CN107213165A

CN107213165A - Placenta hydrolysis ultrafiltrate and preparation method thereof, using and feminine care gel

Info

Publication number: CN107213165A
Application number: CN201710514675.1A
Authority: CN
Inventors: 蔡豪斌
Original assignee: Foshan Nanhai Pacific Biotechnology Co Ltd
Current assignee: Foshan Nanhai Pacific Biotechnology Co Ltd
Priority date: 2017-06-29
Filing date: 2017-06-29
Publication date: 2017-09-29

Abstract

The present invention provide placenta hydrolysis ultrafiltrate and preparation method thereof, using and feminine care gel.The feminine care gel using placenta by hydrolyzing ultrafiltrate as the primary raw material of the feminine care gel, and placenta hydrolysis ultrafiltrate is prepared using special process, molecular weight is in 1KD to 10KD, placenta hydrolysis ultrafiltrate effectively eliminates free hormone of the molecular weight in below 1KD, will not cause " knock-on of being discontinued "；And rich in various small molecule active ingredient such as growth stimulations and regulatory factor, by giving female genital local application, permucosal absorption enters blood, quickly reaches ovary, there is special effect to recovering gonad cell vigor, stimulating gonad cell to regenerate；In addition, being obviously improved women's ovary various functions, delay the decline of ovarian function, correct the endocrine function of women imbalance, make the body and mind of women younger, more healthy.

Description

Placenta hydrolysis ultrafiltrate and preparation method thereof, using and feminine care gel

Technical field

The present invention relates to biological technical field, and more particularly to a kind of placenta hydrolyzes ultrafiltrate and preparation method thereof, answered With with feminine care gel.

Background technology

With advancing age, ovarian function fall off generally since 28 years old, adjoint is that skin holds to adult female The aging gradually of face and phychology.The oral and various sex hormone preparations of injection can substitute the ovarian function of decline, but it is to ovum The suppression of nest function causes serious " knock-on of being discontinued ", is disabled comprehensively at present.In addition, oral some similar sex hormone point The natural extract such as phytosterol of son, although have certain effect to the decline for delaying ovarian function, but due to phytosterol point The difference of minor structure, destruction of the digestive system to active ingredient during adding orally is acted on extremely limited.

Therefore prior art has yet to be improved and developed.

The content of the invention

In view of this, the present invention provide a kind of placenta hydrolysis ultrafiltrate and preparation method thereof, using and feminine care gel, Aim to solve the problem that and cause interference with human normal endocrine function by the oral or various sex hormone preparations of injection in the prior art Potential hazard.

The technical proposal for solving the technical problem of the invention is as follows：

A kind of placenta hydrolyzes the preparation method of ultrafiltrate, comprises the following steps：

Step 1, fresh or freezing placenta is weighed, in rejecting manadesma and big blood vessel under cleaning sterile environment, at 4 DEG C -10 DEG C sterile frozen water in rinse well, obtain clean placenta；

Step 2, the cleaning placenta obtained by step 1 is cut into small pieces, rubbed in bruisher, obtain crushing placenta；

Step 3, added in placenta and heavy small such as fresh or freezing placenta in step 1 to crushing obtained by step 2 Micel water, is positioned in sterile stainless steel ware, sealing, in -20 DEG C or less of temperature it is quick-frozen overnight, be subsequently placed in 4 DEG C - Melt completely in the environment of 10 DEG C, it is repeatedly quick-frozen with melting operation repeatedly, obtain hydrolyzate；

Step 4, the hydrolyzate obtained in step 3 is carried out in centrifuge to centrifugation -60 minutes 30 minutes, obtain supernatant Liquid；Wherein, the centrifugal speed of the centrifuge is at least 3500RPM；

Step 5, the supernatant for obtaining step 4 obtain coarse filtration liquid after qualitative filter paper suction filtration, and coarse filtration liquid is surpassed from 10KD Filter in post after ultrafiltration, abandon and penetrate liquid and stay omission timber, obtain the omission timber that molecular weight is less than 10KD；The omission timber is surpassed from 1KD Filter in post after ultrafiltration, abandon omission timber and stay and penetrate liquid, obtain ultrafiltrate of the molecular weight in 1KD-10KD.

Described placenta hydrolyzes the preparation method of ultrafiltrate, wherein, the freezing placenta in the step 1 need in advance 4 DEG C- Thawed completely in the environment of 10 DEG C.

A kind of placenta hydrolyzes ultrafiltrate, and the preparation method for hydrolyzing ultrafiltrate using placenta as described above is prepared.

A kind of placenta as described above hydrolyzes the application of ultrafiltrate, and the placenta hydrolysis ultrafiltrate is preparing skin care item, female Application in sexual reproduction road partial decontamination product or women ovary care cosmetic product.

A kind of feminine care gel, its component includes placenta hydrolysis ultrafiltrate, small-micelle water, NMF, skin condition Agent, thickener, pH adjusting agent and freshener, the placenta hydrolysis ultrafiltrate are that placenta as described above hydrolyzes ultrafiltrate.

Described feminine care gel, wherein, according to weight/mass percentage composition of each component in the feminine care gel Meter, including：

The placenta hydrolyzes ultrafiltrate, and content is 30%~70%；

The small-micelle water, content is 10%~50%；

The NMF, content is 8%~20%；

The skin conditioning agent, content is 6%~9%；

The thickener, content is 1%~3%；

The pH adjusting agent, content is 0.05%~0.15%；

The freshener, content is 0.02%~0.2%.

Described feminine care gel, wherein, the NMF be glycerine, propane diols or butanediol in one kind or Two or more mixtures.

Described feminine care gel, wherein, the skin conditioning agent is glycerine polymethacrylates, ethanol, hydrogen-oxygen Change ammonium, n- butanol, isopropanol, edelweiss floral leaf extract, kuh-seng root extract, houttuynia extract, hydrolytic collagen or One or more kinds of mixtures in person's hyaluronic acid.

Described feminine care gel, wherein, the thickener is that hydroxyethyl cellulose or Sodium Polyacrylate grafting are formed sediment The mixture of one or two kinds of in powder.

A kind of preparation method of feminine care gel as described above, comprises the following steps：

Step 1, placenta is hydrolyzed into ultrafiltrate, skin conditioning agent, freshener be added to successively in small-micelle water, to complete Dissolving, it is well mixed to obtain the first mixture；

Step 2, NMF, thickener be added sequentially in the first mixture in step 1, be completely dissolved it, mix Conjunction uniformly obtains the second mixture；

Step 3, pH adjusting agent is added in the second mixture, obtains the feminine care gel.

Compared with prior art, the present invention has advantages below：

Adopted by the way that placenta to be hydrolyzed to ultrafiltrate as the primary raw material of the feminine care gel, and placenta hydrolysis ultrafiltrate Prepared with special process, molecular weight effectively eliminates molecular weight below 1KD's in 1KD to 10KD, placenta hydrolysis ultrafiltrate Free hormone, will not cause " knock-on of being discontinued "；And rich in various small molecule active ingredient such as growth stimulations and regulatory factor, pass through Female genital local application is given, permucosal absorption enters blood, quickly reaches ovary, to recovering gonad cell vigor, stimulating ovary Cytothesis has special effect；In addition, being obviously improved women's ovary various functions, delay the decline of ovarian function, correct women The endocrine function of imbalance, makes the body and mind of women younger, more healthy.

Embodiment

To make the objects, technical solutions and advantages of the present invention clearer, clear and definite, the present invention is entered with reference to embodiments One step is described in detail.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to limit this Invention.

The invention provides the preparation method that a kind of placenta hydrolyzes ultrafiltrate, comprise the following steps：

Step 3, the pH to weights such as fresh or freezing placentas crushed in placenta in addition and step 1 obtained by step 2 Small-micelle water more than 9.6, is positioned in sterile stainless steel ware, sealing, quick-frozen overnight in -20 DEG C or less of temperature, Melt completely in the environment of being subsequently placed in 4 DEG C -10 DEG C, it is repeatedly quick-frozen with melting operation repeatedly, obtain hydrolyzate；

Wherein, the freezing placenta in step 1 needs to thaw completely in the environment of 4 DEG C -10 DEG C in advance.

The granularity of the crushing placenta obtained in the step 2 is the mesh of 60 mesh -120.

Several embodiments of the preparation method of placenta hydrolysis ultrafiltrate of the present invention are given below.

Embodiment 1

Step 1, the fresh human placenta for weighing people, in rejecting manadesma and big blood vessel under cleaning sterile environment, in 4 DEG C of sterile ice Rinsed well in water, obtain cleaning placenta；Wherein, the granularity for crushing placenta is 60 mesh；

Step 3, to be added to crushing obtained by step 2 in placenta with the pH of the weight such as the fresh human placenta in step 1 be 12.5 Small-micelle water, is positioned in sterile stainless steel ware, sealing, in the quick-frozen ring for being subsequently placed in 4 DEG C overnight of -20 DEG C of temperature Melt completely under border, it is repeatedly quick-frozen with melting operation 3 times, obtain hydrolyzate；

Wherein, the small-micelle water is to be concluded to form by 3-6 hydrone, and its movement velocity is fast, is oozed with extremely strong Saturating power, extension, dissolving power.The pH value of small-micelle water is higher, and the efficiency of hydrolysis is higher.

Step 4, the hydrolyzate obtained in step 3 is carried out in centrifuge to centrifugation 30 minutes, obtain supernatant；Wherein, The centrifugal speed of the centrifuge is 3500RPM；

Step 5, the supernatant for obtaining step 4 obtain filtrate after qualitative filter paper suction filtration, by filtrate from 10KD ultrafiltration posts After middle filtering, the omission timber that molecular weight is less than 10KD is obtained；After the omission timber is filtered from 1KD ultrafiltration posts, molecule is obtained Measure and hydrolyze ultrafiltrate for 1KD-10KD placenta；

Embodiment 2

Step 1, the freezing placenta for weighing animal, thaw, then in cleaning sterile environment completely in the environment of 4 DEG C in advance Lower rejecting manadesma and big blood vessel, are rinsed well in 10 DEG C of sterile frozen water, obtain cleaning placenta；

Step 2, the cleaning placenta obtained by step 1 is cut into small pieces, rubbed in bruisher, obtain crushing placenta；Wherein, The granularity for crushing placenta is 120 mesh；

Step 3, to crushing obtained by step 2 added in placenta with the pH of the weight such as the freezing placenta in step 1 be 10 it is small Micel water, is positioned in sterile stainless steel ware, sealing, in the quick-frozen environment for being subsequently placed in 7 DEG C overnight of -25 DEG C of temperature It is lower to melt completely, it is repeatedly quick-frozen with melting operation 4 times, obtain hydrolyzate；

Step 4, the hydrolyzate obtained in step 3 is carried out in centrifuge to centrifugation 45 minutes, obtain supernatant；Wherein, The centrifugal speed of the centrifuge is 4000RPM；

Embodiment 3

Step 1, the freezing placenta for weighing animal, thaw, then in cleaning sterile ring completely in the environment of 10 DEG C in advance Manadesma and big blood vessel are rejected under border, is rinsed well in 5 DEG C of sterile frozen water, obtains cleaning placenta；

Step 2, the cleaning placenta obtained by step 1 is cut into small pieces, rubbed in bruisher, obtain crushing placenta；Wherein, The granularity for crushing placenta is 80 mesh；

Step 3, to crushing obtained by step 2 added in placenta with the pH of the weight such as the freezing placenta in step 1 be 9.6 it is small Micel water, is positioned in sterile stainless steel ware, sealing, in the quick-frozen environment for being subsequently placed in 10 DEG C overnight of -30 DEG C of temperature It is lower to melt completely, it is repeatedly quick-frozen with melting operation 5 times, obtain hydrolyzate；

Step 4, the hydrolyzate obtained in step 3 is carried out in centrifuge to centrifugation 60 minutes, obtain supernatant；Wherein, The centrifugal speed of the centrifuge is 5000RPM；

Ultrafiltrate is hydrolyzed present invention also offers a kind of placenta, the preparation side of ultrafiltrate is hydrolyzed using placenta as described above Method is prepared.

The biology that the placenta hydrolysis ultrafiltrate of the present invention can strongly hydrolyze placenta by highly polar small-micelle water is big Molecule, it is to avoid because the way that is hydrolyzed using strong acid and strong base causes to introduce pollutant and other has noxa in conventional art Son；In combination with -20 DEG C and 4 DEG C of repeated multiple times freeze thawing, it is to avoid destruction of the high temperature to bioactive substance, to greatest extent Ground remains the activity of biomolecule in placenta；And molecular sieve retention operation is carried out with the use of two ultrafiltration posts of 10KD and 1KD, So that the placenta hydrolysis ultrafiltrate finally given remains the bioactivity of placenta small molecular material as much as possible, while pick again Except the hormonal components dissociated in placenta, the potential hazard that free hormone disturbs human normal endocrine function is eliminated.

A kind of 40 glucocorticoid test experiences are done to the placenta hydrolysis ultrafiltrate of the present invention, to prove it without any A kind of cortin, 40 species of glucocorticoid are shown in Table 1.

Table 1

A kind of 40 glucocorticoids

Using GB/T24800.2-2009, the assay method of liquid chromatogram/tandem mass spectrometry is hydrolyzed to the placenta of the present invention Ultrafiltrate does a kind of 40 glucocorticoid test experiences, as a result shows the 40 a kind of sugared cortex not detected shown in table 1 Hormone.

It follows that placenta hydrolysis ultrafiltrate prepared by the present invention is free of any cortin, long-term use will not be injured Skin Cell.

The invention provides the application that a kind of placenta hydrolyzes ultrafiltrate, placenta hydrolysis ultrafiltrate prepare skin care item, Application in female genital tract partial decontamination product or women ovary care cosmetic product.

Present invention also offers a kind of feminine care gel, its component includes placenta hydrolysis ultrafiltrate, small-micelle water, guarantor Humectant, skin conditioning agent, thickener, pH adjusting agent and freshener, the placenta hydrolysis ultrafiltrate is above-mentioned specific embodiment Described in placenta hydrolysis ultrafiltrate, will not be repeated here.

Wherein, the placenta hydrolyzes ultrafiltrate as the primary raw material (ovary care agent) of the feminine care gel, and it is adopted Prepared with special process, molecular weight effectively eliminates molecular weight below 1KD's in 1KD to 10KD, placenta hydrolysis ultrafiltrate Free hormone, will not cause " knock-on of being discontinued "；And rich in various small molecule active ingredient such as growth stimulations and regulatory factor, pass through Female genital local application is given, permucosal absorption enters blood, quickly reaches ovary, to recovering gonad cell vigor, stimulating ovary Cytothesis has special effect；In addition, being obviously improved women's ovary various functions, delay the decline of ovarian function, correct women The endocrine function of imbalance, makes the body and mind of women younger, more healthy.

The feminine care gel application prepared with the present invention is in trier, with the work(for the feminine care gel for proving the present invention Effect, concrete operations are as follows：

1st, volunteer's selection is tried out

This totally 119 volunteer on probation participates in, and is married, age 31-57 Sui (median 47.5 years old), before trying out Sign informed consent form.Two cycle persons 31 are tried out in selection a cycle person 27 on probation, selection, and three cycles are tried out in selection Person 61.All 119 volunteers, which are both needed to submit, tries out front and rear blood serum E2 checklist, and completes " trial effect experience investigation Table " is filled in.

2nd, the selection of gel is tried out

From the feminine care gel of the present invention

3rd, trial method

(1) all triers set up archives, state clearly age, menstrual history and obsterical history；

(2) all triers adopted venous blood in the 7th day after clean period, sent local Grade A hospital to examine serum estradiol E2 Level；

(3) all triers started gel on probation in the 8th day after clean period, can independently select every 1 day with once or Every 2 days with once, each consumption 1 (5mL) is disabled as a cycle to menstrual period；

(4) it is finished what is voluntarily stopped using after a cycle, venous blood was adopted in the 7th day after this clean period, send and work as Ground Grade A hospital examines serum estradiol E2 levels；

(5) it is finished after a cycle and voluntarily continues what is tried out, continued gel on probation in the 8th day after this clean period, together Sample with a cycle every 1 day with once or every 2 days with once (being consistent), and each consumption 1 (5mL) is stopped to menstrual period With for second period；

(6) it is finished what is voluntarily stopped using after second period, venous blood was adopted in the 7th day after this clean period, send and work as Ground Grade A hospital examines serum estradiol E2 levels；

(7) it is finished after second period and voluntarily continues what is tried out, continued gel on probation in the 8th day after this clean period, It is same every 1 day with once or every 2 days with once (being consistent with a cycle), each consumption 1 (5mL), to menstrual period Disable as the 3rd cycle；

(8) it is finished after the 3rd cycle and adopted venous blood in the 7th day after this clean period, send local Grade A hospital to examine Serum estradiol E2 levels；

(9) a cycle has often been tried out, trier has been both needed to and fills in " trial effect experience application form " as shown in table 2：

Table 2

Ovary nursing gel trial effect experiences application form

4. result statistics, analysis

(1) ordinary circumstance explanation is tried out

This all 119 volunteer, which have submitted, tries out front and rear blood serum E2 checklist, and completes " trial effect Experience application form " fill in.

(2) statistics of serum estrogen levels, analysis before and after trying out

By trier on probation 1 month, 2 months and 3 months, the value of blood serum E2 level is done with contrast before and after gel on probation Compared with as a result as shown in table 3：

Table 3

The value of trier's blood serum E2 level before and after gel on probation

As shown in Table 3, it can be seen that blood serum E2 has different degrees of raising after gel on probation.Try out 1 month person 77.8% Trier's blood serum E2 level significantly improve, try out 2 months persons 90.3%, try out 3 months persons up to 95.1%.

(3) statistics of trier's usage experience, analysis

By the trier of 1 month, 2 months and 3 months on probation before and after gel on probation color spot desalination, wrinkle reduce, moisten, More smooth, more flexible and gloss value does paired comparisons, as a result as shown in table 4：

Table 4

As shown in Table 4, it can be seen that trier's effect is most fast, most obvious experience is " moist ", next to that " it is more smooth, More flexible and gloss ", they reach 100% in the experience for trying out three months persons.The experience of " wrinkle reduction " is on probation 2 I.e. up to 64.5%, three month up to 80.3% after month.The experience of " color spot desalination " reached 58.1% at 2 months, reached within 3 months 65.6%.

Because blood serum E2 level is the mark of ovarian function, its main function is to maintain menstrual cycle and women secondary Levy, the raising of its level can make female skin tenderer whiter more flexible, and phychology is younger.It is to sum up shown, it can be seen that this The blood serum E2 level change of investigation on probation has positive correlation with trier's experience, illustrate that feminine care gel of the invention can be with Ovarian function is significantly heightened, recovers adult female's secondary sex characters.

It is preferred that, according to weight/mass percentage composition meter of each component in the feminine care gel, including：

The placenta hydrolyzes ultrafiltrate, and content is 30%~70%；

The small-micelle water, content is 10%~50%；

The NMF, content is 8%~20%；

The skin conditioning agent, content is 6%~9%；

The thickener, content is 1%~3%；

The pH adjusting agent, content is 0.05%~0.15%；

The freshener, content is 0.02%~0.2%.

It is preferred that, the NMF is one or more kinds of mixtures in glycerine, propane diols or butanediol. The skin conditioning agent is glycerine polymethacrylates, ethanol, ammonium hydroxide, n- butanol, isopropanol, edelweiss floral leaf It is one or more kinds of mixed in extract, kuh-seng root extract, houttuynia extract, hydrolytic collagen or hyaluronic acid Compound.

It is preferred that, the thickener is the one or two kinds of in hydroxyethyl cellulose or Sodium Polyacrylate graft starch Mixture.The freshener is borneol or the mixture of the one or two kinds of in menthol；The pH adjusting agent is lemon Lemon acid.

It is preferred that, the pH value of the small-micelle water is 9.5.

The preparation method of the feminine care gel of the present invention, comprises the following steps：

Further details of retouch is done to component, the preparation method of feminine care gel of the present invention with four embodiments below State.

Embodiment 1

Feminine care gel in the present embodiment, according to weight/mass percentage composition of each component in the feminine care gel Meter, including：

Placenta hydrolyzes ultrafiltrate, and content is 30%；

Small-micelle water, content is 37.65%；

NMF, content is 20%；

Skin conditioning agent, content is 9%；

Thickener, content is 3%；

PH adjusting agent, content is 0.15%；

Freshener, content is 0.2%.

The preparation method of feminine care gel in the present embodiment is as follows：

It is 0.2% that step 1, the placenta for being 30% by content, which hydrolyze ultrafiltrate, the skin conditioning agent that content is 9%, content, Freshener be added to successively in the small-micelle water that content is 37.65%, it is well mixed to obtain the first mixing to being completely dissolved Thing；

What the thickener that step 2, the NMF for being 20% by content, content are 3% was added sequentially in step 1 first mixes In compound, it is completely dissolved it, it is well mixed to obtain the second mixture；

Step 3, content is added in the second mixture for 0.15% pH adjusting agent, obtains the feminine care and coagulate Glue.

Embodiment 2

Placenta hydrolyzes ultrafiltrate, and content is 70%；

Small-micelle water, content is 10%；

NMF, content is 12.93%；

Skin conditioning agent, content is 6%；

Thickener, content is 1%；

PH adjusting agent, content is 0.05%；

Freshener, content is 0.02%.

It is 0.02% that step 1, the placenta for being 70% by content, which hydrolyze ultrafiltrate, the skin conditioning agent that content is 6%, content, Freshener be added to successively in the small-micelle water that content is 10%, it is well mixed to obtain the first mixture to being completely dissolved；

The thickener that step 2, the NMF for being 12.93% by content, content are 1% is added sequentially in step 1 In one mixture, it is completely dissolved it, it is well mixed to obtain the second mixture；

Step 3, content is added in the second mixture for 0.05% pH adjusting agent, obtains the feminine care and coagulate Glue.

Embodiment 3

Placenta hydrolyzes ultrafiltrate, and content is 34.8%；

Small-micelle water, content is 50%；

NMF, content is 8%；

Skin conditioning agent, content is 6%；

Thickener, content is 1%；

PH adjusting agent, content is 0.1%；

Freshener, content is 0.1%.

Step 1, the placenta for being 34.8% by content hydrolyze ultrafiltrate, the skin conditioning agent that content is 6%, content 0.1% freshener is added in the small-micelle water that content is 50% successively, to being completely dissolved, well mixed to obtain first and mix Compound；

What the thickener that step 2, the NMF for being 8% by content, content are 1% was added sequentially in step 1 first mixes In compound, it is completely dissolved it, it is well mixed to obtain the second mixture；

Step 3, content is added in the second mixture for 0.1% pH adjusting agent, obtains the feminine care gel.

Embodiment 4

Placenta hydrolyzes ultrafiltrate, and content is 50%；

Small-micelle water, content is 24.23%；

NMF, content is 16%；

Skin conditioning agent, content is 7.5%；

Thickener, content is 2%；

PH adjusting agent, content is 0.12%；

Freshener, content is 0.15%.

Step 1, the placenta for being 50% by content hydrolyze ultrafiltrate, the skin conditioning agent that content is 7.5%, content 0.15% freshener is added in the small-micelle water that content is 24.23% successively, to being completely dissolved, well mixed to obtain the One mixture；

What the thickener that step 2, the NMF for being 16% by content, content are 2% was added sequentially in step 1 first mixes In compound, it is completely dissolved it, it is well mixed to obtain the second mixture；

Compared with prior art, feminine care gel of the invention has advantages below：

Finally illustrate, above example is only used for illustrating technical scheme and unrestricted, although with reference to compared with The present invention is described in detail good embodiment, it will be understood by those within the art that, can be to skill of the invention Art scheme is modified or equivalent substitution, and without departing from the objective and scope of technical solution of the present invention, it all should cover at this Among the right of invention.

Claims

1. a kind of placenta hydrolyzes the preparation method of ultrafiltrate, it is characterised in that comprise the following steps：

Step 1, fresh or freezing placenta is weighed, in rejecting manadesma and big blood vessel under cleaning sterile environment, at 4 DEG C -10 DEG C Rinsed well in sterile frozen water, obtain cleaning placenta；

Step 3, the small molecule to weights such as fresh or freezing placentas crushed in placenta in addition and step 1 obtained by step 2 Group's water, is positioned in sterile stainless steel ware, seals, quick-frozen overnight in -20 DEG C or less of temperature, is subsequently placed in 4 DEG C -10 DEG C In the environment of melt completely, repeatedly it is quick-frozen with to melt operation multiple, obtain hydrolyzate；

Step 4, the hydrolyzate obtained in step 3 is carried out in centrifuge to centrifugation -60 minutes 30 minutes, obtain supernatant；Its In, the centrifugal speed of the centrifuge is at least 3500RPM；

Step 5, the supernatant for obtaining step 4 obtain coarse filtration liquid after qualitative filter paper suction filtration, by coarse filtration liquid from 10KD ultrafiltration posts After middle ultrafiltration, abandon and penetrate liquid and stay omission timber, obtain the omission timber that molecular weight is less than 10KD；By the omission timber from 1KD ultrafiltration posts After middle ultrafiltration, abandon omission timber and stay and penetrate liquid, obtain ultrafiltrate of the molecular weight in 1KD-10KD.

2. placenta as claimed in claim 1 hydrolyzes the preparation method of ultrafiltrate, it is characterised in that the freezing in the step 1 Placenta needs to thaw completely in the environment of 4 DEG C -10 DEG C in advance.

3. a kind of placenta hydrolyzes ultrafiltrate, it is characterised in that utilize the placenta hydrolysis ultrafiltrate described in claim any one of 1-2 Preparation method prepare.

4. a kind of placenta as claimed in claim 3 hydrolyzes the application of ultrafiltrate, it is characterised in that the placenta hydrolyzes ultrafiltrate Application in skin care item, female genital tract partial decontamination product or women ovary care cosmetic product is prepared.

5. a kind of feminine care gel, it is characterised in that its component include placenta hydrolysis ultrafiltrate, small-micelle water, NMF, Skin conditioning agent, thickener, pH adjusting agent and freshener, the placenta hydrolysis ultrafiltrate are the placenta described in claim 3 Hydrolyze ultrafiltrate.

6. feminine care gel as claimed in claim 5, it is characterised in that according to each component in the feminine care gel Weight/mass percentage composition meter, including：

The placenta hydrolyzes ultrafiltrate, and content is 30%~70%；

The small-micelle water, content is 10%~50%；

The NMF, content is 8%~20%；

The skin conditioning agent, content is 6%~9%；

The thickener, content is 1%~3%；

The pH adjusting agent, content is 0.05%~0.15%；

The freshener, content is 0.02%~0.2%.

7. feminine care gel as claimed in claim 5, it is characterised in that the NMF is glycerine, propane diols or fourth One or more kinds of mixtures in glycol.

8. feminine care gel as claimed in claim 5, it is characterised in that the skin conditioning agent is glycerine polymethyl Acid esters, ethanol, ammonium hydroxide, n- butanol, isopropanol, edelweiss floral leaf extract, kuh-seng root extract, cordate houttuynia are extracted One or more kinds of mixtures in thing, hydrolytic collagen or hyaluronic acid.

9. feminine care gel as claimed in claim 5, it is characterised in that the thickener is hydroxyethyl cellulose or poly- The mixture of one or two kinds of in sodium acrylate graft starch.

10. a kind of preparation method of feminine care gel as described in claim any one of 5-9, it is characterised in that including with Lower step：

Step 1, placenta is hydrolyzed into ultrafiltrate, skin conditioning agent, freshener be added to successively in small-micelle water, to completely molten Solution, it is well mixed to obtain the first mixture；

Step 2, NMF, thickener be added sequentially in the first mixture in step 1, be completely dissolved it, mixing is equal It is even to obtain the second mixture；