CN106974947A - A kind of sweet tea extract and its preparation method and application - Google Patents
A kind of sweet tea extract and its preparation method and application Download PDFInfo
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- CN106974947A CN106974947A CN201710237576.3A CN201710237576A CN106974947A CN 106974947 A CN106974947 A CN 106974947A CN 201710237576 A CN201710237576 A CN 201710237576A CN 106974947 A CN106974947 A CN 106974947A
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- sweet tea
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Abstract
The invention discloses a kind of preparation method of sweet tea extract, comprise the following steps：Dry Sweet tea blade is crushed to 25 35 mesh, Sweet tea blade dry powder is obtained；Dry powder is taken, is put in apparatus,Soxhlet's, 3 5h are heated to reflux using n-hexane, degreasing is carried out, after the completion of degreasing, using 85 94 DEG C of 95 115min of backflow of acetone, acetone extract is obtained, is filtered, filtrate heating is concentrated into constant weight, obtains sweet tea extract crude product, wherein, the solid-liquid ratio of dry powder and acetone is 1:21‑1:24；Water is added in sweet tea extract crude product, solid-liquid ratio is 1:21‑1:24, heat so that sweet tea extract crude product fully dissolves, filter while hot, filtrate is placed in 4 DEG C of 25 28h of standing, filtering, dry filter residue, obtain sweet tea extract.The application of the sweet tea extract and the extract that are prepared invention additionally discloses a kind of above method.Sweet tea extract prepared by the present invention can be used for treatment hyperglycaemia.
Description
Technical field
The present invention relates to biomedicine technical field, more particularly to a kind of sweet tea extract and preparation method thereof and should
With.
Background technology
Sweet tea also known as primary calophyllum inophyllum, are difficult plantation, based on wild growth naturally, are grown in high mountain cliff miscellaneous tree clump more
In, generally 1-2 meters of height.Sweet tea nutritive value enriches, with obvious hypoglycemic effect.
Glucose in blood is referred to as blood glucose, and blood glucose must keep certain level to maintain internal each organ and tissue
The need for, when blood sugar concentration exceedes normal value, referred to as hyperglycaemia.Long-term hyperglycaemia can make whole body each histoorgan generation
Lesion, causes the generation of acute and chronic complication.The treatment of current hyperglycaemia, usually drug therapy, but drug therapy can be present
The situation of pharmacological dependence.
How to provide it is a kind of can fundamentally reduce the pure natural substance of blood glucose, be that hypoglycemic therapy field is urgently to be resolved hurrily
One of problem.
The content of the invention
It is an object of the invention to solve at least one above-mentioned problem there is provided a kind of sweet tea extract and preparation method thereof and
Using.
In order to solve the above technical problems, the present invention provides a kind of preparation method of sweet tea extract, comprise the following steps：Will
Dry Sweet tea blade is crushed to 25-35 mesh, obtains Sweet tea blade dry powder；Dry powder is taken, is put in apparatus,Soxhlet's, n-hexane is utilized
3-5h is heated to reflux, degreasing is carried out, after the completion of degreasing, using 85-94 DEG C of backflow 95-115min of acetone, acetone extract, mistake is obtained
Filter, filtrate heating is concentrated into constant weight, obtains sweet tea extract crude product, wherein, the solid-liquid ratio of dry powder and acetone is 1:21-1:24；
Water is added in sweet tea extract crude product, solid-liquid ratio is 1:21-1:24, heat so that sweet tea extract crude product fully dissolves, take advantage of
Heat filtering, filtrate is placed in 4 DEG C of standing 25-28h, and filtering, dry filter residue obtain sweet tea extract.
Wherein, Sweet tea blade is crushed to 30 mesh.
Wherein, n-hexane is heated to reflux 4h, 90 DEG C of backflow 100min of acetone, and the solid-liquid ratio of dry powder and acetone is 1:23.
Wherein, the solid-liquid ratio of sweet tea extract crude product and water is 1:23.
Wherein, water is distilled water.
Wherein, the sweet tea extract of acquisition is subjected to silica gel column chromatography, is eluted, collected using the mixed liquor of chloroform and methanol
Sweet tea extract eluent, is concentrated in vacuo at 35-40 DEG C, sweet tea extract is obtained again, wherein, in mixed liquor, chloroform and first
The volume ratio of alcohol is 4:6.
In order to solve the above technical problems, the present invention provides the sweet tea extract that a kind of above-mentioned preparation method is prepared.
In order to solve the above technical problems, the present invention provides a kind of sweet tea extract as the application of hypoglycemic drug.Specifically
Ground, sweet tea extract is tablet, capsule, granule or powder as the formulation of hypoglycemic drug or health products.
The beneficial effects of the invention are as follows：It is different from the situation of prior art, the preparation method of sweet tea extract of the invention
Comprise the following steps：Dry Sweet tea blade is crushed to 25-35 mesh, Sweet tea blade dry powder is obtained；Dry powder is taken, surname extraction is put
In device, 3-5h is heated to reflux using n-hexane, degreasing is carried out, after the completion of degreasing, using 85-94 DEG C of backflow 95-115min of acetone,
Acetone extract is obtained, filtering, filtrate heating is concentrated into constant weight, obtains sweet tea extract crude product, wherein, the feed liquid of dry powder and acetone
Than for 1:21-1:24；Water is added in sweet tea extract crude product, solid-liquid ratio is 1:21-1:24, heat so that sweet tea extract is thick
Product fully dissolve, and filter while hot, and filtrate is placed in 4 DEG C of standing 25-28h, and filtering, dry filter residue obtain sweet tea extract.Above-mentioned side
Sweet tea extract prepared by method can be used for treatment hyperglycaemia, and sweet tea extract is pure natural substance, over the course for the treatment of, from root
Blood glucose, the side effect of no pharmacological dependence are reduced on this.
Embodiment
Embodiment 1
The preparation method of sweet tea extract comprises the following steps：Dry Sweet tea blade is crushed to 30 mesh, Sweet tea blade is obtained
Dry powder；Dry powder is taken, is put in apparatus,Soxhlet's, 4h is heated to reflux using n-hexane, degreasing is carried out, after the completion of degreasing, utilizes acetone
90 DEG C of backflow 100min, obtain acetone extract, filter, and filtrate heating is concentrated into constant weight, obtains sweet tea extract crude product, wherein,
The solid-liquid ratio of dry powder and acetone is 1:23；Water is added in sweet tea extract crude product, solid-liquid ratio is 1:23, heat so that Sweet tea is carried
Take thing crude product fully to dissolve, filter while hot, filtrate is placed in 4 DEG C of standing 26h, and filtering, dry filter residue obtain sweet tea extract.
In the present embodiment, water is distilled water.The sweet tea extract of the present embodiment can be used for treatment hyperglycaemia, and without medicine
Rely on side effect.
In the present embodiment, the solid-liquid ratio of dry powder and acetone is g/ml, and the solid-liquid ratio of sweet tea extract crude product and water is g/
ml。
Embodiment 2
The preparation method of sweet tea extract comprises the following steps：Dry Sweet tea blade is crushed to 28 mesh, Sweet tea blade is obtained
Dry powder；Dry powder is taken, is put in apparatus,Soxhlet's, 4h is heated to reflux using n-hexane, degreasing is carried out, after the completion of degreasing, utilizes acetone
87 DEG C of backflow 110min, obtain acetone extract, filter, and filtrate heating is concentrated into constant weight, obtains sweet tea extract crude product, wherein,
The solid-liquid ratio of dry powder and acetone is 1:22；Water is added in sweet tea extract crude product, solid-liquid ratio is 1:22, heat so that Sweet tea is carried
Take thing crude product fully to dissolve, filter while hot, filtrate is placed in 4 DEG C of standing 27h, and filtering, dry filter residue obtain sweet tea extract.
In the present embodiment, water is distilled water.The sweet tea extract of the present embodiment can be used for treatment hyperglycaemia, and without medicine
Rely on side effect.
In the present embodiment, the solid-liquid ratio of dry powder and acetone is g/ml, and the solid-liquid ratio of sweet tea extract crude product and water is g/
ml。
Embodiment 3
On the basis of embodiment 1, the sweet tea extract of acquisition is subjected to silica gel column chromatography, chloroform and the mixed liquor of methanol is utilized
Elution, collects and is concentrated in vacuo at sweet tea extract eluent, 37 DEG C, sweet tea extract is obtained again.Wherein, in mixed liquor, chlorine
The imitative volume ratio with methanol is 4:6.
Embodiment 4
On the basis of embodiment 2, the sweet tea extract of acquisition is subjected to silica gel column chromatography, chloroform and the mixed liquor of methanol is utilized
Elution, collects and is concentrated in vacuo at sweet tea extract eluent, 37 DEG C, sweet tea extract is obtained again.Wherein, in mixed liquor, chlorine
The imitative volume ratio with methanol is 4:6.
Embodiment 5
The preparation of sweet tea extract tablet：Sweet tea extract prepared by embodiment 3 or embodiment 4 is broken into directly with single-punch tablet press
Footpath is 7mm, and weight is 350mg piece, produces sweet tea extract tablet.
Embodiment 6
The preparation of sweet tea extract capsule：Sweet tea extract prepared by embodiment 3 or embodiment 4 is packed into No. 1 with 350mg
Capsulae enterosolubilis, produces sweet tea extract capsule.
Embodiment 7
The preparation of sweet tea extract granule：Sweet tea extract prepared by embodiment 3 or embodiment 4, which adds water, is made softwood, mistake
15 mesh sieves are granulated, and sweet tea extract granule is produced after drying.
Embodiment 8
The preparation of sweet tea extract powder：Sweet tea extract prepared by embodiment 3 or embodiment 4 crosses 250 mesh sieves, produces Sweet tea
Extract powder.
Clinical data one：Hyperglycemic patients 200 are chosen, Sweet tea prepared by embodiment 1 or embodiment 2 is taken orally 3 times a day
Extract, continuously takes 6 months.After treatment end, glucose, the level of fructosamine in detection blood samples of patients.Disable Sweet tea
After extract 1 month, the blood glucose value of patient is detected.
Testing result：After treatment end, 200 patient glucoses averagely decline 14.8%, and fructosamine averagely declines 28.1%.
Sweet tea extract is disabled after 1 month, each patient is in the case of healthy diet, and blood glucose value is without big change.
According to testing result, illustrate that sweet tea extract prepared by embodiment 1 and embodiment 2 has the effect for treating hyperglycaemia
With, and over the course for the treatment of, no drugs dependence phenomenon.
Clinical data two：Hyperglycemic patients 200 are chosen, are divided into two groups, each 100 people, first group of implementation for oral administration 3 times a day
Sweet tea extract prepared by example 1 or embodiment 2, second group takes the dihydromyricetin purified orally 3 times a day, and each group continuously takes 6
Individual month.After treatment end, glucose, the level of fructosamine in detection blood samples of patients.
Testing result：After treatment end, first group of patient glucose averagely declines 14.1%, and fructosamine averagely declines
27.8%；After treatment end, second group of patient glucose averagely declines 8.3%, and fructosamine averagely declines 15.5%.
According to testing result, illustrate that sweet tea extract prepared by embodiment 1 or embodiment 2 has more preferably compared with dihydromyricetin
Blood sugar decreasing effect.
Clinical data three：Hyperglycemic patients 200 are chosen, four groups are randomly divided into, each 50 people, first group takes orally 3 times a day
Sweet tea extract prepared by embodiment 1, second group takes sweet tea extract prepared by embodiment 2, the 3rd group daily 3 orally 3 times a day
Sweet tea extract prepared by secondary embodiment for oral administration 3, the 4th group takes sweet tea extract prepared by embodiment 4 orally 3 times a day, and each group connects
It is continuous to take 6 months.After treatment end, glucose, the level of fructosamine in detection blood samples of patients.
Testing result：After treatment end, first group of patient glucose averagely declines 14.6%, and fructosamine averagely declines
27.9%；After treatment end, second group of patient glucose averagely declines 14.5%, and fructosamine averagely declines 27.7%；Treatment end
Afterwards, the 3rd group of patient glucose averagely declines 15.1%, and fructosamine averagely declines 28.5%；After treatment end, the 4th group of patient Portugal
Grape sugar averagely declines 15.0%, and fructosamine averagely declines 28.4%.
According to testing result, illustrate that the refined sweet tea extract of embodiment 3, embodiment 4 has more preferable blood sugar decreasing effect.
In summary, the sweet tea extract that prepared by the present invention can be used for treatment hyperglycaemia, and sweet tea extract is pure natural
Material, over the course for the treatment of, fundamentally reduces blood glucose, the side effect of no pharmacological dependence.
Embodiments of the invention are the foregoing is only, are not intended to limit the scope of the invention, it is every to utilize this hair
The equivalent transformation that bright description is made, or other related technical fields are directly or indirectly used in, similarly it is included in
In the scope of patent protection of the present invention.
Claims (9)
1. a kind of preparation method of sweet tea extract, it is characterised in that comprise the following steps：
Dry Sweet tea blade is crushed to 25-35 mesh, Sweet tea blade dry powder is obtained；
The dry powder is taken, is put in apparatus,Soxhlet's, 3-5h is heated to reflux using n-hexane, degreasing is carried out, after the completion of degreasing, utilized
85-94 DEG C of backflow 95-115min of acetone, obtains acetone extract, filters, filtrate heating is concentrated into constant weight, obtains sweet tea extract
Crude product, wherein, the solid-liquid ratio of the dry powder and acetone is 1:21-1:24；
Water is added in the sweet tea extract crude product, solid-liquid ratio is 1:21-1:24, heat so that sweet tea extract crude product is abundant
Dissolving, is filtered while hot, and filtrate is placed in 4 DEG C of standing 25-28h, and filtering, dry filter residue obtain sweet tea extract.
2. the preparation method of sweet tea extract according to claim 1, it is characterised in that the Sweet tea blade is crushed to 30
Mesh.
3. the preparation method of sweet tea extract according to claim 2, it is characterised in that the n-hexane is heated to reflux
The solid-liquid ratio of 4h, 90 DEG C of backflow 100min of acetone, dry powder and acetone is 1:23.
4. the preparation method of sweet tea extract according to claim 3, it is characterised in that the sweet tea extract crude product with
The solid-liquid ratio of water is 1:23.
5. the preparation method of sweet tea extract according to claim 4, it is characterised in that the water is distilled water.
6. the preparation method of sweet tea extract according to claim 4, it is characterised in that carry out the sweet tea extract
Silica gel column chromatography, is eluted using the mixed liquor of chloroform and methanol, is collected and is concentrated in vacuo at sweet tea extract eluent, 35-40 DEG C,
Sweet tea extract is obtained, wherein, in mixed liquor, the volume ratio of chloroform and methanol is 4:6.
7. the sweet tea extract that the preparation method described in claim 1-6 any one is prepared.
8. sweet tea extract described in claim 7 is used as the application of hypoglycemic drug or health products.
9. application according to claim 8, it is characterised in that the sweet tea extract is used as hypoglycemic drug or health products
Formulation be tablet, capsule, granule or powder.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101367959A (en) * | 2008-10-09 | 2009-02-18 | 中国科学院广州化学研究所 | Uses of tea polysaccharide extract-dihydromyricetin and its esterified matter as anti-oxidant in superpolymer |
| CN104610394A (en) * | 2015-01-30 | 2015-05-13 | 望宏端 | Method for extracting phlorizin with different purity from twigs and green leaves of Malus hupehensis |
-
2017
- 2017-04-12 CN CN201710237576.3A patent/CN106974947A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101367959A (en) * | 2008-10-09 | 2009-02-18 | 中国科学院广州化学研究所 | Uses of tea polysaccharide extract-dihydromyricetin and its esterified matter as anti-oxidant in superpolymer |
| CN104610394A (en) * | 2015-01-30 | 2015-05-13 | 望宏端 | Method for extracting phlorizin with different purity from twigs and green leaves of Malus hupehensis |
Non-Patent Citations (4)
| Title |
|---|
| 北京化学试剂公司: "《化学试剂·精细化学品产品目录》", 31 May 1999 * |
| 山东省食品药品监督管理局: "《山东省中药材标准》", 31 January 2013 * |
| 张雷红等: "《天然药物化学》", 31 January 2017, 中国医药科技出版社 * |
| 汪鋆植等: "湖北海棠抗大蒜病毒活性研究", 《安徽农业科学》 * |
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Application publication date: 20170725 |