CN107402213B - Probe, probe device and microscope system - Google Patents
Probe, probe device and microscope system Download PDFInfo
- Publication number
- CN107402213B CN107402213B CN201710760521.0A CN201710760521A CN107402213B CN 107402213 B CN107402213 B CN 107402213B CN 201710760521 A CN201710760521 A CN 201710760521A CN 107402213 B CN107402213 B CN 107402213B
- Authority
- CN
- China
- Prior art keywords
- probe
- image
- lens
- heat absorption
- acquisition unit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
Landscapes
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Microscoopes, Condenser (AREA)
Abstract
The invention provides a probe, a probe device and a microscopic system, wherein the probe comprises a heat absorption block and an image transmission rod; the heat absorption block cools down the examined tissue, the illumination channel that link up the setting has been seted up on the heat absorption block, the one end of biography image stick stretches into in the illumination channel, and from the surface that the heat absorption block contacted with examined tissue exposes, the biography image stick includes a plurality of optical elements that arrange in proper order enlarge the formation of image. The invention can facilitate pathologists to more directly select the position of the observed slice, ensure that the final slice is the focus position, and ensure the integrity of the slice structure. In addition, the invention can reduce the number of finally dyed sections and the workload of pathologists, improve the speed of reading and reporting, and simultaneously ensure the accuracy of pathological results.
Description
Technical Field
The invention relates to the technical field of medical equipment, in particular to a probe, a probe device and a microscopic system for microscopic imaging of a slice.
Background
Along with the continuous development of clinical medicine, pathological technology has become an important means for clinical identification and diagnosis at present, wherein the rapid frozen section technology has wider clinical application range and higher application value. The principle of the rapid freeze section technique is to place living tissue under a low temperature condition and freeze it. And (5) slicing after the freezing reaches the relevant standard, and finally obtaining a pathological diagnosis result.
Compared with the conventional paraffin section technology, the quick freezing section technology is more convenient and simple to operate, and is valued in the clinical field. By using the rapid frozen section technique, the pathology department can make clear judgment on the disease condition of the patient within 15 minutes of receiving the operation specimen, and timely inform the operation doctor to make clear treatment scheme.
The frozen section is required to accurately judge and distinguish the living tissue in a short time, so that the rapid manufacture of the frozen section is a key for improving the working efficiency and guaranteeing the accuracy of the diagnosis result. In practical clinical applications, for a professional well-trained frozen section expert, only a few minutes are required from slicing to staining out a piece. However, if the treatment of the tissue is rough, the process may take several minutes to ten minutes. If multiple staining is required for a relatively large complex tissue, more time is required and this results in increased subsequent reading time.
Therefore, in view of the above problems, it is necessary to propose a further solution.
Disclosure of Invention
The invention aims to provide a probe, a probe device and a microscopic system for microscopic imaging of a slice, which are used for overcoming the defects in the prior art.
To achieve the above object, the present invention provides a probe that is applied to imaging of examined tissue, the probe including a heat absorbing block and an image transmitting rod;
the heat absorption block cools down the examined tissue, the illumination channel that link up the setting has been seted up on the heat absorption block, the one end of biography image stick stretches into in the illumination channel, and from the surface that the heat absorption block contacted with examined tissue exposes, the biography image stick includes a plurality of optical elements that arrange in proper order enlarge the formation of image.
Wherein the optical element is a lens or a light cone.
When the optical element is a lens, the image sensing rod comprises the following components which are sequentially arranged: a first lens, a second lens, a third lens, a second lens and a first lens.
And in the adjacent second lens and third lens, the second lens is arranged on the end face of the third lens.
Wherein, the surface of passing like excellent is provided with shading coating film layer.
Wherein, the illumination passageway is opened in the central point department of heat absorption piece.
To achieve the above object, the present invention provides a probe apparatus comprising: the device comprises a probe, an image transmission lighting tube and an acquisition unit;
the probe is the probe; the heat absorption block is positioned at one end of the image transmission illumination tube, and the illumination channel is communicated with the image transmission illumination tube; the image transmission rod is accommodated in the image transmission illuminating tube, and the other end of the image transmission rod is propped against the acquisition unit; the acquisition unit comprises an image sensor, and a target surface of the image sensor forms an abutting surface of the other end of the image sensing rod.
Wherein the image sensor is a camera.
The probe device further comprises a circuit board, and the circuit board is electrically connected with the camera.
The probe device further comprises two symmetrically arranged light guide rods, wherein any light guide rod extends into the illumination channel from the acquisition unit through the image transmission illumination tube and is exposed from the surface of the heat absorption block, which is in contact with the tissue to be detected.
The light guide rod in the acquisition unit bypasses the image sensor and keeps non-interference with the image sensor.
The acquisition unit further comprises a shell for protecting the image sensor, and the shell is an antifreezing shell with low thermal conductivity.
To achieve the above object, the present invention provides a microscope system comprising: a probe device, a light source device, a display device and a control device;
the probe device is the probe device, and the acquisition unit and the control device are in data transmission; the light source device provides illumination required by imaging for the probe device, and the control system controls an illumination mode output by the light source device; the display device is in data transmission with the control system and displays the image data transmitted by the control device.
The light source device comprises a plurality of LED chips, and the light source output ends of the LED chips are array output ends controlled by the control system.
The probe device further comprises a connector, and a light source input line of the light source device and a data line of the control device are connected with the connector.
The control device comprises a host and a control circuit, wherein the host is a PC or an FPGA or an ASIC.
The host computer further comprises a phase contrast processing module, and the phase contrast processing module generates a phase contrast image according to the following formula:
wherein I is the final image; i 1 A photographed image after the light guide bar at one side of the probe device is lightened, I 2 After the light guide rod at the other side of the probe device is lightened, another shot image is taken
Compared with the prior art, the invention has the beneficial effects that: the invention can facilitate pathologists to more directly select the position of the observed slice, ensure that the final slice is the focus position, and ensure the integrity of the slice structure. In addition, the invention can reduce the number of finally dyed sections and the workload of pathologists, improve the speed of reading and reporting, and simultaneously ensure the accuracy of pathological results.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments described in the present invention, and other drawings may be obtained according to the drawings without inventive effort to those skilled in the art.
FIG. 1 is a schematic diagram of a microscope system according to an embodiment of the present invention;
FIG. 2 is an enlarged schematic view of the probe apparatus of FIG. 1;
fig. 3 is a schematic internal structure of an embodiment of an image sensor according to the present invention.
Detailed Description
The present invention will be described in detail below with reference to the embodiments shown in the drawings, but it should be understood that the embodiments are not limited to the present invention, and functional, method, or structural equivalents and alternatives according to the embodiments are within the scope of protection of the present invention by those skilled in the art.
As shown in fig. 1, the present invention provides a microscope system for microscopic imaging of examined tissue extracted from a patient, and in particular, the microscope system of the present invention includes: a probe device 1, a light source device 2, a display device 3, a control device 4, and a sample stage 5.
The probe device 1 is used for imaging a tissue to be examined placed on a sample stage 5, and the probe device 1 is a contact probe device 1. Wherein the probe apparatus 1 comprises: a probe 11, a transmission illumination tube 12 and an acquisition unit 13.
As shown in fig. 2, the probe 11 is used for freezing the tissue to be examined and transmitting an image of the tissue to be examined under the condition of illumination. Wherein the probe 11 comprises a heat absorption block 111 and an image transmission rod 112.
When observing the tissue, the heat absorbing block 111 has a surface contacting the tissue, and cools the tissue under the heat conduction. In one embodiment, the heat absorbing block 111 is flat cylindrical in shape. The heat absorbing block 111 is further provided with an illumination channel penetrating in the axial direction, and the illumination channel is used for irradiating light provided in the light source device 2 onto the tissue to be inspected. Preferably, the illumination channel is opened at a central position of the heat absorbing block 111.
