CN107155985B - Breeding method for resisting high ammonia nitrogen and growth traits of marsupenaeus japonicus breeding population - Google Patents
Breeding method for resisting high ammonia nitrogen and growth traits of marsupenaeus japonicus breeding population Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
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Abstract
一种日本囊对虾育种群体抗高氨氮与生长性状的选育方法，包括如下步骤：(1)亲虾引进、(2)家系建立、(3)抗高氨氮预实验、(4)选育家系的抗高氨氮实验、(5)抗高氨氮数据记录、(6)遗传评估模型建立、(7)家系留种、(8)第二代家系建立。本发明可选育出一种抗高氨氮与生长优势的日本囊对虾新品种，增强日本囊对虾对氨氮浓度的抗逆性，提升日本囊对虾的成活率和生长速度。A breeding method for resistance to high ammonia nitrogen and growth traits in a breeding population of Penaeus japonicus, comprising the following steps: (1) introduction of broodstock, (2) establishment of a family, (3) pre-experiment of resistance to high ammonia nitrogen, and (4) breeding of a family Anti-high ammonia nitrogen experiment, (5) anti-high ammonia nitrogen data record, (6) establishment of genetic evaluation model, (7) family breeding, (8) second generation family establishment. The invention can breed a new variety of Penaeus japonicus with high ammonia nitrogen resistance and growth advantage, enhance the stress resistance of the Penaeus japonicus to ammonia nitrogen concentration, and improve the survival rate and growth rate of the Penaeus japonicus.
Description
Technical Field
The invention relates to the field of aquatic product breeding, in particular to a breeding method for resisting high ammonia nitrogen and growth traits of a marsupenaeus japonicus breeding group.
Background
The ammonia nitrogen is a harmful substance commonly existing in aquaculture, is one of main components of excrement of the prawns, is a final product for decomposing nitrogenous organic matters such as residual baits, feces, animal and plant corpses and the like of the prawns, and has the following effects on the physiological function of the penaeus japonicus:
1. it can permeate into the blood of prawns to cause the concentration of hemolymph ammonia nitrogen to rise, the concentration of oxygenated hemocyanin to reduce, the respiratory function of the prawns and the oxygen carrying capacity of the blood to weaken, the tissue hypoxia of the prawns, the death of the cultured prawns in a large amount in severe cases to cause the culture failure, and the popularization of the culture of the penaeus japonicus is not facilitated,
2. the ammonia nitrogen concentration can affect the growth, molting and other physiological function abnormalities of the prawns, so that the oxygen consumption of the prawns is increased, the physiological metabolism of organisms is disordered, and the disease resistance is reduced.
Therefore, the breeding of a new breed of the penaeus japonicus with high tolerance to ammonia nitrogen concentration and growth advantage has very important significance, can improve the breeding success rate of the penaeus japonicus, and is beneficial to industrial development. However, the prior art has no record of a breeding method for resisting high ammonia nitrogen and growth traits of a marsupenaeus japonicus breeding group.
Disclosure of Invention
The invention provides a breeding method for resisting high ammonia nitrogen and growth traits of a marsupenaeus japonicus breeding population, which breeds a new variety of marsupenaeus japonicus with high ammonia nitrogen resistance and growth advantages, enhances the stress resistance of the marsupenaeus japonicus to the ammonia nitrogen concentration, and improves the survival rate and the growth speed of the marsupenaeus japonicus.
A breeding method of Penaeus japonicus breeding population for resisting high ammonia nitrogen and growth traits comprises the following steps:
(1) parent shrimp introduction: collecting the multiple tails of wild groups of the penaeus japonicus, evenly distributing the multiple tails to a plurality of breeding ponds for breeding, and promoting the gonad maturation of the penaeus japonicus by using nutrient substances;
(2) family establishment: removing the unilateral eye handles after the parent shrimps are mature and stable, selecting the parent shrimps with full and continuous gonads and mature development to lay eggs in a spawning barrel every night, putting 1 spawning barrel in 1 parent shrimp, substituting the parent shrimps into 1 full-sib family, putting the parent shrimps which do not lay eggs back to the original breeding pond the next day, and putting the parent shrimps which have laid eggs back to other breeding ponds; after eggs are hatched, collecting nauplii, transferring the nauplii into a breeding barrel of a scientific research workshop after disinfection to start individual breeding of shrimp fries, establishing 100 families according to the process within 10 days, numbering the families in sequence, and eliminating part of families in the breeding process; all families are independently cultivated, and the families are strictly prevented from being mixed with each other;
(3) high ammonia nitrogen resistance preliminary experiment: adopting a sharp toxicity attack experiment, the test solution is NH4Dissolving Cl in culture seawater to prepare the solution, setting high ammonia nitrogen concentration gradients and 1 control group, wherein the high ammonia nitrogen concentration gradients are respectively 4, 8, 16, 32, 64 and 128mg/L, each group is provided with 1 parallel group, counting the number of surviving individuals after 48h of toxicity attack, and calculating the semi-lethal concentration according to a linear regression method;
(4) and (3) performing high ammonia nitrogen resistance experiment of breeding families: the high ammonia nitrogen resistance experiment is carried out in a breeding pond, a plurality of seawater are added, and seawater NH is prepared4The Cl concentration is the calculated semi-lethal concentration, 30 individuals are randomly selected by each family and are put into a screen frame, 3 repeated groups of 1 family are provided, each screen frame is marked and is uniformly placed in a breeding pond according to the serial number, the feeding is not carried out in the experimental process, the number of dead individuals is counted after the inspection is carried out once every a period of time, and the growth characters of the dead individuals are measured;
(5) and (3) recording high ammonia nitrogen resistant data: recording individual phenotype data, arranging according to family sequence, wherein all individual growth traits correspond to stress-resistant traits one by one, setting 1 genetic group for the model, incorporating parents and parents into the genetic group, and sorting and arranging the data according to variance components and requirements of software used for genetic parameter estimation; wherein, the survival character is a classification variable of two-term distribution, the survival state of an individual is represented by binary data, 1 is survival, 0 is death, a model is built according to a generalized linear model, and the survival rate of a family is counted;
(6) establishing a genetic evaluation model: establishing a multi-character linear animal model, and sectioning the observed value y of each individual growth character into:
yijk＝u+Hi+bdj+ak+eijk
wherein i, j, k respectively represent the pool number, the age number, the individual number, yijkIs observed value of the character, u is overall average value, Hi is fixed effect, djIs a age covariate, b is a regression coefficient, akFor individual random effects, eijkIs a random residual effect; the heritability calculation formula of the body length, the abdomen length and the body weight is as follows:
in the formula sigmaa 2Is an additive genetic variance component, σe 2Are random variance components.
After the stress resistance experiment is finished, counting survival conditions of all experimental individuals to obtain survival rates of all families, estimating variance components of survival characters by applying a threshold model and a generalized linear mixed model method, wherein a breeding analysis model is a male and female livestock model:
in the formula, yijklsIs the survival status of the Sth individual, (1 is survival, 0 is death), LijklsAs a latent variable, if Lijkls>0, then yijklsIf y is 1ijkls≦ 0, then yijks0, u is the overall average, Hi is the fixed effect, djIs a age covariate, b is a regression coefficient, skFor the kth paternal additive genetic Effect, dlAdditive genetic effect for the first female parent;
survival trait heritability calculation formula:
in the formula, σsd 2Is the mean value of variance components of male and female animals, sigmae 2For residual variance components, the nonlinear connection function used by the generalized linear mixture model is Logistic, and the Logistic distribution value is
In the iterative REML process, the integral maximization is ensured, and the convergence standard is that the variance of the estimated value obtained by two iterations is less than 10-6(ii) a Estimating genetic correlation and phenotype correlation coefficients of body length, abdomen length and weight by using a software model, estimating genetic correlation and phenotype correlation parameters of growth traits and high ammonia nitrogen resistance traits by using a multi-trait threshold model, wherein the established families are holomorphic families;
(7) family seed reservation: calculating the average stress resistance character and growth character breeding values of the families by using genetic evaluation software, and weighting according to the high ammonia nitrogen resistance character and the growth character 1:1 to obtain the comprehensive breeding values of all families; sorting according to the comprehensive breeding value, reserving 10 families which are ranked at the top, eliminating the rest families, and continuously breeding the reserved families to the specification of parent shrimps;
(8) establishing a second generation family: selecting 20-50 parent shrimps from each family of the reserved family to a breeding pond for temporary breeding to ensure the parent shrimps to naturally mate, marking the eye handle rings of the parent shrimps, attaching different mark numbers to the eye handle rings of different families for identification, avoiding the occurrence of inbreeding, establishing 100 second-generation families according to the method, and breeding the new species with obvious advantages of high ammonia nitrogen resistance and growth according to the standard breeding processes of breeding, high ammonia nitrogen resistance and growth.
Preferably, in the process of introducing parent shrimps, iodine and strong chlorine are used for disinfecting a water body, and live baits are disinfected and fed after virus detection; the used nutrient substances include strong red vitamins, ammonia nitrogen calcium, red worm, green worm, squid, etc.
Preferably, in the family establishment process, the water quality is monitored and improved according to the growth condition of parent shrimps, the culture density is adjusted, the bait feeding times and the variety are adjusted adaptively, when the parent shrimps are cultured to 150 days old, 30 shrimps are selected randomly from each family, and the body length, the abdominal length and the body weight are measured.
Preferably, during the anti-high ammonia nitrogen pre-experiment, a linear regression equation is established by taking the number of dead individuals as independent variables, and the independent variables are set to 1/2 of the total number of experiments, so as to calculate the semi-lethal concentration.
Preferably, the software used in the processes of high ammonia nitrogen resistant data recording, genetic evaluation model establishment and family seed reserving is ASReml software.
The method has the advantages that families need to be established in a large scale, and excellent families with high tolerance are bred through high ammonia nitrogen stress, so that the ammonia nitrogen tolerance and the growth advantage of the marsupenaeus japonicus are improved, the survival rate and the growth speed of the marsupenaeus japonicus are improved, and the method is favorable for breeding and popularization of the marsupenaeus japonicus.
Detailed Description
The present invention will be described in further detail with reference to the following embodiments.
The embodiment of the invention provides a breeding method for resisting high ammonia nitrogen and growth traits of a marsupenaeus japonicus breeding group, which comprises the following steps:
(1) parent shrimp introduction: collecting 1000 tails of wild groups of the marsupenaeus japonicus, evenly distributing the wild groups to four breeding ponds for breeding, wherein the number of the wild groups is 250 tails in each pond, and using nutrient substances to promote the gonad maturation of the shrimps, and the breeding ponds can adopt a specification of 5m multiplied by 1.2 m. In the embodiment, 1000 tails and four rearing ponds are selected, and the shrimp tails and the rearing pond can be determined according to actual conditions in specific application.
(2) Family establishment: removing the unilateral eyestalk after the parent shrimps are mature and stable, selecting the parent shrimps with full and continuous gonads and developing to more than IV period to lay eggs in the egg laying barrel every night, putting 1 egg laying barrel in 1 parent shrimp, substituting 1 full sibling family after birth, putting the parent shrimps which do not lay eggs back to the original breeding pond the next morning, and putting the parent shrimps which have laid eggs back to other breeding ponds; after the eggs are hatched out of the nauplii, collecting the nauplii, transferring the nauplii into a breeding barrel of a scientific research workshop after disinfection to start to breed the shrimp fries independently, establishing 100 families according to the process within 10 days, and numbering the families in sequence, wherein part of the families are eliminated due to death or other reasons in the breeding process; all families are independently cultivated, and the families are strictly prevented from being mixed with each other;
(3) high ammonia nitrogen resistance preliminary experiment: adopting a sharp toxicity attack experiment, the test solution is NH4Cl is dissolved in culture seawater, high ammonia nitrogen concentration gradient and 1 control group are set, the control group uses the same culture seawater without adding NH4Cl; the high ammonia nitrogen concentration gradients are respectively 4, 8, 16, 32, 64 and 128mg/L, 1 parallel group is arranged in each group, the number of surviving individuals after 48 hours of toxicity attack is counted, the semi-lethal concentration is calculated according to a linear regression method, specifically, a linear regression equation is established by taking the number of dead individuals as an independent variable, and the independent variable is set as 1/2 of the total number of experiments, so that the semi-lethal concentration is calculated. The specific content of the linear regression equation can be referred to the current existing death number statistical algorithm.
(4) And (3) performing high ammonia nitrogen resistance experiment of breeding families: the high ammonia nitrogen resistance experiment is carried out in a breeding pond, and seawater NH is allocated4The Cl concentration is the calculated semi-lethal concentration, and the prepared seawater is added into the rearing pond, wherein the adding amount of the seawater is specifically determined according to the scale of the rearing pond, for example, 15 parts of seawater is added into the rearing pond with the size of 5m multiplied by 1.2 m. Randomly selecting 30 individuals from each family, putting the individuals into a net frame, uniformly placing 1 family into a breeding pond by 3 repeated groups, marking each net frame, uniformly placing the net frame into the breeding pond according to the number, not feeding materials in the experimental process, checking once every 8 hours or so, counting the number of dead individuals, and measuring the growth traits of the dead individuals; because the dietary habit of the marsupenaeus japonicus is that the individuals after partial shelling are not completely eaten, the growth character data of all experimental individuals cannot be completely obtained, and sampling data can be selected for the part.
(5) And (3) recording high ammonia nitrogen resistant data: and recording the phenotype data of the individuals, and arranging the phenotype data according to the family sequence, wherein the growth traits of all the individuals correspond to the stress resistance traits one by one. For parent shrimps originally introduced, the method can be used according to the producing area
Setting 1 genetic group for a genetic evaluation model, putting parents and parents into the genetic groups, and sorting and arranging data according to the requirements of software used for estimating variance components and genetic parameters; taking ASReml software as an example, the pedigree relationship and the data should be the same file, and the phenotypic parameters are calculated by the SPSS21.0 software package. Wherein, the survival character is a classification variable of two-term distribution, the survival state of an individual is represented by binary data, 1 is survival, 0 is death, a model is built according to a generalized linear model, and the survival rate of a family is counted. The target distribution of the generalized linear model adopts binomial distribution.
(6) Establishing a genetic evaluation model: establishing a multi-character linear animal model, and sectioning the observed value y of each individual growth character into:
yijk＝u+Hi+bdj+ak+eijk
wherein i, j, k respectively represent the pool number, the age number, the individual number, yijkIs observed value of the character, u is the overall average value, HiFor a fixing effect, djIs a age covariate, b is a regression coefficient, akFor individual random effects, eijkFor the random residual effect, most of the penaeus japonicus individuals are still small, the sex is difficult to distinguish, and the sex does not influence experimental data, so that the sex is not included as a fixed effect;
the heritability calculation formula of the body length, the abdomen length and the body weight of the marsupenaeus japonicus is as follows:
in the formula sigmaa 2Is an additive genetic variance component, σe 2Are random variance components.
After the stress resistance experiment is finished, counting survival conditions of all experimental individuals to obtain survival rates of all families, and estimating variance components of survival characters by using a threshold model and a Generalized Linear Mixed Model (GLMM) method, wherein the threshold model and the generalized linear mixed model can adopt the existing models, the nonlinear connection function used by the generalized linear mixed model is Logistic, and the Logistic distribution value is Logistic
The breeding analysis model is a male and female animal model:
in the formula, yijklsIs the survival status of the Sth individual, (1 is survival, 0 is death), LijklsAs a latent variable, if Lijkls>0, then yijklsIf y is 1ijkls≦ 0, then yijks0, u is the overall average, Hi is the fixed effect, djIs a age covariate, b is a regression coefficient, skFor the kth paternal additive genetic Effect, dlAdditive genetic effect for the first female parent;
survival trait heritability calculation formula:
in the formula, σsd 2Is the mean value of variance components of male and female animals, sigmae 2Is a residual variance component;
in the iterative REML process, the integral maximization is ensured, and the convergence standard is that the variance of the estimated value obtained by two iterations is less than 10-6(ii) a Estimating genetic correlation and phenotype correlation coefficients of body length, abdomen length and body weight by using a software model, such as ASReml software, and estimating genetic correlation and phenotype correlation parameters of growth traits and high ammonia nitrogen resistance traits by using a multi-trait threshold model, wherein the established families are homomorphic families;
(7) family seed reservation: calculating the average stress resistance character and growth character breeding values of the families by using genetic evaluation software, and weighting according to the high ammonia nitrogen resistance character and the growth character 1:1 to obtain the comprehensive breeding values of all families; sorting according to the comprehensive breeding value, reserving 10 families which are ranked at the top, eliminating the rest families, adjusting the breeding density of the reserved families, enhancing nutrition, regulating and controlling water quality and the like, and breeding to the specification of parent shrimps;
(8) establishing a second generation family: selecting 20-50 parent shrimps from each family of the reserved family to a breeding pond for temporary breeding to ensure the parent shrimps to naturally mate, marking the eye handle rings of the parent shrimps, attaching different marks to the eye handle rings of different families so as to be convenient to identify and avoid the occurrence of near-affinity mating, establishing 100 second-generation families according to the method, and breeding the shrimps by using a standard breeding process of breeding, high ammonia nitrogen resistance and growth until breeding a new variety with obvious high ammonia nitrogen resistance and growth advantages, so that the breeding of the Japanese scrotum shrimps is completed.
Wherein, in the process of introducing the parent shrimps, iodine and strong ammonia essence are used for disinfecting the water body, and the parent shrimps are fed after live bait disinfection and virus detection so as to prevent the parent shrimps from being infected by diseases and improve the survival rate of the parent shrimps. The nutrient substances used for promoting gonad maturation include strong red vitamin and/or calcium ammonia nitrogen.
In the process of shrimp cultivation and family establishment, monitoring of water quality, cultivation density, virus detection and the like should be implemented, water quality is improved according to the growth condition of parent shrimps, cultivation density is adjusted, and bait feeding times and types are adaptively adjusted, so that the shrimps have proper growth environment, and the survival rate and growth speed of the shrimps are improved. When the shrimps are cultivated to 150 days old, 30 shrimps can be randomly selected from each family, and the body length, the abdomen length and the body weight are measured to know the growth condition of the shrimps in real time.
In the embodiment, the used software is ASReml software in the processes of high ammonia nitrogen resistant data recording, genetic evaluation model establishment and family seed reserving, and other software can be used according to actual needs. The threshold model, the generalized linear mixed model, the multi-character linear animal model, and the like can all refer to the existing related models, and the specific contents thereof are not described herein.
The foregoing is a more detailed description of the present invention that is presented in conjunction with specific embodiments, and the practice of the invention is not to be considered limited to those descriptions. It will be apparent to those skilled in the art that a number of simple derivations or substitutions can be made without departing from the inventive concept.
Claims (5)
1. A breeding method of Penaeus japonicus breeding population for resisting high ammonia nitrogen and growth traits is characterized by comprising the following steps:
(1) parent shrimp introduction: collecting the multiple tails of wild groups of the penaeus japonicus, evenly distributing the multiple tails to a plurality of breeding ponds for breeding, and promoting the gonad maturation of the penaeus japonicus by using nutrient substances;
(2) family establishment: removing the unilateral eye handles after the parent shrimps are mature and stable, selecting the parent shrimps with full and continuous gonads and mature development to lay eggs in a spawning barrel every night, putting 1 spawning barrel in 1 parent shrimp, substituting the parent shrimps into 1 full-sib family, putting the parent shrimps which do not lay eggs back to the original breeding pond the next day, and putting the parent shrimps which have laid eggs back to other breeding ponds; after eggs are hatched, collecting nauplii, transferring the nauplii into a breeding barrel of a scientific research workshop after disinfection to start individual breeding of shrimp fries, establishing 100 families according to the process within 10 days, numbering the families in sequence, and eliminating part of families in the breeding process; all families are independently cultivated, and the families are strictly prevented from being mixed with each other;
(3) high ammonia nitrogen resistance preliminary experiment: adopting a sharp toxicity attack experiment, the test solution is NH4Dissolving Cl in culture seawater to prepare the solution, setting high ammonia nitrogen concentration gradients and 1 control group, wherein the high ammonia nitrogen concentration gradients are respectively 4, 8, 16, 32, 64 and 128mg/L, each group is provided with 1 parallel group, counting the number of surviving individuals after 48h of toxicity attack, and calculating the semi-lethal concentration according to a linear regression method;
(4) and (3) performing high ammonia nitrogen resistance experiment of breeding families: the high ammonia nitrogen resistance experiment is carried out in a breeding pond, a plurality of seawater are added, and seawater NH is prepared4The Cl concentration is the calculated semi-lethal concentration, 30 individuals are randomly selected by each family and are put into a screen frame, 3 repeated groups of 1 family are provided, each screen frame is marked and is uniformly placed in a breeding pond according to the serial number, the feeding is not carried out in the experimental process, the number of dead individuals is counted after the inspection is carried out once every a period of time, and the growth characters of the dead individuals are measured;
(5) and (3) recording high ammonia nitrogen resistant data: recording individual phenotype data, arranging according to family sequence, wherein all individual growth traits correspond to stress-resistant traits one by one, setting 1 genetic group for the model, incorporating parents and parents into the genetic group, and sorting and arranging the data according to variance components and requirements of software used for genetic parameter estimation; wherein, the survival character is a classification variable of two-term distribution, the survival state of an individual is represented by binary data, 1 is survival, 0 is death, a model is built according to a generalized linear model, and the survival rate of a family is counted;
(6) establishing a genetic evaluation model: establishing a multi-character linear animal model, and sectioning the observed value y of each individual growth character into:
yijk＝u+Hi+bdj+ak+eijk
wherein i, j, k respectively represent the pool number, the age number, the individual number, yijkAs observed for the trait, u is the overall mean, H i is the fixed effect, djIs a age covariate, b is a regression coefficient, akFor individual random effects, eijkIs a random residual effect; the heritability calculation formula of the body length, the abdomen length and the body weight is as follows:
in the formula sigmaa 2Is an additive genetic variance component, σe 2Is a random variance component;
after the stress resistance experiment is finished, counting survival conditions of all experimental individuals to obtain survival rates of all families, estimating variance components of survival characters by applying a threshold model and a generalized linear mixed model method, wherein a breeding analysis model is a male and female livestock model:
in the formula, yijklsSurvival status of the Sth individual, 1 is survival, 0 is death, LijklsAs a latent variable, if Lijkls>0, then yijklsIf y is 1ijkls≦ 0, then yijks0, u is the overall average, Hi is the fixed effect, djIs a age covariate, b is a regression coefficient, skFor the kth paternal additive genetic Effect, dlAdditive genetic effect for the first female parent;
survival trait heritability calculation formula:
in the formula, σsd 2Is the mean value of variance components of male and female animals, sigmae 2For residual variance components, the nonlinear connection function used by the generalized linear mixture model is Logistic, and the Logistic distribution value is
In the iterative REML process, the integral maximization is ensured, and the convergence standard is that the variance of the estimated value obtained by two iterations is less than 10-6(ii) a Estimating genetic correlation and phenotype correlation coefficients of body length, abdomen length and weight by using a software model, and estimating genetic correlation and phenotype correlation of growth traits and high ammonia nitrogen resistance traits by using a multi-trait threshold modelParameters, all established families are full sibling families;
(7) family seed reservation: calculating the average stress resistance character and growth character breeding values of the families by using genetic evaluation software, and weighting according to the high ammonia nitrogen resistance character and the growth character 1:1 to obtain the comprehensive breeding values of all families; sorting according to the comprehensive breeding value, reserving 10 families which are ranked at the top, eliminating the rest families, and continuously breeding the reserved families to the specification of parent shrimps;
(8) establishing a second generation family: selecting 20-50 parent shrimps from each family of the reserved family to a breeding pond for temporary breeding to ensure the parent shrimps to naturally mate, marking the eye handle rings of the parent shrimps, attaching different marks to the eye handle rings of different families for identification, avoiding the occurrence of inbreeding, establishing 100 second-generation families according to the method, and breeding the new species with obvious advantages of high ammonia nitrogen resistance and growth resistance according to the breeding process of standardized breeding, breeding and high ammonia nitrogen resistance and growth resistance.
2. The method of claim 1, wherein:
in the process of introducing parent shrimps, disinfecting a water body by using iodine and strong ammonia essence, and feeding after disinfecting baits; the nutrient substances include red vitamin and/or calcium ammonia nitrogen.
3. The method of claim 1, wherein:
in the family establishment process, water quality is monitored and improved, breeding density is adjusted, bait feeding times and type adaptation are adjusted according to the growth condition of parent shrimps, when the parent shrimps are bred to 150 days old, 30 shrimps are randomly selected from each family, and body length, abdomen length and body weight are measured.
4. The method of claim 1, wherein:
in the process of the anti-high ammonia nitrogen pre-experiment, a linear regression equation is established by taking the number of dead individuals as independent variables, and the independent variables are set to 1/2 of the total number of experiments, so that the semi-lethal concentration is calculated.
5. The method of claim 1, wherein:
in the processes of high ammonia nitrogen resistant data recording, genetic evaluation model establishment and family seed reserving, the used software is ASReml software.
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