CN107232210B - Thiadiazole compound is preparing the application on paddy bacterial rice shoot damping-off control medicament - Google Patents
Thiadiazole compound is preparing the application on paddy bacterial rice shoot damping-off control medicament Download PDFInfo
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- CN107232210B CN107232210B CN201710317272.8A CN201710317272A CN107232210B CN 107232210 B CN107232210 B CN 107232210B CN 201710317272 A CN201710317272 A CN 201710317272A CN 107232210 B CN107232210 B CN 107232210B
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- thiadiazoles
- sulfydryl
- rice
- plantarii
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- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 140
- 235000009566 rice Nutrition 0.000 title claims abstract description 140
- -1 Thiadiazole compound Chemical class 0.000 title claims abstract description 48
- 241000918585 Pythium aphanidermatum Species 0.000 title claims abstract description 30
- 230000001580 bacterial effect Effects 0.000 title claims abstract description 18
- 239000003814 drug Substances 0.000 title claims abstract description 12
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 139
- 241000134107 Burkholderia plantarii Species 0.000 claims abstract description 86
- 239000000575 pesticide Substances 0.000 claims abstract description 21
- GDGIVSREGUOIJZ-UHFFFAOYSA-N 5-amino-3h-1,3,4-thiadiazole-2-thione Chemical class NC1=NN=C(S)S1 GDGIVSREGUOIJZ-UHFFFAOYSA-N 0.000 claims description 52
- BIGYLAKFCGVRAN-UHFFFAOYSA-N 1,3,4-thiadiazolidine-2,5-dithione Chemical class S=C1NNC(=S)S1 BIGYLAKFCGVRAN-UHFFFAOYSA-N 0.000 claims description 40
- FPVUWZFFEGYCGB-UHFFFAOYSA-N 5-methyl-3h-1,3,4-thiadiazole-2-thione Chemical class CC1=NN=C(S)S1 FPVUWZFFEGYCGB-UHFFFAOYSA-N 0.000 claims description 26
- 239000002689 soil Substances 0.000 claims description 19
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 16
- 239000003337 fertilizer Substances 0.000 claims description 14
- YTPLMLYBLZKORZ-UHFFFAOYSA-N Divinylene sulfide Natural products C=1C=CSC=1 YTPLMLYBLZKORZ-UHFFFAOYSA-N 0.000 claims description 4
- 229930192474 thiophene Natural products 0.000 claims description 4
- 150000003851 azoles Chemical class 0.000 claims description 3
- 230000034303 cell budding Effects 0.000 claims description 2
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 2
- 239000012498 ultrapure water Substances 0.000 claims description 2
- 239000003053 toxin Substances 0.000 abstract description 23
- 231100000765 toxin Toxicity 0.000 abstract description 23
- MDYOLVRUBBJPFM-UHFFFAOYSA-N tropolone Chemical compound OC1=CC=CC=CC1=O MDYOLVRUBBJPFM-UHFFFAOYSA-N 0.000 abstract description 18
- 238000002360 preparation method Methods 0.000 abstract description 3
- 230000002265 prevention Effects 0.000 abstract description 3
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- 108700012359 toxins Proteins 0.000 description 22
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- 238000002156 mixing Methods 0.000 description 16
- 238000000034 method Methods 0.000 description 14
- GOJUJUVQIVIZAV-UHFFFAOYSA-N 2-amino-4,6-dichloropyrimidine-5-carbaldehyde Chemical group NC1=NC(Cl)=C(C=O)C(Cl)=N1 GOJUJUVQIVIZAV-UHFFFAOYSA-N 0.000 description 13
- 201000010099 disease Diseases 0.000 description 11
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 11
- 230000012010 growth Effects 0.000 description 11
- 239000007788 liquid Substances 0.000 description 10
- 150000001875 compounds Chemical class 0.000 description 9
- 241000196324 Embryophyta Species 0.000 description 8
- 229960001760 dimethyl sulfoxide Drugs 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 8
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- 238000007619 statistical method Methods 0.000 description 6
- VLLMWSRANPNYQX-UHFFFAOYSA-N thiadiazole Chemical compound C1=CSN=N1.C1=CSN=N1 VLLMWSRANPNYQX-UHFFFAOYSA-N 0.000 description 6
- 239000005794 Hymexazol Substances 0.000 description 5
- 239000000654 additive Substances 0.000 description 5
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- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 3
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- 239000007787 solid Substances 0.000 description 3
- 239000007921 spray Substances 0.000 description 3
- 150000003462 sulfoxides Chemical class 0.000 description 3
- 208000035143 Bacterial infection Diseases 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
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- 239000008103 glucose Substances 0.000 description 2
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- 230000005764 inhibitory process Effects 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- FTQWRYSLUYAIRQ-UHFFFAOYSA-N n-[(octadecanoylamino)methyl]octadecanamide Chemical class CCCCCCCCCCCCCCCCCC(=O)NCNC(=O)CCCCCCCCCCCCCCCCC FTQWRYSLUYAIRQ-UHFFFAOYSA-N 0.000 description 2
- 238000011017 operating method Methods 0.000 description 2
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- 125000003903 2-propenyl group Chemical group [H]C([*])([H])C([H])=C([H])[H] 0.000 description 1
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical class N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 1
- 241001453380 Burkholderia Species 0.000 description 1
- 229920002261 Corn starch Polymers 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 241000223218 Fusarium Species 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 229920002148 Gellan gum Polymers 0.000 description 1
- AEMRFAOFKBGASW-UHFFFAOYSA-N Glycolic acid Chemical compound OCC(O)=O AEMRFAOFKBGASW-UHFFFAOYSA-N 0.000 description 1
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- 108010076876 Keratins Proteins 0.000 description 1
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- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- WTKZEGDFNFYCGP-UHFFFAOYSA-N Pyrazole Chemical compound C=1C=NNC=1 WTKZEGDFNFYCGP-UHFFFAOYSA-N 0.000 description 1
- 241001361634 Rhizoctonia Species 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- FZWLAAWBMGSTSO-UHFFFAOYSA-N Thiazole Chemical compound C1=CSC=N1 FZWLAAWBMGSTSO-UHFFFAOYSA-N 0.000 description 1
- 235000010489 acacia gum Nutrition 0.000 description 1
- 239000001785 acacia senegal l. willd gum Substances 0.000 description 1
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- 230000016571 aggressive behavior Effects 0.000 description 1
- 150000001336 alkenes Chemical class 0.000 description 1
- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
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- 239000006227 byproduct Substances 0.000 description 1
- 239000013522 chelant Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
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- 239000003086 colorant Substances 0.000 description 1
- 238000010959 commercial synthesis reaction Methods 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 239000008120 corn starch Substances 0.000 description 1
- 229940099112 cornstarch Drugs 0.000 description 1
- 238000011161 development Methods 0.000 description 1
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- 208000034653 disorder of pilosebaceous unit Diseases 0.000 description 1
- 229910052564 epsomite Inorganic materials 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
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- 229940084434 fungoid Drugs 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000000216 gellan gum Substances 0.000 description 1
- 235000010492 gellan gum Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 208000037824 growth disorder Diseases 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 208000006278 hypochromic anemia Diseases 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- ZLTPDFXIESTBQG-UHFFFAOYSA-N isothiazole Chemical compound C=1C=NSC=1 ZLTPDFXIESTBQG-UHFFFAOYSA-N 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- HCWCAKKEBCNQJP-UHFFFAOYSA-N magnesium orthosilicate Chemical compound [Mg+2].[Mg+2].[O-][Si]([O-])([O-])[O-] HCWCAKKEBCNQJP-UHFFFAOYSA-N 0.000 description 1
- 239000000391 magnesium silicate Substances 0.000 description 1
- 229910052919 magnesium silicate Inorganic materials 0.000 description 1
- 235000019792 magnesium silicate Nutrition 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- SOQBVABWOPYFQZ-UHFFFAOYSA-N oxygen(2-);titanium(4+) Chemical compound [O-2].[O-2].[Ti+4] SOQBVABWOPYFQZ-UHFFFAOYSA-N 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000008506 pathogenesis Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000000361 pesticidal effect Effects 0.000 description 1
- 229920001592 potato starch Polymers 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 230000021749 root development Effects 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
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- 230000002195 synergetic effect Effects 0.000 description 1
- 239000000454 talc Substances 0.000 description 1
- 235000012222 talc Nutrition 0.000 description 1
- 229910052623 talc Inorganic materials 0.000 description 1
- 150000003557 thiazoles Chemical class 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/82—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with three ring hetero atoms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Toxicology (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Soil Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses thiadiazole compounds to prepare the application on paddy bacterial rice shoot damping-off control medicament, the preparation that the present invention is prepared using thiadiazole compound as principle active component, it can effectively inhibit B.plantarii to generate the level of toxin tropolone, and then prevent and treat paddy bacterial rice shoot damping-off.Simple and easy using pesticide control of the present invention prevention and treatment paddy bacterial rice shoot damping-off, at low cost, control efficiency is good.
Description
Technical field
The present invention relates to pesticide science field more particularly to thiadiazole compound prepare paddy bacterial rice shoot stand it is withered
Application on sick pesticide control.
Background technique
Plant burkholderia (Burkholderia plantarii) causes the bacillary damping-off of rice seedling
One of important pathogen, infectivity, reproductive capacity and adaptability are very strong, seriously threaten Rice Production.In nature,
B.plantarii is often distributed in seedbed soil, water in field, vegetable seeds and plant and animal residues, into rice seedling bed after in suitable condition
Lower completion First aggression, and Infection cycie again is entered by seed and soil-borne.The rice shoot infected shows as stunting in early period,
Chlorosis and root growth and development obstacle, late blight are dead.
Pathological research discovery, B.plantarii secrete a kind of small point for being referred to as tropolone (tropolone)
Sub- virulence factor forms insoluble chelate in conjunction with iron ion, induces plant shoots root system too little iron, cause growth of seedling
Slow and final withered death (such as Fig. 1).The disease was once popular and multiple in southern US and south east asia in the Japanese whole nation
Outburst, brings catastrophic loss to Rice Production.Last decade has diffused to southern US, Southeast Asia, South Korea and China.
The disease was found to report, be sent out in the multiple rice main producing regions of China at present in 2014 in China for the first time
It is raw, and with diffusion tendency.Since the seedling season of rice in China main producing region is mostly the weather conditions of high temperature and humidity, this is
Growth, breeding and the diffusion of B.plantarii creates advantageous condition.Especially the aggravation of Global Greenhouse Effect in recent years and
The popularization of greenhouse seedling-cultivation technique also aggravates the disease in the eruption and prevalence in China.
Currently, the pesticide for preventing and treating bacterial disease in the market mainly has Inorganic Copper class, organic copper class and thiazoles, such as:
Application publication number is that the application for a patent for invention document of CN106508917A discloses a kind of slow release sterilization (worm) group
Object and its application are closed, the composition is that allyl isothiazole and hymexazol composition or allyl isothiazole, hymexazol and bisamide class are killed
Worm agent composition；According to parts by weight, the weight ratio between allyl isothiazole and active group hymexazol is 1:50 to 50:1；Allyl is different
The weight fraction ratio of the sum of thiazole and hymexazol weight and bisamide insecticides is 20:1 to 200:1.Alkene in invention combination
Third isothiazole and hymexazol mixture, expand medicament to the control range of crop disease.Such as the combination is applied in the seedling cultivation of rice phase
Object, can prevent and treat simultaneously rice seedling blight (rice fungoid damping-off caused by Rhizoctonia and Fusarium), bacterial wilt and
Rice blast.
But it there is no effectively prevent medicament specifically for the paddy bacterial disease so far.Therefore, it is necessary to thin to this
Bacterium property rice shoot damping-off makes further research, and specially effectively prevents medicament to obtain.
Summary of the invention
The present invention is intended to provide the bacillary damping-off of rice seedling caused by thiadiazole compound prevention and treatment B.plantarii
New application, and in particular to thiadiazole compound is preparing the application on paddy bacterial rice shoot damping-off control medicament.
In order to realize above-mentioned purpose of the invention, the present invention provides the following technical solutions:
The present invention provides thiadiazole compounds to prepare the application on paddy bacterial rice shoot damping-off control medicament.
Preferably, the thiadiazole compound be 2- amino -5- sulfydryl -1,3,4- thiadiazoles, dimercapto -1 2,5-,
3,4- thiadiazoles or 2- sulfydryl -5- methyl-1,3,4- thiadiazoles.
The structural formula of above compound, is shown below:
2- amino -5- sulfydryl -1,3,4- thiadiazoles
2,5- dimercapto -1,3,4- thiadiazoles
2- sulfydryl -5- methyl-1,3,4- thiadiazoles
Wherein, the thiadiazole compound involved in the present invention includes using the natural extraction containing thiadiazole compound
Object, commercial synthesis object, industrial treatment by-product extract, however, it is not limited to this.In addition to above-mentioned three kinds of thiadiazole
Its derivative also can be used in compound, which refers to the salt, ester, acyl received in thiadiazole compound pesticidal preparations
The derivative of ammonia, glycocide, however, it is not limited to this.
Further, the paddy bacterial rice shoot damping-off is caused by burkholderia B.plantarii.
