CN107446806A - A kind of plant leaf blade instantaneous conversion device - Google Patents
A kind of plant leaf blade instantaneous conversion device Download PDFInfo
- Publication number
- CN107446806A CN107446806A CN201710799723.6A CN201710799723A CN107446806A CN 107446806 A CN107446806 A CN 107446806A CN 201710799723 A CN201710799723 A CN 201710799723A CN 107446806 A CN107446806 A CN 107446806A
- Authority
- CN
- China
- Prior art keywords
- lower cover
- upper lid
- lid
- conversion device
- plant leaf
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000006243 chemical reaction Methods 0.000 title claims abstract description 28
- 239000012530 fluid Substances 0.000 claims abstract description 9
- 239000010985 leather Substances 0.000 claims abstract description 7
- 238000007789 sealing Methods 0.000 claims description 5
- 230000000903 blocking effect Effects 0.000 claims description 3
- 210000004027 cell Anatomy 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 230000014509 gene expression Effects 0.000 description 4
- 239000007924 injection Substances 0.000 description 4
- 238000002347 injection Methods 0.000 description 4
- 238000005086 pumping Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 230000010474 transient expression Effects 0.000 description 3
- 241000589158 Agrobacterium Species 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 238000010413 gardening Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007643 physiology damage Effects 0.000 description 1
- 210000000745 plant chromosome Anatomy 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention relates to a kind of plant leaf blade instantaneous conversion device,It includes lower cover and is rotatably fastened on the upper lid of the lower cover,The inward flange of lid and the lower cover is provided with rubber seal on described,The lower cover and the upper lid are disc,The medial surface of the upper lid and the lower cover is recessed lid inner plane and lower cover inner plane respectively,The center of the upper lid has been connected through a screw thread regulation stud,The top surface of the upper lid is stretched out in one end of the regulation stud,The other end is fixedly connected with the rounded tabletting being arranged in the upper lid inner plane,The center of the regulation stud and the center of the tabletting are separately provided for injecting the through hole of conversion fluid into lid,The one end covered is stretched out on described removably equipped with the leather sheath for being used to block the through hole in the regulation stud,The vacuum sucking holes with suck-back device are provided with and communicated with the side wall covered on described.
Description
Technical field
The present invention relates to a kind of plant leaf blade instantaneous conversion device, belong to plant genetic engineering field.
Background technology
Gene transient expression technology is that target gene is transferred into target cell within the relatively short time, is established in the cell temporary
When efficient expression system, obtain the technology of the of short duration high level expression of the target gene.Compared with stablizing expression, transient expression
Required time is short, it is not necessary to which by exogenous origin gene integrator into host plant chromosome, some for being relatively specific for gene-protein are thin
Born of the same parents' biological study.Transient expression technology has been applied in multiple kinds of crops and gardening plant in recent years, and conventional plant is instantaneous
The position of conversion is cotyledon or blade, and mode is that the conversion fluid containing Agrobacterium that will have been configured is expelled in cotyledon or blade,
If with reference to application of vacuum Agrobacterium can be made to be in close contact with plant cell before needle tubing is injected, the expression cell of acquisition not only limits
In tissue surface, transformation efficiency will be improved.
But the tiny devices not vacuumized in the market to live plant blade, experimenter are typically straight
Catcher is employed needle tubing and injected, because plant instantaneous conversion is less efficient, so needing the plant converted in experimentation
100 at least, at most thousands of, whole operation process is not only time-consuming, laborious, and have impact on the success rate of conversion.
The content of the invention
For problem present in background technology, it is an object of the invention to provide a kind of easy to operate, high conversion rate
Plant leaf blade instantaneous conversion device.
To achieve the above object, the present invention uses following technical scheme：A kind of plant leaf blade instantaneous conversion device, its feature
It is：The upper lid including lower cover and being rotatably fastened on the lower lid, the lower cover and the upper lid are disc, described
The medial surface of upper lid and the lower cover is recessed lid inner plane and lower cover inner plane respectively, lid and the lower cover on described
Inward flange be provided with rubber seal, the center of the upper lid has been connected through a screw thread regulation stud, the regulation
The top surface of the upper lid is stretched out in one end of stud, and the other end is fixed with the rounded tabletting being arranged in the upper lid inner plane
The center of connection, the center of the regulation stud and the tabletting is separately provided for injecting the through hole of conversion fluid into lid,
The vacuum sucking holes with suck-back device are provided with and communicated with the side wall covered on described.
The madial wall at the edge of the tabletting and the upper lid coordinates for gap.
A groove for being used to lay petiole is provided with the lower cover side wall, is provided with the groove for close
The strip of paper used for sealing of envelope.
The suck-back device includes shell, rotatable baffle plate is provided with the madial wall of the shell and for limiting
The location-plate of the fixed flapper extreme position, the baffle plate and the location-plate have magnetic and attracted each other.
The one end covered is stretched out on described removably equipped with the leather sheath for being used to block the through hole in the regulation stud.
For the present invention due to taking above technical scheme, it has advantages below：1st, the present invention includes lower cover and rotation connection
Be used to fasten on lower lid to cover upper lid in lower cover, inner side indent has a upper lid inner plane on upper lid, on the inside of upper lid with upper lid
Plane is arranged with adjustable tabletting in parallel, and lower cover inner plane is used to place blade, be provided with the center of upper lid with it is upper
The adjusting means of threaded connection is covered, one end of adjusting means is connected with tabletting, and the other end is located at the outside of upper lid, in the outer of upper lid
The injection orifice for injecting conversion fluid into lid is provided with and communicated with side and is removably used for the leather sheath for blocking injection orifice,
Connection is provided with the vacuum sucking holes of suck-back device in the side wall of upper lid, and reagent or conversion are injected into lid by injection orifice
Liquid, injection orifice then is stoppered with leather sheath, will be vacuumized by vacuum sucking holes in lid, therefore the present invention may be such that conversion fluid and blade
Cell fully contacts, and improves the success rate of conversion.2nd, the present invention is provided with one in lower cover side wall and is used to lay the recessed of petiole
Groove, thus petiole in the extrusion process of tabletting will not physiology damage.
Brief description of the drawings
Fig. 1 is the overall structure diagram of the present invention；
Fig. 2 is the overall half section structure diagram of the present invention；
Fig. 3 is the half section structure diagram covered in the present invention；
Fig. 4 is working position structural representation when suck-back device of the present invention vacuumizes；
Fig. 5 is the working position structural representation after suck-back device of the present invention vacuumizes.
Embodiment
The present invention is described in detail with reference to the accompanying drawings and examples.
As shown in Figures 1 to 3, the present invention includes lower cover 1 and the upper lid 2 being rotatably fastened on lower cover 1, lower cover 1 and upper lid 2
It is disc, the medial surface of upper lid 2 and lower cover 1 is recessed lid inner plane 3 and lower cover inner plane 4 respectively, in the upper He of lid 2
The inward flange of lower cover 1 is provided with rubber seal (not shown), and lower cover inner plane 4 is used to placing blade, in upper lid 2
Heart position has been connected through a screw thread regulation stud 5, and the top surface of upper lid 2 is stretched out in one end of regulation stud 5, and the other end is with being arranged in
Rounded tabletting 9 in lid inner plane 3 is fixedly connected, and the center of regulation stud 5 and the center of tabletting 9 are separately provided for
The through hole of conversion fluid is injected into lid, when regulation stud 5 rotates, can adjust distance of the tabletting 9 apart from upper lid inner plane 3.
One end that regulation stud 5 stretches out upper lid 2 is removably equipped with the leather sheath (not shown) for being used for blocking through hole.In upper lid 2
The vacuum sucking holes 7 with suck-back device 6 are provided with and communicated with side wall.
In above-described embodiment, the madial wall at the edge of tabletting 9 and upper lid 2 coordinates for gap.
In above-described embodiment, a groove 8 for being used to lay petiole is provided with the side wall of lower cover 1, is set at groove 8
There is the strip of paper used for sealing (not shown) for sealing.
In above-described embodiment, as shown in Figure 4,5, suck-back device 6 includes shell 10, is set on the madial wall of shell 10
There are rotatable baffle plate 11 and the location-plate 12 for limiting the rotation limit position of baffle plate 11, baffle plate 11 and location-plate 12 have magnetic
Property and attract each other.In pumping, suction caused by pumping is more than attraction between baffle plate 11 and location-plate 12, baffle plate 11 from
Location-plate 12 is driven, suck-back device 6 is in open mode, and after pumping terminates, suction disappears, and baffle plate 11 connects with location-plate 12
Touch, suck-back device 6 closes, during extraneous air entrance can not cover.
The course of work of the present invention is as follows：
Blade being placed on the inner plane of lower cover 1, petiole is placed in groove 8, and upper lid 2 is covered on lower cover 1, this
When on lid 2 and lower cover 1 cause one closed space of lid Inner Constitution, device for regulating rotary 13 in the presence of rubber seal
Tabletting 9 is pressed down against blade, conversion fluid is then injected into lid by through hole, through hole is stoppered with leather sheath.Pass through vacuum sucking holes 7
To be vacuumized in lid, baffle plate 11 departs from location-plate 12 during pumping, the gas discharge in lid, when stopping being evacuated, baffle plate 11 with it is fixed
Position plate 12 contacts, and gas cannot be introduced into lid.When being in a vacuum state in lid so that conversion fluid and blade cell are abundant
Contact, improve the success rate of conversion.
The present invention is only illustrated with above-described embodiment, and structure, set location and its connection of each part are all to have
Changed, on the basis of technical solution of the present invention, all improvement carried out according to the principle of the invention to individual part and equivalent
Conversion, should not be excluded outside protection scope of the present invention.
Claims (5)
- A kind of 1. plant leaf blade instantaneous conversion device, it is characterised in that：Including lower cover and rotatably it is fastened on the lower lid Upper lid, the lower cover and the upper lid are disc, and the medial surface of the upper lid and the lower cover is recessed lid respectively Inner plane and lower cover inner plane, the inward flange of lid and the lower cover is provided with rubber seal on described, the upper lid Center has been connected through a screw thread regulation stud, and the top surface of the upper lid is stretched out in one end of the regulation stud, the other end with The rounded tabletting being arranged in the upper lid inner plane is fixedly connected, in the center of the regulation stud and the tabletting The heart is separately provided for injecting the through hole of conversion fluid into lid, and being provided with and communicated with band anti-suck in the side wall covered on described fills The vacuum sucking holes put.
- A kind of 2. plant leaf blade instantaneous conversion device as claimed in claim 1, it is characterised in that：The edge of the tabletting and institute The madial wall for stating lid coordinates for gap.
- A kind of 3. plant leaf blade instantaneous conversion device as claimed in claim 1, it is characterised in that：Set in the lower cover side wall A groove for being used to lay petiole is equipped with, the groove is provided with the strip of paper used for sealing for sealing.
- A kind of 4. plant leaf blade instantaneous conversion device as claimed in claim 1, it is characterised in that：The suck-back device includes Shell, rotatable baffle plate is provided with the madial wall of the shell and for limiting determining for the flapper extreme position Position plate, the baffle plate and the location-plate have magnetic and attracted each other.
- A kind of 5. plant leaf blade instantaneous conversion device as claimed in claim 1, it is characterised in that：Stretched out in the regulation stud One end of the upper lid is removably equipped with the leather sheath for being used for blocking the through hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710799723.6A CN107446806A (en) | 2017-09-07 | 2017-09-07 | A kind of plant leaf blade instantaneous conversion device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710799723.6A CN107446806A (en) | 2017-09-07 | 2017-09-07 | A kind of plant leaf blade instantaneous conversion device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107446806A true CN107446806A (en) | 2017-12-08 |
Family
ID=60495149
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710799723.6A Pending CN107446806A (en) | 2017-09-07 | 2017-09-07 | A kind of plant leaf blade instantaneous conversion device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107446806A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101932713A (en) * | 2008-02-01 | 2010-12-29 | 拜尔创新有限责任公司 | System and method for large-scale infiltration of plants |
| CN204458431U (en) * | 2015-01-30 | 2015-07-08 | 浙江圣熠机械有限公司 | A kind of windy blower fan to anti-backflow |
| CN205317505U (en) * | 2016-01-21 | 2016-06-15 | 六盘水师范学院 | A sampling device for plant leaf sample of quarantining |
| CN205484940U (en) * | 2016-01-14 | 2016-08-17 | 南京华脉科技股份有限公司 | Mixed type optical divider case |
| CN206368812U (en) * | 2016-12-27 | 2017-08-01 | 成都摩尔生物医药有限公司 | A kind of suck-back-prevention type recirculated water multiplex vavuum pump |
| CN207193276U (en) * | 2017-09-07 | 2018-04-06 | 安阳工学院 | Plant leaf blade instantaneous conversion device |
-
2017
- 2017-09-07 CN CN201710799723.6A patent/CN107446806A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101932713A (en) * | 2008-02-01 | 2010-12-29 | 拜尔创新有限责任公司 | System and method for large-scale infiltration of plants |
| CN204458431U (en) * | 2015-01-30 | 2015-07-08 | 浙江圣熠机械有限公司 | A kind of windy blower fan to anti-backflow |
| CN205484940U (en) * | 2016-01-14 | 2016-08-17 | 南京华脉科技股份有限公司 | Mixed type optical divider case |
| CN205317505U (en) * | 2016-01-21 | 2016-06-15 | 六盘水师范学院 | A sampling device for plant leaf sample of quarantining |
| CN206368812U (en) * | 2016-12-27 | 2017-08-01 | 成都摩尔生物医药有限公司 | A kind of suck-back-prevention type recirculated water multiplex vavuum pump |
| CN207193276U (en) * | 2017-09-07 | 2018-04-06 | 安阳工学院 | Plant leaf blade instantaneous conversion device |
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| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171208 |
|
| RJ01 | Rejection of invention patent application after publication |