Step 2, the adjustment of composition：Take mixing pulp to add pectase in 0.8g/L ratio, 24h, root are digested in 50 DEG C 1%vol alcoholic strength is produced according to theoretical value 17g/L sucrose fermentation energy, the alcoholic strength required for guarantee, then will mix fruit with sucrose The total sugar content of juice is adjusted to >=204g/L, and pol is about 20~25 ° of Brix, then adjusts pH to 3.0~4.0 with citric acid, obtains To Mixed adjustment pulp；

Step 3, the activation of yeast：Dry ferment is taken in 0.25~0.35g of 1L Mixed adjustment pulps ratio, is added to 5% Glucose solution in, be well mixed, activate 30min in 35 DEG C of water bath with thermostatic control, when its aerogenesis bubble to be seen is abundant, produce To activated yeast；

Step 4, compound fruit wine are brewageed：Take Mixed adjustment pulp to sterilize 30min at 68 DEG C, pressed after cooling 0.006mg/L ratio adds sodium hydrogensulfite, is then inoculated with activated yeast, is fermented at 18~25 DEG C, and one is determined every 12h Residual sugar amount in secondary wine liquid, when residual sugar amount, which continues 2d, keeps constant, terminate fermentation, with micro porous filtration membrane filtration, that is, obtain clear Clear compound fruit wine.The effect that sodium hydrogensulfite suppresses enzymatic browning is preferable, can reduce enzymatic reaction intermediate quinone and press down The activity of polyphenol oxidase processed；Meanwhile sodium hydrogensulfite can also generate stable colourless thing with the intermediate product quinone of enzymatic reaction, Suppress the activity of polyphenol oxidase, and then prevent from suppressing the brown stain of compound fruit wine.

Further, the mass ratio of Fructus Akebiae and grape is 1 in step 1:2~1:3.

Further, the pH of Mixed adjustment pulp is 3.2~3.5 in rapid two.

Further, the fermentation temperature of Mixed adjustment pulp is 18~20 DEG C in step 4.

Further, the aperture of the micropore filtering film in step 4 is 0.18~0.22 μm, and its preparation method is as follows：

1) take hectorite to be configured to 2wt% hectorite colloidal solution with deionized water, add 0.4~0.6 times of hectorite The cetyl trimethylammonium bromide of quality, the stirring reaction 6h under 80 DEG C of water-baths, add the shells of 2~3 times of quality of hectorite Glycan, continue after reacting 6h, stand 2h, be centrifuged off supernatant, precipitation deionized water rinsing to neutrality, then drying, Grinding, obtains hectorite/cetyl trimethylammonium bromide/Chitosan powder；

2) polypropylene is taken in reactor, the adipic acid of addition 0.2~0.4 times of quality of polypropylene, and polypropylene 2.1~ The soybean oil of 2.3 times of quality, in 150~160 DEG C, stirred under nitrogen atmosphere 3h, homogeneous phase solution is obtained, is then cooled to 70~80 DEG C, the hectorite/cetyl trimethylammonium bromide/chitosan obtained in being added 1) in the ratio of homogeneous phase solution quality 5~8% Powder, after being well mixed under 1500~2000r/min rotating speed, standing and defoaming, until casting solution deaeration is complete, obtain casting film Liquid；

3) casting solution is taken to be transferred quickly to be film-made in knifing machine, wherein quenching temperature is 35~45 DEG C, and scraper temperature is 200 DEG C, in film-forming process, casting solution temperature reduces, and solution occurs liquid-liquid and is separated, and forms polypropylene concentrated phase and soybean oil is dilute The interpenetrating structure of phase, then extracts 24h by obtained film in 25~35 DEG C of n-hexane, and diluent soybean oil is extracted Afterwards, soybean oil takes up space as fenestra, obtains micropore filtering film；

Further, micropore filtering film also have passed through hydrophilic modification, and hydrophilic modification method is as follows：

By micropore filtering film acetone extraction 12h, 6h is dried in vacuo, ozone reactor is then placed in and carries out ozone processing 30 ~60min, PP microporous barriers are aoxidized, and then introduce peroxide, vacuum outgas 30min after the completion of ozone processing, removed The O of micropore filtering film absorption₂And O₃, then with ethanol soak 10min, be then immersed in the mixing of iron ammonium sulfate and acrylic monomers In the aqueous solution, letting nitrogen in and deoxidizing, 1~2h is reacted at 45 DEG C, make acrylic monomers and peroxide grafted, take out micropore filtering film 12h is extracted with methanol, then is cleaned for several times with water, to remove homopolymer, monomer and iron ammonium sulfate, 6h is dried in vacuo, is changed Property micropore filtering film.After micropore filtering film is treated by ozone, its Surface Creation peroxide (POOH, P represent high polymer), adding In the case of heat, peroxide resolves into PO and HO free radicals, and PO can trigger monomer that graft copolymerization, HO occurs Monomer can be triggered to generate homopolymer, and the Fe in iron ammonium sulfate²⁺The generation of HO free radicals can be prevented, and then suppresses homopolymer Generation.

Further, the standing and defoaming in step 2) is in 70~80 DEG C of temperature, 0.090~0.095MPa of vacuum vacuum bar Carried out under part.

Further, vacuum drying condition is：40~45 DEG C of temperature, 0.09~0.10MPa of vacuum.

Further, in the mixed aqueous solution of iron ammonium sulfate and acrylic monomers, the body of acrylic monomers and deionized water Product is than being 1:3, the mol ratio of iron ammonium sulfate and acrylic monomers is 1.2:1.

Beneficial effects of the present invention：

1. Fructus Akebiae compound fruit wine prepared by the present invention has the aroma of strong Fructus Akebiae and grape, the pleasing association of outward appearance Tune, clear, full color, mouthfeel is soft full, sour-sweet appropriate, and wine volume property is stable, and storage cycle length, is not in muddy Phenomena such as turbid, sedimentation and brown stain；

2. the present invention reaches clarification, purifies the purpose of compound fruit wine, composite fruit by micro porous filtration membrane filtration compound fruit wine The impurity such as the pulp, fiber, saccharomycete, miscellaneous bacteria and the protein that are remained in wine remove it is abundant, whole process without using fining agent, Clarification process is simple and easy to control, avoids influence of the fining agent improper use to wine and women-sensual pursuits, aroma and mixed degree；

3. micropore filtering film prepared by the present invention has good hydrophily, film surface is easily readily wetted by water, the flux of film Greatly, strainability is good, and surface is not easy to adsorb hydrophobic and amphoteric solute, is easy to recover original performance through over cleaning film, makes micro- Hole filter membrane can reuse, and the utilization rate of film is high.

Embodiment

Below with reference to embodiment, the present invention is described in detail：

The preparation embodiment one of micropore filtering film

Take hectorite to be configured to 2wt% hectorite colloidal solution with deionized water, add the ten of 0.4 times of quality of hectorite Six alkyl trimethyl ammonium bromides, the stirring reaction 6h under 80 DEG C of water-baths, the chitosan of 2 times of quality of hectorite is added, is continued anti- After answering 6h, 2h is stood, supernatant is being centrifuged off, precipitation deionized water rinsing to neutrality, is then drying, ground, obtain lithium Saponite/cetyl trimethylammonium bromide/Chitosan powder；Polypropylene is taken in reactor, adds 0.2 times of quality of polypropylene Adipic acid, and the soybean oil of 2.1 times of quality of polypropylene, in 150~160 DEG C, stirred under nitrogen atmosphere 3h, are mixed Liquid, 70~80 DEG C are then cooled to, the hectorite/cetyl three obtained in being added 1) in the ratio of homogeneous phase solution quality 5% Methyl bromide ammonium/Chitosan powder, after being well mixed under 1500~2000r/min rotating speed, in 70~80 DEG C, 0.090~ 0.095MPa vacuum condition standing and defoaming, until casting solution deaeration is complete, obtain casting solution；Casting solution is transferred quickly to scrape Be film-made in film machine, wherein quenching temperature be 35 DEG C, scraper temperature be 200 DEG C, then by obtained film in 25~35 DEG C just 24h is extracted in hexane, after diluent soybean oil is extracted, soybean oil takes up space as fenestra, obtains micropore filtering film；Will be micro- Hole filter membrane acetone extraction 12h, in 40~45 DEG C, 6h is dried in vacuo under conditions of 0.09~0.10MPa, is then placed in ozone Reactor carries out ozone processing 30min, vacuum outgas 30min after the completion of ozone processing, removes the O of micropore filtering film absorption₂With O₃, then with ethanol soak 10min, be then immersed in the mixed aqueous solution of iron ammonium sulfate and acrylic monomers, wherein, acrylic acid The volume ratio of monomer and deionized water is 1:3, the mol ratio of iron ammonium sulfate and acrylic monomers is 1.2:1, letting nitrogen in and deoxidizing, in 1h is reacted at 45 DEG C, makes acrylic monomers and peroxide grafted, micropore filtering film is taken out and extracts 12h with methanol, then it is clear with water Wash for several times, in 40~45 DEG C, 6h is dried in vacuo under conditions of 0.09~0.10MPa, obtains modified micropore filter membrane.

