CN107245438A - A kind of device dedicated liquid bacterial classification inoculation apparatus of one-shot forming bacterium base - Google Patents

A kind of device dedicated liquid bacterial classification inoculation apparatus of one-shot forming bacterium base Download PDF

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Publication number
CN107245438A
CN107245438A CN201710308490.5A CN201710308490A CN107245438A CN 107245438 A CN107245438 A CN 107245438A CN 201710308490 A CN201710308490 A CN 201710308490A CN 107245438 A CN107245438 A CN 107245438A
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China
Prior art keywords
liquid spawn
transfer room
liquid
mixing apparatus
stirring wheel
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CN201710308490.5A
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Chinese (zh)
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CN107245438B (en
Inventor
魏宝阳
高国赋
熊慧
李朝晖
瞿兆龙
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Hunan Agricultural University
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Hunan Agricultural University
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements

Abstract

The invention belongs to Edible mushroom processing engineering device technique field, a kind of device dedicated liquid bacterial classification inoculation apparatus of one-shot forming bacterium base is disclosed, it is made up of water pump, liquid spawn pre-mixing apparatus, liquid spawn transfer room and liquid spawn storage tank;The device dedicated liquid bacterial classification inoculation apparatus of this one-shot forming bacterium base uses liquid spawn technology short time amount reproduction mycelium, so as under certain temperature and moisture condition after spray, purpose strain and lignoenzyme, cellulase collective effect, make stalk effectively and stably fast decoupled;Pass through the automation of further Improving Equipment, the pollution rate of raw material can be reduced, and reduce labor cost, compared with traditional situation, excellent mycelium can be produced, the mycelium is uniform, purity is high, can the product such as raw mushroom, beverage, fertilizer effectively facilitate development of agricultural circulation economy, pull rural area related industry to develop to high-efficiency agriculture direction;Promote recycling for forestry waste.

