CN107228944A - The new application and application method of a kind of immune detector and its reagent in operation - Google Patents
The new application and application method of a kind of immune detector and its reagent in operation Download PDFInfo
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- CN107228944A CN107228944A CN201710336319.5A CN201710336319A CN107228944A CN 107228944 A CN107228944 A CN 107228944A CN 201710336319 A CN201710336319 A CN 201710336319A CN 107228944 A CN107228944 A CN 107228944A
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- parathormone
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/04—Endocrine or metabolic disorders
- G01N2800/046—Thyroid disorders
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Abstract
The present invention relates to new application in operation of a kind of immune detector and its reagent and application method, it is characterised in that:Use it for the identification of parathyroid gland.It can complete detection in 12 minutes, and quick detection pattern can be used, multiple detection sites are detected simultaneously, and it is simple to operate, cost is relatively low, its Cleaning Principle is that parathormone detection reagent uses Colloidal Gold, solid-phase immunity chromatographic technique, pass through the principle of double antibody sandwich method, parathyroid hormone levels are quantitative determined with parathyroid gland cellulose content direct proportionality according to the depth of detection line color, sensitivity and the accuracy of detection are substantially increased as tracer material because employing bulky grain collaurum (100nm) particle.Judge whether suspect tissue is parathyroid gland according further to Concentration Testing result is objective, as a result more accurately and reliably.
Description
Technical field
The present invention relates to medical field, new application in operation of particularly a kind of immune detector and its reagent and use
Method
Background technology
Application No. 201610851934.5, Authorization Notice No. is CN106370836A, a kind of immune detector and its is made
A kind of immune detector is disclosed with method, its model one hundred in actual production is difficult to understand up to immune detector, for human body
Serum, blood plasma, the detection mark (wherein comprising parathormone) of whole blood carry out quantitative detecting analysis, and auxiliary is provided for clinic
Diagnosis, but in clinical practice, it has been found that immune detector joint parathormone detection reagent can be in thyroid operation
The middle parathyroid gland cellulose content for quantitative determining the tissue eluent extracted from tissue, its measurement result is quick, accurate.And secrete
Parathormone is that the parathyroid hormone cellulose content in the exclusive physiological function of parathyroid gland, its body of gland is far above its hetero-organization,
Therefore this immune detector and its kit can be used for the identification of parathyroid gland, so as to be created conditions for protection parathyroid gland.
Thyroid cancer is that the global range incidence of disease rises most fast solid malignant, and thyroid gland is cut off entirely and central area drenches
Fawn on to clean and turn into the conventional operative treatment mode of thyroid cancer, but postoperative parathyroid gland work(caused by parathyroid injury
Can be still lowly the problem for perplexing Thyroid Surgery doctor, parathyroid injury mainly includes contusion, blood supply obstacle and cut by mistake.First
The incidence of the postoperative temporary and permanent hypoparathyroidism of shape gland is respectively 14%-60% and 4%-11%, temporarily
Property hypoparathyroidism can cause transient low calcium symptom, but big influence is not resulted in quality of life of patients;And forever
Long property hypoparathyroidism can then cause permanent low calcium symptom, more using numb in every limb and tic of limbs as performance, sternly
Ghost image rings the quality of life of patient, and as the principal element for producing medical tangle.Therefore, first shape in thyroid operation should be paid attention to
The protection of other gland, it is to avoid operation mistiming cuts accidental injury parathyroid gland.
But parathyroid gland is incretory, majority is flat ellipse corpusculum, and live body is in brown color, just as the grain of rice or like pressure
Flat soya bean, diameter about 3~6mm, outer lamellate connective tissue membrane parcel.The number of parathyroid gland is indefinite, and document report is about
48%~62% Chinese have 4 pieces, but also may occur in which by the variation less than 4 pieces or up to more than ten, and the first shape made a variation
Its shape size of gland and color have a variation, shape can because of lesion in the form of sheets, it is wire, streak, size also can be because of lesion
Atrophy or hyperplasia, color and luster show fatty color because being coated by fat.Which increase the difficulty of identification.And recognize the tradition of parathyroid gland
Method mainly has naked eyes method of identification, hydrometer method, frozen section pathologic finding method, methylenum careuleum positive development method, nano-sized carbon negative development method,
But naked eyes method of identification is more subjective, error is larger;And hydrometer method in situ can not be recognized, isolated measuring is needed, and other gland is fatty
During parcel, it will also bubble through the water column, it is likely that be taken as fat globule and cut by mistake;Frozen section pathologic finding then complex operation, identification
Time longer (being more than 30 minutes) needs patient to wait result in operation, then does next step operation, procrastinates operating time;It is sub-
Though first indigo plant positive development method is more directly perceived, as a kind of medicine, over adaptation disease medication, side effect is big, and None- identified makes a variation
Parathyroid gland;Though nano-sized carbon negative development method accuracy rate is higher, fat and other gland are also usually cannot be distinguished by, when lymphatic vessel resistance
During plug, it is likely that mislead the judgement of patient, mistake retains the lymph node of existing cancer metastasis, and consequence is serious and costly.Cause
Quick, accurate, the economic recognition methods of this parathyroid gland has actual clinical meaning.
The content of the invention
A technical problem to be solved by this invention is to provide Application No. 201610851934.5, Authorization Notice No.
For CN106370836A, a kind of immune detector disclosed in entitled a kind of immune detector and its application method is real
The new application difficult to understand for reaching immune detector of model one hundred in the production of border.
Another technical problem to be solved by this invention is to provide the operating method of this new application and its provided this
The parathormone detection kit being related in the operating method of new application.
What the present invention reached has the technical effect that:
1. accurate, selectivity is high, the pouring around the concentration of parathyroid hormone in detection " tissue eluent ", significantly differentiation
Fawn on, fat etc..
2. anti-interference, secretion parathormone is the unique physiological function of parathyroid gland, therefore operative site tissues and device
The complicated physiological pathology background of official does not influence measurement result.
3. it is quick, it can be judged in 12 minutes, not procrastinate operating time.
4. simple to operate, judgement is directly perceived, and the people of operating procedure 1-2 can complete, and directly give the concentration of parathyroid hormone
Numerical value makes a decision.
5. economical, cost is relatively low, does not increase patient burden.
6. multiple spot is recognized, multiple positions in operation can be identified simultaneously.
The present invention solves above-mentioned technical problem and adopted the technical scheme that:Application No. 201610851934.5, authorizes public
Announcement number is CN106370836A, a kind of immune detector disclosed in entitled a kind of immune detector and its application method
New application, the identification for parathyroid gland.
Further, its purposes in the identification of parathyroid gland is the group by being extracted to doubtful Parathyroid Tissue
Knit eluent and detect and reach identifying purpose.
The present inventor has found through widely studied experiment, Application No. 201610851934.5, and Authorization Notice No. is
A kind of CN106370836A, immune detector disclosed in a kind of immune detector and its application method is used for the knowledge of parathyroid gland
There can be fast and accurately result on not.
Its specifically used method for being used for new application is:
In order to solve the above technical problems, the present invention is realized as follows:
Step 1. is started shooting, and selects detection pattern, to be checked.
Step 2. extracts the tissue eluent of doubtful parathyroid gland.
Step 3. adds parathormone detection examination using the μ L of tissue eluent 90 extracted in pipettor aspiration step 2
The sample introduction zone of agent box.
The parathormone detection kit that tissue eluent is added in step 3 is inserted immune detector by step 4.
Detected detection port.
Further, the doubtful parathyroid gland described in step 2 still in the original location when, its organize eluent extraction step
For:The doubtful parathyroid gland of patient's Intraoperative position, its envelope is clipped broken with ophthalmic tweezers with teeth, and ophthalmic tweezers tip dips the tissue oozed out
Liquid, while operating nurse extracts 1mL physiological saline with 1mL syringes, is expelled in sterile vials;Patient will dip tissue fluid
Ophthalmic tweezers wash and wash in sterile vials, the tissue fluid adhered on tweezers is eluted, patient repeats this operation and washed to wash 3 times, obtains group
Knit eluent.
Further, the doubtful parathyroid gland described in step 2 still in the original location when, its organize eluent extraction step also
Can be:1mL physiological saline is extracted with 1mL syringes by operating nurse, is injected in sterile vials, while retaining in syringe
A small amount of physiological saline, then this syringe is punctured into doubtful Parathyroid Tissue, retreated using a small amount of tissue fluid of negative-pressure ward
Extract and be injected back into 2-3 times repeatedly in pin, the physiological saline that then needle point is immersed to sterile vials, fully to clean syringe, then
Liquid is all injected in sterile vials, tissue eluent is that making is finished.The preparation method of this tissue eluent we
It is named as pin and inhales method.
Further, though the doubtful parathyroid gland described in step 2 has been cut or observed in the original location without blood supply by mistake,
Its organize eluent extraction step be:Patient can be drawn off, as in vitro doubtful parathyroid gland;It is placed on kidney basin
One jiao, be cut into some with scalpel, operating nurse extracts 1mL physiology salts water droplet with 1mL syringes and drenched thereon, patient by it
Syringe pumpback is used in mixing again, is expelled in sterile vials, and tissue eluent is that making is finished.
Further, when in vitro doubtful parathyroid gland is cut into some, sheet should be cut into, it is so convenient in inspection
Result is surveyed to remedy directly to plant back after parathyroid gland.
Further, the detection pattern in step 1 is one kind in conventional detection pattern or quick detection pattern.
Further, when conventional detection pattern is have selected in step 1, parathormone detection kit is inserted in step 4
The step of detection port for entering immune detector is detected be:Patient information is inputted, parathormone detection kit is inserted
Enter the injection port of immune detector, instrument self-clocking, duration 12 minutes;Instrument automatic reading after 12 minutes;Taken according to this
Quadrat method is sampled, and root is it was found that this method the data obtained is more than 65pg/mL, you can confirm that this is organized as parathyroid gland.
Further, when have selected quick mode in step 1, parathormone Test paper is inserted immune in step 4
The step of detection port of detector is detected be:Parathormone detection kit is taken out and stood after 12 minutes, will
It is reading that parathormone detection kit, which is inserted after the detection mouth of immune detector, input patient information,;Gained reading is more than
65pg/mL, you can be judged as the positive;Quick detection pattern, which is applied to multiple tissues or position, to be needed to recognize the situation with identification.
Further, the parathormone detection kit described in step 3, including plastics cartridge and the first in box
Parathyrine Test paper by shape, plastics cartridge is provided with well, and parathormone Test paper includes bottom plate, sample pad, chemistry
Coupling pad, reacting pad and adsorptive pads, bottom plate are set to strip, and sample pad, chemical coupling pad, reacting pad and adsorptive pads are taken successively
Connect fixed be covered in above bottom plate;Sample pad is used to receive sample, and sample pad is arranged on below the well of plastics cartridge, step
Tissue eluent obtained by 2 instills sample pad by well;Gold labeling antibody conjugates, sample are coated with chemical coupling pad
Tissue eluent on product pad proceeds to chemical coupling pad under capillarity and gold labeling antibody conjugates are combined into and are immunized again
Compound;Reacting pad includes detection zone and quality control region, and detection zone is coated with parathormone first antibody in advance, in quality control region coating
There is goat-anti Streptavidin antibody, immune complex continues to be forwarded to the detection zone of reacting pad and parathormone first antibody is special
The opposite sex combines and is trapped to form detection line, and the complete immune complex of unreacted continues to be forwarded to the quality control region of reacting pad and sheep
Anti- Streptavidin antibody specificity combines to form nature controlling line.
Further, the bottom plate is set to PVC materials, and the sample pad is set to non-woven fabrics or glass fibre membrane, institute
State chemical coupling pad and be set to glass fibre membrane or polyester film, the reacting pad is set to nitrocellulose filter, the adsorptive pads
Absorbent filter is set to, the gold labeling antibody conjugates belong to the parathormone secondary antibody and collaurum mark of colloid gold label
The conjugates of the Streptavidin of note.
Parathormone detection kit disclosed in this invention uses solid-phase immunity Chromatographic techniques, utilizes double antibody
Sandwich method principle, the detection zone on test strip nitrocellulose filter is coated with parathormone first antibody in advance,
Quality control region is coated with goat-anti Streptavidin antibody, and the first of colloid gold label is coated with the chemical coupling pad of test strip
The conjugates of the Streptavidin of parathyrine secondary antibody and colloid gold label by shape.During detection, tissue eluent is added to first
In well, on the stage casing (53-68) of the thing parathormone polypeptide chain to be checked in tissue eluent and chemical coupling pad in advance
Coated gold labeling antibody conjugates combine to form immune complex, and the immune complex is chromatographed to inspection with the capillarity of film
Area is surveyed, is trapped with being coated on the parathormone first antibody generation specific binding (C-terminal is combined) of detection zone in advance,
Assemble and form detection line, unreacted immune complex continues to chromatograph to quality control region, with the goat-anti chain being coated in advance on film
Mould Avidin antibody specificity is combined, and forms nature controlling line.Within the specific limits, in the depth and measuring samples of detection line color
Parathyroid gland cellulose content proportional.After the completion of reaction, detection line and nature controlling line are scanned by immune detector
Analysis, and calculated according to the preset parameter of instrument internal, so that the parathyroid hormone in quantitative determination tissue eluent
Content.
Embodiment
In order that the object, technical solutions and advantages of the present invention are more clearly understood, with reference to embodiment and
Accompanying drawing, is described in further details to the present invention.Here, the present invention exemplary embodiment and its illustrate be used for explain this hair
It is bright but not as a limitation of the invention.
Brief description of the drawings:
Fig. 1, the principle schematic of parathormone detection.
Fig. 2, the structural representation of parathormone Test paper.
1st, bottom plate;2nd, sample pad;3rd, chemical coupling pad;4th, reacting pad;5th, adsorptive pads;6th, detection line;7th, nature controlling line.
1st, the embodiment of parathormone detection kit:Detected including plastics cartridge with the parathormone in box
Test paper, plastics cartridge is provided with well, and plastics cartridge is provided with the window for being able to observe that test paper, parathormone inspection
Test paper includes bottom plate, sample pad, chemical coupling pad, reacting pad and adsorptive pads, and bottom plate is set to strip, sample pad, chemistry
Coupling pad, reacting pad and adsorptive pads overlap fixed be covered in above bottom plate successively;Sample pad is used to receive sample, and sample pad is set
Below the well of plastics cartridge, resulting tissue eluent instills sample pad by well in step 2;Chemical coupling
The tissue eluent being coated with pad in gold labeling antibody conjugates, sample pad proceeds to chemical coupling pad and gold labeling antibody conjugates
It is combined into immune complex;Reacting pad includes detection zone and quality control region, and detection zone is coated with parathormone first antibody in advance,
Goat-anti Streptavidin antibody is coated with quality control region, immune complex continues to be forwarded to the detection zone of reacting pad and parathyroid gland
Plain first antibody specifically binds and is trapped to form detection line, and the complete immune complex of unreacted continues to be forwarded to reacting pad
Quality control region combined with goat-anti Streptavidin antibody specificity and obtain nature controlling line.
Further, the bottom plate is set to PVC materials, and the sample pad is set to non-woven fabrics or glass fibre membrane, institute
State chemical coupling pad and be set to glass fibre membrane or polyester film, the reacting pad is set to nitrocellulose filter, the adsorptive pads
Absorbent filter is set to, the gold labeling antibody conjugates are specially the parathormone secondary antibody and collaurum of colloid gold label
The conjugates of the Streptavidin of mark.
Parathormone detection kit disclosed in this invention uses solid-phase immunity Chromatographic techniques, utilizes double antibody
Sandwich method principle, the detection zone on test strip nitrocellulose filter is coated with parathormone first antibody in advance,
Quality control region is coated with goat-anti Streptavidin antibody, and the first of colloid gold label is coated with the chemical coupling pad of test strip
The conjugates of the Streptavidin of parathyrine secondary antibody and colloid gold label by shape.During detection, tissue eluent is added to first
In well, on the stage casing (53-68) of the thing parathormone polypeptide chain to be checked in tissue eluent and chemical coupling pad in advance
Coated gold labeling antibody conjugates combine to form immune complex, and the immune complex is chromatographed to inspection with the capillarity of film
Area is surveyed, is cut with being coated on the parathormone first antibody generation specific binding (C-terminal is combined) of detection zone in advance
Stay, assemble and form detection line, unreacted immune complex continues to chromatograph to quality control region, with the goat-anti being coated in advance on film
Streptavidin antibody specificity is combined, and forms nature controlling line.Within the specific limits, in the depth and measuring samples of detection line color
Parathormone content proportional.After the completion of reaction, detection line and nature controlling line are carried out by immune detector
Scanning analysis, and calculated according to the preset parameter of instrument internal, so that the parathyroid gland in quantitative determination tissue eluent
Hormone-content.
Parathormone detection kit has used immune colloidal gold technique, is to be used as tracer label substance markers using collaurum
A kind of new immunolabelling technique of antibody.Collaurum (colloidal gold) is also referred to as aurosol, is reduced by gold salt
The gold grain suspension of Cheng Jinhou formation.Ion layer of the colloid gold particle by the golden core (atom gold Au) in a basis and encirclement outside
Constitute, that close to golden core surface is internal layer anion (AuC12-), outer layer sheath H+ is then dispersed between colloid in solution,
Intergranular electrostatic repulsion forces make collaurum be free between colloidal sol, form suspension.Colloid gold label, substantially protein etc.
Macromolecule is adsorbed to the coating process on colloid gold particle surface.Colloid gold particle has very strong adsorption function to protein, and
Not by its bad bioactivity, glue can be formed with the Non-covalent binding such as protein etc. (staphylococcal protein A, immunoglobulin)
Body gold label.The tracer material that external fast diagnosis reagent is used is mainly Au colloidal nanoparticles, and colloid gold particle
Size and dispersiveness whether equal a pair of reagent manufactures detection sensitivity and accuracy produce very big influence.Traditional
Colloid gold particle preparation method obtain colloid gold particle color tend to appear as aubergine, size mainly in 20-40nm or so,
Dispersiveness is uneven, so as to cause the detection sensitivity of reagent manufacture relatively low, accuracy and difference between batch are than larger.And at this
Its granular size of parathyroid gland (parathormone) detection reagent being related in new application is 100nm or so, and with good
Dispersiveness and homogeneity, antibody is coupled using the bulky grain collaurum, the detection sensitivity of product is greatly improved
And accuracy.
2nd, immune detector is used for the embodiment of the conventional detection pattern for the application method that parathyroid gland is recognized:
Step 1. is started shooting to be checked
One hundred immune detector difficult to understand that reaches is started shooting, conventional detection pattern is clicked with stylus, it is to be checked.
Step 2. organizes the making of eluent
Gripping method:Doubtful parathyroid gland in the original location when, organize eluent acquisition and making:The doubtful first of patient's Intraoperative position
Gland by shape, its envelope is clipped broken with ophthalmic tweezers with teeth, ophthalmic tweezers tip dips the tissue fluid oozed out, while operating nurse is noted with 1mL
Emitter extracts 1mL physiological saline, is expelled in sterile vials.Patient washes the ophthalmic tweezers for having dipped tissue fluid in sterile vials
Wash, the tissue fluid adhered on tweezers is eluted, patient repeats this operation and washes and wash 3 times, obtains tissue eluent.
Pin inhales method:1mL physiological saline is extracted with 1mL syringes by operating nurse, is injected in sterile vials, injects simultaneously
Retain a small amount of physiological saline in device, then this syringe is punctured into doubtful Parathyroid Tissue, utilize a small amount of group of negative-pressure ward
The withdraw of the needle after liquid is knitted, extracts and is injected back into 2-3 times repeatedly in the physiological saline that then needle point is immersed to sterile vials, with abundant cleaning needle
Liquid, is then all injected in sterile vials by cylinder, and tissue eluent is that making is finished.The making side of this tissue eluent
Method we by it be named as pin inhale method.
Suspension method:Though if doubtful parathyroid gland cut by mistake or in the original location but observe without blood supply, patient can be taken
Go out, as in vitro doubtful parathyroid gland.One jiao of kidney basin is placed on, some is cut into scalpel, operating nurse is used
1mL syringes extract 1mL physiology salts water droplets and drenched thereon, patient by mixing use syringe pumpback again, be expelled in sterile vials,
It is that making is finished to organize eluent.Explanation:When doubtful other glandular tissue is cut into some, sheet, detection knot are preferably cut into
Fruit is remedied directly to be planted back after parathyroid gland.
Step 3. is sampled, sample introduction opens parathyroid gland detection kit packaging, is taken out kit liquid-transfering gun and is connected pipette tips, makes
The above-mentioned μ L of tissue eluent 90 are taken with pipettor, tissue eluent is added into parathyroid gland detection kit from sample holes.
Step 4. is detected
By the detection port of parathyroid gland Test paper inserting instrument, patient information, instrument self-clocking, duration 12 are inputted
Minute.Instrument automatic reading after 12 minutes.According to the data of our clinical tests, result and frozen section pathology knot are finally examined to obtain
Fruit verifies that this method the data obtained is more than 65pg/mL, when, frozen section pathological examination is parathyroid gland.
3rd, immune detector is used for the embodiment of the quick detection pattern for the application method that parathyroid gland is recognized:
Step 1. is started shooting to be checked
One hundred immune detector difficult to understand that reaches is started shooting, quick detection pattern is clicked with stylus, it is to be checked.
Step 2. organizes the making of eluent
Gripping method:The doubtful parathyroid gland of patient's Intraoperative position, its envelope is clipped broken with ophthalmic tweezers with teeth, and ophthalmic tweezers tip is dipped
The tissue fluid oozed out, while operating nurse extracts 1mL physiological saline with 1mL syringes, is expelled in sterile vials.Patient will
The ophthalmic tweezers for having dipped tissue fluid are washed in sterile vials and washed, and the tissue fluid adhered on tweezers is eluted, and patient repeats this operation
Wash and wash 3 times, obtain tissue eluent, the preparation method of this tissue eluent we be named as gripping method.
Pin inhales method:1mL physiological saline is extracted with 1mL syringes by operating nurse, is expelled in sterile vials.Patient is with together
One syringe, draws physiological saline a little, then this syringe is punctured into doubtful Parathyroid Tissue, utilizes negative-pressure ward
A small amount of tissue fluid, syringe is exited in vitro, is then immersed needle point and is extracted, is injected back into repeatedly in the sterile vials equipped with physiological saline
Liquid is all injected in sterile vials after physiology 2-3 times, fully cleaning syringe, tissue eluent is that making is finished.
Suspension method:Though if doubtful parathyroid gland cut by mistake or in the original location but observe without blood supply, patient can be taken
Go out, as in vitro doubtful parathyroid gland.Sterile one jiao of kidney basin is placed on, some, operation shield are cut into scalpel
Scholar extracts 1mL physiology salts water droplets with 1mL syringes and drenched thereon, patient by mixing use syringe pumpback again, be injected into sterile small
In bottle, tissue eluent is that making is finished.Explanation:When doubtful other glandular tissue is cut into some, sheet is preferably cut into, really
Recognize directly to plant back after the positive and remedy.
Step 3. takes the above-mentioned μ L of tissue eluent 90 using pipettor, is dripped from the sample holes of parathormone detection kit
To parathormone Test paper, side is put, incubation time -12 minutes 3 minutes.
Step 4. quick detection
It is reading that parathormone Test paper, which is inserted after detection mouth, input patient information,.Finally examine to obtain result and ice
Freeze section pathological examination and verify that this method the data obtained is more than 65pg/mL, be positive (confirming it is parathyroid gland).Quickly
Detection pattern, which is applied to multiple detection positions, to be needed to detect or only need situation about just slightly judging.
Parathyroid gland is identified using immune detector, detection can be completed in 12 minutes, and using quick
Multiple detection positions can be detected by detection pattern simultaneously, and simple to operate, and cost is relatively low, and its principle is to tissue
Whether parathyroid hormone levels are detected in eluent, be parathyroid gland according to concentration results intuitive judgment suspect tissue,
As a result it is more accurate.
Claims (10)
1. a kind of new application of immune detector in operation, it is characterised in that:Use it for the identification of parathyroid gland.
2. a kind of immune detector is used for the application method of new application, its step is as follows:
Step 1. is started shooting, and selects detection pattern, to be checked.
Step 2. extracts the tissue eluent of doubtful parathyroid gland.
The tissue eluent extracted in step 2 is instilled parathormone detection kit by step 3..
Step 4. inserts the parathormone detection kit that tissue eluent has been instilled in step 3 detection of immune detector
Detected port.
3. a kind of immune detector as claimed in claim 2 is used for the application method of new application, it is characterised in that in step 2
Described doubtful parathyroid gland still in the original location when, its organize eluent extraction step be:By the doubtful first shape of patient's Intraoperative position
Gland, its envelope is clipped broken with ophthalmic tweezers with teeth, and ophthalmic tweezers tip dips the tissue fluid oozed out, while operating nurse 1mL syringes
1mL physiological saline is extracted, is expelled in sterile vials;Patient washes the ophthalmic tweezers for having dipped tissue fluid in sterile vials to wash,
By the tissue fluid adhered on tweezers elution, patient repeats this operation and washes and wash 3 times, obtains tissue eluent.
4. a kind of immune detector as claimed in claim 2 is used for the application method of new application, it is characterised in that in step 2
Described doubtful parathyroid gland still in the original location when, its organize eluent extraction step can also be:Noted by operating nurse with 1mL
Emitter extracts 1mL physiological saline, is injected in sterile vials, while retaining a small amount of physiological saline in syringe, then this is injected
Device is punctured into doubtful Parathyroid Tissue, and using the withdraw of the needle after a small amount of tissue fluid of negative-pressure ward, needle point then is immersed into sterile small
Extract and be injected back into 2-3 times repeatedly in the physiological saline of bottle, fully to clean syringe, liquid is all then injected to sterile vials
In, tissue eluent is that making is finished.
5. a kind of immune detector as claimed in claim 2 is used for the application method of new application, it is characterised in that in step 2
Though described doubtful parathyroid gland cut by mistake or in the original location observe without blood supply when, its organize eluent extraction step
For:Patient can be drawn off, as in vitro doubtful parathyroid gland;One jiao of kidney basin is placed on, sheet is cut into scalpel,
Operating nurse extracts 1mL physiology salts water droplets with 1mL syringes and drenched thereon, patient by mixing use syringe pumpback again, be expelled to
In sterile vials, tissue eluent is that making is finished.
6. a kind of immune detector as claimed in claim 2 is used for the application method of new application, it is characterised in that in step 1
Detection pattern be one kind in conventional detection pattern or quick detection pattern.
7. a kind of immune detector as claimed in claim 6 is used for the application method of new application, it is characterised in that in step 1
When have selected conventional detection pattern, the detection port that parathormone detection kit is inserted into immune detector in step 4 is entered
Row detection the step of be:Patient information is inputted, parathormone detection kit is inserted to the detection mouth of immune detector, instrument
Self-clocking, duration instrument automatic reading after 12 minutes, 12 minutes.
8. a kind of immune detector as claimed in claim 6 is used for the application method of new application, it is characterised in that in step 1
When have selected quick detection pattern, the detection port that parathormone detection kit is inserted into immune detector in step 4 is entered
Row detection the step of be:Parathormone detection kit is taken out and stood after 3-12 minutes, parathormone is detected and tried
It is reading that agent box, which is inserted after the detection mouth of immune detector, input patient information,.
9. a kind of immune detector as claimed in claim 2 is used for the application method of new application, it is characterised in that the first shape
Other parathyrine detection kit includes plastics cartridge and the parathormone Test paper in box, and plastics cartridge is provided with sample-adding
Hole, and plastics cartridge is provided with the window for being able to observe that test paper, parathormone Test paper include bottom plate, sample pad,
Chemical coupling pad, reacting pad and adsorptive pads, bottom plate are set to strip, sample pad, chemical coupling pad, reacting pad and adsorptive pads according to
Secondary overlap joint is fixed to be covered in above bottom plate;Sample pad is used to receive sample, and sample pad is arranged on below the well of plastics cartridge;
Gold labeling antibody conjugates are coated with chemical coupling pad;Reacting pad includes detection zone and quality control region, and detection zone is coated with first in advance
Parathyrine first antibody by shape, goat-anti Streptavidin antibody is coated with quality control region.
10. a kind of immune detector as claimed in claim 9 is used for the application method of new application, it is characterised in that the bottom
Plate is set to PVC materials, and the reacting pad is set to nitrocellulose filter, and the gold labeling antibody conjugates are set to collaurum mark
The conjugates of the parathormone secondary antibody of note and the Streptavidin of colloid gold label.
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