CN106996926A - Tumour cell mitosis process biomechanical characterization monitoring device and monitoring method - Google Patents
Tumour cell mitosis process biomechanical characterization monitoring device and monitoring method Download PDFInfo
- Publication number
- CN106996926A CN106996926A CN201710214332.3A CN201710214332A CN106996926A CN 106996926 A CN106996926 A CN 106996926A CN 201710214332 A CN201710214332 A CN 201710214332A CN 106996926 A CN106996926 A CN 106996926A
- Authority
- CN
- China
- Prior art keywords
- cell
- detector
- measured
- mitosis
- biomechanics characteristic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
Abstract
The present invention relates to tumour cell mitosis process biomechanical characterization monitoring device and monitoring method, including three-dimensional fine-tuning section objective table, the cell physiological condition controlling device being located on three-dimensional fine-tuning section objective table and the cell incubation device in cell physiological condition controlling device, cell sample to be measured is provided with the cell incubation device, positioned at the position sensing sensor detector and the transducer tip of biomechanics characteristic detector of cell sample side to be measured, the sensor of the biomechanics characteristic detector is connected with biomechanics characteristic detector, first laser device and speculum are provided with above the cell incubation device, object lens are sequentially provided with below the cell incubation device, dichroic sheet, filter plate and convergent lens, second laser is additionally provided with below the object lens, ccd detector is provided with below the convergent lens to gather image information, the present invention can realize the real-time monitoring of mitosis process different phase cyto-mechanics feature.
Description
Technical field：
The present invention relates to a kind of tumour cell mitosis process biomechanical characterization monitoring device and monitoring method.
Background technology：
Tumour cell mitosis is the significant process of cell cycle, and its failure may result in cell death and chromosome is non-
Ortholoidy, causes cell cycle imbalance and tumour to be formed.Cell mitogen process is along with cytoskeletal structure, cell shape
The change of state and the change of mechanical characteristics.The change relative straightforward of mitosis Procedure Cell form, it is possible to use it is micro- into
As technology is observed, and although cell biomechanics characteristic is had been found in division, differentiation, migration, and tumour growth etc.
Played an important role in correlated process, but the biomechanics characteristic that cell is directly measured on single living cell has certain skill
Art difficulty and challenge.
The content of the invention：
It is an object of the invention to provide a kind of tumour cell mitosis process biomechanical characterization monitoring device and monitoring side
Method, the tumour cell mitosis process biomechanical characterization monitoring device and monitoring method are reasonable in design, can be real using it
The real-time monitoring of existing silk fission process different phase tumour cell mechanical characteristics.
Tumour cell mitosis process biomechanical characterization monitoring device of the present invention, it is characterised in that：It is micro- including three-dimensional
Adjust objective table, the cell physiological condition controlling device being located on three-dimensional fine-tuning section objective table and positioned at the control of cell physiological condition
Cell sample to be measured, the position positioned at cell sample side to be measured are provided with cell incubation device in device, the cell incubation device
Put the transducer tip of sensitive sensor detector and biomechanics characteristic detector, the sensing of the biomechanics characteristic detector
Device is connected with biomechanics characteristic detector, and first laser device and speculum, the cell are provided with above the cell incubation device
It is sequentially provided with below couveuse below object lens, dichroic sheet, filter plate and convergent lens, the object lens and is additionally provided with second laser,
Ccd detector is provided with below the convergent lens to gather image information, ccd detector and the position sensing sensor detector
Collection signal be connected with data collection and analysis control device.
Further, above-mentioned biomechanics characteristic detector is located on a three-dimensional regulation control device.
Further, above-mentioned cell physiological condition controlling device is a closed transparent vessel, the closed transparent vessel
Interior temperature-adjustable, humidity.
Further, above-mentioned position sensing sensor detector is PSD position sensing sensor detectors.
The monitoring method carried out using tumour cell mitosis process biomechanical characterization monitoring device, the tumour is thin
Born of the same parents' mitosis process biomechanical characterization monitoring device includes three-dimensional fine-tuning section objective table, is located on three-dimensional fine-tuning section objective table
Cell physiological condition controlling device and the cell incubation device in cell physiological condition controlling device, the cell incubation device
It is interior to be detected provided with cell sample to be measured, the position sensing sensor detector positioned at cell sample side to be measured and biomechanics characteristic
The transducer tip of device, the sensor of the biomechanics characteristic detector is connected with biomechanics characteristic detector, described thin
It is provided with above born of the same parents' couveuse below first laser device and speculum, the cell incubation device and is sequentially provided with object lens, dichroic sheet, filtering
It is additionally provided with below second laser, the convergent lens provided with ccd detector to gather below piece and convergent lens, the object lens
Image information, collection signal and the data collection and analysis control device of ccd detector and the position sensing sensor detector connect
Connect；Monitoring method specific steps：
(1) cell sample to be measured is positioned on cell incubation device, cell incubation device is then placed in the control of cell physiological condition
In device；
(2) cell physiological condition controlling device is adjusted, the surrounding environment of cell sample to be measured is in required experiment condition；
(3) by adjusting the optically focused scope of object lens, and cell sample to be measured is observed using ccd detector, obtains and wait to see
Survey the picture rich in detail of cell；
(4) by biomechanics characteristic detector and its three-dimensional regulation control device, by the sensing of biomechanics characteristic detector
Device end is placed in directly over cell placement device, but transducer tip is not yet contacted with epicyte；
(5) first laser device is opened, the hot spot on speculum, calibrating position sensitive sensor detector is adjusted；
(6) by adjusting three-dimensional regulation control device, biomechanics characteristic detector sensor end is made to be in cell physiological bar
It is stable 5-10 minutes in the testing sample environment that part control device is regulated and controled, it is ensured that it and the environment residing for cell sample to be measured
Unanimously；
(7) second laser is opened, the laser beam for allowing its laser to send can be irradiated on cell sample to be measured through object lens, reverse
Fluorescence signal passes through object lens, and through dichroic sheet, filter plate, convergent lens finally reach ccd detector and data collection and analysis control
Device processed, is realized to cell sample mitosis state observation to be measured；
(8) when observe cyto-chromatin condense into chromosome, i.e. cell be in mitosis prophase M1；
(9) by three-dimensional fine-tuning section objective table, by cell to be measured move to biomechanics characteristic detector sensor end just under
Side；
(10) adjust three-dimensional fine-tuning section objective table, make epicyte to be measured and biomechanics characteristic detector sensor end away from
From about several microns；
(11) keep biomechanics characteristic detector sensor end height constant, pass through position sensing sensor detector and data
Collection analysis control device, obtains the biomechanical characterization of mitosis prophase M1；
(12) the mitotic state of cell sample to be measured is monitored in real time using ccd detector；
(13) when observing nuclear membrane rupture, i.e., cell is in mitosis prometaphase M2；
(14) keep biomechanics characteristic detector sensing end highly constant, adopted by position sensing sensor detector and data
Set analysis control device, obtains the biomechanical characterization in mitosis prometaphase M2；
(15) the mitotic state of cell sample to be measured is monitored in real time using ccd detector；
(16) when observing that cell chromosome is distributed in the equatorial plane, i.e., cell is in mitosis metaphase M3；
(17) keep biomechanics characteristic detector sensing end highly constant, adopted by position sensing sensor detector and data
Set analysis control device, obtains the biomechanical characterization of mitosis metaphase M3；
(18) the mitotic state of cell sample to be measured is monitored in real time using ccd detector；
(19) when observing that cell chromosome is moved to the two poles of the earth, i.e., cell is in mitosis anaphase M4；
(20) keep biomechanics characteristic detector sensing end highly constant, adopted by position sensing sensor detector and data
Set analysis control device, obtains the biomechanical characterization of mitosis anaphase M4.
By above step, that is, realize the reality changed to tumour cell mitosis process different phase cell biomechanics
When monitor.
The present invention can realize the real-time monitoring of mitosis process different phase cyto-mechanics feature, Jin Ershen using it
Enter to understand during cell mitogen the factor such as skeleton structure change, intracellular external environment to the driving of fission process, control and
Influence, while the Biomechanical Mechanism cognition for the related life process of cell provides a kind of new method.
Brief description of the drawings：
Fig. 1 is the organigram of the present invention；
Fig. 2 is each stage schematic diagram of cell sample to be measured；
1st, position sensing sensor detector；2nd, speculum；3rd, first laser device；4th, biomechanics characteristic detector；41st, it is three-dimensional to adjust
Save control device；5th, cell sample to be measured；6th, cell incubation device；7th, cell physiological condition controlling device；8th, three-dimensional fine-tuning section is carried
Thing platform；9th, object lens；10th, dichroic sheet；11st, second laser；12nd, filter plate；13rd, convergent lens；14th, ccd detector；15、
Data collection and analysis control device.
Embodiment：
The present invention will be further described in detail with reference to the accompanying drawings and detailed description.
Tumour cell mitosis process biomechanical characterization monitoring device of the present invention include three-dimensional fine-tuning section objective table 8,
The cell physiological condition controlling device 7 that is located on three-dimensional fine-tuning section objective table 8 and in cell physiological condition controlling device
Cell sample 5 to be measured, the position sensing positioned at cell sample side to be measured are provided with cell incubation device 6, the cell incubation device 6
The transducer tip of sensor detector 1 and biomechanics characteristic detector 4, the sensor of the biomechanics characteristic detector with
Biomechanics characteristic detector is connected, and first laser device 3 and speculum 2 are provided with above the cell incubation device, and the cell is incubated
Educate the lower section of device 6 and be sequentially provided with object lens 9, dichroic sheet 10, filter plate 12 and convergent lens 13, the lower section of object lens 9 is additionally provided with second
Laser 11, the lower section of convergent lens 13 is provided with ccd detector 14 to gather image information, the ccd detector 14 and position
The collection signal for putting sensitive sensor detector 1 is connected with data collection and analysis control device 15.
Further, for the ease of regulation, above-mentioned biomechanics characteristic detector 4 is located at a three-dimensional regulation control device 41
On.
Further, above-mentioned cell physiological condition controlling device 7 is a closed transparent vessel, the closed transparent vessel
Interior temperature-adjustable, humidity.
Further, above-mentioned position sensing sensor detector is PSD position sensing sensor detectors.
First laser device 3 is laser diode, and it needs to be connected in computer gathering line, biomechanics characteristic detector bag
Biomethanics sensing measurement and data acquisition, control are included, it needs to be connected in computer gathering line, cell physiological condition control dress
Put including CO2 and temperature control unit, ccd detector and data collection and analysis control device are to realize above-mentioned corresponding module work(
The extent control of energy, the computer of data acquisition.
The monitoring method carried out using tumour cell mitosis process biomechanical characterization monitoring device, the tumour is thin
Born of the same parents' mitosis process biomechanical characterization monitoring device includes three-dimensional fine-tuning section objective table, is located on three-dimensional fine-tuning section objective table
Cell physiological condition controlling device and the cell incubation device in cell physiological condition controlling device, the cell incubation device
It is interior to be detected provided with cell sample to be measured, the position sensing sensor detector positioned at cell sample side to be measured and biomechanics characteristic
The transducer tip of device, the sensor of the biomechanics characteristic detector is connected with biomechanics characteristic detector, described thin
It is provided with above born of the same parents' couveuse below first laser device and speculum, the cell incubation device and is sequentially provided with object lens, dichroic sheet, filtering
It is additionally provided with below second laser, the convergent lens provided with ccd detector to gather below piece and convergent lens, the object lens
Image information, collection signal and the data collection and analysis control device of ccd detector and the position sensing sensor detector connect
Connect；Monitoring method specific steps：
(1) cell sample to be measured is positioned on cell incubation device, cell incubation device is then placed in the control of cell physiological condition
In device；
(2) cell physiological condition controlling device is adjusted, the surrounding environment of cell sample to be measured is in required experiment condition；
(3) by adjusting the optically focused scope of object lens, and cell sample to be measured is observed using ccd detector, obtains and wait to see
Survey the picture rich in detail of cell；
(4) by biomechanics characteristic detector and its three-dimensional regulation control device, by the sensing of biomechanics characteristic detector
Device end is placed in directly over cell placement device, but transducer tip is not yet contacted with epicyte；
(5) first laser device is opened, the hot spot on speculum, calibrating position sensitive sensor detector is adjusted；
(6) by adjusting three-dimensional regulation control device, biomechanics characteristic detector sensor end is made to be in cell physiological bar
It is stable 5-10 minutes in the testing sample environment that part control device is regulated and controled, it is ensured that it and the environment residing for cell sample to be measured
Unanimously；
(7) second laser is opened, the laser beam for allowing its laser to send can be irradiated on cell sample to be measured through object lens, reverse
Fluorescence signal passes through object lens, and through dichroic sheet, filter plate, convergent lens finally reach ccd detector and data collection and analysis control
Device processed, is realized to cell sample mitosis state observation to be measured；
(8) when observe cyto-chromatin condense into chromosome, i.e. cell be in mitosis prophase M1；
(9) by three-dimensional fine-tuning section objective table, by cell to be measured move to biomechanics characteristic detector sensor end just under
Side；
(10) adjust three-dimensional fine-tuning section objective table, make epicyte to be measured and biomechanics characteristic detector sensor end away from
From about several microns；
(11) keep biomechanics characteristic detector sensor end height constant, pass through position sensing sensor detector and data
Collection analysis control device, obtains the biomechanical characterization of mitosis prophase M1；
(12) the mitotic state of cell sample to be measured is monitored in real time using ccd detector；
(13) when observing nuclear membrane rupture, i.e., cell is in mitosis prometaphase M2；
(14) keep biomechanics characteristic detector sensing end highly constant, adopted by position sensing sensor detector and data
Set analysis control device, obtains the biomechanical characterization in mitosis prometaphase M2；
(15) the mitotic state of cell sample to be measured is monitored in real time using ccd detector；
(16) when observing that cell chromosome is distributed in the equatorial plane, i.e., cell is in mitosis metaphase M3；
(17) keep biomechanics characteristic detector sensing end highly constant, adopted by position sensing sensor detector and data
Set analysis control device, obtains the biomechanical characterization of mitosis metaphase M3；
(18) the mitotic state of cell sample to be measured is monitored in real time using ccd detector；
(19) when observing that cell chromosome is moved to the two poles of the earth, i.e., cell is in mitosis anaphase M4；
(20) keep biomechanics characteristic detector sensing end highly constant, adopted by position sensing sensor detector and data
Set analysis control device, obtains the biomechanical characterization of mitosis anaphase M4.
By above step, that is, realize the reality changed to tumour cell mitosis process different phase cell biomechanics
When monitor.
The present invention can realize the real-time monitoring of mitosis process different phase cyto-mechanics feature, Jin Ershen using it
Enter to understand during cell mitogen the factor such as skeleton structure change, intracellular external environment to the driving of fission process, control and
Influence, while the Biomechanical Mechanism cognition for the related life process of cell provides a kind of new method.
The foregoing is only presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, should all belong to the covering scope of the present invention.
Claims (5)
1. a kind of tumour cell mitosis process biomechanical characterization monitoring device, it is characterised in that：Including three-dimensional fine-tuning section
Objective table, the cell physiological condition controlling device being located on three-dimensional fine-tuning section objective table and positioned at cell physiological condition controlling device
In cell incubation device, in the cell incubation device provided with cell sample to be measured, positioned at cell sample side to be measured position it is quick
Feel the transducer tip of sensor detector and biomechanics characteristic detector, the sensor of the biomechanics characteristic detector with
Biomechanics characteristic detector is connected, and first laser device and speculum, the cell incubation are provided with above the cell incubation device
It is sequentially provided with below device below object lens, dichroic sheet, filter plate and convergent lens, the object lens and is additionally provided with second laser, it is described
Below convergent lens provided with ccd detector to gather image information, ccd detector and the position sensing sensor detector is adopted
Collection signal is connected with data collection and analysis control device.
2. tumour cell mitosis process biomechanical characterization monitoring device according to claim 1, it is characterised in that：
The biomechanics characteristic detector is located on a three-dimensional regulation control device.
3. tumour cell mitosis process biomechanical characterization monitoring device according to claim 1, it is characterised in that：
The cell physiological condition controlling device is temperature-adjustable, humidity in a closed transparent vessel, the closed transparent vessel.
4. tumour cell mitosis process biomechanical characterization monitoring device according to claim 1, it is characterised in that：
The position sensing sensor detector is PSD position sensing sensor detectors.
5. a kind of monitoring method that use tumour cell mitosis process biomechanical characterization monitoring device is carried out, its feature exists
In：The tumour cell mitosis process biomechanical characterization monitoring device includes three-dimensional fine-tuning section objective table, is located at three-dimensional
Cell physiological condition controlling device on fine adjustment objective table and the cell incubation device in cell physiological condition controlling device,
Cell sample to be measured, the position sensing sensor detector positioned at cell sample side to be measured and life are provided with the cell incubation device
The transducer tip of material resources property detector, sensor and the biomechanics characteristic of the biomechanics characteristic detector are detected
Device is connected, and is provided with above the cell incubation device below first laser device and speculum, the cell incubation device and is sequentially provided with thing
It is additionally provided with below second laser, the convergent lens and is provided with below mirror, dichroic sheet, filter plate and convergent lens, the object lens
Ccd detector is to gather image information, the collection signal of ccd detector and the position sensing sensor detector and data acquisition
Analysis Control Unit is connected；Monitoring method specific steps：
(1) cell sample to be measured is positioned on cell incubation device, cell incubation device is then placed in the control of cell physiological condition
In device；
(2) cell physiological condition controlling device is adjusted, the surrounding environment of cell sample to be measured is in required experiment condition；
(3) by adjusting the optically focused scope of object lens, and cell sample to be measured is observed using ccd detector, obtains and wait to see
Survey the picture rich in detail of cell；
(4) by biomechanics characteristic detector and its three-dimensional regulation control device, by the sensing of biomechanics characteristic detector
Device end is placed in directly over cell placement device, but transducer tip is not yet contacted with epicyte；
(5) first laser device is opened, the hot spot on speculum, calibrating position sensitive sensor detector is adjusted；
(6) by adjusting three-dimensional regulation control device, biomechanics characteristic detector sensor end is made to be in cell physiological bar
It is stable 5-10 minutes in the testing sample environment that part control device is regulated and controled, it is ensured that it and the environment residing for cell sample to be measured
Unanimously；
(7) second laser is opened, the laser beam for allowing its laser to send can be irradiated on cell sample to be measured through object lens, reverse
Fluorescence signal passes through object lens, and through dichroic sheet, filter plate, convergent lens finally reach ccd detector and data collection and analysis control
Device processed, is realized to cell sample mitosis state observation to be measured；
(8) when observe cyto-chromatin condense into chromosome, i.e. cell be in mitosis prophase M1；
(9) by three-dimensional fine-tuning section objective table, by cell to be measured move to biomechanics characteristic detector sensor end just under
Side；
(10) adjust three-dimensional fine-tuning section objective table, make epicyte to be measured and biomechanics characteristic detector sensor end away from
From about several microns；
(11) keep biomechanics characteristic detector sensor end height constant, pass through position sensing sensor detector and data
Collection analysis control device, obtains the biomechanical characterization of mitosis prophase M1；
(12) the mitotic state of cell sample to be measured is monitored in real time using ccd detector；
(13) when observing nuclear membrane rupture, i.e., cell is in mitosis prometaphase M2；
(14) keep biomechanics characteristic detector sensing end highly constant, adopted by position sensing sensor detector and data
Set analysis control device, obtains the biomechanical characterization in mitosis prometaphase M2；
(15) the mitotic state of cell sample to be measured is monitored in real time using ccd detector；
(16) when observing that cell chromosome is distributed in the equatorial plane, i.e., cell is in mitosis metaphase M3；
(17) keep biomechanics characteristic detector sensing end highly constant, adopted by position sensing sensor detector and data
Set analysis control device, obtains the biomechanical characterization of mitosis metaphase M3；
(18) the mitotic state of cell sample to be measured is monitored in real time using ccd detector；
(19) when observing that cell chromosome is moved to the two poles of the earth, i.e., cell is in mitosis anaphase M4；
(20) keep biomechanics characteristic detector sensing end highly constant, adopted by position sensing sensor detector and data
Set analysis control device, obtains the biomechanical characterization of mitosis anaphase M4；
By above step, that is, realize the real-time prison changed to tumour cell mitosis process different phase cell biomechanics
Survey.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710214332.3A CN106996926A (en) | 2017-04-01 | 2017-04-01 | Tumour cell mitosis process biomechanical characterization monitoring device and monitoring method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710214332.3A CN106996926A (en) | 2017-04-01 | 2017-04-01 | Tumour cell mitosis process biomechanical characterization monitoring device and monitoring method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106996926A true CN106996926A (en) | 2017-08-01 |
Family
ID=59435112
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710214332.3A Pending CN106996926A (en) | 2017-04-01 | 2017-04-01 | Tumour cell mitosis process biomechanical characterization monitoring device and monitoring method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106996926A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0223918A2 (en) * | 1985-11-26 | 1987-06-03 | International Business Machines Corporation | Method and atomic force microscope for imaging surfaces with atomic resolution |
| CN105572044A (en) * | 2016-01-11 | 2016-05-11 | 福建师范大学 | Method and device for obtaining biophysical characteristics of ultra-diffraction limit cell membrane micro-structure |
-
2017
- 2017-04-01 CN CN201710214332.3A patent/CN106996926A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0223918A2 (en) * | 1985-11-26 | 1987-06-03 | International Business Machines Corporation | Method and atomic force microscope for imaging surfaces with atomic resolution |
| CN105572044A (en) * | 2016-01-11 | 2016-05-11 | 福建师范大学 | Method and device for obtaining biophysical characteristics of ultra-diffraction limit cell membrane micro-structure |
Non-Patent Citations (3)
| Title |
|---|
| JPK INSTRUMENTS: "NanoWizard 3 user manual", 《NANOTECHNOLOGY FOR LIFE SCIENCE》 * |
| LEICA: "leica TCS SP8", 《谷歌》 * |
| NINGCHENG JIANG等: "Monitoring the elasticity changes of HeLa cells during mitosis by atomic force microscopy", 《谷歌，HTTPS://WWW.SPIEDIGITALLIBRARY.ORG/CONFERENCE-PROCEEDINGS-OF-SPIE/10024/1002427/MONITORING-THE-ELASTICITY-CHANGES-OF-HELA-CELLS-DURING-MITOSIS-BY/10.1117/12.2245537.SHORT?SSO=1》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101363839B (en) | Non-damage micrometering system for observating biological sample with fluorescent | |
| CN101351735B (en) | Device and method for capturing image of a sample originating from organism | |
| CN111398231A (en) | Scanning detection system based on diamond NV color center | |
| CN103983381B (en) | System and method for testing single particle adhesive force and electric quantity under vacuum condition | |
| CN104181131B (en) | Infrared modulated luminescence generated by light two-dimensional imaging light path is automatically positioned calibrating installation | |
| EP3312590B1 (en) | A non-invasive method for measuring absolute water content of a leaf | |
| CN107356619B (en) | A kind of micro-beam X-ray quickly positions and calibrating installation and its application method | |
| CN104469356A (en) | Image sensor characteristic parameter measuring and analyzing system | |
| CN105300943A (en) | Microscope integrated light path system used for fluorescence detection of liquid drops | |
| CN105136665A (en) | Network-based intelligent imaging analyzer for living cell culture in box | |
| CN107037579A (en) | The optical tweezers system of feedback control is combined in a kind of power load and displacement | |
| CN104181089A (en) | Equipment for scanning facial porosity of rock and method thereof | |
| CN108535169A (en) | A kind of internal pH value detection method | |
| JPS61159135A (en) | Particle analyzing device | |
| CN102353818A (en) | Device and method for evaluating neuron-like differentiation degree of PC12 cell | |
| CN217429994U (en) | Nicotinic acid skin reaction image processing system | |
| CN105738254A (en) | Mechanobiological coupling testing system and method | |
| CN104677503A (en) | Method for monitoring laser melting pool in real time | |
| CN106996926A (en) | Tumour cell mitosis process biomechanical characterization monitoring device and monitoring method | |
| CN112393744B (en) | MEMS gyroscope online laser trimming system with frequency measurement function and method | |
| CN111044429B (en) | Vehicle-mounted soil texture information real-time acquisition system | |
| CN110438002A (en) | The wireless multi-channel system of temperature monitoring during a kind of adherent growth for cell | |
| CN103513046B (en) | Micro biological sample measurement system | |
| CN108732103A (en) | A kind of cell detection and sorter based on optofluidic imaging spectral | |
| CN102138783B (en) | Signal acquisition positioning system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170801 |
|
| RJ01 | Rejection of invention patent application after publication |