CN107183443A - Amur grape beverage and preparation method and application - Google Patents
Amur grape beverage and preparation method and application Download PDFInfo
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- CN107183443A CN107183443A CN201710329734.8A CN201710329734A CN107183443A CN 107183443 A CN107183443 A CN 107183443A CN 201710329734 A CN201710329734 A CN 201710329734A CN 107183443 A CN107183443 A CN 107183443A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
- A23L2/04—Extraction of juices
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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Abstract
The present invention relates to a kind of amur grape beverage and preparation method and application, raw material components by weight, including：Amur grape, sugar and water.Preparation method：Harvesting no disease and pests harm, the amur grape having no mechanical damage, are cleaned using cleaning agent；Grape is smashed to pieces, isolated pulp, Grape Skin and treaster；The active ingredient in Grape Skin and treaster is extracted respectively, is afterwards mixed pulp, skin, slag extract, freeze-drying, then is mixed with other raw material components, obtains amur grape beverage.The amur grape beverage that the present invention is provided as a pure green beverage, good mouthfeel, it is nutritious, quench the thirst and health properties with excellent relieving summer-heat；It is prepared from from amur grape, without any artificial component, it is to avoid the addition of various additives in traditional beverages, safe without toxic side effect, is had great application prospect；In addition, the amur grape beverage of the present invention has effects that excellent enhancing immunity of organisms, prevention and cure of cardiovascular disease.
Description
Technical field
The present invention relates to technical field of beverage, and in particular to a kind of amur grape beverage and preparation method and application.
Background technology
With the development of society, the raising of living standards of the people, people the demand of food has been not limited to be only
The demand of full abdomen is met, but pursues nutrition, health, the food of safety, people constantly pass through diet while health is focused on
Drink carrys out health care oneself；Especially people are continuously increased to the demand of fruit beverage in recent years, to special fruity, can solve
Heat quenches the thirst and the drink demand with excellent health-care effect is obviously improved.
It is well known that grape is rich in a variety of nutriments, consumer's favorable comment is enjoyed；Volume tartaric acid in grape helps to disappear
Change, it is appropriate to eat a little grapes more, can strengthening the spleen and stomach.Containing mineral calcium, potassium, phosphorus, iron (it is containing compound ferro element in fruit in grape
Most fruit, is the nutraceutical of Anemic patients), and multivitamin B1, B2, B6, C and P etc., also containing a variety of human bodies
Required amino acid, often to eat grape is of great advantage to neurasthenia, excessive fatigue.Grape Skin account for whole fruit weight 8%~
10%, wherein in addition to containing a certain amount of nutrient and abundant aromatic substance, being primarily present in the cyanine in Grape Skin
The materials such as element, tannin, flavonoids, can delay the cellulose in the formation of atherosclerosis, pericarp to be mainly solubility
Food fiber, is easily immediately used by the body；It can not only reduce cholesterol and blood glucose in blood, and to diabetes, constipation, colon
The diseases such as cancer also have certain prevention effect.
However, grape beverage mouthfeel present on current market is single, beneficiating ingredient is lost in seriously and nutrition is unbalanced etc.
Shortcomings；Based on this, after the grape drink for needing a kind of new unique flavor quality better of offer, fully natural green badly.
The content of the invention
For defect of the prior art, the present invention is intended to provide a kind of amur grape beverage and preparation method thereof is with answering
With.The amur grape beverage mouthfeel that the present invention is provided is good, nutritious and quenches the thirst and health properties with excellent relieving summer-heat；Its
It is prepared from from amur grape, prescription proportioning science, without any artificial synthesis group point, it is to avoid prepared by traditional beverages
The addition of various additives in journey, safe without toxic side effect, production cost are low, have great application prospect；In addition, of the invention
The amur grape beverage prepared has effects that excellent enhancing immunity of organisms, suppresses and alleviate angiocardiopathy.
Therefore, the present invention provides following technical scheme：
In a first aspect, the present invention provides a kind of amur grape beverage, raw material components by weight, including：Amur grape 1
~60 parts by weight, sugared 0.5~5 parts by weight and the parts by weight of water 25~99.It is well known that amur grape also known as V. amurensis, are Portugals
Grape section fallen leaves liana, is that perennial liana climbs fruit tree, with the characteristic such as ecosystem, pure natural, pollution-free, is described as the Nature
The soul of remnants is precious；The dark purple black of amur grape fruit, color depth succulence, sweet acid, sugar is relatively low, and berry is nutritious, be it is a kind of not
Reproducible rare pollution-free industry resource, with huge economic development value and broad mass market development prospect.Institute of the present invention
Sugar can be from white granulated sugar and/or xylitol etc..
In the further embodiment of the present invention, including：The parts by weight of amur grape 4~50, sugared 1~3 parts by weight and
The parts by weight of water 25~90.
Second aspect, the present invention provides a kind of preparation method of amur grape beverage, comprises the following steps：S101：Harvesting
Maturity is 80~90%, no disease and pests harm, the amur grape having no mechanical damage, and is cleaned afterwards using cleaning agent；S102：Will
Amur grape after S101 cleanings is smashed to pieces, isolated amur grape pulp, amur grape skin and amur grape slag；S103：Will
Amur grape skin is soaked in the first mixture, is filtered afterwards and is collected filtrate, obtains amur grape bark extract；By wild Portugal
Supercritical CO is used after grape ground-slag is broken2Fluid is extracted, and is filtered afterwards and is collected filtrate, obtains amur grape slag extract；
S104：Amur grape pulp, amur grape bark extract are well mixed with amur grape slag extract, are freeze-dried, afterwards
It is well mixed with other raw material components, obtains amur grape beverage.
The present invention further embodiment in, in S103, the raw material components of the first mixture by weight, including
The parts by weight of Morinda 5~8, the parts by weight of rhizoma alismatis 1~3, the parts by weight of potassium dihydrogen phosphate 2~5 and the parts by weight of water 30~50；First mixes
The preparation method of compound：Will each raw material components crush after be well mixed, boil 50min~90min in 50 DEG C~60 DEG C, obtain the
One mixture.
In the further embodiment of the present invention, in S103, the raw material components of the first mixture also include：Sweet basil 1~2
Parts by weight and the parts by weight of peppermint 2~5.
In the further embodiment of the present invention, in S103, supercritical CO2Fluid extraction is specially：Pressure is
23.90MPa~26.30MPa, temperature be 38 DEG C~45 DEG C, CO2Fluid flow is 12mL/min~20mL/min, extraction time
For 90min~200min.
In the further embodiment of the present invention, in S101, the raw material components of cleaning agent by weight, including tea soap
Plain 0.2~0.8 parts by weight, the parts by weight of sodium chloride 5~8, the parts by weight of citric acid 1~3 and the parts by weight of water 80~120.
In the further embodiment of the present invention, in S104, freeze-drying is specially：Using 0.1~0.2 DEG C/min's
Rate of temperature fall is cooled to -15 DEG C from -8 DEG C, places 10h~20h at -15 DEG C afterwards.
The third aspect, the amur grape beverage that provides of the present invention quenches the thirst, strengthen immunity of organisms, prevents and treats the heart preparing relieving summer-heat
Application in the drink or drink additive of vascular diseases.
The above-mentioned technical proposal that the present invention is provided has advantages below：
(1) applicant has found by numerous studies：The amur grape beverage mouthfeel that the present invention is provided it is good, nutritious and
Quench the thirst with excellent relieving summer-heat and health properties；It is prepared from from amur grape, prescription proportioning science, without anyone
Work synthesizes component, it is to avoid the addition of various additives, safe without toxic side effect, production cost in traditional beverages preparation process
It is low, have great application prospect；In addition, the amur grape beverage that the present invention is prepared has excellent enhancing immunity of organism
Power, suppression and the effect for alleviating angiocardiopathy.
(2) the joyful discovery of applicant：Grape berry, skin and slag are handled respectively using what the present invention was provided, mixed afterwards
Uniform preparation method is closed, can be while active component in retaining amur grape to greatest extent, can be amur grape
In active ingredient fully discharge so that the amur grape beverage finally prepared has excellent effect.
(3) present invention is provided preparation method process stabilizing, yield is high, quality is good, energy consumption is low, raw material are extensive, process
It is simple to save；Production can be completed using existing equipment, equipment requirement is reduced, so as to significantly reduce production cost.
(4) present invention is from amur grape as raw material, and the amur grape beverage prepared is adapted to various crowds and drunk.
Containing 250 multiple nutritional components such as sugar, organic acid, vitamin and inorganic salts in wild V. amurensis, and it is particularly substantial amounts of in grape
The various energy prevention and cure of cardiovascular disease effects such as OPC and Bai Li alcohol, the amur grape beverage that the present invention is prepared is dark red gorgeous
Beautiful, unique flavor, quality better are a very excellent beverages.
The additional aspect and advantage of the present invention will be set forth in part in the description, and will partly become from the following description
Obtain substantially, or recognized by the practice of the present invention.
Embodiment
The embodiment to technical solution of the present invention is described in detail below.Following examples are only used for clearer
Explanation technical scheme, therefore be only used as example, and can not be limited the scope of the invention with this.
Experimental method in following embodiments, is conventional method unless otherwise specified.
Test material used, is to be commercially available from conventional reagent shop unless otherwise specified in following embodiments.
Quantitative test in following examples, is respectively provided with three repetition experiments, and data are the average value of three repetition experiments
Or mean+SD.
Morinda, rhizoma alismatis, sweet basil and peppermint used meet Chinese Pharmacopoeia (version in 2015) one in following embodiments
Pertinent regulations under each medicinal material of text.Before feeding intake, by identification, ingredients material object is consistent with title, and quality meets standard.
The present invention provides a kind of amur grape beverage, raw material components by weight, including：The weight of amur grape 1~60
Part, sugared 0.5~5 parts by weight and the parts by weight of water 25~99.
In addition, the present invention specially devises the method for preparing amur grape beverage, comprise the following steps：
S101：Harvest maturity is 80~90%, no disease and pests harm, the amur grape having no mechanical damage, afterwards using cleaning
Agent is cleaned.Wherein, the raw material components of cleaning agent by weight, including the parts by weight of Tea Saponin 0.2~0.8, sodium chloride 5~
8 parts by weight, the parts by weight of citric acid 1~3 and the parts by weight of water 80~120.
S102：Amur grape after S101 is cleaned is smashed to pieces, isolated amur grape pulp, amur grape skin and wild
Treaster.
S103：Amur grape skin is soaked in the first mixture, filters afterwards and collects filtrate, obtain amur grape skin
Extract；By amur grape ground-slag it is broken after use supercritical CO2Fluid is extracted, and is filtered afterwards and is collected filtrate, obtains open country
Raw treaster extract.Wherein, supercritical CO2Fluid extraction is specially：Pressure is 23.90MPa~26.30MPa, and temperature is 38
DEG C~45 DEG C, CO2Fluid flow is 12mL/min~20mL/min, and extraction time is 90min~200min；First mixture
Raw material components by weight, including the parts by weight of Morinda 5~8, the parts by weight of rhizoma alismatis 1~3, the parts by weight of potassium dihydrogen phosphate 2~5 with
And the parts by weight of water 30~50；And first the raw material components of mixture preferably also include：The parts by weight of sweet basil 1~2 and peppermint 2~5
Parts by weight.
S104：Amur grape pulp, amur grape bark extract are well mixed with amur grape slag extract, freezing is dry
It is dry, it is well mixed afterwards with other raw material components, obtains amur grape beverage.Wherein, freeze-drying is specially：Using 0.1~
0.2 DEG C/min rate of temperature fall is cooled to -15 DEG C from -8 DEG C, places 10h~20h at -15 DEG C afterwards.
Illustrated with reference to embodiment：
Embodiment one
The present invention provides a kind of amur grape beverage, raw material components by weight, including：The parts by weight of amur grape 35,
Sugared 3 parts by weight and the parts by weight of water 78.
By above-mentioned raw materials component, the preparation method of the amur grape beverage provided using the present invention prepares amur grape drink
Material：
S101：Harvest maturity is 90%, no disease and pests harm, the amur grape having no mechanical damage, and is entered afterwards using cleaning agent
Row cleaning；Wherein, the raw material components of cleaning agent by weight, including the parts by weight of Tea Saponin 0.2, the parts by weight of sodium chloride 8, lemon
Each raw material components of cleaning agent, are well mixed, obtain cleaning agent by sour 1 parts by weight and the parts by weight of water 120 afterwards.
S102：Amur grape after S101 is cleaned is smashed to pieces, isolated amur grape pulp, amur grape skin and wild
Treaster.
S103：Amur grape skin is soaked in the first mixture, filters afterwards and collects filtrate, obtain amur grape skin
Extract；By amur grape ground-slag it is broken after use supercritical CO2Fluid is 26.30MPa in pressure, and temperature is 38 DEG C, CO2Fluid
Flow filters to extract 90min under conditions of 12mL/min and collects filtrate, obtain amur grape slag extract afterwards；Wherein,
The raw material components of first mixture by weight, including the parts by weight of Morinda 8, the parts by weight of rhizoma alismatis 1, the weight of potassium dihydrogen phosphate 5
Part and the parts by weight of water 30；It is well mixed, is boiled in 50 DEG C after each raw material components in the first mixture are crushed afterwards
90min, obtains the first mixture.
S104：Amur grape pulp, amur grape bark extract are well mixed with amur grape slag extract, freezing is dry
It is dry, it is well mixed afterwards with other raw material components, obtains amur grape beverage；Wherein, freeze-drying is specially：Using 0.2 DEG C/
Min rate of temperature fall is cooled to -15 DEG C from -8 DEG C, places 20h at -15 DEG C afterwards.
Embodiment two
The present invention provides a kind of amur grape beverage, raw material components by weight, including：The parts by weight of amur grape 4, sugar
3 parts by weight and the parts by weight of water 25.
By above-mentioned raw materials component, the preparation method of the amur grape beverage provided using the present invention prepares amur grape drink
Material：
S101：Harvest maturity is 80%, no disease and pests harm, the amur grape having no mechanical damage, and is entered afterwards using cleaning agent
Row cleaning；Wherein, the raw material components of cleaning agent by weight, including the parts by weight of Tea Saponin 0.8, the parts by weight of sodium chloride 5, lemon
Each raw material components of cleaning agent, are well mixed, obtain cleaning agent by sour 3 parts by weight and the parts by weight of water 80 afterwards.
S102：Amur grape after S101 is cleaned is smashed to pieces, isolated amur grape pulp, amur grape skin and wild
Treaster.
S103：Amur grape skin is soaked in the first mixture, filters afterwards and collects filtrate, obtain amur grape skin
Extract；By amur grape ground-slag it is broken after use supercritical CO2Fluid is 23.90MPa in pressure, and temperature is 45 DEG C, CO2Fluid
Flow filters to extract 200min under conditions of 20mL/min and collects filtrate, obtain amur grape slag extract afterwards；Its
In, the raw material components of the first mixture by weight, including the parts by weight of Morinda 5, the parts by weight of rhizoma alismatis 3, the weight of potassium dihydrogen phosphate 2
Measure part and the parts by weight of water 50；It is well mixed, is boiled in 60 DEG C after each raw material components in the first mixture are crushed afterwards
50min, obtains the first mixture.
S104：Amur grape pulp, amur grape bark extract are well mixed with amur grape slag extract, freezing is dry
It is dry, it is well mixed afterwards with other raw material components, obtains amur grape beverage；Wherein, freeze-drying is specially：Using 0.1 DEG C/
Min rate of temperature fall is cooled to -15 DEG C from -8 DEG C, places 10h at -15 DEG C afterwards.
Embodiment three
The present invention provides a kind of amur grape beverage, raw material components by weight, including：The parts by weight of amur grape 50,
Sugared 1 parts by weight and the parts by weight of water 90.
By above-mentioned raw materials component, the preparation method of the amur grape beverage provided using the present invention prepares amur grape drink
Material：
S101：Harvest maturity is 80%, no disease and pests harm, the amur grape having no mechanical damage, and is entered afterwards using cleaning agent
Row cleaning；Wherein, the raw material components of cleaning agent by weight, including the parts by weight of Tea Saponin 0.5, the parts by weight of sodium chloride 8, lemon
Each raw material components of cleaning agent, are well mixed, obtain cleaning agent by sour 2 parts by weight and the parts by weight of water 100 afterwards.
S102：Amur grape after S101 is cleaned is smashed to pieces, isolated amur grape pulp, amur grape skin and wild
Treaster.
S103：Amur grape skin is soaked in the first mixture, filters afterwards and collects filtrate, obtain amur grape skin
Extract；By amur grape ground-slag it is broken after use supercritical CO2Fluid is 25.30MPa in pressure, and temperature is 40 DEG C, CO2Fluid
Flow filters to extract 150min under conditions of 17mL/min and collects filtrate, obtain amur grape slag extract afterwards；Its
In, the raw material components of the first mixture by weight, including the parts by weight of Morinda 6, the parts by weight of rhizoma alismatis 2, the weight of potassium dihydrogen phosphate 3
Measure part and the parts by weight of water 40；It is well mixed, is boiled in 55 DEG C after each raw material components in the first mixture are crushed afterwards
70min, obtains the first mixture.
S104：Amur grape pulp, amur grape bark extract are well mixed with amur grape slag extract, freezing is dry
It is dry, it is well mixed afterwards with other raw material components, obtains amur grape beverage；Wherein, freeze-drying is specially：Using 0.1 DEG C/
Min rate of temperature fall is cooled to -15 DEG C from -8 DEG C, places 10h at -15 DEG C afterwards.
Example IV
The present invention provides a kind of amur grape beverage, raw material components by weight, including：The parts by weight of amur grape 35,
Sugared 3 parts by weight and the parts by weight of water 78.
By above-mentioned raw materials component, the preparation method of the amur grape beverage provided using the present invention prepares amur grape drink
Material：
S101：Harvest maturity is 90%, no disease and pests harm, the amur grape having no mechanical damage, and is entered afterwards using cleaning agent
Row cleaning；Wherein, the raw material components of cleaning agent by weight, including the parts by weight of Tea Saponin 0.2, the parts by weight of sodium chloride 8, lemon
Each raw material components of cleaning agent, are well mixed, obtain cleaning agent by sour 1 parts by weight and the parts by weight of water 120 afterwards.
S102：Amur grape after S101 is cleaned is smashed to pieces, isolated amur grape pulp, amur grape skin and wild
Treaster.
S103：Amur grape skin is soaked in the first mixture, filters afterwards and collects filtrate, obtain amur grape skin
Extract；By amur grape ground-slag it is broken after use supercritical CO2Fluid is 26.30MPa in pressure, and temperature is 38 DEG C, CO2Fluid
Flow filters to extract 90min under conditions of 12mL/min and collects filtrate, obtain amur grape slag extract afterwards；Wherein,
The raw material components of first mixture by weight, including the parts by weight of Morinda 8, the parts by weight of rhizoma alismatis 1, the weight of potassium dihydrogen phosphate 5
Part, the parts by weight of sweet basil 1 and the parts by weight of peppermint 5 and the parts by weight of water 30；Afterwards by each raw material components powder in the first mixture
It is well mixed after broken, 90min is boiled in 50 DEG C, the first mixture is obtained.
S104：Amur grape pulp, amur grape bark extract are well mixed with amur grape slag extract, freezing is dry
It is dry, it is well mixed afterwards with other raw material components, obtains amur grape beverage；Wherein, freeze-drying is specially：Using 0.2 DEG C/
Min rate of temperature fall is cooled to -15 DEG C from -8 DEG C, places 20h at -15 DEG C afterwards.
Embodiment five
The present invention provides a kind of amur grape beverage, raw material components by weight, including：The parts by weight of amur grape 4, sugar
3 parts by weight and the parts by weight of water 25.
By above-mentioned raw materials component, the preparation method of the amur grape beverage provided using the present invention prepares amur grape drink
Material：
S101：Harvest maturity is 80%, no disease and pests harm, the amur grape having no mechanical damage, and is entered afterwards using cleaning agent
Row cleaning；Wherein, the raw material components of cleaning agent by weight, including the parts by weight of Tea Saponin 0.8, the parts by weight of sodium chloride 5, lemon
Each raw material components of cleaning agent, are well mixed, obtain cleaning agent by sour 3 parts by weight and the parts by weight of water 80 afterwards.
S102：Amur grape after S101 is cleaned is smashed to pieces, isolated amur grape pulp, amur grape skin and wild
Treaster.
S103：Amur grape skin is soaked in the first mixture, filters afterwards and collects filtrate, obtain amur grape skin
Extract；By amur grape ground-slag it is broken after use supercritical CO2Fluid is 23.90MPa in pressure, and temperature is 45 DEG C, CO2Fluid
Flow filters to extract 200min under conditions of 20mL/min and collects filtrate, obtain amur grape slag extract afterwards；Its
In, the raw material components of the first mixture by weight, including the parts by weight of Morinda 5, the parts by weight of rhizoma alismatis 3, the weight of potassium dihydrogen phosphate 2
Measure part, the parts by weight of sweet basil 2 and the parts by weight of peppermint 2 and the parts by weight of water 50；Afterwards by each raw material components in the first mixture
It is well mixed after crushing, boils 50min in 60 DEG C, obtain the first mixture.
S104：Amur grape pulp, amur grape bark extract are well mixed with amur grape slag extract, freezing is dry
It is dry, it is well mixed afterwards with other raw material components, obtains amur grape beverage；Wherein, freeze-drying is specially：Using 0.1 DEG C/
Min rate of temperature fall is cooled to -15 DEG C from -8 DEG C, places 10h at -15 DEG C afterwards.
Embodiment six
The present invention provides a kind of amur grape beverage, raw material components by weight, including：The parts by weight of amur grape 35,
Sugared 3 parts by weight and the parts by weight of water 78.
By above-mentioned raw materials component, the preparation method of the amur grape beverage provided using the present invention prepares amur grape drink
Material：
S101：Harvest maturity is 90%, no disease and pests harm, the amur grape having no mechanical damage, and is carried out afterwards using clear water
Cleaning.
S102：Amur grape after S101 is cleaned is smashed to pieces, isolated amur grape pulp, amur grape skin and wild
Treaster.
S103：Amur grape skin is soaked in the first mixture, filters afterwards and collects filtrate, obtain amur grape skin
Extract；By amur grape ground-slag it is broken after use supercritical CO2Fluid is 26.30MPa in pressure, and temperature is 38 DEG C, CO2Fluid
Flow filters to extract 90min under conditions of 12mL/min and collects filtrate, obtain amur grape slag extract afterwards；Wherein,
The raw material components of first mixture by weight, including the parts by weight of Morinda 8, the parts by weight of rhizoma alismatis 1, the weight of potassium dihydrogen phosphate 5
Part and the parts by weight of water 30；It is well mixed, is boiled in 50 DEG C after each raw material components in the first mixture are crushed afterwards
90min, obtains the first mixture.
S104：Amur grape pulp, amur grape bark extract are well mixed with amur grape slag extract, freezing is dry
It is dry, it is well mixed afterwards with other raw material components, obtains amur grape beverage；Wherein, freeze-drying is specially：Using 0.2 DEG C/
Min rate of temperature fall is cooled to -15 DEG C from -8 DEG C, places 20h at -15 DEG C afterwards.
In addition, the amur grape beverage that various embodiments of the present invention are obtained, by function assessment experiment come its work(of system evaluation
Effect：
First, toxicity test
The small white mouse 60 (each 30 of male and female) for selecting body weight to be 18~22g, is divided into 6 groups, each 5 of every group of male and female are filled respectively
The amur grape beverage that the stomach embodiment of the present invention one to embodiment six is prepared, dosage is 5mL/kg, successive administration 7
My god.When during administration and 1 week after being discontinued, observation small white mouse growth conditions and activity and diet, identify hematology, blood biochemical analysis,
Organ tissue structure and routine urinalysis etc..As a result the health existence of all small white mouses is found, it is without any side effects；Anatomic observation
Blood picture, liver function, each organs and tissues state, are compared indifference with normal small white mouse.It is demonstrated experimentally that the wild Portugal that the present invention is provided
Grape beverage is nontoxic, safe and reliable when eating.
2nd, the physical and chemical index of each embodiment amur grape beverage is determined
According to GB 7101-2015《National food safety standard -- beverage》The amur grape prepared to each embodiment
Beverage is measured, and specific data are as shown in table 1；In addition, the sugariness and heat of each embodiment amur grape beverage are determined, its
In, sugariness is measured using saccharometer, using sucrose as primary standard substance, using sugariness of 10% aqueous sucrose solution at 20 DEG C as 100,
The sugariness of amur grape beverage is obtained in comparison, and measurement result is shown in Table 1.
The physical and chemical index data list of each embodiment amur grape beverage of table 1
| Zinc, copper, iron summation/(mg/L) | Sugariness | Heat (card) | |
| Embodiment one | 18 | 113 | 35 |
| Embodiment two | 17 | 118 | 40 |
| Embodiment three | 18 | 109 | 38 |
| Example IV | 20 | 125 | 26 |
| Embodiment five | 19 | 122 | 29 |
| Embodiment six | 15 | 95 | 123 |
3rd, each embodiment amur grape beverage trial effect is evaluated
The call-up age volunteer of 18~65 years old 100, tastes the amur grape drink that various embodiments of the present invention are prepared
Material, each 100mL evaluates its mouthfeel and trial effect afterwards.
According to the effect of following Standard Judgement amur grape beverage：
It is in good taste：Fragrance is pleasant and sugariness is suitable, is adapted to the taste of vast majority of people, 5 points；It is good in taste：Fragrance is excessively
Strong or lighter, sugariness slightly sweet tea or slightly weaker is adapted to the taste of most people, 4 points；Mouthfeel is bad：Fragrance overrich is excessively light, sweet tea
Spend dense or excessively light, the taste of unsuitable most people.Specific evaluation result is as described in Table 2.
Each embodiment amur grape beverage trial effect evaluation table of table 2
| Mouthfeel/point | Drink rear effect | |
| Embodiment one | 4.55 | Relieving summer-heat quenches the thirst, spirit and QI cleaning, energetic |
| Embodiment two | 4.43 | Relieving summer-heat quenches the thirst, spirit and QI cleaning, energetic |
| Embodiment three | 4.49 | Relieving summer-heat quenches the thirst, spirit and QI cleaning, energetic |
| Example IV | 4.95 | Relieving summer-heat quenches the thirst, spirit and QI cleaning, energetic |
| Embodiment five | 4.92 | Relieving summer-heat quenches the thirst, spirit and QI cleaning, energetic |
| Embodiment six | 3.61 | Relieving summer-heat quenches the thirst |
4th, the effect of each embodiment amur grape beverage in terms of prevention and cure of cardiovascular disease
The wistar strains male rat 80 for selecting body weight to be 180~200g, is divided into 8 groups, every group 10, by each rat fiber crops
It is liquor-saturated to open chest, breathing is maintained, around arteria coroaria sinistra between left auricle of heart and pulmonary conus, and is ligatured.Wherein, first group is
Sham-operation group, using physiological saline gavage, 1mL/ days, totally 4 days；2nd group is myocardial ischemia-reperfusion group, and gavage method is same to do evil through another person
Art group；3rd~8 group is experimental group, amur grape beverage of the embodiment one into embodiment six is gavaged respectively, with 1mL/ days grapes
Beverage gavage.Sham-operation group is not ligatured；The other groups of Reperfu- sions after ligaturing 1 hour.Left coronary artery is ligatured again before animal is put to death,
Take out, rinsed with PBS after her Wen of intra-ventricle injection 1% is blue, freezed 1 hour.Remove after excess tissue, contaminated in 1%TTC
Color 30 minutes (37 DEG C).With weight method calculating myocardium ischemic hazardous area (her Wen Lan Weiran areas), infarcted region (TTC Wei Ran areas).
The result of variations for observing each rat myocardial infarct size is found：Sham-operation has no myocardial infarction phenomenon in 7 hours；Cardiac muscle
1 hour Reperfu- sion of ischemic after 6 hours myocardial infarction it is obvious；It is big that the amur grape beverage of the present invention can be obviously reduced myocardial reperfusion
The myocardial infarct size of mouse, shows that the amur grape beverage of the present invention becomes to ischemic myocardial cells Reperfu- sion myocardium cell necrosis
Change has good protective effect, specific as shown in table 3：
The effect of each embodiment amur grape beverage of table 3 in terms of prevention and cure of cardiovascular disease
Certainly, except the situation that embodiment one is enumerated to embodiment six, the species and percentage by weight of other raw material components,
Preparation condition etc. is also possible.
The amur grape beverage mouthfeel that the present invention is provided is good, nutritious and quenches the thirst and health with excellent relieving summer-heat
Energy；It is prepared from from amur grape, prescription proportioning science, without any artificial synthesis group point, it is to avoid traditional beverages
The addition of various additives in preparation process, safe without toxic side effect, production cost are low, have great application prospect；In addition,
The amur grape beverage that the present invention is prepared has excellent enhancing immunity of organisms, suppresses and alleviate the work(of angiocardiopathy
Effect.In the description of the invention, it is to be understood that term " first ", " second " be only used for describe purpose, and it is not intended that
Indicate or imply relative importance or the implicit quantity for indicating indicated technical characteristic.Thus, " first ", " are defined
~individual or more this feature can be expressed or be implicitly included to two " feature.In the description of the invention, " multiple "
Two or more is meant that, unless otherwise specifically defined.
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show
The description of example " or " some examples " etc. means to combine specific features, structure, material or the spy that the embodiment or example are described
Point is contained at least one embodiment of the present invention or example.In this manual, to the schematic representation of above-mentioned term not
Identical embodiment or example must be directed to.Moreover, specific features, structure, material or the feature of description can be with office
Combined in an appropriate manner in one or more embodiments or example.In addition, in the case of not conflicting, the skill of this area
Art personnel can be tied the not be the same as Example or the feature of example and non-be the same as Example or example described in this specification
Close and combine.
Although embodiments of the invention have been shown and described above, it is to be understood that above-described embodiment is example
Property, it is impossible to limitation of the present invention is interpreted as, one of ordinary skill in the art within the scope of the invention can be to above-mentioned
Embodiment is changed, changed, replacing and modification.
Claims (9)
1. a kind of amur grape beverage, it is characterised in that raw material components by weight, including：
The parts by weight of amur grape 1~60, sugared 0.5~5 parts by weight and the parts by weight of water 25~99.
2. amur grape beverage according to claim 1, it is characterised in that：
The parts by weight of amur grape 4~50, sugared 1~3 parts by weight and the parts by weight of water 25~90.
3. the preparation method of amur grape beverage according to claim 1 or claim 2, it is characterised in that comprise the following steps：
S101：Harvest maturity is 80~90%, no disease and pests harm, the amur grape having no mechanical damage, and is entered afterwards using cleaning agent
Row cleaning；
S102：Amur grape after the S101 is cleaned is smashed to pieces, isolated amur grape pulp, amur grape skin and wild
Treaster；
S103：The amur grape skin is soaked in the first mixture, filters afterwards and collects filtrate, obtain amur grape skin
Extract；By the amur grape ground-slag it is broken after use supercritical CO2Fluid is extracted, and is filtered afterwards and is collected filtrate, obtains
To amur grape slag extract；
S104：The amur grape pulp, amur grape bark extract are well mixed with amur grape slag extract, freezing is dry
It is dry, it is well mixed afterwards with other raw material components, obtains amur grape beverage.
4. the preparation method of amur grape beverage according to claim 3, it is characterised in that：
In the S103, the raw material components of first mixture by weight, including the parts by weight of Morinda 5~8, rhizoma alismatis 1
~3 parts by weight, the parts by weight of potassium dihydrogen phosphate 2~5 and the parts by weight of water 30~50.
5. the preparation method of amur grape beverage according to claim 4, it is characterised in that：
In the S103, the raw material components of first mixture also include：The parts by weight of sweet basil 1~2 and the weight of peppermint 2~5
Part.
6. the preparation method of amur grape beverage according to claim 3, it is characterised in that：
In the S103, the supercritical CO2Fluid extraction is specially：Pressure is 23.90MPa~26.30MPa, and temperature is 38 DEG C
~45 DEG C, CO2Fluid flow is 12mL/min~20mL/min, and extraction time is 90min~200min.
7. the preparation method of amur grape beverage according to claim 3, it is characterised in that：
In the S101, the raw material components of the cleaning agent by weight, including the parts by weight of Tea Saponin 0.2~0.8, sodium chloride
5~8 parts by weight, the parts by weight of citric acid 1~3 and the parts by weight of water 80~120.
8. the preparation method of amur grape beverage according to claim 3, it is characterised in that：
In the S104, the freeze-drying is specially：Using 0.1~0.2 DEG C/min rate of temperature fall -15 are cooled to from -8 DEG C
DEG C, place 10h~20h at -15 DEG C afterwards.
9. any one of the claim 1 or 2 amur grape beverage quenches the thirst, strengthen immunity of organisms, prevents and treats the heart preparing relieving summer-heat
Application in the drink or drink additive of vascular diseases.
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Cited By (1)
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| CN115721023A (en) * | 2022-11-29 | 2023-03-03 | 民强(昆山)食品科技有限公司 | Dietary fiber powder solid beverage |
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