Description

A kind of device dedicated liquid bacterial classification inoculation apparatus of one-shot forming bacterium base
Technical field
The present invention relates to Edible mushroom processing engineering device technique field, specifically a kind of device of one-shot forming bacterium base is special Liquid bacterial vaccinating device.
Background technology
The annual about 700,000,000 tons crop stalks in China rural area, wherein there is sub-fraction to be used as deep processing raw material and livestock Feed, most of all to be burnt up as fuel, what is had directly just burns or throws away in farmland, and forestry also has substantial amounts of shrubbery and branch Fork rots naturally in sylvan life, is not utilized rationally;Many departments of country guide peasant to make full use of agriculture waste all the time Thing, improves income, but need to consume substantial amounts of manpower and materials due to handling in a traditional way it, therefore, and peasant is still so far By it when waste is treated, self-distrust is to its effective utilization.
The intrinsic biomass energy of crop stalk, at home and abroad attracts great attention already as green energy resource, but It is to be remained during the processing such as solidification, liquefaction, gasification at many technologies " bottleneck ", such as equipment investment is larger, high energy consumption and add Plus agent cost height etc.;Bacterium bag is that the culture medium plant material access strain being packed in bag is made by greenhouse culture and temperature control culture again; When producing edible mushroom, as long as keeping the moisture in specific temperature and humidity and bacterium bag, it is possible to grow edible mushroom;
The raw material of various mushrooms is different, and formula is also different;Northern August part temperature is higher, and plantation high temperature is put down Mushroom, 9, October can plant early autumn flat mushroom, bacterium bag makes, the mitogenetic material of flat mushroom, fermentation material and clinker;Material and fermentation material are that strain is put on side Side dress bag, clinker is to need to inoculate after sterilizing;General bacterium bag preparation method has following several:
1. (raw material) dispensing-spice-dress bag inoculation-culture
2. (fermentation material) dispensing-spice-, which builds heap fermentation-stirring-and builds heap fermentation-stirring-, builds heap fermentation-tune Wet-cooling-dress bag inoculation-culture
3. (clinker) dispensing-spice-dress bag-sterilizing-cooling-inoculation-culture;The device of one-shot forming bacterium base Each building block is extremely important.
The content of the invention
The invention aims to solve the problem of above is present and utilize liquid spawn technology can after simple refit Completion adds water, compressed, being inoculated with, there is provided a kind of device dedicated liquid bacterial classification inoculation apparatus of one-shot forming bacterium base for bundling and overlay film.
The present invention, which is adopted the following technical scheme that, realizes its goal of the invention:A kind of device dedicated liquid bacterium of one-shot forming bacterium base Classification inoculation apparatus is planted, it is made up of water pump 1, liquid spawn pre-mixing apparatus 2, liquid spawn transfer room 3 and liquid spawn storage tank 4;Water Pump 1 is arranged between liquid spawn pre-mixing apparatus 2 and liquid spawn storage tank 4;Liquid spawn pre-mixing apparatus 2 is arranged on liquid spawn The rear portion of transfer room 3.
The present invention is sufficiently mixed inoculation in order to realize liquid spawn and crushed material, provided with liquid spawn transfer room 3;It It is made up of liquid spawn transfer room frame 3-1, liquid spawn transfer room stirring wheel 3-2, liquid spawn transfer room stirring wheel 3-2 leads to Cross liquid spawn transfer room stirring wheel bearing seat 3-1-7 and be arranged on liquid spawn transfer room frame 3-1 inner lowers;And in liquid Strain transfer room frame 3-1 is provided with connecting tube 3-1- under connecting tube 3-1-1, liquid spawn transfer room on liquid spawn transfer room 2nd, water content shows table 3-1-3, liquid spawn pre-mixing apparatus connecting tube 3-1-6, liquid spawn transfer room stirring wheel bearing seat 3- 1-7, thermometer 3-1-5 and extension tube attached 3-1-4;Water content shows that table 3-1-3, thermometer 3-1-5 and extension tube attached 3-1-4 are installed At the top of liquid spawn transfer room frame 3-1;Connecting tube 3-1-1 is arranged on liquid spawn transfer room machine on liquid spawn transfer room Frame 3-1 upper left-hands;Connecting tube 3-1-2 is arranged on liquid spawn transfer room frame 3-1 bottoms under liquid spawn transfer room;Liquid Strain pre-mixing apparatus connecting tube 3-1-6 is arranged on liquid spawn transfer room frame 3-1 upper backs;Liquid spawn transfer room is stirred Wheel 3-2 is mixed by liquid spawn transfer room stirring wheel stirring bar 3-2-1 and liquid spawn transfer room stirring wheel central shaft 3-2-2 groups Into;Liquid spawn transfer room stirring wheel stirring bar 3-2-1 is uniformly arranged on liquid spawn transfer room stirring wheel central shaft 3-2-2 On.
The present invention is in order to realize the premix to plurality of liquid strain, provided with liquid spawn pre-mixing apparatus 2;It is by the premix that confluxes Pipe 2-1, liquid spawn pre-mixing apparatus connecting tube 2-2, shunting premix barrel 2-3 and liquid spawn pre-mixing apparatus extension tube attached 2-4 are constituted; Many shunting premix barrel 2-3 are uniformly connected to and confluxed on premix barrel 2-1;Liquid spawn pre-mixing apparatus connecting tube 2-2 is connected to remittance Flow the premix barrel 2-1 other ends;One end that liquid spawn pre-mixing apparatus extension tube attached 2-4 is located at, for UNICOM's other liquid spawns storage Tank 4.
Described liquid spawn transfer room 3 is that crushed material is transported into liquid spawn transfer room 3, and liquid spawn makees in water pump 1 Liquid spawn pre-mixing apparatus 2 is first passed through under to be premixed, and is re-introduced into liquid spawn transfer room 3 and is mixed with crushed material, in liquid Stirred in the presence of body strain transfer room stirring wheel 3-2;Water content shows that table 3-1-3 shows crushed material and liquid bacteria Mixed water content is planted, such as moisture deficit can pass through extension tube attached 3-1-4 and supplement moisture content.