The imaging rod 112 is used for contacting the tissue to be examined and transmitting the image information of the tissue to be examined to the control system. One end of the image sensor bar 112 extends into the illumination channel and is exposed from the surface of the heat absorbing block 111 contacting the tissue to be examined. When the heat absorption block 111 cools the tissue to be inspected, the leaked portion of the image transmission rod 112 is synchronously contacted with the tissue to be inspected, so as to realize contact type enlarged imaging.
As shown in fig. 3, in order to implement the magnified imaging of the image sensor bar 112, the image sensor bar 112 includes a plurality of optical elements for magnified imaging arranged in sequence. The image sensor bar 112 can be controlled to have a desired magnification depending on the number and arrangement of the optical elements.
Wherein the optical element may be a lens or a light cone. In an embodiment where the optical element is a lens, the lens comprises: a first lens 1121, a second lens 1122, and a third lens 1123. At this time, the lenses are arranged in order of the first lens 1121, the second lens 1122, the third lens 1123, the second lens 1122, and the first lens 1121.
Specifically, the first lens 1121 and the second lens 1122 have a light-conductive pitch therebetween, and the third lens 1123 has a light-conductive pitch therebetween. In order to realize that light is transmitted from the first lens 1121 at the incident end to the first lens 1121 at the emergent end, one side end surface of the first lens 1121 is a concavely arranged curved surface, and the other side end surface is a convexly arranged curved surface; the end face of one side of the second lens 1122 is a curved surface which is arranged in a convex manner, and the end face of the other side is a curved surface which is arranged in a concave manner; the end face of one side of the third lens 1123 is a curved surface which is arranged in a convex manner, and the end face of the other side is a plane.
In the adjacent first lens 1121 and second lens 1122, the convex end surface of the first lens 1121 is disposed opposite to the convex curved surface of the second lens 1122. In the adjacent second lens 1122 and third lens 1123, the curved surface of the concave arrangement of the second lens 1122 is mounted on the convex arrangement end surface of the third lens 1123. In the adjacent third lenses 1123, the planar end surfaces of the third lenses 1123 are disposed opposite to each other.
Thus, in the above embodiment, the image sensor bar 112 is designed according to the center wavelength of 510nm, and the image sensor bar 112 composed of 6 lenses can reach a field of view of 1mm by 1mm, and a resolution of 1.4um, and a magnification of 1.
In addition, in order to prevent stray light or excitation light from entering the microscopic system and interfering the result, a light shielding coating layer is further disposed on the outer surface of the image transmission rod 112.
The image-transmitting illumination tube 12 is located between the acquisition unit 13 and the heat absorbing block 111. Wherein, the heat absorbing block 111 is located at one end of the image transmission illumination tube 12, and the illumination channel is communicated with the image transmission illumination tube 12. Meanwhile, the image sensing rod 112 is accommodated in the image sensing illumination tube 12, and a certain gap is kept between the image sensing rod 112 and the image sensing illumination tube 12. Preferably, the image sensing rod 112 is coaxially disposed with the image sensing illumination tube 12.
The acquisition unit 13 is configured to receive the image information transmitted by the image sensor 112 and feed the image information back to the control device 4. The acquisition unit 13 comprises an image sensor 131 and a housing 130 for protecting the image sensor 131, wherein the housing 130 adopts an antifreezing housing with low thermal conductivity. The target surface of the image sensor 131 forms an abutment surface of the other end of the image sensor bar 112. Therefore, the target surface of the image sensor 131 is directly contacted with the image sensor 112, so that the acquisition unit 13 directly detects the end surface of the image sensor 112, a lens imaging step is omitted, and the final resolution is determined by the sensor pixels.
In one embodiment, the image sensor 131 is a camera having a target surface 1311 for receiving subject tissue image information conveyed by the imaging wand 112. Meanwhile, the acquisition unit 13 further includes a circuit board 132 for controlling the camera, and the circuit board 132 is electrically connected to the camera. The camera and circuit board 132 has hot-plug data and power lines, which ensures that the probe is disconnected from the wire.
In the foregoing embodiment, the camera may specifically be a lens module of a smart phone. Through the lens module of the mobile phone, the acquisition unit 13 has the advantages of high resolution (small pixels), low price, large target surface size, capability of covering the large-size image transmission rod 112, formation of a large field of view, high resolution imaging effect and the like.
