CN107032873A - Alleviate the microbial manure and preparation method of vegetables in greenhouse booth salt stress - Google Patents
Alleviate the microbial manure and preparation method of vegetables in greenhouse booth salt stress Download PDFInfo
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- CN107032873A CN107032873A CN201611225099.0A CN201611225099A CN107032873A CN 107032873 A CN107032873 A CN 107032873A CN 201611225099 A CN201611225099 A CN 201611225099A CN 107032873 A CN107032873 A CN 107032873A
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- CN
- China
- Prior art keywords
- microbial manure
- vegetables
- salt stress
- wall material
- soil
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Classifications
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/30—Layered or coated, e.g. dust-preventing coatings
Abstract
The invention discloses a kind of microbial manure for alleviating vegetables in greenhouse booth salt stress and preparation method thereof, using beneficial microbe and its secretion as core, using converted starch, cyclodextrin, humic acid and bentonite as wall material, the exploitation of microbial manure is carried out using spray drying process.Including following preparation steps：Step one, core prepares；Step 2, wall material prepares；Step 3, the preparation of microbial manure.The microbial manure of the present invention alleviates stress of the secondary salinization soil to vegetable growth, adds Soil Microorganism quantity, maintains the microecological balance of soil；And the secretion produced by microbial cultivation process is also together embedded in microbial manure, especially there are diseases prevention, disease-resistant, alleviation salt stress to crops seedling stage, promote plant growth, so as to reach the purpose for improving Quality and biomass of vegetables, and then leverage the promotion and application of the microbial manure product.
Description
Technical field
The present invention relates to a kind of microbial manure and preparation method for alleviating vegetables in greenhouse booth salt stress, microorganism belonging to genus work
Journey and technical field of plant nutrition.The exploitation of the microbial manure can alleviate crop salt stress with application, promote plant growth
Development, improves Quality and biomass of vegetables.
Background technology
Shandong is national maximum vegetable supply base, is also the cradle of booth vegetable technology.Develop booth vegetable
Main source as Shandong most peasants family agricultural incomes, canopy dish production turns into pillar production important in agricultural production
Industry.However as the fast development of canopy dish, the long-term culture underglass of earth's surface and unreasonable administration fertilizer show soil secondary salinization
As obvious, growing for vegetables is affected, and yield and quality declines.
Vegetable grower increases applying quantity of chemical fertilizer to pursue high yield and high profit, but is due to that its utilization rate is low, causes substantial amounts of
Salt ion can not effectively be absorbed by crop and remain in soil, cause soil salt ion concentration to increase, soil hardening, have
The adverse consequences such as machine matter declines, soil nutrient is out of proportion, more exacerbates the development of soil secondary salinization.
Greenhouse soil secondary salinization is the technical barrier of generally existing in domestic and international facility cultivation, is to cause greenhouse soil
The major reason of degeneration, yield of vegetables and Quality Down.And with the extension in canopy age, the total salt amount of soil be continuously increased and progressively to
Top layer is gathered, and different degrees of salt damage makes vegetables underproduction amplitude increase year by year.Therefore, in face of greenhouse soil secondary salinization this
Technical barrier, needs a kind of more preferable product i.e. microbial manure badly and alleviates salt stress to be aided with chemical fertilizer and organic fertilizer,
Improve the yield and quality of vegetables.
The content of the invention
The technical problems to be solved by the invention are：One kind is provided to delay warmhouse booth soil secondary salinization, alleviate salt
Stress, the microbial manure and preparation method for improving Quality and biomass of vegetables.
In order to solve the above technical problems, the technical solution adopted by the present invention is：
The microbial manure for the alleviation vegetables in greenhouse booth salt stress that the present invention is provided, with beneficial microbe and its secretion
For core, using biodegradable and environmentally friendly material as wall material, using spray drying process to beneficial microbe and secretion
Thing is embedded.Specifically coerced with screening obtained diseases prevention, disease-resistant, alleviation salt from the warmhouse booth secondary salinization soil of Shandong
Urgent, Soluble phosphorus, potassium decomposing, one or more beneficial microbes of promotion plant growth and its secretion are core, with converted starch, ring
Dextrin, humic acid and bentonite are wall material, and spray drying alleviate opening for the microbial manure of vegetables in greenhouse booth salt stress
Hair.
Beneficial microbe of the present invention is selected from acid-producing Klebsiella bacterium, bacillus subtilis, bacillus megaterium, glue
The one or more combinations frozen in sample bacillus.These microorganisms can be dissolved by fixed phosphorus, potassium in soil, promote to plant
Absorption of the thing to phosphorus, potassium；Reduce plant pair Na under condition of salt stress+Absolute absorption amount, increase to K+、Ca2+And Fe2+Suction
Receive；Effectively alleviate the salt stress that secondary salinization soil is produced to plant growth；Have to crops seedling stage disease well prevention,
Resistant function, promotes plant strain growth and the absorption to nutriment.
The present invention also provides a kind of method for preparing described microbial manure, comprises the following steps：
Step one, core prepares：Beneficial microbe is subjected to Liquid Culture, centrifuged under conditions of 8000rpm, bacterium mud
In -4 DEG C of preservations, retain supernatant, it is standby.
Step 2, wall material prepares：Converted starch, cyclodextrin, humic acid, bentonite are well mixed according to a certain percentage, with
The thalline supernatant of step one prepares wall material solution, and the moist heat sterilization under conditions of 121 DEG C is standby.
Step 3, the preparation of microbial manure：By the wall material solution of the core of step one and step 2 according to a certain percentage
Well mixed, spray drying obtains the microbial manure for alleviating vegetables in greenhouse booth salt stress.
The microorganism fertilizer preparation method for material of the present invention, implements step as follows：
Step one, core prepares：
(1) preparation of LB culture mediums
NaCl (10.0g), peptone (10.0g), dusty yeast (5.0g), H2O (1000ml), 121 DEG C of 20 points of moist heat sterilizations
Clock.
(2) beneficial microorganism Liquid Culture
Acid-producing Klebsiella bacterium, bacillus subtilis, bacillus megaterium, bacillusmusilaginosiengineering are respectively connected to LB liquid
In body culture medium, cultivated under conditions of 30 DEG C, 200rpm to early stage stationary phase, by zymotic fluid according to (2-5)：(2-5)：(0-
4)：The volume ratio of (0-4) is well mixed, and is centrifuged under conditions of 8000rpm, and bacterium mud retains supernatant in -4 DEG C of preservations, standby
With.
Step 2, wall material prepares
Converted starch, cyclodextrin, humic acid, bentonite are according to (8-10)：(2-4)：1：1 mass ratio is well mixed；Will
The wall material solution that well mixed coating material and the thalline culture supernatant compound concentration (W/V) of step one are 40-65%；
Moist heat sterilization under conditions of 121 DEG C, it is standby.
Step 3, the preparation of microbial manure
By core and wall material solution according to (V/V) 1-3：1 ratio is well mixed, and is 120-140 DEG C, is entered in EAT
Sample speed is that 15-20ml/min, leaving air temp are that spray drying obtains and alleviates the vegetables in greenhouse booth salt side of body under conditions of 60-70 DEG C
Urgent microbial manure.
The yield of gained microbial manure is 72-90%, and thalline survival rate is 85-93%, and viable count is up to 1012CFU/g,
Store at room temperature after two months, effective bacteria containing amount is still up to 1012CFU/g。
Beneficial effects of the present invention：
One or more beneficial microbes and its secretion are wrapped in biodegradable and environmentally friendly by the present invention
In material, microbial manure is prepared into.The microbial manure of the alleviation vegetables in greenhouse booth salt stress alleviates secondary salinization
Soil adds Soil Microorganism quantity to the stress of vegetable growth, maintains the microecological balance of soil；And microculture
During produced by secretion be also together embedded in microbial manure, especially have to crops seedling stage diseases prevention, it is disease-resistant, alleviate salt
Stress, the effect for promoting plant growth, so as to reach the purpose for improving Quality and biomass of vegetables, and then leverage micro- life
The promotion and application of thing fertilizer product.
The microorganism fertilizer for the alleviation vegetables in greenhouse booth salt stress that the present invention is developed is applied in canopy dish production process
Material, according to the uptake characters of vegetables and soil nutrient content situation rational application of fertilizers, as far as possible using without SO4 2-、Cl-Deng accessory ingredient
Fertilizer, the organic fertilizer for applying semi-decomposeds increase Soil Microorganism quantity, maintain the microecological balance in soil more, from
And reach reduction soil liquid salinity, delay soil secondary salinization, alleviate salt stress, improve Quality and biomass of vegetables most
Whole purpose.
Embodiment
Embodiment 1：
The microbial manure that the present embodiment is provided is as follows：
Acid-producing Klebsiella bacterium, bacillus subtilis are subjected to Liquid Culture respectively, zymotic fluid is according to 1:1 volume ratio is mixed
Close uniform, centrifuged under conditions of 8000rpm, preserve bacterium mud, and retain supernatant.By converted starch, cyclodextrin, humic acid,
Bentonite is according to 8：2：1：1 mass ratio is well mixed, and the wall material solution that thalline supernatant compound concentration (W/V) is 40%,
121 DEG C of moist heat sterilizations.By microorganism core with the wall material solution after sterilizing according to 1：1 volume ratio is well mixed, and is entering wind-warm syndrome
Degree is 120 DEG C, sample introduction speed is spray drying under conditions of 15ml/min, obtains the micro- life for alleviating vegetables in greenhouse booth salt stress
Thing fertilizer.
Embodiment 2：
The microbial manure that the present embodiment is provided is as follows：
Acid-producing Klebsiella bacterium, bacillus subtilis, bacillus megaterium are subjected to Liquid Culture respectively, zymotic fluid according to
3：3：4 volume ratio is well mixed, and is centrifuged under conditions of 8000rpm, preserves bacterium mud, and retain supernatant.By converted starch,
Cyclodextrin, humic acid, bentonite are according to 10：2：1：1 mass ratio is well mixed, and is with thalline supernatant compound concentration (W/V)
60% wall material solution, 121 DEG C of moist heat sterilizations.By microorganism core with the wall material solution after sterilizing according to 1:1 volume ratio is mixed
Close uniform, EAT be 140 DEG C, sample introduction speed be spray drying under conditions of 15ml/min, obtain and alleviate warmhouse booth
The microbial manure of vegetables salt stress.
Embodiment 3：
The microbial manure that the present embodiment is provided is as follows：
Acid-producing Klebsiella bacterium, bacillus subtilis, bacillusmusilaginosiengineering are subjected to Liquid Culture respectively, zymotic fluid is pressed
According to 3：3：4 volume ratio is well mixed, and is centrifuged under conditions of 8000rpm, preserves bacterium mud, and retain supernatant.Denaturation is formed sediment
Powder, cyclodextrin, humic acid, bentonite are according to 8：4：1：1 mass ratio is well mixed, with thalline supernatant compound concentration (W/V)
For 50% wall material solution, 121 DEG C of moist heat sterilizations.By microorganism core with the wall material solution after sterilizing according to 3：1 volume ratio
It is well mixed, EAT be 120 DEG C, sample introduction speed be spray drying under conditions of 20ml/min, obtain that to alleviate greenhouse big
The microbial manure of canopy vegetables salt stress.
Embodiment 4：
The microbial manure that the present embodiment is provided is as follows：
Acid-producing Klebsiella bacterium, bacillus subtilis, bacillus megaterium, bacillusmusilaginosiengineering are subjected to liquid respectively
Culture, zymotic fluid is according to 2：2：3：3 volume ratio is well mixed, and is centrifuged under conditions of 8000rpm, preserves bacterium mud, and retain
Supernatant.By converted starch, cyclodextrin, humic acid, bentonite according to 10：4：1：1 mass ratio is well mixed, with thalline supernatant
Liquid compound concentration (W/V) is 50% wall material solution, 121 DEG C of moist heat sterilizations.By microorganism core and the wall material solution after sterilizing
According to 3：1 volume ratio is well mixed, EAT be 140 DEG C, sample introduction speed be spray drying under conditions of 20ml/min,
Obtain the microbial manure for alleviating vegetables in greenhouse booth salt stress.
Embodiment 5：
By the microbial manure of the technical program embodiment 1-4 alleviation vegetables in greenhouse booth salt stresses prepared, with secondary
The cucumber planted in salinization soil is for studying thing, the volume increase effect of the method test product of conventional fertilizer application compatibility test fertilizer
Really.The microbial manure of the alleviation vegetables in greenhouse booth salt stress of the present invention is poured onto Cucumber root by way of pouring root with water,
Whole growth period applies the microbial manure three times.Each group experiment effect of increasing production is shown in Table 1.
Table 1 alleviates effect of increasing production table of the microbial manure to cucumber of vegetables in greenhouse booth salt stress
Result above shows the microbial manure of the alleviation vegetables in greenhouse booth salt stress of the present invention to secondary salinization soil
The cucumber planted in earth has obvious production-increasing function.
Embodiment 6：
By the microbial manure of the technical program embodiment 1-4 alleviation vegetables in greenhouse booth salt stresses prepared, with secondary
The tomato planted in salinization soil is for studying thing, the volume increase effect of the method test product of conventional fertilizer application compatibility test fertilizer
Really.The microbial manure of the alleviation vegetables in greenhouse booth salt stress of the present invention is poured onto tomato root by way of pouring root with water,
Whole growth period applies the microbial manure three times.Each group experiment effect of increasing production is shown in Table 2.
Table 2 alleviates effect of increasing production table of the microbial manure to tomato of vegetables in greenhouse booth salt stress
Result above shows the microbial manure of the alleviation vegetables in greenhouse booth salt stress of the present invention to secondary salinization soil
The tomato planted in earth has obvious production-increasing function.
Embodiment 7：
By the microbial manure of the technical program embodiment 1-4 alleviation vegetables in greenhouse booth salt stresses prepared, with secondary
The eggplant planted in salinization soil is for studying thing, the volume increase effect of the method test product of conventional fertilizer application compatibility test fertilizer
Really.The microbial manure of the alleviation vegetables in greenhouse booth salt stress of the present invention is poured onto eggplant root by way of pouring root with water,
Whole growth period applies the microbial manure three times.Each group experiment effect of increasing production is shown in Table 3.
Table 3 alleviates effect of increasing production table of the microbial manure to eggplant of vegetables in greenhouse booth salt stress
Result above shows the microbial manure of the alleviation vegetables in greenhouse booth salt stress of the present invention to secondary salinization soil
The eggplant planted in earth has obvious production-increasing function.
Embodiment 8：
By the microbial manure of the technical program embodiment 1-4 alleviation vegetables in greenhouse booth salt stresses prepared, with secondary
The green pepper planted in salinization soil is for studying thing, the volume increase effect of the method test product of conventional fertilizer application compatibility test fertilizer
Really.The microbial manure of the alleviation vegetables in greenhouse booth salt stress of the present invention is poured onto green pepper root by way of pouring root with water,
Whole growth period applies the microbial manure three times.Each group experiment effect of increasing production is shown in Table 4.
Table 4 alleviates effect of increasing production table of the microbial manure to green pepper of vegetables in greenhouse booth salt stress
Result above shows the microbial manure of the alleviation vegetables in greenhouse booth salt stress of the present invention to secondary salinization soil
The green pepper planted in earth has obvious production-increasing function.
Certainly, described above is not limitation of the present invention, and the present invention is also not limited to the example above, the art
Those of ordinary skill, the present invention essential scope in, the variations, modifications, additions or substitutions made, should all belong to the present invention
Protection domain.
Claims (3)
1. a kind of microbial manure for alleviating vegetables in greenhouse booth salt stress, it is characterised in that with beneficial microbe and its secretion
Thing is core, using converted starch, cyclodextrin, humic acid and bentonite as wall material.
2. microbial manure according to claim 1, it is characterised in that beneficial microbe be selected from acid-producing Klebsiella bacterium,
One or more combinations in bacillus subtilis, bacillus megaterium, bacillusmusilaginosiengineering.
3. prepare the method for the microbial manure described in any of the above-described claim, it is characterised in that comprise the following steps：
Step one, core prepares：Beneficial microbe is subjected to Liquid Culture, centrifuged under conditions of 8000rpm, bacterium mud is in -4 DEG C
Preserve, retain supernatant, it is standby；
Step 2, wall material prepares：Converted starch, cyclodextrin, humic acid, bentonite are well mixed according to a certain percentage, with step
One thalline supernatant prepares wall material solution, and the moist heat sterilization under conditions of 121 DEG C is standby；
Step 3, the preparation of microbial manure：The core of step one is mixed according to a certain percentage with the wall material solution of step 2
Uniformly, spray drying obtains the microbial manure for alleviating vegetables in greenhouse booth salt stress.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611225099.0A CN107032873A (en) | 2016-12-27 | 2016-12-27 | Alleviate the microbial manure and preparation method of vegetables in greenhouse booth salt stress |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611225099.0A CN107032873A (en) | 2016-12-27 | 2016-12-27 | Alleviate the microbial manure and preparation method of vegetables in greenhouse booth salt stress |
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| CN107032873A true CN107032873A (en) | 2017-08-11 |
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| CN201611225099.0A Pending CN107032873A (en) | 2016-12-27 | 2016-12-27 | Alleviate the microbial manure and preparation method of vegetables in greenhouse booth salt stress |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110156541A (en) * | 2019-07-03 | 2019-08-23 | 上海诺同农业科技有限公司 | A kind of restorative procedure of secondary salinization soil |
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| CN106242840A (en) * | 2016-08-29 | 2016-12-21 | 贵阳开磷化肥有限公司 | A kind of apple tree Special slow release fertilizer |
-
2016
- 2016-12-27 CN CN201611225099.0A patent/CN107032873A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101717301A (en) * | 2009-11-12 | 2010-06-02 | 北京理工大学 | Capsule type microbial fertilizer and preparation method thereof |
| CN103086799A (en) * | 2013-02-04 | 2013-05-08 | 广西田园生化股份有限公司 | Fertilizer-mix pesticide |
| CN104557312A (en) * | 2015-01-14 | 2015-04-29 | 河北春潮生物科技有限公司 | High-nutrient microbial bacterium composite fertilizer and production method thereof |
| CN106242840A (en) * | 2016-08-29 | 2016-12-21 | 贵阳开磷化肥有限公司 | A kind of apple tree Special slow release fertilizer |
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| CN110156541A (en) * | 2019-07-03 | 2019-08-23 | 上海诺同农业科技有限公司 | A kind of restorative procedure of secondary salinization soil |
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Application publication date: 20170811 |