CN107022465A - The method that lichee OPC suppresses AGEs in aging beer - Google Patents

The method that lichee OPC suppresses AGEs in aging beer Download PDF

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Publication number
CN107022465A
CN107022465A CN201710353668.8A CN201710353668A CN107022465A CN 107022465 A CN107022465 A CN 107022465A CN 201710353668 A CN201710353668 A CN 201710353668A CN 107022465 A CN107022465 A CN 107022465A
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Prior art keywords
beer
lichee
opc
ages
aging
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CN201710353668.8A
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Chinese (zh)
Inventor
吴茜
陈媛媛
柳志杰
周梦舟
胡勇
徐宁
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Hubei University of Technology
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Hubei University of Technology
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12H—PASTEURISATION, STERILISATION, PRESERVATION, PURIFICATION, CLARIFICATION OR AGEING OF ALCOHOLIC BEVERAGES; METHODS FOR ALTERING THE ALCOHOL CONTENT OF FERMENTED SOLUTIONS OR ALCOHOLIC BEVERAGES
    • C12H1/00—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages
    • C12H1/12—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages without precipitation
    • C12H1/14—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages without precipitation with non-precipitating compounds, e.g. sulfiting; Sequestration, e.g. with chelate-producing compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12H—PASTEURISATION, STERILISATION, PRESERVATION, PURIFICATION, CLARIFICATION OR AGEING OF ALCOHOLIC BEVERAGES; METHODS FOR ALTERING THE ALCOHOL CONTENT OF FERMENTED SOLUTIONS OR ALCOHOLIC BEVERAGES
    • C12H1/00—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages
    • C12H1/12—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages without precipitation
    • C12H1/16—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages without precipitation by physical means, e.g. irradiation

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Distillation Of Fermentation Liquor, Processing Of Alcohols, Vinegar And Beer (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Suppress AGEs method in aging beer the present invention relates to a kind of lichee OPC, draft beer supersonic wave cleaning machine is handled into froth breaking, to avoid the measurement error of volumetric errors and fluorescence measurement between sample；Prepare without it is any poison, it is safe and reliable, while having the lichee OPC solution of inoxidizability；It is added in the beer of defoaming treatment, seals；In the incubator for being put into 50 DEG C, accelerate its aging, it is to avoid prolonged slow aging under normal temperature, to extend beer shelf life；Survey AGEs content.To aging beer effect significantly, aging beer AGEs contents are 216~705 μ g/kg, inhibiting rate 48.33%~6.94% to the present invention；And without it is any poison, it is safe and reliable, while the effects such as having anti-oxidant.

Description

The method that lichee OPC suppresses AGEs in aging beer
Technical field
The invention belongs to technical field of food science, and in particular to a kind of lichee OPC suppresses in aging beer AGEs application process.
Background technology
Advanced glycation endproducts (Advanced Glycation End Products, AGEs) are by reduced sugar carbonyl With the free amine group of the macromolecular substances such as amino acid or protein, lipid or nucleic acid, through under the conditions of non-enzymatic and being condensed, again The one group of stable dead end product produced after row, cracking, oxidative modification.Numerous studies show：AGEs and diabetes, artery are athero- A variety of chronic diseases such as hardening, senile dementia are closely related.Therefore, finding AGEs inhibitor has important Research Significance.Ammonia Base guanidine (AG) is a kind of now widely studied AGEs formation inhibitor, but AG has larger side effect to human body, such as causes stomach Function of intestinal canal is disorderly, destroys PFT and causes vasculitis etc..
OPC (procyanidins, PCs) as flavone compound one kind, be it is a kind of have it is extremely strong anti-oxidant The natural dietary supplements of activity and radical scavenging activity.Lichee is subtropical fruit, originates in SOUTHERN CHINA, with Guangdong, extensively Based on the cultivation of the ground such as west, Fujian, Taiwan, together it is referred to as the big fruit in the southern part of the country four with banana, pineapple, longan.Seminar's early-stage Study Show, lichee is in addition to pulp is delicious, and pericarp rich in polyphenol compounds such as OPC, anthocyanin, flavones because equally depositing In huge Development volue, primary bioactivity includes anti-oxidant, antibacterial, reducing blood lipid, anti-trioxypurine etc..
In excitation wavelength it is 370nm to aging beer, launch wavelength is that 440nm locates, and detection AGEs contents are 705 μ g/kg, Its content is high, have impact on the nutrition and health care and security of beer, have impact on shelf life.Thus needing to work out can effectively reduce old Change the method for AGEs content in beer.
The content of the invention
The purpose of the present invention is to be directed to above-mentioned present situation, it is desirable to provide one kind can be reduced during beer storage because aging is produced AGEs, this method improves the nutrition and health care and security of beer, while can extend the lichee original flower of the shelf life of beer The method that blue or green element suppresses AGEs in aging beer.
The implementation of the object of the invention is that the method that lichee OPC suppresses AGEs in aging beer is specific to walk Suddenly it is：
1) draft beer is taken, supersonic wave cleaning machine eliminates the bubble of beer, and water temperature is controlled at 20 DEG C；
2) 0.02~0.5mg/ml lichee OPC solution is prepared；
3) by step 2) the lichee OPC solution prepared is added to step 1) in treated beer, fully mix, And seal；
4) by step 3) sealedly beer be put into 50 DEG C of incubator, place 2~8 days；
5) it is 370nm in excitation wavelength, launch wavelength is that 440nm locates measurement through step 4) AGEs contains in the beer of placement Amount.
The present invention in draft beer plus lichee OPC solution, lichee OPC without it is any poison, it is safe and reliable, together When the effects such as there is inoxidizability；Draft beer first handles froth breaking through supersonic wave cleaning machine, and the volume between sample can be avoided to miss The measurement error of difference and fluorescence measurement；Sealed again in draft beer plus after lichee OPC solution, be put into 50 DEG C of incubator In, accelerate its aging, the beer slow aging prolonged at normal temperatures of inhibiting can be simulated, for extending beer shelf Phase.
To aging beer effect significantly, aging beer AGEs contents are 216~705 μ g/kg, inhibiting rate to the present invention 48.33%~6.94%；And without it is any poison, it is safe and reliable, while the effects such as having anti-oxidant.
Embodiment
Draft beer supersonic wave cleaning machine is handled froth breaking by the present invention, preferably completely still；Preparation can without any murder by poisoning, safety Lean on, while having the lichee OPC solution of inoxidizability；It is added in the beer of defoaming treatment, seals；It is put into 50 DEG C Incubator in, accelerate its aging, it is to avoid prolonged slow aging under normal temperature, to extend beer shelf life；Survey containing for AGEs Amount.The present invention in draft beer plus lichee OPC solution, lichee OPC without it is any poison, it is safe and reliable, have simultaneously The effects such as having anti-oxidant；Draft beer first handles froth breaking through supersonic wave cleaning machine, can avoid volumetric errors between sample and glimmering The measurement error of photo measure；Seal, in the incubator for being put into 50 DEG C, swashed again in draft beer plus after lichee OPC solution Hair wavelength is 370nm, and launch wavelength is that AGEs contents are surveyed at 440nm.
Described lichee OPC, purity >=99%.Described lichee OPC, be using ethanol as Extraction solvent, By the OPC in AB-8 macroreticular resin separation and Extraction litchi rinds.
The present invention is described in detail with specific embodiment below.
Embodiment 1
1) draft beer is taken, supersonic wave cleaning machine eliminates the bubble of beer, and water temperature is controlled at 20 DEG C；
2) 0.5mg/ml lichee OPC solution is prepared；
3) by step 2) the lichee OPC solution prepared is added to step 1) in treated beer, fully mix, And seal；
4) by step 3) sealedly beer be put into 50 DEG C of incubator, place 2 days；
5) it is 370nm in excitation wavelength, launch wavelength is that 440nm locates measurement through step 4) AGEs contains in the beer of placement Amount, AGEs contents are 216 μ g/kg.
Embodiment 2
1) draft beer is taken, supersonic wave cleaning machine eliminates the bubble of beer, and water temperature is controlled at 25 DEG C；
2) 0.5mg/ml lichee OPC solution is prepared；
3) by step 2) the lichee OPC solution prepared is added to step 1) in treated beer, fully mix, And seal；
4) by step 3) sealedly beer be put into 50 DEG C of incubator, place 5 days；
5) it is 370nm in excitation wavelength, launch wavelength is that 440nm locates measurement through step 4) AGEs contains in the beer of placement Amount, AGEs contents are 367 μ g/kg.
Embodiment 3
1) draft beer is taken, supersonic wave cleaning machine eliminates the bubble of beer, and water temperature is controlled at 25 DEG C；
2) 0.5mg/ml lichee OPC solution is prepared；
3) by step 2) the lichee OPC solution prepared is added to step 1) in treated beer, fully mix, And seal；
4) by step 3) sealedly beer be put into 50 DEG C of incubator, place 8 days；
5) it is 370nm in excitation wavelength, launch wavelength is that 440nm locates measurement through step 4) AGEs contains in the beer of placement Amount, AGEs contents are 517 μ g/kg.
Embodiment 4
1) draft beer is taken, supersonic wave cleaning machine eliminates the bubble of beer, and water temperature is controlled at 20 DEG C；
2) 0.2mg/ml lichee OPC solution is prepared；
3) by step 2) the lichee OPC solution prepared is added to step 1) in treated beer, fully mix, And seal；
4) by step 3) sealedly beer be put into 50 DEG C of incubator, place 2 days；
5) it is 370nm in excitation wavelength, launch wavelength is that 440nm locates measurement through step 4) AGEs contains in the beer of placement Amount, AGEs contents are 338 μ g/kg.
Embodiment 5
1) draft beer is taken, supersonic wave cleaning machine eliminates the bubble of beer, and water temperature is controlled at 20 DEG C；
2) 0.2mg/ml lichee OPC solution is prepared；
3) by step 2) the lichee OPC solution prepared is added to step 1) in treated beer, fully mix, And seal；
4) by step 3) sealedly beer be put into 50 DEG C of incubator, place 5 days；
5) it is 370nm in excitation wavelength, launch wavelength is that 440nm locates measurement through step 4) AGEs contains in the beer of placement Amount, AGEs contents are 435 μ g/kg.
Embodiment 6
1) draft beer is taken, supersonic wave cleaning machine eliminates the bubble of beer, and water temperature is controlled at 20 DEG C；
2) 0.2mg/ml lichee OPC solution is prepared；
3) by step 2) the lichee OPC solution prepared is added to step 1) in treated beer, fully mix, And seal；
4) by step 3) sealedly beer be put into 50 DEG C of incubator, place 8 days；
5) it is 370nm in excitation wavelength, launch wavelength is that 440nm locates measurement through step 4) AGEs contains in the beer of placement Amount, AGEs contents are 589 μ g/kg.
Embodiment 7
1) draft beer is taken, supersonic wave cleaning machine eliminates the bubble of beer, and water temperature is controlled at 20 DEG C；
2) 0.02mg/ml lichee OPC solution is prepared；
3) by step 2) the lichee OPC solution prepared is added to step 1) in treated beer, fully mix, And seal；
4) by step 3) sealedly beer be put into 50 DEG C of incubator, place 2 days；
5) it is 370nm in excitation wavelength, launch wavelength is that 440nm locates measurement through step 4) AGEs contains in the beer of placement Amount, AGEs contents are 389 μ g/kg.
Embodiment 8
1) draft beer is taken, supersonic wave cleaning machine eliminates the bubble of beer, and water temperature is controlled at 20 DEG C；
2) 0.02mg/ml lichee OPC solution is prepared；
3) by step 2) the lichee OPC solution prepared is added to step 1) in treated beer, fully mix, And seal；
4) by step 3) sealedly beer be put into 50 DEG C of incubator, place 5 days；
5) it is 370nm in excitation wavelength, launch wavelength is that 440nm locates measurement through step 4) AGEs contains in the beer of placement Amount, AGEs contents are 537 μ g/kg.
Embodiment 9
1) draft beer is taken, supersonic wave cleaning machine eliminates the bubble of beer, and water temperature is controlled at 20 DEG C；
2) 0.02mg/ml lichee OPC solution is prepared；
3) by step 2) the lichee OPC solution prepared is added to step 1) in treated beer, fully mix, And seal；
4) by step 3) sealedly beer be put into 50 DEG C of incubator, place 8 days；
5) it is 370nm in excitation wavelength, launch wavelength is that 440nm locates measurement through step 4) AGEs contains in the beer of placement Amount, AGEs contents are 705 μ g/kg.
Make to be not added with the check experiment of lichee OPC solution for the applicant of embodiment 1,4,7, it is specific as follows：
1) draft beer is taken, supersonic wave cleaning machine eliminates the bubble of beer, and water temperature is controlled at 20 DEG C；
4) by step 1) de-soak beer is put into 50 DEG C of incubator, places 2 days；
5) it is 370nm in excitation wavelength, launch wavelength is that 440nm locates measurement through step 4) AGEs contains in the beer of placement Amount, AGEs contents are 418 μ g/kg.
The applicant of embodiment 2,5,8 has been made to be not added with the check experiment of lichee OPC solution, it is specific as follows：
1) draft beer is taken, supersonic wave cleaning machine eliminates the bubble of beer, and water temperature is controlled at 20 DEG C；
4) by step 1) de-soak beer is put into 50 DEG C of incubator, places 5 days；
5) it is 370nm in excitation wavelength, launch wavelength is that 440nm locates measurement through step 4) AGEs contains in the beer of placement Amount, AGEs contents are 586 μ g/kg.
The applicant of embodiment 3,6,9 has been made to be not added with the check experiment of lichee OPC solution, it is specific as follows：
1) draft beer is taken, supersonic wave cleaning machine eliminates the bubble of beer, and water temperature is controlled at 20 DEG C；
4) by step 1) de-soak beer is put into 50 DEG C of incubator, places 8 days；
5) it is 370nm in excitation wavelength, launch wavelength is that 440nm locates measurement through step 4) AGEs contains in the beer of placement Amount, wherein AGEs contents are 761 μ g/kg.
The AGEs contents of embodiment 1,2,3,4,5,6,7,8,9 compared with the AGEs contents of control group, embodiment 1,2,3, 4th, 5,6,7,8,9 inhibiting rate be respectively 48.33%, 37.37%, 32.06%, 19.14%, 25.77%, 22.60%, 6.94%th, 8.36%, 7.36%.Thus illustrate that lichee OPC solution can effectively suppress the AGEs contents in aging beer, Particularly lichee OPC solution concentration is 0.5mg/ml, is placed 2 days, optimal to the AGEs inhibitions in aging beer.

Claims (3)

1. the method that lichee OPC suppresses AGEs in aging beer, it is characterised in that；Comprise the following steps that：
1) draft beer is taken, supersonic wave cleaning machine eliminates the bubble of beer, and water temperature is controlled at 20 DEG C；
2) 0.02~0.5mg/ml lichee OPC solution is prepared；
3) by step 2) the lichee OPC solution prepared is added to step 1) in treated beer, fully mix, and it is close Envelope；
4) by step 3) sealedly beer be put into 50 DEG C of incubator, place 2~8 days；
5) excitation wavelength be 370nm, launch wavelength be 440nm locate measurement through step 4) placement beer in AGEs content.
2. the lichee OPC according to claims 1 suppresses AGEs application process, its feature in aging beer It is；Lichee OPC, purity >=99%.
3. the method that the lichee OPC according to claims 1 suppresses AGEs in aging beer, it is characterised in that； The lichee OPC is using ethanol as Extraction solvent, by the former cyanine in AB-8 macroreticular resin separation and Extraction litchi rinds Element.
CN201710353668.8A 2017-05-18 2017-05-18 The method that lichee OPC suppresses AGEs in aging beer Pending CN107022465A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108315203A (en) * 2018-05-03 2018-07-24 湖北工业大学 A kind of brewing method of low AGEs vinegars
CN108634006A (en) * 2018-05-03 2018-10-12 湖北工业大学 A kind of preparation method of low AGEs fermented bean curd

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CN104557838A (en) * 2014-12-11 2015-04-29 惠州学院 Method for extracting proantho cyanidins from litchi peels

Patent Citations (2)

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CN103070400A (en) * 2012-07-17 2013-05-01 华中农业大学 Use of lotus procyanidin as advanced glycosylation end product formation inhibitor
CN104557838A (en) * 2014-12-11 2015-04-29 惠州学院 Method for extracting proantho cyanidins from litchi peels

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108315203A (en) * 2018-05-03 2018-07-24 湖北工业大学 A kind of brewing method of low AGEs vinegars
CN108634006A (en) * 2018-05-03 2018-10-12 湖北工业大学 A kind of preparation method of low AGEs fermented bean curd
CN108315203B (en) * 2018-05-03 2021-05-28 湖北工业大学 Brewing method of low AGEs vinegar
CN108634006B (en) * 2018-05-03 2021-10-15 湖北工业大学 Preparation method of low AGEs fermented bean curd

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