Further, in order to achieve a data transmission between the acquisition unit 13 and the control device 4, the probe device 1 further comprises a connector 14, the data line 41 of the control device 4 and the data line of the camera being connected to the connector 14.
In addition, the probe device 1 further comprises two symmetrically arranged light guide rods 15, and the two light guide rods 15 are used for realizing phase contrast imaging of the invention. Specifically, any one of the light guide rods 15 extends from the collection unit 13, passes through the gap in the image transmission illumination tube 12, extends into the illumination channel, and is exposed from the surface of the heat absorption block 111 contacting the slice. In order to avoid interference of the light guide bar 15 with the image sensor 131, the light guide bar 15 located in the acquisition unit 13 bypasses the image sensor 131 and maintains non-interference with the image sensor 131.
The light source device 2 is used for providing illumination required for imaging for the probe device 1. Specifically, the light source device 2 includes a plurality of LED chips, and the light source output ends of the plurality of LED chips are array output ends controlled by the control system. Thus, different illumination modes can be selected by the control device 4, and imaging can be performed in different modes. The array output end is connected with the joint 14 of the probe device 1 through a light source input line 21, and the light guide rod 15 can be coupled with the light source input line 21 according to the requirement.
The control device 4 is configured to receive the image information acquired by the acquisition unit 13, and calculate and analyze the image information. Specifically, the control device 4 includes a host and a control circuit, where the host may be a high-performance PC, or may be a circuit board such as an FPGA or an ASIC. At the same time, the control device 4 also controls the acquisition unit 13 and the light source device 2.
In addition, to achieve phase contrast imaging, the host computer further includes a phase contrast processing module that generates a phase contrast image according to the following formula:
wherein I is the final image; i 1 A photographed image after the light guide bar at one side of the probe device is lightened, I 2 And after the light guide rod at the other side of the probe device is lightened, shooting another image.
And I in formula (A) 1 -I 2 And I 1 +I 2 Representing the addition or subtraction of the intensity of each pixel point in two images, e.g. image I 1 A pixel matrix of 256 x 256, I 2 Also 256 x 256 pixels matrix, then I 1 -I 2 Light intensity of pixel points with identical coordinates in two images is subtracted, I 1 +I 2 The light intensities representing the same coordinate pixel points in the two images are added, and I in the invention 1 And I 2 The pixels of the two images are exactly the same, and thus the proportional image I is calculated by the formula (a).
The display device 3 is used for high-resolution display of the host output image. Specifically, the display device 3 includes a display that is in data transmission with the control system and displays the image data transmitted by the control device 4.
Based on the microscopic system with the structure, the invention has an autofluorescence microscopic mode and a dyeing-free differential phase contrast microscopic mode.
When the self-fluorescence microscopy mode is required to be selected, the sample is sliced on a slicing machine for several times, and then the examined tissue with smooth and even surface is obtained. The probe is attached to the surface of the detected tissue, the heat absorption block cools the detected tissue, and the probe observes the central position. At this time, when the control device controls the light source device to emit excitation light necessary for autofluorescence, for example, when it is necessary to observe autofluorescence of FAD, blue light at 445nm is used as the excitation light. Thus, autofluorescence of FAD in the sample can be observed using an image sensor bar that filters 445 nm.
The autofluorescence microscopic mode has the advantages that the autofluorescence provides a distribution image of specific molecules in the tissue, compared with the autofluorescence in the normal temperature condition, the autofluorescence of the frozen section has the characteristic of high quantum yield, which is about 10 times of the normal temperature, so that the requirement on a camera is greatly reduced, and the acquisition speed is accelerated. And autofluorescence is an endogenous fluorescent property that can be used to assess the energy metabolism state of a cell. For example, NADH and FAD indicate the oxidative and reductive states, respectively, of a cell, wherein the intensity ratio is defined as the redox ratio, indicating the energy metabolic state of the cell.