The preparation embodiment two of micropore filtering film

Take hectorite to be configured to 2wt% hectorite colloidal solution with deionized water, add the ten of 0.6 times of quality of hectorite Six alkyl trimethyl ammonium bromides, the stirring reaction 6h under 80 DEG C of water-baths, the chitosan of 3 times of quality of hectorite is added, is continued anti- After answering 6h, 2h is stood, supernatant is being centrifuged off, precipitation deionized water rinsing to neutrality, is then drying, ground, obtain lithium Saponite/cetyl trimethylammonium bromide/Chitosan powder；Polypropylene is taken in reactor, adds 0.4 times of quality of polypropylene Adipic acid, and the soybean oil of 2.3 times of quality of polypropylene, in 150~160 DEG C, stirred under nitrogen atmosphere 3h, are mixed Liquid, 70~80 DEG C are then cooled to, the hectorite/cetyl three obtained in being added 1) in the ratio of homogeneous phase solution quality 8% Methyl bromide ammonium/Chitosan powder, after being well mixed under 1500~2000r/min rotating speed, in 70~80 DEG C, 0.090~ 0.095MPa vacuum condition standing and defoaming, until casting solution deaeration is complete, obtain casting solution；Casting solution is transferred quickly to scrape Be film-made in film machine, wherein quenching temperature be 45 DEG C, scraper temperature be 200 DEG C, then by obtained film in 25~35 DEG C just 24h is extracted in hexane, after diluent soybean oil is extracted, soybean oil takes up space as fenestra, obtains micropore filtering film；Will be micro- Hole filter membrane acetone extraction 12h, in 40~45 DEG C, 6h is dried in vacuo under conditions of 0.09~0.10MPa, is then placed in ozone Reactor carries out ozone processing 60min, vacuum outgas 30min after the completion of ozone processing, removes the O of micropore filtering film absorption₂With O₃, then with ethanol soak 10min, be then immersed in the mixed aqueous solution of iron ammonium sulfate and acrylic monomers, wherein, acrylic acid The volume ratio of monomer and deionized water is 1:3, the mol ratio of iron ammonium sulfate and acrylic monomers is 1.2:1, letting nitrogen in and deoxidizing, in 2h is reacted at 45 DEG C, makes acrylic monomers and peroxide grafted, micropore filtering film is taken out and extracts 12h with methanol, then it is clear with water Wash for several times, in 40~45 DEG C, 6h is dried in vacuo under conditions of 0.09~0.10MPa, obtains modified micropore filter membrane.

The preparation embodiment three of micropore filtering film

Take hectorite to be configured to 2wt% hectorite colloidal solution with deionized water, add the ten of 0.5 times of quality of hectorite Six alkyl trimethyl ammonium bromides, the stirring reaction 6h under 80 DEG C of water-baths, the chitosan of 2.5 times of quality of hectorite is added, is continued After reacting 6h, 2h is stood, supernatant is being centrifuged off, precipitation deionized water rinsing to neutrality, is then drying, ground, obtain Hectorite/cetyl trimethylammonium bromide/Chitosan powder；Polypropylene is taken in reactor, adds 0.3 times of quality of polypropylene Adipic acid, and the soybean oil of 2.2 times of quality of polypropylene in 150~160 DEG C, stirred under nitrogen atmosphere 3h, mixed Liquid, 70~80 DEG C are then cooled to, the hectorite/cetyl three obtained in being added 1) in the ratio of homogeneous phase solution quality 6% Methyl bromide ammonium/Chitosan powder, after being well mixed under 1500~2000r/min rotating speed, in 70~80 DEG C, 0.090~ 0.095MPa vacuum condition standing and defoaming, until casting solution deaeration is complete, obtain casting solution；Casting solution is transferred quickly to scrape Be film-made in film machine, wherein quenching temperature be 40 DEG C, scraper temperature be 200 DEG C, then by obtained film in 25~35 DEG C just 24h is extracted in hexane, after diluent soybean oil is extracted, soybean oil takes up space as fenestra, obtains micropore filtering film；Will be micro- Hole filter membrane acetone extraction 12h, in 40~45 DEG C, 6h is dried in vacuo under conditions of 0.09~0.10MPa, is then placed in ozone Reactor carries out ozone processing 45min, vacuum outgas 30min after the completion of ozone processing, removes the O of micropore filtering film absorption₂With O₃, then with ethanol soak 10min, be then immersed in the mixed aqueous solution of iron ammonium sulfate and acrylic monomers, wherein, acrylic acid The volume ratio of monomer and deionized water is 1:3, the mol ratio of iron ammonium sulfate and acrylic monomers is 1.2:1, letting nitrogen in and deoxidizing, in 1.5h is reacted at 45 DEG C, makes acrylic monomers and peroxide grafted, micropore filtering film is taken out and extracts 12h with methanol, then use water Cleaning for several times, in 40~45 DEG C, is dried in vacuo 6h, obtains modified micropore filter membrane under conditions of 0.09~0.10MPa.

The grafting degree of the micropore filtering film that Example one is prepared to embodiment three, respectively test microvia filter membrane (%), aperture (μm) and porosity (%), test result is as shown in table 1：

Table 1

Embodiment	Grafting degree (%)	Aperture (μm)	Porosity (%)
				Embodiment one	45.3	0.18	55.2
Embodiment two	50.2	0.22	60.9
				Embodiment three	46.5	0.19	57.6

From the data analysis of table 1, the grafting degree of micropore filtering film surface acrylic prepared by the present invention more than 45%, Aperture is 0.18~0.22 μm, and porosity is up to more than 55%.Therefore, the micropore filtering film that prepared by the present invention has good parent Water-based, whom film surface easily moistened by, and the flux of film is big, strainability is good, and surface is not easy to adsorb hydrophobic and amphoteric solute, warp Over cleaning film is easy to recover original performance, and the utilization rate of film is high.

The micropore filtering film that Example one is prepared to embodiment three, 1cm × 1cm diaphragm is respectively cut into, adopts With Japanese JIS Z2801:2010 standards carry out anti-microbial property detection, and every diaphragm distinguishes Parallel testing three times, and according to standard Its logarithm value is taken, when antibacterial value is more than or equal to 2, illustrates that antibiotic rate is more than or equal to 99%.The result of detection is as shown in table 2：

Table 2

From the data analysis of table 2, micropore filtering film prepared by the present invention has good anti-microbial property, and antibiotic rate Reach more than 99%.

The preparation example IV of compound fruit wine

Selection soft texture, belly prolong linea ventralis split, pulp be creamy white transparence Fructus Akebiae with maturity well, nothing Mechanical damage, non-rot grape, according to 1:1 mass ratio mixing is broken to be obtained mixing pulp；By 0.8g/L ratio Example takes pectase to add mixing pulp, digests 24h in 50 DEG C, then the total sugar content of blended fruit juice is adjusted into >=204g/ with sucrose L, pol are about 20 ° of Brix, then adjust pH to 3.0 with citric acid, obtain Mixed adjustment pulp；By 1L Mixed adjustment pulps 0.25g ratio takes dry ferment, is added in 5% glucose solution, is well mixed, is activated in 35 DEG C of water bath with thermostatic control 30min, when its aerogenesis bubble to be seen enriches, that is, obtain activated yeast；The Mixed adjustment pulp obtained is sterilized at 68 DEG C 30min, sodium hydrogensulfite is added in 0.006mg/L ratio after cooling, obtained activated yeast is then inoculated with, is issued in 18 DEG C Ferment, the residual sugar amount in a wine liquid is determined every 12h, when residual sugar amount, which continues 2d, keeps constant, terminate fermentation, use micro porous filtration Membrane filtration, the compound fruit wine clarified.

The preparation embodiment five of compound fruit wine

Selection soft texture, belly prolong linea ventralis split, pulp be creamy white transparence Fructus Akebiae with maturity well, nothing Mechanical damage, non-rot grape, according to 1:5 mass ratio mixing is broken to be obtained mixing pulp；By 0.8g/L ratio Example takes pectase to add mixing pulp, digests 24h in 50 DEG C, then the total sugar content of blended fruit juice is adjusted into >=204g/ with sucrose L, pol are about 25 ° of Brix, then adjust pH to 4.0 with citric acid, obtain Mixed adjustment pulp；By 1L Mixed adjustment pulps 0.35g ratio takes dry ferment, is added in 5% glucose solution, is well mixed, is activated in 35 DEG C of water bath with thermostatic control 30min, when its aerogenesis bubble to be seen enriches, that is, obtain activated yeast；The Mixed adjustment pulp obtained is sterilized at 68 DEG C 30min, sodium hydrogensulfite is added in 0.006mg/L ratio after cooling, obtained activated yeast is then inoculated with, is issued in 25 DEG C Ferment, the residual sugar amount in a wine liquid is determined every 12h, when residual sugar amount, which continues 2d, keeps constant, terminate fermentation, use micro porous filtration Membrane filtration, the compound fruit wine clarified.

The preparation embodiment six of compound fruit wine

Selection soft texture, belly prolong linea ventralis split, pulp be creamy white transparence Fructus Akebiae with maturity well, nothing Mechanical damage, non-rot grape, according to 1:2 mass ratio mixing is broken to be obtained mixing pulp；By 0.8g/L ratio Example takes pectase to add mixing pulp, digests 24h in 50 DEG C, then the total sugar content of blended fruit juice is adjusted into >=204g/ with sucrose L, pol are about 22 ° of Brix, then adjust pH to 3.2 with citric acid, obtain Mixed adjustment pulp；By 1L Mixed adjustment pulps 0.27g ratio takes dry ferment, is added in 5% glucose solution, is well mixed, is activated in 35 DEG C of water bath with thermostatic control 30min, when its aerogenesis bubble to be seen enriches, that is, obtain activated yeast；The Mixed adjustment pulp obtained is sterilized at 68 DEG C 30min, sodium hydrogensulfite is added in 0.006mg/L ratio after cooling, obtained activated yeast is then inoculated with, is issued in 20 DEG C Ferment, the residual sugar amount in a wine liquid is determined every 12h, when residual sugar amount, which continues 2d, keeps constant, terminate fermentation, use micro porous filtration Membrane filtration, the compound fruit wine clarified.

The preparation embodiment seven of compound fruit wine

Selection soft texture, belly prolong linea ventralis split, pulp be creamy white transparence Fructus Akebiae with maturity well, nothing Mechanical damage, non-rot grape, according to 1:3 mass ratio mixing is broken to be obtained mixing pulp；By 0.8g/L ratio Example takes pectase to add mixing pulp, digests 24h in 50 DEG C, then the total sugar content of blended fruit juice is adjusted into >=204g/ with sucrose L, pol are about 23 ° of Brix, then adjust pH to 3.5 with citric acid, obtain Mixed adjustment pulp；By 1L Mixed adjustment pulps 0.32g ratio takes dry ferment, is added in 5% glucose solution, is well mixed, is activated in 35 DEG C of water bath with thermostatic control 30min, when its aerogenesis bubble to be seen enriches, that is, obtain activated yeast；The Mixed adjustment pulp obtained is sterilized at 68 DEG C 30min, sodium hydrogensulfite is added in 0.006mg/L ratio after cooling, obtained activated yeast is then inoculated with, is issued in 22 DEG C Ferment, the residual sugar amount in a wine liquid is determined every 12h, when residual sugar amount, which continues 2d, keeps constant, terminate fermentation, use micro porous filtration Membrane filtration, the compound fruit wine clarified.

The compound fruit wine that embodiment seven is prepared in Example four, respectively to composite fruit colours of wine, aroma, mouth Taste, flavor carry out sensory test, and evaluating result is as shown in table 3：

Table 3

The compound fruit wine that embodiment seven is prepared in Example four, the main physical and chemical index of compound fruit wine is entered respectively Row detection, testing result are as shown in table 4：

Table 4

From table 3, the data analysis of table 4, Fructus Akebiae compound fruit wine prepared by the present invention has strong Fructus Akebiae, grape Aroma, the pleasing coordination of outward appearance, clear, full color, mouthfeel is soft full, sour-sweet appropriate, and wine volume property is stable.

The present invention reaches clarification, purifies the purpose of compound fruit wine, compound fruit wine by micro porous filtration membrane filtration compound fruit wine The impurity such as pulp, fiber, saccharomycete, miscellaneous bacteria and the protein of middle residual remove fully, and whole process is clear without using fining agent Clear technique is simple and easy to control, avoids influence of the fining agent improper use to wine and women-sensual pursuits, aroma and mixed degree；The Fructus Akebiae composite fruit of preparation Wine has an aroma of strong Fructus Akebiae and grape, the pleasing coordination of outward appearance, clear, full color, and mouthfeel is soft full, sour Phenomena such as comfortable to work as, wine volume property is stable, and storage cycle length is not in muddy, sedimentation and brown stain.

The above embodiments are merely illustrative of the technical solutions of the present invention and it is unrestricted, although with reference to preferred embodiment to this hair It is bright to be described in detail, it will be understood by those within the art that, technical scheme can be modified Or equivalent substitution, without departing from the objective and scope of technical solution of the present invention, it all should cover the claim in the present invention Among scope.The present invention be not described in detail technology, shape, construction part be known technology.

Claims

A kind of 1. preparation method of Fructus Akebiae compound fruit wine, it is characterised in that：Comprise the following steps:

Step 1, the pre-treatment of raw material：Selection soft texture, belly prolong that linea ventralis splits, pulp is creamy white August of transparence Melon and maturity are good, have no mechanical damage, non-rot grape, according to 1:1~1:5 mass ratio mixing is broken to be obtained Mix pulp；

Step 2, the adjustment of composition：The mixing pulp obtained in step 1 is taken to add pectase in 0.8g/L ratio, in 50 DEG C 24h is digested, then the total sugar content of blended fruit juice is adjusted to >=204g/L with sucrose, pol is about 20~25 ° of Brix, then uses lemon Lemon acid adjustment pH to 3.0~4.0, obtains Mixed adjustment pulp；

Step 3, the activation of yeast：Dry ferment is taken in 0.25~0.35g of 1L Mixed adjustment pulps ratio, is added to 5% Portugal In grape sugar juice, it is well mixed, 30min is activated in 35 DEG C of water bath with thermostatic control, when its aerogenesis bubble to be seen enriches, that is, is lived Change yeast；

Step 4, compound fruit wine are brewageed：Take the Mixed adjustment pulp obtained in step 2 to sterilize 30min at 68 DEG C, cool down Afterwards sodium hydrogensulfite, the activated yeast then obtained in inoculation step three, at 18~25 DEG C are added in 0.006mg/L ratio Fermentation, the residual sugar amount in a wine liquid is determined every 12h, when residual sugar amount, which continues 2d, keeps constant, terminate fermentation, with micropore mistake Membrane filtration, that is, the compound fruit wine clarified.
A kind of 2. preparation method of Fructus Akebiae compound fruit wine according to claim 1, it is characterised in that：In the step 1 The mass ratio of Fructus Akebiae and grape is 1:2~1:3.
A kind of 3. preparation method of Fructus Akebiae compound fruit wine according to claim 2, it is characterised in that：In the step 2 The pH of Mixed adjustment pulp is 3.2~3.5.
A kind of 4. preparation method of Fructus Akebiae compound fruit wine according to claim 3, it is characterised in that：In the step 4 The fermentation temperature of Mixed adjustment pulp is 18~20 DEG C.
A kind of 5. preparation method of Fructus Akebiae compound fruit wine according to claim 4, it is characterised in that：Described in step 4 Micropore filtering film aperture be 0.18~0.22 μm, its preparation method is as follows：

1) preparation of hectorite/cetyl trimethylammonium bromide/Chitosan powder：Hectorite is taken to be configured to deionized water 2wt% hectorite colloidal solution, the cetyl trimethylammonium bromide of 0.4~0.6 times of quality of hectorite is added, in 80 DEG C of water The lower stirring reaction 6h of bath, adds the chitosan of 2~3 times of quality of hectorite, continues after reacting 6h, stand 2h, be centrifuged off Supernatant, precipitation deionized water rinsing to neutrality, is then dried, and grinding, obtains hectorite/cetyl trimethyl bromination Ammonium/Chitosan powder；

2) preparation of casting solution：Polypropylene is taken in reactor, adds the adipic acid of 0.2~0.4 times of quality of polypropylene, Yi Jiju The soybean oil of 2.1~2.3 times of quality of propylene, in 150~160 DEG C, stirred under nitrogen atmosphere 3h, homogeneous phase solution is obtained, is then dropped Temperature is to 70~80 DEG C, in the hectorite/cetyl trimethyl bromine obtained in the ratio addition 1) of homogeneous phase solution quality 5~8% Change ammonium/Chitosan powder, after being well mixed under 1500~2000r/min rotating speed, standing and defoaming, treat that casting solution deaeration is complete Entirely, casting solution is obtained；

3) preparation of micropore filtering film：Casting solution is taken to be transferred quickly to be film-made in knifing machine, wherein quenching temperature is 35~45 DEG C, Scraper temperature is 200 DEG C, and obtained film then is extracted into 24h in 25~35 DEG C of n-hexane, obtains micropore filtering film.
A kind of 6. preparation method of Fructus Akebiae compound fruit wine according to claim 5, it is characterised in that：The micro porous filtration Film also have passed through hydrophilic modification, and hydrophilic modification method is as follows：

By micropore filtering film acetone extraction 12h, be dried in vacuo 6h, be then placed in ozone reactor carry out ozone processing 30~ 60min, vacuum outgas 30min after the completion of ozone processing, then 10min is soaked with ethanol, it is then immersed in iron ammonium sulfate and propylene In the mixed aqueous solution of acid monomers, letting nitrogen in and deoxidizing, 1~2h is reacted at 45 DEG C, micropore filtering film is then taken out and is extracted with methanol 12h, then cleaned for several times with water, 6h is dried in vacuo, that is, obtains modified micropore filter membrane.
A kind of 7. preparation method of Fructus Akebiae compound fruit wine according to claim 5, it is characterised in that：Described in step 2) Standing and defoaming carried out at 70~80 DEG C of temperature, 0.090~0.095MPa of vacuum vacuum condition.
A kind of 8. preparation method of Fructus Akebiae compound fruit wine according to claim 6, it is characterised in that：The vacuum drying Condition be：40~45 DEG C of temperature, 0.09~0.10MPa of vacuum.
A kind of 9. preparation method of Fructus Akebiae compound fruit wine according to claim 6, it is characterised in that：The ferrous sulfate In the mixed aqueous solution of ammonium and acrylic monomers, the volume ratio of acrylic monomers and deionized water is 1:3, iron ammonium sulfate and third The mol ratio of acrylic monomer is 1.2:1.