CN107144690B - A kind of analyzer magnetic cleaning separator of magnet unilateral side arrangement - Google Patents
A kind of analyzer magnetic cleaning separator of magnet unilateral side arrangement Download PDFInfo
- Publication number
- CN107144690B CN107144690B CN201710506691.6A CN201710506691A CN107144690B CN 107144690 B CN107144690 B CN 107144690B CN 201710506691 A CN201710506691 A CN 201710506691A CN 107144690 B CN107144690 B CN 107144690B
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- magnets
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- cleaning
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/10—Cleaning involving contact with liquid with additional treatment of the liquid or of the object being cleaned, e.g. by heat, by electricity or by vibration
- B08B3/102—Cleaning involving contact with liquid with additional treatment of the liquid or of the object being cleaned, e.g. by heat, by electricity or by vibration with means for agitating the liquid
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
- G01N21/763—Bioluminescence
Abstract
The present invention relates to a kind of analyzer magnetic cleaning separators of magnet unilateral side arrangement, it include: the reaction cup transport assembly for reaction cup to be carried out to horizontal transport, magnetic cleaning separating mechanism, for the cleaning needle assemblies of cleaning solution to be drawn and injected from reaction cup, the magnetic cleaning separating mechanism includes the first group of magnets set gradually, second group of magnets and third group of magnets, three group of magnets are provided with reasonable magnet arrangements and magnetic pole arrangement, magnetic bead is set to be adsorbed on reaction cup wall of cup or rotate in reaction cup, so that the unbonded object for making to be wrapped in when cleaning magnetic bead in conjugate releases, preferably retain that a part of reactant in conjunction with magnetic bead, and it ensure that the time during entire Magneto separate can be short as far as possible, it can also finally guarantee that the magnetic bead after reaction cup enters measuring chamber in cup can sufficiently be reacted with substrate, and Save substrate solution.
Description
Technical field
The present invention relates to a kind of analyzer magnetic cleaning separators of magnet unilateral side arrangement, belong to medical device technology neck
Domain.
Background technique
Chemiluminescence immunoassay detection becomes the most important means of ion vitro immunization detection increasingly, and wherein immune complex has
Effect separation is the key link of chemiluminescence immunoassay detection, and separating effect directly affects immune detection result.Automatically it is being immunized
In detection system, currently used immune complex separation method mainly has centrifugal separation, web partition method and Magneto separate
Method.The major defect of centrifugal separation is to easily cause that compound loses or impurity residual, the shortcomings that web partition method are mainly
Mesh local stoppages are easily caused when complex concentration is bigger than normal, influence passing through for impurity, and magnetic separation technique is realizing magnetic
Start to get a lot of applications in chemiluminescence immunoassay detecting instrument after the antibody coating of grain.
Magnetic separation method in biology has easy magnetization ability and specific binding capacity simultaneously using immunomagnetic beads, enables it
It is specifically bound with target substance, then is retained the reactant in conjunction with magnetic bead by external magnetic field, it is extra to separate
Reactant.Magnetic separation method is higher than centrifugal separation and web partition method, and entire Magneto separate process in terms of separation purity
In time can be very short, but the washing effect of existing magnetic separation method is not good enough.
Summary of the invention
The present invention provides one to solve the existing magnetic cleaning separator technical problem not good enough to the washing effect of magnetic bead
The analyzer magnetic cleaning separator that kind there is the magnet unilateral side of good washing effect to arrange magnetic bead.
The present invention is that technical solution used by solving its technical problem is:
The present invention provides a kind of analyzer magnetic cleaning separator of magnet unilateral side arrangement, comprising: for by reaction cup into
The reaction cup transport assembly of row horizontal transport, magnetic cleaning separating mechanism, for the cleaning of cleaning solution to be drawn and injected from reaction cup
Needle assemblies, the magnetic cleaning separating mechanism include:
Several group of magnets are located at reaction cup conveying assembly transmission belt the same side and along reaction cup conveying assembly transmission direction
Arrangement；
Group of magnets includes the first group of magnets, the second group of magnets and third group of magnets set gradually；First group of magnets includes
More than two magnets, polarity of each magnet close to reaction cup side are identical；Second group of magnets includes four or more magnetic assemblies, often
A magnetic assembly includes two magnets disposed in parallel, and the pole orientation of two magnets is identical in two magnetic assemblies of rear and front end,
Two magnet poles directions of remaining magnetic assembly are different；Third group of magnets includes three or more magnetic assemblies, and each magnetic assembly includes
Two magnet poles directions of two magnets disposed in parallel, the last one magnetic assembly are identical, two magnets of remaining magnetic assembly
Pole orientation is different, and the pole orientation of the two neighboring magnet of two neighboring magnetic assembly is identical；
The setting height of the magnetic assembly of third group of magnets gradually decreases and height is below the magnet and the of the first group of magnets
Height is arranged in the magnetic assembly of two group of magnets.
Preferably, two magnets of the first group of magnets are the pole N towards the polarity of reaction cup conveying assembly side；Second magnetic
Iron group is followed successively by N-N, S-N, N-S, S-S towards reaction cup conveying assembly magnet polarities from the direction close to the first group of magnets；The
Three group of magnets are followed successively by N-S, S-N, N-N towards reaction cup conveying assembly magnet polarities from the direction close to the second group of magnets.
Preferably, the pole orientation of magnet is vertical with reaction cup transmission direction.
Preferably, all magnetic assemblies of group of magnets are placed equidistant in the horizontal plane in magnet fixed plate.
Preferably, two magnets in magnetic assembly are arranged on magnet pin.
Preferably, magnet fixed block is installed on magnet pin.
Preferably, in the magnetic assembly of third group of magnets the latter magnetic assembly setting height than previous magnetic assembly setting
Highly low 2-4mm.
The beneficial effects of the present invention are: the analyzer magnetic cleaning separator that magnet unilateral side of the invention is arranged passes through rationally
Magnet arrangements and magnetic pole arrangement, so that magnetic bead is adsorbed on reaction cup wall of cup or is rotated in reaction cup so that cleaning magnetic bead
When the unbonded object that makes to be wrapped in conjugate release, preferably retain that a part of reactant in conjunction with magnetic bead, and
It ensure that the time during entire Magneto separate can be short as far as possible, can also finally guarantee cup after reaction cup enters measuring chamber
Interior magnetic bead can sufficiently be reacted with substrate, and save substrate solution.
Detailed description of the invention
Present invention will be further explained below with reference to the attached drawings and examples.
Fig. 1 is the three-dimensional view of the analyzer magnetic cleaning separator of magnet unilateral side arrangement of the present invention；
Fig. 2 is magnet arrangements and the magnetic pole row of the analyzer magnetic cleaning separator group of magnets of magnet unilateral side arrangement of the present invention
Cloth schematic diagram.
Appended drawing reference are as follows:
1- reaction cup transport assembly, 2- magnetic cleaning separating mechanism, the first group of magnets of 21-, the second group of magnets of 22-, third magnetic
Iron group, 24- magnet fixed plate, 25- magnet pin.
Specific embodiment
In conjunction with the accompanying drawings, the present invention is further explained in detail.These attached drawings are simplified schematic diagram, only with
Illustration illustrates basic structure of the invention, therefore it only shows the composition relevant to the invention.
Embodiment
The present embodiment provides a kind of analyzer magnetic cleaning separators of magnet unilateral side arrangement, as shown in Figure 1, comprising: use
In the reaction cup transport assembly 1 that reaction cup is carried out to horizontal transport, magnetic cleaning separating mechanism 2, for drawing and infusing from reaction cup
Enter the cleaning needle assemblies (the cleaning needle assemblies are the general components of this field, therefore are not drawn into figure) of cleaning solution, the magnetic
Washing separator structure 2 includes: several group of magnets, is conveyed positioned at 1 transmission belt the same side of reaction cup conveying assembly and along reaction cup
1 transmission direction of component arrangement；
As shown in Fig. 2, the group of magnets includes the first group of magnets 21, the second group of magnets 22 and third magnet set gradually
Group 23；First group of magnets 21 includes more than two magnets, and polarity of each magnet close to reaction cup side is identical；Second group of magnets
22 include four or more magnetic assemblies, and each magnetic assembly includes two magnets disposed in parallel, in two magnetic assemblies of rear and front end
The pole orientation of two magnets is identical, and two magnet poles directions of remaining magnetic assembly are different；Third group of magnets 23 includes three
The above magnetic assembly, each magnetic assembly include two magnets disposed in parallel, two magnet poles directions of the last one magnetic assembly
Identical, two magnet poles directions of remaining magnetic assembly are different, the pole orientation of the two neighboring magnet of two neighboring magnetic assembly
It is identical；
As shown in Fig. 2, the setting height of the magnetic assembly of the third group of magnets 23 gradually decreases and height is below first
Height, the latter in the magnetic assembly of the third group of magnets 23 is arranged in the magnetic assembly of the magnet of group of magnets 21 and the second group of magnets 22
The setting height of magnetic assembly is than the setting of the previous magnetic assembly low 2-4mm of height, such as 2mm, 3mm, 4mm, so that in reaction cup
Magnetic bead is effectively pulled down at reaction cup bottom of a cup.
The magnet realizes that magnetic force attracts so that magnetic bead is adsorbed on reaction cup wall of cup or is reacting with magnetic bead in reaction cup
Rotation in cup, the magnet of the first group of magnets are mainly that the magnetic bead of reaction cup is adsorbed on the wall of cup of magnetic assembly side, the
Two group of magnets make the magnetic bead in reaction cup swing along reaction cup by constantly transformation magnetic direction, effectively to clean magnetic bead,
Magnetic bead in reaction cup is mainly gradually pulled down at reaction cup bottom of a cup by third group of magnets, to guarantee that reaction cup enters measuring chamber
The magnetic bead in cup can sufficiently be reacted with substrate afterwards.
Preferably, the intermediate accommodating chamber for the reaction cup that the present embodiment uses is triangular prism or tetragonous bigger than rear end of front end face
Column, a side of triangular prism or the front end face face magnet of quadrangular front end, and it is close close to the reaction cup bottom of a cup of magnet
The height of the side of magnet 1-2mm low compared with other positions, cleaning needle are directed at the higher position of reaction cup bottom of a cup in imbibition, with
So that magnetic bead is gathered in the lower side of reaction cup bottom of a cup in cleaning separation process, cleaning needle is prevented to be drawn onto the magnetic bead in reaction cup.
Preferably, two magnets of the first group of magnets 21 are the pole N towards the polarity of 1 side of reaction cup conveying assembly；The
Two group of magnets 22 are followed successively by N-N, S-N towards 1 magnet polarities of reaction cup conveying assembly from the direction close to the first group of magnets 21,
N-S,S-S；Third group of magnets 23 is followed successively by from the direction close to the second group of magnets towards 1 magnet polarities of reaction cup conveying assembly
N-S,S-N,N-N。
As shown in Figure 1, the pole orientation of the magnet is vertical with reaction cup transmission direction, so that magnetic in magnet and reaction cup
The magnetic force of pearl, which attracts, to be maximized, and the washing effect of magnetic bead is enhanced.
As depicted in figs. 1 and 2, all magnetic assemblies of group of magnets are placed equidistant in the horizontal plane in magnet fixed plate 24.
As depicted in figs. 1 and 2, two magnets in the magnetic assembly are arranged on magnet pin 25.
Preferably, magnet fixed block is installed, for limiting the position of magnet on the magnet pin 25.
As shown in Fig. 2, the magnet of first group of magnets 21, the second group of magnets 22 and third group of magnets 23 is cylindricality.
Preferably, the cleaning needle assemblies include:
Cleaning needle unit, including needle guard, the mounting base of fixed cleaning needle；
Cleaning needle unit driving mechanism can after protruding into reaction cup to drive cleaning needle to protrude into or stretch out reaction cup
To carry out imbibition and fluid injection；
Cleaning needle wiper mechanism, to clean cleaning needle.
Preferably, the needle guard of the cleaning needle unit is equipped with spring, and cleaning needle is made to reach reaction cup bottom, net to blot
Cleaning solution in reaction cup prevents cleaning needle from excessively contacting that needle point is damaged in reaction cup bottom.
In application, reaction cup is delivered at the first group of magnets by reaction cup conveying assembly, magnet is by reaction cup at this
In magnetic bead be adsorbed on wall of cup side, then reaction cup is delivered at the second group of magnets, first sops up reaction with cleaning needle assemblies
Liquid injects cleaning solution, and then the primary then absorption of the every rotation of the magnetic bead in reaction cup and injection cleaning solution are primary, to guarantee to the greatest extent may be used
Extra reactant is cleaned out on magnetic bead more than energy, and finally reaction cup is delivered at third group of magnets, will be in reaction cup
Magnetic bead is pulled down at reaction cup bottom of a cup, to guarantee that it is anti-that the magnetic bead after reaction cup enters measuring chamber in cup can be carried out sufficiently with substrate
It answers, and substrate solution can be saved, finally sop up reaction by cleaning needle assemblies at the last one magnetic assembly of the group of magnets
Cleaning solution in cup.
Taking the above-mentioned ideal embodiment according to the present invention as inspiration, through the above description, relevant staff is complete
Various changes and amendments can be carried out without departing from the scope of the technological thought of the present invention' entirely.The technology of this invention
Property range is not limited to the contents of the specification, it is necessary to which the technical scope thereof is determined according to the scope of the claim.
Claims (7)
1. a kind of analyzer magnetic cleaning separator of magnet unilateral side arrangement, comprising: for reaction cup to be carried out horizontal transport
Reaction cup transport assembly (1), magnetic cleaning separating mechanism (2), for the cleaning needle assemblies of cleaning solution to be drawn and injected from reaction cup,
It is characterized in that, the magnetic cleaning separating mechanism (2) includes:
Several group of magnets are located at reaction cup conveying assembly (1) transmission belt the same side and along reaction cup conveying assembly (1) transmission side
To arrangement；
The group of magnets includes the first group of magnets (21), the second group of magnets (22) and third group of magnets (23) set gradually；The
One group of magnets (21) includes two magnets, and polarity of two magnets towards reaction cup conveying assembly (1) side is the pole N；Second
Group of magnets (22) includes four magnetic assemblies, and each magnetic assembly includes two magnets disposed in parallel, and the second group of magnets (22) is from leaning on
The direction of nearly first group of magnets (21) is followed successively by N-N, S-N, N-S, S-S towards reaction cup conveying assembly (1) magnet polarities；The
Three group of magnets (23) include three magnetic assemblies, each magnetic assembly include two magnets disposed in parallel, third group of magnets (23) from
Close to the direction of the second group of magnets, N-S, S-N, N-N are followed successively by towards reaction cup conveying assembly (1) magnet polarities；
The setting height of the magnetic assembly of the third group of magnets (23) gradually decreases and height is below the first group of magnets (21)
The magnetic assembly of magnet and the second group of magnets (22) setting height；
The cleaning needle assemblies include:
Cleaning needle unit, including cleaning needle, the mounting base of fixed cleaning needle；
Cleaning needle unit driving mechanism, to drive cleaning needle to protrude into or stretch out reaction cup；
Cleaning needle wiper mechanism, to clean cleaning needle.
2. the analyzer magnetic cleaning separator of magnet unilateral side arrangement according to claim 1, which is characterized in that the magnetic
The pole orientation of body is vertical with reaction cup transmission direction.
3. the analyzer magnetic cleaning separator of magnet unilateral side arrangement according to claim 2, which is characterized in that group of magnets
All magnetic assemblies be placed equidistant on magnet fixed plate (24) in the horizontal plane.
4. the analyzer magnetic cleaning separator of magnet unilateral side arrangement according to claim 3, which is characterized in that the magnetic
Two magnets on component are arranged on magnet pin (25).
5. the analyzer magnetic cleaning separator of magnet unilateral side arrangement according to claim 4, which is characterized in that the magnetic
Magnet fixed block is installed on iron pin (25).
6. the analyzer magnetic cleaning separator of magnet unilateral side arrangement according to claim 1-5, feature exist
In the magnet of first group of magnets (21), the second group of magnets (22) and third group of magnets (23) is cylindricality.
7. the analyzer magnetic cleaning separator of magnet unilateral side arrangement according to claim 1-5, feature exist
In setting height of the setting height of the latter magnetic assembly than previous magnetic assembly in the magnetic assembly of the third group of magnets (23)
Low 2-4mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710506691.6A CN107144690B (en) | 2017-06-28 | 2017-06-28 | A kind of analyzer magnetic cleaning separator of magnet unilateral side arrangement |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710506691.6A CN107144690B (en) | 2017-06-28 | 2017-06-28 | A kind of analyzer magnetic cleaning separator of magnet unilateral side arrangement |
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| Publication Number | Publication Date |
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| CN107144690A CN107144690A (en) | 2017-09-08 |
| CN107144690B true CN107144690B (en) | 2019-04-23 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710506691.6A Active CN107144690B (en) | 2017-06-28 | 2017-06-28 | A kind of analyzer magnetic cleaning separator of magnet unilateral side arrangement |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110082343B (en) * | 2018-01-25 | 2024-03-19 | 深圳市新产业生物医学工程股份有限公司 | Side suction assembly |
| CN110361527B (en) * | 2018-03-26 | 2023-04-28 | 成都深迈瑞医疗电子技术研究院有限公司 | Magnetic bead cleaning and separating device and chemiluminescence immunoassay analyzer |
| CN109433686A (en) * | 2018-12-27 | 2019-03-08 | 上海奥普生物医药有限公司 | Magnetic bead cleaning device and Full-automatic chemiluminescence immunoassay analysis meter |
| CN110672835A (en) * | 2019-09-26 | 2020-01-10 | 合肥运涛光电科技有限公司 | Magnetic separation mechanism for chemiluminescence immunoassay system |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0358948A2 (en) * | 1988-08-10 | 1990-03-21 | E.I. Du Pont De Nemours And Company | Method for automatically processing magnetic solid phase reagents |
| WO2000023807A1 (en) * | 1998-10-16 | 2000-04-27 | Bayer Corporation | Method and apparatus for wash, resuspension, recollection and localization of magnetizable particles in assays using magnetic separation technology |
| EP1202808A1 (en) * | 1999-07-19 | 2002-05-08 | Organon Teknika B.V. | Device and method for mixing magnetic particals with a fluid |
| CN206975043U (en) * | 2017-06-28 | 2018-02-06 | 苏州长光华医生物医学工程有限公司 | A kind of analyzer magnetic cleaning separator of the unilateral arrangement of magnet |
-
2017
- 2017-06-28 CN CN201710506691.6A patent/CN107144690B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0358948A2 (en) * | 1988-08-10 | 1990-03-21 | E.I. Du Pont De Nemours And Company | Method for automatically processing magnetic solid phase reagents |
| WO2000023807A1 (en) * | 1998-10-16 | 2000-04-27 | Bayer Corporation | Method and apparatus for wash, resuspension, recollection and localization of magnetizable particles in assays using magnetic separation technology |
| EP1202808A1 (en) * | 1999-07-19 | 2002-05-08 | Organon Teknika B.V. | Device and method for mixing magnetic particals with a fluid |
| CN206975043U (en) * | 2017-06-28 | 2018-02-06 | 苏州长光华医生物医学工程有限公司 | A kind of analyzer magnetic cleaning separator of the unilateral arrangement of magnet |
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| CN107144690A (en) | 2017-09-08 |
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