When it is desired to select a dye-free differential phase contrast microscopy mode, the phase contrast imaging mode does not require observation of the excited fluorescence in the examined tissue. When the phase contrast imaging is carried out, two light guide rods need to be used for sequentially lighting, namely, after one side light guide rod is lighted, an image I is shot 1 Then the other side light guide rod is lightened, and another image I is shot 2 . The two images are subjected to phase contrast processing according to the following formula:the generation of the phase contrast image can be completed.
The dye-free color difference phase-contrast microscopic mode has the advantages that the structural distribution information of the sample can be provided, and the differential phase-contrast mode can provide a relief effect, so that tiny deformation can be displayed. The phase contrast is sensitive to the refractive index of the examined tissue and can be effectively highlighted to the tissue or cell boundary.
Furthermore, more importantly, the two imaging modes have very low time requirements, can image in real time, finish detection while cooling the examined tissue, and assist doctors in judging whether the repositioning is worth dyeing. Therefore, once the doctor is assisted to find the pathological tissue, the frozen section work is completed, the work efficiency is improved, the waiting time of the clinician is shortened, and the pain of the patient is relieved.
In summary, the invention can facilitate pathologists to more directly select the position of the observed slice, ensure that the final slice is the focal position, and ensure the structural integrity of the slice. In addition, the invention can reduce the number of finally dyed sections and the workload of pathologists, improve the speed of reading and reporting, and simultaneously ensure the accuracy of pathological results.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.
Claims (15)
1. A probe for microscopic imaging of a slice, the probe comprising a heat absorbing block and an image transmitting rod;
the heat absorption block is used for cooling detected tissues of the slice, an illumination channel which is communicated is formed in the heat absorption block, one end of the image transmission rod extends into the illumination channel and is exposed from the surface of the heat absorption block, which is contacted with the detected tissues, the image transmission rod comprises a plurality of optical elements which are sequentially arranged and used for amplifying and imaging, the optical elements are lenses or light cones, when the heat absorption block is used for cooling the detected tissues, the exposed part of the image transmission rod is synchronously contacted with the detected tissues to realize contact type amplified imaging, and the outer surface of the image transmission rod is provided with a shading coating layer.
2. The probe of claim 1, wherein when the optical element is a lens, the image sensor bar comprises, in order: a first lens, a second lens, a third lens, a second lens and a first lens.
3. The probe of claim 2, wherein, of the second and third lenses that are adjacent, the second lens is mounted on an end face of the third lens.
4. The probe of claim 1, wherein the illumination channel is open at a central location of the heat sink block.
5. A probe apparatus, the probe apparatus comprising: the device comprises a probe, an image transmission lighting tube and an acquisition unit;
the probe is the probe according to any one of claims 1 to 4; the heat absorption block is positioned at one end of the image transmission illumination tube, and the illumination channel is communicated with the image transmission illumination tube; the image transmission rod is accommodated in the image transmission illuminating tube, and the other end of the image transmission rod is propped against the acquisition unit; the acquisition unit comprises an image sensor, and a target surface of the image sensor forms an abutting surface of the other end of the image sensing rod.
6. The probe apparatus of claim 5 wherein the image sensor is a camera.
7. The probe device of claim 6, further comprising a circuit board electrically connected to the camera.
8. The probe device according to claim 5, further comprising two symmetrically arranged light guide rods, wherein any one of the light guide rods extends from the acquisition unit, passes through the image transmission illumination tube, extends into the illumination channel, and is exposed from a surface of the heat absorption block, which is in contact with the tissue to be examined.
9. The probe apparatus of claim 5, wherein a light guide bar in the acquisition unit bypasses the image sensor and remains non-interfering with the image sensor.
10. The probe apparatus of claim 5, wherein the acquisition unit further comprises a housing protecting the image sensor, the housing being a freeze-protected and low thermal conductivity housing.
11. A microscopy system, the microscopy system comprising: a probe device, a light source device, a display device and a control device;
the probe device is a probe device according to any one of claims 5 to 10, and the acquisition unit is in data transmission with the control device; the light source device provides illumination required by imaging for the probe device, and the control device controls an illumination mode output by the light source device; the display device is in data transmission with the control device and displays the image data transmitted by the control device.
