CN107299121A - The amino acid fermentation method of industrial phosphoric acid salt is replaced using corn syrup hydrolyzate - Google Patents
The amino acid fermentation method of industrial phosphoric acid salt is replaced using corn syrup hydrolyzate Download PDFInfo
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- CN107299121A CN107299121A CN201710739285.4A CN201710739285A CN107299121A CN 107299121 A CN107299121 A CN 107299121A CN 201710739285 A CN201710739285 A CN 201710739285A CN 107299121 A CN107299121 A CN 107299121A
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Abstract
The amino acid fermentation method that corn syrup hydrolyzate replaces industrial phosphoric acid salt is the present invention relates to the use of, the fermentation process for being directly used in amino acid for producing the corn syrup hydrolyzate of needs instead of phosphorus sources such as corn steep liquor and phosphoric acid is met using the free phosphorus content of phytic acid enzyme hydrolyzed corn slurry generation.The invention provides one kind using corn steep liquor as raw material, the corn syrup hydrolyzate produced after phytic acid enzyme hydrolysis directly uses corn steep liquor and adds phosphoric acid as the mode of phosphorus source during substituting usual amino acid production by ferment method, production target is basically identical with normal fermentation, and cost needed for all phytases is far below can reach the phosphoric acid cost of equivalent effect, so with low cost, this method is while normal production is ensured, amino acid can effectively be reduced and integrate production cost, Business Economic Benefit is directly provided, suitable for popularizing in an all-round way in production.
Description
Technical field
Industrial phosphoric acid salt is replaced the invention belongs to bio-fermentation engineering field, more particularly to using corn syrup hydrolyzate
Amino acid fermentation method.
Background technology
General, amino acid fermentation needs carbon source, nitrogen source, phosphorus source and other micro- and growth factors etc..Wherein phosphorus
It is the neccessary composition of nucleic acid and protein, is also the important component of cell membrane（Phosphatide）, phosphorus plays an important role in the cell.For
The growth of microorganism is maintained, phosphorus is often added in culture medium in the form of phosphate, the phosphate commonly used in industrial production has
K3PO4·3H2O、K3PO4、Na2HPO4·12H2O、NaH2PO4·2H2O etc..
Corn steep liquor be wet processing manufacture starch during soaking corn produce soak formed through being concentrated under reduced pressure, wherein
Containing nutriments such as substantial amounts of soluble protein, polypeptide, soluble sugar, several amino acids, vitamin, inorganic salts, micro-
It is main in biofermentation production that nitrogen source and growth factor are provided and are widely used, especially in the production of Thermo-sensitive glutamic acid fermentation
Nitrogen source and biotin etc. can be provided for it, have large effect to the production acid and conversion ratio of fermentation.Corn steep liquor is except that can be hair
Ferment production provides nitrogen source and growth factor, also containing to microorganism growth and the important trace element that ferments, such as phosphorus, iron,
Magnesium, zinc etc..
Containing abundant P elements in corn steep liquor, but it is mostly to exist in the phytic acid form of organic, the change of phytic acid
Scientific name is referred to as inositol hexaphosphate, is the main existence form of vegetable seeds mysoinositol and phosphoric acid, in alimentary crops such as cereal
Content is up to 1% ~ 3% in seed, accounts for the 40% ~ 70% of the phosphorous total amount of plant.During corn soaking, most phytic acid quilt
Dissolution, but it is difficult to be utilized to lack the P elements that can be decomposed in the enzyme of phytic acid, phytic acid in most microorganism, thus make
Into the loss and waste of P elements.Phytic acid is a kind of also ANFs simultaneously, unprocessed to influence a variety of nutriments
Utilize, phosphate group thereon is in elecrtonegativity, can be with Ca with very strong complexing power2+、Mg2+、Zn2+、Cu2+、Mn2+、Fe2+And K+Stable compound is chelated into Deng metal ion and protein, insoluble salt is formed, so as to influence microorganism to a variety of nutrition
The utilization of material.
If the organophosphor in dregs of rice class culture medium can effectively be hydrolyzed in fermentation process, so as to be met
Free phosphorus needed for microbial fermentation, will not only improve the utilization rate of nutriment in corn steep liquor, can also avoid additional phosphorus source,
Row's phosphorus amount in nature is decreased simultaneously, is conducive to environmental protection.
Phytase can be catalyzed phytic acid and be decomposed into phosphoric acid and inositol, and it can hydrolyze phosphate group from phytic acid, will
The phosphorus of organic is transformed into the Phos for the free state that microorganism can directly utilize, while also destroying phytic acid to Ca2+、Mg2 +、Zn2+The affinity strong etc. other mineral elements, so as to improve utilization rate of the microorganism to a variety of nutriments.
Therefore bio-fermentation engineering is applied herein, and phytase is used for corn steep liquor hydrolysed ferment so as to by corn steep liquor
The release of phosphate group, replacing corn steep liquor progress to feed intake using corn syrup hydrolyzate makes no longer to need to add phosphoric acid in fermentation process
Phosphorus source is supplemented, the unfavorable factor in some production processes can be so eliminated, raw material advantage can be more utilized, reduce the wasting of resources,
The production cost of enterprise is reduced, increases company interest.
The content of the invention
The purpose of the present invention be in view of the deficiencies of the prior art there is provided utilization corn syrup hydrolyzate replace industrial phosphorus
The amino acid fermentation method of hydrochlorate, this method is avoided that in fermentation production process extra supplement phosphorus source, reduces production cost.
The present invention is to be achieved through the following technical solutions：
The amino acid fermentation method of industrial phosphoric acid salt is replaced using corn syrup hydrolyzate, by adding phytase system in corn steep liquor
Make corn syrup hydrolyzate, improve the content of free phosphorus phosphorus in corn steep liquor, it is to avoid extra supplement phosphorus during fermenting and producing amino acid
Source.Comprise the following steps that：
（1）Corn steep liquor is taken, pH is adjusted, phytase is added, stirs；
（2）Corn steep liquor is placed under 50-70 DEG C of temperature conditionss, phytase is added, stirs, produce hydrolyzate；
（3）By step（2）Gained hydrolyzate is used for fermenting and producing amino acid.
Preferred scheme is, step（1）Adjust pH to 3.5-5.5.
Preferred scheme is, step（1）The consumption of added phytase is the 0.05%-0.1% of corn steep liquor quality.
Preferred scheme is, step（2）Hydrolysis 6-9 hours.
The advantage and good effect that the present invention is obtained be：
1st, the invention provides one kind using corn steep liquor as raw material, the corn syrup hydrolyzate produced after phytic acid enzyme hydrolysis substitutes usual
Directly use corn steep liquor during amino acid production by ferment method and add phosphoric acid as the mode of phosphorus source, production target and normal fermentation
It is basically identical, reduce the wasting of resources, and all phytases required cost far below the phosphoric acid cost of equivalent effect can be reached,
Meet the energy-saving and emission-reduction theory advocated now；
2nd, the inventive method can effectively reduce amino acid and integrate production cost, directly provide enterprise while normal production is ensured
Industry economic benefit, suitable for popularizing in an all-round way in production.
, this method whole process it is easy to operate, remarkable in economical benefits, be fully compatible for carry out industrialized production.
Embodiment
With reference to embodiment, the present invention is described in further detail, but the implementation of the present invention is not limited to this,
Protection scope of the present invention can not be limited with following embodiments.Experimental method used in following embodiments is such as without special
Illustrate, be conventional method；Used material, reagent etc., unless otherwise specified, are commercially obtained.
Embodiment 1
Replace using after phytic acid enzyme hydrolysis in the amino acid fermentation method of industrial phosphoric acid salt, fermentation process using corn syrup hydrolyzate
Corn syrup hydrolyzate fed intake instead of corn steep liquor with phosphoric acid.Comprise the following steps that：
（1）Corn steep liquor 50g is taken, pH4.0 is adjusted, 0.025g phytase 1 is added, stirs；
（2）Hydrolyzed 6 hours under conditions of being placed in 50 DEG C, you can obtain corn syrup hydrolyzate；
（3）By step（2）Gained hydrolyzate is used for fermenting and producing glutamic acid.
Wherein phytase 1 is phytase 340, enzyme activity 30000u, purchased from Weifang KDN Biotechnology Co., Ltd..
Embodiment 2
Replace using after phytic acid enzyme hydrolysis in the amino acid fermentation method of industrial phosphoric acid salt, fermentation process using corn syrup hydrolyzate
Corn syrup hydrolyzate fed intake instead of corn steep liquor with phosphoric acid.
（1）Corn steep liquor 50g is taken, pH5.0 is adjusted, 0.04g phytase 2 is added, stirs；
（2）Hydrolyzed 9 hours under conditions of being placed in 65 DEG C, you can obtain corn syrup hydrolyzate；
（3）By step（2）Gained hydrolyzate is used for fermenting and producing threonine.
Wherein phytase 2 is the U.S. phosphorus of good fortune, enzyme activity 5000u, purchased from Weifang KDN Biotechnology Co., Ltd.
Embodiment 3
Replace using after phytic acid enzyme hydrolysis in the amino acid fermentation method of industrial phosphoric acid salt, fermentation process using corn syrup hydrolyzate
Corn syrup hydrolyzate fed intake instead of corn steep liquor with phosphoric acid.
（1）Corn steep liquor 50g is taken, pH4.0 is adjusted, 0.025g phytase 1 is added, stirs；
（2）Hydrolyzed 6 hours under conditions of being placed in 50 DEG C, you can obtain corn syrup hydrolyzate；
（3）By step（2）Gained hydrolyzate is used for fermenting and producing lysine.
Wherein phytase 1 is phytase 340, enzyme activity 30000u, purchased from Weifang KDN Biotechnology Co., Ltd..
Test example 1
With the corn steep liquor and corn syrup hydrolyzate in embodiment 1, chemical detection analysis is carried out：
After testing, the phosphorus content that dissociates before hydrolyzing in corn steep liquor is 3.25 mg/g, and the phosphorus content that dissociates after hydrolysis is 11.52mg/g, trip
It is 8.27 mg/g from the phosphorus amount of having a net increase of, that is, free phosphorus increment is 8.27Kg in 1t corn steep liquors after hydrolyzing；Fermentation tank list in actual production
The corn steep liquor addition of tank is about 13 ~ 16t, and 85% phosphoric acid inventory is 420Kg, is converted to free phosphorus content for 112.85Kg；
Single batch fermentation production can be met to P elements needs by being computed 13.65t hydrolysed corn slurry.
Wherein described 85% phosphoric acid price is：5080 yuan/ton；The 420Kg85% phosphoric acid price is：2133.65 it is first；It is described
The price of phytase 1 is：28 yuan/Kg；The hydrolysis 13.65t corn steep liquor requirement enzymes are：6.825 Kg；The single tank consume enzyme into
Originally it is：191.1 it is first；The power consumptions such as the increased stirring of the hydrolytic process, heating are about：175 yuan.
It is computed by data above with result, the hydrolysed corn of phytase 1 slurry increase cost price is about 366.1 yuan, and straight
The cost for connecing addition phosphoric acid is about 2133.65 yuan, and can save single batch fermentation cost through the hydrolysed corn of phytase 1 slurry is about
1767.55 yuan, there are 4 tank outputs daily in actual production, the second phase puts into production after operation, there will be 8 tank outputs daily, by 1 year
Calculate within 365 days, annual phosphorus source input is cost-effective about：516.13 ten thousand yuan/year.
Table 1 is that fermentation process of the present invention and the technical indicator of normal fermentation method are contrasted
| Index | Acid production rate（g/L） | Fermentation period h | Phosphorus content (mg/g) | Economic benefit（Ten thousand yuan/year） |
| The present invention | 195 | 28-32 | 0.05 | 513.13 |
| Known technology | 183 | 28-32 | 22.5 |
The fermentation process of the present invention is consistent with the technical indicator of normal fermentation method it can be seen from above mill trial results,
And P elements are practically free of in disabled liquid, pollution of the phosphorus to environment are reduced, while also achieving huge economic benefit.
Test example 2
With the corn steep liquor and corn syrup hydrolyzate in embodiment 2, chemical detection analysis is carried out：
After testing, the phosphorus content that dissociates before hydrolyzing in corn steep liquor is 3.12 mg/g, and the phosphorus content that dissociates after hydrolysis is 10.97mg/g, trip
It is about 7.85 mg/g from the phosphorus amount of having a net increase of, that is, free phosphorus increment is 7.85 Kg in 1t corn steep liquors after hydrolyzing；Fermented in actual production
The corn steep liquor addition of tank list tank is about 13-16t, and 85% phosphoric acid inventory is 420Kg, and being converted to free phosphorus content is
112.85Kg；Single batch fermentation production can be met to P elements needs by being computed 14.37t hydrolysed corn slurry.Wherein described 85%
Phosphoric acid price be：5080 yuan/ton；The 420Kg85% phosphoric acid price is：2133.65 it is first；The price of the phytase 2 is：
15 yuan/Kg；The hydrolysis 14.37t corn steep liquor requirement enzymes are：11.496 Kg；Single tank consumes enzyme cost：172.44 it is first；Institute
Stating the power consumptions such as the increased stirring of hydrolytic process, heating is about：195 yuan.
It is computed by data above with result, the hydrolysed corn of phytase 1 slurry increase cost price is about 367.44 yuan, and straight
The cost for connecing addition phosphoric acid is about 2133.65 yuan, and can save single batch fermentation cost through the hydrolysed corn of phytase 1 slurry is about
1766.21 yuan, there are 4 tank outputs daily in actual production, the second phase puts into production after operation, there will be 8 tank outputs daily, by 1 year
Calculate within 365 days, annual phosphorus source input is cost-effective about：515.73 ten thousand yuan/year.
Table two is that fermentation process of the present invention and the technical indicator of normal fermentation method are contrasted
| Index | Acid production rate（g/L） | Fermentation period h | Phosphorus content (mg/g) | Economic benefit（Ten thousand yuan/year） |
| The present invention | 148 | 28-32 | 0.055 | 515.73 |
| Known technology | 130 | 28-32 | 22.5 |
The fermentation process of the present invention is consistent with the technical indicator of normal fermentation method it can be seen from above mill trial results,
And P elements are practically free of in disabled liquid, pollution of the phosphorus to environment are reduced, while also achieving huge economic benefit.
Test example 3
After testing, the phosphorus content that dissociates before hydrolyzing in corn steep liquor is 3.18 mg/g, and the phosphorus content that dissociates after hydrolysis is 11.42mg/g, trip
It is 8.24 mg/g from the phosphorus amount of having a net increase of, that is, free phosphorus increment is 8.24Kg in 1t corn steep liquors after hydrolyzing；Fermentation tank list in actual production
The corn steep liquor addition of tank is about 7 ~ 10t, and 85% phosphoric acid inventory is 210Kg, is converted to free phosphorus content for 66.46Kg；Through
Single batch fermentation production can be met to P elements needs by calculating 8.04t hydrolysed corn slurry.
Wherein described 85% phosphoric acid price is：5080 yuan/ton；The 210Kg85% phosphoric acid price is：1066.8 it is first；It is described
The price of phytase 1 is：28 yuan/Kg；The hydrolysis 8.06t corn steep liquor requirement enzymes are：4.03Kg；Single tank consumption enzyme cost
For：112.84 it is first；The power consumptions such as the increased stirring of the hydrolytic process, heating are about：145 yuan.
It is computed by data above with result, the hydrolysed corn of phytase 1 slurry increase cost price is about 257.84 yuan, and straight
The cost for connecing addition phosphoric acid is about 1066.8 yuan, and can save single batch fermentation cost through the hydrolysed corn of phytase 1 slurry is about
808.96 yuan, there are 2 tank outputs daily in actual production, calculated by 365 days 1 year, annual phosphorus source input is cost-effective about
59.05 ten thousand yuan/year.
Table three is that fermentation process of the present invention and the technical indicator of normal fermentation method are contrasted
| Index | Acid production rate（g/L） | Fermentation period h | Phosphorus content (mg/g) | Economic benefit（Ten thousand yuan/year） |
| The present invention | 8 | 28-32 | 0.055 | 59.05 |
| Known technology | 8 | 28-32 | 17.85 |
The fermentation process of the present invention is consistent with the technical indicator of normal fermentation method it can be seen from above mill trial results,
And P elements are practically free of in disabled liquid, pollution of the phosphorus to environment are reduced, while also achieving huge economic benefit.
In summary, starched with phytic acid enzyme hydrolyzed corn has more obvious advantage for amino acid fermentation, it was demonstrated that the hair
Bright popularized in an all-round way in fermenting corn steep liquor production amino acid technique productions is feasible.
Claims (4)
1. the amino acid fermentation method of industrial phosphoric acid salt is replaced using corn syrup hydrolyzate, it is characterised in that this method utilizes jade
Rice ＆ peanut milk hydrolyzate is the phosphorus source needed for amino acid fermentation nutrient solution provides production bacterium, and specific preparation process is as follows：
（1）Corn steep liquor is taken, pH is adjusted, stirs；
（2）Corn steep liquor is placed under 50-70 DEG C of temperature conditionss, phytase is added, stirs, produce hydrolyzate；
（3）By step（2）Gained hydrolyzate is used for amino acid fermentation after inactivation.
2. the amino acid fermentation method of industrial phosphoric acid salt, its feature are replaced using corn syrup hydrolyzate as claimed in claim 1
It is, step（1）Adjust pH to 3.5-5.5.
3. the amino acid fermentation method of industrial phosphoric acid salt, its feature are replaced using corn syrup hydrolyzate as claimed in claim 1
It is, step（1）The consumption of phytase is the 0.05%-0.1% of corn steep liquor quality.
4. the amino acid fermentation method of industrial phosphoric acid salt, its feature are replaced using corn syrup hydrolyzate as claimed in claim 1
It is, step（2）Hydrolysis 6-9 hours.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108949864A (en) * | 2018-08-07 | 2018-12-07 | 梁山菱花生物科技有限公司 | The preparation method and application of glutamic acid fermentation sugar |
| CN111606756A (en) * | 2020-06-29 | 2020-09-01 | 黄河三角洲京博化工研究院有限公司 | Method for producing liquid fertilizer by using corn steep liquor |
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| EP2733209A2 (en) * | 2007-02-07 | 2014-05-21 | Danisco US, Inc., Genencor Division | Variant Buttiauxella sp.phytases having altered properties |
| CN103014085A (en) * | 2012-12-20 | 2013-04-03 | 通辽梅花生物科技有限公司 | Preparation method of corn syrup hydrolyzate |
| CN103642857A (en) * | 2013-09-02 | 2014-03-19 | 福建贝辰生物科技有限公司 | Production method for microbial lipid fermentation by utilization of corn steep liquor enzymatic hydrolysate |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108949864A (en) * | 2018-08-07 | 2018-12-07 | 梁山菱花生物科技有限公司 | The preparation method and application of glutamic acid fermentation sugar |
| CN111606756A (en) * | 2020-06-29 | 2020-09-01 | 黄河三角洲京博化工研究院有限公司 | Method for producing liquid fertilizer by using corn steep liquor |
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