The present invention provides a kind of pesticide control, effective component is thiadiazole compound.Above-mentioned pesticide control, both may be used
To be single dose, i.e., pure thiadiazole compound solution (2- amino -5- sulfydryl -1,3,4- thiadiazoles or 2,5- dimercapto -1,3,
4- thiadiazoles or 2- sulfydryl -5- methyl-1,3,4- thiadiazoles), it is also possible to compound, it may be assumed that include two of them or two or more
Combination.
Preferably, the thiadiazole compound be 2- amino -5- sulfydryl -1,3,4- thiadiazoles, dimercapto -1 2,5-,
3,4- thiadiazoles and 2- sulfydryl -5- methyl-1, at least one of 3,4- thiadiazoles.
Preferably, the concentration of the thiadiazole compound is 0.005~5mM.
Further preferably, the effective component be 2- amino -5- sulfydryl -1,3,4- thiadiazoles and 2, dimercapto -1 5-,
3,4- thiadiazoles, concentration are respectively 0.005~5mM and 0.005~5mM；Alternatively, the effective component is 2- amino -5- mercapto
Base -1,3,4- thiadiazoles and 2- sulfydryl -5- methyl-1,3,4- thiadiazoles, concentration be respectively 0.005~5mM and 0.005~
5mM。
The present invention also provides a kind of pesticide controls, are calculated in mass percent, including following components:
Thiadiazole compound 0.006~0.142%；
Dimethyl sulfoxide 0.010%；
Ultrapure water 99.848~99.9839%.
Preferably, the concentration of thiadiazole compound is 0.005~5mM in above-mentioned pesticide control；Thiadiazole chemical combination
Object is 2- amino -5- sulfydryl -1,3,4- thiadiazoles, 2,5- dimercapto -1,3,4- thiadiazoles and 2- sulfydryl -5- methyl-1,3,4-
At least one of thiadiazoles.
Further preferably, the thiadiazole compound is 2- amino -5- sulfydryl -1,3,4- thiadiazoles and 2, bis- mercapto of 5-
Base -1,3,4- thiadiazoles, concentration are respectively 0.005-5mM and 0.005-5mM；It or is 2- amino -5- sulfydryl -1,3,4- thiophene
Diazole and 2- sulfydryl -5- methyl-1,3,4- thiadiazoles, concentration is respectively 0.005-5mM and 0.005-5mM；It or is 2,5-
Dimercapto-1,3,4-thiadiazole and 2- sulfydryl -5- methyl-1,3,4- thiadiazoles, concentration is respectively 0.005-5mM and 0.005-
5mM。
In addition, thiadiazole compound can be any suitable form, such as solid, semisolid, liquid or aerosol shape
Prepared by formula, acceptable carriers include, for example, water, glucose, lactose, Arabic gum, gelatin, mannitol, starch, three
Magnesium silicate, talcum, cornstarch, keratin, colloidal silicon dioxide, potato starch, sandy soil, nano-titanium dioxide, and be suitble to
Other carriers used in the preparation for preparing solid, semisolid, liquid or aerosol form；In addition said combination can contain
Stabilizer, thickener and/or colorant and fragrance, however, it is not limited to this.
Show that thiadiazole compound of the present invention generates toxin to B.plantarii through isolated experiment
The level of tropolone, which has, significantly inhibits effect.When the concentration of thiadiazole compound be 0.005mM when, toxin relative to
Control group shows reduction trend；When concentration for the treatment of rises to 0.05mM, toxin generates horizontal reduction 30%；When concentration for the treatment of liter
When to 5mM, toxin, which generates, horizontal reduces 90%.In experiment made on the living, it has been found that thiadiazole compound can be effectively suppressed
The illness for the rice seedlings that B.plantarii infects after thiadiazole compound is handled with different insecticide-applying way, reaches ideal
Control efficiency, wherein combination application also act as synergistic effect.Meanwhile thiadiazole compound can also be to the rice seedlings that do not catch an illness
Growth-promoting effect can also be played.Therefore, thiadiazole compound can be used to prepare paddy bacterial rice shoot damping-off control
Medicament.
The present invention also provides a kind of methods for preventing and treating paddy bacterial rice shoot damping-off, rice paddy seed are soaked in described
In pesticide control；Alternatively, pesticide control drop is imposed on rice seedling bed；It spreads fertilizer over the fields alternatively, the pesticide control is mixed sandy soil in rice seedling bed；
Or the pesticide control is sprayed in budding rice seedling.
Specifically, it excutes a law for drop, prior to seeding, the pesticide control is uniformly dripped and is applied to seedbed rice seedling bed water layer, keep
Water layer 7 days or more.For mixing sandy soil method, before sowing, the pesticide control is mixed thoroughly with sandy soil, uniformly spread fertilizer over the fields rice seedling bed, keeps water
Layer 7 days or more.
Compared with prior art, the invention has the following advantages:
(1) the present invention provides thiadiazole compound prevent and treat B.plantarii caused by rice seedling it is bacillary stand it is withered
The new application of disease.
(2) pesticide control provided by the invention and the method for preventing and treating paddy bacterial rice shoot damping-off can effectively inhibit
B.plantarii generates the level of toxin tropolone, and then prevents and treats paddy bacterial rice shoot damping-off.
Detailed description of the invention
Fig. 1 is the virulence factor of B.plantarii and the pathogenesis to rice seedling.
Fig. 2 is the 2- amino -5- sulfydryl -1,3,4- thiadiazoles of various concentration in embodiment 1 to B.plantarii toxin point
The influence secreted.
Fig. 3 is various concentration 2,5- dimercapto -1,3,4- thiadiazoles in embodiment 1 to B.plantarii toxin secretion
It influences.
Fig. 4 is various concentration 2- sulfydryl -5- methyl-1 in embodiment 1, and 3,4- thiadiazoles is to B.plantarii toxin secretion
Influence.
Fig. 5 is that MS culture medium adds 2- amino -5- sulfydryl -1,3,4- thiadiazoles to bacillary damping-off in embodiment 2
Typical preventive effect diagram；
Wherein, plantation is in being free of 2- amino -5- sulfydryl -1,3 after A is inoculation B.plantarii, in 4- thiadiazoles culture medium
Rice seedling；B is water of the plantation in the -5- sulfydryl -1,3,4- thiadiazoles culture medium of amino containing 2- after being inoculated with B.plantarii
Rice seedling；C is not to be inoculated with B.plantarii and plant the rice in the -5- sulfydryl -1,3,4- thiadiazoles culture medium of amino containing 2-
Seedling；D is not to be inoculated with B.plantarii and plantation in without the rice in 2- amino -5- sulfydryl -1,3,4- thiadiazoles culture medium
Seedling.
Specific embodiment
Below in conjunction with specific embodiment, the present invention will be further described.It should be understood that following embodiment is merely to illustrate this
The range of invention and is not intended to limit the present invention.