12. The microscopy system of claim 11, wherein the light source means comprises a plurality of LED chips, the light source outputs of the plurality of LED chips being array outputs controlled by the control means.
13. The microscopy system of claim 11, wherein the probe means further comprises a connector, the light source input line of the light source means and the data line of the control means being connected to the connector.
14. The microscopy system of claim 11, wherein the control means comprises a host and a control circuit, the host being a PC or FPGA or ASIC.
15. The microscopy system of claim 14, wherein the host further comprises a phase contrast processing module that generates a phase contrast image according to the following formula:
；
wherein I is the final image; i 1 A photographed image after the light guide bar at one side of the probe device is lightened, I 2 And after the light guide rod at the other side of the probe device is lightened, shooting another image.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710760521.0A CN107402213B (en) | 2017-08-29 | 2017-08-29 | Probe, probe device and microscope system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710760521.0A CN107402213B (en) | 2017-08-29 | 2017-08-29 | Probe, probe device and microscope system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107402213A CN107402213A (en) | 2017-11-28 |
| CN107402213B true CN107402213B (en) | 2023-09-22 |
Family
ID=60396799
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710760521.0A Active CN107402213B (en) | 2017-08-29 | 2017-08-29 | Probe, probe device and microscope system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107402213B (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999004694A1 (en) * | 1997-07-21 | 1999-02-04 | Laser Industries Ltd. | A system for laparoscopic ultrasound guidance of a surgical cryo-probe |
| US6059820A (en) * | 1998-10-16 | 2000-05-09 | Paradigm Medical Corporation | Tissue cooling rod for laser surgery |
| US6413252B1 (en) * | 1998-02-26 | 2002-07-02 | Lucid, Inc. | Confocal microscope for facilitating cryosurgery of tissue |
| WO2007123684A2 (en) * | 2006-04-04 | 2007-11-01 | Biotime, Inc. | Methods and devices for thawing and/or imaging frozen biological samples |
| CN103975229A (en) * | 2011-09-29 | 2014-08-06 | 迈阿密大学 | Ultra-rapid diagnostic tissue preparation as an alternative to frozen section |
| CN104765138A (en) * | 2015-04-17 | 2015-07-08 | 南京理工大学 | Multi-mode micro-imaging system and method based on LED array |
| WO2017012555A1 (en) * | 2015-07-20 | 2017-01-26 | 徐敏 | Photon structure and chemometrics pathologic system |
| CN207351910U (en) * | 2017-08-29 | 2018-05-11 | 苏州精观医疗科技有限公司 | Probe, probe apparatus and microscopic system |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6530882B1 (en) * | 2000-06-30 | 2003-03-11 | Inner Vision Imaging, L.L.C. | Endoscope having microscopic and macroscopic magnification |
| US20060155267A1 (en) * | 2005-01-10 | 2006-07-13 | Nir Berzak | Thermal mapping of a cryoablation volume, for image-guided cryosurgery |
| US20080033455A1 (en) * | 2006-08-03 | 2008-02-07 | Rassman William R | Hair extraction device and method for its use |
| US11154186B2 (en) * | 2015-07-31 | 2021-10-26 | University Of Utah Research Foundation | Devices, systems, and methods for imaging and treating a selected tissue |
-
2017
- 2017-08-29 CN CN201710760521.0A patent/CN107402213B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999004694A1 (en) * | 1997-07-21 | 1999-02-04 | Laser Industries Ltd. | A system for laparoscopic ultrasound guidance of a surgical cryo-probe |
| US6413252B1 (en) * | 1998-02-26 | 2002-07-02 | Lucid, Inc. | Confocal microscope for facilitating cryosurgery of tissue |
| US6059820A (en) * | 1998-10-16 | 2000-05-09 | Paradigm Medical Corporation | Tissue cooling rod for laser surgery |
| WO2007123684A2 (en) * | 2006-04-04 | 2007-11-01 | Biotime, Inc. | Methods and devices for thawing and/or imaging frozen biological samples |
