CN107328935B - Abnormal prothrombin detection kit and device - Google Patents
Abnormal prothrombin detection kit and device Download PDFInfo
- Publication number
- CN107328935B CN107328935B CN201710390260.8A CN201710390260A CN107328935B CN 107328935 B CN107328935 B CN 107328935B CN 201710390260 A CN201710390260 A CN 201710390260A CN 107328935 B CN107328935 B CN 107328935B
- Authority
- CN
- China
- Prior art keywords
- pipe
- hole
- detector bar
- antibody
- preinstalled
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 238000001514 detection method Methods 0.000 title claims abstract description 24
- 230000002159 abnormal effect Effects 0.000 title claims abstract description 17
- 102100015239 F2 Human genes 0.000 title claims abstract description 16
- 229940039716 Prothrombin Drugs 0.000 title claims abstract description 16
- 108010094028 Prothrombin Proteins 0.000 title claims abstract description 16
- 239000007788 liquid Substances 0.000 claims abstract description 32
- 102000004965 antibodies Human genes 0.000 claims abstract description 31
- 108090001123 antibodies Proteins 0.000 claims abstract description 31
- 239000000758 substrate Substances 0.000 claims abstract description 14
- 238000004140 cleaning Methods 0.000 claims abstract description 12
- 239000012482 calibration solution Substances 0.000 claims description 11
- 239000007983 Tris buffer Substances 0.000 claims description 8
- 102000005614 monoclonal antibodies Human genes 0.000 claims description 8
- 108010045030 monoclonal antibodies Proteins 0.000 claims description 8
- 238000010790 dilution Methods 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 5
- 108090000790 Enzymes Proteins 0.000 claims description 5
- ORILYTVJVMAKLC-UHFFFAOYSA-N Adamantane Chemical compound C1C(C2)CC3CC1CC2C3 ORILYTVJVMAKLC-UHFFFAOYSA-N 0.000 claims description 4
- 239000006249 magnetic particle Substances 0.000 claims description 4
- 239000008363 phosphate buffer Substances 0.000 claims description 4
- 238000009938 salting Methods 0.000 claims description 4
- -1 Oxygen cyclohexane disodium Chemical compound 0.000 claims 1
- 239000006210 lotion Substances 0.000 claims 1
- 239000000243 solution Substances 0.000 description 10
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 238000009434 installation Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000006011 modification reaction Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 210000004369 Blood Anatomy 0.000 description 1
- 210000001124 Body Fluids Anatomy 0.000 description 1
- 229950003499 FIBRIN Drugs 0.000 description 1
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 1
- 102000009123 Fibrin Human genes 0.000 description 1
- 108010073385 Fibrin Proteins 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 230000000875 corresponding Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
Abstract
The present invention relates to abnormal prothrombin detection kit and devices, belong to the field of medical instrument technology comprising: detector bar is provided with the hole of multiple same sizes；The multiple pipes being separately provided, the multiple pipe include cleaning liquid pipe, coated antibody pipe, labelled antibody pipe and substrate liquid pipe；Wherein each pipe upper end having the same, each pipe is removably installed in each hole of the detector bar, the present invention can arbitrarily exchange position of the pipe on detector bar as needed, can reduce testing cost, and only can realize multiple Parallel testing with a detector bar.
Description
Technical field
The present invention relates to medical equipment technical fields, more particularly to abnormal prothrombin (DCP) detection kit.
Background technique
The apparatus for automatically measuring for automatically analyzing special component in body fluid such as blood of human body has been disclosed at present, and
And also disclose all kinds of detector bars suitable for these apparatus for automatically measuring.These detector bars are usually on a strip structure
It is fixedly disposed multiple reactive tanks and/or multiple for being preinstalled with the slot of different solutions, reagent.
Under normal conditions, apparatus for automatically measuring has fixed detection program, and measurement device can be by pre- in the detection process
Program is determined according to the position (for example, according to cis-position of the slot of one end of detector bar to the other end) of slot each in detector bar come automatically
Identify different solution or reagent.At this point, specific apparatus for automatically measuring corresponds to specific detector bar.
However, needing the position of each solution or reagent on change detection item under specific circumstances.At this time or need again
The preset program in measurement device is set, or needs to redesign detector bar.Because each slot of detector bar is fixedly
Be set to the specific position of detector bar, corresponding solution in each slot, reagent position immobilize, cannot optionally be become
It changes.In the case where each slot pre-installs different types of solution, reagent, problems are especially prominent.
Therefore, this field needs to solve new departure of such technical problem.
Summary of the invention
To solve problems of the prior art, the present invention provides abnormal prothrombin (DCP) detection kit, energy
Enough rapidly separation detection abnormal prothrombins, while the different location of detection slot can also be replaced mutually.In addition, of the invention
Detection kit can also reduce testing cost, and only can realize multiple (for example, twice) Parallel testing with a detector bar.
Specifically, the present invention includes following technical solution.
A kind of abnormal prothrombin detection kit comprising: detector bar is provided with the hole of multiple same sizes；It is single
The multiple pipes being solely arranged, the multiple pipe include cleaning liquid pipe, coated antibody pipe, labelled antibody pipe and substrate liquid pipe；It is wherein each
The pipe upper end having the same, each pipe are removably installed in each hole of the detector bar.
Preferably, one end of the detector bar is provided with the sample slot for mixing antibody with sample.
Preferably, have 6 cleaning liquid pipes, 2 coated antibody pipes, 2 labelled antibody pipes and/or
2 substrate liquid pipes.
Preferably, the cleaning liquid pipe is preinstalled with Tris buffer；The coated antibody pipe is preinstalled with magnetic particle envelope
The anti-human DCP monoclonal antibody of mouse, phosphate buffer and BSA；The labelled antibody pipe is preinstalled with the anti-human DCP of mouse of AP enzyme label
Monoclonal antibody, Tris buffer and BSA；And/or the substrate liquid pipe is preinstalled with 3- (2- spiral adamantane) -4- methoxyl group -
4- (3- phosphorus oxygen acyl)-phenyl -1,2- dioxane disodium salting liquid.
Preferably, the hole has the first hole and the second hole, and the upper end and first hole are adapted, and the pipe is also
With the outer profile being adapted with second hole.
Preferably, it is provided with wherein being located at below the upper end of the pipe on one section of the outer profile around described outer
The protrusion structure of profile surrounding, the prominent structure are used to the pipe being removably installed in the detector bar.
Preferably, the prominent structure is arc-shaped.
Preferably, further comprising: being used to accommodate the first calibration solution bottle of the first calibration solution；For accommodating the second calibration
Second calibration solution bottle of liquid；With the calibration dilution bottle for accommodating calibration dilution.
Detailed description of the invention
Fig. 1 is the perspective view of detector bar of the invention.
Fig. 2 is the top view of embodiment illustrated in fig. 1 of the invention.
Fig. 3 is the cross-sectional view of embodiment illustrated in fig. 1 of the invention.
Fig. 4 is the G partial view of Fig. 3 of the invention.
Fig. 5 is the perspective view of the detector bar of the invention without piping.
Fig. 6 is the perspective view of pipe of the invention.
Description of symbols:
10- detector bar,
The hole 11-,
The first hole 111-,
The second hole 112-,
12- sample slot,
20- pipe,
201- cleans liquid pipe,
202- coated antibody pipe,
203- labelled antibody pipe,
204- substrate liquid pipe,
The upper end 21-,
22- outer profile,
221- protrudes structure.
It should be noted that identical appended drawing reference indicates the same or similar meaning in the present invention.For example, Fig. 1 and
Appended drawing reference 2 in Fig. 2 indicates the first transposons enzyme binding sequence.
Specific embodiment
The existing various exemplary embodiment that the present invention will be described in detail, the detailed description are not considered as to limit of the invention
System, and it is understood as the more detailed description to certain aspects of the invention, characteristic and embodiment.
The present invention provides abnormal prothrombin (DCP) detection kit, being capable of rapidly separation detection exception fibrin ferment
Original, while the different location of detection slot can also be replaced mutually.Detection kit of the invention can also reduce testing cost, and
And multiple Parallel testing only can be realized with a detector bar.
The present invention provides a kind of abnormal prothrombin detection kit comprising: detector bar 10 is provided with multiple identical
The hole 11 of size；The multiple pipes 20 being separately provided, the multiple pipe 20 include cleaning liquid pipe 201, coated antibody pipe 202, label
Antibody pipe 203 and substrate liquid pipe 204；Wherein each pipe 20 upper end 21 having the same, each pipe 20 are removably pacified
In each hole 11 loaded on the detector bar 10, position of the pipe 20 on detector bar 10 can be arbitrarily exchanged as needed.
Below with reference to the embodiment that the present invention will be described in detail of the attached drawing for illustrating detector bar 10.
Fig. 1 is the perspective view of detector bar of the invention.Please refer to shown in Fig. 1, detector bar 10 of the invention be equipped with
Different solution can be injected in multiple pipes 20 as different solution conduits, example for its multiple individual pipe 20 being detachably connected
Such as, the pipe 20 in the present embodiment includes cleaning liquid pipe 201, coated antibody pipe 202, labelled antibody pipe 203 and substrate liquid pipe 204,
Wherein the quantity of each solution conduit can be for one or multiple.
Preferably, there are described 202,2 labelled antibodies of coated antibody pipe of 6 cleaning liquid pipes 201,2
Pipe 203 and/or 2 substrate liquid pipes 204.
It is further preferred that wherein, the cleaning liquid pipe 201 is preinstalled with Tris buffer；The coated antibody pipe 202 is pre-
The anti-human DCP monoclonal antibody of mouse, phosphate buffer and BSA equipped with magnetic particle envelope；The labelled antibody pipe 203 is preinstalled with
The anti-human DCP monoclonal antibody of mouse, Tris buffer and the BSA of AP enzyme label；And/or the substrate liquid pipe 204 is preinstalled with 3- (2-
Spiral adamantane) -4- methoxyl group -4- (3- phosphorus oxygen acyl)-phenyl -1,2- dioxane disodium salting liquid.
Fig. 2 is the top view of embodiment illustrated in fig. 1 of the invention.It please refers to shown in Fig. 2, the present invention has described in 3
Clean liquid pipe 201, the 1 coated antibody pipes 202, the 1 labelled antibody pipe 203 and 1 substrate liquid pipe 204.
Preferably, one end of the detector bar 10 is provided with the sample slot 12 for mixing antibody with sample, should
Sample slot 12 can be fixed on detector bar 10, can also be detachably arranged on detector bar 10.
Fig. 3 is the cross-sectional view of embodiment illustrated in fig. 1 of the invention；Fig. 4 is the G partial view of Fig. 3 of the invention.Fig. 5 is
The perspective view of detector bar without piping of the invention.
It please referring to shown in Fig. 3, Fig. 4 and Fig. 5, the hole 11 on detector bar 10 has the first hole 111 and the second hole 112,
The upper end 21 is adapted with first hole 111, and the pipe 20 also has the outer profile being adapted with second hole 112
22.The first hole 111 and the second hole 112 axis of symmetry having the same in the present embodiment, the first hole 111 are slightly larger than the second hole
112, it is needed when the pipe 20 of this structure is installed on detector bar 10 correspondingly, the upper end 21 of pipe 20 is again slightly greater than outer profile 22
It is packed into downwards from the top of the detector bar 10, after loading, the upper end 21 of pipe 20 is sunk in the first hole 111, can be made in this way
The upper surface of pipe 20 is concordant with the upper surface of detector bar 10, and joint strip is easy to use to be sealed each pipe.It should be noted that the
One hole 111 and the second hole 112 also can have the different axis of symmetry, as long as at this point, upper end 21 and the outer profile 22 of pipe 20
Also there is the different axis of symmetry, and be adapted with the first hole 111 and the second hole 112.
As preferred embodiment, as shown in Figure 4, described in 21 one section of lower section of the upper end of the pipe 20
The protrusion structure 221 around 22 surrounding of outer profile is provided on outer profile 22, the prominent structure 221 is used for the pipe 20
It is removably installed on the detector bar 10.It can make prominent structure 221 by applying certain pressure to pipe 20 when installation
Certain flexible deformation occurs for the wall or detector bar 10 of the pipe 20 at place, so that prominent structure 221 can pass through the second hole 112,
And be stuck at the lower end surface in the second hole 112, in this way, pipe 20 easily will not be detached from and cause the accident of solution from detector bar 10
Spill.
As shown in fig. 6, for the ease of pipe installation, it is further preferred that the prominent structure 221 is arc-shaped.Certainly, prominent
Structure 221 or cusped edge shape or other shapes, but in comparison, when installing or dismantling pipe, the protrusion of arc
Structure 221 is easier to enter in the second hole 112.
Preferably, abnormal prothrombin detection kit of the present invention further comprises: for accommodating the of the first calibration solution
One calibration solution bottle；For accommodating the second calibration solution bottle of the second calibration solution；With the calibration dilution for accommodating calibration dilution
Bottle.
Although illustrating the present invention referring to illustrative embodiments, but it is to be understood that it is public that the present invention is not limited to institutes
The illustrative embodiments opened.It should be readily apparent to one skilled in the art that on the basis of claims, without departing substantially from the present invention
Purport and spirit in the case where, various modifications and equivalent replacement can be carried out.Therefore, the range of claims should meet most
Wide in range explanation, to include all modifications, equivalent structure and function.
Claims (7)
1. a kind of abnormal prothrombin detection kit, characterized in that it comprises:
Detector bar (10), is provided with the hole (11) of multiple same sizes, and the hole (11) has the first hole (111) and the second hole
(112)；
The multiple pipes (20) being separately provided, the multiple pipe (20) include cleaning liquid pipe (201), coated antibody pipe (202), label
Antibody pipe (203) and substrate liquid pipe (204), and the cleaning liquid pipe (201) is preinstalled with Tris buffer, the coated antibody pipe
(202) the anti-human DCP monoclonal antibody of mouse, phosphate buffer and the BSA of magnetic particle envelope, the labelled antibody pipe are preinstalled with
(203) the anti-human DCP monoclonal antibody of mouse, Tris buffer and the BSA or the substrate liquid pipe (204) of AP enzyme label are preinstalled with
It is preinstalled with 3- (2- spiral adamantane) -4- methoxyl group -4- (3- phosphorus oxygen acyl)-phenyl -1,2- dioxane disodium salting liquid；Respectively
The multiple pipe (20) upper end having the same (21), the upper end (21) and first hole (111) are adapted, described
Multiple pipes (20) also have the outer profile (22) being adapted with second hole (112), one section of upper end (21) lower section
The protrusion structure (221) around the outer profile (22) surrounding is provided on outer profile (22), the prominent structure (221) is set as
It is stuck in after second hole (112) at the lower end surface of second hole (112), each the multiple pipe (20) is removably pacified
In each hole (11) loaded on the detector bar (10), the upper end (21) of the multiple pipe (20) is set as described when sinking to
When first hole (111) is interior, keep the upper surface of the multiple pipe (20) concordant with the upper surface of detector bar (10).
2. abnormal prothrombin detection kit according to claim 1, which is characterized in that the wherein detector bar (10)
One end be provided with the sample slot (12) for mixing antibody with sample.
3. abnormal prothrombin detection kit according to claim 1 or 2, which is characterized in that it is described clear with 6
Lotion pipeline (201), 2 coated antibody pipes (202), 2 labelled antibody pipes (203) and/or 2 substrate liquid pipes
(204)。
4. abnormal prothrombin detection kit according to claim 1 or 2, which is characterized in that wherein,
The cleaning liquid pipe (201) is preinstalled with Tris buffer；
The anti-human DCP monoclonal antibody of mouse that the coated antibody pipe (202) is preinstalled with magnetic particle envelope, phosphate buffer and
BSA；
The labelled antibody pipe (203) is preinstalled with the anti-human DCP monoclonal antibody of mouse, Tris buffer and the BSA of AP enzyme label；And
The substrate liquid pipe (204) is preinstalled with 3- (2- spiral adamantane) -4- methoxyl group -4- (3- phosphorus oxygen acyl)-phenyl -1,2- two
Oxygen cyclohexane disodium salting liquid.
5. abnormal prothrombin detection kit according to claim 1, which is characterized in that the prominent structure (221)
It is set as the pipe (20) being removably installed on the detector bar (10).
6. abnormal prothrombin detection kit according to claim 5, which is characterized in that the prominent structure (221)
It is arc-shaped.
7. abnormal prothrombin detection kit according to claim 1 or 2, which is characterized in that further comprising:
For accommodating the first calibration solution bottle of the first calibration solution；
For accommodating the second calibration solution bottle of the second calibration solution；With
For accommodating the calibration dilution bottle of calibration dilution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710390260.8A CN107328935B (en) | 2017-05-27 | 2017-05-27 | Abnormal prothrombin detection kit and device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710390260.8A CN107328935B (en) | 2017-05-27 | 2017-05-27 | Abnormal prothrombin detection kit and device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107328935A CN107328935A (en) | 2017-11-07 |
| CN107328935B true CN107328935B (en) | 2019-03-26 |
Family
ID=60193119
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710390260.8A Active CN107328935B (en) | 2017-05-27 | 2017-05-27 | Abnormal prothrombin detection kit and device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107328935B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5559002A (en) * | 1989-11-17 | 1996-09-24 | Laboratoires Biotrol | Apparatus for automatically carrying out a multi-stage immunoassay of at least one biological substance in a plurality of biological samples: method using said apparatus |
| CN201464472U (en) * | 2009-09-01 | 2010-05-12 | 郑州博赛生物技术股份有限公司 | Reaction cup holder for detection kit of fully-automatic immunoassay analyzer |
| CN201607447U (en) * | 2009-12-31 | 2010-10-13 | 上海丰汇医用仪器有限公司 | Reaction tray of full-automatic liquid-phase chemical luminescence immune analyzer |
| CN203881788U (en) * | 2014-04-01 | 2014-10-15 | 重庆科斯迈生物科技有限公司 | Chemiluminescence immune analyzer test tube rack |
| CN203881782U (en) * | 2014-04-01 | 2014-10-15 | 重庆科斯迈生物科技有限公司 | Cup position detection system of chemiluminiscence immunoassay analyzer |
| CN106290818A (en) * | 2016-08-31 | 2017-01-04 | 成都恩普生医疗科技有限公司 | A kind of reaction cup assembly |
| CN106468711A (en) * | 2016-09-07 | 2017-03-01 | 北京热景生物技术股份有限公司 | DCP sharp separation detection kit |
-
2017
- 2017-05-27 CN CN201710390260.8A patent/CN107328935B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5559002A (en) * | 1989-11-17 | 1996-09-24 | Laboratoires Biotrol | Apparatus for automatically carrying out a multi-stage immunoassay of at least one biological substance in a plurality of biological samples: method using said apparatus |
| CN201464472U (en) * | 2009-09-01 | 2010-05-12 | 郑州博赛生物技术股份有限公司 | Reaction cup holder for detection kit of fully-automatic immunoassay analyzer |
| CN201607447U (en) * | 2009-12-31 | 2010-10-13 | 上海丰汇医用仪器有限公司 | Reaction tray of full-automatic liquid-phase chemical luminescence immune analyzer |
| CN203881788U (en) * | 2014-04-01 | 2014-10-15 | 重庆科斯迈生物科技有限公司 | Chemiluminescence immune analyzer test tube rack |
| CN203881782U (en) * | 2014-04-01 | 2014-10-15 | 重庆科斯迈生物科技有限公司 | Cup position detection system of chemiluminiscence immunoassay analyzer |
| CN106290818A (en) * | 2016-08-31 | 2017-01-04 | 成都恩普生医疗科技有限公司 | A kind of reaction cup assembly |
| CN106468711A (en) * | 2016-09-07 | 2017-03-01 | 北京热景生物技术股份有限公司 | DCP sharp separation detection kit |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107328935A (en) | 2017-11-07 |
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