CN107328829A - A kind of cellular electrophysiologicalsensor detection system and method based on scanning probe - Google Patents

A kind of cellular electrophysiologicalsensor detection system and method based on scanning probe Download PDF

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Publication number
CN107328829A
CN107328829A CN201710495782.4A CN201710495782A CN107328829A CN 107328829 A CN107328829 A CN 107328829A CN 201710495782 A CN201710495782 A CN 201710495782A CN 107328829 A CN107328829 A CN 107328829A
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cell membrane
membrane sample
electric signal
ion channel
sample
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CN107328829B (en
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李灯华
许世卫
李哲敏
陈威
张超
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Agricultural Information Institute of CAAS
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis

Abstract

The invention discloses a kind of cellular electrophysiologicalsensor detection system and method based on scanning probe.The system includes:Conducting scanning probe;Micro-cantilever, the fixed conducting scanning probe, the needle point for driving conducting scanning probe is scanned and detected;Micro-cantilever motion detection apparatus and backfeed loop, connect the micro-cantilever, are detected and controlled for the motion to micro-cantilever;Piezoelectric ceramic scanatron, micro-cantilever described in grafting, for controlling micro-cantilever to drive the conducting scanning probe to be scanned and detect cell membrane sample;Terminal control and image capturing system, are imaged and are analyzed and processed for the electrophysiology information to cell membrane sample.The present invention can detect ion channel in cell membrane electrical characteristics and ion channel nanoscale three-dimensional appearance simultaneously, and not destroy sample structure and property.

Description

A kind of cellular electrophysiologicalsensor detection system and method based on scanning probe
Technical field
The present embodiments relate to cell electrical resistivity survey technology field, more particularly to a kind of cell electricity based on scanning probe are raw Manage detection system and method.
Background technology
Biological electricity is played a significant role in plant growth vital movement, and electric signal all can to many physiological activities of plant Produce material impact, the deformation of such as cell, stomatal movement, solute transport, holding cellular osmotic balance, cellular signal transduction. Iuntercellular passes through one of basic mode that electric signal conducts information is that plant information is conducted.The ion permeability of cell membrane is to crop Yield and quality has a major impact.Scientist is found that cell biological film is penetrating to ion in the research of biological electricity generation mechanism The change of property.The specific proteins that ion channel is produced by cell are constituted, and they gather together and are embedded on cell membrane, middle The hole that hydrone is occupied is formed, these holes are exactly the passage that water-soluble substances quickly passes in and out cell.The activity of ion channel To realizing that cell various functions are significant.The function of ion channel on cell membrane, except intraor extracellular can be adjusted Osmotic pressure, is also the important molecule for maintaining cell membrane potential, and cell will carry out electric signal conduction, be by ion turnover with Cause the change of film potential.At present epicyte protein electrical characteristics and ionic selective permeability are still lacked with complete set detailed Molecular mechanism of action, reason is to lack the technical method for obtaining memebrane protein three-dimensional structure, lacks and obtain cell membrane biological simultaneously The technological means of electric signal and epicyte protein high-precision configuration.
The method of tradition research cell membrane electrical characteristics and ion channel performance includes:Patch clamp technique, channel protein separation Biochemical technology, artificial membrane ion channel reconstitution technology and some physics and the chemical technologies such as purifying.Can be with using patch clamp technique Directly observe and differentiate single ionic channel electric current and its opening and closing time-histories, but can not real-time detection cell membrane nanostructured morphologies, The structural information and electrical characteristics of cell membrane nanoscale can not be obtained simultaneously.Some other channel protein is isolated and purified, artificial membrane The biochemical technology material such as ion channel reconstitution technology prepare it is numerous and diverse, and need by other technologies means detect cell membrane electrical characteristics And ion permeability.
The content of the invention
The present invention provides a kind of cellular electrophysiologicalsensor detection system and method based on scanning probe, to realize in sample surfaces While detecting nanoscale pattern, the channel current and electrology characteristic of cell membrane are obtained in real time, and do not destroy sample structure And property.
In a first aspect, the embodiments of the invention provide a kind of cellular electrophysiologicalsensor detection system based on scanning probe, this is System includes:
Conducting scanning probe, for cell membrane sample extrinsic motivated electric signal so that the cell membrane sample obtains electricity Characteristic response, the needle point of conducting scanning probe is used for the electrophysiological characteristics for detecting cell membrane sample;
Micro-cantilever, the fixed conducting scanning probe, the needle point for driving conducting scanning probe is scanned and detected;
Micro-cantilever motion detection apparatus and backfeed loop, connect the micro-cantilever, are examined for the motion to micro-cantilever Survey and control;
Piezoelectric ceramic scanatron, micro-cantilever described in grafting, for controlling micro-cantilever to drive the conducting scanning probe to thin After birth sample is scanned and detected;
Terminal control and image capturing system, connect the micro-cantilever, micro-cantilever motion detection apparatus and backfeed loop and Piezoelectric ceramic scanatron, is imaged and is analyzed and processed for the electrophysiology information to cell membrane sample.
Preferably, the system also includes sample room, and the cell membrane sample, conducting scanning probe and sample substrate are respectively positioned on The sample room;The sample room includes humidity control module, temperature control modules and medicine control module.
Preferably, the humidity control module is connected with air and water pump, can be pumped into vapor in sample room and adjust the sample The humidity of product room.
Preferably, temp-controlling element is installed inside the temperature control modules.
Preferably, the medicine control module includes delivery system
Second aspect, the embodiment of the present invention additionally provides a kind of cellular electrophysiologicalsensor detection method based on scanning probe, by System described in first aspect is performed, and this method includes:
Terminal control drives the needle point to be contacted with the cell membrane sample on sample substrate with image capturing system;
Terminal control and image capturing system, to the cell membrane sample extrinsic motivated electric signal, make institute by the needle point State cell membrane sample and obtain electrical characteristics response;
Terminal control uses cell membrane sample described in the conducting scanning probe synchronous recording outside with image capturing system Plus ion channel pattern and electric signal during excitation electric signal.
Preferably, the cell membrane sample extrinsic motivated electric signal is specifically included by the needle point:Pass through the pin Point produces membrance current signal to the cell membrane sample extrinsic motivated voltage, the now ion channel of the cell membrane sample;Or Exciting current is injected to the cell membrane sample by the needle point, now the ion channel generation film of the cell membrane sample is inclined Press signal.
Preferably, using cell membrane sample described in the conducting scanning probe synchronous recording in extrinsic motivated electric signal Ion channel pattern and electric signal are specifically included:The cell membrane sample is detected using the conducting scanning probe synchronous scanning to exist Ion channel pattern and each ion channel electric signal during extrinsic motivated electric signal;
It is logical using ion of the cell membrane sample in extrinsic motivated electric signal described in the conducting scanning probe synchronous recording After road pattern and electric signal, in addition to:According to the ion channel shape appearance figure of ion channel morphogenesis cell membrane sample, according to The electric signal distribution map of each ion channel electric signal cellulation membrane sample.
Preferably, terminal control uses cell membrane sample described in the conducting scanning probe synchronous recording with image capturing system After ion channel pattern and electric signal of the product in extrinsic motivated electric signal, in addition to:Terminal control and image capturing system According to the ion channel pattern and electric signal of the cell membrane sample, drive the conductive pinpoint be positioned at cell membrane sample from Subchannel is pinpointed, and changes the extrinsic motivated electric signal, the local electric signal of the ion channel fixed point of record cell membrane sample.
Preferably, terminal control uses the scanning probe records cell membrane sample in extrinsic motivated with image capturing system After ion channel pattern and electric signal during electric signal, in addition to:
Terminal control changes cell membrane sample environment with image capturing system, and record cell membrane sample is in extrinsic motivated telecommunications Number when ion channel pattern and electric signal and cell membrane sample environment relation;Or
Terminal control and ion channel pattern and electric signal of the image capturing system according to the cell membrane sample, drive institute The single ion channel that conductive pinpoint is positioned at cell membrane sample is stated, changes cell membrane sample environment, using scanning probe observation The single ion channel change in electric of cell membrane sample and the relation of cell membrane sample environment.The present invention provide based on scanning The system and method for the physiological detection cell membrane electrical characteristics and ion channel of probe, directly can detect nanometer chi in sample surfaces While spending pattern, the channel current and electrology characteristic of cell membrane are obtained in real time, and do not destroy sample structure and property.
Brief description of the drawings
Fig. 1 is a kind of cellular electrophysiologicalsensor detection system structural representation based on scanning probe in the embodiment of the present invention one Figure;
Fig. 2 is sample room in another cellular electrophysiologicalsensor detection system based on scanning probe in the embodiment of the present invention one Interior structural representation;
Fig. 3 is a kind of cellular electrophysiologicalsensor detection method flow chart based on scanning probe in the embodiment of the present invention three;
Fig. 4 is a kind of cellular electrophysiologicalsensor detection method flow chart based on scanning probe in the embodiment of the present invention four;
Fig. 5 is a kind of cellular electrophysiologicalsensor detection method flow chart based on scanning probe in the embodiment of the present invention five.
Embodiment
The present invention is described in further detail with reference to the accompanying drawings and examples.It is understood that this place is retouched The specific embodiment stated is used only for explaining the present invention, rather than limitation of the invention.It also should be noted that, in order to just Part related to the present invention rather than entire infrastructure are illustrate only in description, accompanying drawing.
Embodiment one
Fig. 1-2 is refer to, Fig. 1-2 is a kind of cellular electrophysiologicalsensor based on scanning probe that first embodiment of the invention is provided The structural representation of detection system.As depicted in figs. 1 and 2, the system includes:
Conducting scanning probe 2, conducting scanning probe 2 is used for the extrinsic motivated electric signal of cell membrane sample 6 so that described thin After birth sample 6 obtains electrical characteristics response, and the needle point of conducting scanning probe is used for the electrophysiological characteristics for detecting cell membrane sample 6;Institute Cell membrane sample 6 is stated in the sample substrate 7;
Micro-cantilever 3, the fixed conducting scanning probe 2, the needle point for driving conducting scanning probe 2 is scanned and visited Survey;
Micro-cantilever motion detection apparatus and backfeed loop 4, connect the micro-cantilever 3, for the motion progress to micro-cantilever 3 Detection and control;
Piezoelectric ceramic scanatron 5, micro-cantilever 3 described in grafting, for controlling micro-cantilever 3 to drive the conducting scanning probe 2 Cell membrane sample 6 is scanned and detected;
Terminal control and image capturing system 1, connect the micro-cantilever 3, micro-cantilever motion detection apparatus and backfeed loop 4 With piezoelectric ceramic scanatron 5, it is imaged and is analyzed and processed for the electrophysiology information to cell membrane sample.
Specifically, the body construction of the needle point of conducting scanning probe 2 is silica or silicon nitride material, needle point coating is led Electric layer, conductive layer is golden film, chromium, titanium, platinoiridita laminated film etc..
Conducting scanning probe 2 is moved under control of the terminal control with image capturing system 1, is contacted with cell membrane sample, To cell membrane sample extrinsic motivated electric signal so that the cell membrane sample obtains photoelectric characteristic response, records cell membrane sample Ion channel pattern and electric signal under extrinsic motivated electric signal, and the ion channel pattern and electrical signal information are transmitted To terminal control and image capturing system 1, image capturing system 1 is imaged and carried out to the electrophysiology information of cell membrane sample Analyzing and processing.
The cellular electrophysiologicalsensor detection method based on scanning probe of the present embodiment, ion channel in cell membrane electricity can be detected simultaneously Characteristic and ion channel nanoscale three-dimensional appearance, and sample structure and property are not destroyed.
On the basis of above-mentioned technical proposal, the system also includes sample room 8, and the cell membrane sample 6, conductive scanning are visited Pin 2 and sample substrate 7 are respectively positioned on the sample room 8;The sample room 8 includes humidity control module, temperature control modules and medicine Thing control module.The humidity control module, temperature control modules and medicine control module connect respectively the terminal control with Image capturing system 1.
Specifically, the humidity control module is connected with air and water pump, can be pumped into vapor in sample room and adjust the sample The humidity of product room.The sample room 7 is connected with air and water pump, and vapor can be pumped into the sample room 7 by the air and water pump.The sample Product room 7 is the sealing device with moisture outlet and moisture ingress, and moisture ingress is connected with air and water pump 3, and air and water pump 3 can be by vapor It is pumped into sample room 7 and adjusts humidity, keeps the bioactivity of cell membrane sample to be measured.
Temp-controlling element is installed, temp-controlling element can be semiconductor refrigerating element, control cell inside the temperature control modules Membrane sample temperature is maintained in less than 10 degree of low temperature environment, keeps the bioactivity of cell membrane sample to be measured.
The medicine control module includes delivery system, can apply specific medicine to cell membrane sample, observe cell Electro physiology performance of the membrane sample under different pharmaceutical environment.
By controlling sample room sample environment, including temperature, humidity, medicine etc., to observe single channel opener and closing Curent change, the work(such as current amplitude distribution, open probability, open life-span distribution that various ion channels are opened can be directly obtained Energy parameter, and analyze the relation between they and film potential, ion concentration etc..
Embodiment two
Fig. 3 is a kind of flow for cellular electrophysiologicalsensor detection method based on scanning probe that the embodiment of the present invention two is provided Figure, the present embodiment be applicable to cell electrical characteristics and ion channel detection situation, for example, rice leaf cell electrology characteristic and Ion channel is detected, wheat root cell electrology characteristic and ion channel detection etc., and this method can be as described in embodiment one Performed based on the cellular electrophysiologicalsensor detection system of scanning probe.As shown in figure 3, this method includes:
Step S110, terminal control and image capturing system driving conducting scanning probe needle point with positioned at sample substrate it On cell membrane sample contact.
Wherein, cell membrane sample is located on sample substrate, and scanning probe is located above cell membrane, driving scanning probe A conductive pinpoint side contacts relative with sample substrate with cell membrane sample.
Step S120, terminal control and image capturing system are by the needle point to cell membrane sample extrinsic motivated electricity Signal so that the cell membrane sample obtains electrical characteristics response.
Wherein, by the conductive pinpoint to the cell membrane sample extrinsic motivated electric signal so that the cell membrane sample Product obtain electrical characteristics response, and the opened/closed degree that the ion channel of cell membrane sample is logical changes, and causes ion channel shape Looks and electric signal change.
The cell membrane sample extrinsic motivated electric signal is specifically included by the conductive pinpoint:Pass through the conductive pin Point produces membrance current signal to the cell membrane sample extrinsic motivated voltage, the now ion channel of the cell membrane sample;Or Exciting current is injected to the cell membrane sample by the conductive pinpoint, now the ion channel of the cell membrane sample is produced Film bias voltage signal.
For example, by the conductive pinpoint to the cell membrane sample extrinsic motivated voltage so that the cell membrane sample Electrical characteristics response is obtained, extrinsic motivated voltage can arrive+10V for -10V, apply alive resolution ratio for mV grades.Normal plants are thin Born of the same parents, when applying 100mV biass, ion channel current is 50pA, and passes through Na+ channel blocker tetraodotoxins to cell (TTX) after handling, when applying 100mV biass, electric current is 5pA.Xenopus leavis oocytes are applied with 100mV biass, ion channel Electric current reaches 1uA.The selection of extrinsic motivated voltage swing is led to depending on the ion of cell membrane sample resistance sizes and ion channel Permeability, those skilled in the art can be selected as needed.The present invention is not especially limited to driving voltage.
Exciting current is injected to the cell membrane sample by the conductive pinpoint so that the cell membrane sample obtains electricity Characteristic response, exciting current can be 1pA to 1nA.It is big that the selection of extrinsic motivated size of current depends on cell membrane sample electric capacity The ion permeability of small and ion channel, those skilled in the art can be selected as needed.The present invention is to exciting current It is not especially limited.
Step S130, terminal control and image capturing system use cell membrane described in the conducting scanning probe synchronous recording Ion channel pattern and electric signal of the sample in extrinsic motivated electric signal.
Wherein, using ion channel shape of the cell membrane sample in extrinsic motivated electric signal described in the scanning probe records Looks and electric signal are specifically included:The cell membrane sample is detected in extrinsic motivated electric signal using the scanning probe scanning Ion channel pattern, ion channel electricity of the cell membrane sample in extrinsic motivated electric signal is detected using the scanning probe Signal.
In addition, the electric signal in order to be able to more accurately record ion channel, can also pass through the electric signal of ion channel Preamplifier amplification is recorded again.
Preferably, using ion channel of the cell membrane sample in extrinsic motivated electric signal described in the scanning probe records After pattern and electric signal, in addition to:According to the ion channel shape appearance figure of ion channel morphogenesis cell membrane sample, according to each The electric signal distribution map of ion channel electric signal cellulation membrane sample.
The cellular electrophysiologicalsensor detection method based on scanning probe of the present embodiment, ion channel in cell membrane electricity can be detected simultaneously Characteristic and ion channel nanoscale three-dimensional appearance, and sample structure and property are not destroyed.
Embodiment three
Fig. 4 is refer to, Fig. 4 is a kind of cellular electrophysiologicalsensor detection side based on scanning probe that the embodiment of the present invention three is provided The flow chart of method.Improvement is optimized based on above-described embodiment scheme in the present embodiment, in particular, provides fixed point detection Concrete scheme.As shown in figure 4, this method includes:
Step S210, terminal control and image capturing system driving conducting scanning probe needle point with positioned at sample substrate it On cell membrane sample contact.
Step S220, terminal control and image capturing system are by the needle point to cell membrane sample extrinsic motivated electricity Signal so that the cell membrane sample obtains electrical characteristics response,.
Step S230, terminal control and image capturing system record the cell membrane sample using the conducting scanning probe Ion channel pattern and electric signal in extrinsic motivated electric signal.
Step S240, ion channel pattern and electric signal according to the cell membrane sample, drive the conductive pinpoint to determine Ion channel positioned at cell membrane sample is pinpointed, and changes the extrinsic motivated electric signal, using scanning probe records cell membrane sample The local electric signal of the ion channel fixed point of product.
Wherein, change the extrinsic motivated electric signal, pinpointed using the ion channel of scanning probe detection cell membrane sample Pattern and local electric signal under different extrinsic motivated electric signals, so that according under different extrinsic motivated electric signals Local electric signal, obtains the ion channel electrical characteristics curve of cell membrane sample.
For example, taking curved scanning mode, applying a series of setting speed to cell membrane sample by conductive pinpoint changes Bias, then carries out channel current detection to cell membrane sample, can obtain memebrane protein ion channel current voltage curve.
The present embodiment uses the detection mode that contact type scanning and fixed point detection are combined.Contact type scanning, is utilized Conductive pinpoint scanning probe cell membrane sample ion channel pattern and electric signal;Fixed point detection, according to ion channel pattern and electricity Signal, is positioned at ion channel in the sample of scanning using needle point and pinpoints, the local electric current of ion channel is measured and Imaging.So as to directly sample surfaces detect nanoscale pattern while, in real time obtain cell membrane channel current and Electrology characteristic, and sample structure and property are not destroyed.
Example IV
Fig. 5 is refer to, Fig. 5 is a kind of cellular electrophysiologicalsensor detection side based on scanning probe that the embodiment of the present invention four is provided The flow chart of method.Improvement is optimized based on above-described embodiment scheme in the present embodiment, in particular, provides change sample Environment is scanned the concrete scheme of detection or fixed point detection.As shown in figure 5, this method includes:
Step S310, terminal control and image capturing system driving conducting scanning probe needle point with positioned at sample substrate it On cell membrane sample contact.
Step S320, terminal control and image capturing system are by the needle point to cell membrane sample extrinsic motivated electricity Signal so that the cell membrane sample obtains electrical characteristics response.
Step S330, terminal control and image capturing system record the cell membrane sample using the conducting scanning probe Ion channel pattern and electric signal in extrinsic motivated electric signal.
Step S340, terminal control and image capturing system change cell membrane sample environment, and record cell membrane sample is outside Plus ion channel pattern and the relation of electric signal and cell membrane sample environment during excitation electric signal;Or
Terminal control and ion channel pattern and electric signal of the image capturing system according to the cell membrane sample, drive institute The single ion channel that needle point is positioned at cell membrane sample is stated, changes cell membrane sample environment, using scanning probe observation cell The single ion channel change in electric of membrane sample and the relation of cell membrane sample environment.
Wherein, changing cell membrane sample environment includes medicine, humidity or temperature etc., on the one hand, scanned by conductive pinpoint Ion under the conditions of the photoelectric respone change in electric of detection cell membrane sample under difficult environmental conditions, generation varying environment leads to Road feature image and photoelectric respone electric signal image, what is now obtained is cell membrane sample entirety or the ion channel in some region Feature image and photoelectric respone electric signal image;On the other hand, the conductive pinpoint is driven to be positioned at the single of cell membrane sample Ion channel, to observe the change in electric of the photoelectric respone of single ion channel under difficult environmental conditions, is now obtained It is the single ion channel photoelectric respone change in electric of cell membrane sample.
The electric signal opened by each ion channel, can directly obtain electric signal amplitude distribution that each ion channel opens, The function parameter such as probability and open life-span distribution is opened, on this basis acceptable and analytic function parameter and film potential, ion Relation between concentration etc..
The present embodiment uses the detection mode that contact type scanning and fixed point detection are combined, can be directly in sample table While face detects nanoscale pattern, the channel current and electrology characteristic of cell membrane are obtained in real time, and do not destroy sample knot Structure and property.
The said goods can perform the method that any embodiment of the present invention is provided, and possess the corresponding functional module of execution method And beneficial effect.
Note, above are only presently preferred embodiments of the present invention and institute's application technology principle.It will be appreciated by those skilled in the art that The invention is not restricted to specific embodiment described here, can carry out for a person skilled in the art it is various it is obvious change, Readjust and substitute without departing from protection scope of the present invention.Therefore, although the present invention is carried out by above example It is described in further detail, but the present invention is not limited only to above example, without departing from the inventive concept, also Other more equivalent embodiments can be included, and the scope of the present invention is determined by scope of the appended claims.

Claims (10)

1. a kind of cellular electrophysiologicalsensor detection system based on scanning probe, it is characterised in that the system includes:
Conducting scanning probe, for cell membrane sample extrinsic motivated electric signal so that the cell membrane sample obtains electrical characteristics Response, the needle point of conducting scanning probe is used for the electrophysiological characteristics for detecting cell membrane sample;
Micro-cantilever, the fixed conducting scanning probe, the needle point for driving conducting scanning probe is scanned and detected;
Micro-cantilever motion detection apparatus and backfeed loop, connect the micro-cantilever, for the motion to micro-cantilever carry out detection and Control;
Piezoelectric ceramic scanatron, micro-cantilever described in grafting, for controlling micro-cantilever to drive the conducting scanning probe to cell membrane Sample is scanned and detected;
Terminal control and image capturing system, connect the micro-cantilever, micro-cantilever motion detection apparatus and backfeed loop and piezoelectricity Ceramic flying-spot tube, is imaged and is analyzed and processed for the electrophysiology information to cell membrane sample.
2. system according to claim 1, it is characterised in that the system also includes sample room, the cell membrane sample, lead Electric scanning probe and sample substrate are respectively positioned on the sample room;The sample room includes humidity control module, temperature control modules With medicine control module.
3. system according to claim 2, it is characterised in that the humidity control module is connected with air and water pump, can be by water Steam is pumped into the humidity that the sample room is adjusted in sample room.
4. system according to claim 2, it is characterised in that temp-controlling element is installed inside the temperature control modules.
5. system according to claim 2, it is characterised in that the medicine control module includes delivery system.
6. a kind of cellular electrophysiologicalsensor detection method based on scanning probe, it is characterised in that this method is any by claim 1-5 System described in is performed, including:
Terminal control drives the needle point to be contacted with the cell membrane sample on sample substrate with image capturing system;
Terminal control and image capturing system, to the cell membrane sample extrinsic motivated electric signal, are made described thin by the needle point After birth sample obtains electrical characteristics response;
Terminal control is swashed with image capturing system using cell membrane sample described in the conducting scanning probe synchronous recording additional Encourage the ion channel pattern and electric signal during electric signal.
7. method according to claim 6, it is characterised in that by the needle point to the cell membrane sample extrinsic motivated Electric signal is specifically included:By the needle point to the cell membrane sample extrinsic motivated voltage, the now cell membrane sample Ion channel produces membrance current signal;Or exciting current is injected to the cell membrane sample by the needle point, it is now described thin The ion channel of after birth sample produces film bias voltage signal.
8. method according to claim 6, it is characterised in that using cell described in the conducting scanning probe synchronous recording Ion channel pattern and electric signal of the membrane sample in extrinsic motivated electric signal are specifically included:It is same using the conducting scanning probe Walk ion channel pattern and each ion channel electric signal of the cell membrane sample in extrinsic motivated electric signal described in scanning probe;
Using ion channel shape of the cell membrane sample in extrinsic motivated electric signal described in the conducting scanning probe synchronous recording After looks and electric signal, in addition to:According to the ion channel shape appearance figure of ion channel morphogenesis cell membrane sample, according to respectively from The electric signal distribution map of subchannel electric signal cellulation membrane sample.
9. method according to claim 6, it is characterised in that terminal control is swept with image capturing system using the conduction Retouch after ion channel pattern and electric signal of the cell membrane sample described in probe synchronous recording in extrinsic motivated electric signal, also wrap Include:Terminal control and image capturing system are led described in driving according to the ion channel pattern and electric signal of the cell membrane sample Acusector point is positioned at the ion channel fixed point of cell membrane sample, changes the extrinsic motivated electric signal, record cell membrane sample The local electric signal of ion channel fixed point.
10. method according to claim 6, it is characterised in that terminal control uses the scanning with image capturing system After ion channel pattern and electric signal of the probe records cell membrane sample in extrinsic motivated electric signal, in addition to:
Terminal control changes cell membrane sample environment with image capturing system, and record cell membrane sample is in extrinsic motivated electric signal Ion channel pattern and electric signal and cell membrane sample environment relation;Or
Terminal control and image capturing system are led described in driving according to the ion channel pattern and electric signal of the cell membrane sample Acusector point is positioned at the single ion channel of cell membrane sample, changes cell membrane sample environment, using scanning probe observation cell The single ion channel change in electric of membrane sample and the relation of cell membrane sample environment.
CN201710495782.4A 2017-06-26 2017-06-26 A kind of cellular electrophysiologicalsensor detection system and method based on scanning probe Active CN107328829B (en)

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