The annotation of table involved in the following example is as follows: (embodiment 2-7 and table annotate described in thiadiazole
Polymer solution or 2- amino -5- sulfydryl -1,3,4- thiadiazoles solution (0.005mM-5mM), 2,5- dimercapto -1,3,4- thiadiazoles
Solution (0.005mM-5mM), 2- sulfydryl -5- methyl-1,3,4- thiadiazoles solution (0.005mM-5mM) are referred both to through water and 1% 2
The dissolved mixed solution of methyl sulfoxide.)
Table 1-3 is 2- amino -5- sulfydryl -1,3,4- thiadiazoles, 2,5- dimercapto -1,3,4- thiadiazoles, 2- in embodiment 2
Sulfydryl -5- methyl-1, preventive effect of the 3,4- thiadiazoles in matrix additive process
Wherein, A is the rice seedlings without any processing；Plantation is in without thiadiazole after B is inoculation B.plantarii
Close the rice seedling in object culture medium；C, plantation is in containing thiadiazole compound after D, E are inoculation B.plantarii
Rice seedling in (0.005mM, 0.05mM, 5mM) culture medium；F is the rice seedlings using only 0.05mM thiadiazole compound.
Table 4-6 is 2- amino -5- sulfydryl -1,3,4- thiadiazoles, 2,5- dimercapto -1,3,4- thiadiazoles, 2- in embodiment 3
Sulfydryl -5- methyl-1, preventive effect of the 3,4- thiadiazoles in seed treatment；
Wherein, A is the rice seedlings without any processing；B is molten with 0.01% dimethyl sulfoxide after being inoculation B.plantarii
The rice seedling of liquid progress seed treatment；C, D, E use thiadiazole compound solution after being inoculation B.plantarii
The rice seedling of (0.005mM, 0.05mM, 5mM) progress seed treatment；F is the water using only 0.05mM thiadiazole compound
Rice seedling.
Table 7-9 is 2- amino -5- sulfydryl -1,3,4- thiadiazoles, 2,5- dimercapto -1,3,4- thiadiazoles, 2- in embodiment 4
Sulfydryl -5- methyl-1, preventive effect of the 3,4- thiadiazoles in spraying method；
Wherein, A is the rice seedlings without any processing；B is molten with 0.01% dimethyl sulfoxide after being inoculation B.plantarii
Liquid spray the rice seedling of processing；C, D, E use thiadiazole compound solution after being inoculation B.plantarii
(0.005mM, 0.05mM, 5mM) spray the rice seedling of processing；F is the water using only 0.05mM thiadiazole compound
Rice seedling.
Table 10-12 be embodiment 5 in 2- amino -5- sulfydryl -1,3,4- thiadiazoles, 2,5- dimercapto -1,3,4- thiadiazoles,
2- sulfydryl -5- methyl-1, preventive effect of the 3,4- thiadiazoles in excuting a law to water droplet
Wherein, A is the rice seedlings without any processing；Plantation is in 0.01% dimethyl after B is inoculation B.plantarii
Sulfoxide solution carries out the rice seedlings that processing rice seedling bed is applied to water droplet；C, with plantation in thiadiazole after D, E are inoculation B.plantarii
Compound solution (0.005mM, 0.05mM, 5mM) applies the rice seedlings in processing rice seedling bed to water droplet；F is plantation in using only 0.05mM
Thiadiazole compound applies the rice seedlings in processing rice seedling bed to water droplet.
Table 13-15 be embodiment 6 in 2- amino -5- sulfydryl -1,3,4- thiadiazoles, 2,5- dimercapto -1,3,4- thiadiazoles,
2- sulfydryl -5- methyl-1,3,4- thiadiazoles mix the preventive effect in husky broadcast application
Wherein, A is the rice seedlings without any processing；Plantation is in 0.01% dimethyl after B is inoculation B.plantarii
Sulfoxide solution mixes the rice seedlings that sandy soil spread fertilizer over the fields processing rice seedling bed；C, plantation is in thiadiazole after D, E are inoculation B.plantarii
Polymer solution (0.005mM, 0.05mM, 5mM) carries out mixing the rice seedlings that sandy soil spread fertilizer over the fields processing rice seedling bed；F is plantation in being used only
0.05mM thiadiazole compound mixes the rice seedlings that sandy soil spread fertilizer over the fields processing rice seedling bed.
Table 16-18 be embodiment 7 in 2- amino -5- sulfydryl -1,3,4- thiadiazoles, 2,5- dimercapto -1,3,4- thiadiazoles,
2- sulfydryl -5- methyl-1,3,4- thiadiazoles compounded combination liquid are mixing the preventive effect in husky broadcast application
Wherein, A is the rice seedlings without any processing；Plantation is in 0.01% dimethyl after B is inoculation B.plantarii
Sulfoxide solution mixes the rice seedlings that sandy soil spread fertilizer over the fields processing rice seedling bed；C, D, E are inoculation B.plantarii and plantation is in combination solution
(0.005mM, 0.05mM, 5mM) processes the rice seedlings in rice seedling bed；F be plantation in be used only combination solution (0.05mM) mix sandy soil
Spread fertilizer over the fields the rice seedlings in processing rice seedling bed.
Inhibiting effect of 1 thiadiazole compound of embodiment to B.plantarii toxin secretion
The culture of 1 B.plantarii
Plant burkholderia B.plantarii strain isolation is from Zhuji, zhejiang rice field, and daily culture is in 25 DEG C of constant temperature
In incubator, the culture medium of selection is nutrient broth agar culture medium.
The inoculation of 2 B.plantarii
With the single colonie of the vertical picking B.plantarii of transfer needle, places it in 50ml nutrient broth medium and carry out
Preculture after culture 24 hours, draws 10ml bacterium solution, discards supernatant liquid after 5000rpm centrifugation 5min, it is clear to add equivalent clear water
It washes, shakes after mixing, then be centrifuged 5min with 5000rpm, equivalent clear water is added again and shakes mixing, the bacterium after drawing mixing
100 μ L of liquid, which is added into 60ml MA fluid nutrient medium, to be cultivated, and the formula rate of MA culture medium is inorganic salt solution (NH4H2PO4
0.0.01%, KCl_0.02%, MgSO4·7H2O 0.02%) 200X, glucose 10g, water 1L.
Influence of the 3 2- amino -5- sulfydryl -1,3,4- thiadiazoles to the B.plantarii toxin tropolone secreted
2- amino -5- sulfydryl -1,3,4- thiadiazoles is added in the MA culture medium of inoculation B.plantarii respectively
0.005mM,0.05mM,0.5mM,5mM；And the control that leaves some space, each experiment group are repeated 3 times, will contain after the completion
The culture medium of B.plantarii and 2- amino -5- sulfydryl -1,3,4- thiadiazoles is placed on 25 DEG C of isothermal vibration cultures.Cultivation stage,
10ml bacterium solution is drawn into 50ml centrifuge tube every 12h, and after 5000rpm is centrifuged 5min, supernatant is transferred to new centrifuge tube
In, its pH is adjusted to 3,10ml acetonitrile is then added, adds 1gNaCl, after mixing fullys shake, solution is divided into two layers, inhales
Supernatant 2ml is taken, after crossing 0.22 μm of organic film, then is settled in sample injection bottle, and laggard GC-MS/MS analysis detection.
