CN107129037A - It is quick to start the method that Anammox coupling denitrification cooperates with denitrification reactor - Google Patents

It is quick to start the method that Anammox coupling denitrification cooperates with denitrification reactor Download PDF

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Publication number
CN107129037A
CN107129037A CN201710362832.1A CN201710362832A CN107129037A CN 107129037 A CN107129037 A CN 107129037A CN 201710362832 A CN201710362832 A CN 201710362832A CN 107129037 A CN107129037 A CN 107129037A
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concentration
denitrification
reactor
cooperates
sludge
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CN107129037B (en
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秦玉洁
曹雁
韩彬
王桐屿
任君怡
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South China University of Technology SCUT
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    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/28Anaerobic digestion processes
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00Nature of the contaminant
    • C02F2101/10Inorganic compounds
    • C02F2101/16Nitrogen compounds, e.g. ammonia
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2305/00Use of specific compounds during water treatment
    • C02F2305/06Nutrients for stimulating the growth of microorganisms

Abstract

The invention discloses a kind of method that quick startup Anammox coupling denitrification cooperates with denitrification reactor, Anammox and denitrifying mixing sludge are used for seed sludge, and mixed volume ratio is 4:1, MLVSS ratio is 0.89:1, inoculum concentration is 1000mL, and water inlet uses NH4Cl and NaNO2For nitrogen source, NaHCO3And C6H12O6For carbon source.The water inlet is made after being contacted into water with sludge by the way of continuously inputting, and the gas for reacting generation is discharged from the steam vent of reactor head, and water outlet is flowed out by the downflow weir on reactor top.The inlet flow-patterm is adjusted to 7.5 ~ 8.0, and hydraulic detention time HRT is 10 14h, is run under normal temperature condition.The present invention realizes the collaboration denitrogenation of anaerobic ammonia oxidizing bacteria and denitrifying bacterium, and nitric efficiency is up to 95.1%.

Description

It is quick to start the method that Anammox coupling denitrification cooperates with denitrification reactor
Technical field
The present invention relates to wastewater biological denitrificaion field, be it is a kind of using Anammox and denitrifying mixing sludge to be inoculated with Sludge, while adding a small amount of glucose in water inlet quickly to start Anammox coupling denitrification collaboration denitrification reactor Method.
Background technology
Anaerobic ammonium oxidation technology for biological denitrification is the nearly two novel biological dinitrification technologies found during the last ten years.With traditional nitre Change denitrification denitrogenation technique to compare, anaerobic ammonia oxidation process has without additional carbon and oxygen supply power consumption, excess sludge production The advantages of amount is few.Anammox refers to that under anoxic conditions, anaerobic ammonia oxidizing bacteria is with NH4 +- N is electron donor, NO2 -- N is Electron acceptor, the process for the generation nitrogen that reacts.Its reaction equation is:
NH4 + + 1.32 NO2 - + 0.0066 HCO3 - + 0.13 H+ → 0.0066 CH2O0.5N0.15 +1.02 N2↑ + 0.26 NO3 - + 2.03 H2O (1)
Anammox reaction can produce a certain amount of NO3 -- N, causes total nitric efficiency to be ideally below 89%.In addition, anaerobism Ammoxidation is to the NO in water inlet2 --N/NH4 +- N proportion requirement is very strict, and is difficult to reach such essence in actual application True ratio, causes total nitric efficiency of practical application relatively low.Therefore using Anammox and denitrifying mixing sludge to connect Sludge is planted, a small amount of organic matter is added in water inlet, denitrifying bacterium is using organic matter as electron donor, and Anammox reaction is produced NO3 -- N is electron acceptor, and react generation nitrogen, so as to reduce the NO in water outlet3 -- N concentration, improves reactor Total nitric efficiency.
The content of the invention
It is an object of the invention to improve the nitric efficiency of anaerobic ammonia oxidation reactor, for the waste water of low ratio of carbon to ammonium, carry A kind of method that denitrogenation is cooperateed with for Anammox coupling denitrification.
To achieve the above object of the invention, this invention takes following technical scheme:
A kind of method that quick startup Anammox coupling denitrification cooperates with denitrification reactor, using Anammox and anti-nitre The mixing sludge of change is seed sludge, and mixed volume ratio is 4:1, MLVSS ratio is 0.89:1, inoculum concentration is 1000mL, enters hydromining Use NH4Cl and NaNO2For nitrogen source, NaHCO3 And C6H12O6For carbon source.
Further, the water inlet is made after being contacted into water with sludge by the way of continuously inputting, and reacts the gas of generation Discharged from the steam vent of reactor head, water outlet is flowed out by the downflow weir on reactor top.
Further, the inlet flow-patterm is adjusted to 7.5 ~ 8.0, and hydraulic detention time HRT is 10-14h, under normal temperature condition Operation.
Further, described water inlet pH value is reconciled using hydrochloric acid or alkali.
Further, the formula of the water inlet is:The NH4Cl and NaNO2Concentration be respectively 84 ~ 106 mg/L and 122 ~ 142 mg/L, the NaHCO3 And C6H12O6Concentration be respectively 1000 mg/L and 56 ~ 60 mg/L;Other things Matter includes the MgSO47H that concentration is 473mg/L2O, concentration are 180 mg/L CaCl2, concentration is 27 mg/L KH2PO4, concentration be respectively 1mL/L trace element I, trace element II, described micro- I is that concentration is 5000 mg/L EDTA or FeSO4·7H2O;Described trace element II is the ZnSO that concentration is 430 mg/L4·7H2O, concentration are 240 mg/ L GuSO5·H2O, concentration are 990 mg/L MnCl2·4H2O, concentration are 190 mg/L NiCl2·6H2O or concentration For 24 mg/L CoCl2·6H2O。
Compared with prior art, the present invention realizes the collaboration denitrogenation of anaerobic ammonia oxidizing bacteria and denitrifying bacterium, improves reactor Total nitric efficiency, nitric efficiency is up to more than 95.1%, easy to operate, cost is low.
Brief description of the drawings
The Anammox coupling denitrification experimental provision schematic diagram that Fig. 1 is used by the embodiment of the present invention.
Fig. 2 is method therefor of the embodiment of the present invention to NH4 +- N removal effect.
Fig. 3 is method therefor of the embodiment of the present invention to NO2 -- N removal effect.
Fig. 4 is method therefor of the embodiment of the present invention to TN removal effects and NO3 -- N situations of change.
Fig. 5 is method therefor of the embodiment of the present invention to Δ NO2 --N/ΔNH4 +- N and Δ NO3 --N/ΔNH4 +- N value changes feelings Condition.
In figure:1- intake pool 2- organic carbon source 3- peristaltic pump 4- sample tap 5- sludge area 6- packing layer 7- three-phase separates From device 8- discharge bay 9- gas buffers bottle.
Embodiment
Below in conjunction with Figure of description, the present invention will be further described, but scope of the present invention not office It is limited to the scope described in embodiment.
As shown in figure 1, the experimental provision that the present invention is used is as shown in Figure 1, reactor reacts for UBF Anammoxs Device, is made up of lucite, in cylinder, the main part of the reactor include successively from the bottom to top sludge area 5, packing layer 6, Three phase separator 7, peristaltic pump 3, organic carbon source 2, intake pool 1 are sequentially connected in reactor bottom, by peristaltic pump 3 to reactor Sludge area 5 intake, sample tap 4 is provided with sludge area 5.
Reactor total measurement (volume) is 3.94L, and dischargeable capacity is 2.1L, and a diameter of 8cm, total height is 66cm, in sludge area Top addition sponge filler prevents sludge loss, and reactor outer layer avoids influence of the light to anaerobic ammonia oxidizing bacteria with black cloth shading. Waste water is continuously pumped into by peristaltic pump from reactor bottom, is sufficiently mixed with sludge after reaction, and the gas of generation is from reactor top The steam vent in portion is expelled to gas buffer bottle 9, and water outlet flows out to discharge bay 8, inlet flow-patterm control by the downflow weir on reactor top 7.5~8.0, run under normal temperature condition.
Embodiment one
A kind of method that quick startup Anammox coupling denitrification cooperates with denitrification reactor, reactor water inlet is useless using simulation Water, adds NH4Cl and NaNO2As nitrogen source, concentration is respectively 84 ~ 106 mg/L and 122 ~ 142 mg/L, NaHCO3 With C6H12O6As carbon source, concentration is respectively 1000 mg/L and 56 ~ 60 mg/L, and other materials include MgSO47H2O473 Mg/L, CaCl2180 mg/L, KH2PO427 mg/L, micro- I 1mL/L and micro- II 1mL/L.Wherein micro member Plain I is:The mg/L of EDTA 5000 and FeSO4·7H2O 5000 mg/L.Micro- II is:ZnSO4·7H2O430 mg/L, GuSO5·H2O 240 mg/L, MnCl2·4H2O 990 mg/L, NiCl2·6H2O 190 mg/L, CoCl2·6H2O 24 mg/ L.Water inlet pH value is adjusted to 7.5~8.0 with hydrochloric acid.
Seed sludge uses Anammox and denitrifying mixing sludge, wherein, anaerobic ammonium oxidation sludge comes from this reality Test the UASB reactors that room one has run nearly 8 months, anoxic of the denitrification sludge from Guangzhou LiKau sewage treatment plants Pond.Mixed volume ratio is 4:1, MLVSS ratio is 0.89:1, inoculum concentration is 1000mL.
Implementation result:
As shown in accompanying drawing 2,3,4, the NH in water inlet4 +-N、NO2 -- N and NO3 -- N concentration respectively 84.3 ~ 105.8 mg/L, Fluctuated between 122.0 ~ 141.3 mg/L and 1.0 ~ 4.6 mg/L, and the NH in water outlet4 +-N、NO2 -- N and NO3 -- N concentration is distinguished Fluctuated between 0 ~ 11.3 mg/L, 0 ~ 11.4 mg/L and 0 ~ 16.5 mg/L.When adding a small amount of glucose in water inlet, ammonia nitrogen Clearance do not have because a small amount of glucose addition and reduce.NH in water outlet4 +- N concentration is passed through by 11.3 initial mg/L Spend 7 days and be reduced to 0.3 mg/L, the clearance of ammonia nitrogen increases to 99.7% by 88.6%.In the experiment of 45 days, ammonia nitrogen is averaged Clearance is up to 99.2%.Illustrate that the activity of anaerobic ammonia oxidizing bacteria does not have reduction.
As shown in Figure 5, Δ NO2 --N/ΔNH4 +- N values are fluctuated between 1.29 ~ 1.51, average delta NO2 --N/ΔNH4 +-N (It is set to X)It is worth for 1.36, is closer to theoretical value 1.32.And Δ NO3 --N/ΔNH4 +- N values are fluctuated between 0 ~ 0.15, average ΔNO3 --N/ΔNH4 +-N(It is set to Y)It is worth for 0.06, much smaller than theoretical value 0.26, illustrates denitrifying bacterium in reactor using having Machine carbon is electron donor, the NO that Anammox is generated3 -- N is converted into nitrogen, causes Y value to be less than theoretical value.Can by accompanying drawing 4 Know, TN removal efficiency is fluctuated between 89.8% ~ 99.9%, TN average removal efficiency is up to 95.1%, higher than Anammox The theoretical value 89.0% of reaction, illustrates that anaerobic ammonia oxidizing bacteria and denitrifying bacterium can well coexist, Anammox coupling denitrification Collaboration denitrification reactor starts successfully.
The above embodiment of the present invention is only intended to clearly illustrate example of the present invention, and is not to the present invention Embodiment restriction.For those of ordinary skill in the field, it can also make on the basis of the above description Other various forms of changes or variation.There is no necessity and possibility to exhaust all the enbodiments.It is all the present invention Any modifications, equivalent substitutions and improvements made within spirit and principle etc., should be included in the protection of the claims in the present invention Within the scope of.

Claims (5)

1. a kind of method that quick startup Anammox coupling denitrification cooperates with denitrification reactor, it is characterised in that:Using detesting Anaerobic ammonium oxidation and denitrifying mixing sludge are seed sludge, and mixed volume ratio is 4:1, MLVSS ratio is 0.89:1, inoculum concentration is 1000mL, water inlet uses NH4Cl and NaNO2For nitrogen source, NaHCO3 And C6H12O6For carbon source.
2. the method that quick startup Anammox coupling denitrification according to claim 1 cooperates with denitrification reactor, its It is characterised by:The water inlet makes after being contacted into water with sludge using continuously inputting by the way of, and the gas that reaction is generated is from reactor The steam vent discharge at top, water outlet is flowed out by the downflow weir on reactor top.
3. the method that quick startup Anammox coupling denitrification according to claim 1 cooperates with denitrification reactor, its It is characterised by:The inlet flow-patterm is adjusted to 7.5 ~ 8.0, and hydraulic detention time HRT is 10-14h, is run under normal temperature condition.
4. the method that quick startup Anammox coupling denitrification according to claim 3 cooperates with denitrification reactor, its It is characterised by:Described water inlet pH value is reconciled using hydrochloric acid or alkali.
5. the method that quick startup Anammox coupling denitrification according to claim 1 cooperates with denitrification reactor, institute The formula stated into water is:The NH4Cl and NaNO2Concentration be respectively 84 ~ 106 mg/L and 122 ~ 142 mg/L, it is described NaHCO3 And C6H12O6Concentration be respectively 1000 mg/L and 56 ~ 60 mg/L;It is 473mg/L that other materials, which include concentration, MgSO47H2O, concentration are 180 mg/L CaCl2, concentration be 27 mg/L KH2PO4, concentration be respectively the micro- of 1mL/L Secondary element I, trace element II, described micro- I is the EDTA or FeSO that concentration is 5000 mg/L4·7H2O;Described is micro- Secondary element II is the ZnSO that concentration is 430 mg/L4·7H2O, concentration are 240 mg/L GuSO5·H2O, concentration are 990 Mg/L MnCl2·4H2O, concentration are 190 mg/L NiCl2·6H2O or concentration are 24 mg/L CoCl2·6H2O。
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108103000A (en) * 2017-12-27 2018-06-01 齐鲁工业大学 A kind of growth promoter for improving anaerobic ammonia oxidizing bacteria and preparation method thereof
CN109607779A (en) * 2019-02-21 2019-04-12 合肥工业大学 A kind of method of anerobic sowage ammoxidation coupling solid phase denitrification efficient denitrification

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CN105174448A (en) * 2015-10-13 2015-12-23 沈阳建筑大学 Anaerobic ammonia oxidation and denitrification coupling device and controlled operation method thereof
CN106315844A (en) * 2016-09-27 2017-01-11 华南理工大学 ANAMMOX reactor and starting method thereof by inoculating mixed sludge

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US20050279702A1 (en) * 2004-06-18 2005-12-22 Hitachi Plant Engineering & Contruction Co., Ltd. Method for operating anaerobic oxidation vessel and anaerobic ammonium oxidation equipment
CN101007684A (en) * 2007-01-19 2007-08-01 华南理工大学 Anerobic ammoxidation and denitrification synergistic denitrification method for garbage leachate
CN102259976A (en) * 2011-05-23 2011-11-30 杭州师范大学 Method for quickly starting anaerobic ammonia oxidation reactor
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108103000A (en) * 2017-12-27 2018-06-01 齐鲁工业大学 A kind of growth promoter for improving anaerobic ammonia oxidizing bacteria and preparation method thereof
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