As a result of above technical scheme, the present invention preferably realizes its goal of the invention, this one-shot forming bacterium base Device dedicated liquid bacterial classification inoculation apparatus uses liquid spawn technology short time amount reproduction mycelium, after spray so as to Under certain temperature and moisture condition, purpose strain and lignoenzyme, cellulase collective effect make stalk effectively and stably fast Speed is decomposed;By the automation of further Improving Equipment, the pollution rate of raw material can be reduced, and reduces labor cost, with tradition Situation compare, excellent mycelium can be produced, the mycelium is uniform, purity is high, can the product such as raw mushroom, beverage, fertilizer; Leavening can be largely produced using the production line, for handling the residues such as calabash hemp stalk, beans stalk, cornstalk, corncob and sunflower disk Thing, makes it become the good feed of palatability and the high fertilizer of nitrogen content in a short time, effectively facilitates development of agricultural circulation economy, Rural area related industry is pulled to develop to high-efficiency agriculture direction;Promote recycling for forestry waste.
Brief description of the drawings
Accompanying drawing 1 is the overall structure diagram of the present invention.
Accompanying drawing 2 is the cross-sectional view of mark 3 of the present invention.
Accompanying drawing 3 is present invention mark 3-1 structural representation.
Accompanying drawing 4 is present invention mark 3-2 structural representation.
Accompanying drawing 5 is the structural representation of mark 2 of the present invention.
Reference:Water pump 1, liquid spawn pre-mixing apparatus 2, liquid spawn transfer room 3, liquid spawn storage tank 4, liquid bacteria Plant transfer room frame 3-1, liquid spawn transfer room stirring wheel 3-2, liquid spawn transfer room stirring wheel bearing seat 3-1-7, liquid Connecting tube 3-1-2, water content show table 3-1-3, liquid bacteria under connecting tube 3-1-1, liquid spawn transfer room on strain transfer room Plant pre-mixing apparatus connecting tube 3-1-6, liquid spawn transfer room stirring wheel bearing seat 3-1-7, thermometer 3-1-5, extension tube attached 3-1- 4th, liquid spawn transfer room stirring wheel stirring bar 3-2-1, liquid spawn transfer room stirring wheel central shaft 3-2-2, premix barrel of confluxing 2-1, liquid spawn pre-mixing apparatus connecting tube 2-2, shunting premix barrel 2-3, liquid spawn pre-mixing apparatus extension tube attached 2-4.
Embodiment
The content of the invention is described further below in conjunction with the accompanying drawings:
Embodiment 1
From the content of the invention and accompanying drawing, a kind of device dedicated liquid bacterial classification inoculation apparatus of one-shot forming bacterium base, it by Water pump 1, liquid spawn pre-mixing apparatus 2, liquid spawn transfer room 3 are constituted with liquid spawn storage tank 4;Water pump 1 is arranged on liquid bacteria Plant between pre-mixing apparatus 2 and liquid spawn storage tank 4;Liquid spawn pre-mixing apparatus 2 is arranged on the rear portion of liquid spawn transfer room 3.
The present invention is sufficiently mixed inoculation in order to realize liquid spawn and crushed material, provided with liquid spawn transfer room 3;It It is made up of liquid spawn transfer room frame 3-1, liquid spawn transfer room stirring wheel 3-2, liquid spawn transfer room stirring wheel 3-2 leads to Cross liquid spawn transfer room stirring wheel bearing seat 3-1-7 and be arranged on liquid spawn transfer room frame 3-1 inner lowers;And in liquid Strain transfer room frame 3-1 is provided with connecting tube 3-1- under connecting tube 3-1-1, liquid spawn transfer room on liquid spawn transfer room 2nd, water content shows table 3-1-3, liquid spawn pre-mixing apparatus connecting tube 3-1-6, liquid spawn transfer room stirring wheel bearing seat 3- 1-7, thermometer 3-1-5 and extension tube attached 3-1-4;Water content shows that table 3-1-3, thermometer 3-1-5 and extension tube attached 3-1-4 are installed At the top of liquid spawn transfer room frame 3-1;Connecting tube 3-1-1 is arranged on liquid spawn transfer room machine on liquid spawn transfer room Frame 3-1 upper left-hands;Connecting tube 3-1-2 is arranged on liquid spawn transfer room frame 3-1 bottoms under liquid spawn transfer room;Liquid Strain pre-mixing apparatus connecting tube 3-1-6 is arranged on liquid spawn transfer room frame 3-1 upper backs;Liquid spawn transfer room is stirred Wheel 3-2 is mixed by liquid spawn transfer room stirring wheel stirring bar 3-2-1 and liquid spawn transfer room stirring wheel central shaft 3-2-2 groups Into;Liquid spawn transfer room stirring wheel stirring bar 3-2-1 is uniformly arranged on liquid spawn transfer room stirring wheel central shaft 3-2-2 On.
The present invention is in order to realize the premix to plurality of liquid strain, provided with liquid spawn pre-mixing apparatus 2;It is by the premix that confluxes Pipe 2-1, liquid spawn pre-mixing apparatus connecting tube 2-2, shunting premix barrel 2-3 and liquid spawn pre-mixing apparatus extension tube attached 2-4 are constituted; Many shunting premix barrel 2-3 are uniformly connected to and confluxed on premix barrel 2-1;Liquid spawn pre-mixing apparatus connecting tube 2-2 is connected to remittance Flow the premix barrel 2-1 other ends;One end that liquid spawn pre-mixing apparatus extension tube attached 2-4 is located at, for UNICOM's other liquid spawns storage Tank 4.
Embodiment 2
Described liquid spawn transfer room 3 is that crushed material is transported into liquid spawn transfer room 3, and liquid spawn makees in water pump 1 Liquid spawn pre-mixing apparatus 2 is first passed through under to be premixed, and is re-introduced into liquid spawn transfer room 3 and is mixed with crushed material, in liquid Stirred in the presence of body strain transfer room stirring wheel 3-2;Water content shows that table 3-1-3 shows crushed material and liquid bacteria Mixed water content is planted, such as moisture deficit can pass through extension tube attached 3-1-4 and supplement moisture content.
Although above the present invention is described in detail with a general description of the specific embodiments, On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art;Cause This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.