| CN103975229A (en) * | 2011-09-29 | 2014-08-06 | 迈阿密大学 | Ultra-rapid diagnostic tissue preparation as an alternative to frozen section |
| CN104765138A (en) * | 2015-04-17 | 2015-07-08 | 南京理工大学 | Multi-mode micro-imaging system and method based on LED array |
| WO2017012555A1 (en) * | 2015-07-20 | 2017-01-26 | 徐敏 | Photon structure and chemometrics pathologic system |
| CN207351910U (en) * | 2017-08-29 | 2018-05-11 | 苏州精观医疗科技有限公司 | Probe, probe apparatus and microscopic system |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107402213A (en) | 2017-11-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Thekkek et al. | Optical imaging for cervical cancer detection: solutions for a continuing global problem | |
| EP2057936B1 (en) | Method and system for characterization and mapping of tissue lesions | |
| Shin et al. | A fiber-optic fluorescence microscope using a consumer-grade digital camera for in vivo cellular imaging | |
| Pierce et al. | A pilot study of low-cost, high-resolution microendoscopy as a tool for identifying women with cervical precancer | |
| Cicchi et al. | Time-and spectral-resolved two-photon imaging of healthy bladder mucosa and carcinoma in situ | |
| Gareau et al. | Sensitivity and specificity for detecting basal cell carcinomas in Mohs excisions with confocal fluorescence mosaicing microscopy | |
| CN105182514B (en) | Based on LED light source without lens microscope and its image reconstructing method | |
| Chernavskaia et al. | Beyond endoscopic assessment in inflammatory bowel disease: real-time histology of disease activity by non-linear multimodal imaging | |
| Devi et al. | Detecting cervical cancer progression through extracted intrinsic fluorescence and principal component analysis | |
| CN1870929A (en) | Automated endoscopy device, diagnostic method, and uses | |
| Gareau et al. | Tri-modal confocal mosaics detect residual invasive squamous cell carcinoma in Mohs surgical excisions | |
| Li et al. | Label-free assessment of premalignant gastric lesions using multimodal nonlinear optical microscopy | |
| Thekkek et al. | Modular video endoscopy for in vivo cross-polarized and vital-dye fluorescence imaging of Barrett’s-associated neoplasia | |
| CN107402213B (en) | Probe, probe device and microscope system | |
| Graham et al. | Advances in upper gastrointestinal endoscopy | |
| Browning et al. | Design of a modified endoscope illuminator for spectral imaging of colorectal tissues | |
| Hu et al. | Design and validation of an intraoperative autofluorescence lifetime imaging device | |
| Grant et al. | High-resolution microendoscope for the detection of cervical neoplasia | |
| CN207351910U (en) | Probe, probe apparatus and microscopic system | |
| Upile et al. | Optical diagnostic techniques in the head and neck | |
| Gawedzinski et al. | Toward development of a large field-of-view cancer screening patch (CASP) to detect cervical intraepithelial neoplasia | |
| Bae et al. | Feasibility studies of multimodal nonlinear endoscopy using multicore fiber bundles for remote scanning from tissue sections to bulk organs | |
| CN206920694U (en) | One kind is in body microscopic system | |
| Wu et al. | Preliminary study of detecting neoplastic growths in vivo with real time calibrated autofluorescence imaging | |
| Browning et al. | Illuminating the optical properties of an LED-based spectral light source for hyperspectral endoscopy |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information |
Address after: 215127 No. 104-106, Wanlong building, No. 29, Xinfa Road, Suzhou area, China (Jiangsu) pilot Free Trade Zone, Suzhou City, Jiangsu Province, 1st floor, auxiliary building (this address shall not be engaged in retail) Applicant after: Suzhou Oupu Mandi Technology Co.,Ltd. Address before: 215000 unit 302, building A6, biomedical industrial park, 218 Xinghu street, Suzhou Industrial Park, Jiangsu Province Applicant before: SUZHOU JINGGUAN MEDICAL TECHNOLOGY CO.,LTD. |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant |