CN107522566A - The organic fungi-manure of antibiotic in a kind of soil that can degrade - Google Patents

The organic fungi-manure of antibiotic in a kind of soil that can degrade Download PDF

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Publication number
CN107522566A
CN107522566A CN201710911514.6A CN201710911514A CN107522566A CN 107522566 A CN107522566 A CN 107522566A CN 201710911514 A CN201710911514 A CN 201710911514A CN 107522566 A CN107522566 A CN 107522566A
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China
Prior art keywords
antibiotic
microbial inoculum
soil
parts
degrade
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CN201710911514.6A
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Chinese (zh)
Inventor
文贻荣
黄生博
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Department Of Agriculture Co Ltd In Hunan Province
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Department Of Agriculture Co Ltd In Hunan Province
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Priority to CN201710911514.6A priority Critical patent/CN107522566A/en
Publication of CN107522566A publication Critical patent/CN107522566A/en
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00Other organic fertilisers
    • C05F11/08Organic fertilisers containing added bacterial cultures, mycelia or the like
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners

Abstract

The organic fungi-manure of antibiotic, is related to technical field of biological organic fertilizer, is mixed by the raw material of following parts by weight in a kind of soil that can degrade disclosed by the invention:Bacillus subtilis J5P2 13, bacillus 13, nitrogen-fixing bacteria 13, pseudomonad J2 24, nitragin 13, phosphate solubilizing bacteria 13, actinomyces 5406 24, potassium solubilizing bacteria 13, saccharomycete 13, purple soil 100 150, organic fertilizer 250 300.Have the characteristics that to degrade antibiotic, and can culture fertility in soil, is repaired available for by antibiotic contaminated lands such as tetracycline, penicillin, sulphadiazine class, quinolones.