Claims (4)

1. the device dedicated liquid bacterial classification inoculation apparatus of a kind of one-shot forming bacterium base, it is characterized in that it is by water pump (1), liquid spawn Pre-mixing apparatus (2), liquid spawn transfer room (3) are constituted with liquid spawn storage tank (4);Water pump (1) is premixed installed in liquid spawn Between device (2) and liquid spawn storage tank (4);Liquid spawn pre-mixing apparatus (2) is arranged on liquid spawn transfer room (3) rear portion.
2. the device dedicated liquid bacterial classification inoculation apparatus of a kind of one-shot forming bacterium base as claimed in claim 1, it is characterized in that setting There is liquid spawn transfer room (3);It is by liquid spawn transfer room frame (3-1), liquid spawn transfer room stirring wheel (3-2) group Into liquid spawn transfer room stirring wheel (3-2) stirs wheel bearing seat (3-1-7) by liquid spawn transfer room and is arranged on liquid bacteria Plant transfer room frame (3-1) inner lower;And provided with connecting on liquid spawn transfer room in liquid spawn transfer room frame (3-1) Connecting tube (3-1-2), water content show that table (3-1-3), liquid spawn are loaded in mixture in advance under adapter (3-1-1), liquid spawn transfer room Put connecting tube (3-1-6), liquid spawn transfer room stirring wheel bearing seat (3-1-7), thermometer (3-1-5) and extension tube attached (3-1- 4);Water content shows that table (3-1-3), thermometer (3-1-5) are arranged on liquid spawn transfer room frame with extension tube attached (3-1-4) At the top of (3-1);Connecting tube (3-1-1) is arranged on liquid spawn transfer room frame (3-1) upper left-hand on liquid spawn transfer room; Connecting tube (3-1-2) is arranged on liquid spawn transfer room frame (3-1) bottom under liquid spawn transfer room;Liquid spawn loads in mixture in advance Connecting tube (3-1-6) is put installed in liquid spawn transfer room frame (3-1) upper back;Liquid spawn transfer room stirring wheel (3- 2) it is made up of liquid spawn transfer room stirring wheel stirring bar (3-2-1) with liquid spawn transfer room stirring wheel central shaft (3-2-2); Liquid spawn transfer room stirring wheel stirring bar (3-2-1) is uniformly arranged on liquid spawn transfer room stirring wheel central shaft (3-2-2) On.
3. a kind of device dedicated liquid bacterial classification inoculation apparatus of one-shot forming bacterium base as claimed in claim 1 or 2, it is characterized in that Provided with liquid spawn pre-mixing apparatus (2);It is by premix barrel of confluxing (2-1), liquid spawn pre-mixing apparatus connecting tube (2-2), shunting Premix barrel (2-3) is constituted with liquid spawn pre-mixing apparatus extension tube attached (2-4);Many shunting premix barrels (2-3) are uniformly connected to remittance Flow in premix barrel (2-1);Liquid spawn pre-mixing apparatus connecting tube (2-2) is connected to premix barrel of confluxing (2-1) other end;Liquid bacteria One end that pre-mixing apparatus extension tube attached (2-4) is located at is planted, for other liquid spawn storage tanks (4) of UNICOM.
4. a kind of device dedicated liquid strain inoculation dress of one-shot forming bacterium base as described in any one in claims 1 to 3 Put, it is characterized in that described liquid spawn transfer room (3) is that crushed material is transported into liquid spawn transfer room (3), liquid spawn exists Liquid spawn pre-mixing apparatus (2) is first passed through under water pump (1) effect to be premixed, and is re-introduced into liquid spawn transfer room (3) and powder Particle is mixed, and is stirred in the presence of liquid spawn transfer room stirring wheel (3-2);Water content shows table (3-1-3) Crushed material and the mixed water content of liquid spawn are shown, such as moisture deficit can supplement moisture content by extension tube attached (3-1-4).
CN201710308490.5A 2017-05-04 2017-05-04 Special liquid strain inoculation device for one-step forming of bacterial blanks Active CN107245438B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111657056A (en) * 2020-05-08 2020-09-15 谢超蔚 Be used for stuff to glue bacterial auxiliary assembly