As shown in Fig. 2, working as 2- amino -5- sulfydryl -1,3, the concentration of 4- thiadiazoles is 0.005mM-5mM, B.plantarii
The level of toxin tropolone is generated, in gradient depression effect.When the concentration of 2- amino -5- sulfydryl -1,3,4- thiadiazoles is
When 0.05mM, toxin generates horizontal reduction 30%；When 2- amino -5- sulfydryl -1,3, when the concentration of 4- thiadiazoles is 5mM, toxin
The inhibiting rate of generation is up to 88%.(Fig. 2).This shows that 2- amino -5- sulfydryl -1,3,4- thiadiazoles can be effectively suppressed
The generation of B.plantarii toxin.
Influence of the 4 2,5- dimercapto -1,3,4- thiadiazoles to the B.plantarii toxin tropolone secreted
2,5- dimercapto-1,3,4-thiadiazole is added in the MA culture medium of inoculation B.plantarii respectively, 0.005mM,
0.05mM, 0.5mM, 5mM, and the control that leaves some space, each experiment group are repeated 3 times, after the completion will containing B.plantarii and
The culture medium of 2,5- dimercapto -1,3,4- thiadiazoles is placed on 25 DEG C of isothermal vibration cultures.In culture to 96h, 10ml bacterium solution is drawn,
GC-MS/MS analysis detection toxin situation.
As shown in figure 3, when the concentration of 2,5- dimercapto-1,3,4-thiadiazole is 0.005mM-5mM, B.plantarii
Toxin generation is suppressed, and certain dose-dependent effect is presented, and maximal percentage inhibition is up to 86%.This shows 2,5-, bis- mercapto
The generation of B.plantarii toxin can be effectively suppressed in base -1,3,4- thiadiazoles.
5 2- sulfydryl -5- methyl-1s, influence of the 3,4- thiadiazoles to the B.plantarii toxin tropolone secreted
The addition 2- sulfydryl -5- methyl-1 in the MA culture medium of inoculation B.plantarii respectively, 3,4- thiadiazoles,
0.005mM, 0.05mM, 0.5mM, 5mM, and the control that leaves some space, each experiment group are repeated 3 times, will contain after the completion
B.plantarii and 2- sulfydryl -5- methyl-1, the culture medium of 3,4- thiadiazoles are placed on 25 DEG C of isothermal vibration cultures.It is cultivating extremely
96h draws 10ml bacterium solution, GC-MS/MS analysis detection toxin situation.
As shown in figure 4, work as 2- sulfydryl -5- methyl-1, when the concentration of 3,4- thiadiazoles is 0.005mM-5mM,
The generation of B.plantarii toxin is suppressed, and certain dose-dependent effect is presented, and maximal percentage inhibition is up to 79%.This table
Bright, the generation of B.plantarii toxin can be effectively suppressed in 2- sulfydryl -5- methyl-1,3,4- thiadiazoles.
2 growth substrate additive process of embodiment
1 rice vernalization
Sufficient amount of rice paddy seed is chosen, after carrying out surface sterilization half an hour using 2% sodium hypochlorite, after sufficiently cleaning
It places it in sterilizing glass culture dish, adds a small amount of sterilizing clear water (be subject to and do not had the surface of the seed), be placed on 25 later
It is cultivated in DEG C constant incubator, every other day needs replacing primary sterilizing clear water, after 2-3 days, the phenomenon that shows money or valuables one carries unintentionally occurs in seed, at this time
Vernalization is completed.
2 inoculation B.plantarii
With the single colonie of the vertical picking B.plantarii of transfer needle, places it in 50ml nutrient broth medium and carry out
Preculture after culture 24 hours, draws 10ml bacterium solution, discards supernatant liquid after 5000rpm centrifugation 5min, it is clear to add equivalent clear water
It washes, shakes after mixing, then be centrifuged 5min with 5000rpm, equivalent clear water is added again and shakes mixing.After the completion of vernalization
Rice paddy seed is divided into two batches, is separately added into and prepares B.plantarii bacteria suspension as inoculation group or isometric sterilizing clear water
It (is subject to as not inoculation group and did not had the surface of the seed), cultivate 24 hours.
Efficiency test of the 3 2- amino -5- sulfydryl -1,3,4- thiadiazoles to infection B.plantarii rice seedlings
The MS plant culture of certain volume is prepared, adds 2- amino -5- sulfydryl -1,3,4- thiadiazoles respectively
(0.005mM, 0.05mM, 5mM) and 2- amino -5- sulfydryl -1,3 is not added, 4- thiadiazoles is dispensed into culture bottle, every bottle
The gellan gum of 20ml, every bottle of addition 1.5% become solid medium.Then B.plantarii will be inoculated with and be not inoculated with
The rice paddy seed of B.plantarii, plantation is trained to containing and not containing 2- amino -5- sulfydryl -1,3, the MS of 4- thiadiazoles respectively
Feeding base, 12 every bottle.After each experiment group is provided with, and culture bottle is put into 25 DEG C of constant incubators, daily illumination
12h dark 12h cultivates rice seedlings, its upgrowth situation of continuous observation, and on day 3 when measure rice seedlings root and leaf length
Degree, it is for statistical analysis.
As a result, it has been found that being compared with the rice seedlings for not being inoculated with B.plantarii, the rice seedlings table after being inoculated with B.plantarii
Reveal apparent illness (yellow and stunt), overground part and root growth are significantly inhibited (Fig. 5 A)；Inoculation
After addition 2- amino -5- sulfydryl -1,3,4- thiadiazoles, ongoing disease is relieved (Fig. 5 B) B.plantarii rice seedlings, and
The rice growth situation for not being inoculated with B.plantarii is close；Meanwhile comparison is not inoculated with the rice seedlings of B.plantarii, adds 2-
Amino -5- sulfydryl -1,3,4- thiadiazoles can also play slight facilitation (Fig. 5 C and Fig. 5 D) to paddy growth.In addition, water
The root of rice seedling and the length statistical analysis of leaf show (table 1) that 2- amino -5- sulfydryl -1,3,4- thiadiazoles can effectively control
The bacillary damping-off of rice seedling caused by B.plantarii.
Preventive effect of the 1. 2- amino -5- sulfydryl -1,3,4- thiadiazoles of table in matrix additive process
| Processing | Stem length (cm) | Root long (cm) |
| A | 4.5 | 1.8 |
| B | 0.8 | 0.2 |
| C | 3.5** | 0.8** |
| D | 4.2** | 1.5** |
| E | 4.8** | 1.9** |
| F | 5.1 | 2 |
**P<0.005
As shown in Table 1, it is compared with the paddy growth situation without any processing, is only inoculated with the rice after B.plantarii
Growth is obviously suppressed, and 2- amino -5- sulfydryl -1,3 is used only, and the paddy growth of 4- thiadiazoles processing has but obtained slightly
Promotion (P ﹤ 0.05).In addition, inoculation B.plantarii and 2- amino -5- sulfydryl -1,3 using 0.005mM-5mM, 4- thiophene
The growth inhibition of the rice seedlings of diazole processing has obtained apparent alleviation (P ﹤ 0.005).By comparing different experiments group rice
The root of seedling and the length (table 1) of leaf, we have also obtained conclusion similar to the above, this illustrates 2- amino -5- sulfydryl -1,3,4-
Thiadiazoles can effectively prevent B.plantarii and infect caused rice shoot damping-off after rice.
Efficiency test of the 4 2,5- dimercapto -1,3,4- thiadiazoles to infection B.plantarii rice seedlings
Operating procedure is according to " preventive effect of the 2- amino -5- sulfydryl -1,3,4- thiadiazoles to infection B.plantarii rice seedlings
Test " carries out, and test compound is only changed to 2,5- dimercapto-1,3,4-thiadiazole.
Preventive effect of the 2. 2,5- dimercapto -1,3,4- thiadiazoles of table in matrix additive process
| Processing | Stem length (cm) | Root long (cm) |
| A | 4.3 | 1.6 |
| B | 0.5 | 0.2 |
| C | 3.7** | 1.0** |
| D | 3.9** | 1.4** |
| E | 4.4** | 1.7** |
| F | 4.5 | 1.5 |
**P<0.005
As shown in Table 2, the rice seedlings of B.plantarii are inoculated with, 2,5- dimercapto -1,3 through 0.005mM-5mM, 4- thiophene
Diazole processing, disease symptom obtain significantly alleviating (P ﹤ 0.005).Illustrate that 2,5- dimercapto -1,3,4- thiadiazoles can effectively be prevented
It controls B.plantarii and infects caused rice shoot damping-off after rice.
5 2- sulfydryl -5- methyl-1s, efficiency test of the 3,4- thiadiazoles to infection B.plantarii rice seedlings
Operating procedure is according to " preventive effect of the 2- amino -5- sulfydryl -1,3,4- thiadiazoles to infection B.plantarii rice seedlings
Test " carries out, and test compound is only changed to 2- sulfydryl -5- methyl-1,3,4- thiadiazoles.
3. 2- sulfydryl -5- methyl-1 of table, preventive effect of the 3,4- thiadiazoles in matrix additive process
**P<0.005
As shown in Table 3, the rice seedlings of B.plantarii are inoculated with, the 2- sulfydryl -5- methyl-1 through 0.005mM-5mM, 3,4-
Thiadiazoles processing, disease symptom obtain significantly alleviating (P ﹤ 0.005).Illustrate that 2- sulfydryl -5- methyl-1,3,4- thiadiazoles can have
Effect prevention and treatment B.plantarii infects caused rice shoot damping-off after rice.
3 seed treatment of embodiment
2- amino -5- sulfydryl -1,3,4- thiadiazoles solution (0.005mM-5mM), 2,5- dimercapto -1,3,4- thiophene two respectively
Azoles solution (0.005mM-5mM), 2- sulfydryl -5- methyl-1,3,4- thiadiazoles solution (0.005mM-5mM), 0.01% dimethyl
Sulfoxide solution carries out vernalization, and the rice paddy seed after the completion of vernalization is divided into four batches.Every batch of, which is separately added into, prepares B.plantarii
Bacteria suspension, as not inoculation group (be subject to and do not had the surface of the seed), it is small to cultivate 24 as inoculation group or isometric sterilizing clear water
When.It is planted respectively in MS plant culture, 12 every bottle.And culture bottle is put into 25 DEG C of constant incubators, daily illumination 12h
Dark 12h cultivates rice seedlings, its upgrowth situation of continuous observation, and on day 3 when measure rice seedlings root and leaf length,
It is for statistical analysis.
Preventive effect of the 4. 2- amino -5- sulfydryl -1,3,4- thiadiazoles of table in seed treatment
| Processing | Stem length (cm) | Root long (cm) |
| A | 4.8 | 2.1 |
| B | 1 | 0.3 |
| C | 2.8** | 0.8** |
| D | 3.5** | 1.2** |
| E | 4.1** | 1.4** |
| F | 4.9 | 1.8 |
**P<0.005
Preventive effect of the 5. 2,5- dimercapto -1,3,4- thiadiazoles of table in seed treatment
| Processing | Stem length (cm) | Root long (cm) |
| A | 4.6 | 1.7 |
| B | 0.7 | 0.4 |
| C | 3.2** | 0.8** |
| D | 3.7** | 1.5** |
| E | 4.2** | 1.5** |
| F | 4.7 | 1.6 |
**P<0.005
6. 2- sulfydryl -5- methyl-1 of table, preventive effect of the 3,4- thiadiazoles in seed treatment
| Processing | Stem length (cm) | Root long (cm) |
| A | 4.3 | 1.8 |
| B | 0.8 | 0.3 |
| C | 2.5** | 0.5** |
| D | 2.9** | 0.7** |
| E | 3.5** | 1.2** |
| F | 4.7 | 2 |
**P<0.005
As a result as shown in Table 4-6, compared with the paddy growth situation without any processing, after being only inoculated with B.plantarii
Paddy growth be obviously suppressed, and use 2- amino -5- sulfydryl -1,3,4- thiadiazoles solution (0.005mM-5mM), 2,5-
Dimercapto -1,3,4- thiadiazoles solution (0.005mM-5mM), 2- sulfydryl -5- methyl-1,3,4- thiadiazoles solution (0.005mM-
5mM) paddy growth of seed soaking has but obtained slight promotion (P ﹤ 0.05).In addition, after being inoculated with B.plantarii, hair
Disease symptoms are significantly suppressed (P ﹤ 0.005).This explanation uses 2- amino -5- sulfydryl -1,3,4- thiadiazoles solution, 2,5- bis-
Sulfydryl -1,3,4- thiadiazoles solution, 2- sulfydryl -5- methyl-1,3,4- thiadiazoles solution carry out seed soaking, energy to rice paddy seed
It effectively prevents B.plantarii and infects caused rice shoot damping-off after rice.
4 spraying method of embodiment
Rice vernalization and inoculation B.plantarii are with embodiment 2, and plantation is in MS plant culture, and 12 every bottle.And it will training
Feeding bottle is put into 25 DEG C of constant incubators, and daily illumination 12h dark 12h cultivates rice seedlings, after 1 day, to rice seedling
Spray 2- amino -5- sulfydryl -1,3,4- thiadiazoles solution (0.005mM-5mM), 2,5- dimercapto -1,3,4- thiadiazoles solution
(0.005mM-5mM), 2- sulfydryl -5- methyl-1,3,4- thiadiazoles solution (0.005mM-5mM), 0.01% dimethyl sulfoxide are molten
Liquid, and on day 3 when measure rice seedlings root and leaf length, it is for statistical analysis.
Preventive effect of the 7. 2- amino -5- sulfydryl -1,3,4- thiadiazoles of table in spraying method method
| Processing | Stem length (cm) | Root long (cm) |
| A | 4.5 | 1.7 |
| B | 0.3 | 0.2 |
| C | 3.3** | 1** |
| D | 4.1** | 1.5** |
| E | 4.8** | 1.8** |
| F | 4.7 | 2 |
**P<0.005
Preventive effect of the 8. 2,5- dimercapto -1,3,4- thiadiazoles of table in spraying method
**P<0.005
9. 2- sulfydryl -5- methyl-1 of table, preventive effect of the 3,4- thiadiazoles in spraying method
| Processing | Stem length (cm) | Root long (cm) |
| A | 5.1 | 2.2 |
| B | 0.2 | 0.0 |
| C | 4.2** | 1.4** |
| D | 4.5** | 1.7** |
| E | 5.0** | 2.4** |
| F | 4.9 | 2.0 |
**P<0.005
As a result as shown in table 7-9,2- amino -5- sulfydryl -1,3,4- thiadiazoles solution (0.005mM-5mM), 2,5- are used
Dimercapto -1,3,4- thiadiazoles solution (0.005mM-5mM), 2- sulfydryl -5- methyl-1,3,4- thiadiazoles solution (0.005mM-
After 5mM) spraying, the disease symptom of rice seedling is significantly suppressed (P ﹤ 0.005).This explanation uses 2- amino -5- sulfydryl -
1,3,4- thiadiazoles solution, 2,5- dimercapto -1,3,4- thiadiazoles solution, 2- sulfydryl -5- methyl-1,3,4- thiadiazoles solution pair
Rice seedling carries out spraying processing, can effectively prevent B.plantarii and infect caused rice shoot damping-off after rice.
Embodiment 5 excutes a law to water droplet
By 2- amino -5- sulfydryl -1,3,4- thiadiazoles solution (0.005mM-5mM), 2,5- dimercapto -1,3,4- thiadiazoles
Solution (0.005mM-5mM), 2- sulfydryl -5- methyl-1,3,4- thiadiazoles solution (0.005mM-5mM) are administered according to drop per acre
5 liters of drops of liquid are applied, and control group only drips the aqueous solution for applying 0.01% isometric dimethyl sulfoxide, are kept for water layer one week.Rice is urged
Bud and inoculation B.plantarii are the same as embodiment 2.It sows in above-mentioned application and the rice seedling bed not being administered, and was measured at the 20th day
The length of rice seedlings root and leaf, it is for statistical analysis.
Preventive effect of the 10. 2- amino -5- sulfydryl -1,3,4- thiadiazoles of table in excuting a law to water droplet
| Processing | Stem length (cm) | Root long (cm) |
| A | 18.4 | 8.7 |
| B | 5.2 | 2.2 |
| C | 12.5** | 4.5** |
| D | 15.1** | 7.1** |
| E | 17.8** | 9** |
| F | 19.2 | 9.5 |
**P<0.005
Preventive effect of the 11. 2,5- dimercapto -1,3,4- thiadiazoles of table in excuting a law to water droplet
| Processing | Stem length (cm) | Root long (cm) |
| A | 18.1 | 8.5 |
| B | 4.0 | 1.5 |
| C | 13.8** | 5** |
| D | 16.5** | 6.3** |
| E | 18.4** | 8.8** |
| F | 18.3 | 8.6 |
**P<0.005
12. 2- sulfydryl -5- methyl-1 of table, preventive effect of the 3,4- thiadiazoles in excuting a law to water droplet
| Processing | Stem length (cm) | Root long (cm) |
| A | 18.9 | 9.2 |
| B | 4.3 | 1.6 |
| C | 10.4** | 3.7** |
| D | 12.5** | 4.4** |
| E | 15.0** | 5.1** |
| F | 18.2 | 8.4 |
**P<0.005
As a result as shown in table 10-12, using 2- amino -5- sulfydryl -1,3,4- thiadiazoles solution (0.005mM-5mM), 2,
5- dimercapto -1,3,4- thiadiazoles solution (0.005mM-5mM), 2- sulfydryl -5- methyl-1,3,4- thiadiazoles solution
After (0.005mM-5mM) applies water droplet, the disease symptom of rice seedling is significantly suppressed (P ﹤ 0.005).This illustrates to use
2- amino -5- sulfydryl -1,3,4- thiadiazoles solution, 2,5- dimercapto -1,3,4- thiadiazoles solution, 2- sulfydryl -5- methyl-1,3,
After 4- thiadiazoles solution applies processing rice seedling bed to water droplet, it can effectively prevent rice shoot damping-off caused by B.plantarii.
Embodiment 6 mixes sandy soil broadcast application
According to 3 kilograms of sandy soil are spread fertilizer over the fields per acre, respectively with 2- amino -5- sulfydryl -1,3,4- thiadiazoles solution (0.005mM-
5mM), 2,5- dimercapto -1,3,4- thiadiazoles solution (0.005mM-5mM), 2- sulfydryl -5- methyl-1,3,4- thiadiazoles solution
(0.005mM-5mM) is mixed thoroughly, and control group is mixed thoroughly using 0.01% isometric dimethyl sulphoxide aqueous solution with sandy soil.After spreading fertilizer over the fields,
Kept for water layer one week.Rice vernalization and inoculation B.plantarii are the same as embodiment 2.In above-mentioned application and the rice seedling bed not being administered
It sows, and measured the length of rice seedlings root and leaf at the 20th day, it is for statistical analysis.
13. 2- amino -5- sulfydryl -1,3,4- thiadiazoles of table is mixing the preventive effect in native broadcast application
**P<0.005
14. 2,5- dimercapto -1,3,4- thiadiazoles of table is mixing the preventive effect in native broadcast application
| Processing | Stem length (cm) | Root long (cm) |
| A | 18.8 | 8.2 |
| B | 2.6 | 1.5 |
| C | 11.0** | 4.8** |
| D | 13.2** | 5.9** |
| E | 16.9** | 8.3** |
| F | 18.2 | 8.4 |
**P<0.005
15. 2- sulfydryl -5- methyl-1 of table, 3,4- thiadiazoles are mixing the preventive effect in native broadcast application
| Processing | Stem length (cm) | Root long (cm) |
| A | 20.2 | 9.3 |
| B | 4.4 | 2.8 |
| C | 10.5** | 5.6** |
| D | 15.5** | 6.5** |
| E | 16.4** | 8.2** |
| F | 18.9 | 8.7 |
**P<0.005
As a result as shown in table 13-15, using 2- amino -5- sulfydryl -1,3,4- thiadiazoles solution (0.005mM-5mM), 2,
5- dimercapto -1,3,4- thiadiazoles solution (0.005mM-5mM), 2- sulfydryl -5- methyl-1,3,4- thiadiazoles solution
(0.005mM-5mM) is mixed after sandy soil spread fertilizer over the fields, and the disease symptom of rice seedling is significantly suppressed (P ﹤ 0.005).This explanation is adopted
With 2- amino -5- sulfydryl -1,3,4- thiadiazoles solution, 2,5- dimercapto -1,3,4- thiadiazoles solution, 2- sulfydryl -5- methyl-1,
3,4- thiadiazoles solution mix soil spread fertilizer over the fields processing rice seedling bed after, can effectively prevent rice shoot damping-off caused by B.plantarii.
Embodiment 7 mixes the medical fluid combined treatment in sandy soil broadcast application
Implementing procedure is the same as embodiment 6.Experiment medical fluid is only changed to combination solution I:2- amino -5- sulfydryl -1,3,4- thiophene two
Azoles and 2, (the two compound proportion is 1:1, concentration range 0.005mM- to the combination solution of 5- dimercapto-1,3,4-thiadiazole
In the combination solution of 5mM, 0.005mM be 2- amino -5- sulfydryl -1,3 containing 0.005mM, 4- thiadiazoles and 0.005mM 2,
5- dimercapto-1,3,4-thiadiazole, similarly hereinafter)；Combination solution II:2- amino -5- sulfydryl -1,3,4- thiadiazoles and 2- sulfydryl -5-
Methyl-1, the combination solution of 3,4- thiadiazoles (the two compound proportion is 1:1, concentration 0.005mM-5mM)；Combination solution III:
2,5- dimercapto -1,3,4- thiadiazoles and 2- sulfydryl -5- methyl-1, (the two compound proportion is the combination solution of 3,4- thiadiazoles
1:1, concentration 0.005mM-5mM).Remaining experimental setup process and condition are same as Example 6.
16. combined treatment I of table is mixing the preventive effect in native broadcast application
**P<0.005
17. combined treatment II of table is mixing the preventive effect in native broadcast application
| Processing | Stem length (cm) | Root long (cm) |
| A | 20.6 | 7.8 |
| B | 4.0 | 1.3 |
| C | 12.4** | 5.2** |
| D | 14.3** | 7.0** |
| E | 17.9** | 7.5** |
| F | 18.6 | 8.2 |
**P<0.005
18. combined treatment III of table is mixing the preventive effect in native broadcast application
| Processing | Stem length (cm) | Root long (cm) |
| A | 20.2 | 7.5 |
| B | 4.2 | 1.1 |
| C | 10.8** | 4.9** |
| D | 13.7** | 6.1** |
| E | 16.1** | 6.7** |
| F | 19.5 | 8.1 |
**P<0.005
As a result as shown in table 16-18, the morbidity of rice seedling after sandy soil spread fertilizer over the fields is mixed respectively using combination solution I, II, III
Symptom is significantly suppressed (P ﹤ 0.005).This explanation utilizes 2- amino -5- sulfydryl -1,3,4- thiadiazoles solution, bis- mercapto of 2,5-
Base -1,3,4- thiadiazoles solution, 2- sulfydryl -5- methyl-1, both any compounding in 3,4- thiadiazoles threes are carried out mixing soil and be spread fertilizer over the fields
Rice seedling bed is handled, rice shoot damping-off caused by B.plantarii can be effectively prevented.
The above enumerated are only specific embodiments of the present invention for finally, it should also be noted that.Obviously, the present invention is unlimited
In above embodiments, acceptable there are many deformations.Those skilled in the art can directly lead from present disclosure
Out or all deformations for associating, it is considered as protection scope of the present invention.
Claims (3)
1. thiadiazole compound is preparing the application on paddy bacterial rice shoot damping-off control medicament, which is characterized in that thiophene
Diazoles compound is 2- amino -5- sulfydryl -1,3,4- thiadiazoles, 2,5- dimercapto -1,3,4- thiadiazoles and 2- sulfydryl -5- first
At least one of base -1,3,4- thiadiazoles；The paddy bacterial rice shoot damping-off is by burkholderia B.plantarii
Cause.
2. a kind of thiadiazole compound according to claim 1 is preparing paddy bacterial rice shoot damping-off control medicament
On application, which is characterized in that rice paddy seed is soaked in pesticide control；Alternatively, pesticide control drop is imposed on rice seedling bed；Or
Pesticide control is mixed sandy soil and spread fertilizer over the fields in rice seedling bed by person；Or pesticide control is sprayed in budding rice seedling；
The effective component of the pesticide control is 2- amino -5- sulfydryl -1,3,4- thiadiazoles and 2,5- dimercapto -1,3,4- thiophene two
Azoles, concentration are respectively 0.005~5mM；Alternatively, the effective component is 2- amino -5- sulfydryl -1,3,4- thiadiazoles and 2- mercapto
Base -5- methyl-1,3,4- thiadiazoles, concentration are respectively 0.005~5mM；Or the effective component is dimercapto -1 2,5-,
3,4- thiadiazoles and 2- sulfydryl -5- methyl-1,3,4- thiadiazoles, concentration is respectively 0.005~5mM.
3. thiadiazole compound according to claim 2 is on preparing paddy bacterial rice shoot damping-off control medicament
Using, which is characterized in that the pesticide control is calculated in mass percent, including following components:
Thiadiazole compound 0.006~0.142%；
Dimethyl sulfoxide 0.010%；
Ultrapure water 99.848~99.9839%；
The thiadiazole compound be 2- amino -5- sulfydryl -1,3,4- thiadiazoles and 2,5- dimercapto-1,3,4-thiadiazole,
Its concentration is respectively 0.005~5mM；Alternatively, the thiadiazole compound be 2- amino -5- sulfydryl -1,3,4- thiadiazoles and
2- sulfydryl -5- methyl-1,3,4- thiadiazoles, concentration is respectively 0.005~5mM；Or the thiadiazole compound is 2,
5- dimercapto-1,3,4-thiadiazole and 2- sulfydryl -5- methyl-1,3,4- thiadiazoles, concentration is respectively 0.005~5mM.
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