CN107466912B - Cuttlefish and cuttlebone-based oxytocin embedding method - Google Patents
Cuttlefish and cuttlebone-based oxytocin embedding method Download PDFInfo
- Publication number
- CN107466912B CN107466912B CN201710597599.5A CN201710597599A CN107466912B CN 107466912 B CN107466912 B CN 107466912B CN 201710597599 A CN201710597599 A CN 201710597599A CN 107466912 B CN107466912 B CN 107466912B
- Authority
- CN
- China
- Prior art keywords
- cuttlefish
- parts
- cuttlebone
- stirring
- hormone
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000238371 Sepiidae Species 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title claims abstract description 25
- XNOPRXBHLZRZKH-UHFFFAOYSA-N Oxytocin Natural products N1C(=O)C(N)CSSCC(C(=O)N2C(CCC2)C(=O)NC(CC(C)C)C(=O)NCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(CCC(N)=O)NC(=O)C(C(C)CC)NC(=O)C1CC1=CC=C(O)C=C1 XNOPRXBHLZRZKH-UHFFFAOYSA-N 0.000 title claims abstract description 14
- 101800000989 Oxytocin Proteins 0.000 title claims abstract description 14
- 102100031951 Oxytocin-neurophysin 1 Human genes 0.000 title claims abstract description 14
- XNOPRXBHLZRZKH-DSZYJQQASA-N oxytocin Chemical compound C([C@H]1C(=O)N[C@H](C(N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CSSC[C@H](N)C(=O)N1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(C)C)C(=O)NCC(N)=O)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 XNOPRXBHLZRZKH-DSZYJQQASA-N 0.000 title claims abstract description 14
- 229960001723 oxytocin Drugs 0.000 title claims abstract description 14
- 229940088597 hormone Drugs 0.000 claims abstract description 28
- 239000005556 hormone Substances 0.000 claims abstract description 28
- 230000003444 anaesthetic effect Effects 0.000 claims abstract description 22
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 22
- 206010002091 Anaesthesia Diseases 0.000 claims abstract description 20
- 230000037005 anaesthesia Effects 0.000 claims abstract description 20
- HRLIOXLXPOHXTA-NSHDSACASA-N dexmedetomidine Chemical compound C1([C@@H](C)C=2C(=C(C)C=CC=2)C)=CN=C[N]1 HRLIOXLXPOHXTA-NSHDSACASA-N 0.000 claims abstract description 11
- 229960004253 dexmedetomidine Drugs 0.000 claims abstract description 11
- 238000013268 sustained release Methods 0.000 claims abstract description 10
- 239000012730 sustained-release form Substances 0.000 claims abstract description 10
- NMJREATYWWNIKX-UHFFFAOYSA-N GnRH Chemical compound C1CCC(C(=O)NCC(N)=O)N1C(=O)C(CC(C)C)NC(=O)C(CC=1C2=CC=CC=C2NC=1)NC(=O)CNC(=O)C(NC(=O)C(CO)NC(=O)C(CC=1C2=CC=CC=C2NC=1)NC(=O)C(CC=1NC=NC=1)NC(=O)C1NC(=O)CC1)CC1=CC=C(O)C=C1 NMJREATYWWNIKX-UHFFFAOYSA-N 0.000 claims abstract description 9
- 101000857870 Squalus acanthias Gonadoliberin Proteins 0.000 claims abstract description 9
- 229920001661 Chitosan Polymers 0.000 claims abstract description 8
- 150000003242 quaternary ammonium salts Chemical class 0.000 claims abstract description 8
- 238000003756 stirring Methods 0.000 claims description 25
- 238000002360 preparation method Methods 0.000 claims description 10
- CUHVIMMYOGQXCV-LLVKDONJSA-N levomedetomidine Chemical compound C1([C@H](C)C=2C(=C(C)C=CC=2)C)=CNC=N1 CUHVIMMYOGQXCV-LLVKDONJSA-N 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- CUHVIMMYOGQXCV-UHFFFAOYSA-N medetomidine Chemical compound C=1C=CC(C)=C(C)C=1C(C)C1=CNC=N1 CUHVIMMYOGQXCV-UHFFFAOYSA-N 0.000 claims description 6
- 229960002140 medetomidine Drugs 0.000 claims description 6
- 239000013535 sea water Substances 0.000 claims description 6
- 239000004156 Azodicarbonamide Substances 0.000 claims description 5
- XOZUGNYVDXMRKW-AATRIKPKSA-N azodicarbonamide Chemical compound NC(=O)\N=N\C(N)=O XOZUGNYVDXMRKW-AATRIKPKSA-N 0.000 claims description 5
- 235000019399 azodicarbonamide Nutrition 0.000 claims description 5
- 239000012153 distilled water Substances 0.000 claims description 5
- 238000004108 freeze drying Methods 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- 235000019832 sodium triphosphate Nutrition 0.000 claims description 5
- 239000008247 solid mixture Substances 0.000 claims description 5
- BRUQQQPBMZOVGD-XFKAJCMBSA-N Oxycodone Chemical compound O=C([C@@H]1O2)CC[C@@]3(O)[C@H]4CC5=CC=C(OC)C2=C5[C@@]13CCN4C BRUQQQPBMZOVGD-XFKAJCMBSA-N 0.000 claims description 3
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- 238000005553 drilling Methods 0.000 claims description 3
- 229960002085 oxycodone Drugs 0.000 claims description 3
- 239000004615 ingredient Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 8
- 230000000202 analgesic effect Effects 0.000 abstract description 6
- 238000006243 chemical reaction Methods 0.000 abstract description 5
- 230000000638 stimulation Effects 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract 1
- VPNGEIHDPSLNMU-RFVHGSKJSA-N levomedetomidine hydrochloride Chemical compound Cl.C1([C@H](C)C=2C(=C(C)C=CC=2)C)=CNC=N1 VPNGEIHDPSLNMU-RFVHGSKJSA-N 0.000 abstract 1
- 238000012423 maintenance Methods 0.000 description 8
- 238000009395 breeding Methods 0.000 description 7
- 230000001488 breeding effect Effects 0.000 description 7
- 241000238366 Cephalopoda Species 0.000 description 4
- 230000006698 induction Effects 0.000 description 4
- 210000003205 muscle Anatomy 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 238000002695 general anesthesia Methods 0.000 description 2
- 102000012673 Follicle Stimulating Hormone Human genes 0.000 description 1
- 108010079345 Follicle Stimulating Hormone Proteins 0.000 description 1
- 102000009151 Luteinizing Hormone Human genes 0.000 description 1
- 108010073521 Luteinizing Hormone Proteins 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 229940028334 follicle stimulating hormone Drugs 0.000 description 1
- 229940040129 luteinizing hormone Drugs 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000005070 ripening Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
- A61K38/08—Peptides having 5 to 11 amino acids
- A61K38/09—Luteinising hormone-releasing hormone [LHRH], i.e. Gonadotropin-releasing hormone [GnRH]; Related peptides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/16—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing nitrogen, e.g. nitro-, nitroso-, azo-compounds, nitriles, cyanates
- A61K47/18—Amines; Amides; Ureas; Quaternary ammonium compounds; Amino acids; Oligopeptides having up to five amino acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0002—Galenical forms characterised by the drug release technique; Application systems commanded by energy
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Epidemiology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Environmental Sciences (AREA)
- Endocrinology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Marine Sciences & Fisheries (AREA)
- Reproductive Health (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Immunology (AREA)
- Inorganic Chemistry (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses an oxytocin embedding method based on cuttlebone, which comprises the steps of firstly placing cuttlefish in an anesthetic for anesthesia, and then implanting a hormone slow release agent into the cuttlebone. The beneficial effects are that: the implanted oxytocin is positioned in the calcareous cuttlebone of the cuttlefish, has small stimulation effect on the cuttlefish, and is easy to release the hormone into the body to play a role due to the loose and porous structure of the cuttlebone; a certain amount of levomedetomidine is added into the dexmedetomidine, so that the prepared anesthetic has long anesthesia maintaining time and a certain analgesic effect, the stress reaction of the cuttlefish to the embedding process is greatly reduced, and the ink jet is avoided; the prepared GnRH sustained-release agent has good biocompatibility, long release time and uniform release speed by taking the chitosan quaternary ammonium salt as a carrier.
Description
Technical Field
The invention relates to the technical field of aquaculture, in particular to an oxytocin embedding method based on cuttlebone.
Background
The cuttlefish is one of four fisheries in China, has strong adaptability to temperature, is widely distributed in China along the sea, and is a traditional fishery type with high economic value. Due to over-fishing and ecological deterioration, the decline phenomenon appeared since the 70 s of the 20 th century. In order to alleviate the dilemma, artificial breeding of cuttlefish is an effective way to solve the above problems. In recent years, breeding and breeding of cuttlefish are well developed in China, but artificial breeding of cuttlefish still depends on natural breeding and spawning, and artificial induced spawning is urgently needed to increase the number of cuttlefish.
In the prior art, for example, Chinese patent with application publication No. CN 106719178A discloses an artificial ripening method of cephalopods, oxytocin is implanted into orbital sinus of cephalopods to slowly release the hormone and promote the propagation of the cephalopods, but the preparation process of the hormone slow release agent in the method is complex, and the stress reaction of the cephalopods in the anesthesia process is large.
Disclosure of Invention
The invention aims to provide an oxytocin embedding method based on cuttlebone of cuttlefish, which can enable cuttlefish to mature in advance and improve the breeding capability of the cuttlefish.
Aiming at the problems mentioned in the background technology, the invention adopts the technical scheme that: an oxytocin embedding method based on cuttlebone includes anesthesia and embedding. The method is reasonable, the operation is simple, the cuttlefish does not generate stress to the embedding, the embedding success rate is high, and the economic benefit of the invention is improved.
Preferably, the preparation method of the anesthetic comprises the following steps: taking 2-4 parts of medetomidine and 1-2 parts of oxycodone, adding 9-11 parts of deionized water, stirring for 10-14min, adding 60-65 parts of seawater, and stirring for 15-20min to obtain the anesthetic. Putting cuttlefish in the prepared anesthetic for 8-10 min. The prepared anesthetic is rapid and stable in induction and long in anesthesia maintenance time. The ink jet behavior of the cuttlefish has great influence on the embedding process, which may cause the failure of hormone embedding, and the cuttlefish is anesthetized, so that the stress reaction of the cuttlefish to the embedding process is greatly reduced, and the ink jet is avoided.
Preferably, the mass ratio of dexmedetomidine to levomedetomidine in the anesthetic ingredient medetomidine is 97/3-94/6. The pure dexmedetomidine can quickly deepen general anesthesia so that the cuttlefish can quickly enter an anesthesia induction period, but the anesthesia maintenance time is short, the analgesic effect is poor, and if the dosage is increased, a certain side effect is generated on the organism. The anesthetic compounded by the dexmedetomidine and the levomedetomidine has long anesthesia maintenance time and a certain analgesic effect, and provides good conditions for embedding hormone.
Preferably, during the embedding process, the muscle mantle of cuttlefish is cut open, the exposed cuttlebone is drilled, the hormone slow release agent is added, and the cuttlefish is transferred into fresh seawater after 1 min. Cuttlebone is calcareous endoskeleton, and the implanted sustained-release agent has small stimulation effect on cuttlefish; meanwhile, as the cuttlebone has a loose and porous structure, the hormone is easy to release into the body to play a role.
Preferably, the preparation method of the hormone slow release agent used in the embedding process comprises the following steps: adding 10-15 parts of chitosan quaternary ammonium salt into 900-1000 parts of distilled water, uniformly stirring, adding 0.1-0.2 part of GnRH powder, stirring for 10-15min, then adding 5-7 parts of azodicarbonamide, stirring for 4-6min, then adding 2-3 parts of sodium tripolyphosphate, stirring for 30-35min, ultracentrifuging the liquid, and freeze-drying the obtained solid mixture to obtain the hormone slow-release agent. GnRH is a decapeptide hormone responsible for secretion of follicle stimulating hormone and luteinizing hormone, and can promote the breeding of cuttlefish. The prepared GnRH sustained-release agent has good biocompatibility by taking the chitosan quaternary ammonium salt as a carrier, the slow-release time of hormone is prolonged by adding the azodicarbonamide, the release speed is uniform, more complete release can be realized, and the preparation method is simple and effective.
Compared with the prior art, the invention has the advantages that: the implanted oxytocin is positioned in the calcareous cuttlebone of the cuttlefish, has small stimulation effect on the cuttlefish, and is easy to release the hormone into the body to play a role due to the loose and porous structure of the cuttlebone; a certain amount of levomedetomidine is added into the dexmedetomidine, so that the prepared anesthetic has long anesthesia maintaining time and a certain analgesic effect, the stress reaction of the cuttlefish to the embedding process is greatly reduced, and the ink jet is avoided; the prepared GnRH sustained-release agent has good biocompatibility, long release time and uniform release speed by taking the chitosan quaternary ammonium salt as a carrier.
Drawings
Figure 1 is a graph of the effect of the ratio of dexmedetomidine to levomedetomidine on the time of anesthesia in the anesthetic of the present invention.
Detailed Description
The invention is further illustrated by the following examples:
example 1:
an oxytocin embedding method based on cuttlebone comprises the following steps: firstly, anesthesia is carried out: scooping out the cuttlefish by using a brail net, placing the cuttlefish in an anesthetic container for 10min, and observing the anesthetic condition of the cuttlefish at any time; then embedding: cutting off the muscle mantle of cuttlefish with a sterilized knife, drilling hole with twist drill on the exposed cuttlebone, adding hormone slow-release agent, and transferring cuttlefish into fresh seawater after 1 min. The preparation method of the hormone sustained release agent comprises the following steps: putting 10 parts of chitosan quaternary ammonium salt into a container, adding 900 parts of distilled water, stirring for 10min, adding 0.1 part of GnRH powder, stirring for 10min, then adding 5 parts of azodicarbonamide, stirring for 4min, then adding 2 parts of sodium tripolyphosphate, stirring for 30min, setting the relative centrifugal force of a centrifugal machine to be 2000g, centrifuging the liquid at 4 ℃ for 35min, and freeze-drying the obtained solid mixture to obtain the hormone slow-release agent. Cuttlebone is calcareous endoskeleton, and the implanted sustained-release agent has small stimulation effect on cuttlefish; meanwhile, as the cuttlebone has a loose and porous structure, the hormone is easy to release into the body to play a role.
Example 2:
an oxytocin embedding method based on cuttlebone comprises the following steps:
1) anesthesia: putting cuttlefish in anesthetic for 9 min. The prepared anesthetic is rapid and stable in induction and long in anesthesia maintenance time. (ii) a
2) Embedding: cutting muscle mantle of cuttlefish, drilling hole on exposed cuttlebone, adding hormone slow release agent, and transferring cuttlefish into fresh seawater after 1 min. The preparation method of the hormone sustained release agent comprises the following steps: adding 15 parts of chitosan quaternary ammonium salt into 1000 parts of distilled water, uniformly stirring, adding 0.2 part of GnRH powder, stirring for 15min, adding 7 parts of azodicarbonamide, stirring for 6min, adding 3 parts of sodium tripolyphosphate, stirring for 35min, ultracentrifuging the liquid, and freeze-drying the obtained solid mixture to obtain the hormone slow-release agent. Cuttlebone is calcareous endoskeleton, and the implanted sustained-release agent has small stimulation effect on cuttlefish; meanwhile, as the cuttlebone has a loose and porous structure, the hormone is easy to release into the body to play a role.
The ink jet behavior of the cuttlefish has great influence on the embedding process, which may cause the failure of hormone embedding, and the cuttlefish is anesthetized, so that the stress reaction of the cuttlefish to the embedding process is greatly reduced, and the ink jet can be avoided. The pure dexmedetomidine can quickly deepen general anesthesia so that the cuttlefish can quickly enter an anesthesia induction period, but the anesthesia maintenance time is short, the analgesic effect is poor, and if the dosage is increased, a certain side effect is generated on the organism. The anesthetic compounded by the dexmedetomidine and the levomedetomidine has long anesthesia maintenance time and a certain analgesic effect, and provides good conditions for embedding hormone.
The preparation method of the anesthetic comprises the following steps: taking 3 parts of medetomidine and 1 part of oxycodone, adding 10 parts of deionized water, stirring for 12min, adding 62 parts of seawater, and stirring for 15min to obtain the anesthetic. The ratio of dexmedetomidine to levomedetomidine in the medetomidine components is 100/0,99/1,98/2,97/3,96/4,95/5,94/6,93/7,92/8,91/9, 90/10. The prepared anesthetic is used for the anesthesia of cuttlefish, the anesthesia maintenance time is used as an evaluation index, the result is shown in figure 1, the obtained experimental data is in nonlinear motion, and the anesthesia maintenance time is longer when the ratio of dexmedetomidine to levomedetomidine is 97/3-94/6.
Conventional operations in the operation steps of the present invention are well known to those skilled in the art and will not be described herein.
The embodiments described above are intended to illustrate the technical solutions of the present invention in detail, and it should be understood that the above-mentioned embodiments are only specific embodiments of the present invention, and are not intended to limit the present invention, and any modification, supplement or similar substitution made within the scope of the principles of the present invention should be included in the protection scope of the present invention.
Claims (3)
1. An oxytocin embedding method based on cuttlebone is characterized in that: the method comprises the steps of anaesthetizing cuttlefish, drilling holes on cuttlebone, and putting a hormone slow-release agent into the holes;
in the anesthesia process, the cuttlefish is placed in the anesthetic for 8-10 min.
The preparation method of the anesthetic comprises the following steps: taking 2-4 parts of medetomidine and 1-2 parts of oxycodone, adding 9-11 parts of deionized water, stirring for 10-14min, adding 60-65 parts of seawater, and stirring for 15-20min to obtain an anesthetic;
the mass ratio of dexmedetomidine to levomedetomidine in the anesthetic ingredient medetomidine is 97/3-94/6.
2. The method for embedding oxytocin based on cuttlebone as claimed in claim 1, wherein: the preparation method of the hormone sustained release agent comprises the following steps: adding 10-15 parts of chitosan quaternary ammonium salt into 900-1000 parts of distilled water, uniformly stirring, adding 0.1-0.2 part of GnRH powder, stirring for 10-15min, adding 2-3 parts of sodium tripolyphosphate, stirring for 30-35min, ultracentrifuging the liquid, and freeze-drying the obtained solid mixture to obtain the hormone slow-release agent.
3. The method for embedding oxytocin based on cuttlebone as claimed in claim 2, characterized in that: the preparation method of the hormone sustained release agent comprises the following steps: adding 10-15 parts of chitosan quaternary ammonium salt into 900-1000 parts of distilled water, uniformly stirring, adding 0.1-0.2 part of GnRH powder, stirring for 10-15min, then adding 5-7 parts of azodicarbonamide, stirring for 4-6min, then adding 2-3 parts of sodium tripolyphosphate, stirring for 30-35min, ultracentrifuging the liquid, and freeze-drying the obtained solid mixture to obtain the hormone slow-release agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710597599.5A CN107466912B (en) | 2017-07-21 | 2017-07-21 | Cuttlefish and cuttlebone-based oxytocin embedding method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710597599.5A CN107466912B (en) | 2017-07-21 | 2017-07-21 | Cuttlefish and cuttlebone-based oxytocin embedding method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107466912A CN107466912A (en) | 2017-12-15 |
| CN107466912B true CN107466912B (en) | 2020-01-07 |
Family
ID=60596314
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710597599.5A Active CN107466912B (en) | 2017-07-21 | 2017-07-21 | Cuttlefish and cuttlebone-based oxytocin embedding method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107466912B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0368000B1 (en) * | 1988-10-10 | 1998-01-07 | ISRAEL OCEANOGRAPHIC & LIMNOLOGICAL RESEARCH LTD. | Use of sustained-release compositions containing GnRH, LHRH or derivatives thereof |
| CN1416895A (en) * | 2002-12-02 | 2003-05-14 | 南京师范大学 | Slow releasing microballoon injection of fish mature accelerating hormone and its prepn |
| CN1686560A (en) * | 2005-04-08 | 2005-10-26 | 武汉大学 | Chitin tetra ammonium salt nano-particle, its preparation method and use |
| CN203194298U (en) * | 2012-11-15 | 2013-09-18 | 浙江海洋学院 | Squid intensification label |
| CN105836870A (en) * | 2016-05-25 | 2016-08-10 | 厦门建霖工业有限公司 | Household water treatment filter material capable of slowly releasing vitamin C and preparation method thereof |
| CN106719178A (en) * | 2016-12-05 | 2017-05-31 | 浙江海洋大学 | A kind of cephalopodous artificial ripening method |
-
2017
- 2017-07-21 CN CN201710597599.5A patent/CN107466912B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0368000B1 (en) * | 1988-10-10 | 1998-01-07 | ISRAEL OCEANOGRAPHIC & LIMNOLOGICAL RESEARCH LTD. | Use of sustained-release compositions containing GnRH, LHRH or derivatives thereof |
| CN1416895A (en) * | 2002-12-02 | 2003-05-14 | 南京师范大学 | Slow releasing microballoon injection of fish mature accelerating hormone and its prepn |
| CN1686560A (en) * | 2005-04-08 | 2005-10-26 | 武汉大学 | Chitin tetra ammonium salt nano-particle, its preparation method and use |
| CN203194298U (en) * | 2012-11-15 | 2013-09-18 | 浙江海洋学院 | Squid intensification label |
| CN105836870A (en) * | 2016-05-25 | 2016-08-10 | 厦门建霖工业有限公司 | Household water treatment filter material capable of slowly releasing vitamin C and preparation method thereof |
| CN106719178A (en) * | 2016-12-05 | 2017-05-31 | 浙江海洋大学 | A kind of cephalopodous artificial ripening method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107466912A (en) | 2017-12-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104585547B (en) | A kind of special tame and docile food feed of mandarin fish and supporting tame and docile food method | |
| CN103843701B (en) | A kind of cultural method of Bostrichthys sinensis | |
| CN102511329A (en) | Artificial induction agarwood production method from tree of Aquilaria sinensis (Lour.) Gilg by high-pressure injection | |
| CN105594635A (en) | Culture method for good-quality and high-yield catfish | |
| CN105165688A (en) | Domesticating method of wild ancherythroculter nigrocauda Yih et Wu | |
| CN102524128B (en) | Artificial reproduction method for Pseudobagras ussuriensis | |
| CN101297817A (en) | Combination for promoting carapace growth and recovery of shrimp and crab of crustacean | |
| CN102177863B (en) | Method for inducing hormone soaked loaches | |
| CN107466912B (en) | Cuttlefish and cuttlebone-based oxytocin embedding method | |
| CN104430085A (en) | Artificial reproduction method for Triplophysa yarkandensis | |
| CN103782939A (en) | Manual induced spawning method for platichthys stellatus parental fishes | |
| CN104145869B (en) | Artificial breeding method for acipenser dabryanus | |
| CN102232363B (en) | Method for artificially inducing sexual glands of Anguilla marmorata to develop maturely | |
| CN106857394A (en) | A kind of soft-shelled turtle growth promotion cultural method | |
| CN105557581A (en) | Green aquaculture method | |
| CN109618989A (en) | A kind of Micropterus salmonoides pond water quality adjusting method | |
| CN107439451B (en) | Endoconcha Sepiae long-acting physical marking method based on endoskeleton | |
| Sorach et al. | Effects of phytoecdysone on the molting period and survival rate of the blue swimming crab, Portunus pelagicus. | |
| CN105432522A (en) | Culture method of Channel catsh Lctalurus punctatus | |
| CN101559060A (en) | Medicine for preventing fish parasitic crustacean diseases, preparation method and application thereof | |
| Olufeagba et al. | Growth performance and nutrient utilization of hormonal sex-reversed male and mixed sex Oreochromis niloticus under outdoor rearing condition | |
| CN103461252A (en) | Method for rearing and maturing myxocyprinus asiaticus parent fish | |
| CN106879510B (en) | The naked induced spawning method for splitting buttocks fish in Lhasa | |
| CN110432189A (en) | A kind of wild tuna is ingested the acclimation method and artifical compound feed of artifical compound feed | |
| US20240224953A1 (en) | Silicic acid in aquaculture |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |