CN107090049A - Arborvitae total starches and its purposes in immunosuppressive drug is prepared - Google Patents
Arborvitae total starches and its purposes in immunosuppressive drug is prepared Download PDFInfo
- Publication number
- CN107090049A CN107090049A CN201610088573.3A CN201610088573A CN107090049A CN 107090049 A CN107090049 A CN 107090049A CN 201610088573 A CN201610088573 A CN 201610088573A CN 107090049 A CN107090049 A CN 107090049A
- Authority
- CN
- China
- Prior art keywords
- arborvitae
- total starches
- polysaccharide
- total
- content
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/13—Coniferophyta (gymnosperms)
- A61K36/14—Cupressaceae (Cypress family), e.g. juniper or cypress
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Polymers & Plastics (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Sustainable Development (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention belongs to the field of Chinese medicines, it is related to a kind of arborvitae total starches, the polysaccharide is from the former plant arborvitae of Tibetan medicine Xu bar（Sabina pingii var. wilsonii）The Water-soluble polysccharide of preparation is extracted in branches and leaves, the present invention is characterized to architectural features such as its sugared content, glucuronic acid content, protein content, molecular weight distribution, monose compositions；Through determination of activity, as a result show that described arborvitae polysaccharide has obvious anticomplementary action, 23~35 μ g/ml are reached to the half effective inhibition concentration of complement activation classical pathway, it is suitable with positive control medicine liquaemin activity；LPS can be significantly inhibited simultaneously stimulates lower Turnover of Mouse Peritoneal Macrophages to secrete NO level, shows that it has good antiinflammatory action；The arborvitae total starches of the present invention can be used for preparing the immunosupress related drugs such as anticomplement, anti-inflammatory.
Description
Technical field
The invention belongs to the field of Chinese medicines, it is related to a kind of plant polyose and its medicinal usage, and in particular to a kind of from arborvitae
（Sabina pingii var. wilsonii）It is middle extract prepare total starches and its in anticomplement, anti-inflammatory drug is prepared
Purposes.
Background technology
Eliminating external microorganism prior art discloses the normal Activate of complement system, removing damage or dead in human body
Cell and tissue, maintain body balance in play an important role.But the improper activation of the system can cause human body
The overreaction of immune system, causes the damage of human body itself normal structure, participates in rheumatoid arthritis, systemic red yabbi
The pathologic process of a variety of diseases such as sore, ARDS.Current clinically widely used glucocorticoid, ring phosphorus
Although the immunodepressant such as acid amides, the cry of certain animals of methylamine butterfly have certain therapeutic action to some diseases related to excess complement activation,
Due to the not single-minded complement inhibitor of such medicine, poor selectivity, prolonged application can reduce the defensive enginery of body, cause to resist
Infection ability declines, and easy scabies secondary infection and spreads hidden focus, generation multiple complications and side effect.Therefore clinically it is badly in need of
Efficiently, low toxicity, single-minded new complement inhibitor.
Natural polysaccharide is the main active monoid of Chinese herbal medicine, such as panaxan, GL-B, pachymaran, matrimony vine
Polysaccharide etc., is often reported with the important activity such as Immune-enhancing effect, antitumor, hypoglycemic, increasingly causes natural drug or health care
The attention of field of food researcher.And part Traditional Chinese Medicine of Qin gre lei polysaccharide, shown if cordate houttuynia polysaccharide, lithosperman etc. bright
Table in terms of aobvious anticomplement, anti-inflammatory activity, the severe inflammatory disease such as the ALI related to excess complement activation in preventing and treating
Reveal good curative effect（ZL201010022880.4, ZL201110284642.5）.
Arborvitae（Sabina pingii var. wilsonii）It is one of former plant of Tibetan medicine " Xu's bar ", is mainly distributed on
Height above sea level 2600-4900 meters of the provinces and regions such as the Hubei northwestward, Southern Shaanxi, SOUTH OF GANSU, Sichuan, Yunnan and Tibet alpine belt, with
Branches and leaves are used as medicine, with heat-clearing, clearing damp, removing toxic substances the effects such as, for treating pneumonia, rheumatic arthritis, rheumatoid arthritis etc.
Disease.Containing the composition such as volatile oil, flavones, Phenylpropanoid Glycosides, steroidal in document report arborvitae, but so far there is not yet work to its polysaccharide
Journal of Sex Research is reported.
The content of the invention
It is an object of the invention to provide a kind of water-soluble plant total starches and its purposes in immunosuppressive drug is prepared.
Especially arborvitae total starches and its purposes in anticomplement, anti-inflammatory drug is prepared.
Arborvitae total starches of the present invention are from the former plant arborvitae of Tibetan medicine Xu bar（Sabina pingii var.
wilsonii）A kind of Water-soluble polysccharide of preparation is extracted in branches and leaves, is made by following steps：Arborvitae branches and leaves are crushed, used
Ethanol grease removal, carries out water extraction, and alcohol precipitation after Aqueous extracts concentration, precipitation is freeze-dried after redissolving.
Arborvitae total starches produced by the present invention have following architectural feature：
Molecular weight distribution：Less than 25000 Da；Monose is constituted：Based on arabinose, galacturonic acid, galactolipin, glucose,
And contain a small amount of rhamnose, mannose, glucuronic acid；Sugared content is more than 55%, and glucuronic acid content is more than 8%, and protein content is small
In 2%.
Determined, as a result shown using cell in vitro haemolysis method in the present invention, described arborvitae total starches have obvious suppression
Excess complement activation effect processed, the IC of three batches batches of arborvitae total starches of measure50It is worth for the μ g/ml of 23 μ g/ml~35, with positive control
Medicine liquaemin（27.81μg/ml）It is active suitable；
Determined, as a result shown, described arborvitae total starches can significantly inhibit LPS and lure using macrophages secrete NO methods in the present invention
The peritoneal macrophage NO secretions led, with stronger antiphlogistic effects；
The present invention shows that described arborvitae total starches can enter one as good innate immunity inhibitor through determination of activity result
Step preparation prepares anticomplement, anti-inflammatory drug, for treating the inflammatory disease that excess complement activation or pathogenic microorganism trigger.
Brief description of the drawings
Fig. 1 is arborvitae polysaccharide efficient gel electrophoretogram.
Fig. 2 is influences of arborvitae total starches SP1, the SP2 to macrophages secrete NO.
Embodiment
Embodiment 1 prepares arborvitae total starches
Arborvitae branches and leaves are crushed, 1 is pressed with 95% ethanol:30 solid-liquid ratio carries out heating under reduced pressure backflow（55 DEG C, point 3 times, each 4h）
The dregs of a decoction after grease removal are volatilized, and 1 is pressed with water:50 solid-liquid ratio carries out heating under reduced pressure refluxing extraction（70 DEG C, point 3 times, each 5h）Will
Aqueous extracts are concentrated into 0.4g crude drugs/ml, plus 4 times of volumes 95% ethanol, filter after alcohol precipitation 24h, will be precipitated and be freezed after redissolving with water
Dry, obtain total starches；Technique, prepares three crowdes of arborvitae total starches SP1, SP2 and SP3 respectively according to this.
The structural characterization of the arborvitae total starches of embodiment 2
（1）Molecular weight distribution：
Arborvitae total starches SP3 is configured to the 0.3mg/ml aqueous solution, HPLC analyses are carried out：Using TSK-GEL GMPWXL gels
Post（300×7.6 mm）, mobile phase is deionized water, the mL/min of flow velocity 0.8,25 DEG C of column temperature, Detection wavelength 210nm；With phase
Many saccharides are analyzed with method, are shown by comparing spectrogram, the molecular weight of arborvitae polysaccharide is approximately less than 25000 Da；
（2）Monose is constituted：
8 mg polysaccharide samples are taken to add 1M TFA 2ml, 110 DEG C of heating 5h, centrifugation, supernatant is evaporated, and precipitation is washed three times with methanol；
Then PMP derivatizations are carried out：Acid hydrolysis products 2mldd-H2O dissolves, and takes 1ml to add 1ml PMP solution and the M of 1ml 0.3
NaOH, 70 DEG C of heating 2h hydrolysis.1ml 0.3M HCl are added after cooling to neutralize, and are added after chloroform is extracted 3 times and are discarded chloroform layer, so
HPLC analyses are carried out afterwards：Using Agilent extend C18 posts (250*4.6mm, 5 μm), PBS is used（Phosphate buffer）：
Acetonitrile elutes for eluent gradient, flow velocity 0.7ml/min, Detection wavelength 245nm；By with monose standard control, obtain monose
Composition and its relative amount（As shown in table 1）, the composition of three batches of arborvitae total monosaccharides is basically identical, mainly contains arabinose, half
Lactobionic acid, galactolipin, glucose, and containing a small amount of mannose, rhamnose and glucuronic acid；
The monose composition HPLC analysis results of 1 three batches of arborvitae total starches of table
（3）Sugared content：
Set up standard curve：Arabinose monose reference substance 3.8mg is weighed, is dissolved in water, is settled to 25.0ml molten as standard
Liquid, draws 0,0.5,0.25,0.15,0.1,0.05,0.02 ml standard liquids in rub oral examination tube respectively, plus distilled water water is extremely
0.5ml, then adds 5% phenol solution 0.5ml, adds concentrated sulfuric acid 2.5ml（Preferably liquid level is applied directly in the present embodiment
On）5min is acutely vibrated afterwards, 15min is placed, its absorption value is measured with spectrophotometric under 490nm, draws standard curve；
Determination sample solution：Arborvitae polysaccharide 3.0mg is weighed, is placed in 10ml volumetric flasks, scale, pipette samples liquid 0.2 is added water to
Ml volumes survey its absorption value in rub oral examination tube by above operating procedure, and it is 59.96% to calculate content by standard curve；
（4）Glucuronic acid content：
Set up standard curve：Galacturonic acid 1.9mg is weighed, is dissolved in water, 25.0ml is settled to as standard liquid, inhales respectively
0,0.04,0.08,0.12,0.24,0.40ml standard liquid is taken in rub oral examination tube, plus distilled water is to 0.50 ml, in ice bath
Precooling, adds 3.0 ml sodium tetraborates-sulphate reagent (sulfuric acid solution of 0.0125 M sodium tetraborates), vibration mixing, in boiling
5 min are heated in water-bath.It is cooled to ice bath after room temperature plus 50 μ l, 0.15% hydroxyl biphenyl solution (is matched somebody with somebody with 0.5% sodium hydroxide
System, refrigerator is preserved, and one month is stable), shake up, measure its absorption value with spectrophotometric under 520nm, draw standard curve；
Determination sample solution：Precision weighs the mg of arborvitae polysaccharide 1.2, is placed in 10 ml volumetric flasks, adds water to scale, pipette samples
The ml volumes of liquid 0.5 survey its absorption value in rub oral examination tube by above operating procedure, and calculating content by standard curve is
9.43%；
（5）Protein content：
The preparation of Coomassie brilliant G-250 solution：50mg Coomassie brilliant G-250s are weighed, are dissolved in the ethanol of 25ml 90%, are added
85%（W/V）Phosphoric acid 50ml, finally with distilled water constant volume to 500ml；
Set up regression equation：25mg bovine serum albumin(BSA)s are taken, are dissolved in 25ml distilled water, as 1.0mg/ml stoste, this is taken
Stoste obtains 0.5,0.25,0.125,0.0625,0.0312 mg/ml bovine serum albumin solution with water two-fold dilution, from each examination
The ml of draw solution 0.1 is put into 10 ml tool plug test tubes respectively in pipe, using 0.1 ml water as blank, adds 5 ml coomassies bright
Blue G-250 protein reagents, Gai Sai, mixing are placed and absorbance under 595 nm are surveyed after 5 min, draw standard curve；
Determination sample solution：Weigh arborvitae polysaccharide 3.0mg, be placed in 10 ml volumetric flasks, add water to scale, draw 0.1 ml in
In rub oral examination tube, it is 1.05% to survey its absorption value to calculate content by standard curve by above operating procedure.
The cell haemolysis method of embodiment 3 determines arborvitae total starches anti-complement activity
About 2.0mg arborvitae total starches are taken, it is accurately weighed, it is dissolved in 0.8ml BBS（Barbiturate buffer solution）In, two-fold dilution obtains
To 2.5,1.25,0.625,0.3125,0.1563,0.0781,0.0391,0.0195mg/ml each 0.4ml of sample solution, point
Blank control Xi Qu not be done in 0.2ml to 0.4ml BBS；Be separately added into remaining sample solution 0.2ml complement,
0.1ml hemolysin and 0.1ml 2% sheep red blood cell solution, take supernatant to survey absorption value under 405nm after centrifugation, deduct empty
The IC of the haemolysis inhibiting rate of three batches of arborvitae polysaccharide is calculated after the absorption value compareed in vain50Respectively SP1: 25.04μg/ml、SP2:
35.40μg/ml、SP3: 23.82μg/ml；The IC of positive control drug liquaemin50For 27.81 μ g/ml.
Embodiment 4 determines influence of the arborvitae total starches to macrophages secrete NO
4 days 5% sulphur glycollate culture mediums of intraperitoneal injection, Turnover of Mouse Peritoneal Macrophages is collected after 4 days before Balb/c mouse experiments,
Cell concentration is adjusted to 5 × 105/ ml kinds enter in 96 orifice plates, 200ul/ holes, are placed in adhere-wall culture 2h in cell culture incubator（37
℃, 5% CO2）；It is loaded according to following packet：(1) blank control group；（2）The independent treatment group of polysaccharide；（3）LPS treatment groups
（The final concentration of 1 μ g/ml of LPS）；(4) polysaccharide and LPS Combined Treatment groups；（5）Positive drug dexamethasone and LPS Combined Treatment groups
（DEX）；Every group of four multiple holes, are placed in incubator and cultivate 24 h, collect supernatant, and NO level is determined using Griess methods, is taken
The μ l of clear liquid 50 add 50 μ l Griess reagents, and room temperature places 10 min after mixing, it is fully reacted, 540 nm detection lights
Absorption value, NO standard curves are set up by standard liquid of sodium nitrite solution（0-200 μM of measurement range）, determine each experimental group
NO levels in cells and supernatant；
Test result indicates that, SP1, SP2 it is mono- processing when can significantly raise macrophage NO secretion levels, with Immune-enhancing effect live
Property；Meanwhile, SP1, SP2 can significantly inhibit the peritoneal macrophage NO secretions of LPS inductions, with stronger antiphlogistic effects；Knot
Fruit shows that SP1, SP2 can have dual regulation to function of peritoneal macrophages.
Claims (3)
1. arborvitae total starches, it is characterised in that the arborvitae total starches are water-soluble plant total starches, from arborvitae
（Sabina pingii var. wilsonii）, with following characteristics：
Molecular weight is less than 25000 Da；Total sugar content is not less than 55%, and glucuronic acid content is higher than 8%, and protein content is less than 2%；Monose
Composition contains rhamnose, mannose and glucuronic acid based on arabinose, galacturonic acid, galactolipin and glucose.
2. purposes of the arborvitae total starches as claimed in claim 1 in Complement inhibition medicine is prepared.
3. purposes of the arborvitae total starches as claimed in claim 1 in anti-inflammatory drug is prepared.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610088573.3A CN107090049B (en) | 2016-02-17 | 2016-02-17 | Arborvitae total starches and its preparing the purposes in immunosuppressive drug |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610088573.3A CN107090049B (en) | 2016-02-17 | 2016-02-17 | Arborvitae total starches and its preparing the purposes in immunosuppressive drug |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107090049A true CN107090049A (en) | 2017-08-25 |
| CN107090049B CN107090049B (en) | 2019-10-08 |
Family
ID=59646009
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610088573.3A Active CN107090049B (en) | 2016-02-17 | 2016-02-17 | Arborvitae total starches and its preparing the purposes in immunosuppressive drug |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107090049B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110051682A (en) * | 2018-01-18 | 2019-07-26 | 复旦大学 | Purposes of the Sabina Mill. plant polysaccharide in the drug that preparation prevents and treats viral acute lung injury |
| CN110498864A (en) * | 2018-05-17 | 2019-11-26 | 复旦大学 | Arborvitae polysaccharide and preparation method thereof and preparing the purposes in anticomplement medicament |
| CN111825775A (en) * | 2019-04-18 | 2020-10-27 | 复旦大学 | Cupressus sempervirens polysaccharide, preparation method thereof and application thereof in preparing antitumor drugs |
| CN116425897A (en) * | 2023-04-23 | 2023-07-14 | 青岛农业大学 | Rosa roxburghii polysaccharide, and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5773005A (en) * | 1995-06-09 | 1998-06-30 | Takahashi; Hidehiko | Purified flavonoid and diterpene 5α-reductase inhibitors from thuja orientalis for androgen-related diseases |
| CN102090989A (en) * | 2011-01-27 | 2011-06-15 | 中国人民解放军北京军区总医院 | Application of phellodendron polysaccharides for resisting ultraviolet radiation and preparation method of anti-ultraviolet sun-screening agent |
| CN105061615A (en) * | 2015-07-28 | 2015-11-18 | 华南理工大学 | Chinese arborvitae twig polysaccharide with antiviral and immunity-enhancing activity, and preparation method and application thereof |
-
2016
- 2016-02-17 CN CN201610088573.3A patent/CN107090049B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5773005A (en) * | 1995-06-09 | 1998-06-30 | Takahashi; Hidehiko | Purified flavonoid and diterpene 5α-reductase inhibitors from thuja orientalis for androgen-related diseases |
| CN102090989A (en) * | 2011-01-27 | 2011-06-15 | 中国人民解放军北京军区总医院 | Application of phellodendron polysaccharides for resisting ultraviolet radiation and preparation method of anti-ultraviolet sun-screening agent |
| CN105061615A (en) * | 2015-07-28 | 2015-11-18 | 华南理工大学 | Chinese arborvitae twig polysaccharide with antiviral and immunity-enhancing activity, and preparation method and application thereof |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110051682A (en) * | 2018-01-18 | 2019-07-26 | 复旦大学 | Purposes of the Sabina Mill. plant polysaccharide in the drug that preparation prevents and treats viral acute lung injury |
| CN110051682B (en) * | 2018-01-18 | 2022-01-18 | 复旦大学 | Application of polysaccharide of thuja in preparation of medicine for preventing and treating viral acute lung injury |
| CN110498864A (en) * | 2018-05-17 | 2019-11-26 | 复旦大学 | Arborvitae polysaccharide and preparation method thereof and preparing the purposes in anticomplement medicament |
| CN110498864B (en) * | 2018-05-17 | 2021-09-07 | 复旦大学 | Selaginella polysaccharide, preparation method thereof and application thereof in preparation of anticomplement medicines |
| CN111825775A (en) * | 2019-04-18 | 2020-10-27 | 复旦大学 | Cupressus sempervirens polysaccharide, preparation method thereof and application thereof in preparing antitumor drugs |
| CN111825775B (en) * | 2019-04-18 | 2021-11-02 | 复旦大学 | Cupressus sempervirens polysaccharide, preparation method thereof and application thereof in preparing antitumor drugs |
| CN116425897A (en) * | 2023-04-23 | 2023-07-14 | 青岛农业大学 | Rosa roxburghii polysaccharide, and preparation method and application thereof |
| CN116425897B (en) * | 2023-04-23 | 2024-03-29 | 青岛农业大学 | Rosa roxburghii polysaccharide, and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107090049B (en) | 2019-10-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102417544B (en) | Ziyang selenium-rich green tea selenium-containing polysaccharide, and preparation method and application thereof | |
| CN107090049A (en) | Arborvitae total starches and its purposes in immunosuppressive drug is prepared | |
| Aher et al. | Pharmacological study of Tinospora cordifolia as an immunomodulator | |
| Song et al. | Immunomodulatory effects of crude phenylethanoid glycosides from Ligustrum purpurascens | |
| CN101890084B (en) | Semen nigellae total glycoside extract and preparation method and application thereof | |
| CN106749729B (en) | A kind of Smilacina japonica polysaccharide and its preparation method and application | |
| CN1965894B (en) | Prunella vulgaris polysaccharide having immune activity, preparation method and application thereof | |
| CN100443498C (en) | Use of anti-inflammatory medicine for scheelite total saponin and its saponin compound | |
| CN100408594C (en) | Lonicera macranthoides active total saponin extract and its preparing method and use | |
| CN105267356B (en) | A kind of morinda root oligosacchride and preparation method thereof | |
| He et al. | Potential low toxic alternative for Na-Cl cotransporter inhibition: A diuretic effect and mechanism study of Pyrrosia petiolosa | |
| CN102716380B (en) | Compound acne-removal cream and production method thereof | |
| Wang et al. | Pine pollen polysaccharides promote cell proliferation and accelerate wound healing by activating the JAK2-STAT3 signaling pathway | |
| CN104877037B (en) | Separation and purification method, products and application of Christia vespertilionis polysaccharides | |
| CN107213239B (en) | Traditional Chinese medicine composition and preparation method and application thereof | |
| Fitri et al. | Immunostimulant Activity of Pegagan Embun Herbs Extract (Hydrocotyle sibthorpioides Lam.) with carbon clearance method towards male white mice | |
| CN111317791A (en) | Heat-clearing and stranguria-treating tablet and preparation method thereof | |
| CN102362877B (en) | Application of Pouzolzia extract | |
| Nanumala et al. | Evaluations of diuretic activity of methanolic extract of Physalis angulata L. leaves | |
| CN104820029A (en) | Content detection method for compound houttuynia cordata mixture | |
| CN102028722B (en) | Extract of effective parts of total saponins in pterocephalus hookeri as well as preparation method and application thereof | |
| CN103558303A (en) | Method for measuring content of oleanolic acid in glossy privet fruit and astragalus membranaceus healthy energy-strengthening preparation | |
| CN110404021B (en) | Rhizoma polygonati preparation and preparation method thereof | |
| CN103897010A (en) | Composition for treating psoriasis and preparation method thereof | |
| CN105770869A (en) | Medicinal composition for treating psoriasis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |