CN107267501A - Segmented absorption magnetic bead method, storage device and nucleic acid extracting instrument - Google Patents
Segmented absorption magnetic bead method, storage device and nucleic acid extracting instrument Download PDFInfo
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- CN107267501A CN107267501A CN201710601291.3A CN201710601291A CN107267501A CN 107267501 A CN107267501 A CN 107267501A CN 201710601291 A CN201710601291 A CN 201710601291A CN 107267501 A CN107267501 A CN 107267501A
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- magnetic bead
- pause
- pause point
- nucleic acid
- magnetically attractive
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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Abstract
The present invention relates to a kind of segmented absorption magnetic bead method, storage device and nucleic acid extracting instrument, characteristic is：At least two pause points are set according to amount of reagent；The action of magnetic bead adsorbent equipment is controlled, the bar magnet on magnetic bead adsorbent equipment is enclosed on each pause point and pauses, carry out magnetic bead absorption；Pause point is set, and bar magnet is enclosed on each pause point and does appropriate pause, for segmentation magnetically attractive mode, it is ensured that fully absorbing for magnetic bead, is conducive to improving the extraction efficiency of nucleic acid.The device have the advantages that：The accommodating chamber of storage is set on support frame, it is easy to use, and succinctly.Meanwhile, support frame uses steel material, and steel have good ductility and plasticity, easily manufactured, can according to consumer demand as formed by steel bending several semiclosed accommodating chambers, to store magazine or books and other items.
Description
Technical field
It is applied to method, storage device and instrument for extracting nucleic acid that automation paramagnetic particle method extracts nucleic acid the present invention relates to a kind of
Device, more particularly to a kind of segmented absorption magnetic bead method, storage device and nucleic acid extracting instrument, introduce segmentation magnetically attractive mode,
Ensure fully absorbing for magnetic bead, improve the extraction efficiency of nucleic acid.
Background technology
When instrument for extracting nucleic acid works, the bar magnet set on magnetic bead adsorbent equipment（Bar magnet is set in bar magnet set）From test tube upper end one
Straight cutting does not do any intermediate hold to test tube bottom, to the absorption of magnetic bead not enough fully thoroughly, the extraction efficiency of nucleic acid by
To limitation.
The content of the invention
A kind of segmented absorption magnetic bead side is provided it is an object of the invention to solve the above mentioned problem of prior art presence
Method, storage device and nucleic acid extracting instrument, set pause point, and bar magnet is enclosed on each pause point and does appropriate pause, for segmentation magnetically attractive
Mode, it is ensured that fully absorbing for magnetic bead, is conducive to improving the extraction efficiency of nucleic acid.
What the above-mentioned technical purpose of the present invention was mainly solved by the following technical programs：Segmented adsorbs magnetic bead method,
Suitable for being performed instrument for extracting nucleic acid, it is characterised in that this method includes：
At least two pause points are set according to amount of reagent；
The action of magnetic bead adsorbent equipment is controlled, the bar magnet on magnetic bead adsorbent equipment is enclosed on each pause point and pauses, carry out magnetic
Pearl is adsorbed.
Pause point is set, and bar magnet is enclosed on each pause point and does appropriate pause, for segmentation magnetically attractive mode, it is ensured that magnetic bead it is abundant
Absorb, be conducive to improving the extraction efficiency of nucleic acid.
As further improving and supplementing to above-mentioned technical proposal, the present invention uses following technical measures：By amount of reagent
Total height divide equally and is segmented, and amount of reagent bottom position is a pause point, and successively up, each fragment bit is set to each
The pause point position.
Total magnetically attractive time is divided equally according to segments, the segmentation magnetically attractive time of each phase decile is formed, it is each described
The residence time of pause point is identical with the described segmentation magnetically attractive time.
Storage device, wherein a plurality of instruction that is stored with, the instruction instrument for extracting nucleic acid suitable for performing：
At least two pause points are set according to amount of reagent；
The action of magnetic bead adsorbent equipment is controlled, the bar magnet on magnetic bead adsorbent equipment is enclosed on each pause point and pauses, carry out magnetic
Pearl is adsorbed.
Amount of reagent total height divide equally and is segmented, amount of reagent bottom position is a pause point, successively up, often
Individual fragment bit is set to each pause point position.
Total magnetically attractive time is divided equally according to segments, the segmentation magnetically attractive time of each phase decile is formed, it is each described
The residence time of pause point is identical with the described segmentation magnetically attractive time.
Segmented adsorbs the instrument for extracting nucleic acid of magnetic bead, including
Processor, is adapted for carrying out each instruction；
Storage device, suitable for a plurality of instruction that is stored with, the instruction instrument for extracting nucleic acid suitable for performing：
At least two pause points are set according to amount of reagent；
The action of magnetic bead adsorbent equipment is controlled, the bar magnet on magnetic bead adsorbent equipment is enclosed on each pause point and pauses, carry out magnetic
Pearl is adsorbed.
Amount of reagent total height divide equally and is segmented, amount of reagent bottom position is a pause point, successively up, often
Individual fragment bit is set to each pause point position.
Total magnetically attractive time is divided equally according to segments, the segmentation magnetically attractive time of each phase decile is formed, it is each described
The residence time of pause point is identical with the described segmentation magnetically attractive time.
The device have the advantages that：The accommodating chamber of storage is set on support frame, it is easy to use, and succinctly.Together
When, support frame uses steel material, and steel have good ductility and plasticity, easily manufactured, can be according to consumer demand
Several semiclosed accommodating chambers as formed by steel bending, to store magazine or books and other items.
Brief description of the drawings
Fig. 1 be the present invention the instrument for extracting nucleic acid being related in magnetic rod sleeve be located at test tube upper states when structural representation
Figure.
Fig. 2 is that bar magnet is enclosed on the structural representation that uppermost pause point is paused when adsorbing magnetic bead.
Fig. 3 is that bar magnet is enclosed on the structural representation that the pause point in centre position is paused when adsorbing magnetic bead.
Fig. 4 is that bar magnet is enclosed on the structural representation that nethermost pause point is paused when adsorbing magnetic bead.
Embodiment
Below by embodiment, and with reference to accompanying drawing, technical scheme is described in further detail.
Embodiment：Segmented adsorbs magnetic bead method, it is adaptable to performed in instrument for extracting nucleic acid, including：
At least two pause points are set according to amount of reagent；Generally pause point sets 2-9, for 10 and more than,
It is feasible scheme.
The action of magnetic bead adsorbent equipment is controlled, the bar magnet on magnetic bead adsorbent equipment is enclosed on each pause point and pauses, enter
Row magnetic bead is adsorbed.
For segmentation magnetically attractive mode：Pause point is set, and bar magnet is enclosed on each pause point and does appropriate pause, it is ensured that magnetic bead it is abundant
Absorb, be conducive to improving the extraction efficiency of nucleic acid.
The experiment of segmentation magnetically attractive is carried out respectively and magnetically attractive experiment is not segmented, and same volume is added in the reagent of same volume
Magnetic bead, sets the identical magnetically attractive time.It is demonstrated experimentally that segmentation magnetically attractive is less segmented magnetically attractive, magnetically attractive effect is more thorough, and nucleic acid is carried
Take more efficient.
Amount of reagent total height divide equally and is segmented, amount of reagent bottom position is a pause point, successively up, often
Individual fragment bit is set to each pause point position.
By taking 1000ul reagent volumes as an example, it is assumed that liquid level is 9mm, three sections of magnetically attractives are set, i.e., every section liquid column length is
3mm, as described in Fig. 2-4, sets three pause points.As described in Figure 1, when preparing to proceed by adsorption experiment, magnetic bead adsorbent equipment
On bar magnet set 1（Bar magnet 3 is set in it）Positioned at the surface of test tube 2, then according to pause point 4, three absorption that pauses is carried out,
As described in Fig. 2-4, it is shown that whole magnetically attractive process.
Total magnetically attractive time is divided equally according to segments, the segmentation magnetically attractive time of each phase decile is formed, it is each described
The residence time of pause point is identical with the described segmentation magnetically attractive time.
Storage device, wherein a plurality of instruction that is stored with, the instruction instrument for extracting nucleic acid suitable for performing：
At least two pause points are set according to amount of reagent；
The action of magnetic bead adsorbent equipment is controlled, the bar magnet on magnetic bead adsorbent equipment is enclosed on each pause point and pauses, carry out magnetic
Pearl is adsorbed.
Amount of reagent total height divide equally and is segmented, amount of reagent bottom position is a pause point, successively up, often
Individual fragment bit is set to each pause point position.
Total magnetically attractive time is divided equally according to segments, the segmentation magnetically attractive time of each phase decile is formed, it is each described
The residence time of pause point is identical with the described segmentation magnetically attractive time.
Segmented adsorbs the instrument for extracting nucleic acid of magnetic bead, including
Processor, is adapted for carrying out each instruction；
Storage device, suitable for a plurality of instruction that is stored with, the instruction instrument for extracting nucleic acid suitable for performing：
At least two pause points are set according to amount of reagent；
The action of magnetic bead adsorbent equipment is controlled, the bar magnet on magnetic bead adsorbent equipment is enclosed on each pause point and pauses, carry out magnetic
Pearl is adsorbed.
Amount of reagent total height divide equally and is segmented, amount of reagent bottom position is a pause point, successively up, often
Individual fragment bit is set to each pause point position.
Total magnetically attractive time is divided equally according to segments, the segmentation magnetically attractive time of each phase decile is formed, it is each described
The residence time of pause point is identical with the described segmentation magnetically attractive time.
The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention.In the above-described embodiments,
The present invention can have various modifications and variations.Within the spirit and principles of the invention, any modification for being made, equally replace
Change, improve, should be included in the scope of the protection.
Claims (9)
1. the method that segmented adsorbs magnetic bead, it is adaptable to performed in instrument for extracting nucleic acid, it is characterised in that this method includes：
At least two pause points are set according to amount of reagent；
The action of magnetic bead adsorbent equipment is controlled, the bar magnet on magnetic bead adsorbent equipment is enclosed on each pause point and pauses, carry out magnetic
Pearl is adsorbed.
2. the method that segmented according to claim 1 adsorbs magnetic bead, it is characterised in that carry out amount of reagent total height equal
Segmentation, amount of reagent bottom position is a pause point, successively up, and each fragment bit is set to each pause point position
Put.
3. the method that segmented according to claim 1 or 2 adsorbs magnetic bead, it is characterised in that by total magnetically attractive time according to dividing
Hop count is divided equally, and forms the segmentation magnetically attractive time of each phase decile, the residence time of each pause point and described point
The section magnetically attractive time is identical.
4. storage device, it is characterised in that：Wherein be stored with a plurality of instruction, and the instruction instrument for extracting nucleic acid suitable for holding
OK：
At least two pause points are set according to amount of reagent；
The action of magnetic bead adsorbent equipment is controlled, the bar magnet on magnetic bead adsorbent equipment is enclosed on each pause point and pauses, carry out magnetic
Pearl is adsorbed.
5. storage device according to claim 4, it is characterised in that carry out amount of reagent total height to divide equally segmentation, amount of reagent
Bottom position is a pause point, and successively up, each fragment bit is set to each pause point position.
6. the storage device according to claim 4 or 5, it is characterised in that carry out total magnetically attractive time according to segments equal
Point, the segmentation magnetically attractive time of each phase decile is formed, residence time and the described segmentation magnetically attractive time of each pause point
It is identical.
7. segmented adsorbs the instrument for extracting nucleic acid of magnetic bead, it is characterised in that：Including
Processor, is adapted for carrying out each instruction；
Storage device, suitable for a plurality of instruction that is stored with, the instruction instrument for extracting nucleic acid suitable for performing：
At least two pause points are set according to amount of reagent；
The action of magnetic bead adsorbent equipment is controlled, the bar magnet on magnetic bead adsorbent equipment is enclosed on each pause point and pauses, carry out magnetic
Pearl is adsorbed.
8. segmented according to claim 7 adsorbs the instrument for extracting nucleic acid of magnetic bead, it is characterised in that by amount of reagent total height
Divide equally and be segmented, amount of reagent bottom position is pause point, successively up, each fragment bit is set to each described stop
Pause point position.
9. the segmented according to claim 7 or 8 adsorbs the instrument for extracting nucleic acid of magnetic bead, it is characterised in that by total magnetically attractive time
Divided equally according to segments, form the segmentation magnetically attractive time of each phase decile, the residence time of each pause point and institute
The segmentation magnetically attractive time stated is identical.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710601291.3A CN107267501A (en) | 2017-07-21 | 2017-07-21 | Segmented absorption magnetic bead method, storage device and nucleic acid extracting instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710601291.3A CN107267501A (en) | 2017-07-21 | 2017-07-21 | Segmented absorption magnetic bead method, storage device and nucleic acid extracting instrument |
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|---|---|
| CN107267501A true CN107267501A (en) | 2017-10-20 |
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| CN201710601291.3A Pending CN107267501A (en) | 2017-07-21 | 2017-07-21 | Segmented absorption magnetic bead method, storage device and nucleic acid extracting instrument |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN205635637U (en) * | 2016-05-24 | 2016-10-12 | 苏州英芮诚生化科技有限公司 | A mixing subassembly that is used for paramagnetic particle method nucleic acid extraction appearance and protein purification appearance |
| CN205635571U (en) * | 2016-05-24 | 2016-10-12 | 苏州英芮诚生化科技有限公司 | A mixing device that is used for paramagnetic particle method nucleic acid extraction appearance and protein purification appearance |
-
2017
- 2017-07-21 CN CN201710601291.3A patent/CN107267501A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN205635637U (en) * | 2016-05-24 | 2016-10-12 | 苏州英芮诚生化科技有限公司 | A mixing subassembly that is used for paramagnetic particle method nucleic acid extraction appearance and protein purification appearance |
| CN205635571U (en) * | 2016-05-24 | 2016-10-12 | 苏州英芮诚生化科技有限公司 | A mixing device that is used for paramagnetic particle method nucleic acid extraction appearance and protein purification appearance |
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Application publication date: 20171020 |
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