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5248602A (en) * 1990-09-18 1993-09-28 Walter Schmid Process and installation for the utilization of organic substances
WO1998047607A1 (en) * 1997-04-24 1998-10-29 Jouvin Jean Luc Improved alginate mixer
CN101955883A (en) * 2009-07-14 2011-01-26 江南大学 Multifunctional biological solid fermentation tank
CN203916586U (en) * 2014-04-29 2014-11-05 天津市郁峰化工有限公司 A kind of dosing chamber feed system
US20150337344A1 (en) * 2012-12-27 2015-11-26 Veolia Water Solutions & Technologies Support Method for producing polyhydroxyalkanoates by microorganisms
CN205347426U (en) * 2016-01-27 2016-06-29 山西大禹生物工程有限公司 Fungus liquid automatic -mixing system

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5248602A (en) * 1990-09-18 1993-09-28 Walter Schmid Process and installation for the utilization of organic substances
WO1998047607A1 (en) * 1997-04-24 1998-10-29 Jouvin Jean Luc Improved alginate mixer
CN101955883A (en) * 2009-07-14 2011-01-26 江南大学 Multifunctional biological solid fermentation tank
US20150337344A1 (en) * 2012-12-27 2015-11-26 Veolia Water Solutions & Technologies Support Method for producing polyhydroxyalkanoates by microorganisms
CN203916586U (en) * 2014-04-29 2014-11-05 天津市郁峰化工有限公司 A kind of dosing chamber feed system
CN205347426U (en) * 2016-01-27 2016-06-29 山西大禹生物工程有限公司 Fungus liquid automatic -mixing system

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111657056A (en) * 2020-05-08 2020-09-15 谢超蔚 Be used for stuff to glue bacterial auxiliary assembly

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