Description

The organic fungi-manure of antibiotic in a kind of soil that can degrade
Technical field
The present invention relates to technical field of biological organic fertilizer, the organic fungi-manure of antibiotic in particularly a kind of soil that can degrade.
Background technology
With a large amount of productions and application of antibiotic (Tetracyclines, penicillins, sulphadiazine class, quinolones etc.), Pollution problem also becomes increasingly severe, and its abuse can cause antibiotic to be prevalent in environment (especially soil environment In), the serious pollution of soil environment is caused, and then jeopardize human health.China's antibiotic pollution source mainly has three sides Face:
First, medical antibiotic pollution.The pollution of medical antibiotic be considered as current antibiotic pollution main path it One.
Second, feeding antibiotic pollutes.Because antibiotic cost is low, since the 1950s, poultry has been widely used in In fowl cultivation, in poultry industry and culture fishery, prevention from suffering from the diseases agent and growth promoter as animal are fast to improve growth Rate obtains larger economic benefit.At present, substantial amounts of feeding antibiotic uses as feed addictive, referred to as antimicrobial growth Accelerator (antimicrobial growthpromoter, AGP).When in use, animal has no clear and definite illness to AGP in itself, Purpose is also not intended to treat certain disease, but to promote the growth of animal, improve Production of Livestock and Poultry efficiency.
Third, farm antibiotics pollutes.Utilize antibiotic controlling plant diseases, very early someone's research.Most early in agricultural The antibiotic of upper large-area applications will push away streptomysin, terramycin and its mixed liquor " agrimycin ".They to apple, pears, English walnut, Bacterial disease of the plants such as citrus, tobacco, vegetables, beans etc. has special efficacy, to a small number of fungal diseases such as downy mildew, phytophthora etc. Also there is preventive and therapeutic effect.Blasticidin S is first and applies and obtain very successful farm antibiotics over a large area, and it was once Great function has been played in Japanese rice blast within pandemic 1963.
The use for reducing antibiotic is the fundamental solution for solving antibiotic pollution, and the removal of antibiotic residue is also to reduce The effective way of antibiotic pollution.The removing method that antibiotic pollutes in soil has many, including physics, chemical and life Thing.Traditional physical method (with soil moved in improve the original, Soil leaching, applying based on stabilizer) and chemical method (including aoxidize, reduce And hydrolysis) cost height, it is possible to create new pollutant, and act on relatively slow.Using biology or biological product come pollution degradation The biological renovation method of thing has the advantages that nontoxic, noresidue, non-secondary pollution, is the one of elimination and degraded antibiotic residue The method of kind safely, effectively, cheap.At present, external many experts and scholars are directed to studying the biodegradation of antibiotic in soil Also there are antibiotic degradation technique in many soil and the report of the research in terms of degrading microorganism screening in technology, the country, reported The soil that can degrade in the microorganism of antibiotic have bacterium, fungi, actinomyces and algae etc..For example, Wang Liqun etc. is from lactams Separation screening there is efficient degradation to act on such antibiotic and has stronger tolerance to 4 plants in ring class antibiotic production wastewater The effect bacterial strain of ability, they are respectively acinetobacter, pseudomonas, Escherichia and bacillus;Wang Yongjie Deng from sludge separation screening to 1 plant in a manner of Co metabolism can wide spectrum degraded antibiotic bacillus, be initially identified as lichens Bacillus (B.licheniformis).
Disclosed " the mycorrhizal fungi microbial inoculum and its residual degrading of Chinese patent (number of patent application 201410348058.5) Stay the application in terms of tetracycline antibiotics ", the microbial inoculum includes culture matrix and the clump branch bacterium being mixed in the culture matrix The spore of mycorrhiza fungi and/or mycelia.Application of the mycorrhizal fungi microbial inoculum in terms of degraded remains tetracycline antibiotics is specific To be inoculated with above-mentioned mycorrhizal fungi microbial inoculum in tetracycline antibiotics contaminated soil, then host is sowed in the soil again Plant, system is repaired so as to build tetracycline antibiotics contaminated soil.Host plant grows 90-120 days to be surveyed after harvest Determine the concentration of tetracycline antibiotics in soil, its degradation rate is up to 20-60%.
Another Chinese patent (number of patent application 201610807117.X) disclosed " one kind degraded antibiotic and agricultural chemicals The complex micro organism fungicide of residual and its preparation and application ", the complex micro organism fungicide of the invention account for compound bacteria by each live body bacterium The percentage by weight of powder total viable count is configured to more thalline composite bacterium powders, wherein, bacillus subtilis 10-15%, aspergillus niger 10-15%, colloid bacillus cereus 10-15%, enterococcus faecalis 10-15%, bacillus licheniformis 8-12%, bacillus megaterium 8-12%, Pseudomonas fluorescens 8-12%, Lactobacillus plantarum 5-8%, bacillus polymyxa 5-8%, streptococcus thermophilus 6- 8%.The complex micro organism fungicide of the invention has that degraded antibiotic and residues of pesticides, organic matter fermentation are decomposed, multi-Functional Fertilizers And the effect of repairing environment, applied to agricultural and ecological environment, can solve the secondary pollution for cultivating antibiotic residue in excreta And the problem that reclaiming organic waste utilizes, the biodegradation of antibiotic and residues of pesticides in soil is realized, to Agro-ecology Environment remediation and guarantee human health have important value and realistic meaning.
Also " biological organic fertilizer compound probiotic disclosed in Chinese patent (number of patent application 201110131561.1) Agent and preparation method thereof ", step are that 14 probiotics, pseudomonad, purple variant streptomycete, rhizobium, brown is spherical solid Nitrogen bacterium, bacillus subtilis, bacillus amyloliquefaciens, Bacillus cercus, bacillus licheniformis, Bacillus weihenstephanensis, jelly After sample bacillus, bacillus thuringiensis, saccharomyces rouxii, the mutation of streptomyces griseus rust, streptomyces microflavus activation culture, press According to 1%-5% ratio combined inoculation into 500mL liquid seed culture mediums, culture is then enlarged, finally by strain It is transported in 500L fermentation tanks, 28-30 DEG C of temperature, 200 revs/min of rotating speed, ferments 3-5 days, liquid compound probiotic can be obtained Agent.
The content of the invention
It is an object of the invention to provide the fertilizer of antibiotic, and can culture fertility in a kind of soil that can degrade.
To achieve the above object, the technical measures that the present invention is taken are antibiotic in a kind of soil that can degrade of invention Organic fungi-manure, mixed by the raw material of following parts by weight:
Described nitragin includes soybean rhizobium inoculant, peanut rhizobium inoculant and herbage nitragin, and each component is matched somebody with somebody Than for etc. parts by weight or other ratios.
Described herbage nitragin includes sweet potato knurl microbial inoculum, Chinese milk vetch knurl microbial inoculum, sesbania knurl microbial inoculum, sainfoin knurl bacterium Agent, red clover knurl microbial inoculum, butch clover knurl microbial inoculum, khuskhus knurl microbial inoculum, white popinac knurl microbial inoculum, big winged bean knurl microbial inoculum, hundred arteries and veins One or more in nitragin, Red kidney bean knurl microbial inoculum, alyce clover knurl microbial inoculum, beggarweed knurl microbial inoculum, when to be two or more When, it parts by weight or other ratios such as the ratio of each component is.
Its technical scheme one optimized is mixed by the raw material of following parts by weight:
Its technical scheme two optimized is mixed by the raw material of following parts by weight:
Its technical scheme three optimized is mixed by the raw material of following parts by weight:
Its preparation method is:First by bacillus subtilis J5P2, bacillus, nitrogen-fixing bacteria, pseudomonad J2, rhizobium Agent, phosphate solubilizing bacteria, actinomyces 5406, potassium solubilizing bacteria, saccharomycete are well mixed, and are then well mixed with purple soil, then with organic fertilizer (for commercially available organic fertilizer) is well mixed, the organic fungi-manure of antibiotic in the soil that can as degrade.
The organic fungi-manure of antibiotic is used for the degraded of soil antibiotic residue, its advantage in the soil that can degrade of the present invention It is:
1st, strong stress resistance and degradation enzyme system enriches, degraded substrate spectrum is wide, and it can not only degrade using terramycin as representative Telracycline family, the penicillin family antibiotic using penicillin as representative, moreover it is possible to the antibiosis such as sulphadiazine class, quinolones of degrading Element.
2nd, can culture fertility.First, nutrient of contained organic fertilizer itself is sufficient;Second, a variety of microbial inoculums being capable of fixed nitrogen, phosphorus decomposing The addition of potassium decomposing, especially nitragin, root nodule environment can be formed, strengthen nitrogen fixation effect;Purple soil used can buffer Antagonism between each microbial inoculum, form the symbiotic environment of close friend.
3rd, it is good to antibiotic contaminated soil repairing effect, can poisonous and hazardous antibiotic under microbial action, The structure of antibiotic residues is set to change, so as to cause its chemical and physical features to change, i.e., by by antibiotic Micromolecular compound is degraded to from macromolecular compound, is finally decomposed into H2O and CO2, realize the innoxious of environmental pollution The process of processing, its transformation efficiency is high, and environment protecting is obvious.
Embodiment
With reference to embodiments, the present invention is further illustrated.Following explanation be in a manner of enumerating, but this The protection domain of invention is not limited thereto.
Embodiment 1:
The organic fungi-manure of antibiotic, its preparation process are as follows in the soil that can degrade of the present embodiment:
(1), stock up:Take 1 part of bacillus subtilis J5P2,1 part of bacillus, 1 part of nitrogen-fixing bacteria, vacation respectively by weight 4 parts of monad J2,1 part of nitragin, 3 parts of phosphate solubilizing bacteria, 4 parts of actinomyces 5406,3 parts of potassium solubilizing bacteria, 3 parts of saccharomycete, purple soil 100 parts, 300 parts of organic fertilizer, it is standby;
(2), mix:First by bacillus subtilis J5P2, bacillus, nitrogen-fixing bacteria, pseudomonad J2, nitragin, solution Phosphorus bacterium, actinomyces 5406, potassium solubilizing bacteria, saccharomycete are well mixed, and are then well mixed with purple soil, then with organic fertilizer (to be commercially available Organic fertilizer) well mixed, the organic fungi-manure of antibiotic in the soil that can as degrade.
Embodiment 2:
The organic fungi-manure of antibiotic, its preparation process are as follows in the soil that can degrade of the present embodiment:
(1), stock up:Take 3 parts of bacillus subtilis J5P2,3 parts of bacillus, 3 parts of nitrogen-fixing bacteria, vacation respectively by weight 2 parts of monad J2,3 parts of nitragin, 1 part of phosphate solubilizing bacteria, 2 parts of actinomyces 5406,1 part of potassium solubilizing bacteria, 1 part of saccharomycete, purple soil 150 parts, 250 parts of organic fertilizer, it is standby;
(2), mix:First by bacillus subtilis J5P2, bacillus, nitrogen-fixing bacteria, pseudomonad J2, nitragin, solution Phosphorus bacterium, actinomyces 5406, potassium solubilizing bacteria, saccharomycete are well mixed, and are then well mixed with purple soil, then with organic fertilizer (to be commercially available Organic fertilizer) well mixed, the organic fungi-manure of antibiotic in the soil that can as degrade.
Embodiment 3:
The organic fungi-manure of antibiotic, its preparation process are as follows in the soil that can degrade of the present embodiment:
(1), stock up:Take 2 parts of bacillus subtilis J5P2,2 parts of bacillus, 2 parts of nitrogen-fixing bacteria, vacation respectively by weight 3 parts of monad J2,2 parts of nitragin, 2 parts of phosphate solubilizing bacteria, 3 parts of actinomyces 5406,2 parts of potassium solubilizing bacteria, 2 parts of saccharomycete, purple soil 120 parts, 280 parts of organic fertilizer, it is standby;
(2), mix:First by bacillus subtilis J5P2, bacillus, nitrogen-fixing bacteria, pseudomonad J2, nitragin, solution Phosphorus bacterium, actinomyces 5406, potassium solubilizing bacteria, saccharomycete are well mixed, and are then well mixed with purple soil, then with organic fertilizer (to be commercially available Organic fertilizer) well mixed, the organic fungi-manure of antibiotic in the soil that can as degrade.
The organic fungi-manure of antibiotic, the once multiple repetition test in field, its experimental conditions in the soil that can degrade of the present invention It is as follows:
Field test:Traffic convenience is chosen, is easy to observe and manages, representational field, it is desirable to which field is upright, field (this experiment selects in Hunan the rice terrace that face is smooth, fertility is uniform, irrigation and drainage are convenient, plantation level is suitable with the local level of production In Dongan County of Yongzhou City city of province).
This experiment is divided into two groups:
(1), control group:Conventional fertilizer application (commercially available organic fertilizer)+repeatedly spraying sulphadiazine class antibiotic, (its concentration is Field 500mg/L).
(2), treatment group:The organic fungi-manure (embodiment 3)+repeatedly spray sulphur of antibiotic in the soil that can degrade of the present invention The field of amic metadiazine class antibiotic (its concentration is 500mg/L), experiment set 3 repetitions, random alignment, and plot area is not less than 60 square metres.
Dose is 50kg/666 ㎡, is sampled respectively after using 0,5,10,20,25,30 day, and serpentine is pressed per cell Method takes soil sample censorship, the residual quantity change of detection soil sample sulphadiazine class.Sulphadiazine class residual quantity result is as shown in table 1, Sulphadiazine class antibiotic has been degraded substantially after the organic fungi-manure 30 days of antibiotic in the soil that can degrade using the present invention Entirely.
The sulphadiazine class residual quantity (mg/Kg) of table 1
Above-mentioned experiment, continuously in different places in 3 years, the field of different area, such as 60 square metres, 80 square metres, 120 square metres are waited experiment 18 times, achieve identical and close result.In test, ill effect is not found, this table The organic fungi-manure of antibiotic has the function that antibiotic residue of degrading well in the bright soil that can degrade of the invention, uses peace Entirely, it is stable and reliable for performance, the toxic side effect of antibiotic can be effectively reduced, is advantageous to high yield good harvest and the body of people of agricultural Health.
The organic fungi-manure of antibiotic in the soil that can degrade of the present invention, available for by tetracycline, penicillin, sulphadiazine The reparation of the antibiotic contaminated land such as class, quinolones.

Claims (5)

  1. A kind of 1. organic fungi-manure of antibiotic in soil that can degrade, it is characterised in that be by following parts by weight raw material mixing and Into:
    Described nitragin includes soybean rhizobium inoculant, peanut rhizobium inoculant and herbage nitragin, the ratio of each component is Etc. parts by weight or other ratios.
  2. 2. the organic fungi-manure of antibiotic in the soil that can degrade according to claim 1, it is characterised in that:Described herbage root nodule Microbial inoculum includes sweet potato knurl microbial inoculum, Chinese milk vetch knurl microbial inoculum, sesbania knurl microbial inoculum, sainfoin knurl microbial inoculum, red clover knurl microbial inoculum, Trifolium repense Careless knurl microbial inoculum, khuskhus knurl microbial inoculum, white popinac knurl microbial inoculum, big winged bean knurl microbial inoculum, crowtoe knurl microbial inoculum, Red kidney bean knurl microbial inoculum, chain One or more in pod beans knurl microbial inoculum, beggarweed knurl microbial inoculum, when for it is two or more when, the ratio of each component is etc. parts by weight or Other ratios.
  3. 3. the organic fungi-manure of antibiotic in the soil that can degrade according to claim 2, it is characterised in that be by following parts by weight Raw material mix:
  4. 4. the organic fungi-manure of antibiotic in the soil that can degrade according to claim 2, it is characterised in that be by following parts by weight Raw material mix:
  5. 5. the organic fungi-manure of antibiotic in the soil that can degrade according to claim 2, it is characterised in that be by following parts by weight Raw material mix:
CN201710911514.6A 2017-09-29 2017-09-29 The organic fungi-manure of antibiotic in a kind of soil that can degrade Pending CN107522566A (en)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103834565A (en) * 2012-11-27 2014-06-04 南阳奇伟微生态基因科技开发有限公司 Micro-ecological flora of crop root system probiotics and application thereof
CN106635933A (en) * 2017-02-28 2017-05-10 湖南省中科农业有限公司 Mixed bacterial agent capable of degrading antibiotics in soil and preparation method thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103834565A (en) * 2012-11-27 2014-06-04 南阳奇伟微生态基因科技开发有限公司 Micro-ecological flora of crop root system probiotics and application thereof
CN106635933A (en) * 2017-02-28 2017-05-10 湖南省中科农业有限公司 Mixed bacterial agent capable of degrading antibiotics in soil and preparation method thereof

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